LOCUS       NZ_CP104864            16614 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Streptomyces lusitanus strain FZ202 chromosome.
ACCESSION   NZ_CP104864
VERSION     NZ_CP104864.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lusitanus
  ORGANISM  Streptomyces lusitanus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Streptomyces lusitanus FZ202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417890)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.8x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025427835.1-RS_2024_10_11
            Annotation Date                   :: 10/11/2024 19:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,707
            CDSs (total)                      :: 6,615
            Genes (coding)                    :: 6,460
            CDSs (with protein)               :: 6,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 83 of 155
            Pseudo Genes (incomplete)         :: 88 of 155
            Pseudo Genes (internal stop)      :: 3 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:00:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 16614
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei,  College of Life
            Science and Technology.
FEATURES             Location/Qualifiers
     region          1..16614
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phenazine"
                     /region_number="1"
                     /rules="phzB"
                     /tool="antismash"
     cand_cluster    1..16614
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="phzB"
                     /kind="single"
                     /product="phenazine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..16614
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[6140:6614](+)"
                     /cutoff="20000"
                     /detection_rule="phzB"
                     /neighbourhood="10000"
                     /product="phenazine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6141..6614
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="phzB"
                     /neighbourhood="10000"
                     /product="phenazine"
                     /protocluster_number="1"
     gene            150..1351
                     /locus_tag="N7E53_RS00005"
     CDS             join(150..441,441..1351)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611170.1"
                     /locus_tag="N7E53_RS00005"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS3 family transposase"
                     /protein_id="WP_261674432.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MVMKNYPPQFKADAVALYRSRPEATIRQVAADLGINPETLRNWVR
                     AAGASRPRGRRAEVPAEPPTPLEAENAALRRKVRELEEEREILRKAAKYFAGGDALVNR
                     FQFVADHHRRYGAKRLCTILGIARSSFYYWRRTAADRAARQAADARLAARIRAVHRESD
                     GTYGVPRITAELREAGERVNHKRIARVMRGAGLAGVRLRRRHRTTVADQAAAKAPDLIG
                     RDFTASKPNTKYVGDITYLPLDGGRFLYLATVIDLASRRLAGWAIADHMRTDLVTDALA
                     AAEQTRGSLAGAVLHTDHGAQYTSRAFADACRKAGVRQPMSTIGSSADNALAESFNATF
                     KRETLQGRKHWSTESEARLDAFRWLHRYNTRRRHSRLGHRSPIAYENTIRTTSTTLAQA
                     A"
     gene            1381..1686
                     /locus_tag="N7E53_RS00010"
     CDS             1381..1686
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N7E53_RS00010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_051724688.1"
                     /transl_table=11
                     /translation="MEAAQRWPDILSLSLHPGVARTDINRDTSLRLFFRYAPFLISPEK
                     SARRLVRLAVTPAEELTSGALYGPRHAIRPKPDIFNADYAARLWATSDAALAEPAT"
     gene            complement(2030..2806)
                     /locus_tag="N7E53_RS00015"
     CDS             complement(2030..2806)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (13-155). E-value: 3.6e-13.
                     Score: 41.7. Matches aSDomain:
                     nrpspksdomains_N7E53_RS00015_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182164.1"
                     /locus_tag="N7E53_RS00015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_058915278.1"
                     /sec_met_domain="adh_short (E-value: 4.5e-31, bitscore:
                     106.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYLLTGRPLDSRVAVVTGASSGMGAATAPRLAELGASVAVIARR
                     QEKLDSLAADITAAGGKALPLSVDVTDRTAVRTAADQVADTLGPADLVFNNAGVQLISA
                     TDELKVEDWQRQIDLNVTGVMNVIAAFLPHLTAGAERGKSADLINTSSIAATRILEKFS
                     IYSGTKAYISHLTRLIRVELGRKNVRVSAIEPGMVDTELPDHVTDPDASALMAGLLHDI
                     ECLRSTDIAETVAFIAALPRHVNISELTILPTAQPV"
     aSDomain        complement(2342..2767)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS00015_PKS_KR.1"
                     /evalue="3.60E-13"
                     /label="N7E53_RS00015_PKS_KR.1"
                     /locus_tag="N7E53_RS00015"
                     /protein_end="155"
                     /protein_start="13"
                     /score="41.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAVVTGASSGMGAATAPRLAELGASVAVIARRQEKLDSLAADITA
                     AGGKALPLSVDVTDRTAVRTAADQVADTLGPADLVFNNAGVQLISATDELKVEDWQRQI
                     DLNVTGVMNVIAAFLPHLTAGAERGKSADLINTSSIAA"
     gene            complement(2848..3687)
                     /locus_tag="N7E53_RS00020"
     CDS             complement(2848..3687)
                     /NRPS_PKS="Domain: PKS_KR (2-153). E-value: 9.5e-10. Score:
                     30.6. Matches aSDomain:
                     nrpspksdomains_N7E53_RS00020_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182165.1"
                     /locus_tag="N7E53_RS00020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_230230052.1"
                     /sec_met_domain="adh_short (E-value: 2.4e-29, bitscore:
                     101.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATWFITGGSRGLGLEIARAALNGGDNVVVGARDPQKLPKELYRH
                     DRVLGVALDVTRSEDITAAVETAVEHFGTINVLVNNAGRGLLGALEEITDAEARSLFDL
                     NVFGLINVTRAVLPVMRAAGAGKIVHIGSRSGFEGEPGVSMYSASKFAVAGISEALAVE
                     LEPFGIQSMVVEPGVFRTDFLDASSLSTAAERIPAYDGTPAHATLDWIDTANHTQLGDP
                     VKGAALIYEVTCQDKLPGHLALGRDAIERQLVKIRSLQEDLAAWQDKSAATACDDAA"
     aSDomain        complement(3229..3681)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N7E53_RS00020_PKS_KR.1"
                     /evalue="9.50E-10"
                     /label="N7E53_RS00020_PKS_KR.1"
                     /locus_tag="N7E53_RS00020"
                     /protein_end="153"
                     /protein_start="2"
                     /score="30.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TWFITGGSRGLGLEIARAALNGGDNVVVGARDPQKLPKELYRHDR
                     VLGVALDVTRSEDITAAVETAVEHFGTINVLVNNAGRGLLGALEEITDAEARSLFDLNV
                     FGLINVTRAVLPVMRAAGAGKIVHIGSRSGFEGEPGVSMYSASKFAV"
     CDS_motif       complement(3601..3681)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N7E53_RS00020_0001"
                     /evalue="5.10E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="N7E53_RS00020"
                     /protein_end="29"
                     /protein_start="2"
                     /score="15.9"
                     /tool="antismash"
                     /translation="TWFITGGSRGLGLEIARAALNGGDNVV"
     gene            3861..4766
                     /locus_tag="N7E53_RS00025"
     CDS             3861..4766
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907406.1"
                     /locus_tag="N7E53_RS00025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_261674433.1"
                     /transl_table=11
                     /translation="MDREHSTSASMAEFLRSARTRLTPREAGLDDPGQGRRRVPGLRRE
                     ELAHLAGVSVDYYTRLEQGRSRSASAEVLDALATALHLDDAERSHLHTLARPRPRQARR
                     RPRPQRVHPATWDLLETLQQAGRPGFLLGRRLDVLAHNPLAGRLITDFRALPAAERNQA
                     RFVFLDPHARELYRDWPRVAADTAAMLRLDAGRHPDDPALSALVGDLSIHSEEFRRFWS
                     DNKVHQRTTGTKDYHHPLVGDLTVTYQALTPADDPDQILFVYGTEPGSPSETSMRVLAQ
                     WSRTAADTGSLTTQTPGAHD"
     misc_feature    5097..5113
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     misc_feature    complement(5111..5124)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.31"
                     /tool="antismash"
     misc_feature    5951..5967
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.0"
                     /tool="antismash"
     misc_feature    6053..6067
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            6141..6614
                     /locus_tag="N7E53_RS00030"
     CDS             6141..6614
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phenazine: phzB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131994.1"
                     /locus_tag="N7E53_RS00030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_058917771.1"
                     /sec_met_domain="phzB (E-value: 8.9e-24, bitscore: 82.6,
                     seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETVSTSQNTDDIRAGAQQAFKNHLEYLSSGRIPEWVDLFTEDG
                     VLEFPYAPEGYPTKTVGKEGLFEYMKNFPQHFRVEFIDLHFHETVDPSLVIAEFKSRGT
                     ALATGRPYNQTYISVVETKGGKISRYVDFWNPLVGMQALGDDPADMVAAFSND"
     misc_feature    complement(6733..6747)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.6"
                     /tool="antismash"
     gene            6740..7285
                     /locus_tag="N7E53_RS00035"
     CDS             6740..7285
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935302.1"
                     /locus_tag="N7E53_RS00035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_058915281.1"
                     /transl_table=11
                     /translation="METRTRQRSTAEERRATVMRTAIKTFAERGYYGTSTMDVAKAAGI
                     SQGYLYRLFKGKETLFAALVDHCSARLLEDAAAAAASVQSARPEEVLKALTASYNETIA
                     DRDVLMILMHAQCAASEPVIGEAVRTCYAKQVEYVRAVSGASNEQLRRYFADGLLSNVM
                     MAIDATTLDTPWARALRA"
     gene            7384..9276
                     /locus_tag="N7E53_RS00040"
     CDS             7384..9276
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="N7E53_RS00040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_261674434.1"
                     /transl_table=11
                     /translation="MPTHSKLRSTGLLLALLAFAQFISAIDYNIVYVALPEIGREVGFD
                     AQNLQWVVSGYAVAFGGFLLLGGRAADLLGHRRMFTTALVLYGLSSLAGGLATDPGLLV
                     AARAVQGLGGALLLPATLSLIGTTFPEGAERNRALAIWGGAGAVGLALGSLLGGVLTNY
                     LGWESVFYVNVPLALGAAAAAFPIITKDGPRERGRSFDLTGALTATVGFSALVFGVVQG
                     PEAGWGSVEALIPLLGGALFTGLFLIVESRSAHPLMPLRLFRNRSLGAAMGVTFVFMGT
                     FGAQYYFFTVYLQNVGGYGTLATGLAFLPSALVGLVGSKLSEKLLGKVGPRTTIVTGLL
                     VGAAGMAVLALGMSPNGGYPALLPGVILLSLGQGIAWAAMFAAATSGIDARHQGIASAM
                     ASTTQQIGSAVGLAILVAIANSGVEVTTGPDLVPGLRTAGFTAAALTLLGVAIALTLRQ
                     PGTTPPAPTATQTTHRPEASISACPPERRAHRPSRRGGQPARREGHLASCTLDAPHSHP
                     QEQDGQGAFDPCDHRRADQGPLLGTGPLPHPHGQRSGTQALRATIRLAQRERPEDRRPR
                     HGHEDRTRWRASPGKCLRRWQFRGGRGDKPREPCPCSPRTVEPRTTPIPKPRLS"
     gene            complement(9312..10418)
                     /locus_tag="N7E53_RS00045"
     CDS             complement(9312..10418)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022404.5"
                     /locus_tag="N7E53_RS00045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2776 family protein"
                     /protein_id="WP_058915283.1"
                     /transl_table=11
                     /translation="MNYGISVLFRAIPVLMGAVSAALGGYVLAHASGASARVAGLVLIF
                     LSAICLCLFSTAATIIRQLIHRYTPTDRIVYPVFGFAISGVAIGYGLSLLTETPRPPPD
                     FIAGHVVFGIGMICVCVSCVATASTRFVQITRNSALPDESPPLSPAPFGPLATRTLLTL
                     PVLASLITWAWAFTLLAGGGGGLAAGRFTAGHVLVGLALVCTCLIGLVASILRQIQNTY
                     THRDRVLWPVSAAVAGAVGIVWALIVLSVHTRPSQLPAAYVMIGLGLICWSILSKVLLL
                     ALVWRREALLANRVPLIPVGTALLCLILSAFLFESADPAVVVAARVLVGLGGICFSLFS
                     IVSILESGTSGESGAHTSGTDGSDLNDT"
     gene            <10595..11307
                     /locus_tag="N7E53_RS00050"
                     /pseudo=""
     CDS             <10595..11307
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037846392.1"
                     /locus_tag="N7E53_RS00050"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VGPRLAHQAVTWLLERYGSPSALRKAGRRRLVELIRPQALAWPSG
                     "
     gene            <11371..11538
                     /locus_tag="N7E53_RS00055"
                     /pseudo=""
     CDS             <11371..11538
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377440.1"
                     /locus_tag="N7E53_RS00055"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="TPTEKSHNQALAAARALVERGVARLES"
     misc_feature    11715..11735
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.8"
                     /tool="antismash"
     misc_feature    11740..11753
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 21.12"
                     /tool="antismash"
     gene            11784..12245
                     /locus_tag="N7E53_RS00060"
     CDS             11784..12245
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045303.1"
                     /locus_tag="N7E53_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CrcB family protein"
                     /protein_id="WP_058915284.1"
                     /transl_table=11
                     /translation="MSPKVALNALTHPDHHPGGQGPVLVAVAVGGAVGAAARYGAELLW
                     PTGPTDFPWTLLGINTVACFLMGVLMVTLKLRFPAAPRMVSPLLGTGMLGGFSSFSHYI
                     DDVRQLSDAARPGYAVANLLLTVVAALLGVTMGALLAHAVLGSGTRGGR"
     misc_feature    12174..12176
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            12242..12616
                     /locus_tag="N7E53_RS00065"
     CDS             12242..12616
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523061.1"
                     /locus_tag="N7E53_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CrcB family protein"
                     /protein_id="WP_058915285.1"
                     /transl_table=11
                     /translation="MIDWLLVLAGAAVGALVRYLIGVDAKYRFSTGFPWGTLAANATSC
                     LVLGFVAQSATDGGLGHQQYLLLATGFCGALSAWSTFSYELLTMTSARRTAVAGGYLLT
                     TLAAGLVGAFAGATVANTLF"
     gene            12949..14319
                     /locus_tag="N7E53_RS00070"
     CDS             12949..14319
                     /EC_number="4.1.1.15"
                     /GO_function="GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180: Decarboxylase, pyridoxal-dependent"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984645.1"
                     /locus_tag="N7E53_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamate decarboxylase"
                     /protein_id="WP_261674435.1"
                     /transl_table=11
                     /translation="MNKETVAVNPLFARPGEKAKAEKFRIPDDPMLPETAYQIVHDEIM
                     LDGNSRLNQATFVGTWMDSHASKLYTEAFDKNMIDKDEYPQTAEIEDRCWKMLADLWHA
                     PDPTGTIGTSTIGSSEACMLAGLTFKRRWQLARRAAGKPADKPNLVMSSAVQVVWEKFC
                     NYWDVEARYVPISEEHKTLDGHDLASYVDENTIGVVAILGVTYTGMYEPVAKVSDALDA
                     IQRDTGLDIPIHVDGASGAMVAPFLQPDLVWDFRLERVHSINTSGHKYGLVFPGLGWVV
                     WRSADLLPEDLIFRVSYLGGDMPTFALNFSRPGAQVLLQYYQFLRQGRTGYRAVQKASQ
                     DVARFLADEIAGMDAFELWNDGSDIPVFAWRQKAGHTENWSLYDLSDRLRMKGWQVPAY
                     PMPDNLNDLTVQRIVVRNGLSMDLASSLLNDIRTSVRFLDDLDGPMPSEGHNSGFHH"
     gene            14319..15788
                     /locus_tag="N7E53_RS00075"
     CDS             14319..15788
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1038: phenylalanine-specific permease"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012544.5"
                     /locus_tag="N7E53_RS00075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid permease"
                     /protein_id="WP_261674436.1"
                     /transl_table=11
                     /translation="MASSSQPAVPEPDAAAKSRTGRTYLSVFNLAILTVVVVASLRSLP
                     TMATYGLGSITLYIIPALLFLVPVSLVAAELATGWNGGVFVWVREAMGVRWGFTAIWLQ
                     WIQNVVWFPVQLAFIAASFTFVFVDEKLSGSGLYTAIVIVVLYWVSTALTLRGGNLFAK
                     VGSWGGLIGTIAPGAILIVLGACWLGTGQESQVPLKWGDVVPPFTGFASLVLIVSNFLA
                     YAGMEVNAVHANSMRNPGKGYPKAVFIALFGILGVFMLPTIAVAVAVPKGELGLTTGIN
                     LAFKVFFEHWGISWATAVVSAAISLGALASVVTWIAGPSKGVLAAAETGLMPLWLQKRN
                     KAGVQQGILMVQGLIVTALAALFIAIPNVSTAFFTLVDMAAALYLIMYMLMFVSALRLR
                     RTRPDVTRAYRVPAIKWVAGIGFAASAAAFVLGFVPPSGLSGIPPHLYPILMVVVIILL
                     GIPPLLFYRFRKPSWNRGQRADNHTPTATEG"
     gene            15790..16170
                     /locus_tag="N7E53_RS00080"
     CDS             15790..16170
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N7E53_RS00080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261674437.1"
                     /transl_table=11
                     /translation="MRGNATGAAASLIALTLAILTACSSTSSSAENDKAARFTAKVNEA
                     GVGPAMTPRIAKSLYGNDGGAICAALKDNPAGLLLGWGRVTLTATPEEHADDLVAYDRL
                     VVQVYCPEHLDTFEDLTDRINR"
ORIGIN
        1 ccccaccgaa gacaaggccg gccaccaaag tgagccgacg gccacgcaac cgcggcaacc
       61 cacaccacaa tcacaacgcg gacgggcctt gacccttgat cctggacaca cgagacactg
      121 gatcctgagg atctgagaac ggacatctcg tggtcatgaa gaactatccg ccgcagttca
      181 aggcggacgc ggtcgcgctg taccggtcgc ggcccgaagc cacgatcagg caggtcgctg
      241 ccgatctggg gatcaatccc gagaccctgc gtaactgggt ccgggccgct ggtgcgagcc
      301 ggccccgggg acgccgggcc gaggtgccgg ccgagccgcc cacgccgttg gaggcggaga
      361 acgccgctct gcgcaggaag gtccgcgagc tggaggagga acgcgagatc ctgcgcaagg
      421 cggcgaagta tttcgccggg gagacgcgct ggtgaaccgc ttccagttcg tcgccgacca
      481 ccaccgccgc tacggcgcga agcggctgtg caccatcctc ggcatcgccc gctccagctt
      541 ttactactgg cgccgcaccg ctgcggaccg ggccgcccgg caggcggccg acgctcgcct
      601 tgccgcccgg atccgggccg tgcaccgcga gtcggacggc acctacggcg tcccaaggat
      661 cactgccgag ctccgcgagg cgggcgagcg cgtcaaccac aagcggatcg cgcgcgtcat
      721 gcggggtgcc ggtctggcgg gcgtgcgtct gcgccgcaga caccgcacca ctgttgcgga
      781 ccaggccgca gcgaaggccc cggacctcat cggccgcgac ttcaccgcaa gcaagccgaa
      841 cacgaagtac gtcggcgaca ttacctatct cccgctggac ggcgggaggt tcctctatct
      901 ggccaccgtc atcgacctcg cctcacggcg ccttgccggc tgggcgatcg cggatcacat
      961 gcgcaccgat ctcgtcaccg acgccctggc cgccgccgaa caaacccgcg gcagcctcgc
     1021 cggggcggtc ctgcacaccg atcacggcgc ccagtacacc agccgggcct tcgccgacgc
     1081 ctgccgcaag gccggcgtcc gccagcccat gagcacgatc ggctccagcg cggacaacgc
     1141 gctcgccgag tccttcaacg cgacgttcaa acgcgagacg cttcagggtc gaaagcactg
     1201 gtccaccgag agcgaggccc gcctcgacgc cttccgctgg ctgcaccgct acaacacccg
     1261 acgccgtcac tcccgcctcg gacaccgcag tccgatcgcc tacgagaaca ccatccgaac
     1321 gacatcaact acgctggcgc aagccgcata acccgtgtcc aggaaccggg gtcaaggccc
     1381 atggaggccg cgcaacgctg gcccgacatt ctgagcctca gcttgcaccc cggtgtggcc
     1441 aggaccgaca tcaaccgcga tacatcactg cgcctcttct tccgttacgc gccgttcctg
     1501 atcagcccgg aaaagtcagc ccggcgcttg gtacgactcg cggtgacacc ggccgaggaa
     1561 ctgaccagcg gagccctcta cggcccccgt catgccatcc gtccgaaacc ggatattttc
     1621 aacgcggact acgcagcacg tctatgggcg acctccgatg ccgccctcgc cgaaccggcg
     1681 acgtagcgct gttcttcgac gagagaactg ctggccttcg aagtgtggtg tcggagggct
     1741 gacggcccgt gatccctgcg gtatgccgag tacatgaggt atgccaaggg cggaggcctt
     1801 gccgatgaac gccgcgtctt ccattcccga gttgcccgga agggctgcgg cccctgcgcg
     1861 acaagcacgc caccggcgac ggttgagcgc cgggagccgg caggccgcgg gactcagctc
     1921 tggacgcacc agagctgagt cgttgaccgg aacgcatgac ggccgtgccc cgaccggtgt
     1981 gagccagagc gcgacctcag cagcgcaggt gcaccgcttg tctgttgccc tatacaggct
     2041 gggccgtggg caggatggtc agttcactga tgttgacgtg cctgggaaga gcggcaatga
     2101 aggcaacggt ctctgcgatg tcggtgctac gcaggcactc gatgtcgtgg agcaggccgg
     2161 ccatcagggc gctggcgtcg gggtcggtga cgtggtcggg tagttcggtg tcgaccatgc
     2221 caggctcgat ggcgctcacc cgcacgttct tccggcccag ttcgactcgg atgagacggg
     2281 tgaggtggct gatgtacgcc ttggtccccg agtagatcga gaacttctcc agaatgcgtg
     2341 tcgccgcgat ggaggaggtg ttgatcaggt cggcggactt tccgcgctcg gcgccggcag
     2401 tcaggtgcgg gaggaaggca gcgatcacgt tcatcactcc cgtgacgttg agatcgatct
     2461 gcctctgcca gtcctcgacc ttgagttcgt cggtggcgga gatcaactgc acgcccgcgt
     2521 tgttgaacac caggtccgcc ggtcccagcg tgtccgccac ctggtcggcg gctgtgcgga
     2581 cggccgtccg atcggtgaca tcgacggaca ggggtagggc ctttccgccg gccgcggtga
     2641 tgtcggcggc gagggagtcg agcttttcct ggcggcgcgc gatgacggcg acagaggctc
     2701 ccagttcggc aagccggggg gcggtggccg cccccatgcc gctggaggct ccggtgacca
     2761 cggccacccg gctgtccaac ggccggccgg tgagcaggta cgtcatggtg agagacattt
     2821 cctgtgttcg gaatgggttc ggggtggtca ggcggcgtcg tcgcaggccg tggcggcgga
     2881 cttgtcctgc caggctgcga ggtcctcctg aaggctgcgg atcttgacca gctggcgttc
     2941 gatcgcatcg cgccccaggg ccaggtgtcc aggcagcttg tcctggcagg tcacctcgta
     3001 gatcagggcg gcgcccttga ccgggtcgcc gagctgggtg tggttggcgg tgtcgatcca
     3061 gtccagggtg gcgtgggccg gcgtgccgtc gtaggcgggg atacgctcgg ccgcggtcga
     3121 caaggagctg gcgtcgagga agtcggtgcg gaagaccccg ggctccacga ccatgctctg
     3181 aatcccgaac ggctccaact ccaccgcgag cgcctcactg ataccggcca ccgcgaactt
     3241 cgacgcgctg tacatgctca cccccggctc cccctcgaag ccggaacgtg aaccgatgtg
     3301 cacgatcttt ccggctccgg ccgccctcat cacgggcagg accgcgcggg tgacgttgat
     3361 cagcccgaag acgttgaggt cgaacaggga ccgcgcctcg gcgtcggtaa tctcctccag
     3421 cgcgccgagc agaccccgcc ccgcgttgtt gacgaggacg ttgatcgtgc cgaagtgctc
     3481 cacagccgtc tcgactgcgg cggtgatgtc ctcactgcga gtgacgtcga gggcgacacc
     3541 gagaacgcgg tcgtgccggt acagctcctt cggcagtttc tgcgggtcac gggcgccgac
     3601 cacgacgttg tcgccgccgt tcagtgccgc ccgcgcgatc tccagtccca gcccgcggga
     3661 gccgccggtg atgaaccacg tcgccatggg atgcctccat ctgttcgctt cgcccgacac
     3721 cccttgcggt gcgggctccg gcatccactg aaacagctct gagcaggaga aatcggcttc
     3781 aaaagggtcc gcctggtcgg gggacaaagt gggccaccct cccgccgccg aaacctcggt
     3841 gaacgcgcga ccctgggtat atggaccgtg agcactccac cagcgcatcc atggcggaat
     3901 tcctccgctc cgcccgcacc cgcctcactc cgcgggaagc cgggctcgac gacccgggtc
     3961 agggccgacg ccgggtgccg ggcctgcgac gggaggaact cgcccacctg gccggggtga
     4021 gcgtggacta ctacacccgc ctcgaacaag gtcgcagccg cagcgcctcc gcggaggttc
     4081 tcgacgcgct cgccaccgcc ctgcacctgg acgacgccga gcgcagccac ctgcacaccc
     4141 tcgcccggcc acggccccgg caggcccgtc ggcgcccccg cccccagcgt gtccaccccg
     4201 ccacctggga cttgctggag accctccagc aggccggccg ccccggtttc ctcctcggac
     4261 ggcgcctcga cgtgctggcc cataacccac tcgccggacg tctgatcacc gacttccgcg
     4321 ccctccccgc ggccgagcgg aaccaggccc gcttcgtctt cctcgacccg cacgcacgcg
     4381 agctgtaccg ggactggccc cgcgtcgccg ccgacaccgc cgccatgctc cgcctcgacg
     4441 cgggccgcca ccccgacgac ccggccctca gtgcactggt cggtgacctg tccatacaca
     4501 gcgaggaatt ccgccgcttc tggtccgaca acaaagtcca ccaacgcacg accggcacca
     4561 aggactacca ccacccgctc gtcggcgacc tcaccgtcac ctaccaggcc ctcacccccg
     4621 cagacgaccc cgaccagatc ctcttcgtct acggcaccga acccggcagc ccgtccgaga
     4681 cctcgatgcg cgtgctggcc caatggagca ggacggcagc cgataccggc agcctcacaa
     4741 cgcaaacgcc aggagcgcac gactgacgcg agcccgctgc ggtcgtcacc aagtacggga
     4801 gcacccgccg cacagcagcc tctcccccac gccccgggtg cttcgcactc cgggacagta
     4861 aacgatcaac ggctggtggt gcccggggca agcaggccga tcagctcgag atcacctccg
     4921 aggagggtgt gaagtaacgg cgcacgttct ggggcttctt gtgccgggac ttcgccatca
     4981 gcatcagcag agaggcaccc tgctcctcga ggcgggtcgg ggcgcaatgg cgatactggt
     5041 gcagctccca gccggccccc ccggccacga ggtatgctcg tcgagcaggg tccgaccgca
     5101 ggaaaggcgg gcaaggccgg tgcccgggca cacgtcgcgt gcgctgaggg ccatccccgg
     5161 gccgggcccg gtgggtgaca aacacgggcc cgcgggtgcg gtcgcgccca gccgggacag
     5221 caggcgggcg gtgccgacat cccattagag gaacccggca cgaaagtcct tgcgcgcctg
     5281 accacggcgg cgggcctggc tccgggcgtc aggtcctcga ccttcacgtc ggaggtctcc
     5341 tcccaccgcc atgcggtctt gtagagcatc cgccacggag ctttcccgcg caggtggacc
     5401 tcgcggcggg caatggatcg gtcccggccc acttggagcg ggccgggagt taggaagtcc
     5461 ggtaccgaca cccgcttcgt ccggaccagg accgccggct tgtcctagcc gtactccgcg
     5521 caccagctca gccaggagag caccgccccc ggcgggcgtc cgaggtgttg ccagtcccgg
     5581 cgtcccacag gagtttcaaa gcctcaccga taggcaccgt cacgaagttg acgtcgttgc
     5641 gcgtggacgc gagccacagg gtcgaccggg tgcacccgga cgcactgggc aggggcggtc
     5701 tgccggtgcg gagtgcaccc gagcgccggg gtttcctgcc gcaggcgggc cccaccacgt
     5761 tccgcagccc gccgcccaag gccggcgtgc tcttcggcgg cgacatgtgc agatcacgac
     5821 ccgacggatg cgtgtcagtt ggcttcaccc ctgtctgccg gcacacgccg tcgacggagg
     5881 ccagtcgacc acgactgagt gcggtcggtc tgcgcgtgga gagcgggcgc ttgacaacct
     5941 cgtatgcgag tggttaatta cttacatagt tacggcgagc aggagcgtca gagatacgag
     6001 catctgccaa agctccagtc agcctggccg tatgtaacct ggacgaggtc gaatcaagcg
     6061 ggttgatcac tcaagagggc atccttagcc tttcatgagg accaactatg cgatcgaccg
     6121 ctttaaagat ggggcaagac gtgactgaga cggtttccac ctcgcagaac accgacgaca
     6181 tccgggccgg tgcgcagcag gcgttcaaga accacctgga gtacctgtcg tcggggcgga
     6241 tcccggagtg ggtggatctg ttcaccgagg acggggtgct ggagttcccc tacgcacccg
     6301 agggctatcc gaccaagacg gtgggcaagg aggggctgtt cgagtacatg aagaacttcc
     6361 cccagcactt cagggtggag ttcatcgacc tgcacttcca tgagacggtt gatccgtcgt
     6421 tggtgatcgc ggagttcaag agccggggta ccgcgctggc cacgggccgg ccctacaatc
     6481 agacgtacat ctcggtggtg gagacgaagg gcggcaagat cagccgttac gtcgacttct
     6541 ggaacccgct ggtgggcatg caggccctcg gcgacgatcc ggctgacatg gtcgccgcct
     6601 tcagcaacga ctgaggtcgc acgtcactct ccggcagtgg gcagggtcgc ccacctcgac
     6661 ccggccgtgg cgacccaccc acccggaggc gatcggggcg acttgacgct ccgagcgtgg
     6721 aaagtgtttg gatactcata tggagacaag gacgcggcag cgttccactg cggaggagcg
     6781 ccgtgccacg gtcatgcgta cggcgatcaa gacgttcgca gagcgtggat actacgggac
     6841 ctcgacgatg gacgtggcca aggccgccgg catctcgcag ggttacctgt accggctgtt
     6901 caagggcaag gagacgctgt tcgcggccct ggtcgaccac tgctccgccc gcctgctgga
     6961 agacgccgcc gcggcggccg catctgtgca gagcgcccgc ccggaagagg tcctcaaggc
     7021 gctgaccgcc tcctacaacg agactatcgc cgaccgggac gtgctcatga tcctcatgca
     7081 cgcccagtgc gccgcgagcg agccggtcat cggtgaagcc gtgcgcacct gctacgccaa
     7141 gcaggtcgag tatgtccgcg ccgtctccgg cgcctccaac gaacagctcc gccgttactt
     7201 cgccgacggc ctgctcagca acgtcatgat ggcgatcgac gccacgactc tcgacacccc
     7261 ctgggcgcgc gcactgcgcg cctagccgaa gcattccgcc gggccgcccc gtgcggccct
     7321 ctttttcgcc ctaacaagta agcggtcaac cgcttacgta ctgcctgaga ggcttacccc
     7381 ctcatgccaa cccactccaa gctccgctcg accgggcttc tgctcgcctt gctcgccttc
     7441 gcccagttca tcagcgccat cgactacaac atcgtctacg tcgcactccc ggagatcggc
     7501 cgcgaggtag gcttcgacgc gcagaacctg cagtgggtcg tcagcggcta cgccgtcgcc
     7561 ttcggcggct tcctgctcct gggcggccga gccgccgacc ttctcggcca ccgtcgtatg
     7621 ttcaccaccg ccctggtcct ttatggcctg tcttccctgg ccggcggact ggccaccgac
     7681 cccgggctgc tggtggccgc acgcgcggtc cagggcctgg gcggcgcgct cctgctgccg
     7741 gccacgctct ccctgatcgg caccaccttc cccgagggcg ccgagcgcaa ccgggcgctc
     7801 gcgatctggg gcggtgccgg agccgtcggt ctggcgctcg gttccctgct gggcggcgtg
     7861 ctgaccaact acctcggctg ggagtccgtc ttctatgtca atgtccccct cgccctgggc
     7921 gccgctgccg cggctttccc gatcatcacc aaggacgggc cgcgtgagcg gggccgcagc
     7981 ttcgacttga ccggtgcgct gaccgccacg gtcggcttct ccgccctcgt cttcggcgtc
     8041 gtgcagggtc ccgaggccgg ctggggttcc gtcgaggccc tcattcccct cctcggcggc
     8101 gcactgttca ccggactgtt cctgatcgtc gagtcccgtt ccgctcaccc gctgatgccg
     8161 ctgcgcctgt tccgcaaccg cagtctgggc gccgcgatgg gcgtcacctt cgtcttcatg
     8221 ggcaccttcg gcgcccagta ctacttcttc accgtctacc tgcagaacgt gggcggctat
     8281 ggcaccctgg ccaccggtct ggcgttcctg ccctcggcgc tggtggggct ggtcggcagc
     8341 aagctgagcg agaagctgct cggcaaggtc ggcccccgta cgacgatcgt caccggtctg
     8401 ctcgtgggcg ccgcgggcat ggccgtcctg gcgctgggca tgtcgccgaa cggcggctat
     8461 cccgccttgc tgcccggcgt gatcctgctc agcctcggcc agggcatcgc ctgggctgcg
     8521 atgttcgccg cggccaccag cggcatcgac gcccgccacc agggcatcgc ctccgccatg
     8581 gcctccacca cgcagcagat cggctccgcc gtcggtctgg cgatcctggt cgccatcgcc
     8641 aacagcggcg tcgaggtcac caccggccca gacctcgtcc ccggcctgcg caccgcaggg
     8701 ttcaccgccg ccgccctcac cctcctcgga gtggcgatcg cccttaccct gcgtcagccc
     8761 ggcaccaccc ctccggcccc caccgcgacg cagaccacgc ataggccgga agcaagcatc
     8821 tccgcttgcc cccctgagcg cagagcacac agaccctctc gccggggcgg ccaacccgcc
     8881 cggcgagagg gtcatcttgc gtcgtgcacc cttgacgctc cccactccca cccgcaggag
     8941 caggacggtc aaggcgcctt cgacccgtgc gaccaccgac gagctgacca aggccccctt
     9001 ctcggtactg gaccgctccc gcacccgcac gggcaaagga gcgggacgca ggcactgagg
     9061 gccaccattc gcctcgccca gcgggaaagg cctgaggatc gtcgtccccg acacggacac
     9121 gaggatagga ctcgatggcg tgcttcacct gggaagtgcc tccgaaggtg gcagtttcga
     9181 ggcggacggg gcgacaagcc gcgcgagccg tgtccgtgca gccctcggac tgttgagccc
     9241 cgcacgacac ccattcccaa gccgaggctc tcctgaccgt catctgcaat tccagccgaa
     9301 cctccgtcgg ctcaggtgtc gttgagatcg gacccgtcgg tcccgctcgt atgcgcgccg
     9361 ctttcaccgc ttgttccgct ctcgaggatg gagacgatcg agaacagcga gaagcagatg
     9421 cctcccagac ccaccaggac gcgggccgcg acgaccacgg cgggatcggc ggactcgaag
     9481 aggaacgccg agaggatcag acagaggagc gcagtgccga caggaatcag cggaacgcgg
     9541 ttggccagga gagcctcgcg ccgccagacg agtgcgagca gcagcacctt gctgaggatg
     9601 ctccagcaga tcagtcccag tccgatcatg acgtatgccg caggcagttg cgagggccgg
     9661 gtgtgcacgg agaggacgat cagcgcccag acgatgccca ccgcgcctgc gacggccgcc
     9721 gacaccggcc atagaacacg atcccggtgg gtgtaggtgt tctggatctg tcgcagaatg
     9781 ctggccacga gcccgatcag acacgtgcag acgagtgcca gccccaccag cacgtgcccg
     9841 gcggtgaaac gccccgcagc cagccctccg cctccgcccg ccagcagagt gaaggcccac
     9901 gcccaggtga tcagggaggc caggaccggg agcgtcagca gagtacgggt ggcgagcgga
     9961 ccgaagggag ccggtgacag aggcggagat tcgtcgggca gtgcagagtt cctggtgatc
    10021 tgcacgaacc gggtggaggc cgtggcgacg cacgagacac agacacagat catgccgatt
    10081 ccgaagacta cgtgaccggc gatgaaatcg gggggtggcc gaggcgtctc ggtcagaaga
    10141 gaaagcccgt agcctatagc gacaccgctg atcgcgaatc cgaataccgg atacacgatc
    10201 cgatccgtgg gcgtgtagcg gtgaatgagc tgcctgatga tcgtggccgc ggtgctgaac
    10261 aggcacaggc agatcgctga caggaagatc agcaccagcc cagcgacgcg cgcgctggct
    10321 ccggacgcat gagcaaggac gtagccgccc agcgcggcgg acaccgcgcc catgaggacg
    10381 gggatggcgc ggaacaggac gctgattccg tagttcatgg cgagggctcc tcaggacgac
    10441 atctgaggcc atcgtgatgc ggaggtcgtc gtgctgccag cggggaacac agacggggat
    10501 cgctgtcgag ccgcagcccg ggaacgtgcg tgcagcgtgc cccacgggcc cctgtcgttc
    10561 gagcgtctcg gctccggata gacggatcac gcgcgtcggc ccccgcctcg cccaccaagc
    10621 cgtgacctgg ctgctggagc gatacggctc cccgtccgcc ctacgcaagg ccggccgccg
    10681 cagactcgtg gagctgatcc gcccgcaagc tctcgcatgg cccagcggct gatcgacgac
    10741 atcttcgacg cactcgacga acaggccgtc gtcatccccg gcaccggcac cggcaccggc
    10801 accctcggca tcccctccct gcccgcgtcc tggccgccgt ccacacgcag cgccgggtga
    10861 cggaagccca gatcaacgcc ctgctgcagg ctcaccctct tttcccggtc ctgacgtcga
    10921 tgcccggcgt cggcgtcagg accgccgccg tcctgctggt caccgtcggc gacggcaccg
    10981 gctttcccac ctccgcccac ctcgcctccc tacgcggaac tcgccccggc aacgaaatcg
    11041 tccggcacct cgatccacgg tgagcacgca cccaccccga ggcggcaacc ggcagctcga
    11101 cgggcgctgt tcctgtccgc cttcgcgtgc atgaacgccg acccggtctc ccgcacctac
    11161 tacgactgcc ggcgagcacg cggcaagacc cacacccaag ccctcctccg actcgcccgc
    11221 caacgcatca gcgtcctgtt cgccatgttc cgcgacggca ccttccgcga atcccggaca
    11281 ccgaaagacg ccgacgtcgc tgcataacct caacaacccc gaaccccacc ccgaacccga
    11341 caggggtgcc ttgacggaag acatcgaggc acccccaccg aaaagtccca caaccaggca
    11401 ctggccgcag cgcgagcgct cgtcgaacgc ggcgtcgcga ggctggagtc ctgacgcata
    11461 ttccgcaggt cccgatgcag cctcaaccgc atgacgtcaa tcgccaaggc catcctcacg
    11521 ctggagcggc aacgctgaag aagttcactg ccgggtatcg aggatgagcg ccccgcgcga
    11581 cggcgcgggc acagtacgtg cagacccgtc accgacggca cctcatcgac ggcggcggct
    11641 gcctcatgcc tcgctcgttg ccctgtacgg ccaccatccg atcggcgggg gtaactcgat
    11701 gaagtccaga tccgttcggg tcgaacggac gaccacgcgg ccacgctccg tgaactacca
    11761 agtaccttct ttatagtgcg tccatgtccc cgaaggtggc tctgaacgcc cttacccatc
    11821 cggaccatca tcccggtggc cagggccccg tcctggtagc ggtcgctgtt ggcggggcag
    11881 tcggcgccgc ggcccgttac ggcgcggagt tgctgtggcc cacgggtccg acggacttcc
    11941 cctggacact cctcggtatc aacacggtgg cctgcttcct gatgggtgtc ctcatggtca
    12001 ccctcaagct gcgcttccct gccgcccccc ggatggtgag cccgctgctc ggcaccggga
    12061 tgctgggcgg cttctcctcg ttctcgcact acatcgacga cgtacgtcag ctgtccgacg
    12121 ccgcccggcc cggttacgcg gtggcgaatc tactgctgac cgtcgtggcg gcattactcg
    12181 gagttacgat gggggcgctc ttggctcacg cagtcctcgg tagcggaaca cgaggagggc
    12241 ggtgatcgac tggctgctcg tgctcgcggg cgccgccgtc ggggccctcg tgcgctatct
    12301 gatcggggtc gacgccaaat accggttcag cactggtttc ccctggggga cgctggcggc
    12361 caacgccacc tcctgccttg tgctgggttt cgtcgcgcag agtgccacgg acggtggcct
    12421 cgggcaccag cagtacctgt tgctggccac cggcttctgc ggcgcgctgt cggcttggtc
    12481 taccttctcc tacgaactcc tgacgatgac ctcggcccgc aggacggcgg tcgccggcgg
    12541 atacctgctg accacactcg cggcagggct cgtgggggcc ttcgccgggg ccacggtcgc
    12601 gaacacgctc ttctgacagg ccgggtccgg agggcgtcca cggccgcggc ggtgtccctc
    12661 gtcgggtcgc agggccggcg ccccggaggg cggcgagtcg gaatcggggg ccgccgtacg
    12721 gcaaagccct ccgtgcgtac agcgagatgc cgttcgcgcg tatacgtggg tcagaaagcg
    12781 gcgcgaaacg cgccacgact tcgggcggca gtcgccacgt actgcatttg gacgaaacat
    12841 gacagccgaa cggctcagat tttcgtggct actgacggct aaaaggggaa ctcgtggctg
    12901 ctgacgggta aaaagggatg acgcacgtga catgagaggc ttgccgtgat gaacaaagaa
    12961 actgtggcgg taaaccccct gttcgcccgc ccaggggaaa aggcgaaggc cgaaaagttc
    13021 cgcatcccgg acgatcccat gcttccggag acggcgtatc agatcgtgca cgacgagatc
    13081 atgctcgacg gaaactcccg tttgaaccag gcaactttcg tgggaacctg gatggattcg
    13141 catgcttcga agctgtacac cgaggcattc gacaagaaca tgatcgacaa ggatgagtat
    13201 ccccagacgg cggagattga ggaccgctgc tggaagatgc tcgccgatct gtggcacgcg
    13261 ccggatccaa ccggcacgat cggcacttcc accatcggat cgtccgaggc gtgcatgctc
    13321 gcgggcttga ccttcaagcg tcgctggcag cttgcccgcc gcgcggcggg caagccggcc
    13381 gacaagccga acctggtgat gtcctccgca gtgcaggtgg tctgggagaa gttctgcaac
    13441 tactgggacg tagaggcgcg gtatgtgccc atcagcgagg agcacaagac tctcgacggg
    13501 cacgacctgg cgtcctacgt ggatgagaac accatcggcg tggtggcaat cctcggggtg
    13561 acctacaccg gcatgtacga accggtcgcc aaggtctccg acgcgctgga cgcgatccag
    13621 cgcgacaccg gactggacat ccccatccac gtcgacggcg cgtctggggc gatggtcgcg
    13681 ccgttcctgc agcccgatct ggtgtgggac ttccggctgg agcgcgtcca ctcgatcaac
    13741 acgtccggcc acaagtacgg gcttgtcttc ccgggtctgg gctgggtggt gtggcgcagc
    13801 gccgacctgc tccccgaaga cctcatcttc cgcgtcagtt acctgggcgg cgacatgccg
    13861 accttcgcgc tgaacttctc ccgcccggga gcgcaggtgc tgttgcagta ctaccagttc
    13921 ctgcgccagg gccggaccgg ctaccgggcc gtgcagaagg ccagtcagga cgtcgcacgg
    13981 ttcctcgccg acgagatcgc cggcatggac gccttcgagc tgtggaacga tggctcggac
    14041 atcccggtgt tcgcctggcg gcagaaggcc ggccacaccg agaactggtc gctgtacgac
    14101 ctgtcggacc ggctccgtat gaagggctgg caggttcccg cctatccgat gccggacaac
    14161 ctgaacgatc tgaccgtgca gcgcatcgtg gtacgcaacg ggctgagcat ggacttggcg
    14221 agctcgttgc tgaacgatat ccgcacctca gtgcggttcc tcgacgacct cgacgggccg
    14281 atgccctccg agggtcacaa ctccgggttc caccactgat ggcctcctcc tcgcaaccgg
    14341 cggtgccgga accggatgcg gccgcgaagt cccggacggg ccgcacgtac ctgagcgttt
    14401 tcaacctggc catcctcacc gtcgtggtgg tcgccagcct gcgcagcctg ccgacgatgg
    14461 ccacctacgg gctgggcagc atcacgctgt acatcatccc ggccctgctc ttcctggtcc
    14521 ctgtgtccct ggtggcagcc gagctggcga ccggctggaa cggtggcgtg ttcgtctggg
    14581 tccgtgaagc catgggcgtg cgctggggat tcaccgcgat ctggctgcag tggatccaga
    14641 atgtcgtgtg gttccctgtc cagctcgcct tcatcgcggc cagcttcacc ttcgtcttcg
    14701 tcgacgagaa gctctccggg tccggcctgt acaccgcaat cgtcatcgtc gttctgtact
    14761 gggtgtccac cgccctgacg ctgcgtgggg gcaacctctt cgcgaaggtc gggtcgtggg
    14821 gtggcctcat cggcaccatc gcaccgggtg ccatcctcat cgtgctagga gcctgctggc
    14881 tcggcaccgg ccaggaaagc caggtccccc tcaagtgggg cgacgtggtg ccgccgttca
    14941 cgggcttcgc gagcctggtg ctgatcgtca gcaacttcct ggcctatgcg ggtatggagg
    15001 tcaacgccgt ccacgccaac agcatgcgca atcccggcaa gggctacccg aaagcggtct
    15061 tcatcgctct cttcggcatc ctgggtgtgt tcatgctgcc cacgatcgcc gtcgcggtcg
    15121 cggttcccaa gggcgagctc ggcttgacca ccggcatcaa cctggccttc aaggtcttct
    15181 tcgaacactg gggcatctcc tgggccactg cggtggtctc cgccgccatc tcccttggcg
    15241 ccctcgcctc cgtggtcacc tggatcgccg gccccagcaa gggtgtactt gcagcagcgg
    15301 agacagggct catgcccctg tggctgcaga agcgcaacaa ggccggagtc cagcagggca
    15361 ttctcatggt ccaggggctg atcgtcacgg ctctggcagc cctgttcatc gccattccga
    15421 acgtgtcgac ggcgttcttc acactcgtcg acatggccgc agccctctac ctcatcatgt
    15481 acatgctgat gttcgtctcg gcgctgagac tgcgccgaac ccgcccggac gtcacacgcg
    15541 cgtacagggt gccggcgatc aagtgggtcg cgggtatcgg gttcgcggcc tcggcagccg
    15601 cattcgtgct cgggttcgtc cccccgtcgg ggctgtccgg aattcccccg catctctacc
    15661 ccattctgat ggtcgtcgtg atcatcttgc tcgggattcc gccgctgctc ttctaccgct
    15721 tccgaaaacc cagctggaac cgtggacagc gcgcggacaa ccacactccc actgcgacgg
    15781 agggctgaca tgcgcggaaa tgcgaccggt gcggccgcgt cactcatcgc cctcaccctc
    15841 gccatcctca cggcgtgctc cagcacgagt tccagcgccg agaacgacaa agcggcccgg
    15901 ttcacagcga aggtgaacga ggccggcgtc ggtcctgcga tgaccccccg catcgccaaa
    15961 tcgctctacg gcaacgacgg aggcgccatc tgcgctgcac tcaaggacaa tccggccggc
    16021 ctgcttctgg gatgggggcg cgtcaccctc accgcgacac cggaagagca cgccgacgac
    16081 ctggtcgcct acgaccgctt ggtcgtccag gtctactgcc cggagcatct cgacaccttc
    16141 gaggatctca ccgaccgcat caaccgatga acaaccgccg cacggtccgc caccccgccc
    16201 cggggtcggg tgtcacgacg ctttggtcct ggtcgaggaa tggacgaacg tcgtcgatga
    16261 cgttgcggat cgtgccgcgg ctgacgccga acaggtcggc ggggtctgct gaccgcacag
    16321 accgcgatgc aagtggacgg ttgccaggat tcggtccaag tcggtgatct tctggggaag
    16381 agaccgcccc gggctccggg gcatcggtca gggcacggca gtccggtcga ccagtgagct
    16441 ttccatctcc cccaatgtgg agcggaccga cctgcgtcgg ccgggaaggt agcgggcagg
    16501 catccgccgg ggcgatgtcc ccgaaaagca cggctgccat gcggcgggat ggaccacagg
    16561 gtgatggccc atcacgccaa gccaagccgt gtcagcgccg ggtcagctcc gcga
//
