LOCUS       NZ_CP104864            10399 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1700065
            Orig. end                         :: 1710464
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(50..64)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     misc_feature    301..316
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 24.02"
                     /tool="antismash"
     gene            complement(430..1278)
                     /locus_tag="N7E53_RS07815"
     CDS             complement(430..1278)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="N7E53_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_033275654.1"
                     /transl_table=11
                     /translation="MKTHLGRRTRVLVATTATAGMVLLAACTSTDDGGGGSTTAGGVEL
                     VKAGQLTTCTHLPYPPFQSEIDGKVQGFDVALIDLVADDLGVKQQILDTPFENFKTGAF
                     LNSGECDLAAAGMTITDERKKNVDFSDPYFNATQAVLVAKDSGVGSLADVKSENVKLGA
                     QAQTTGEDYVKGEGFDPVSFESSDAVLNGLRTGQVKAVVIDYPVVQGWLKDKANADAFT
                     VAEQIDTGEEYGFTVKKGNKALQDAVNKALADAKADGTYKKLYEQWIGPYDESAASPAA
                     S"
     gene            1383..2483
                     /locus_tag="N7E53_RS07820"
     CDS             1383..2483
                     /NRPS_PKS="Domain: Aminotran_5 (52-295). E-value: 1.6e-28.
                     Score: 91.6. Matches aSDomain:
                     nrpspksdomains_N7E53_RS07820_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337954.1"
                     /locus_tag="N7E53_RS07820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_058915861.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.8e-21, bitscore:
                     75.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLLLDLPPLSAARFASIEDRVARLLGTGQDVLITQGEALLPLE
                     GAIRAAAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQVRDAFAEH
                     PEIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPHAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEAEGLEAVMARHASAAAATRAGALALGGGIAPYVRDAADAAPVA
                     TTLRAPDGTTASDLVARASAADPGLPLAAGGGALAREMIRVNHYGPDATRGTVRACLAA
                     LATALTDAGVPVDLAAGLAAAERAWA"
     aSDomain        1539..2267
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS07820_Aminotran_5.1"
                     /evalue="1.60E-28"
                     /label="N7E53_RS07820_Aminotran_5.1"
                     /locus_tag="N7E53_RS07820"
                     /protein_end="295"
                     /protein_start="52"
                     /score="91.6"
                     /tool="antismash"
                     /translation="GPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQV
                     RDAFAEHPEIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASVGAEPVLPDA
                     WGVDLCVIGAQKAMGGPAGVSAVSVSERAWARMAANPHAPRRSYLSLLDWKERWIDGGR
                     KALLHAPAQLEMLALEACVERIEAEGLEAVMARHASAAAATRAGALALGGGIAPYVRDA
                     ADAAPVATTLRAPDGTTASDL"
     misc_feature    2937..2954
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    complement(2939..2954)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2949..2964
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3084..3096
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3121..3627
                     /gene="ectA"
                     /locus_tag="N7E53_RS07825"
     CDS             3121..3627
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="N7E53_RS07825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_033275656.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPTVADGAVLWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDERGEAVGFITGYLRPDSPRTLLVWQVAVDEAHRGRGLAAAMLDALAART
                     AAEHGVDTVETTVSPGNTASERLFTAFAERHGALLEREVLFDTPDFPDGPHDPEVLHRI
                     GPFAL"
     gene            3697..4968
                     /gene="ectB"
                     /locus_tag="N7E53_RS07830"
     CDS             3697..4968
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.3e-72.
                     Score: 236.6. Matches aSDomain:
                     nrpspksdomains_N7E53_RS07830_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="N7E53_RS07830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_006132704.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-75, bitscore:
                     253.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNYILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGKVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEESGITPDIVTVSKSISGYGLPMA
                     LTMFKPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRQRGEQVEQAM
                     IAITEENLADVKEYRGRGLVWGLEFHDKERAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRVLARSVRETA"
     aSDomain        3781..4794
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS07830_Aminotran_3.1"
                     /evalue="1.30E-72"
                     /label="N7E53_RS07830_Aminotran_3.1"
                     /locus_tag="N7E53_RS07830"
                     /protein_end="366"
                     /protein_start="28"
                     /score="236.6"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNYILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGKVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALSELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEESGITPDIVTVSKSISGYGLPMALTMFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRQRGEQVEQAMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5399
                     /locus_tag="N7E53_RS07835"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="N7E53_RS07835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_004932953.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.5e-54, bitscore:
                     180.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETDMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLDGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            5406..6302
                     /gene="thpD"
                     /locus_tag="N7E53_RS07840"
     CDS             5406..6302
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="N7E53_RS07840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_033275657.1"
                     /sec_met_domain="PhyH (E-value: 3.1e-39, bitscore: 134.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTRVTDLYPTRGATEVVTPRQDPVVWGSPDTPGPVSAGDLQA
                     LDRDGFLAIDQLIGPDEVEVYKRELDRLVTDPAIRADERSIVEPKSQEIRSVFEVHKIS
                     EVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAA
                     PIRRPEFIGARDFTPVK"
     gene            complement(6399..7451)
                     /locus_tag="N7E53_RS07845"
     CDS             complement(6399..7451)
                     /NRPS_PKS="Domain: Aminotran_5 (52-344). E-value: 2.4e-24.
                     Score: 77.9. Matches aSDomain:
                     nrpspksdomains_N7E53_RS07845_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522166.1"
                     /locus_tag="N7E53_RS07845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_058915862.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-16, bitscore:
                     59.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFTPKNTFLNTASSGLLPARAVAALDAAVRLRAEG
                     RPLDPLFADVETCRAAYARLAGVPAERVAAGATVALFSSLVAASLPPGAEVLTADDDFT
                     SVLNPFHARGDLKVRSAPLEGLADAVRPGTALVAVSAAQSADGRIADLPALREAARSHG
                     ARTYVDFSQAAGWLPVEAGAYDYTVAITFKWLLGGHGAAFLTAPEDFGDLVPVQAGWTA
                     GENPWDSCYGPVAELAHSARRFDTTPALFSWAGLRASLTLFEELGVDAVRAHDLALADR
                     FRAGLASLGHEPLPAPGSPIVSVPGLGRLQPELSRAGIEVSNRAGNLRASFHLYNTAAD
                     VDRLLDALSG"
     aSDomain        complement(6420..7295)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS07845_Aminotran_5.1"
                     /evalue="2.40E-24"
                     /label="N7E53_RS07845_Aminotran_5.1"
                     /locus_tag="N7E53_RS07845"
                     /protein_end="344"
                     /protein_start="52"
                     /score="77.9"
                     /tool="antismash"
                     /translation="ADVETCRAAYARLAGVPAERVAAGATVALFSSLVAASLPPGAEVL
                     TADDDFTSVLNPFHARGDLKVRSAPLEGLADAVRPGTALVAVSAAQSADGRIADLPALR
                     EAARSHGARTYVDFSQAAGWLPVEAGAYDYTVAITFKWLLGGHGAAFLTAPEDFGDLVP
                     VQAGWTAGENPWDSCYGPVAELAHSARRFDTTPALFSWAGLRASLTLFEELGVDAVRAH
                     DLALADRFRAGLASLGHEPLPAPGSPIVSVPGLGRLQPELSRAGIEVSNRAGNLRASFH
                     LYNTAADVDRL"
     gene            complement(7521..8264)
                     /locus_tag="N7E53_RS07850"
     CDS             complement(7521..8264)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132708.1"
                     /locus_tag="N7E53_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_193468665.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALASFPHRDGVEVVHRSFELDP
                     GRAKDDVEPVLTMLTKKYGMSQAQAEAGEDNLGAHAAAEGLPYRTRGRDHGGTFDMHRL
                     LHLAREHGRQERLLDLLYRANFAEERSLFGDDERLVELAGAAGLDPEAARAVLADPEAY
                     AAEVRADEQDAARLGARGVPFFVLDRKYGVSGAQPVDVFTKALTQAWGERSPLTLVDGG
                     TDAEACGPDGCAVPARENPQAGADV"
     gene            complement(8368..8976)
                     /locus_tag="N7E53_RS07855"
     CDS             complement(8368..8976)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="N7E53_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_193468663.1"
                     /transl_table=11
                     /translation="MDLDIPELPFPLRTYGPDGSWSYEDGVLTGWAGPRQDRFVPPTGE
                     ALESAADAPRLLGAPDGDFQLIARVTVGFAGSFDAGVLYVHVGEEAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVDGKAVWLRVSRTGRAFAFHASRDGKQWTFVRLFTLGDEKE
                     TGAALVGFATQSPMGEGCVVTFDHIEYRLHWPPDLRDGS"
     gene            complement(9024..9620)
                     /locus_tag="N7E53_RS07860"
     CDS             complement(9024..9620)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522169.1"
                     /locus_tag="N7E53_RS07860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261674849.1"
                     /transl_table=11
                     /translation="MDATGPWAGRRFTVTQRATLRTDRYLVTGTGPDGSEDRVLAYAEQ
                     RRFSLREQLTFHTDETKSQVAFTVRERTLLDPGEGFDVLDAEGRPIGAFHERFFTSLVR
                     STWVLEQPGAPNAVGRERNRFVAVLRRAWDWLPLDLVPFAWPYHFDFESDGKPVMTVDK
                     KIGLRDRYLVEVTDPGLDLRLAVAQAVALDTFQGR"
     misc_feature    complement(10093..10107)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.5"
                     /tool="antismash"
ORIGIN
        1 ccgccgatga tctccgtgcc ggggaaggcg agcggcacgg cgacgccgat gaagatgaag
       61 atcagcaggg cgggcaggcc gcggaagatc tcgatgtaga tgccggccag ccagcggtag
      121 gggccgacgg acgacagccg catcagcgcg atgagcatgc cgagcgcgag cccgacgccg
      181 aagccggtca ccgtgtagag gacggtgttc ttcagcgcga gcgtgatgac ctcggggaac
      241 atccgctcgg cgatctcgac ctgcgcgaac tggttctgca gccgtcccca gtcggccgtg
      301 acgccgaagg cgaccacggc cgcgacgaac acggcgtact ggatgccgcg cgacagcctg
      361 cgcttctgcc gccgggtcat gcccttccgc ctggggacgg gcgtcttctc cgtcgtgtcg
      421 gccatggggt caggaggcgg ccggggaggc ggcggactcg tcgtacgggc cgatccactg
      481 ctcgtacagc ttcttgtacg tgccgtcggc cttggcgtcg gcgagcgcct tgttcacggc
      541 gtcctggagg gccttgttgc ccttcttcac ggtgaagccg tactcctcgc cggtgtcgat
      601 ctgctcggcg accgtgaagg cgtccgcgtt ggccttgtcc ttcagccagc cctggacgac
      661 ggggtagtcg atgacgacgg ccttgacctg gccggtgcgc aggccgttga ggacggcgtc
      721 ggaggactcg aaggagaccg ggtcgaagcc ctcgcccttg acgtagtcct cgccggtggt
      781 ctgtgcctgg gcgcccagct tgacgttctc cgacttcacg tcggcgaggg agccgacgcc
      841 gctgtccttg gcgacgagga cggcctgggt ggcgttgaag tacgggtcgg agaagtcgac
      901 gttcttcttc cgctcgtcgg tgatggtcat gccggccgcg gccaggtcgc actcgccgga
      961 gttgaggaag gcgccggtct tgaagttctc gaacggcgtg tcgaggatct gctgcttcac
     1021 accgaggtcg tcggcgacca ggtcgatcag ggcgacgtcg aagccctgca ccttgccgtc
     1081 gatctccgac tggaacggcg ggtacggcag gtgggtgcag gtggtgagct gccccgcctt
     1141 gaccagctcg accccgccgg cggtcgtgga cccgccgcct ccgtcgtcgg tggacgtgca
     1201 ggcggcgagg agcaccatcc cggccgtcgc ggtggtggca accaggaccc gggtcctgcg
     1261 gccgagatgt gtcttcacgg aggcctcctg tggggggaac ggaaccgtcc gccgaaggtt
     1321 taccctcgac gtcgtcgtac ctacccgcgc tgccgtgaag catgaacaaa gagagcacag
     1381 ccgtgacgaa cctcctcctg gacctgcccc cgctgagcgc cgcgcgcttc gcctcgatcg
     1441 aggaccgggt ggcccggctg ctgggcaccg ggcaggacgt gctgatcacc cagggcgagg
     1501 cgttgctgcc gctggagggc gcgatccgtg cggcggccgg gcccgggacg accgcgctga
     1561 acgtcatcac cggcccctac gggcagacct tcggcgactg gctccgggac tgcggcgcca
     1621 ccgtcatcga cctgacggtg ccgttccaca cggcggtcac cgccgagcag gtgcgggacg
     1681 cgttcgccga gcacccggag atcgacttcg tgtcgctggt gcacgccgag gccgccaccg
     1741 gcaacaccaa cccggtcgcc gcgatcggcg aggtcgtgcg ggagcacggg gcgctgttct
     1801 acctggacgc ggtggcctcc gtgggcgcgg agccggtgct tccggacgcg tggggcgtgg
     1861 acctgtgcgt catcggcgcg cagaaggcga tgggcgggcc ggccggggtg tcggcggtgt
     1921 cggtgagcga gcgggcctgg gcccgcatgg cggccaaccc gcacgctccg cgtcgctcct
     1981 acctctccct cctcgactgg aaggagcggt ggatcgacgg cggccgcaag gcgctgctgc
     2041 acgctccggc gcaactggag atgctcgcgc tggaggcgtg cgtggagcgg atcgaggcgg
     2101 aggggcttga ggcggtgatg gcgcggcacg cctcggcggc ggccgcgacc cgggcgggcg
     2161 cgctggcgct gggtggcggg atcgcgccgt acgtgcgcga cgcggcggac gcggcgccgg
     2221 tggcgacgac gctgcgggcg ccggacggca cgacggcgtc ggacctggtc gcgcgggcgt
     2281 cggcagccga tcccgggctg ccgctcgcgg cgggtggggg tgctctggcg cgggagatga
     2341 tccgggtcaa ccactacggg ccggacgcca cgcggggcac tgtgcgggcg tgcttggcgg
     2401 ctttggccac ggccctgacg gacgcggggg tgccggtcga cctggccgcg gggctggcgg
     2461 cggcggagcg ggcctgggcg taggcggggc ggggccccgc ggccctgggc gagcttctcg
     2521 ggcgccggtg ccggtgcgcg acgccctccc ccgctcccgg ctgcgcccct cggggccgcg
     2581 ggggtgcggc tacgggcgga cggctggggc ggtgcgcccc gccaggggcg cggggaactg
     2641 cgcgatcacg ccccgcgggc cgcggccggt ggacggcagg acacggcata cgcggcgtca
     2701 ccacgctcag ggcctgggcc ccgccaaggc gcgccgatgt ccgcagggac aggggactgc
     2761 ggagtgaatt ccaggggatt atcggaatct ctcacagaaa cactcacgcg atattctccg
     2821 gcttatttcc ctattactct gcagaacagc ttcccgtctt cttctgcccg gtctccggga
     2881 gcctaaacgc cccaatccgg ggagcggcta ccggacgttt ccgggcgcgg tcacgagagt
     2941 tacacagatg tgaccggttc cacatcgcgt ccgctttcca ccctttcctc ccgtgtgaaa
     3001 cgggacattt caccggcacc tcgcgcggac ccacccgcac gcgcgataac acatgacgcg
     3061 ctcgtacgta accccgcagg acgatgcaat tttgaattta cctcggtaag ttcaattcgc
     3121 atgactgccg cacaagcaga cctgcaaatc gaccgcccga ccgtggcgga cggagccgtg
     3181 ctgtggcgga tagccaggga ctcgaaggtc ctcgacctga actcgtcgta cagctatctg
     3241 ctgtggtgcc gtgacttcgc cgccacctcg gccgtcgctc gcgacgagcg cggagaggcg
     3301 gtcggcttca tcaccggcta cctccgcccg gacagcccgc gcaccctgct ggtctggcag
     3361 gtcgccgtcg acgaggcgca ccgcggccgc ggtctggccg ccgccatgct cgacgcgctg
     3421 gccgcccgga ccgccgcgga gcacggcgtg gacaccgtgg agaccaccgt ctctcccggc
     3481 aacaccgcct ccgagcgcct gttcaccgcg ttcgcggagc gccacggcgc cctgctggag
     3541 cgcgaggtgc tcttcgacac cccggacttc cccgacggac cgcacgaccc cgaagtcctg
     3601 caccgcatcg gaccgttcgc gctctgacgc gccggcccga cccccccgat cttccgactc
     3661 cctctgactt cccacgccac cgaggagcga ttcgtcgtga ccatcaccca gcccgacctc
     3721 agcgtcttcg agaccctcga gtccgaggtg cgcagctact gccgcggctg gcccaccgtc
     3781 ttcgaccgtg cgcacggcag ccgcatgtac gacgaggacg gccacgagta cctcgacttc
     3841 ttcgccggag ccggttcact caactacggc cacaacaacc ccgtcctgaa acgcgccctg
     3901 atcgactacc tggagcggga cggcgtcacg cacggcctcg acatgtcgac gacggccaag
     3961 cgcgcgttcc tggagtcctt ccagaactac atcctgcgtc cgcgtgacct gccgtacaag
     4021 gtcatgttcc cgggcccgac gggcaccaac gccgtggagt ccgcgctgaa gctggcgcgg
     4081 aaggtgaagg gccgcgagtc cgtcgtgtcg ttcaccaacg ccttccacgg catgtcgctg
     4141 ggctcactcg ccgtgaccgg caacgccttc aagcgggccg gcgccggcat cccgctggtg
     4201 cacggcacgc cgatgccgtt cgacaactac ttcgacggca aggtcccgga cttcctgtgg
     4261 ttcgagcggc tgctcgagga ccagggctcc ggcctcaaca agcccgccgc cgtgatcgtc
     4321 gagaccgtgc agggcgaggg cggcatcaac gtggcccggc ccgagtggct gcgcgccctg
     4381 tccgagctgt gcgagcgcca ggacatgctg ctcatcgtcg acgacatcca gatgggctgc
     4441 ggccgcaccg gcgcgttctt ctccttcgag gagtcgggca tcacgccgga catcgtcacc
     4501 gtctccaagt cgatcagcgg ctacggcctg cccatggcgc tgaccatgtt caagccggag
     4561 ctggacgtct gggagccggg cgagcacaac ggcacgttcc gcggcaacaa ccccgcgttc
     4621 gtcacggcca ccgccgccct ggaggcgtac tgggcggacg gctccgcgat ggagaagcag
     4681 acccgccagc gcggcgagca ggtggagcag gcgatgatcg ccatcaccga ggagaacctc
     4741 gccgacgtca aggagtaccg cggccgcggc ctcgtgtggg gcctggagtt ccacgacaag
     4801 gagcgcgccg gccgggtcgc caagcgcgcc ttcgagctcg gtctgctgat cgagacgtcc
     4861 ggcccggaga gcgaggtcgt caagctgctc cccgccctga ccatcacccc ggacgagctg
     4921 gacgagggcc tcagggtcct ggcccgctcc gtccgcgaaa ccgcctgaaa cacatccagc
     4981 cgaggaggca tcgcaacacc gtgatcgtcc gttcgttcaa ggagatcgaa ggcaccgacc
     5041 ggcacgtgaa atcggcgtcc ggcacgtggg agagcaagcg catcgtcctc gccaaggaga
     5101 aggtcggctt ctccgtgcac gagacgatcc tgtacgcggg tacggaaacc gacatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgca ccaagggcga cgccgagctc accgaccgcg
     5221 agaccgggaa gacctaccac atcacgcccg gcaccatgta cctcctcgac ggccatgagc
     5281 ggcacacgct caaggtcaag gaggacttcc actgcatctg tgtcttcaac ccgcccgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctcaccgag gaggtgtgat
     5401 ccaccatgac cacgaccacg cgcgtcaccg atctctaccc gacccgcggt gccaccgagg
     5461 tggtcacccc gcgtcaggac ccggtcgtct ggggttcccc ggacacgccg ggccccgtct
     5521 cggcaggtga cctccaggcg ctggaccgcg acggcttcct ggccatcgac cagctgatcg
     5581 gcccggacga ggtcgaggtc tacaagcgcg agctggaccg gctggtgacc gacccggcga
     5641 tccgcgcgga cgagcgctcg atcgtcgagc cgaagtccca ggagatccgc tcggtcttcg
     5701 aggtgcacaa gatcagcgag gtcttcgcga agctggtgcg cgacgagcgg gtcgtcgggc
     5761 gcgcccggca gatcctcggc tcggacgtgt acgtccacca gtcgcggatc aacgtcaagc
     5821 ccggcttcgg cgcgagcggc ttctactggc actcggactt cgagacctgg cacgccgagg
     5881 acggcctgcc gaacatgcgc accatctcgg tctcgatcgc gctcaccgag aactacgaca
     5941 ccaacggcgg gctgatgatc atgcccggct cccacaagac cttcctcggg tgtgcgggag
     6001 agacgccgaa ggacaactac aagaagtcgc tccagatgca ggacgcgggg acgccgtccg
     6061 acgaggcgct gaccaagatg gcgtcggagt acggcatcaa gctgttcacc ggcaaggccg
     6121 gctcggcgac ctggttcgac tgcaactgca tgcacggctc cggcgacaac atcacgccgt
     6181 acccgcgcag caacgtgttc atcgtgttca acagcgtcga gaacacggcg gtcgagccct
     6241 tcgcggcccc gatccgccgc cccgagttca tcggcgcgcg ggacttcacc cccgtgaagt
     6301 agggacacgc acggcggtga cgtgtgcgca ccgcctcacc gactgcgggc cgggcctcct
     6361 cgtgtgggac aaggaggcac cggcccgctg ccgtgcgctc agccggacag cgcgtcgagc
     6421 agccggtcca cgtcggcggc cgtgttgtag aggtggaacg acgcccgcag gttgccggca
     6481 cggttcgaca cctcgatgcc cgccctcgac agctccggct ggagccgccc cagccccggc
     6541 acggacacga tgggcgagcc gggcgcgggc agcggctcgt ggcccagcga ggcgagtccg
     6601 gcgcggaagc ggtcggcgag ggcgaggtcg tgggcgcgga cggcgtcgac tccgagttcc
     6661 tcgaagagtg tgagggacgc ccgcaggccc gcccagctga acagggcggg ggtggtgtcg
     6721 aacctccgtg cgctgtgggc gagttcggcg acgggcccgt agcagctgtc ccacgggttc
     6781 tccccggccg tccagccggc ctgcaccggg accaggtcgc cgaagtcctc gggggcggtc
     6841 aggaacgccg cgccgtgccc gcccagcagc cacttgaagg tgatggcgac ggtgtagtcg
     6901 tacgcgccgg cctccaccgg cagccagccc gccgcctggg agaagtcgac gtaggtgcgg
     6961 gccccgtggg agcgggccgc ctcacgcagc gcgggcaggt cggcgatccg gccgtcggcg
     7021 gactgcgcgg cgctgaccgc gaccagcgcg gtgcccggcc gcacggcgtc ggccagccct
     7081 tccagcggcg cggaccgcac cttgaggtca ccgcgcgcgt ggaaggggtt cagcaccgag
     7141 gtgaagtcgt cgtcggcggt gaggacttcg gcgcccggcg gcagggacgc ggcgaccagt
     7201 gaggagaaca gggcgacggt cgccccggcc gccacccgct cggcgggcac gcccgccagc
     7261 cgggcgtacg cggcacggca cgtctcgacg tcggcgaaca gcgggtcgag cgggcgcccc
     7321 tccgcgcgca gccggacggc cgcgtcgagc gcggccacgg cccgggcggg cagcaggcca
     7381 ctgctcgcgg tgttcaggaa ggtgttcttc ggggtgaact cggcacggac gaggctctcg
     7441 aaggtctcca tgggaccact ctgcgacccg tccgtgcccc cgtccatcgc acatgtctgt
     7501 gcggtaccgc taaggaacgc ttatacgtcc gcgccggcct gcgggttctc ccgcgcgggc
     7561 accgcgcacc cgtccggacc gcaggcctcg gcgtccgtgc ccccgtcgac gagggtcagc
     7621 ggggaccgct cgccccaggc ctgggtgagc gccttggtga agacgtccac gggctgggcg
     7681 ccggagacgc cgtacttgcg gtcgagcacg aagaacggca ccccgcgcgc gccgagccgg
     7741 gcggcgtcct gctcgtcggc gcggacctcg gcggcgtacg cctccgggtc ggcgagcacc
     7801 gcgcgggcgg cctcggggtc gaggccggcg gcgccggcga gttcgacgag ccgctcgtcg
     7861 tcaccgaaca gggagcgctc ctcggcgaag ttggcccggt agagcaggtc gagcagccgc
     7921 tcctggcggc cgtgctcgcg ggcgaggtgc agcaggcggt gcatgtcgaa ggtgccgccg
     7981 tggtcgcggc cccgggtgcg gtagggcagg ccctcggctg cggcatgggc gcccaggttg
     8041 tcctcgccgg cctcggcctg tgcctggctc atgccgtact tcttggtgag catcgtgagg
     8101 acgggttcga cgtcgtcctt ggcgcggccc gggtcgagct cgaaggagcg gtggaccacc
     8161 tcgacgccgt cccggtgcgg gaaggaggcc agcgccttct cgaagcgggc ttttccgaca
     8221 tagcaccagg ggcaggcgat gtcgctccag atctcgacgc gcatgggcgg gctctctcct
     8281 gggtcgtccg gtcgcggaga cctcctccgc aaccggatga acgttcaagc atccgccgcc
     8341 attcccggtg gttcctccgg gcctcgctca gctgccgtcg cgcaggtccg gcggccagtg
     8401 gagccggtac tcgatgtggt cgaaggtcac cacgcacccc tcccccatcg gtgactgggt
     8461 cgcgaacccg acgagggccg cccccgtctc cttctcgtcg ccgagggtga acagccggac
     8521 gaaggtccac tgcttgccgt cgcgcgaggc gtggaaggcg aaggcgcgcc cggtgcggct
     8581 cacccgcagc cagaccgcct tgccgtccac ggtgaaggag ttggcgtcgt cggagtggcc
     8641 ccgggtgacg accgtgcaga cggtgggcac gtccggggag tactccaggc acagcttggc
     8701 ccacgcctcc tcgccgacgt gcacgtagag caccccggcg tcgaaggagc cggcgaagcc
     8761 gaccgtgacc cgggcgatca gctggaagtc gccgtcgggc gcgccgagca gccggggcgc
     8821 gtcggcggcg gactccaggg cctcgccggt gggcggcacg aagcggtcct gccggggtcc
     8881 cgcccatccg gtgaggacgc cgtcctcgta ggaccaggag ccgtcggggc cgtaggtccg
     8941 cagcgggaag gggagttcag gtatgtccag gtccatggcg ggattgtccc aggagcggcc
     9001 ccggcgcgtg tgccggatcc cggtcagcgg ccctggaagg tgtccagggc gaccgcctgg
     9061 gcgacggcga gccgcaggtc gaggccgggg tcggtcacct cgacgaggta gcggtcacgc
     9121 aggccgatct tcttgtcgac ggtcatcacg ggcttgccgt cgctctcgaa gtcgaagtgg
     9181 tacggccagg cgaacggcac caggtccagc ggcagccagt cccaggcccg gcgcagcacg
     9241 gccacgaacc ggttgcgctc ccgccccacg gcgttgggcg cgcccggctg ctccagcacc
     9301 caggtggagc ggaccaggga ggtgaagaac cgctcgtgga aggcgccgat cggcctgccc
     9361 tcggcgtcca gcacgtcgaa gccctcgccg gggtccagga gagtgcgttc cctgacggtg
     9421 aacgcgacct ggctcttcgt ctcgtcggtg tggaaggtca gttgctccct gagggagaac
     9481 cgcctctgct cggcgtaggc gaggacccgg tcctcggagc cgtccggccc ggtcccggtg
     9541 acgaggtacc ggtcggtcct cagggtggcc ctctgcgtga cggtgaagcg ccggccggcc
     9601 cagggtcccg ttgcgtccac tgctgtgctc ccggtgtcgg tggagggtgc ggagccggca
     9661 caggtcctcg ccggctccgc catcgtcgcg gggcgggccg tgccggcgca gtctccggaa
     9721 gtcgcggtcc gcaacgacca acggcggtac ggacgttgac gggcgccgtt cactttcgtc
     9781 gttcggggcc gctaccgagg gaggatgccg gcgccccggt gcgggcccct agggtcgggc
     9841 gcgtggacac agcgccgatc tctcgccccg cccgcctctc gctgtcccgg ctggccgccg
     9901 cctccccctg gtcgcgcggc cggatcgccg gggagattgt gctgctcctg gtgctcgcgg
     9961 gcatcgcggg cccgatgtac gccgtggaga gcggatccgc cgcgaagggc tgggcggtgg
    10021 ccctggtcgt cgcgctgctt tccctggtcc ggcggtcgct gcccgccacc gtgctcgtcg
    10081 tctcctccgc gctggccggc tcgatcctcg gcaccggccc gctgctcatc tacgcgagct
    10141 ggtcggcggg cagccgcgtc aagcgcccct ggcgcgcggt cggcgcgtac gccgccggtc
    10201 tggtgctgta cgtcgcgggt ggtctgctcg gggacggggc cgaggacgca ctggagctgt
    10261 cgctgctgat ggcggcggcc ctgggcgcgg gcccctacct ggtgctggcg gtcgtgccgg
    10321 ggctcgcctc ccggtaccgc gcgcaacgcc gtgacctgct gtgggccctg cgccggcaca
    10381 acgcccaact ggtgcgcga
//
