LOCUS       NZ_CP104864            22494 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4053911
            Orig. end                         :: 4076405
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..22494
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="10"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22494
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22494
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12494](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12494
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(416..431)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.51"
                     /tool="antismash"
     misc_feature    complement(710..724)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.68"
                     /tool="antismash"
     misc_feature    complement(720..737)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            2437..3642
                     /locus_tag="N7E53_RS18605"
     CDS             2437..3642
                     /EC_number="2.7.7.7"
                     /GO_component="GO:0009360 - DNA polymerase III complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674051.1"
                     /locus_tag="N7E53_RS18605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="WP_033274893.1"
                     /transl_table=11
                     /translation="MTVWDDLVGQERVSEQLAAAARDADAFVTAAASDAPPPEASRMTH
                     AWLFTGPPGAGRNQAARAFAAALQCVSPDRALGGAPGCGFCDGCHTALVGTHADVSTVA
                     AVGAEILVKDMRDTVRKSFTAPANGRWQVILVEDAERLNEKSANAVLKAVEEPAPRTVW
                     LLCAPSVEDVLPTIRSRCRHLVLSTPSVEAVADMLVRREGIEPDVAASVARATQGHVDR
                     ARRLATDPAARARRATVLKLPQRVEDVGGALRAAQELVDAAAEDAKQLAEEMDGKETED
                     LKAALGAAQGGRLPRGTAGVVKDLEDMQKRRRTRTQRDSLDVALADLTSFYRDVLALQL
                     GSRIALANTDAEDALERLARSSTPESTLRRIEAIAACGEALDRNVAPLLAVEAMTMALR
                     AG"
     gene            3767..5356
                     /locus_tag="N7E53_RS18610"
     CDS             3767..5356
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (114-501). E-value:
                     7.7e-24. Score: 76.6. Matches aSDomain:
                     nrpspksdomains_N7E53_RS18610_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975395.1"
                     /locus_tag="N7E53_RS18610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_230228999.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-34, bitscore:
                     117.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTRRTPRRTRTAAALFTAAALLSAGCSSGTSTNTTASSPVAGAA
                     AALAPLPGSTPAALAEYYAQEPAWRDCGVPGFQCATVKAPLDYAEPSAGDVRLAVARKK
                     ATGPGKRLGSLLVNPGGPGGSAVDYLQMYAGIGYPEEVRARYDMVAVDPRGVARSEPVE
                     CLDGRAMDAYTRTDTTPDDEKETETLVSAYRTFAEGCGAKAPKLLRHVSTVEAARDMDV
                     VRAVLGDEKLSYVGASYGTLLGATYAGLFPERTGRLVLDGAMDPSLSARRLNLEQTEGF
                     ATAFTAFAKDCVRRSDCPLGGKGTSPEKVGENLKAFFEKLDARPLPAGDADGRELTESL
                     ATMGVIAAMYDEAAWQRLREALTSAMKENDGAGLLVLSDSYYERDAGGAYSNLMSANAA
                     VNCLDLPPAFETPEQVREALPDFEKASPVFGEGLAWASLNCAYWPVRATGEAQRIEAKG
                     AAPIVVVGTTRDPATPYRWAEGLAGQLSSARLLTYDGDGHTAYGRGSSCVDSAINTYLI
                     EGTPPADGKRCS"
     aSDomain        4109..5269
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS18610_Abhydrolase_1.1"
                     /evalue="7.70E-24"
                     /label="N7E53_RS18610_Abhydrolase_1.1"
                     /locus_tag="N7E53_RS18610"
                     /protein_end="501"
                     /protein_start="114"
                     /score="76.6"
                     /tool="antismash"
                     /translation="LLVNPGGPGGSAVDYLQMYAGIGYPEEVRARYDMVAVDPRGVARS
                     EPVECLDGRAMDAYTRTDTTPDDEKETETLVSAYRTFAEGCGAKAPKLLRHVSTVEAAR
                     DMDVVRAVLGDEKLSYVGASYGTLLGATYAGLFPERTGRLVLDGAMDPSLSARRLNLEQ
                     TEGFATAFTAFAKDCVRRSDCPLGGKGTSPEKVGENLKAFFEKLDARPLPAGDADGREL
                     TESLATMGVIAAMYDEAAWQRLREALTSAMKENDGAGLLVLSDSYYERDAGGAYSNLMS
                     ANAAVNCLDLPPAFETPEQVREALPDFEKASPVFGEGLAWASLNCAYWPVRATGEAQRI
                     EAKGAAPIVVVGTTRDPATPYRWAEGLAGQLSSARLLTYDGDGHTAY"
     tRNA            5476..5549
                     /anticodon=(pos:4059421..4059423,aa:Thr,seq:cgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="N7E53_RS18615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Thr"
     gene            5476..5549
                     /locus_tag="N7E53_RS18615"
     gene            complement(5606..6307)
                     /locus_tag="N7E53_RS18620"
     CDS             complement(5606..6307)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="N7E53_RS18620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_193468054.1"
                     /transl_table=11
                     /translation="MTVNLFVVSDNPVSLAGLEAIVDQDEECALVGRATDDSDDLRCLS
                     DLAGTALHPDVVVLSEPCSSRTVSARVRHIASAFPDEASRPHTIVVAQNDDDQMIVSAL
                     RAGVNGYLSRLGSADELLRSLKVVARGGAAFCPSVAARMSRYFSAMQGLNDSVGFPGLT
                     SRELEILELLAGGMGNRQIARRLFLAEKTVRNYVSRIFTKLEVHDRATAVVMARDAGFG
                     EPAGELVRNSA"
     misc_feature    complement(6696..6710)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.18"
                     /tool="antismash"
     gene            6788..7927
                     /locus_tag="N7E53_RS18625"
     CDS             6788..7927
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958865.1"
                     /locus_tag="N7E53_RS18625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_193468053.1"
                     /transl_table=11
                     /translation="MGEGSRRRAIVLDGAAACAFTLLPQVSLLRPAVDGGTGGGVLWLL
                     SLGAGLPLVVRRLWPVPVFVLVLAAACGALAAGLGPAAFLAAAYAVYTVATTRRRDPGV
                     SAVAVAGLCAAVAALLTLSGGQSHQGGSTAVQAVFGLLVLGATWAAGSAVRERRRSTRR
                     AIEHAAEQAKTEERLRIARDLHDVVTHSVGLIAVKAGIANHVVATRPEEAKEALAVIED
                     VSRRALRDMRATLTVLRGEDRSDAGDLRPARGLADLPALLGTAEAAGVRVELRTRYDQE
                     PPEGVALAAFRIVQEALTNVVKHAAPTGCRVDVTAREGVLTVEVTDDGPAPGRRTTVPG
                     GGMGLVGMKERTAAHGGTLRAGPRPGGGFRVTATLPFQV"
     gene            8046..8948
                     /locus_tag="N7E53_RS18630"
     CDS             8046..8948
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136293.1"
                     /locus_tag="N7E53_RS18630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_198427550.1"
                     /transl_table=11
                     /translation="MLAERLTKRYGPATVVDDLSFTVRPGVVTGFLGPNGAGKSTTMRM
                     MLGLTRPDSGTATVGVRPYRDLTYPLRHVGALLETSAPHRGMTAVGHLSWLARSNRIPR
                     RRVDEVLDAVGLTGAARQRVGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDAEGIR
                     WLRELLRAKAAEGRTVLVSSHLMAEMAQVADELIVISRGRLLAETSVSEFIGRHGRTFV
                     RVRTPEPLRAAQEFQAQGAASVRRAADGGLEVDGLPAGEVNRIAAAAGVPVEELSTHTG
                     SLEETFLKLVDDGGDPTHV"
     gene            8941..9693
                     /locus_tag="N7E53_RS18635"
     CDS             8941..9693
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="N7E53_RS18635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_261675383.1"
                     /transl_table=11
                     /translation="MSETLAAARAEFTKMRAMRATSVALLLFVAVSVFIAALGGWSAKG
                     AIESDNPGLRSDFTPEQAGLDGILYGQLALIVFGVLMMSGEYTSGMMRVSLLAVPRRGR
                     LYLAKTAVTAAAALAVALPVTVVSYLVTQLALGPHGSTLDADGVPRALAGGVVYLTLMS
                     LFAVGVAAAARSAVLPLAVLLPLVLVGSQILSVIGATKEVARWFPDRAGAQMLTVDSGD
                     AVTGLVVLLAWTAAALTAGWVRHRSWDG"
     gene            9810..9938
                     /locus_tag="N7E53_RS18640"
     CDS             9810..9938
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103079659.1"
                     /locus_tag="N7E53_RS18640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aborycin family tricyclic lasso peptide"
                     /protein_id="WP_106963308.1"
                     /transl_table=11
                     /translation="MTAIYEPPALQEIGDFDELTKCLGIGSCNDFAGCGYAVVCFW"
     gene            10001..11812
                     /locus_tag="N7E53_RS18645"
     CDS             10001..11812
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067372303.1"
                     /locus_tag="N7E53_RS18645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_037640855.1"
                     /sec_met_domain="Asn_synthase (E-value: 2.1e-37, bitscore:
                     128.2, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFTVLPDCPAGAALADRVAAPKRIDHASGRPWIVGDWAVGEATV
                     TEAGIRRLAVFGHTRPDDAGTVSALGRMRSLHDVDRVASRLPGVFHLVASLDGAVRLQG
                     SVAGVRQVFTTRVDGVTVAASAVDPLLRLTGAGLDETLLAARLLAPGGAPWPLSLRPVR
                     RGVDALPTGHWLELGADGRARSVRWWELPEATLSLEEGAGAVRSALTDALATRVAPDRT
                     ISADLSGGLDSTTLCFLADAAGADLVTYHVMPLDEANEDTAWARKAAAHLPHARHHMLA
                     ADRAANLFDVGYTADTADAAPEGPSTWASGLAHIRDLAGRATAEGAALHLSGFGGDELF
                     GRMPACAWSLARIRPADGLRLVNRYRLANRWPWRSTLRQLADRSTFAQNLIEVAGRITA
                     PPPPVNEPDFGWVFAPRMPAWATPDAVATVRDLLTTAAERTPEPLDADRARHQALSSVV
                     FEGNTVRQVNTAVAGTGLVWEAPFLDDRVLEAAFATRIDERLAAGRFKPLLTTAVRGLV
                     PDDFLARRDKGEFSAETFRGIERNRDRILDLCEDSLLARLGLVDPVAFRSAVLNPGPMS
                     HHLQPIQTTVACESWLRAHPQDSGENR"
     aSDomain        11809..11979
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_N7E53_RS18650_Lasso_Fused_RRE.1"
                     /evalue="4.50E-09"
                     /identifier="RREFam003.1"
                     /label="N7E53_RS18650"
                     /locus_tag="N7E53_RS18650"
                     /protein_end="57"
                     /protein_start="0"
                     /score="27.3"
                     /tool="antismash"
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDG
                     DTPAAAAASLSE"
     gene            11809..12072
                     /locus_tag="N7E53_RS18650"
     CDS             11809..12072
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033530.1"
                     /locus_tag="N7E53_RS18650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_037640857.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.1e-06,
                     bitscore: 27.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.9e-22,
                     bitscore: 76.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 6.5e-12, bitscore: 44.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDG
                     DTPAAAAASLSESAPVSEERVHQDVLALVRSLSEADLVEVTQ"
     aSDomain        11815..12063
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_N7E53_RS18650_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.10E-24"
                     /identifier="RREFam002.1"
                     /label="N7E53_RS18650"
                     /locus_tag="N7E53_RS18650"
                     /protein_end="85"
                     /protein_start="2"
                     /score="76.4"
                     /tool="antismash"
                     /translation="LSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDGDT
                     PAAAAASLSESAPVSEERVHQDVLALVRSLSEADLVEV"
     gene            12069..12494
                     /locus_tag="N7E53_RS18655"
     CDS             12069..12494
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469533.1"
                     /locus_tag="N7E53_RS18655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_037640859.1"
                     /sec_met_domain="PF13471 (E-value: 9.1e-25, bitscore: 85.5,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQATRLPLHRQIAPRCAAGAARLLVGLPPARLHRVLRV
                     LSKGSRPAGYAQVARARRSVVSVSTRCAGLGCLQRSVATVLLLRVRGRWADWCTGFRVQ
                     PFAAHAWVEADGRPVDEPGEVGVFRTVLAVRRTGGGS"
     gene            12491..13456
                     /locus_tag="N7E53_RS18660"
     CDS             12491..13456
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056405.1"
                     /locus_tag="N7E53_RS18660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_037640860.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTTPAVNGLDLTVPEGATFGFLGPNGAGKTTT
                     ISMLTTLLRPTAGRAEVAGFDVTTHAAEVRRRIGIVFQESTLDLELTATENLRFQADLC
                     GLGRAEARAAVAAMLDMMELTGRDRTPVRHFSTGLRRRLEIARGLLGSPRVLFLDEPTT
                     GLDTQTRAAVWHHLDRLREEQGITVFFTTHQLEEAEHCDRIAIFDSGKLVTEGSPAELK
                     SVIGADVVDLRTEDDRLAVDLLSDRFGLTAESVPGGLRLRVQDGASTVPRLCTGLGLTV
                     RSVTVTPPSLDDVYLHHTGTAIRDSGSDARSLDSLGEGLR"
     gene            13453..14322
                     /locus_tag="N7E53_RS18665"
     CDS             13453..14322
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123205.1"
                     /locus_tag="N7E53_RS18665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_037640862.1"
                     /transl_table=11
                     /translation="MTRTDIPPSVTLTKPAPSSSEARTWHTVRPYALLWRREMTRLRHN
                     PLRLVMGLVTPLLFLVVLGTGLEAASSTLGKAQLNDYRAYLFPGALVMSVQAPAIAVGI
                     SLVWDRRLGMLRQMLVSPFPRSSIVLGLALGGATTGAVYGLALLAVGGIAGVRYTPMLL
                     VVLVELLLVSLLFTSFGLLAAVTIRQVDTFQIVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPDVLTSEQTRLMLGDWNPPVYAELGVLAALTAAVLSLA
                     TYRFSRTQ"
     gene            14336..14809
                     /locus_tag="N7E53_RS18670"
     CDS             14336..14809
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.5"
                     /locus_tag="N7E53_RS18670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_078492945.1"
                     /transl_table=11
                     /translation="MTFVRTWAGTAARIVLAAVLGYAGWVKVQDLTEAGRTVALYQFVP
                     EESAQLVGAAVAFVELALALLLLAGLATRAVAAVTALLMVTYIAAIASVWARGMSIDCG
                     CFSSGGTLTGGAERGYVVDIARDLAFLGAAAFLITRPRTRYALDRWVLETKER"
     gene            14812..15537
                     /locus_tag="N7E53_RS18675"
     CDS             14812..15537
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.5"
                     /locus_tag="N7E53_RS18675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_037640865.1"
                     /transl_table=11
                     /translation="MQPSTDAQVREMVHRRRRRRRTVLVSLAAVVTVLGAALVGAGLVR
                     ATNTEPGEAPEEVPAGVAADRAGLVTSEGPVRVDLYLDYLCPECRITEKALEPKLRKMQ
                     ERGEVRVVHHPVAFLDDRSAPAGYSTRAASAAACAADQNKFQPYTAALFDEQPPEQGPG
                     LDTDRLVALGRDVGITGASFERCVRDGTYRPWVTYVSDVAASRGVALTPTVKVAGKRVE
                     LSGPDRAKAFVRAVEESRS"
     gene            15534..16856
                     /gene="mslH"
                     /locus_tag="N7E53_RS18680"
     CDS             15534..16856
                     /codon_start=1
                     /gene="mslH"
                     /inference="COORDINATES: protein motif:HMM:NF038314.1"
                     /locus_tag="N7E53_RS18680"
                     /note="Involved in the biosynthesis of MS-271 and related
                     lasso peptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="lasso peptide C-terminal Trp epimerase"
                     /protein_id="WP_037640867.1"
                     /transl_table=11
                     /translation="MSRLTVALTGDCMTTRGAPLTSDPAAGRLGEVLHTADFAFTNLEV
                     APATGRGHPVPDAASGGGLIADPAVLDDVTAMGFSALSCANNHALDLGTEGVLGTADLL
                     RAKGVPFAGIGADLTAARRPVYVDRPAGSLALIACTATFRDGQEAAVPSPDLPGRPGVN
                     PLRHGVTLRVTAAQLEALREIDGVTGLRSRRAEARTLLGFDPAMPGPGRLALFGTRFET
                     ADTPGLTTACDPRDVHDITRWVAEARRRADLVLVSVHSHEPGPTPETPAEFLRDFARAV
                     IDAGADAVVGHGPHMLRGAELHRGRPVFHSLGNIVSQIELADRVPAEDYARAGTDEALT
                     PGAYFERITAHGRRMFAPHRRYWQSLVPVLTFDDGVFTGARLHPVDLGFGQPVHRRGRP
                     RLAGPVDAKEILTDFARLAEPYGVTVTVGDDGTGELVAAET"
     gene            16956..17633
                     /locus_tag="N7E53_RS18685"
     CDS             16956..17633
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942992.1"
                     /locus_tag="N7E53_RS18685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_033274901.1"
                     /transl_table=11
                     /translation="MAEAVRVLIADDQALLRGSFRVLVDSTPGMETVGEAGDGAEAVAL
                     ARELTPDVVLMDLRMPVMDGIAATREICSDAALAGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDASPDDLVTGIRVIATGEGVLAPSVTRRVIANFSHQDTAARPAPRLVGLTRREQ
                     EVLVLIAKGLSNAEIADHLVISLPTVKTHVSSLLSKLNARDRAQLVIVAYESGIAERGM
                     PPA"
     misc_feature    complement(16957..16971)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            complement(17835..18977)
                     /locus_tag="N7E53_RS18690"
     CDS             complement(17835..18977)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024753.5"
                     /locus_tag="N7E53_RS18690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_352378638.1"
                     /transl_table=11
                     /translation="MLAVGGTAAWWNWRTDGDDPFDIPPAVAAPKARALDAAKGDYVLG
                     EAPEPLWQAPSALSDRPAAPLPVRDVLVVAAAKGGIAAHSVVDGSVRWSAPDVEATGRY
                     LSLSDRLVAAVDREGTLVTFVASTGEPKWTAPARAASLLDADAEAVYLITEDGRIRAVN
                     RSDATVRWTVPIDADLGEKAASRGLAARRRLVVTAADGSVAALDTADGRQVWQHRSLTD
                     SGRADVDRSGGTLCVAGRQLAAYDLADGKELWSTETPDQPSGDPAFWSSPTVDGRTVYA
                     TEVVFPVRLDLRTGRPTDWIHTGLLQCDAGSPLVLQGSGLWSVATDGEQAAVQALDLSQ
                     EQRPTWTFPVLRNRDRYWITGDANRVFLVDGTSLSALPVF"
     misc_feature    19150..19165
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.6"
                     /tool="antismash"
     misc_feature    19151..19165
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 20.56"
                     /tool="antismash"
     gene            complement(<19206..19929)
                     /locus_tag="N7E53_RS33740"
                     /pseudo=""
     CDS             complement(<19206..19929)
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012298.5"
                     /locus_tag="N7E53_RS33740"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="serine/threonine-protein kinase"
                     /pseudo=""
                     /sec_met_domain="Pkinase (E-value: 4e-10, bitscore: 38.1,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPLESGDPIRLGPYRVHGVLGEGGMGKVYVGRDGEGRVAAVKVL
                     HPHLAHDAGLARRFVREAQMARAVTSATVARVLDARTEDGRPWIATEFLAGPTLADVVG
                     SHGPLDEPTVRALAAGLARAQGHPLRGARPPRREARQHRAHLDGPPRHRLRHRPPRTRP
                     HPDHHRPGPGHPRLRRPRAGPRPARRPARRRLLPGRRPRVRGERSARVRGGARRRRAVR
                     GRPRSAPPGPGTPLSPAAD"
     gene            complement(19953..20219)
                     /locus_tag="N7E53_RS18695"
     CDS             complement(19953..20219)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N7E53_RS18695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_193454478.1"
                     /transl_table=11
                     /translation="MVALDSANGARRWAQTGQAVTAGDLGTPPVAGIAFVYGKVPSGLM
                     TADLRTGAVSHTVKAAAARYFAHRSAKGLIAVGDAYTAAYPLA"
     gene            complement(20159..21001)
                     /locus_tag="N7E53_RS18700"
     CDS             complement(20159..21001)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019334.5"
                     /locus_tag="N7E53_RS18700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_261675384.1"
                     /sec_met_domain="Pkinase (E-value: 3e-33, bitscore: 113.9,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFSPLTHDDPHSVGPYRLVARLGSGGMGTVYLARTATGRTAALKT
                     MHARIATDPAFRTRFRLEADAARVIGPVHGARVFDADPQADTPWLATEYVIGPPLDDAV
                     ALAGPLPEPTVRGLGALLCAALGQLHASEVVHRDLKPSNIMITADGPKVIDFGIARALG
                     DDRLTRTGMAAGTPAFMSPEQATGQEHTSAGDVFALAGVLTYAATGHGPFGTGQHADLV
                     YRVRYAEPDLTGVPAALVPVLTRCFAKDVWCTRWRRARAWSRSTARTAPGGGRRPVRR"
     gene            complement(21008..21907)
                     /locus_tag="N7E53_RS18705"
     CDS             complement(21008..21907)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009190984.1"
                     /locus_tag="N7E53_RS18705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine/threonine protein kinase"
                     /protein_id="WP_193454480.1"
                     /transl_table=11
                     /translation="MERPRHDDPAWAGPHRILARLEREPDHAVAERRFIARSADGDRTL
                     LVRLPVPGADPARWAVEAEGARRLATPGFLPVEEVGGTEDLPWCATPYIPAVSLPAALR
                     AHGGPLPDPTVRALATALAGTLAMAHTRGVTHAGLSPASVLLTTEGPRLSCFGAARAAA
                     PDGELRSDLPGLDPGSLAPEQAAGGRPRLLGDVYALGAVLAHASTGHTAPESRELPEFL
                     RVVIPACLSRDPARRPQAHEVLALLDPDVPGQAAAHPPTAPDTASPLPLPAAVVAALAR
                     QSAQVLAAELPSPPAALD"
     misc_feature    complement(22137..22154)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.41"
                     /tool="antismash"
ORIGIN
        1 ctgctggtcg tcgccggcgc gtccgtggcc ggggccgtgg cggccgccgt cgccgtggcc
       61 gtgctgcacg ccaaggacct cggcggcggg cccgtcctgt tcggtctgac ggtgggcgcg
      121 ctcaccggcg gcgtggtcgt cggcatccgt acggcgcccg ccctgctgcc gtcgctgtcg
      181 cggcggcgca tgctcgccct caccctggcc ttcacgggcc tcgcgctgct cgccgccggg
      241 ctcgtcccgg acgtcaccag cgtgctgctg atcctggcgc tcgccggcgt cggcgcgggc
      301 atggccgcga acatcgcgca caccctgctc gaccaggaga ccgaggagca gcgccggcct
      361 cgggtcaccg agcacctgca cgcggtcgtc cgcgtgtacg tcgggctcgg cgcggtgatc
      421 gcccccgtcg tggcggcggc catcggcccg caccgcctgg agaacggcaa gttcgtcttc
      481 gcccacggcg gcgccgcctt cacgctcatg ctcgtcggcg ccctgctgct gccggtcgcc
      541 gcgatggtgc tggccaaggt cgacgaccgc tccgggatcc cgctgcggca ggacctgcgg
      601 gacgcgctgc tcggcggcga cgaccccgcc cccgcctccg caggcaccgg cttcttcatc
      661 gccctggagg gcggcgacgg cgcgggcaag tccactcagg ccgaggccct cgccgactgg
      721 atccgctcca agggccacga ggtcgtcctc acccgcgagc ccggggcgac ccccgtcggc
      781 aagcggctgc gctcgatcct gctggacgtc tccagcgccg gcctctcgca ccgcgccgag
      841 gccctgctct acgcggcgga ccgcgcggag cacgtggaca ccgtcgtgcg cccggcgctg
      901 gagcgcggcg cggtcgtcat cagcgaccgg tacatcgact cctccgtcgc ctaccagggc
      961 gccggacgcg acctctcgcc caccgagatc gcccgcatca accgctgggc caccgccgga
     1021 ctcgtgccgc acctgacggt gctgctcgac gtgtcgccgg agaccgcccg ggagcggttc
     1081 accgaggcgc cggaccggct ggagtccgag cccgcggagt tccacgcgcg cgtgcgctcc
     1141 ggcttcctga ccctggcggc cgccgacccc ggccgctacc tggtggtcga cgcgggccag
     1201 gagccggagg ccgtcaccac ggtgatccgg caccggctcg accgcgtcct gccgctgtcc
     1261 gaggccgaga tcgccgcccg cgaggaggcg cgccgcaagg ccgaggagga ggcccgtcgc
     1321 aaggcggagg aggaggcggc tcgcaaggcc gaggaggagc gcctggagcg ggagcgccag
     1381 gagcagctcg ccaagctgcg cgccgaggag gaggagcgca agcggcgcga gctggaggag
     1441 gcgcagcggc gcgaggccga gcgccaggcg gaggaggccc ggcagcgtgc cgaggaggcc
     1501 cgccgccgcg cggaggagga gcgtcagcgg ctgctcgccg aggagaaggc ccgcgccgag
     1561 gaggaggcgc gccgcaaggc cgaggaggag cgccgccgca agcaggcgga ggaagaggcg
     1621 cggctgcgcg ccgaggccga ggcgcagcgc ctggagaagc agcgcaaggc cgaggaggcc
     1681 ctgctgcgcg ccgagcaggc ccggcagctc gccgagcgcg cggccgccgc ggcggaggcg
     1741 ggcccgcgca ccgcggcgac cccctccgag gccccggccc ggcccgcctc gggccaggac
     1801 gccgacgagg cgctgaccgt gccgacgccg gtggtcacgc cgaccaacgc gaccggcggc
     1861 cccgtggacg agacggccgt tctgccgcgc gtccgggacg aggaggccgc gcgggacgcg
     1921 gaccgtccgg cgcctgccga cgccgaggtg acgaccgagc tgccccggcc gcaggtgccg
     1981 ggcgcggccg acgagacggc ggtgctgcct cccgtcccgc cgggcgcggc cgacgagacg
     2041 gcggtgctgc ccccggtgcg cgaggacggg cccggtcccg cgggcggcgc cgaggaaccc
     2101 gaggaccgta cgcgtgagct gccgcaggtc gacgccggga acgccccgcg ccgccgggag
     2161 cgtcccgact gggcggagga gaccccgctg gacgacctgc cgtcgctggc cgacgaactg
     2221 ctcggcccgc acgaggacga ccacggcgac gacgacggcc gtggcggcgg acggtggcgc
     2281 cggcgctgaa tcgcatgttg tgccgtgccg ggcgtgcctt cgggcgcgcc cggcaccgct
     2341 gtgtccgggg atcgtggctc agggatgtcg gtccggaccc gcacaatgga tccggcacag
     2401 tacgaacgcg aacgcgacgg aagggcgggc tgacccatga ccgtgtggga cgacctggtc
     2461 gggcaggagc gggtgagcga gcagctcgcg gccgccgccc gggacgccga cgcgttcgtc
     2521 acggccgcgg cgagcgacgc gcccccgccc gaggcgtccc gcatgacgca cgcgtggctg
     2581 ttcaccggcc cgcccggcgc gggccgcaac caggcggcgc gcgccttcgc cgccgccctc
     2641 cagtgcgtca gccccgaccg cgccctcggc ggcgcccccg gctgcggctt ctgcgacggc
     2701 tgccacaccg cgctcgtcgg cacccacgcc gacgtctcca cggtggccgc cgtcggcgcg
     2761 gagatcctgg tcaaggacat gcgcgacacg gtccgcaagt cgttcaccgc cccggcgaac
     2821 ggccgctggc aggtgatcct cgtcgaggac gccgagcggc tcaacgagaa gtccgccaac
     2881 gccgtcctca aggccgtcga ggaacccgcc ccgcgcacgg tgtggctgct gtgcgccccg
     2941 tccgtcgagg acgtcctgcc gacgatccgc tcccgctgcc gccacctcgt cctgagcacg
     3001 ccgtcggtgg aggccgtcgc cgacatgctc gtccgccgcg agggcatcga gccggacgtc
     3061 gccgcctccg tcgcccgcgc cacccagggc cacgtcgacc gggcgcgccg cctggccacc
     3121 gatccggccg cgcgcgcccg ccgtgccacc gtgctgaaac tgccgcagcg ggtcgaggac
     3181 gtcggcggcg cgctgagggc cgcccaggag ctggtcgacg cggccgccga ggacgccaag
     3241 cagctcgccg aggagatgga cggcaaggag accgaggacc tgaaggcggc gctgggggcg
     3301 gcccagggcg gccggctccc gcggggcacg gcgggcgtgg tcaaggatct ggaggacatg
     3361 cagaagcggc gcaggacgcg cacccagcgc gacagcctcg acgtcgccct cgccgacctc
     3421 accagcttct accgcgacgt cctcgcgctg cagctcggct cgcgcatcgc cctcgccaac
     3481 accgacgccg aggacgcgct ggagcgtctg gcccgcagca gcacgcccga gtccacgctg
     3541 cgccgtatcg aggcgatcgc ggcctgcggc gaggcgctgg accggaacgt ggcgccgctg
     3601 ctggcggtgg aggcgatgac gatggcgctc agggcgggat gacgctcagg gcgggatgac
     3661 gctcagggcg ggctgagcgg ggcgtcgtcg cgcggtggcg taactcgtcc ggggcacggc
     3721 gtgtacgcag agcttccgtg ctgtcgctca ccgttacgct cccggtatgc acacacggcg
     3781 caccccccgc aggacccgta ccgcagcagc cctgttcacc gcagccgcgc tgttgtcggc
     3841 gggctgctcc tccggcacct cgacgaacac gacggccagt tcaccggtgg cgggagcggc
     3901 cgccgccctc gccccactgc ccggctcgac ccccgcggcc ctcgccgagt actacgcaca
     3961 ggaaccggcg tggcgcgact gcggcgtccc cgggttccag tgcgccacgg tcaaggcgcc
     4021 gctcgactac gccgaaccgt ccgcagggga cgtccgcctc gccgtcgccc gcaagaaggc
     4081 caccggcccc ggcaagcggc tgggctccct gctggtcaac ccgggcggac ccggcggctc
     4141 ggcggtcgac tacctccaga tgtacgcggg catcggctac cccgaggagg tgcgcgcccg
     4201 ctacgacatg gtggccgtcg acccgcgcgg cgtcgcccgc agcgaacccg tcgagtgcct
     4261 ggacggacgc gcgatggacg cgtacacccg cacggacacc acgcccgacg acgagaagga
     4321 gaccgagacc ctcgtctcgg cgtaccggac gttcgccgag gggtgcgggg cgaaggcgcc
     4381 gaagctgctg cgccatgtgt cgaccgtcga ggcggcccgg gacatggacg tcgtgcgggc
     4441 ggtgctgggc gacgagaagc tgagttacgt cggcgcgtcg tacggcacgc tgctgggcgc
     4501 gacgtacgcg gggctgttcc cggagcggac ggggcggctg gtgctggacg gcgcgatgga
     4561 cccgtcgctg agcgcgcgcc gcctcaacct ggagcagacg gagggcttcg ccaccgcgtt
     4621 cacggcgttc gcgaaggact gcgtgcggcg cagtgactgc ccgctgggcg gcaagggcac
     4681 gagcccggag aaggtcggcg agaacctgaa ggcgttcttc gagaagctcg acgcccgccc
     4741 gctgccggcc ggggacgccg acggccgcga gctgaccgag tcgctggcga cgatgggcgt
     4801 gatcgccgcg atgtacgacg aggcggcctg gcagcggctg cgcgaggcgc tgacgtcggc
     4861 gatgaaggag aacgacggcg ccgggctgct ggtcctctcc gacagctact acgagcggga
     4921 cgcgggcggg gcgtactcca acctgatgtc cgccaacgcc gccgtcaact gcctcgacct
     4981 gccgcccgcc ttcgagaccc cggagcaggt gcgggaggcg ctccccgact tcgagaaggc
     5041 gtccccggtg ttcggcgagg gcctcgcctg ggcctccttg aactgcgcgt actggccggt
     5101 gcgggccacg ggtgaggccc agcgcatcga ggcgaagggc gcggcgccga tcgtggtcgt
     5161 cggcaccacc cgggacccgg cgaccccgta ccgctgggcg gagggcctgg ccggccagct
     5221 ctcctcggcc cgcctcctca cctacgacgg cgacggccac accgcgtacg gccgcggcag
     5281 ctcctgcgtc gactccgcga tcaacacgta cctgatcgag ggcacccctc cggcggacgg
     5341 aaagcgctgc tcgtaaccgg tgcggccacc cccggaggcg acggccccgc cccgcctccg
     5401 ggggcggtac gaagcacccc cggaaactgt gtagacttac cgacgtcgct gatcgcacca
     5461 tggtgcacac ggcacgccgc cttagctcag atggccagag caacgcactc gtaatgcgta
     5521 ggtctcgggt tcgaatcccg aaggcggctc ttcttcaagc cccgcgcaga cagtgtctga
     5581 gcggggcttc ttcgttccgg tgcgttcacg cgctgttccg gaccagttcc ccggcgggct
     5641 cgccgaagcc cgcgtcccgg gccatcacca ccgccgtcgc ccggtcgtgc acctcgagct
     5701 tggtgaagat gcgcgagacg tagttgcgca cggtcttctc ggccaggaag agccggcggg
     5761 cgatctgccg gttccccatg ccgccggcca ggagctccag tatctccagc tcgcggctgg
     5821 tcagccccgg gaacccgacg ctgtcgttca ggccctgcat cgcggagaag tagcggctca
     5881 tgcgggccgc gacgctgggg cagaaggcgg cgccgccccg ggcgacgacc ttcagggagc
     5941 ggagcagttc gtcggccgaa ccgaggcgcg agaggtagcc gttgacgccc gcccggaggg
     6001 ccgagacgat catctggtcg tcgtcgttct gcgccaccac gatcgtgtgc ggccgggacg
     6061 cctcgtcggg gaaggcggag gcgatgtgcc ggacgcgtgc ggagacggtt ctgctggagc
     6121 acggttcgct gaggacgacc acgtcggggt ggagggcggt gccggccagg tccgacagac
     6181 atcggaggtc gtccgagtcg tccgtggcac ggccgacgag agcgcactcc tcatcctgat
     6241 cgacaatcgc ttccagcccg gcgagggaca ccgggttgtc gctgaccacg aacaggttga
     6301 cggtcatctc gtacatcccc cagaaatgcg tcggcgtggt gcggacggcg ccggacggtc
     6361 ccccgggggc acacgcgacg aagggccggt ccgtgtgcga ccgactccgg gcgtgatgca
     6421 gtcagttgtg gcgtgcggtg gcccccgtgg ccatgacctg tcggcgcagg catggtgtgc
     6481 gtcggagcgc gcagggcgct cgtgatgctg agtatgtgga cgcgtgcgga gagaacgcaa
     6541 ggtcccccat gcccccgcac cgtccgaggg gtgagtatcg gccggacccc gccgaaccac
     6601 cgcgttcccg gcgtcggcgg tggtgggggc ggctccgggc gcgcgccccg caggaccgcc
     6661 cgcccgggtg ggccgcggtg cggggcggac gacactggcc gaaacggtca ccgcgcggcg
     6721 cgacacgcgg acgcccggcc ctgcgcccgt tggacgggcc gtggagcgag cgcctacgtt
     6781 cgacggcatg ggtgaggggt cccggaggcg tgcgatcgtg ctggacgggg ctgcggcctg
     6841 cgcgttcacg ctcctgccgc aggtgtcgct gctgcggccg gccgtcgacg gcgggacggg
     6901 cggcggcgtc ctgtggctgc tgtccctggg cgccggactc ccgctcgtcg tacggcggtt
     6961 gtggcccgtc ccggtcttcg tcctcgtcct ggccgccgcc tgcggggcgc tggccgcggg
     7021 cctcggaccg gccgcgttcc tggccgctgc ctacgccgtc tacacggtgg ccaccacccg
     7081 gcggcgcgac cccggggtgt cggccgtcgc cgtcgccggg ctgtgcgcgg cggtcgcggc
     7141 cctgctcacg ctgtcgggcg ggcagtccca ccagggcggc agcaccgccg tgcaggccgt
     7201 gttcggcctc ctcgtgctcg gcgcgacctg ggcggccggc tcggcggtca gggagcgcag
     7261 acgcagtacc cgacgtgcca tcgagcacgc cgccgagcag gcgaagaccg aggagcgcct
     7321 gcgtatcgcc cgcgacctgc acgacgtcgt cacccacagc gtgggactca tcgccgtcaa
     7381 ggccggcatc gccaaccacg tcgtggccac ccgtcccgaa gaggcgaagg aggctctggc
     7441 ggtcatcgag gacgtcagcc gccgggcgct gcgcgacatg cgcgccaccc tgaccgtgct
     7501 gcgcggtgag gaccggagcg acgccgggga cctgcggccc gcccgcggcc tggcggacct
     7561 gcccgcgctg ctcgggaccg ccgaggcggc gggcgtccgt gtcgaactgc gcacccgcta
     7621 cgaccaggag ccgcccgagg gtgtcgcgct ggcggcgttc cgcatcgtcc aggaggccct
     7681 caccaacgtg gtcaagcacg ccgcgccgac cggctgccgg gtggacgtca cggcgcggga
     7741 gggcgtgctg acggtggagg tgaccgacga cggccccgcc cccggacgcc ggaccacggt
     7801 gcccggcggc ggcatggggc tggtcggcat gaaggagcgg accgccgcgc acggcggcac
     7861 cctcagggcc ggcccccggc ccggcggcgg cttccgggtg acggccaccc tgccgttcca
     7921 ggtctgacag cccgacggcc accgactcat cctcgggtat gaggagcgcc cgcgcggcac
     7981 cgcgccgggt tcccgaccgc ggaccgatgc ggtcgcgggg tgcccgccgc gagactcgac
     8041 gtgtgatgct tgccgagaga ctgaccaagc ggtacgggcc cgccaccgtc gtcgacgacc
     8101 tgtccttcac ggtgcgacca ggggtcgtca ccggcttcct cgggcccaac ggcgccggga
     8161 agtccacgac gatgcggatg atgctcggcc tgacccgccc cgacagcggc acggccaccg
     8221 tcggtgtacg cccctaccgc gacctgacct acccgctccg gcacgtcggc gcgctcctgg
     8281 agacgtcggc cccgcaccgt ggcatgaccg ccgtcggcca tctgtcgtgg ctggcgcgca
     8341 gcaaccgcat cccccgccgg cgggtggacg aggtgctcga cgcggtcggt ctgacgggag
     8401 ccgcccgtca gcgggtcggc accttctccc tgggcatggg acagcgtctc ggcctggcgg
     8461 cggccctcct cggcgacccc ccggtcctgg tgctcgacga gccggtcaac gggctcgacg
     8521 ccgagggcat ccggtggctg cgcgaactgc tgcgcgccaa ggccgccgag ggccgaaccg
     8581 tcctcgtctc cagccatctg atggcggaga tggcgcaggt cgccgacgag ctgatcgtga
     8641 tcagccgggg ccgcctcctc gccgagacca gtgtgtcgga gttcatcgga cgccatggcc
     8701 ggacgttcgt acgggtcagg acccccgaac cgctgcgggc cgcgcaggag ttccaggcac
     8761 agggcgccgc ctcggtgcgg cgggccgcgg acggcggcct ggaggtggac ggcctgccgg
     8821 cgggcgaggt gaaccgcatc gccgcggcgg ccggcgtccc ggtcgaggaa ctgagcaccc
     8881 acaccggctc gctggaggag accttcctca agctcgtcga cgacggagga gatcccacgc
     8941 atgtctgaga cgctcgcggc cgctcgggcg gagttcacca agatgcgggc gatgcgcgcc
     9001 acgtccgtcg cgctgctgct gttcgtcgcc gtcagcgtgt tcatcgccgc gctgggcggc
     9061 tggtccgcca agggcgcgat cgagtccgac aaccccgggc tgcgctccga cttcacgccc
     9121 gagcaggccg gcctggacgg catcctctac ggccagctcg ccctgatcgt gttcggggtg
     9181 ctgatgatgt ccggcgagta cacgtcgggc atgatgcggg tctcgctgct cgcggtgccc
     9241 cggcgcggcc ggctctacct ggccaagacg gccgtcaccg ccgccgcggc cctggccgtc
     9301 gccctcccgg tcacggtcgt gtcgtacctg gtcacccaac tcgccctggg ccctcacggg
     9361 tccacgctcg acgcggacgg cgtcccgcgc gccctggccg gcggggtcgt ctacctcacc
     9421 ctcatgagcc tgttcgccgt aggggtggcg gccgccgccc gcagtgccgt cctcccgctg
     9481 gccgtgctgc tgcctctggt gctggtcggc tcgcagatcc tgtccgtcat cggggcgacc
     9541 aaggaggtgg cgcgctggtt ccccgaccgg gccggcgccc agatgctcac cgtcgactcc
     9601 ggtgacgccg tcaccggcct cgtcgtgctg ctcgcgtgga ccgcggccgc gctgacggcc
     9661 ggctgggtgc ggcatcgctc gtgggacggg tgacgggacg ggcctgagtc atgtgcccca
     9721 tgcacgtggg acatggtccc tcgtaggttc ccggacaggt cacggagccg aaaaggtcgg
     9781 cccgggccgt tcgacccacg ggaggaacca tgaccgcgat ctacgagccg cccgccctgc
     9841 aggagatcgg cgacttcgac gagctcacca agtgcctcgg catcgggagc tgcaacgact
     9901 tcgccggctg cggttacgcc gtcgtctgct tctggtgatc gcaccggtgc cggtgtgccc
     9961 ctcgcggcac accggcaccg cccgggggga gtgaggcgac atggaattca cagtgcttcc
    10021 ggactgtccc gccggcgccg cgctggcgga ccgggtggcg gcaccgaagc ggatcgacca
    10081 cgcgtcggga cggccctgga tcgtggggga ctgggccgtg ggcgaggcga cggtgaccga
    10141 ggccggcatc cgccgcctgg cggtgttcgg acacacccgg cccgacgacg cgggaacggt
    10201 ctccgcgctc ggccggatgc gctcgctgca cgacgtcgac cgggtcgcgt cccggctgcc
    10261 cggggtcttc cacctcgtgg cctccctgga cggcgcggtc cgcctccagg gctccgtggc
    10321 aggcgtacgg caggtcttca ccacccgggt cgacggggtg acggtcgccg cgagcgccgt
    10381 ggacccgctg ctgcgcctca ccggcgcggg actcgacgag accctgctgg ccgcccggct
    10441 gctggccccc ggcggggcgc cctggccgct ctcgctgcgc ccggtccgcc gcggcgtgga
    10501 cgcgctgccc accggccact ggctggagct cggcgccgac ggacgggccc gcagcgtgcg
    10561 gtggtgggaa ctgcccgagg cgaccctctc gctggaggag ggcgccgggg ccgtccgctc
    10621 cgcgctcacc gacgcgctcg cgacccgcgt cgccccggac cgcacgatca gcgcggacct
    10681 ctccggcgga ctcgactcga ccaccctgtg cttcctcgcc gacgcggccg gcgccgacct
    10741 ggtcacctac cacgtcatgc cgctcgacga ggccaacgag gacaccgcat gggcccgcaa
    10801 ggccgccgcg catctgccgc acgcccgcca ccacatgctc gccgcggacc gcgccgccaa
    10861 cctgttcgac gtcggctaca ccgccgacac cgccgacgcg gcgcccgagg gtccctcgac
    10921 gtgggcctcg ggcctcgcgc acatccggga cctcgccggg cgggccaccg ccgagggcgc
    10981 ggccctgcac ctgtccgggt tcggcgggga cgaactcttc gggcggatgc ccgcctgcgc
    11041 ctggtccctc gcccgcatcc gtccggccga cggcctgcgg ctggtgaacc gctaccggct
    11101 ggccaaccgc tggccgtggc gcagcaccct gcggcagctc gcggaccgtt cgacgttcgc
    11161 gcagaacctg attgaggtcg ccgggcggat caccgccccg cccccgccgg tcaacgagcc
    11221 tgacttcggc tgggtgttcg cgccgcgcat gcccgcctgg gccaccccgg acgcggtggc
    11281 caccgtgcgg gacctgctga ccacggcagc cgaacggacc ccggaaccgc tggacgccga
    11341 ccgggcccgg caccaggctc tgtcctccgt cgtgttcgag gggaacaccg tccggcaggt
    11401 caacaccgcc gtcgccggca ccgggctggt ctgggaggcg ccgttcctcg acgaccgggt
    11461 tctcgaggcg gccttcgcca cccggatcga cgagcggctg gccgccggac ggttcaagcc
    11521 gctgctgacc accgccgtgc ggggtctcgt gcccgacgac ttcctcgccc gccgcgacaa
    11581 gggcgagttc agcgccgaga cgttccgggg catcgaacgc aaccgggacc ggatcctgga
    11641 tctctgcgag gactcgctcc tggcccggct cggcctcgtc gacccggtcg ccttccggtc
    11701 cgcggtgctc aaccccgggc cgatgtccca tcacctccag ccgatccaga ccacggtggc
    11761 gtgcgagagc tggctgcggg cgcaccccca ggactccgga gagaaccgat gaaactgagc
    11821 cttgcccgcg acgtcaccct cacgcccgtc gattccgggg cggtcctgct cgacggccgc
    11881 cgcggccgct actaccagct gaacgcctcc ggctccgcga tcctgcacaa gttgctcgac
    11941 ggcgacacgc ccgccgcggc cgccgcgagc ctgtcggagt ccgcccccgt cagcgaggag
    12001 cgggtgcacc aggacgtgct ggccctggtc cgctcgctga gcgaggccga cctcgtggag
    12061 gtgacgcagt gaccacgccc gccgtcgccg aacaggccac gcgcctcccg ctgcaccggc
    12121 agatcgcccc gagatgcgcg gccggcgccg cccgcctgct ggtcggactg cccccggccc
    12181 ggctgcaccg ggtgctgcgc gtgctgagca agggctcccg gcccgccggg tacgcccagg
    12241 tggcacgggc ccggcggtcc gtcgtctcgg tcagcacccg ttgcgcgggc ctcggctgcc
    12301 ttcagcggtc cgtcgccacc gtgctgctgc tccgtgtccg gggcaggtgg gccgactggt
    12361 gcaccggctt ccgggtgcag cccttcgccg cgcatgcctg ggtcgaggcc gacggccgcc
    12421 cggtcgacga gcccggagag gtcggcgtgt tccgcaccgt gctggccgtg cgccgcaccg
    12481 gaggcggctc atgacggcga tccgggccga gggcctctac gcgtactacg gcaccacacc
    12541 ggccgtgaac ggcctcgacc tgaccgtccc cgagggggcc accttcgggt tcctggggcc
    12601 gaacggcgcc ggcaagacca ccaccatcag catgctgacc acgctgctca ggcccacggc
    12661 gggccgcgcg gaggtggccg ggttcgacgt caccacgcac gccgccgagg tgcggcggag
    12721 gatcggcatc gtcttccagg aatcgacgct cgacctggag ctcacggcca ccgagaatct
    12781 gcgcttccag gccgacctgt gcggtctggg ccgggccgag gcacgcgccg cggtggccgc
    12841 gatgctcgac atgatggagc tcacgggacg cgacaggacc cccgtacggc acttctccac
    12901 cggtcttcgc cggcgcctgg agatcgcccg cggcctgctc ggctccccgc gcgtgctctt
    12961 cctcgacgag ccgaccaccg gcctcgacac gcagacccgc gcggccgtct ggcaccacct
    13021 ggaccggctc cgcgaggagc agggcatcac ggtcttcttc accacgcacc agctggagga
    13081 ggccgagcac tgcgaccgca tcgcgatctt cgacagcggc aagctggtca cggagggctc
    13141 accggcggag ctgaagtccg tcatcggggc cgacgtcgtc gacctgcgca ccgaggacga
    13201 ccggctggcc gtggacctgc tgtccgaccg cttcggactg acggccgaga gcgtccccgg
    13261 cgggctgcgg ctgagagtgc aggacggtgc ctccacggtg ccccggctgt gcaccggact
    13321 gggcctgacc gtgcggtcgg tgaccgtcac cccgccctcg ctcgacgacg tctacctgca
    13381 ccacaccggg accgcgatca gggacagcgg gtccgacgcc cgctcgctcg acagcctcgg
    13441 ggagggactg cgatgacccg gaccgacatc ccgccgagtg tcaccctgac gaaaccggcc
    13501 ccgtcctcgt ccgaggcgcg tacctggcac accgtacggc cctacgcgct gctgtggcgg
    13561 cgggagatga cccggctgcg gcacaatccg ctgcgcctcg tcatgggcct ggtcaccccg
    13621 ctgctgttcc tggtcgtgct gggcacgggg ctggaagcgg cgtcgtccac gctcggcaag
    13681 gcccagctga acgactaccg ggcctacctg ttcccgggcg cgctcgtgat gtccgtgcag
    13741 gccccggcca tcgccgtggg catctccctc gtctgggacc gccggctcgg catgctgcgc
    13801 cagatgctcg tctccccgtt cccacggtcg agcatcgtcc tcggcctcgc gctcggcgga
    13861 gccaccacgg gtgccgtcta cggcctcgcc ctgctcgccg tcggaggcat cgcgggcgtc
    13921 cggtacacgc cgatgctgct ggtcgtcctc gtcgaactgc tgctggtctc gctgctcttc
    13981 acctcgttcg gccttctcgc cgcggtcacc atccggcagg tcgacacctt ccagatcgtg
    14041 gtgaacctca gcctcatgcc gctgatgttc ttctccggcg cgatgttccc gcccaacggg
    14101 ctgccgggct ggctcgacac cgtcgtcaag ctgaacccgc tgacctacgg cgtcgacgcc
    14161 gtgcgccgga ccctgcccgg ccccgacgtc ctcacctcgg agcagacccg gctgatgctc
    14221 ggcgactgga acccgcccgt gtacgcggaa ctcggtgtgc tggccgccct caccgccgcc
    14281 gtactgagcc ttgccaccta ccggttctcc cggacgcagt gaaagggggc cggccgtgac
    14341 gttcgtgcgg acatgggcgg gcaccgcggc ccggatcgtc ctcgcggcgg tcctgggcta
    14401 cgcgggctgg gtgaaggtcc aggacctcac ggaggccggc cgtaccgtcg ccctctacca
    14461 gttcgtcccc gaggagagcg cgcaactcgt cggcgcagcg gtggcgttcg tcgagctggc
    14521 gctggccctg ctgcttctcg ccgggctcgc cacccgcgcc gtggcggccg tcaccgccct
    14581 gctgatggtc acgtacatcg cggccatcgc ctcggtgtgg gcgcgcggga tgtccatcga
    14641 ctgcggctgc ttcagcagcg gcggcaccct caccggcggt gcggaacggg gctacgtcgt
    14701 cgacatcgcg cgcgacctcg ccttcctcgg cgccgccgcc ttcctgatca cccgcccgcg
    14761 aacccgttac gccctggacc gctgggtcct ggagacgaag gagcgttaga catgcagccg
    14821 tcgacggacg cccaggtccg ggaaatggtg caccggcgcc ggcggcgccg ccgcaccgtg
    14881 ctggtgtcgc tcgcggcggt ggtcacggtg ctcggcgccg cgctcgtcgg cgccgggctc
    14941 gtccgggcca ccaacaccga gcccggcgag gcccccgagg aggtccccgc cggcgtggcc
    15001 gccgaccggg ccggcctggt cacgtccgag ggcccggtcc gggtcgacct ctacctcgac
    15061 tacctctgcc cggagtgccg catcaccgag aaggccctcg agcccaaact gaggaagatg
    15121 caggaacgcg gagaggtgcg cgtcgtccac cacccggtcg ccttcctcga cgaccgcagc
    15181 gcacccgccg gctactccac ccgggccgcg tccgccgccg cctgcgccgc ggaccagaac
    15241 aagttccagc cctacacggc ggcgctcttc gacgagcagc cgccggagca gggccccggc
    15301 ctcgacaccg accgcctggt cgccctcggc cgggacgtcg gcatcacggg cgcgtccttc
    15361 gagcggtgcg tccgcgacgg cacgtaccgg ccctgggtga cgtacgtgtc cgacgtcgcc
    15421 gcctcccgcg gggtggccct caccccgacc gtgaaggtgg ccggcaagcg cgtggagctc
    15481 tccggacccg accgggcgaa ggcgttcgtg cgggcggtgg aggaaagccg ttcatgagcc
    15541 gcctgaccgt ggccctgacc ggggactgca tgacgacgcg gggcgcgccg ctcacctccg
    15601 accccgccgc cggccggctg ggcgaggtgc tgcacacggc cgacttcgcc ttcaccaacc
    15661 tggaggtcgc gcccgccacc ggacgcggcc acccggtgcc cgacgcggcg agcggaggcg
    15721 gcctgatcgc ggaccccgcc gtcctcgacg acgtgaccgc gatgggtttc tcggccctga
    15781 gctgcgccaa caaccatgcc ctcgacctcg gtacggaggg cgtcctcggc acggcggacc
    15841 tgctgcgggc caagggggtg ccgttcgcgg ggatcggcgc cgacctcacc gctgcccggc
    15901 gcccggtgta cgtggaccgg ccggccggca gtctggcgct gatcgcgtgc accgccacct
    15961 tccgggacgg gcaggaggcc gccgttccgt cccccgacct gccgggccgg ccgggggtca
    16021 acccgctgcg gcacggcgtg acgctccggg tcacggccgc gcagctggag gcgttgcggg
    16081 agatcgacgg cgtcacagga ctgcggtcgc ggcgggccga ggcgcgcacc ctgctcgggt
    16141 tcgaccccgc catgcccgga ccgggccggc tcgcgctgtt cggcacccgc ttcgagaccg
    16201 cggacacgcc gggcctgacc accgcctgcg accctcggga cgtccacgac atcacccgct
    16261 gggtggccga ggcgcgccgc cgcgcggacc tggtcctggt cagcgtccac tcccacgaac
    16321 ccggcccgac acccgagacg cccgccgagt tcctgcggga cttcgcccgc gcggtgatcg
    16381 acgccggcgc cgacgccgtg gtcgggcacg gcccgcacat gctgcgcggt gcggaactgc
    16441 accggggccg gcccgtcttc cacagcctgg gcaacatcgt cagccagata gaactggccg
    16501 accgcgtccc ggccgaggac tacgcgcggg ccggtacgga cgaggccctc acccccggcg
    16561 cctacttcga gcgcatcacc gcccacggcc ggcgcatgtt cgccccgcac cgccggtact
    16621 ggcagtcgct cgtgccggtg ctcaccttcg acgacggcgt cttcaccggc gcccggctgc
    16681 acccggtcga cctgggcttc gggcagcccg tgcaccggcg aggacggccc cggctggccg
    16741 gtccggtcga cgccaaggag atcctcaccg acttcgcgcg gctcgcggag ccctacggcg
    16801 tcaccgtcac cgtcggggac gacgggacgg gggaactggt ggcggcggaa acctgacggc
    16861 ggccgccggc ccggccgata gagtgccgga gagacaggct gacgaggcgt cgcaccgccg
    16921 gccacggcgg cgacccgagg tacgagggat gcggaatggc cgaggcggtc cgggtcctga
    16981 tcgcggacga ccaggcgctg ctacgcggga gcttccgggt cctcgtcgac tcgactcccg
    17041 gcatggagac ggtgggggag gcgggggacg gcgccgaggc cgtcgcgctc gcccgtgaac
    17101 tgaccccgga cgtcgtcctg atggacctgc ggatgcccgt catggacggg atcgccgcca
    17161 ctcgcgagat ctgctccgac gcggccctgg cgggcgtgcg ggtgctggcc ctcacgatgt
    17221 tcgacatgga cgagtacgtc tacccggccc tccaggcggg tgcgagcggc ttcatgctga
    17281 aggacgcctc gccggacgac ctggtcacgg gcatccgcgt catcgccacc ggcgagggcg
    17341 tgctggcccc ctcggtcacg cgccgcgtca tcgcgaactt ctcccaccag gacacggccg
    17401 cccggccggc cccccggctg gtcggcctca cccggcgcga acaggaagtg ctcgtgctga
    17461 tcgccaaggg cctgtcgaac gcggagatcg cggaccacct ggtgatcagc ctgcccacgg
    17521 tgaagaccca cgtgagcagc ctgctctcca agctcaacgc ccgcgaccgc gcccagctgg
    17581 tcatcgtcgc ctacgagagc ggcatcgcgg agcgggggat gccgccggcc tgagcgggtc
    17641 gcgccccgca ggcggtcgct ttgaccgctc cgtcccgcgt ggcagggggt gcggggcacc
    17701 gctgagttct cgccgcctcg tcggtcgcgc gcacgacggc ttcacgaccc aggtgcaggg
    17761 catgcccgcg gccgccgtgc ccttcacgaa gggctggcga cggagcgggt gccgttcccg
    17821 gtcggctccg gtcctcagaa caccggcaag gcactgagcg acgtgccgtc cacgaggaag
    17881 acgcggttgg cgtctcccgt gatccagtag cggtcgcggt tccggagcac gggaaacgtc
    17941 cacgtcgggc gctgttcctg ggagaggtcc agggcctgga ccgctgcctg ttcgccgtcg
    18001 gtggccaccg accacagtcc gctgccctgc agcacgagcg ggctgcccgc gtcgcactgg
    18061 aggagaccgg tgtggatcca gtcggtgggc cggcccgtgc ggaggtcgag ccgcaccggg
    18121 aagacgacct cggtcgcgta cacggtccgg ccgtcgacgg tcggagagct ccagaaggcg
    18181 gggtcgccgc tgggctggtc cggggtctcg gtcgaccaga gttccttgcc gtcggcgagg
    18241 tcgtaggcgg ccagctgcct gccggccacg cacagagtgc cgcccgaccg gtcgacgtcg
    18301 gcccggcccg agtcggtcag cgaccggtgc tgccacacct gccggccgtc ggcggtgtcg
    18361 agggccgcca cggagccgtc cgcggccgtg acgaccagcc tgcgccgcgc ggcgaggccg
    18421 cgtgacgcgg ccttctcacc gaggtccgcg tcgatcggca cggtccagcg gaccgtggcg
    18481 tcggaccggt tcacggcgcg gatccggccg tcctcggtga tcagatacac cgcctcggcg
    18541 tccgcgtcga gcagtgaggc ggcccgcgcg ggcgccgtcc atttgggctc gccggtggag
    18601 gcgacgaagg tgacgagcgt gccttcacgg tccacggccg ccaccagccg gtcggacagg
    18661 gacaggtagc ggcccgtggc ttccacgtcc ggcgccgacc agcggaccga gccgtcgacg
    18721 acgctgtgcg cagcgatgcc gcccttcgcc gccgcgacga cgaggacgtc gcggacggga
    18781 aggggcgccg cggggcggtc ggagagcgcc gacggcgcct gccagagcgg ctcgggagcc
    18841 tcaccgagga cgtagtcgcc cttcgccgcg tccagagcac gggccttcgg ggccgcgacg
    18901 gccggaggga tgtcgaaggg gtcgtcgccg tccgtcctcc agttccacca tgccgcagta
    18961 ccgccgacgg cgagcacgga ggcgccggcg gcgagccccg tcagcagacg gcgtcgggat
    19021 acgggacgcg gtgcgccctc gtccggtacg gccgtcagct cacggacgcg ctgctcccgg
    19081 agtgtgatgt cctgcgcgat cggcccgccg cgccacgcgc ggtccgcgcc cgcgggcgga
    19141 gccgagtact ggacgatctg gtccgggagg ggccgggccg ccgggtcctg ggcgagacac
    19201 ggtccgatca gcggccggag agaggggggt acccggtcca ggcggggcgc tccgtggacg
    19261 acctcgtact gcacggcggc gacgtgcgcc ccctcgtacg cgtgcagacc gctcgccgcg
    19321 tacacgagga cggcgcccag ggagaagacg tcggcgggcg ggccgacgcg ccggccgagg
    19381 acctgctcgg gggcgccgta gccgggggtg accggggcct ggccggtggt ggtcagggtg
    19441 aggccgtgtt cggggcgggc gatgccgaag tcgatgacgc gggggcccgt cgaggtgagc
    19501 acgatgttgg cgggcttcac gtcgcggtgg acgagccccg cggagtggat gtccttgagc
    19561 gcgggcgagc cccgcagcca gggcccggac cgtcggttcg tcgagcggtc cgtgggaccc
    19621 gaccacgtcc gcgagtgtgg ggccggccag gaactcggtg gcgatccagg gacggccgtc
    19681 ctccgtccgg gcgtccagga cacgggccac cgtcgcggac gtgaccgccc gggccatctg
    19741 cgcctcgcgg acgaagcggc gggcaaggcc ggcgtcgtgg gccaggtgcg ggtgcagcac
    19801 cttcacggcg gcgacgcggc cctcgccgtc ccggccgacg tagaccttgc ccatgcctcc
    19861 ctcgcccagt acgccgtgga cgcggtacgg acccagacgg atcgggtctc cggattcgag
    19921 gggcttcatg gtgggtgcgg ctttctgtga gttcaggcga gggggtacgc ggcggtgtac
    19981 gcgtctccga cggctatgag ccccttggcg gaccggtggg cgaagtaccg cgccgcggcg
    20041 gccttgacgg tgtgggacac ggcgccggtc cgcaggtccg ccgtcatcag cccggagggc
    20101 accttgccgt acacgaacgc gattcccgcg acgggcggtg tgccgaggtc gccggccgtc
    20161 accgcctgac cggtctgcgc ccaccgccgg gcgccgttcg cgctgtcgag cgcgaccatg
    20221 ccctggccct cctccatcgc gtacaccaca cgtccttggc gaaacagcgg gtcaggaccg
    20281 ggaccagggc cgcggggacc ccggtgaggt cgggttcggc gtagcggacg cggtagacga
    20341 ggtcggcgtg ctgtccggtg ccgaagggcc cgtgtccggt ggccgcgtac gtgaggaccc
    20401 cggccagtgc gaagacgtca cccgcggagg tgtgctcctg accggtggcc tgttcgggcg
    20461 acatgaaggc gggcgtgccc gcggccatgc cggtgcgggt gagccggtcg tcgcccaggg
    20521 cgcgggcgat gccgaagtcg atgaccttgg gaccgtccgc cgtgatcatg atgttggagg
    20581 gtttgaggtc gcggtggacg acctccgagg cgtgcagctg ccccagcgct gcgcacagca
    20641 gggcgccgag gcctcgcacc gtcggctcgg ggagggggcc ggcgagggcg acggcgtcgt
    20701 cgaggggcgg tccgatgacg tactcggtgg ccagccacgg ggtgtccgcc tgcgggtcgg
    20761 cgtcgaagac gcgggcgccg tgcacgggtc cgatgacccg ggccgcgtcc gcctcgagac
    20821 ggaaacgggt gcggaacgcg gggtcggtgg cgatacgggc gtgcatcgtc ttcagcgccg
    20881 ccgtacggcc cgtggccgtg cgggcgaggt agacggtgcc catgccgccg ctgcccaggc
    20941 gggcgacgag acggtacggg ccgacgctgt gcgggtcgtc gtgggtgagc ggggagaaca
    21001 tcggctgtca gtccagagca gcagggggcg aggggagttc ggcggcgagc acctgcgccg
    21061 actggcgcgc gagcgcggcg accacggccg ccggcagcgg aagcggcgag gccgtgtccg
    21121 gggcggtcgg cggatgcgcc gccgcctggc cggggacgtc cgggtccagg agggcgagga
    21181 cctcgtgcgc ctgtgggcgt cgggcgggat cacgcgacag gcacgccggg atcacgacgc
    21241 ggaggaactc gggcagttca cggctctcgg gcgcggtgtg gccggtgctc gcgtgggcga
    21301 gtaccgcccc gagggcgtac acgtcgccca gcaggcgcgg ccggccaccg gccgcctgct
    21361 caggggcgag gctgccgggg tccaggcccg gcaggtcgct gcgcagttca ccatcggggg
    21421 cggcggcccg tgccgctccg aagcagctga ggcgtgggcc ctcggtggtg aggaggacgg
    21481 aggccgggga gagacccgcg tgcgtgacgc cccgggtgtg ggccatggcg agtgtgccgg
    21541 cgagagcggt ggccagggcg cggactgtgg gatccggaag tggaccgccg tgggcgcgca
    21601 gggccgcggg aagggagacg gccgggatgt acggcgtcgc gcaccagggc aggtcctccg
    21661 taccgccgac ctcctcgacc ggcaggaacc ccggtgtcgc cagccgtcgt gcgccctccg
    21721 cctcgaccgc ccagcgggcc gggtccgcac ctgggaccgg cagacggacg aggagcgtgc
    21781 ggtcaccgtc ggcgctgcgc gcgatgaaac ggcgttcggc gacggcgtgg tccggttccc
    21841 gctcgagccg ggcgaggatg cggtgcggcc cggcccaggc cgggtcgtcg tgacgcggac
    21901 gttccatgcg agctcccccg agtccgtgat gatcgcgaac gagcctactc acgcgtccct
    21961 gagagcccgg ggccggtgtc ggccgtcgct gcggccgaaa agaacgaggc cccggtcgtt
    22021 cacctctggc gaccgggtcc tcgtgaagcg gatccgccgg cggcgacact cccccgagtt
    22081 gcgctcgcgc gcgcaccccc gggccggatc cgatgtagtg atcacatcaa accttgccaa
    22141 cggatggcta acatgaggcc aatggattcc ccctcggtca cccggaaggc agtggtgcgg
    22201 cgtgacacgg cgcggtctgc gcttcggact gctggggacg cccgtcctct acggcggtga
    22261 gccgacctcc ggtgagccgg cgcacggtga gccggcgcac ggcgagccga cccccgcgga
    22321 cgcggcacct ccgcacaccc cctgcacccg caacggcacc cccctcctcc ccgtcaccag
    22381 ccccaaactc cgcaccctcc tcgccgtcct gctgctcgac gccgggcggg tcgtctccgt
    22441 cgaggtgctg aaggacgccc tgtggggcgg ggagccgccc gtctccgcgc tgcc
//
