LOCUS       NZ_CP104864            11380 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 158260
            Orig. end                         :: 169640
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..11380
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11380
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11380
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6380](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6380)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(333..2126)
                     /locus_tag="N7E53_RS00575"
     CDS             complement(333..2126)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613010.1"
                     /locus_tag="N7E53_RS00575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_193454551.1"
                     /transl_table=11
                     /translation="MSTVTVPTAAGADLPARGALRALLPALAAHRAMTARTCAAALLEQ
                     GSLVALVTLAAHTVGIAVIEGRAPTAGTVTALVALVLVRALMTWREMDLSHDLAYRVLA
                     ALRVHVFDGMARSAPARVAGRRSGDLAATAMADVEALEFFYAHTTAQLLAAGTVFTGGS
                     VLLILVEPWLLVAVLPVAALLAAAPFADAGARTVRGGRTRTAVAALSADTVETVDGLRE
                     LLAFGALAERRRTLAERGRQVGGAQRAEATWEAVAAAVRDLLIVVAVLGVVAAAAQSVT
                     SGRLHGAWVPAAMALALSVLGPVAESARALSQAVALRTAAARVDAAVKAPALAPPPTAP
                     RPLPSGPLGVRLHRVRFDYGGTPVLDGVDLTVRPGRTLALVGASGAGKSTCAHLLARFW
                     DPSAGEVQLLPADGDPVDVREVNDAELRRAVVLVGQDTPLFHGTLAENLRLAAPEADDR
                     ALAEAARLCGVDRIAPLDTLVGERGATLSGGQRARIALARALLAGPRVLVLDETTAHLD
                     NTGDAELATALRQESRTTIFIAHRPATIRRADRIAVLEDGLITEEGTWEDLTSSPDSAL
                     NRILSDTALTGHGSSPREPDQ"
     gene            complement(2123..3862)
                     /locus_tag="N7E53_RS00580"
     CDS             complement(2123..3862)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004120.1"
                     /locus_tag="N7E53_RS00580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_261674457.1"
                     /transl_table=11
                     /translation="MIVHPELRRAARHARRPLLAAALLQGAVTLTHLAQAVLLALALAG
                     LARGDTGQLPWLLAGVLAVVAARAELALWQRRTATRAGARVRVALRDELLAHLGRLGPA
                     YLTTARAGAVRTTLVDGVEGVDAYVSRYLPQLLVTLAVPPLVLAVLVVVEPRALLGLVP
                     ALLLALLGPRAWDRLLAARGKEHWDTYEGLGADYLEALQGMPALRAAGAVGRTRARLEE
                     RSAALHRATVAKLRVSLVDTGITDLAIQGGTAAAALLACWSAVTGSTTAAGTYLVLLLA
                     SECFRPVRDHAREWHAGYLGVSAADGLASLRTSVPAVPDTATAPAHWHTPPELSFHSVR
                     FTYDGAPAPALDGVSFTARAGRTTAIVGPSGAGKSTLLALLLRHHDPQEGQITLDGRPT
                     TEYALDSLRQGIAVVSQETYLFHATIADNLRLARPDATDDELTRAARAAGIHHEIAALP
                     DGYATLLGERGATLSGGQRQRLALARALLSDAPVLVLDEATSSVDERREADIVRELLNA
                     VGGRTVLVVAHRLAAVRSADRIVVLDAGRVDAVGDHTGLVDAGGVYTRLVEAGRAHRGE
                     LAA"
     gene            complement(3859..4992)
                     /locus_tag="N7E53_RS00585"
     CDS             complement(3859..4992)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048308.1"
                     /locus_tag="N7E53_RS00585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_033274226.1"
                     /transl_table=11
                     /translation="MEDHREDPQTGLVAALARARSPERPGPDTPAGAPVRAWAGPPLPI
                     PQAGPAHLDLRTLLHLSLAASDTSGRLRPTPSAGALHPVDTELVVGTGCSLPPGRYGYD
                     PLRHRVHHVGPPVDGTPPGVTVALSVTAQRTVSHYGHRAWPLLLLDTGHAAAALLLAAR
                     VLGAGESDVRMDGRTENPLAVIRIPPPYGRSPRRPAGTEDPPTPAELLARRSTPPPLVG
                     LPPREALRAVLATAERAGGRGLLRWCAAVGPPRPGLMHLGPEGTALRRYASGEARPTLA
                     AWAAGQAWLADAGAVLLAHGCPADADATHIRRAHLRAGFAVHLAHVTALRHGLAARPVG
                     SWQQADLGAALGAAPGRDWVVHALALGTHHADEESST"
     gene            complement(5001..6380)
                     /locus_tag="N7E53_RS00590"
     CDS             complement(5001..6380)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004118.1"
                     /locus_tag="N7E53_RS00590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_058915320.1"
                     /sec_met_domain="YcaO (E-value: 3.7e-48, bitscore: 163.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALPLEALVDPVCGIVRKVKPVEHPAGAPPRYTALTAEVSDARR
                     HGLWPADRVSLGTTFGDPEQARVAAIAEGMERYCGNRVPPPGHPDAPLRATAAELADKG
                     LRLYGPADLPAYAPWQYARAKFPYRPLTADTPALWTRGTERLPDGTAAEVWAPVSLTHL
                     NWRQGGLRELPRTHHLNYAGIATGQGLDDAVERGLLEIVERDALELWWHLDGPARGIDP
                     ASVPGLADDLAGSALDLHIVEMPSEFAPCMAALVHDPRLGLSAAGFACKYDPAEAARKA
                     VLEAVHTWVFTQGLTDPDGWVFQAVEAGLLARGLYLDHRADGRYLDVCGEQFEHVRDLG
                     AHVQVWLDERMAVEARRFTEPALGTVSVAEVEPGSRERLESTLREGGHRVMTFDLTTED
                     VAETALRVARVLVSGLLPNAPAAFGYFGSPRLAETAVARGWRATAPSAPEDFTLAPPPH
                     M"
     gene            complement(6377..7858)
                     /locus_tag="N7E53_RS00595"
     CDS             complement(6377..7858)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017947325.1"
                     /locus_tag="N7E53_RS00595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_033274228.1"
                     /transl_table=11
                     /translation="MTAFSTAVGELATDVLLPAGHGPFPAVLVRTPYDRTRHEAEARAW
                     ARRGFAAVVQDVRGRFASPGEWHPYAHEAADGTAAVRWIREQPWSDGRLVAAGASYAAH
                     CALALALDAPWDARPDAVIAAVPALGLAETAREASGVERLLSRAGWWAAHGDRRDSDEN
                     ALNKALAEDPGLLAHLPLTALPERLGRALPSWAGLWRRPDRGRLVARAAHAGPPLLAVG
                     GHHDHFTEETVALWRAWGGSSARLLLGPWGHPLTAAPGPDAGPAHRLNLGELYVRWARR
                     ALTGDLAPGHHGALALGGAGLWLPADAEGEPRAHRMRLLHGSAFTADPVRPLRSDDLTV
                     PADGPPDRCLLVTPPSPRPLDLVGLVSVRLRATAHTPSADWAARLVVLPPDGAAERLAV
                     GVVRRAEPAGQETAFTVGLGRLARRLRAGTRLRLEIAGHHFPAHARNPHTGDDPMTAAQ
                     LLASRREVDPDSVVLNLPVLPSRPAPTDPAEEILR"
     gene            complement(7855..8724)
                     /locus_tag="N7E53_RS00600"
     CDS             complement(7855..8724)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048303.1"
                     /locus_tag="N7E53_RS00600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_203551514.1"
                     /transl_table=11
                     /translation="MSRSRLVPGAEVVAVPGRGLAVRTPAGEFFSVRTGDVDEDALLSL
                     LTGGTTAPEDEGMECVIRAFEEAGYLEAGYLAEAPSRPEWPAARQDVRILGDPVLTEPL
                     AVQLAALGARPRTTPAVTDFGSSPVGIEDLLDGHPSAVVWCLDGPVPDGLWDAADRLPE
                     HGVAWLRCHREGWQAYVEPVAAAPGDVTSAHVRSRRLAATPAHRELAAYWSGPRTSGAP
                     VRLAVPAATLLAALLADDLGRWATGASGPAGFPVRRRLRRVDLRTLTVTEHPVLPVPDV
                     APIPKKDG"
     gene            complement(8815..8928)
                     /gene="amiA"
                     /locus_tag="N7E53_RS00605"
     CDS             complement(8815..8928)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004115.1"
                     /locus_tag="N7E53_RS00605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_240449383.1"
                     /transl_table=11
                     /translation="MENEQVFAPIADPGQLAHDSASHSNALVENPFEDTEE"
     misc_feature    8985..8998
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.45"
                     /tool="antismash"
     misc_feature    complement(8985..9000)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.17"
                     /tool="antismash"
     gene            complement(8986..9807)
                     /locus_tag="N7E53_RS00610"
     CDS             complement(8986..9807)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004114.1"
                     /locus_tag="N7E53_RS00610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_203551513.1"
                     /transl_table=11
                     /translation="MNESVGIHVEGVHFGYPGRPVLRGVDLTVRPGELTALIGLNGCGK
                     STLLRLAAGLLKPDEGRVLLGEDDVTRLSRRATARKVALLHQSAPAVPGMTVRHLVRQG
                     RYAARGPLGMLREGDDPVVRRALRDVGVEDWADRDVDALSGGERQRVRLAMALAQDTRV
                     LLLDEPTTYLDLHHQLDVLQTVVRLREERGLTVVMVLHDLAHAARFAERIVALREGRVA
                     ADGAPKQVVTPGLLAEVLQVAGRVGEDPEGGWPVCYPDHPIAALEYENQIH"
     gene            complement(9828..10835)
                     /locus_tag="N7E53_RS00615"
     CDS             complement(9828..10835)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328328.1"
                     /locus_tag="N7E53_RS00615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron ABC transporter permease"
                     /protein_id="WP_037637613.1"
                     /transl_table=11
                     /translation="MTRRFTALGVLAVVCAAVELVAGRGMSPGVVWDVLNGGGDATERH
                     ILFQLRLPRLLVALGAGACLAVAGLVLQSALRNPLAGPEVTGVTPGAVLGAVTATALGL
                     AGWDSPLAVVLAACAGGCAGAALLWLLAGRGRGDPAQTAVHGVLVSAVLGGLTAMVLLV
                     EPGELGSVVQWLVGTTEGRVWEHWHLLWPWALVWGVAAWLLAGPLTLLRCGDDIALAAG
                     LSARRARTLALMCAVALTAGAVAAVGALGFIGLLVPHLAVAVFGADLRRSLPGAALTGA
                     VVVCGADAVAQLSSRLLATALDSGRLTLPVGALTACLGAALLLLVVRRTPSRTF"
ORIGIN
        1 tccgccgcca gtcgccgcag cagggcgctg gaccggttcg ccttgtgcgc gaagtagacc
       61 cgcccggtga gacggtggcg gcggtagacg gcacggaagc gggcggcgtt cgtggccacg
      121 gcctcgggga gcagcacatt gagcggcgac cccagcccgt cggcgagcga gtgcaggaag
      181 ggcccgtccg ccagcagtgc ggcgagcggc ggttccaccc tgggcctcag gtacaagggc
      241 gcgtccacgt gagacccctc ccgttgccgg ccgaccgccg acgcgacggt cgttcgggag
      301 tagtcgtttc cacgtacacc cgtacctaag cgtcactggt ccggttcccg gggcgaactg
      361 ccgtgcccgg tgagggcggt gtcactgagg atgcggttga gcgcgctgtc ggggctgctt
      421 gtgaggtcct cccaggttcc ctcttccgtg atgaggccgt cctccaggac cgcgatgcgg
      481 tcggcacggc ggatggtggc cgggcggtgg gcgatgaaga tcgtcgtgcg gctctcctgc
      541 cgcagcgcgg tggcgagttc ggcgtcgccg gtgttgtcca gatgcgcggt ggtctcgtcg
      601 agcacgagga cgcgcggtcc ggccagcagc gcgcgggcca gggcgatgcg ggcccgctga
      661 ccgccggaga gcgtggctcc ccgttcgccg acgagggtgt ccaggggggc gatccggtcg
      721 acgccgcaca gccgtgccgc ctcggccagc gcccggtcgt cggcctcggg cgcggcgagc
      781 cgcaggttct cggcgagggt gccgtggaag aggggggtgt cctgtcccac caggaccacc
      841 gcccgccgca gttccgcgtc gttcacctcc ctcacgtcca cgggatcgcc gtcggcgggc
      901 agcaactgga cctcgccggc ggacgggtcc cagaagcgtg ccagcaggtg ggcgcaggtg
      961 gacttgccgg cgcccgaggc gccgacgagg gcgagtgtcc ggccggggcg gacggtgagg
     1021 tcgacgccgt ccagcacggg ggtgcccccg tagtcgaacc gcactcggtg caaccggacg
     1081 cccagtggtc ccgaaggcag gggacgtggt gcggtgggcg gtggtgccag tgcgggggcc
     1141 ttgacggccg cgtcgacgcg ggcggcggcg gtgcgcaggg ccacggcctg gctcagggcg
     1201 cgggcggact ccgcgaccgg gccgaggacg gacagggcca gcgccatcgc ggccgggacc
     1261 caggcaccgt gcagccgtcc ggaggtcacg gactgtgccg ccgcggcgac cacgccgagg
     1321 accgcgacca cgatgaggag gtcgcggacg gcggcggcga cggcctccca ggtggcctcg
     1381 gcgcgctggg cgccgccgac ctgccgcccc cgttccgcca acgtgcggcg gcgttcggcc
     1441 agagcgccga aggcaagcag ttcgcgcagc ccgtcgacgg tctccaccgt gtcggccgac
     1501 agcgcggcca cagctgtgcg ggtgcggccg ccgcgcaccg tgcgcgcgcc tgcgtcggcg
     1561 aagggcgccg cggcgagcag ggcggcgacc ggcagcacgg ccaccagcag ccacggctcc
     1621 accaggatca gaagtaccga gccgccggtg aagaccgttc ccgccgcgag cagctgggcg
     1681 gtggtgtggg cgtagaagaa ctccagcgcc tccacgtcgg ccatggcggt ggcggcgaga
     1741 tcgccgctgc gccggcccgc gacccgggcc ggcgcgctgc gggccattcc gtcgaagacg
     1801 tggacccgca gtgccgccag tacccggtag gcgagatcgt gggagaggtc catctcccgc
     1861 caggtcatca gggcgcgtac gagaacgagg gcgacgagcg cggtgacggt gccggccgtg
     1921 ggagcgcgcc cttcgatgac ggcgatgccc acggtgtgcg cggccagggt caccagcgcg
     1981 acgagagaac cctgttcgag gagcgcggcg gcacaggtgc gggccgtcat ggcacggtgt
     2041 gctgcgaggg ccgggagcag ggcgcgcagc gcgccccgcg cgggaaggtc agctccggcg
     2101 gcggtgggaa cggtgacggt gctcatgcgg cgagttcccc tcgatgggcg cggccggcct
     2161 cgacgagccg ggtgtagacg ccgccggcgt cgaccaggcc ggtgtggtcg ccgacggcgt
     2221 ccacccgtcc cgcgtcgagg acgacgatcc ggtcggcgga ccggacggcc gcgagccggt
     2281 gggcaaccac caggacggtc cgcccaccga ccgcattcag cagctctcgg acgatgtccg
     2341 cctcgcgtcg ttcgtcgacc gagctggtgg cctcgtcgag aacgaggacc ggggcgtcgg
     2401 acagcagggc ccgcgcgagg gcgagccgct gtcgctgccc gccggagagc gtggcgcccc
     2461 gttcgccgag gagggtggcg tagccgtccg ggagggcggc gatctcgtgg tggattccgg
     2521 cggcgcgcgc cgcccgggtg agctcgtcgt cggtggcatc cggacgggcc aggcgcaggt
     2581 tgtcggcgat ggtggcgtgg aacaggtacg tctcctggga gacgacagcg atgccctgcc
     2641 gcagcgagtc gagcgcgtac tcggtcgtcg gacggccgtc gagggtgatc tgcccttcct
     2701 gcgggtcgtg gtggcgcagg agcagggcca ggagggtcga cttgcccgcg ccggacggcc
     2761 cgacgatcgc ggtggtccgt cccgcgcgag cggtgaagga gaccccgtcc agagcgggtg
     2821 cgggagcacc gtcgtaggtg aaccggacgc tgtgaaagct gagttcgggc ggggtgtgcc
     2881 agtgcgcggg cgccgtcgcg gtgtcgggga ccgccggcac ggacgtgcgc aaggacgcca
     2941 gcccgtcggc ggccgacacc cccaggtacc cggcgtgcca ctcccgggca tggtcgcgga
     3001 ccggacggaa gcactcggag gccagcagca gcaccagata ggtgccggcc gcggtggtgg
     3061 atccggtgac ggccgaccag catgccagga gcgctgcggc ggcggtgccg ccctggatgg
     3121 cgaggtcggt gatgccggtg tcgaccaggg aaacccgcaa cttggcgacg gtcgcccggt
     3181 gcagggccgc ggaccgctcc tccaggcgcg cccgggtgcg gccgaccgcg cccgcggcgc
     3241 gcagggcggg catgccctgg agagcctcca ggtagtcggc gcccagccct tcgtaggtgt
     3301 cccagtgttc cttcccgcgg gcggcgagga gccggtccca ggcgcgcggg cccagcaggg
     3361 ccaggagcag ggcggggacg aggccgagga gggcccgggg ttcgaccacg acgaggacgg
     3421 cgaggaccag gggtggcacc gcgagggtga ccaggagctg gggcagatag cgggagacgt
     3481 aggcgtcgac gccctccacc ccgtcgacca gggtggtgcg gacggccccc gcccgcgctg
     3541 tggtcaggta tgccggcccc agccggccca ggtgggccag gagttcgtcg cgcagtgcca
     3601 ccctgacccg ggccccggcg cgcgtggcag tgcgccgctg ccacagggcg agctcggcgc
     3661 gggcggcgac gacggcgagc acgccggcca gcagccacgg gagttggccg gtgtcaccgc
     3721 gggccagacc ggcgagcgcg agggccagca gcacggcctg tgccaggtga gtgagggtga
     3781 cggctccctg caagagggcg gccgcgagca ggggccgccg tgcgtgccgt gcggcgcgac
     3841 gcagctcagg gtggacgatc atgtggaact ctcctcgtcg gcgtggtggg tgccgagggc
     3901 cagggcgtgc acgacccagt cacggccagg cgcggcgccg agagcggcgc cgagatccgc
     3961 ctgctgccag gagccgacgg gtcgggccgc cagcccgtgg cgcagggctg tgacatgggc
     4021 caggtggacg gcgaagccgg ctcgcagatg ggcgcggcgg atgtgcgtgg cgtcggcgtc
     4081 cgcggggcag ccgtgggcga gcagtacggc gccggcgtcc gcgagccacg cctggcccgc
     4141 cgcccaggcg gcgagcgtcg gacgggcctc gccggacgcg tagcgccgca gggccgtgcc
     4201 ctcgggaccc aggtgcatga ggccgggccg aggcgggccg accgcggcgc accagcgcaa
     4261 caggccacgg cccccggctc gttcggcggt ggccaggact gcccgcagtg cttcgcgggg
     4321 cggaaggccc accaacggtg gcggggtgct gcggcgcgcc agcagttcgg ccggggtggg
     4381 cgggtcctca gtccccgcag ggcgcctcgg gctccgcccg tagggcggtg ggatgcgtat
     4441 gacggccagg gggttctcgg tgcgtccgtc catccgcacg tcggattcgc ccgcgcccag
     4501 gacgcgggcc gccaggagca acgccgcggc ggcgtgcccg gtgtccagca acaggagggg
     4561 ccaggcccgg tggccgtagt gggacaccgt gcgctgtgcg gtgacggaaa gtgcgacggt
     4621 cacgcccgga ggcgtgccgt cgacgggcgg gccgacgtga tggacgcggt ggcgcagcgg
     4681 gtcgtagccg tagcggccgg gcggcaggga gcagccggtg cccacgacca gttcggtgtc
     4741 caccgggtgc agcgcaccgg cggagggcgt gggcctgagg cgtccggagg tgtccgaggc
     4801 cgccagggaa agatgcagca gagtccgcag gtcgaggtgc gcggggccgg cctgcgggat
     4861 cgggagcggc gggcccgccc aggcccggac gggtgctccc gcgggcgtgt cggggccggg
     4921 gcgctccggg gagcgggccc gggcgagggc ggcgacgagg cccgtctgcg ggtcctcgcg
     4981 gtggtcttcc acagcggccc tcacatgtgc ggcggaggtg ccagcgtgaa atcctccggc
     5041 gcgctcggcg cggtcgcgcg ccatccgcgc gcgacggcgg tctcggcgag gcgcgggctg
     5101 ccgaagtagc cgaaggcggc gggcgcgttg ggaagcagtc cggagacgag gacccgggcg
     5161 acgcgcagtg cggtctccgc cacgtcctcg gtggtgaggt cgaaggtcat gacacggtga
     5221 ccgccctcgc gcagcgtgct ctccagccgc tcccggctgc ccggctcgac ctctgcgacg
     5281 gataccgtcc cgagcgcggg ctcggtgaac ctccgggcct cgaccgccat ccgctcgtcc
     5341 agccacacct gcacgtgtgc gcccaggtcg cgcacgtgct cgaactgctc gccgcacacg
     5401 tcgaggtagc ggccgtcggc ccggtggtcc aggtacagcc cgcgggcgag cagcccggcc
     5461 tcgacggcct ggaacaccca gccgtcgggg tcggtcaggc cctgggtgaa gacccaggtg
     5521 tggacggctt ccaggacggc cttgcgggca gcctcggccg gatcgtactt gcaggcgaac
     5581 ccggcggcgg acagtccgag gcgcgggtcg tggacgagcg cggccatgca gggggcgaac
     5641 tccgagggca tctcgacgat gtggaggtcg agcgcggagc cggcgaggtc gtcggcgagg
     5701 ccgggcacgc tcgcagggtc gatgccccgg gccgggccgt ccaggtgcca ccacagttcc
     5761 agggcgtcgc gttcgacgat ctccagcagg ccgcgctcga cagcgtcgtc gaggccctgt
     5821 ccggtggcga tcccggcgta gttgaggtgg tgggtgcgag gcagctcgcg caggccacct
     5881 tggcgccagt tgaggtgggt gagcgagacg ggtgcccaga cctcggcggc ggtcccgtcg
     5941 ggcagtcgtt cggttccccg tgtccacagc gccggggtgt cggcggtgag cgggcggtag
     6001 gggaacttcg cgcgggcgta ctgccagggt gcgtaggcgg gcagatcggc cgggccgtac
     6061 aggcgcaggc ccttgtccgc cagttcggcg gcggtggcgc gcaggggtgc gtccgggtgg
     6121 ccgggcggcg gcacacggtt gccgcagtag cgctccatcc cctcggctat cgcggcgacg
     6181 cgggcctgct cggggtcgcc gaaggtggtg ccgagggaga cccggtcggc cggccacagg
     6241 ccgtgtctgc gggcgtcgga cacctcggcc gtgagcgcag tgtagcgggg cggggcgccc
     6301 gcgggatgct cgacgggctt gaccttgcgg acgattccgc agacggggtc gacgagcgct
     6361 tccagcggca gagccgtcat cgcaggatct cctcggcggg gtcggtgggg gcgggacggg
     6421 acggaaggac gggcaggttc agcaccacgc tgtcggggtc gacctcacgc cgggaggcga
     6481 gcagttgtgc ggccgtcatc gggtcgtccc cggtgtgggg gttgcgggca tgggccggga
     6541 agtggtgtcc ggcgatctcc aggcgcagcc gggtaccggc ccggagccga cgtgccagcc
     6601 ggccgagtcc gacggtgaac gcggtttcct ggccggccgg ctcggcacgc cggacgacac
     6661 cgaccgcgag ccgctcggcg gccccgtcgg gtgggagcac gacgagcctg gcggcccagt
     6721 cggcggacgg cgtgtgggcg gtggcccgca gccgcacact gacgaggccg acgaggtcga
     6781 gcggacgcgg cgacggcggt gtgaccagca ggcagcggtc gggcgggccg tcggcgggga
     6841 cggtcaggtc gtcggagcga aggggccgga cggggtcggc ggtgaaggcg gagccgtgca
     6901 gcaggcgcat gcggtgggca cgcggttctc cctcggcgtc ggcgggcagc cataggccgg
     6961 cgccgccgag cgccagcgcg ccgtggtggc cgggggcgag gtcgccggtc agggcgcggc
     7021 gggcccagcg cacgtacagc tcgccgaggt tcagccggtg ggcgggtccc gcgtccggcc
     7081 ccggagcagc ggtcagaggg tgcccccagg gcccgagcag cagacgcgcc gaggatccgc
     7141 cccaggcgcg ccacagagcg acggtctcct cggtgaagtg gtcgtggtgg ccgcccaccg
     7201 cgagcagggg cggcccggcg tgcgccgccc gcgccacgag gcgcccgcgg tcgggccggc
     7261 gccacagccc cgcccacgag ggcagggcgc gtcccagccg ctcgggcagg gcggtcaacg
     7321 gcaggtgggc gagcaggccg gggtcctcgg cgagggcctt gttcagcgca ttttcgtcgg
     7381 agtcgcgccg gtcgccgtgg gcggcccacc atccggcgcg ggaaagcagc cgttccacgc
     7441 cggacgcctc gcgggcggtc tccgccaggc cgagggcggg cacggccgcg atgacggcgt
     7501 ccggccgggc gtcccagggc gcgtcaaggg cgagggcgag ggcgcagtgg gcggcgtagg
     7561 aggcgccggc ggccaccagc cgcccgtcgc tccacggctg ctcccggatc cagcggacgg
     7621 ccgctgttcc gtcggcggcc tcgtgcgcgt acgggtgcca ctcccccggc gacgcgaacc
     7681 gcccccgcac gtcctggacg acggcggcga acccgcggcg ggcccaggct cgcgcctcgg
     7741 cctcgtgccg ggtccggtcg tagggggtgc gcaccaggac ggccgggaag ggtccgtggc
     7801 cggccgggag caggacgtcg gtggcgagtt cccccacagc cgtgctgaac gccgtcatcc
     7861 gtccttcttc ggtatcggcg cgacgtcggg caccggcagc acggggtgct cggtgacggt
     7921 cagcgtgcgc aggtccaccc gtcgcagtcg gcgccgtacc gggaagcccg ccgggccgga
     7981 ggctccggtg gcccaccggc cgagatcgtc cgcgagcagc gccgcgagca gcgtggcggc
     8041 gggcacggcg agccggaccg gtgcgccgga ggtacgcggt ccgctccagt acgcggccag
     8101 ttcgcggtga gcgggtgtgg cggcgagcct gcgggagcgg acgtgcgccg aggtgacgtc
     8161 cccgggagcg gcggccaccg gctcgacgta cgcctgccag ccctcgcggt ggcagcgcag
     8221 ccacgcgaca ccgtgctccg gcagccggtc ggcggcgtcc cacaagccgt ccggcacggg
     8281 tccgtccagg caccacacga ccgcggaggg atgtccgtcg agcaggtcct cgatgcccac
     8341 cggcgagctg ccgaagtcgg tcacggccgg ggtcgtacgg ggccgcgcgc ccagggctgc
     8401 cagttgcacg gcgagcggtt cggtcaggac cgggtcgccg aggatcctga catcctgacg
     8461 agcggcgggc cactccgggc gcgacggagc ttccgccaga tagccggctt ccagatagcc
     8521 ggcttcttcg aacgcccgga tcacgcattc catcccctcg tcctccgggg ccgtggtccc
     8581 cccggtgagc agggagagca aggcgtcctc gtcaacgtct cctgttctga cactgaagaa
     8641 ctcccctgcg ggggtgcgga cggcaagccc tcgcccgggc acggcgacga cctcggcacc
     8701 ggggacgagt cggctacggg acacggtgtt cctcctgtcg acgggagcgg ggacgggcgt
     8761 gggcccgccc ggagcatcgg gcgggcccac ggtcgagggt ggccagaccc tcgcttactc
     8821 ctcggtgtcc tcgaaggggt tctcgacgag cgcgttggag tgggaggccg agtcatgagc
     8881 cagctgaccc gggtcggcga tcggcgcgaa aacctgctcg ttttccatga actctccttc
     8941 atcagcaggg agatacggcg ttcggatgag cgccgtgacc tcactctaat gaatctgatt
     9001 ttcatattct agagctgcga tggggtgatc tgggtaacac acaggccacc ccccttccgg
     9061 atcctcaccg acccggcctg ccacctgcag gacctccgcc agcaaccccg gtgtgacgac
     9121 ctgcttcggc gctccgtcgg cggccacacg accctcccgc agggcgacga tccgctcggc
     9181 gaaccgggct gcgtgggcca ggtcgtgcag caccatcacc accgtgagcc cgcgttcctc
     9241 ccgcaggcgc accacggtct gcagtacgtc gagctggtgg tgcagatcca ggtacgtggt
     9301 cggctcgtcg agcagcagga ccctggtgtc ctgagcgagc gccatcgcga gccggacacg
     9361 ttgccgctcg ccgccggaca gcgcgtcgac gtcacggtcg gcccagtcct ccaccccgac
     9421 atcacgcagc gcccggcgca cgacggggtc gtcgccctcg cgcagcatgc ccagcgggcc
     9481 gcgagccgcg taccgtccct gccggaccag gtgacggacc gtcatgcccg ggacggccgg
     9541 agccgactgg tgcagcaagg ccaccttccg ggccgtggcg cgtctggaaa gccgcgtgac
     9601 atcgtcctcg ccgagcagca cgcgcccttc gtcgggtttc agcagcccgg cggccagccg
     9661 cagcagggtc gacttgccgc agccgttcag gccgatcagg gcggtcagtt cgcccggccg
     9721 gacggtcagg tcgacgcccc gcagcacggg tcgtccgggg tagccgaagt gaaccccctc
     9781 gacgtggatc cccacggact cgttcatgct ctgtcctcaa ttcgacgtca gaacgtacgg
     9841 ctcggcgtgc ggcgcacgac gagcagcagc agcgcggcac ccaggcaggc ggtcagcgcg
     9901 ccgaccggca acgtgagccg cccggagtcc agcgccgtcg ccagcagccg cgaggacaac
     9961 tgcgccaccg cgtccgcccc gcacaccacg accgcgccgg tcagggcggc gccgggaagg
    10021 ctccggcgca ggtccgcacc gaagacggcc acggccagat gcgggacgag caacccgatg
    10081 aagcccagcg cgccgacggc agccacggcg ccggcggtca gggccaccgc gcacatcagg
    10141 gccagtgtcc gggcccggcg agcggagaga ccggcggcga gggcgatgtc gtcaccgcag
    10201 cgcagcaggg tcaacggccc cgccagcagc caggcagcga ccccccagac gagcgcccac
    10261 ggccacagca ggtgccagtg ctcccacacc cggccctccg tggtgcccac cagccactgc
    10321 acgacgctgc cgagctcacc cggctccacg agcagcacca tggcggtgag cccgccgagc
    10381 accgccgaca ccagcacccc gtgcacggcg gtctgcgcgg ggtcaccgcg cccgcggccc
    10441 gcgagcagcc acagcagcgc tgctccggcg cacccgcccg cgcaggcggc gaggaccacg
    10501 gccaggggcg aatcccaccc ggcgagcccc agagcggtcg cggtgacggc gccaagaacc
    10561 gcccctgggg tcacgcccgt cacctccggc ccggccaggg ggttacgcag ggcggactgc
    10621 aggaccagtc ccgccacggc cagacaggca cccgcgccga gggccaccag cagcctcggc
    10681 agacgcagtt ggaacaagat gtggcgctcc gtcgcgtcac cgccaccgtt gaggacgtcc
    10741 cagaccaccc ccggggacat gccgcgcccg gccaccaact ccactgcggc gcacacgacg
    10801 gccagcactc cgagggccgt gaaacgccgg gtcaccgtac gtcctccgtt ccgatcgtcc
    10861 cgagaggcgg cgaggtcgga ccaccgggcc cgggttcgcc ccggtgagcg tgatgcgatg
    10921 ccgcggcggg aggtacgtcg tccaccacac ccgaagccgt cctccgcagc tcggacagct
    10981 gctcgcgcgc accgaccgaa gcggcctgtc cgtgccgggc cgtccggcgg cggccacctg
    11041 accgcatcag cgcgacgccg gccggtaccc ccagcagggc cgtccacgcg ccggcggggg
    11101 tctccacggg tgccagcgcc agccgggccg ggacatcggc gaccgccacg acgaccgcgc
    11161 cccaggccgc ggaccagggg agccagcgca ccgctcccgc cacgggagcg aaccagcggc
    11221 tcaggtgcgg ggcgaggaac cccacccacg cgacgggccc gcacacggcc gtcaccgggg
    11281 cgatcagcac gacggcgatg gccagcgctg ccagccgggc gcgctgcgct cgcacaccga
    11341 gcgcctccgc gtcctcgtcg cccagccgca gtacggacag
//
