LOCUS       NZ_CP104864            11311 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5887597
            Orig. end                         :: 5898908
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..11311
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11311
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11311
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6311](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6311)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(619..633)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     misc_feature    868..877
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            1165..1896
                     /gene="hemQ"
                     /locus_tag="N7E53_RS26605"
     CDS             1165..1896
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="N7E53_RS26605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_033274023.1"
                     /transl_table=11
                     /translation="MSDDATTPAPDRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFFTGRRKDVADLVAGLA"
     gene            complement(1901..3457)
                     /locus_tag="N7E53_RS26610"
     CDS             complement(1901..3457)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (103-476). E-value:
                     1.3e-19. Score: 62.8. Matches aSDomain:
                     nrpspksdomains_N7E53_RS26610_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523451.1"
                     /locus_tag="N7E53_RS26610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_261675800.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-23, bitscore: 81.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALVAAGSLLLTTPAPASPSTSASEARGTAVAAARAASEGID
                     FGPCTEEQELPPTVECGTLSVPLDYARPDGERIRLTVSRSRATGKDPATGRAVPRQGSL
                     VNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSAPLSCTDPATFHQGPA
                     PAPTHPTAAVKRERAARAAAYARGCAERAGDALQHYTSPNNARDLDVLRAALGETRLTF
                     LGSSYGTYVGALYATLFPSHVRRMVFDSAVNPAPERIWYRNGLARSAAFEDRWADFRAW
                     AARHHAVYGLGDTARKVGDSYDVVRERLAREPAGGKVGPAQLHASMLKAAYYDDHWPQR
                     AHALSAYLKGDPAPLVEQAEEQPETAAEAENTRAVYLAVQCNDAPWPTDFAVWDRDNTR
                     LARRAPFGTWDNVWTNLPCASWPAPRQRPLDVRAGAGELPPTLILAAERDAATPYDGAL
                     ELHRRLAGAALVTERDAGSHGLAGGANACVNAHLTAYLLEGRVPGRRASCAPHPAPALR
                     A"
     aSDomain        complement(2030..3148)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS26610_Abhydrolase_1.1"
                     /evalue="1.30E-19"
                     /label="N7E53_RS26610_Abhydrolase_1.1"
                     /locus_tag="N7E53_RS26610"
                     /protein_end="476"
                     /protein_start="103"
                     /score="62.8"
                     /tool="antismash"
                     /translation="LVNNPGGPGVSGMYFPMLGVVPGWKRLAAAYDLVGYAPRGVGRSA
                     PLSCTDPATFHQGPAPAPTHPTAAVKRERAARAAAYARGCAERAGDALQHYTSPNNARD
                     LDVLRAALGETRLTFLGSSYGTYVGALYATLFPSHVRRMVFDSAVNPAPERIWYRNGLA
                     RSAAFEDRWADFRAWAARHHAVYGLGDTARKVGDSYDVVRERLAREPAGGKVGPAQLHA
                     SMLKAAYYDDHWPQRAHALSAYLKGDPAPLVEQAEEQPETAAEAENTRAVYLAVQCNDA
                     PWPTDFAVWDRDNTRLARRAPFGTWDNVWTNLPCASWPAPRQRPLDVRAGAGELPPTLI
                     LAAERDAATPYDGALELHRRLAGAALVTERDAG"
     gene            complement(3557..4339)
                     /locus_tag="N7E53_RS26615"
     CDS             complement(3557..4339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523450.1"
                     /locus_tag="N7E53_RS26615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_193467747.1"
                     /transl_table=11
                     /translation="MFWVPLLLLAWAIAGVVGVRLCRAAVREAAAAGDGAGERRGLTLY
                     EAAFLSGGPARVADLTLVAMARQRRLLLAHTGWATVVDPRGRDDMERTVIGAIGPEGQS
                     RIAPVRKAAATAEAVRTLAERLVRSGLAVPEGDGRTVARALRRTRLAVPAITGLAAVAL
                     LMPVPSQMPRLLVALWFALPLAFTLGCLAIARFELSPASPWASPAGRRLLASARRAAAG
                     DTDDDRAYLTSVAVSGVHAVGEPQVRAAFARREPRVEW"
     misc_feature    4333..4346
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(4446..4461)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.49"
                     /tool="antismash"
     gene            4720..4950
                     /locus_tag="N7E53_RS26620"
     CDS             4720..4950
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N7E53_RS26620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_203549914.1"
                     /transl_table=11
                     /translation="MEDPYATVEIRNPNAHEAVLEVKVHFVDAYGSPLLDTSNQVVVAA
                     KDELAYRVPAKFQYTDRIAHCEVEPVVRAVR"
     gene            complement(5001..6311)
                     /locus_tag="N7E53_RS26625"
     CDS             complement(5001..6311)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130505.1"
                     /locus_tag="N7E53_RS26625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_261675801.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-97, bitscore: 324.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVRLGTGIGWRPEIADAVEAMPGIDWVEVVAENVCPGHLPASL
                     LRLRERGTTVVPHGVSLGLGGAERPDEGRLTALAERASALGSPLVTEHIAFVRAGGPLT
                     ASPGIEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQF
                     LYELADRTGVQLLIDVANLHTNHVNRGEDPAKALAELPLEAIAYVHVAGGFERDGVWHD
                     SHAHPVPRPVLDILTDLASRVSPPGVLLERDENFPPPGELERELDAIRGAVEAGDRARV
                     PSAPEPVREPAAAEPVAGATRERLALAQTAVLSSLVAGTPVPEGFDRTRMRVQARALAA
                     KRADVVAKVAPELPEILGDGYRPAFLAYAQTRPMTGGYRHDAMAFVAHLLHPGRLDDPR
                     TRRALREWHRDRSGPHPRSHRPTTRLARATRRALLRR"
     gene            complement(6555..8618)
                     /locus_tag="N7E53_RS26630"
     CDS             complement(6555..8618)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523447.1"
                     /locus_tag="N7E53_RS26630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="neutral/alkaline ceramidase"
                     /protein_id="WP_261675802.1"
                     /transl_table=11
                     /translation="MTAERPPLRSRRTVLRAGAVTGLGLAGAHLVSPGTAAAATGSGGG
                     YLVGRGISDATGEVAEVGMMGYGRFDQQAAGLHTRLRARAFVVAEQASGRRVLLIVADS
                     PMIFESVHRAVLRRLKQAYGDLYTARNVLITATHTHAGPGGYSHHLLYNTTTFGFHKAT
                     FEAVADGLFEAARNAHEDLAPSELLLSHGTLTGASVNRSKTAFDRNPTADRDHFPDGVD
                     TLTTLLRVEREGRTVGAVNWFPVHGTSMSGDNRLISADNKGYAAYHWEREVHGVDYLAD
                     GTPAFVSAFAQTNSGDMSPNLDLVPPTTPADFEATRTSGLRQYEAAAAQAEAAGTRLSG
                     PVDSRLVYVDLSDVTVRPEFTGDGKTHRTSKPCVGAAMAAGSLEDGPAFPGFDEGENPF
                     WDAISNSIVYTVSPELKQAQAPKDVFVPIGEMNRIYPWVQERVPVMLVRIGGLYLIGIP
                     GEVTVCAGLRLRRTVAGIVGADLDDVLVAGYSNSYFHYVTTPEEYDAQQYEGGSTLFGR
                     WQLPALQQTAADLAVSLRDGTDLPLGPEPPDLTGKALSLQPGVVLDAPPLFRKFGDVLV
                     PPRENYRTGERVEVVFAGAHPSNDLHRGDTYLRIERRDSSGAWRTVADDGDWATRFHWA
                     RDGIAASKVTITWDIPQGTPSGTYRVVYRGDAKSLGGKISSIEGVSTAFQVR"
     misc_feature    complement(8629..8648)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.35"
                     /tool="antismash"
     misc_feature    complement(8631..8645)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.9"
                     /tool="antismash"
     gene            complement(8804..10399)
                     /locus_tag="N7E53_RS26635"
     CDS             complement(8804..10399)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (116-294). E-value:
                     2.8e-15. Score: 48.5. Matches aSDomain:
                     nrpspksdomains_N7E53_RS26635_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130508.1"
                     /locus_tag="N7E53_RS26635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_261675803.1"
                     /sec_met_domain="PF00561 (E-value: 4.8e-25, bitscore: 87.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRRTTAAALLSGAVLLSASLAACGPAGEGGAGGGGGEEDDRGR
                     IWAQSLNWKDCPAPSPLQGGGEASGPLPDGTRWQCATMRAPLDWDRPGGETVDVALIRA
                     ASRADAADRIGSLLFNFGGPGESGVATLPALAPDYEQLRSRYDLVSFDPRGVGASAGVT
                     CLDAEAAQAMAQQDDTPDDGDDEVTELLQARQRYADACERESGRVLPHVGTEDAARDMD
                     LLRHLLGDDELHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQAGG
                     FQLAFDHFAQWCVDTGCALGDTPEEITRLVMELEETLDDEPLSVGGDRVFTGSQLIDAV
                     VQALYLETAWPALEVGLQLLAADEGETLLGLADALSGRHAGGWSDQQDAQTAITCADRS
                     DRYTVEDVLEQAAAFEEASPLFGDTMVWGTLECTDWPVRGKAPHPDVSAPGAPPILLVG
                     NTGDPATPYEGAARMAERLGKGVGVELTYEGEGHGAYDSGNACVRKAVNAYLLDGTVPA
                     AGTVCEREPLQGKE"
     aSDomain        complement(9518..10051)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS26635_Abhydrolase_1.1"
                     /evalue="2.80E-15"
                     /label="N7E53_RS26635_Abhydrolase_1.1"
                     /locus_tag="N7E53_RS26635"
                     /protein_end="294"
                     /protein_start="116"
                     /score="48.5"
                     /tool="antismash"
                     /translation="LLFNFGGPGESGVATLPALAPDYEQLRSRYDLVSFDPRGVGASAG
                     VTCLDAEAAQAMAQQDDTPDDGDDEVTELLQARQRYADACERESGRVLPHVGTEDAARD
                     MDLLRHLLGDDELHYFGISYGTELGGVYAHLFPRHVGRAVLDGVVDPTLDQMEGALAQA
                     GGFQLAFDHFAQWCV"
     gene            complement(10467..11186)
                     /locus_tag="N7E53_RS26640"
     CDS             complement(10467..11186)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130509.1"
                     /locus_tag="N7E53_RS26640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_033274017.1"
                     /transl_table=11
                     /translation="MNDDRTTPAPDSPFRPDPGDRDAAPQFVLPLVVRLERGSPPARTD
                     ALETAARAVLVMLTDERSVGDGEWARAIRDWQDARIRKVVRRARGAEWRRAEALPGITV
                     TGKSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPDPVPPADPGVPTLWMNPELEMSAG
                     KAMAQAGHGAQLAWWELPHAARAAWHAAGFPLAVRTADPADWPALTGAGLPTVRDAGFT
                     EIAPGSCTVVADHPALR"
ORIGIN
        1 cctgcgcccc atgccgaagg gacacgtcat gggcgtcccc ggcaccgccg ccgccctcgc
       61 cggcgtgctc tccgacgagg gcctcgcccg gatcgagcgg gacgccgaac tgccgcccac
      121 cgagatcggc gacgacgtcg ccatcggcga gtacgtcgcc gagaggttcg gccgtgaggt
      181 cgtcgaccgg ctcgtggagc ccctgctcgg cggggtgtac gcgggggacg cgtaccgcat
      241 ctcgatgcgc tcggccgtcc cgggcctctt cgacgccgcc cgcgcgcatg ccaccctgac
      301 cgaggcggtc cgcgccgtgc aggcgaagac cgccgccgcg cagcggacag gacccgtctt
      361 cacgggcgtc gcgggcggtg tgggcaccct gccgctcgcg gtcgccgact tcctgcgcgc
      421 ccggggcgtc cgcatcctca ccggcacccc ggtcaccgag ctgcgccgcg aacccgacga
      481 cggctggcgg ctcgtcgccg gcggcgaggt gctgcacgcc gacgcggtcg tcgtcgccgc
      541 gcccgccccg gccgccgcgg acctgctgcg cgccgaggcg ccgggcgccg ccgccgagct
      601 gtccgccgtc gagtacgcct cgatggcgct gatcaccctc gcctaccggc gcgacgaggc
      661 acacctgccc gagggcagcg ggttcctggt gccgcccgtg gagggccgca gcatcaaggc
      721 gtccacgttc gcctcccgca aatggggctg gatcgccgag gagaacccgg acctggccgt
      781 cgtccgcacc tccgtgggcc gccacggcga gacggagctc ctgcaccgcg acgacgacga
      841 gctggtggag ctgtcccggc acgacctgaa ggcggccacc ggcctggacg cctccccggt
      901 cgccgcccgc gtcacccgct gggacgacgg cctgccgcag tacccggtcg gccaccacgc
      961 gcgcgtcgcc cgcgtccgcg cccacctcgc cggcctgtcc gggctggccg tctgcggcgc
     1021 cgcgtacgac ggcgtcggcg tccccgcgtg catcgcgagc gcctacgccg ccgccgacca
     1081 gatccagggt gacctgcgcg ctgtgcagca gctcaccgcg cacccggtgc aaagccttca
     1141 cggaggagca ggagaataag ggacatgagt gacgacgcca ccacccccgc gcccgaccgg
     1201 atccccaaca agggcaagct ggccaaggac ctcaacgagg tcatccgcta caccctgtgg
     1261 tccgtgttca agctgaagga cgtcctgccg gaggaccgcg cgggctacgc cgacgaggtc
     1321 caggagctgt tcgaccagct cgccgccaag gacgtcacca tccgcggcac ctacgacgtg
     1381 tcgggcctgc gcgcggacgc cgacctcatg atctggtggc acgcggagac cgccgaccag
     1441 ctccaggagg cctacaacct cttccgccgc accaggctgg gccgcgcgct ggagccggtc
     1501 tggtcgaaca tggcgctgca ccgccctgcc gagttcaacc gctcgcacat cccggcgttc
     1561 ctcgccgacg agacgccccg cgactacgtg agcgtctacc cgttcgtgcg ctcctacgac
     1621 tggtacctgc tgccggacga ggaccgccgc cgcatgctcg cggaccacgg caagatggcc
     1681 cgcggctacc cggacgtgcg cgccaacacg gtcgcgtcgt tctccctcgg cgactacgag
     1741 tggatcctcg ccttcgaggc cgacgagctg taccgcatcg tcgacctgat gcgtcacctg
     1801 cgcgcctccg aggcccgtat gcacgtccgt gaggaggtcc cgttcttcac gggccgccgc
     1861 aaggacgtcg cggacctggt cgccggtctg gcctgacctc tcaggcccgg agagcgggcg
     1921 ccgggtgcgg tgcgcaggac gcgcgccggc ccggcacccg cccctccagc agatacgccg
     1981 tcaggtgcgc gttcacgcac gcgttggcgc cgcccgcgag gccgtggctg ccggcgtccc
     2041 gctccgtcac cagcgccgcc ccggcgagcc gccggtgcag ctccagcgcc ccgtcgtagg
     2101 gggtcgccgc gtcccgttcg gccgccagga tcagggtcgg cggcagctcg cccgcaccgg
     2161 cgcgcacgtc gagcggccgc tgccggggcg ccggccagga ggcgcagggc aggttcgtcc
     2221 acacgttgtc ccacgtcccg aacggcgccc ggcgcgccag ccgcgtgttg tcccggtccc
     2281 acaccgcgaa gtccgtcggc cagggggcgt cgttgcactg aacggccagg tacaccgcac
     2341 gcgtgttctc cgcctcggcc gccgtctcgg gctgctcctc cgcctgctcc accagcggag
     2401 cggggtcgcc cttcagatac gccgacaggg cgtgcgcgcg ctgcggccag tggtcgtcgt
     2461 agtacgccgc cttcagcatc gacgcgtgca gctgcgccgg accgaccttc ccgcccgccg
     2521 gctcccgcgc cagccgctcc cgcacgacgt cgtagctgtc gcccaccttc cgcgcggtgt
     2581 cgcccagccc gtacacggcg tggtggcggg cggcccacgc ccggaagtcc gcccagcggt
     2641 cctcgaacgc ggccgaccgg gcgaggccgt tgcggtacca gatccgctcc ggcgccgggt
     2701 tcaccgccga gtcgaacacc atccgccgca cgtgcgaggg gaacagcgtc gcgtacagcg
     2761 ccccgacgta cgtgccgtac gacgagccga ggaacgtcag ccgggtctcg cccagcgcgg
     2821 cgcgcagcac gtccaggtcg cgtgcgttgt tcggcgaggt gtagtgctgg agggcgtcgc
     2881 cggcccgctc ggcgcacccc cgcgcgtacg ccgccgcgcg ggccgcgcgc tcccgcttca
     2941 ccgctgccgt cgggtgcgtg ggcgcgggcg cggggccctg gtggaaggtg gccgggtcgg
     3001 tgcacgacag cggggccgaa cggcccaccc cacgcggcgc gtagccgacc aggtcgtacg
     3061 ccgcggccag ccgcttccag cccgggacca cgcccagcat cgggaagtac atgccgctga
     3121 cgcccggccc gcccgggttg ttcaccaggg agccctggcg gggcaccgcg cgcccggtcg
     3181 cggggtcctt cccggtggcc cgtgagcggc tcacggtgag ccggatgcgc tcgccgtccg
     3241 ggcgggcgta gtcgagcggg acgctcagcg tgccgcactc caccgtgggc ggcagctcct
     3301 gctcctcggt gcacgggccg aagtcgatcc cctccgaggc cgcccgcgcg gcggcgaccg
     3361 cggtgccgcg cgcctcggac gcggacgtgg agggggacgc gggtgcgggg gtggtgagga
     3421 gcaaggaccc ggcggccacg agggcggcgg ttctcatcgg tgaggtgatc ccttcggtgc
     3481 gctgtggcga caaaagggat gttttcggtg tgcgtggcgc cgggcaagga ccgcccgccg
     3541 ggtgtcggcg acgggttcac cactccacgc gcggctcccg gcgcgcgaag gcggcgcgta
     3601 cctgcggctc gcccaccgcg tgcaccccgc tcaccgcgac ggacgtgagg tacgcgcggt
     3661 cgtcgtcggt gtcgccggcc gccgcccgtc gcgcggaggc gagcagacgc cggcccgccg
     3721 gggacgccca gggtgaggcg gggctcagct cgaaacgggc gatggcgaga cagcccagcg
     3781 tgaacgccag cggcagcgcg aaccacaggg cgaccagcag acgcggcatc tgcgacggca
     3841 ccggcatgag cagcgccacg gcggcgagac cggtgatcgc cggcaccgcg agacgcgtcc
     3901 ggcgcaacgc ccgcgccacg gtccgcccgt cgccctccgg cacggcgagc ccggaccgca
     3961 ccagccgttc cgccagcgtc cgcaccgcct ccgcggtcgc cgcggccttc cgcaccggcg
     4021 ctatgcgcga ctggccctcc ggcccgatcg cccctatcac cgtgcgctcc atgtcgtccc
     4081 gcccgcgcgg gtcgacgacg gtggcccagc cggtgtgcgc gagcagcagc cgccgctgac
     4141 gggccatcgc gacgagcgtg aggtcggcga cccgcgcggg cccgccggac aggaacgccg
     4201 cctcgtacag cgtgagcccc cgccgctctc ccgcaccgtc accggcagcc gccgcctcac
     4261 gcaccgcggc ccggcacagc cgcaccccga cgaccccggc gatcgcccag gcaagcagca
     4321 ggagaggtac ccagaacatg agttgtttgt aagggacatg gtcgcgaaaa caccatgcct
     4381 tgttcacgat ccggacacgg cgttgcggat atgtgacgtt ccgcggaacc gccctgtgcc
     4441 attctgctga cgatcatcag tgggaggggc ggcacgatgc gggtcaaagg aagtctgcgc
     4501 cgatggagct ggggggtgac ggtgacagcc gtggtgctgg cggccggttg ctcgcccggc
     4561 agtgactccg gtgcccccgt gaccgtcgcg ggtgggaacg gcgtcagtgc acagcccacg
     4621 cccacagcca cagccacagc cacagccacg gtgtccgccg ccacaagccc agccccgccc
     4681 ctgaaggacg gaaccgccgt actcgtccgc tgcgcggaca tggaggaccc gtacgcgacc
     4741 gtggagatca ggaatcccaa cgcacatgag gccgtgctgg aggtgaaggt ccacttcgtc
     4801 gacgcgtacg gctcaccgct gctggacacc agcaatcagg tggtcgtcgc cgcgaaggac
     4861 gagcttgcgt accgggtgcc cgccaagttc cagtacacgg acaggatcgc gcactgcgag
     4921 gtggaacccg tggtacgggc cgtccgttga agtgcggtgt gtccgagggc tgacggacga
     4981 acacccggtg cgggccggtg tcaacggcgc agcagggccc ggcgggtcgc tcgggccagg
     5041 cgggtcgtcg ggcggtggga gcgggggtgg gggcccgagc ggtcgcggtg ccactcgcgc
     5101 agggcgcggc gcgtgcgggg gtcgtcgagg cggccggggt gcaggaggtg tgccacgaag
     5161 gccatcgcgt cgtgccggta gccgcccgtc atcgggcggg tctgcgcgta cgcgaggaag
     5221 gcgggccggt agccgtcgcc gaggatctcc ggcagctccg gggccacctt cgcgacgacg
     5281 tccgcccgct tggcggccag cgcccgcgcc tgcacccgca tccgcgtccg gtcgaaccct
     5341 tcgggcaccg gcgtccccgc gaccagcgag gacagcaccg cggtctgggc gagcgccagc
     5401 cgctcccggg tggccccggc aacgggctcg gcggcggccg gctcccgtac gggctccggc
     5461 gccgaaggca cccgggcgcg gtcgcccgcc tccaccgccc cgcggatcgc gtccagttcg
     5521 cgctccagct cccccggcgg cgggaagttc tcgtcccgtt ccagcagcac gcccggcggc
     5581 gagacgcggg aggcgaggtc cgtgaggatg tcgaggaccg gccgcggcac cgggtgcgcg
     5641 tggctgtcgt gccacacgcc gtcccgttcg aacccgcccg cgacatggac gtacgcgatg
     5701 gcctccaggg gcagttcggc gagcgccttg gccgggtcct cgccgcggtt gacgtggttg
     5761 gtgtgcaggt tggcgacgtc gatgaggagt tggacgccgg tgcggtcggc cagctcgtac
     5821 aggaactgtc cctcggtcat ctcctcgccc ggccaggaga acagcgcggc gatgttctcc
     5881 acggcgagcg gcaccgggag ggcgtcctgg gcgatgcgga cgttctcgca cagcacgtcg
     5941 agggcgtcgc gggtgcgcgg caccggcagc aggtgcccgg cctcgatgcc gggggacgcg
     6001 gtcagcggac cgcccgcccg cacgaacgcg atgtgctcgg tgaccagggg cgaccccagc
     6061 gccgatgccc gctcggcgag cgcggtgagc cgcccctcgt cggggcgttc ggcgccgccc
     6121 agccccaggg agaccccgtg cggcacgacg gtcgtgccac gctcgcgcag ccgcagcagc
     6181 gacgcgggca gatggccggg gcagacgttc tccgccacca cctcgaccca gtcgatgccc
     6241 ggcatcgcct ccaccgcgtc cgcgatctcc ggacgccacc cgatcccggt ccccagccgc
     6301 accgtcgtca tccccgtccc ctccttcacc ccggacgttc ccgcccggcc cccgatccgt
     6361 ccgcacgggc gtgccggacg tgccggggat atgacccacg gctcggcgcc cgaaccgtcc
     6421 tgcggccgcg ttcagaggaa catttgaggt tgggggaggg ccgtcggtgg aacgccggcc
     6481 cccgcacgcg tgggtggtgg cgggcggcgg gacgagcgtc gtgagccgtg tgatcatccg
     6541 tcggccggac aggctcaccg cacctggaac gccgtcgaca caccctcgat cgacgagatc
     6601 ttcccgccga ggctcttcgc gtcgccgcgg tagacgacgc ggtaggtgcc cgagggggtg
     6661 ccctggggga tgtcccacgt gatggtcacc ttggacgcgg cgatgccgtc gcgggcccag
     6721 tggaagcggg tcgcccagtc gccgtcgtcg gcgacggtgc gccacgcgcc ggaggagtcc
     6781 cgccgttcga tccgcaggta ggtgtcgccg cggtgcaggt cgttgctcgg atgggcgccg
     6841 gcgaagacga cctcgacgcg ttccccggtc cggtagttct cccggggcgg caccagtacg
     6901 tcgccgaact tccggaagag gggcggcgcg tcgagcacca cgcccggctg gagcgacagc
     6961 gccttgccgg tcaggtccgg cggctccggc ccgagcggca ggtccgtgcc gtcgcgcagg
     7021 gagaccgcga ggtcggccgc ggtctgctgg agcgcgggga gctgccagcg cccgaacagg
     7081 gtgctgccgc cctcgtactg ctgggcgtcg tactcctccg gcgtggtcac gtagtggaag
     7141 taggagttgc tgtacccggc gaccaggacg tcgtcgaggt ccgcgccgac gatcccggcg
     7201 accgtgcggc gcagccgcag gcccgcgcag acggtgacct cgccaggaat gccgatgagg
     7261 tacaggccgc cgatgcggac cagcatcacc ggcacccgct cctgcaccca cgggtagatt
     7321 cggttcatct ccccgatcgg cacgaagacg tccttcggcg cctgcgcctg cttcagctcg
     7381 ggggacaccg tgtagacgat tgagttcgag atcgcgtccc agaaggggtt ctcgccctcg
     7441 tcgaagccgg ggaaggcggg cccgtcctcc aggctgcccg ccgccatcgc cgcgccgacg
     7501 cacggcttgg acgtgcggtg cgtcttcccg tcgccggtga actccgggcg caccgtgacg
     7561 tcggagaggt ccacgtacac cagccgcgag tcgaccggcc cggacagccg cgtgcccgcc
     7621 gcctccgcct gggccgcggc cgcctcgtac tgccgcagtc cgctggtgcg ggtggcctcg
     7681 aagtcggcgg gcgtggtggg cggcaccagg tccaggttgg gcgacatgtc gccgctgttg
     7741 gtctgggcga acgccgacac gaacgccggt gtgccgtcgg cgagatagtc cacgccgtgg
     7801 acctcgcgct cccagtggta ggccgcgtag cccttgttgt ccgcgctgat caggcggttg
     7861 tcgccggaca tgctggtgcc gtgcaccggg aaccagttga ccgccccgac cgtgcggccc
     7921 tcccgctcca cccgcagcag ggtggtcagg gtgtccacgc cgtccgggaa gtggtcgcgg
     7981 tcggccgtcg ggttgcggtc gaaggcggtc ttcgagcggt tgacgctcgc cccggtcaac
     8041 gtgccgtgac tgagcagcag ttcggacggc gcgagatcct cgtgagcgtt gcgcgccgcc
     8101 tcgaacaggc cgtccgccac cgcttcgaag gtcgccttgt ggaagccgaa cgtcgtggtg
     8161 ttgtacagga gatggtgcga gtagccgccg gggcccgcgt gggtgtgggt ggcggtgatg
     8221 agcacgttcc gcgcggtgta caggtcgccg tacgcctgct tcagccggcg cagcaccgcc
     8281 cggtgcacgc tctcgaagat catgggggag tcggcgacga tcagcagcac acggcgcccg
     8341 ctcgcctgct cggccacgac gaacgcccgt gcccgcagcc gggtgtggag tcccgccgcc
     8401 tgctggtcga agcggccgta ccccatcatg ccgacctcgg cgacctcacc cgtcgcgtcg
     8461 gagatgcccc ggccgacgag gtacccgccg ccgcttccgg tggcggcggc agcggtgccc
     8521 ggtgagacca gatgcgcgcc cgcgaggccg agtccggtca cggcgcccgc cctcaggacg
     8581 gtccggcggc tgcgcaaggg aggacgttcc gcggtcatac gctgctcctc agggtcggtc
     8641 ggccccccgg caacgaaaca tgaacaacat tcaagttccg cgcctcattg gtacaccggc
     8701 cgccatggac acggcaagag tcgtgcgcgc atgacgcggt cggcccggtg gatccgtgtc
     8761 tccacgcacc caccgggccg gctcccgcct caggacagcg gcgtcactcc ttgccctgga
     8821 gcggctcccg ctcgcacacg gtccccgccg ccggcaccgt gccgtccagc aggtacgcgt
     8881 tcacggcctt ccgcacacag gcgttgccgg agtcgtacgc gccgtggccc tcaccctcgt
     8941 aggtgagttc caccccgacg cccttgccca gccgctccgc catgcgcgcg gcgccctcgt
     9001 acggcgtcgc ggggtcgccg gtgttgccga cgagcaggat cggcggcgcg cccggcgcgc
     9061 tcacgtccgg gtgcggggcc ttgccccgca ccggccagtc ggtgcactcc agcgtgcccc
     9121 acaccatggt gtcgccgaag agcggggacg cctcctcgaa cgcggcggcc tgttccagca
     9181 cgtcctccac ggtgtagcgg tcgctgcggt cggcgcaggt gatggccgtc tgggcgtcct
     9241 gctggtcgct ccagccgccc gcgtgacggc cgctgagcgc gtccgcgagg ccgagcaggg
     9301 tctcgccctc gtcggcggcg aggagttgca gacccacctc cagcgcgggc caggcggtct
     9361 ccaggtagag cgcctggacg acggcgtcga tcagctggga gccggtgaac accctgtccc
     9421 cgccgacgga gaggggttcg tcgtccaggg tctcctccag ctccatgacg agccgggtga
     9481 tctcctccgg ggtgtcgccg agggcgcagc cggtgtccac gcaccactgc gcgaagtggt
     9541 cgaaggcgag ctggaaaccg cccgcctgcg cgagcgcccc ctccatctgg tccagcgtcg
     9601 ggtcgacgac gccgtccagg acggcgcggc cgacgtgccg ggggaacaag tgggcgtaca
     9661 cgccgccgag ttcggtgccg taggagatcc cgaagtagtg cagctcgtcg tcgccgagca
     9721 gatggcgcag caggtccatg tcgcgggcgg cgtcctcggt gcccacgtgc ggcagcacgc
     9781 ggcccgactc ccgctcgcag gcgtccgcgt accgctgccg cgcctggagc agctcggtca
     9841 cctcgtcgtc gccgtcgtcg ggggtgtcgt cctgctgggc catcgcctgc gccgcctcgg
     9901 cgtccaggca ggtgaccccg gcgctcgccc cgaccccgcg cgggtcgaag ctcaccaggt
     9961 cgtagcggga acggagttgc tcgtagtcgg gggcgagggc gggcagggtg gccacgccgg
    10021 actcgccggg cccgccgaag ttgaacagca gcgagccgat gcggtcggcg gcgtccgcgc
    10081 gggacgccgc ccggatcagg gcgacgtcga cggtctcacc gccgggtctg tcccagtcca
    10141 ggggcgcccg catggtggcg cactgccagc gggtgccgtc cggcaacggt ccggacgcct
    10201 caccgccgcc ttgcaggggg gacggtgcgg ggcagtcctt ccagttcagg gactgcgccc
    10261 atatccgtcc gcggtcgtcc tcctcgccgc ctccgcctcc ggccccgccc tcgccggccg
    10321 gaccgcaggc ggccagggag gcggagagca ggacggcgcc ggacagcagg gcagcggctg
    10381 tcgtacgtcg tgtcggcatg cgcccagcct ccggcgggcc gacggcagcc gcacggccgc
    10441 acaacccatc cgggggacgc cccgggtcag cgcagcgcgg ggtggtccgc gaccacggtg
    10501 cacgagccgg gggcgatctc cgtgaagcca gcgtcgcgca ccgtcggcag gccggcaccg
    10561 gtcagcgcgg gccagtcggc ggggtcggcg gtgcgcacgg cgagcgggaa gcccgccgcg
    10621 tgccaggccg cgcgggccgc gtgcggcagt tcccaccagg cgagttgggc gccgtgcccg
    10681 gcctgggcca tcgccttgcc ggcggacatc tccagctccg ggttcatcca gagcgtgggc
    10741 acgccggggt ccgcgggcgg caccgggtcc gggtcgtcga ggtcggtgcc ggacacctgg
    10801 agccgggcca ggtccttggg ccagccgtcc agcggcaccg gcgggaagac gcgtacctcg
    10861 gccgacttcc cggtcacggt gatccccggg agcgcctccg cgcgccgcca ctccgcgccg
    10921 cgcgcccgcc gcaccacctt gcggatccgg gcgtcctgcc agtcgcggat cgcccgcgcc
    10981 cactcgccgt cgcccaccga ccgctcgtcg gtcagcatca ccagcaccgc gcgggccgcc
    11041 gtctccagcg cgtccgtccg cgcgggcggc gagccgcgct cgagacggac caccagcggc
    11101 agcacgaact gcggcgccgc gtcgcggtca cccgggtcgg gtcggaaggg gctgtcgggg
    11161 gcgggtgtcg tccggtcgtc gttcacccgc ccagtgtgcc aagcgggacg ggcagggcga
    11221 cgggcggcac cggcggacat caaggggcgc ggagggggca tccgggtggg ggagaggatg
    11281 gtgcgcgatg actcaggcgg tgccgcagcg t
//
