LOCUS       NZ_CP104794            22707 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8471484
            Orig. end                         :: 8494191
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22707
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="34"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22707
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22707
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12707](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12707)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    72..86
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            1217..2638
                     /locus_tag="N6J21_RS37315"
     CDS             1217..2638
                     /EC_number="1.2.1.19"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145174.1"
                     /locus_tag="N6J21_RS37315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="WP_261720486.1"
                     /sec_met_domain="Aldedh (E-value: 1.5e-155, bitscore:
                     517.0, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADSDRLNHIGGKDVPAASGATLLLIDPATGLPHATAPLSGPEDV
                     DAACRAAEDAFENWASTTPGERQTALLRIADSLERTADRIADAEVADTGKPRSLFLADE
                     LPAIVDVLRYYAGAARNLPGSAAAEYTPGRTSVLRREPIGVCAQITPWNYPLMMAVWKI
                     APALAAGNTVVLKPSDTTPSSAVLLARLAAEHLPPGVLNVVCGDRDTGRALVAHPDVRL
                     VAVTGSVRAGQEIAAAAAADLKRLHLELGGNAPVLIHADADLDDAVEQLASLSFYNAGQ
                     DCTAATRILVDRRIHDTFLTRFTQRVARLRPGAPDDGHADFGPLANAAQLAAVRGLLER
                     LPAHGEIVSGGAVVDRPGYFHEPTVVAGVRQLDEIVQREVFGPVVTVQPFTDEAEAVRL
                     ANGVPQGLAASVWTRDHDRAMRATRALRTGIVWVNTHGTTVSEMPHGGVGHSGCGSDLS
                     LTGLLDYTQVKHVML"
     gene            2709..3551
                     /locus_tag="N6J21_RS37320"
     CDS             2709..3551
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043145.1"
                     /locus_tag="N6J21_RS37320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_261720487.1"
                     /transl_table=11
                     /translation="MTQTQNILVTGATGTVGRQVVAELLTRGHRVRALTRNAAKADLPA
                     GVEVVEGDLTDPDTLTPALEGVTGLHLITFGGAYFAPLQTGPRILELARAAGVRRVTVL
                     HGGEGTPLEDAVRADDGVDWTVLMPVEFMANALEWADGIVTRDEVREPFVSRLSAMVHE
                     GDIGAVAAVALTEEGHGGQEYVITGPEVLTVADKVAAVAGARGRAVSLVELTEEQAVEQ
                     WRAAGHTDEVIGFLLEAFGNTPEIGRTVTDTVEKVTGRPARTFRQWAQEHAEAFGPHA"
     misc_feature    2722..2736
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.78"
                     /tool="antismash"
     gene            complement(3616..4170)
                     /locus_tag="N6J21_RS37325"
     CDS             complement(3616..4170)
                     /EC_number="2.7.4.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA]"
                     /GO_process="GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526957.1"
                     /locus_tag="N6J21_RS37325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="adenylate kinase"
                     /protein_id="WP_261720488.1"
                     /transl_table=11
                     /translation="MRIILIGPPGAGKGTQAVALSERLGVPHISTGDLFRDHVARRTPL
                     GQEAQRYLDTGELVPDHVTAGMVRERLALPDARDGYLLDGFPRTVPQAQELAAMLTATG
                     TALDAVLELSVPEEVVVARLLGRGRADDTEEIIRNRQRVYEKQTAPLLAYYADLLVTVP
                     ADGPVPEITARALEGLSAPRV"
     misc_feature    4153..4168
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    complement(4153..4171)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.0"
                     /tool="antismash"
     misc_feature    complement(4156..4171)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     gene            complement(4289..4942)
                     /locus_tag="N6J21_RS37330"
     CDS             complement(4289..4942)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671052.1"
                     /locus_tag="N6J21_RS37330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_093720021.1"
                     /transl_table=11
                     /translation="MGHISTLTGRVVLVTGEGEPAPAVTASLVARGARVAVAGAPVPRG
                     SSVLALPFTPSDAAGPGRVVDTVVERLGRLDHVVNLVAGRPRTGPLMELDPLDLRETFQ
                     RDLVMPLSFVQRAYRRWMAAHGGSVVNVVAATGRGGARDATLAGLTELTESLAAELAPR
                     VDVHTLIPSPSLDAAAHLVGLAEVLCDLLTHRMDPAQGPVLVLSAEPDCASRAA"
     gene            complement(4961..6865)
                     /gene="acs"
                     /locus_tag="N6J21_RS37335"
     CDS             complement(4961..6865)
                     /EC_number="6.2.1.1"
                     /GO_function="GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016208 - AMP binding [Evidence IEA]"
                     /GO_process="GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (67-506). E-value: 2.5e-99.
                     Score: 324.6. Matches aSDomain:
                     nrpspksdomains_N6J21_RS37335_AMP-binding.1"
                     /codon_start=1
                     /gene="acs"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554932.1"
                     /locus_tag="N6J21_RS37335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetate--CoA ligase"
                     /protein_id="WP_093720020.1"
                     /sec_met_domain="AMP-binding (E-value: 2.5e-110, bitscore:
                     367.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPRSDHHSRHGQTAQPVDDRLAFWARQAGELDWDTEWSEVLDWS
                     DAPFARWFTGGKLNVAYNCVDRHVAAGHGDQVAFHWEGEPGDTRTITYAELRREVCRTA
                     NALLSLGLRAGDRVAIQLPMIPEAVFAMLACARLGLPHSVVFGGFSPAALQSRIEDAGA
                     RLLITSDGQYRRGRVEPMKANTDEATRDTPGVEHVLVVRRTGTDVPWTEGRDLWWHELV
                     DVQSDRHEPEAFDAEHPLFILYTSGTTGRPKGILHTSGGYLTQAACTHRTVFGLDPDTD
                     VYWCTADIGWITGHSYIVYGPLANRTTSVLYEGTPNTPHEGRHFEIIEKYGVSVYYTAP
                     TLIRTFMKWGEDIPARHDLSSIRVLGSVGEPINPEAWRWYREHIGAGRAPVVDTWWQTE
                     TGAVMIAPSPDSPSFKPGSAQTPVPGISAKVVDDQGVEVAKGESGYLVLDQPWPSMLRG
                     VWGDDDRYRQTYWARFAAQRYYFAGDGARYDDDGDIWLLGRVDDVMNVSGHRISTSEVE
                     SALVSHPAVAEAAVVGASDATTGQAIVAFVILREGAGGEVAVDALREHVAREIGPIAKP
                     RRILVVPELPKTRSGKIMRRLLRDVAENRQVGDVSTLADASVMDRIHAGLADASEQSG"
     CDS_motif       complement(5315..5377)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N6J21_RS37335_0002"
                     /evalue="5.00E-05"
                     /label="NRPS-A_a8"
                     /locus_tag="N6J21_RS37335"
                     /protein_end="517"
                     /protein_start="496"
                     /score="16.0"
                     /tool="antismash"
                     /translation="LGRVDDVMNVSGHRISTSEVE"
     aSDomain        complement(5348..6664)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS37335_AMP-binding.1"
                     /evalue="2.50E-99"
                     /label="N6J21_RS37335_AMP-binding.1"
                     /locus_tag="N6J21_RS37335"
                     /protein_end="506"
                     /protein_start="67"
                     /score="324.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="HVAAGHGDQVAFHWEGEPGDTRTITYAELRREVCRTANALLSLGL
                     RAGDRVAIQLPMIPEAVFAMLACARLGLPHSVVFGGFSPAALQSRIEDAGARLLITSDG
                     QYRRGRVEPMKANTDEATRDTPGVEHVLVVRRTGTDVPWTEGRDLWWHELVDVQSDRHE
                     PEAFDAEHPLFILYTSGTTGRPKGILHTSGGYLTQAACTHRTVFGLDPDTDVYWCTADI
                     GWITGHSYIVYGPLANRTTSVLYEGTPNTPHEGRHFEIIEKYGVSVYYTAPTLIRTFMK
                     WGEDIPARHDLSSIRVLGSVGEPINPEAWRWYREHIGAGRAPVVDTWWQTETGAVMIAP
                     SPDSPSFKPGSAQTPVPGISAKVVDDQGVEVAKGESGYLVLDQPWPSMLRGVWGDDDRY
                     RQTYWARFAAQRYYFAGDGARYDDDGDIWLLGRVDDVMNV"
     CDS_motif       complement(6092..6145)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N6J21_RS37335_0001"
                     /evalue="5.80E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="N6J21_RS37335"
                     /protein_end="258"
                     /protein_start="240"
                     /score="22.0"
                     /tool="antismash"
                     /translation="FILYTSGTTGRPKGILHT"
     gene            complement(6993..8474)
                     /gene="mftF"
                     /locus_tag="N6J21_RS37340"
     CDS             complement(6993..8474)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671054.1"
                     /locus_tag="N6J21_RS37340"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_261720489.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 4.8e-23,
                     bitscore: 80.6, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLAPAPPDLLAPPAGDRLPDGFTVELAPGSHRSADGRLMMGGSP
                     LRLMRLTEAAVRRLGDGRFSVTDGPSAALARRLLDAGIAHPRPAPVPVRDTTVVIPVRD
                     RADQLDRLLDALRRDPATTGLPVLVVDDASHSPAAVADVARRHGARLITHPYNQGPAAA
                     RNTGLRHCRTAFVAFCDSDVVPEPGWLAPLLAQFTDPAVALVAPRVVALPVPSPGLLDR
                     YEKLRSPLDMGAAEGPVVPVSALSYVPSATIVVRRAAVGAGFDTALRVGEDVDLCMRLH
                     EAGWRVRYVPSARVGHRHRTDLRNWLAQRASYGTGAAALALRHPGQVPPLYAAPWSLTA
                     CALLLRGRPVPVAAAAVLTGVTAVRVARRMPDADHPARAGALLSVAAVRGTAEQLLRCA
                     TRHHWPVALAAAAVDKRARWLLLTAAVADGLLDHRRSGSPLPPLTHLALRRLDDLAYGW
                     GVWQGAVRHRTTAPLRPRLALRMVSRWTGARPQPG"
     gene            complement(8471..9256)
                     /locus_tag="N6J21_RS37345"
     CDS             complement(8471..9256)
                     /NRPS_PKS="Domain: PKS_KR (5-177). E-value: 9e-11. Score:
                     33.9. Matches aSDomain:
                     nrpspksdomains_N6J21_RS37345_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671055.1"
                     /locus_tag="N6J21_RS37345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_261720490.1"
                     /transl_table=11
                     /translation="MTGPRVAVVTGAARGIGAATVRRLAGSGWAVVAVDRCADDPEVPY
                     PLAEPGQLDALAEEFELVRAVRADVRDEQALSTAVDLAEREFGGLDAAVAGAAVILGGA
                     PVWEQTDAQWRTLLDVDVLGVAHLARTAVPALLRRPEPRSGRFVALASAAAHRGLWRLG
                     AYCAAKHAVVGLVRGLACDLRGTGVNALAVSPGSTRTAMLRATAGLYGLTDVDTLAGRQ
                     LTERPLEPEEVAAVVAWACSADSVSVTGSVLHADGGFTS"
     aSDomain        complement(8726..9241)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS37345_PKS_KR.1"
                     /evalue="9.00E-11"
                     /label="N6J21_RS37345_PKS_KR.1"
                     /locus_tag="N6J21_RS37345"
                     /protein_end="177"
                     /protein_start="5"
                     /score="33.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAARGIGAATVRRLAGSGWAVVAVDRCADDPEVPYPLAEP
                     GQLDALAEEFELVRAVRADVRDEQALSTAVDLAEREFGGLDAAVAGAAVILGGAPVWEQ
                     TDAQWRTLLDVDVLGVAHLARTAVPALLRRPEPRSGRFVALASAAAHRGLWRLGAYCAA
                     KHAVVGLVR"
     CDS_motif       complement(9173..9235)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N6J21_RS37345_0001"
                     /evalue="3.30E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="N6J21_RS37345"
                     /protein_end="28"
                     /protein_start="7"
                     /score="10.1"
                     /tool="antismash"
                     /translation="VVTGAARGIGAATVRRLAGSG"
     gene            complement(9253..9999)
                     /gene="mftE"
                     /locus_tag="N6J21_RS37350"
     CDS             complement(9253..9999)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671056.1"
                     /locus_tag="N6J21_RS37350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_093720017.1"
                     /transl_table=11
                     /translation="MTPGPAPAPRLADLAWPDLTGRAPLVLLPLGSCEQHGPHLPLDTD
                     STVACVVAARAAQRLDGDVDVLVAPPQSYGASGEHEGFAGTVSVGHDALRLLITEIGRS
                     MLRWAGRLLVVNGHGGNLVSLADAVRGLRDESRDVAWWPCMPPGSDAHAGHTETSMMLR
                     LRSSAVRKERAVAGPTQPVHLLMDRLVTESVRGVSPSGVLGDPAGARASEGERLLDGMA
                     DRLATDIRAWQVSARGRLGQPARAER"
     gene            complement(10001..11173)
                     /gene="mftD"
                     /locus_tag="N6J21_RS37355"
     CDS             complement(10001..11173)
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658591.1"
                     /locus_tag="N6J21_RS37355"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_093720016.1"
                     /sec_met_domain="TIGR03966 (E-value: 5.9e-214, bitscore:
                     708.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKWFETVAEAQRRAGRRLPKSVYGALLAGSEKGTSYDDNTKAFA
                     ELGFAPHVAGLSAKRDQSTTVLGEEISLPVIISPTGVQAVHPDGEVAVARAAAARGTAM
                     GLSSFASKPLTEVVAANPRVFFQMYWMGSKDAMLRRMQYAREAGAKALIVTLDWSFSHG
                     RDWGSPRIPERLTLKEMVRFAPEAVARPRWLLDFVKSGRLPDLTTPNLTEEGQDPPTFF
                     GAYGEWMGTPPPSWEDVAWLRAQWDGPFLLKGVCRVDDALRAVDAGVTAVSVSNHGGNN
                     LDSTPAPIRALPAIADAVGSQVEILLDSGVRRGSDVVKALALGARAVMIGRAYLWGLAA
                     GGQSGVENVLDILRGGIDSALLGLGRSSVRELTRDDVVIPPGFTRTLGAV"
     gene            complement(11170..12405)
                     /gene="mftC"
                     /locus_tag="N6J21_RS37360"
     CDS             complement(11170..12405)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671058.1"
                     /locus_tag="N6J21_RS37360"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_261720491.1"
                     /sec_met_domain="PF04055 (E-value: 3e-25, bitscore: 88.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 2.4e-06, bitscore: 25.9,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 9e-14, bitscore: 50.4,
                     seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 0.00013, bitscore: 21.2,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLHDPPPADRVPALVEQFKGGLNAPICLTWEWTYACNLSCAHC
                     LSSSGRRDPLELSTDEIKSVIDELQRMQVFYVNVGGGEPTVRPDFWELLDYAVGHQVGV
                     KFSTNGLRLTPERARRLAATDYVDIQISLDGATREVNDAVRGSGSYDMAIRAMENLAEA
                     GFGDFKISVVMTRQNVPQLDDFAAIADYYGAQLRITRLRPSGRGADVWDDLHPTSAQQL
                     DIYTWLLAHGERVLTGDSFFHLNALGSDPLPGLNLCGAGRVVCLIDPVGDVYACPFAIH
                     DAFKAGNVRSPGGFDRVWRESALFADLRRPTTAGACSGCPAYDSCQGGCMAAKFFTGLP
                     LDGPDPECVKGHGAAALASVDAGALPRQGKDHSHRTVPLGMPAVRTARPDRLCDESPLA
                     GAPLAQHLKGTS"
     gene            complement(12402..12707)
                     /gene="mftB"
                     /locus_tag="N6J21_RS37365"
     CDS             complement(12402..12707)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076821.1"
                     /locus_tag="N6J21_RS37365"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA.; MftB, a small
                     protein, is a peptide chaperone that assists the radical
                     SAM enzyme MftC in performing two modifications to the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     peptide, MftA. MftB's role is analogous to the role of PqqD
                     in the biosynthesis of PQQ, a cofactor that derives
                     entirely from a Tyr and a Glu in the precursor PqqA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_261720492.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 7e-26,
                     bitscore: 89.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSHAKAAALDLAAPYRLNPSVALRPEPFGALAYHFGNRRLSFLK
                     APELVELVRELDRHPSVGHALAGVPGARRAAFRRALASLAAADMIIPQRSGTGAPP"
     aSDomain        complement(12438..12668)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_N6J21_RS37365_Mycofactocin_RRE.1"
                     /evalue="2.60E-28"
                     /identifier="RREFam016.1"
                     /label="N6J21_RS37365"
                     /locus_tag="N6J21_RS37365"
                     /protein_end="90"
                     /protein_start="13"
                     /score="89.1"
                     /tool="antismash"
                     /translation="APYRLNPSVALRPEPFGALAYHFGNRRLSFLKAPELVELVRELDR
                     HPSVGHALAGVPGARRAAFRRALASLAAADMI"
     gene            complement(12718..12831)
                     /gene="mftA"
                     /locus_tag="N6J21_RS37370"
     CDS             complement(12718..12831)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671060.1"
                     /locus_tag="N6J21_RS37370"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_093720013.1"
                     /transl_table=11
                     /translation="MHEEQITAETDTAHHADELVEEVLVEEVSIDGMCGVY"
     gene            13188..14480
                     /gene="mdo"
                     /locus_tag="N6J21_RS37375"
     CDS             13188..14480
                     /EC_number="1.1.99.37"
                     /EC_number="1.2.98.1"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0046170 - methanol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mdo"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662116.1"
                     /locus_tag="N6J21_RS37375"
                     /note="This methanol dehydrogenase is considered a
                     nicotinoprotein, since its NADP cofactor remains is not
                     dissociable, but instead remains permanently bound. A
                     member of this family has been shown to act as a
                     formaldehyde dismutase, able to convert two molecules of
                     formaldehyde (plus one water molecule) into one of methanol
                     and one of formate, with no net change in its redox state.
                     More recently, it was shown in Mycobacterium smegmatis that
                     this enzyme is critical to ethanol utilization, for which
                     the biosynthesis of the cofactor-like electron carrier
                     mycofactocin is also required.; This methanol dehydrogenase
                     is considered a nicotinoprotein, since its NADP cofactor
                     remains is not dissociable, but instead remains permanently
                     bound. A member of this family has been shown to act as a
                     formaldehyde dismutase, able to convert two molecules of
                     formaldehyde (plus one water molecule) into one of methanol
                     and one of formate, with no net change in its redox state.
                     More recently, it was shown in Mycobacterium smegmatis that
                     this enzyme is critical to ethanol utilization, for which
                     the biosynthesis of the cofactor-like electron carrier
                     mycofactocin is also required; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="NDMA-dependent methanol dehydrogenase"
                     /protein_id="WP_261720493.1"
                     /sec_met_domain="Fe-ADH (E-value: 6.8e-108, bitscore:
                     359.5, seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVEELLKPFPIKEFHPFPRALMGPGAHELIGPEAKKLGFRRTLV
                     MTTGLRGSDTVHKIVESMKYHGLEVVVYDKVESNPKDYNVMDAVALYQENKCDSFVSIG
                     GGSSHDACKGARISVAHDGRSVNDFEGFNKSENPKNPPHIAVSTTAGTGSETSWAYVIT
                     DTTTDPDNPHKYVAFDDASVATLAIDDPTLYYDCPLDYTAQCGFDVLAHASEPYVSRLN
                     FQPSLGNALHAIRLTARHLRQAVWNGQDLAGREGMMYAQYIAAQAFNSGGLGIIHSISH
                     AVSAFYDTHHGLNNAIALPRVWAFNMPAHYERFADIAEAMGVDTHGMTKVQAAEAALAA
                     GIRLLRDVGIPEKFTDVQQDTYSKNRLGQGPTKFYEQAKEIKGDAGDIDRITHHVLGDA
                     CTPGNAKECTFETVRPVVEHCMTGDLDDLLG"
     gene            14558..15727
                     /locus_tag="N6J21_RS37380"
     CDS             14558..15727
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671062.1"
                     /locus_tag="N6J21_RS37380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoxR family ATPase"
                     /protein_id="WP_093720011.1"
                     /transl_table=11
                     /translation="MPATPASHPFGSVDEVTERFAAEGYIADRRLATTVYLQTRLDKPL
                     LLEGPAGVGKTELARTLAAATGRRLLRLQCYEGQDESRALYEWDYGKQLLYTQMLREKI
                     GELTADAPDLASAVHRIEEQDSVFFSDRFLAPRPLLEAVRGEQPAVLLIDEVDRADEAL
                     EAVLLECLGEYQVSVPEIGTYTAKVPPYVVLTSNNTRDLSAALKRRCLHLFLDYPDAGR
                     ELEILRAKDTGLPEAAARHLVDVVRGLRELPLRKSPSISETVDWARTLAVLGVDELDPA
                     ILADTANVVLKYERDLNRALDVLPRLVDPNRTLPEPGHDHHHHHDGHHHDHDHDHEPDG
                     RTVRAAMDRQGRHDEGYYGAPGAGPAHRPATGVGQGARSFAAMARRRTD"
     gene            15741..17270
                     /locus_tag="N6J21_RS37385"
     CDS             15741..17270
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671063.1"
                     /locus_tag="N6J21_RS37385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VWA domain-containing protein"
                     /protein_id="WP_261720494.1"
                     /transl_table=11
                     /translation="MESGVHRFVRVLRLFGLRVSVAEAMDAMRGAAQPGVLADRETLRA
                     ALRMTLVKDRRDHELFDELFTAFFSLRPVGGGADGHGHGHEHDDLTDTGELESFTVSDE
                     PSETPQQGHSHGKPSDIRDFFDQQDLAQQYNLHQEANKIDLASMTDEIVLSKDGATGGR
                     DDTPSVQLETERLHGAGVPGRLAASTGQPIDTALTVAQRDALLGWLADQDDDPEEVGEL
                     RSQLTGIIEDLPELLRRHLDRLAELHSVAVESARIAERARLETVDEDERGQLEEALRRI
                     GRTVHGALTSRRQVRPAGRVHPGRTMRHNMRYDGVPFRPVTVTRAEDRPRLLVLADVSL
                     SVRATARFTLHLVHGLQSLFGQVRSYAFVDEPTEITELFAEHPLERALGLVFDGLPAGG
                     LLDVDATSDYGRTFETLLADHASALGRRTTLLVLGDGRGNGNPPRTETFAEITRRVRQT
                     IWLTPEPRYSWHLGRCDLPAYAEHCDRVQVVADLAGLERTAHRMTTEAVGR"
     gene            17284..18222
                     /gene="mftM"
                     /locus_tag="N6J21_RS37390"
     CDS             17284..18222
                     /codon_start=1
                     /gene="mftM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671064.1"
                     /locus_tag="N6J21_RS37390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mycofactocin oligosaccharide methyltransferase
                     MftM"
                     /protein_id="WP_261720495.1"
                     /transl_table=11
                     /translation="MSVPSLRDAIAARRIDPLAPGTPGLYADHLVRVVREPHPVPSDGP
                     RPIVRTAHFVLRRRGRRVELGHRLRPDQLDNDLAGLLAEELFAPGWLSGSDIFERVFTG
                     VVRSCVDGPLPAWTTFYGNTLTRIRQSFQHPEQADHSSIAGFAPVYRYALDLVGTDSVL
                     DLGSCFGFLPMLLAERESGGSGSGSVIASDLSGGTMRLLGAVAGARGLRLDVLVCDAAQ
                     VPLPDRAVDTVTVIHVLEHLDAEHGGAVLREALRLARRRVVVAVPYEDEPTAAYGHVRC
                     FSPRELTALGRRSGRTFWVAAHHGGWLVVDR"
     gene            complement(18236..18901)
                     /locus_tag="N6J21_RS37395"
     CDS             complement(18236..18901)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015493196.1"
                     /locus_tag="N6J21_RS37395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_261720496.1"
                     /transl_table=11
                     /translation="MTIEQQAGTATDALRIVLVDDHAIVRHGLRSILERESDLEVVGEA
                     STAAEAEAVVERARPAIVLLDLKLSTAADTEGLELCKRLTQRHPNLAVLVLTTFLDDSL
                     VVQAIHHGARGYVVKDVDTTELLRSIRAVARNESAFDSHAAAAMVRSMRVQPERPRFTE
                     RELKVLQLLAHGLSNREIGAELYISETTVKFHVRNIMRKTDAGSRAEVVYEATKLGVI"
     gene            complement(18898..20268)
                     /locus_tag="N6J21_RS37400"
     CDS             complement(18898..20268)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671066.1"
                     /locus_tag="N6J21_RS37400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_261720497.1"
                     /transl_table=11
                     /translation="MSELEPSHHTADLGMLTGLRSGKRSYYPAYVRSTERLERTVEALD
                     GISRALVRTAEGPRALVETVVRTAAEHLRARWLLLAVADGALRAARPRFLLYADDVLID
                     DETRIPAEARYHLELLRTRPWEAQGPGQGRDWVRAPMTLDDEPVGGIAAQPGAGVEVAD
                     TDLAILRVLANQAAVALHHTFLFHAATTLRGRADELSEAAEQQARDLAARSAELAETQA
                     RLLDAMHRQALDDERRRIARELHDSVSQYVLSAGMTLEVCRAELAALGGPAADIAERLA
                     GAKDLNRQAVERLRAAIYALNRTSKEPVGPLPVMLERLSTVHLPTDLDVRVQLAGRPVP
                     LPPQAEQSILRLTGEALFNSATHGNARRALVRLRYLPDAVVLTVSDDGDGEPAQLRRAL
                     RMARVTDLGGRHRGLANMVARAEELAGRLSIRRSKMGGVLLRVDVPLPVRDAVGGTG"
     gene            complement(20279..21553)
                     /locus_tag="N6J21_RS37405"
     CDS             complement(20279..21553)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671067.1"
                     /locus_tag="N6J21_RS37405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_261720498.1"
                     /sec_met_domain="Fe-ADH (E-value: 3e-117, bitscore: 390.3,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLTSEEVVTGGNDPSADWSRDEHQGELLGPQRTSTAAVRESARL
                     AKFHVPEIVFGPGSLTELGHCALRLGGRRPFLVTDPGLMAAGWADEAVAHLRRAGLRPV
                     VWSDITPNPKDHEVQGGYERYVESGCDVIVGVGGGSVIDAAKGVAILSGNGGRILDYEG
                     VDRVVQPIPPTVMVPSTSGTGADVSQFAVITDTTAHIKITIVSRTLVPDISVIDPRLLT
                     TMPPWLNAATGLDALTHAIEAFVSRAHNPLTDMHALHAVELIMGNLLRTQLEPRDFGAR
                     LAMAQAALHAGMAFTNAILGATHAMSHQVGGLLDAPHGVVNGVLLPHVIRFNAEAWPDR
                     FVALGAAAGLPTQGVPAYEVAAGLADLVRALADDVGVPKGLASLGVVEGDVPVLARTTL
                     KDACMATNPRLVELRDVETLFREAL"
     misc_feature    complement(22347..22361)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 23.58"
                     /tool="antismash"
ORIGIN
        1 gcccgggacc gcgtcggcgg ccgggtcgaa cagacccgcg cccccgacgg ccgcaccgtc
       61 cagctcggcg gcgaggtcgt cggccacgcc cacaccgcct accttcgact cgccgcggaa
      121 ctcggcctga agctggtccc cagctacgtc gaggaaccgg gccgcatgac ccgcgccacc
      181 ccggaaggcc gctcgacggg cgaaccgccg cactggttcg gccccggaga cacggccacc
      241 cacgaacggc tgagcaagga gttcgccgcc ctcgccgcca ccgtcgaccc ggacgacccc
      301 tggtcacacc ccgacgccac cgccctcgac cgggtctccg tcgccgactg gctgcgctcc
      361 ggcggggccg gccccgccgt ggtccgcctg tgggacatcg gccaactcgc cctggcgggc
      421 ggctcctacg agcgcatctc cctgctcgcc gcactgcgca agagcgccgc cgtccccggc
      481 tcgggcaccg gcgactacga ctacgaggac tgggaaggcc tgcgcctcgc cgagggctcc
      541 gcgacactcg ccgaagtcct gggccgcgtc ctcggcccgc gcatccgcct cggctcgccc
      601 gtcgccgccg tggacatccg ccccggtatc tgcaccgtgc gcctgcgcac cggcgaggtc
      661 ctcaccgcgg gagccgtggt gagcgccctg cccgtcggac cgctgaggga catcgccgtc
      721 agcggagtca gcgacgagcg cctcgcctcc ctgcaccgcc aacgccaggc cgtcgcagcc
      781 aagttcgccg ccgtctacga ccgccccttc tggcgggacg ccggactgaa cggcctgtcc
      841 gagacggagg gcgtcctggg cagcacctgg ccgcagaacg agggcgtcct gtcggccctg
      901 gtcccacccg agcgctacgg cgtcctcctc ggcaccccgc cccacctgcg cacccccgaa
      961 ctcctcaccg agatcgccgc catgttcggc gacgaggccc tgcggccgac cgcctgccat
     1021 ctgcggctgt ggggcaccga cccgtggacc caggggtacg tcacccagtg gacgcccggc
     1081 gaggtgatgg ccgtcggccc gctgcacggc cgccacgaac cgccgttcta cgtctgcggc
     1141 tccgaccaat gggtcgccgg atacatggag ggcgccgtcc gcaccggccg cgccgcggcc
     1201 gaggaggcgc tgcgccgtgg ctgacagcga ccgactcaac cacatcggcg gcaaggacgt
     1261 tccggccgcg tccggcgcca ccctgctgct gatcgacccg gccaccgggc tgccccacgc
     1321 cacggccccg ctgtccggcc cagaggacgt ggacgcggcc tgccgggccg cggaggacgc
     1381 cttcgagaac tgggcgtcga caacgcccgg tgagcggcag acggcactgc tcaggatcgc
     1441 cgacagcctt gaacggacgg ccgaccggat cgcggacgcc gaagtggccg acacgggcaa
     1501 gccgcggtcc ctgttcctcg ccgacgaact gcccgccatc gtggacgtgt tgcggtacta
     1561 cgccggagcc gcccgcaatc tgcccggctc ggccgccgcc gagtacacac cgggccgcac
     1621 ctcggtcctg cggcgcgaac ccatcggcgt ctgcgcgcag atcaccccgt ggaactaccc
     1681 gctgatgatg gccgtctgga agatcgcccc ggccctcgcg gccggcaaca cggtcgtcct
     1741 caaaccctcc gacaccacac cgtcctcggc cgtcctcctg gcccgactcg ccgccgagca
     1801 cctgccgccc ggcgtgctga acgtggtgtg cggcgaccgc gacaccgggc gggccctggt
     1861 cgcccacccg gacgtgcgcc tcgtcgccgt cacgggaagc gtgcgcgcgg gccaggagat
     1921 cgccgcggcg gcagcggcgg acctcaagcg cctgcacctg gaactcggcg ggaacgcgcc
     1981 cgtgttgatc cacgcggacg ccgatctcga cgacgcggtg gagcagttgg cgtcactgtc
     2041 gttctacaac gcgggccagg actgcacggc cgcgaccagg atcctggtcg accggcgtat
     2101 ccacgacacc ttcctgaccc ggttcacgca gcgggtggcg cgtctgcgcc ccggggcccc
     2161 ggacgacggg cacgcggact tcggcccgct ggccaacgcg gcccaactcg ccgccgtacg
     2221 cggcctgctg gagcgtctgc cggcgcacgg ggagatcgtc tcgggcggtg cggtggtgga
     2281 ccggcccggc tacttccacg agcccacggt agtcgccgga gtgcggcagc tggacgagat
     2341 cgtgcagcgc gaggtgttcg ggccggtcgt caccgtccag cccttcaccg acgaggccga
     2401 ggccgtacgg ctggccaacg gcgtcccgca ggggctcgcc gcgagcgtgt ggacacgcga
     2461 ccacgaccgg gccatgcgcg cgacacgcgc cctgcgcacc ggcatcgtct gggtcaacac
     2521 ccacggcacg acggtctcgg agatgccgca cggaggggtg ggccactccg gctgcggcag
     2581 cgatctctcg ctcaccggac tgctcgacta cacgcaggtc aagcacgtca tgctgtgacg
     2641 cgatcggggt gcggcgaccg cgagcattgc ggaccgaagc tgtccgcaac gcctcctacg
     2701 gtcgtcccat gacccagacg cagaacatcc ttgtcaccgg tgccaccgga accgtcggcc
     2761 gtcaggtcgt cgccgaactc ctcacccgcg gccaccgggt ccgcgcactg acccggaacg
     2821 cggcgaaggc cgacctcccg gccggggtcg aggtggtcga gggcgacctg acggacccgg
     2881 acaccctgac cccggccctg gagggcgtga ccggcctgca cctgatcacc ttcggtggcg
     2941 cgtacttcgc cccgctgcag acgggcccga ggattcttga gctggcccgc gcggccgggg
     3001 tacgccgggt caccgtgctg cacggcggcg aggggacccc gctggaggac gccgtgcgcg
     3061 ccgacgacgg cgtggactgg accgtactca tgccggtcga gttcatggcc aacgcgttgg
     3121 agtgggcgga cggcatcgtc acccgggacg aggtccggga gccgttcgtc tcccgcctga
     3181 gtgccatggt ccacgagggc gacatcggcg ccgtggccgc ggtcgcgctc accgaggagg
     3241 gccacggcgg acaggagtac gtgatcaccg gccccgaagt gctgaccgtc gcggacaagg
     3301 tggccgccgt cgcgggcgcg cgggggcggg cggtgtccct cgtcgaactg accgaggagc
     3361 aggccgtcga gcagtggcgg gcagcgggcc acaccgacga ggtgatcggc ttcctgctcg
     3421 aagccttcgg aaacaccccg gagatcggcc gcacggtcac cgacaccgtc gagaaggtca
     3481 cgggccgccc cgcgcggacc ttccgccagt gggcgcagga gcacgcggag gccttcgggc
     3541 cacacgcgtg acgaggtccg cgcgacgcgg acaccctggg cggcacctga acgtcccgcc
     3601 caggggacac ctcgctcaca cgcgcggcgc cgacaggcct tccagggccc gtgccgtgat
     3661 ctcgggcacc ggcccgtccg cggggaccgt cacgaggagg tccgcgtagt acgcgagcag
     3721 cggcgcggtc tgcttctcgt acacccgctg ccggttgcgg atgatctcct cggtgtcgtc
     3781 cgcccggccg cgccccagca gccgcgcgac gacgacctcc tcggggaccg agagctccag
     3841 caccgcgtcg agtgccgtgc cggtggcggt gagcatcgcg gcgagttcct gcgcctgagg
     3901 caccgtgcgg gggaagccgt cgaggaggta gccgtcgcgg gcgtccggga gtgcgaggcg
     3961 ttcgcgcacc atgccggcgg tgacgtggtc ggggacgagt tccccggtgt caaggtagcg
     4021 ctgggcttcc tggccgagcg gggtccggcg tgcgacatgg tcccggaaca ggtctccggt
     4081 ggagatgtgg gggacgccga gccgctcgct caaggcgacg gcctgggtgc ccttgcccgc
     4141 tccgggaggc ccgatgagga tgattcgcat cagcgatgcg cacctttccg tgagagctgt
     4201 tcgtcggtgg cccgttcggc cgattgcttg atcgcaatat ccgctgtcgg actcgcggtt
     4261 cggtcggttc cctctggcag cacggtggtc aggcggcccg ggaggcgcag tcgggttcgg
     4321 cgctgaggac cagcaccggg ccctgggccg gatccatccg gtgggtcagc agatcgcaca
     4381 atacttcggc caggccgacg aggtgcgccg ccgcgtccag cgacgggctg gggatcagcg
     4441 tatgtacgtc cacccggggt gccagctcgg cggccagcga ctcggtgagt tcggtcagcc
     4501 cggccagcgt ggcgtcccgg gcgccgccgc gccccgtcgc cgcgaccacg ttgaccaccg
     4561 atccgccgtg cgccgccatc caacgccggt aggcgcgctg gacgaagctc agcggcatca
     4621 ccaggtcccg ctggaacgtt tcgcgcagat ccagcggatc cagttccatc agcggtcccg
     4681 tgcgggggcg gcccgcgacc aggttcacca cgtggtccaa acggccgagc cgctccacca
     4741 ccgtgtcgac gacccggccg ggtccggcag cgtccgacgg ggtgaagggc agcgcgagca
     4801 cggaggaacc ccgcggaacg ggcgcgccgg cgactgccac ccgcgccccg cgtgccacga
     4861 ggctcgccgt caccgccggg gccggctcgc cctccccggt gacgaggacg acccggccgg
     4921 tcaacgtact gatgtgtccc atgaccgcac accctgccga tcagccggac tgctcggacg
     4981 cgtcggccag accggcgtgg atgcggtcca tgaccgaggc gtccgcgagc gtggacacat
     5041 caccgacctg ccggttctcc gcgacatccc gcaacagccg ccgcatgatc ttgcccgagc
     5101 gggtcttggg cagctcgggg acgaccagga tccgccgcgg cttggcgatg ggcccgatct
     5161 cccgcgccac gtgctcgcgc agggcgtcca ccgcgacctc gcccccggca ccctcgcgca
     5221 ggatgacgaa ggccacgatc gcctgccccg tcgtcgcgtc cgaggcaccg accaccgccg
     5281 cctcggccac cgccgggtgg gagacgagcg ccgactccac ctcggaggtg gagatccggt
     5341 gccccgacac gttcatcacg tcgtcgaccc ggcccagcag ccagatgtcg ccgtcgtcgt
     5401 cgtagcgggc gccgtccccg gcgaagtagt agcgctgcgc cgcgaacctc gcccagtacg
     5461 tctgccggta gcggtcgtcg tcgccccaca ccccgcgcag catcgacggc cacggctggt
     5521 ccagcaccag atagccgctc tcgcccttcg cgacctccac gccctggtcg tcgaccacct
     5581 tcgcggagat tcccggcacc ggcgtctgcg ccgaaccggg cttgaaggaa ggggagtcgg
     5641 gcgacggcgc gatcatcacg gcgcccgtct ccgtctgcca ccaggtgtcc acgaccggcg
     5701 cccgcccggc cccgatgtgc tcgcggtacc accgccacgc ctccgggttg atcggctcac
     5761 ccacactgcc gagcacccgg atcgaggaca ggtcgtggcg ggccgggatg tcctcgcccc
     5821 acttcatgaa cgtccggatg agcgtgggcg ccgtgtagta cacggacacc ccgtacttct
     5881 cgatgatctc gaagtgccgc ccctcgtgcg gggtgttggg cgtgccctcg tacagcaccg
     5941 atgtcgtgcg gttggcgagc gggccgtaga cgatgtacga gtgcccggtg atccagccga
     6001 tgtcggcggt gcaccagtac acgtcggtgt ccgggtccag cccgaacacc gtgcggtgtg
     6061 tgcaggcggc ctgcgtgaga tagccgccgg aggtgtgcag gatgcccttc ggcctacccg
     6121 tagtgcccga ggtgtagagg atgaacagcg ggtgctcggc gtcgaacgcc tccggctcgt
     6181 gccggtcgga ctggacgtcc accagctcgt gccaccacag gtcgcggcct tccgtccacg
     6241 gcacgtcggt gccggtgcgc cggaccacca ggacatgctc cacgcccggc gtgtcgcggg
     6301 tcgcctcgtc ggtgttcgcc ttcatcggct cgaccctgcc ccggcggtac tggccgtccg
     6361 aggtgatcag cagccgcgcc ccggcgtcct cgatccggga ctgcagcgcg gccggtgaga
     6421 acccgccgaa gaccacgctg tgcggcagcc ccagccgggc gcacgccagc atcgcgaaca
     6481 ccgcctccgg gatcatcggc aactggatcg ccacccggtc accggcccgc agaccgagcg
     6541 acagcagcgc gttcgccgta cggcagacct cgcgccgcag ctccgcgtac gtgatcgtgc
     6601 gggtgtcgcc cggctcgccc tcccagtgga aggccacctg atcgccgtgg ccggccgcca
     6661 catgccggtc gacgcagttg tacgccacgt tcagcttccc gccggtgaac cagcgggcga
     6721 acggggcgtc cgaccagtcc aggacctcgc tccactcggt gtcccagtcc agctcgcccg
     6781 cctgccgcgc ccagaacgcg aggcggtcgt ccacgggctg cgcggtctgg ccgtgacggc
     6841 tgtggtggtc ggaacggggc gacatacggt tcctccagtg aggtgcgggt gcggccgacg
     6901 gccccgctga gggcggcggg accgtctccg agagccacag tgggcggaca gggttggcgg
     6961 caggttggcg gcgccgaggc ccttcgttcc gatcagcccg gctgcggccg ggcgccggtc
     7021 cagcggctca ccatgcgcag ggcgagccgg ggccgcagcg gggcggtggt gcggtggcgc
     7081 acggcgccct gccagacgcc ccagccgtag gccaggtcgt ccaggcggcg cagcgcgaga
     7141 tgggtgagcg gtgggagcgg gctgccggag cggcggtggt cgagcagtcc gtcggcgacg
     7201 gcggcggtga gcaggagcca tcgggcccgc ttgtccacgg cggccgcggc gagcgcgacc
     7261 ggccagtggt ggcgggtggc gcagcgcagc aactgttccg cggtgccccg cacggcggcg
     7321 acggacagca gggcgcccgc gcgtgccggg tggtcggcgt cgggcatccg gcgcgccacc
     7381 cgtacggccg tcaccccggt cagtacggcc gcggcggcca ccggcacggg cctgccccgc
     7441 agcagcaggg cacaggcggt cagcgaccag ggcgccgcgt acagcggcgg cacctgcccg
     7501 gggtgccgca gcgccagggc ggcggccccg gtgccgtaac tcgcgcgctg cgccagccag
     7561 ttgcgcaggt ccgtgcggtg gcgatggccg accctcgcgg aaggtacgta ccgcacacgc
     7621 cagcccgcct cgtgcaaccg catgcacagg tcgacgtcct caccgacccg cagagccgtg
     7681 tcgaacccgg cgcccacggc cgccctgcgc accacgatcg tcgcgctcgg cacgtacgac
     7741 agcgccgaca ccggcaccac cggcccctcc gccgcgccca tgtccagcgg cgagcgcagc
     7801 ttctcgtacc ggtccagcag ccccggcgac ggcaccggca acgccaccac acgcggcgcg
     7861 acgagcgcca ccgccggatc ggtgaactgc gccaacagcg gcgccagcca gcccggttcg
     7921 ggcaccacgt ccgagtcgca gaacgccacg aatgcggtac gacagtgccg cagcccggtg
     7981 ttgcgggcgg cggccgggcc ctggttgtac gggtgcgtga tcagccgggc gccgtgcctg
     8041 cgagccacgt ccgccacggc ggcgggggag tgggaggcgt cgtcgacgac cagcaccggc
     8101 agaccggtgg tggccgggtc gcggcgcagg gcgtcgagca gccggtcgag ttggtcggcg
     8161 cggtcacgga cggggatgac gacggtggtg tcccgcaccg gtaccggagc ggggcgcggg
     8221 tgggcgatcc cggcgtccag caggcgccgg gcgagcgcgg cgctggggcc gtccgtcacg
     8281 gagaaccggc cgtcgccaag gcgccgtacc gccgcctcgg tcagcctcat cagtcgcaac
     8341 ggcgagccgc ccatcatcag ccgcccgtcc gccgagcggt gagagccggg ggcgagttcg
     8401 acggtgaacc cgtcggggag ccggtcaccg gccgggggag cgaggaggtc cggtggggcg
     8461 ggtgccagtg tcatgaggtg aatcctccgt ccgcgtgcag tacggagccg gtgacggaga
     8521 ccgagtcggc cgagcaggcc caggcgacca cggcggccac ctcctccggt tccagggggc
     8581 gttcggtgag ctgccggccg gcgagtgtgt cgacgtcggt gaggccgtac aggccggcgg
     8641 tggcccggag catggcggtg cgggtggagc cgggggacac ggcgagggcg ttcacgccgg
     8701 tgccgcgcag gtcgcaggcg aggccgcgga ccaggccgac gacggcgtgc ttggcggcac
     8761 agtacgcgcc gagccgccac aggccgcggt gcgcggcggc cgaggccagc gccacgaaac
     8821 ggccgctgcg gggctccggg cggcgcagca gcgcgggtac ggcggttcgg gcgaggtggg
     8881 cgacgcccag gacgtccacg tcgagcaggg ttcgccactg ggcgtcggtc tgctcccaca
     8941 cgggggcgcc gccgaggatg acggcggccc cggcgacggc ggcgtcgagt ccgccgaact
     9001 cgcgctcggc gaggtcgacg gcggtgctca gcgcctgctc gtcgcgcacg tccgcccgga
     9061 ccgcgcgtac cagctcgaac tcctcggcga gggcatcgag ttggcccggt tcggccagcg
     9121 gatacggcac ctcggggtcg tccgcgcagc ggtccacggc gaccaccgcc catccgctcc
     9181 cggccagccg ccgcacggtc gctgccccga tcccgcgcgc ggcgccggtg accacggcga
     9241 ccctggggcc ggtcatcgtt cggccctcgc gggctgcccg agccggcccc gcgccgacac
     9301 ctgccaggcg cggatgtcgg tggcgagccg gtccgccatg ccgtcgagaa gccgttcgcc
     9361 ctcgctcgcg cgggccccgg ccggatcgcc gagcacaccg gacgggctca ccccgcgcac
     9421 ggactcggtg accagccggt ccatcagcag atggacgggc tgggtcggcc cggccacggc
     9481 tcgttccttc cgcaccgcgg acgaccgcag ccgcagcatc atcgaggtct cggtgtggcc
     9541 cgcgtgggcg tcggagcccg gcggcataca cggccaccac gccacgtccc ggctctcgtc
     9601 gcgcagcccg cgcaccgcgt cggccaggct caccaggttg ccgccgtgcc cgttgaccac
     9661 gagcaggcgg cccgcccagc gcagcatgga gcggccgatc tccgtgatca gcagccgtag
     9721 cgcgtcatgg ccgacggaca ccgtgcccgc gaacccctcg tgctcaccgc tcgccccgta
     9781 cgactgcggc ggcgcgacca gcacgtccac gtcgccgtcc agccgctggg cggcacgcgc
     9841 cgcgaccacg caggccacgg tggagtcggt gtccaacggc aggtgcgggc cgtgctgttc
     9901 gcaggagccc agcggcagca gcaccagcgg ggcccggccg gtcaggtccg gccaggcgag
     9961 atcggcgagc cggggcgccg gggccggtcc gggcgtcatc tcacaccgcg cccagggtcc
    10021 gggtgaagcc aggcgggatc accacgtcgt cgcgggtcag ttcccgcacg gaggagcggc
    10081 cgaggcccag cagtgccgag tcgattccgc cgcgcagaat gtcgagtacg ttctccaccc
    10141 cgctctgccc gcccgcggcc agcccccata ggtaggcgcg gccgatcatc acggctcggg
    10201 cacccagcgc cagagccttc accacgtcgc tgccccggcg caccccgctg tccagcagga
    10261 tctccacctg ggagccgacg gcgtccgcga tcgcgggcag cgcgcggatg ggggccgggg
    10321 tgctgtcgag gttgttgccg ccgtggttgg acacggagac ggcggtgacc ccggcgtcga
    10381 ccgcgcgcag ggcgtcgtcg acccggcaca cgcccttgag caggaacggc ccgtcccact
    10441 gggcgcgcag ccaggccacg tcctcccagg acgggggagg ggtgcccatc cactcgccgt
    10501 acgcgccgaa gaacgtcggc gggtcctggc cctcctcggt gagattgggc gtggtcaggt
    10561 cgggcagccg cccggacttg acgaagtcga gcagccagcg cggacgggcg acggcctcgg
    10621 gggcgaagcg caccatctcc ttgagggtca gccgctcggg aatgcggggg ctgccccagt
    10681 cacggccgtg cgagaacgac cagtcgaggg tgacgatcag cgccttggcg cccgcctccc
    10741 gcgcgtactg catacggcgc agcatcgcgt ccttggagcc catccagtac atctggaaga
    10801 agacccgcgg attggccgcc accacctccg tcagcggctt gctcgcgaac gagctgaggc
    10861 ccatcgcggt accgcgtgcc gcggcggcgc gggcgaccgc cacctcgccg tcggggtgca
    10921 cggcctgcac cccggtcggg gagatgatca ccggcaggga gatctcctcg ccgagcacgg
    10981 tcgtcgactg gtcgcgcttg gccgacagcc cggcgacgtg cggggcgaag cccagctccg
    11041 cgaacgcctt ggtgttgtcg tcgtaggacg tgcccttctc ggaaccggcg agcagggcgc
    11101 cgtacaccga cttcggcagt cgcctgcccg ccctgcgctg ggcctcggcg accgtctcga
    11161 accacttgct catgacgttc ctttcaggtg ctgcgcgagg ggggcaccgg ccagcgggct
    11221 ctcgtcgcag aggcggtcgg gccgcgcggt gcgcaccgcg ggcatgccga ggggaacggt
    11281 ccggtgggaa tggtccttgc cctggcgcgg cagcgcgccg gcgtccaccg aggcgagcgc
    11341 ggcggcgccg tggcccttga cgcactcggg gtcgggtccg tccagcggca ggccggtgaa
    11401 gaacttggcg gccatgcagc cgccctggca ggagtcgtac gccggacagc ccgagcaggc
    11461 gcccgcggtg gtgggtctgc gcaggtcggc gaagagcgct gactcccgcc acacccggtc
    11521 gaagccgccg ggggagcgga cgttgcccgc cttgaacgcg tcgtgaatgg caaaggggca
    11581 ggcgtacaca tcacccaccg ggtcgatcag gcacaccacg cgtccggcgc cgcacaggtt
    11641 caggccgggc agcggatcgg agccgagcgc gttcaggtgg aagaaggagt caccggtgag
    11701 cacgcgctcg ccgtgcgcca gcagccaggt gtagatgtcg agctgctgcg cggaggtggg
    11761 gtgcaggtcg tcccacacat cggcaccccg cccggacggc cgcagccggg tgatgcgcaa
    11821 ctgggcaccg tagtagtcgg cgatcgcggc gaagtcgtcg agctgaggca cgttctgacg
    11881 ggtcatcacc acggagatct tgaagtcgcc gaagccggcc tccgccaggt tctccatggc
    11941 gcggatcgcc atgtcgtacg aaccggagcc gcgtaccgcg tcgttgacct cacgggtggc
    12001 gccgtccagg gagatctgga tgtcgacgta gtcggtcgcg gcgagtctgc gggcccgctc
    12061 gggggtcagg cgcagcccgt tggtggagaa cttgacgccg acctggtggc cgacggcgta
    12121 gtcgagcagt tcccagaagt cggggcggac ggtgggttcg ccgccgccca cgttcacgta
    12181 gaagacctgc atgcgctgga gttcgtcgat cacggacttg atctcgtccg tggacagctc
    12241 cagcgggtcg cgccgcccgg aggaggacag gcagtgcgcg caggacaggt tgcacgcgta
    12301 cgtccactcc caggtcaggc agatcggggc gttgaggccg cccttgaact gctccacgag
    12361 agcgggcacg cgatcggcgg gcgggggatc gtgcagggtg gtcatggcgg cgctccggtt
    12421 ccactgcgct gggggatgat catgtcggcg gcggccaggg aggccagggc gcgccggaag
    12481 gcggcccgcc gggcgccggg cacaccggcc agggcgtgcc cgacgctggg atggcggtcg
    12541 agttcccgta ccagctcgac gagttcgggt gccttcagga aggacagccg gcggttgccg
    12601 aagtggtagg cgagcgcccc gaacggctcg gggcgcagcg ccacggaggg gttcaaccgg
    12661 tacggcgcgg ccaggtcgag agcggcggcc ttcgcgtggc tggacatggg gccggggcta
    12721 gtagacgccg cacatcccgt cgatggagac ctcttccacg aggacctcct cgacgagttc
    12781 gtcggcgtgg tgcgcggtgt ccgtctccgc agtgatctgt tcctcgtgca tggcgagcct
    12841 ccaagggagt ggggtgcggg gaacgccggt tcgccgaccg gccgttgtcg acgctacgag
    12901 cggcccgccg cgccgctcac cctgcatccg gcccccctcg acctgaccgg tcggacaggt
    12961 gcgcccccgc caccgccggg gccctctccg atcggtcagg ccgggccccc tccgaccgcg
    13021 ccccgctgcc gtccgagcgg ccggtgtcgc cgcccggcgc attcccggag ggtgaggcac
    13081 gccaccaaca ccggcaccgt ccgcgctcca cccggaccct gcgccgccgg cgagggagca
    13141 gccggacacc gtacgcgcct cgttgcgcgg agaggaatgt cgcgatcatg caggtcgaag
    13201 aacttctcaa gcccttcccg atcaaggagt tccacccctt cccccgggcg ctgatgggtc
    13261 cgggcgccca cgagctgatc ggccccgagg ccaagaagct gggattccgg cgcaccctgg
    13321 tgatgaccac cgggctgcgc ggcagcgaca ccgtgcacaa gatcgtcgag tcgatgaagt
    13381 accacgggct cgaagtcgtc gtgtacgaca aggtcgagtc caaccccaag gactacaacg
    13441 tcatggacgc cgtggcgctg taccaggaga acaagtgcga ctccttcgtg tcgatcggcg
    13501 gcggctcgtc gcacgacgcg tgcaagggcg cccggatctc cgtggcccac gacgggcgca
    13561 gcgtcaatga cttcgaaggc ttcaacaagt ccgagaaccc caagaacccg ccgcacatcg
    13621 ccgtctccac cacggccgga acgggttcgg agacgtcctg ggcgtacgtc atcaccgaca
    13681 ccaccaccga ccccgacaac ccgcacaagt acgtggcgtt cgacgacgcc tcggtggcca
    13741 ccctcgccat cgacgacccg acgctgtact acgactgccc gctggactac accgcccagt
    13801 gcggcttcga cgtcctcgcc cacgcctccg agccgtacgt ctcccggctc aacttccagc
    13861 cctcgctggg caacgcgctg cacgccatcc gcctcaccgc ccgccatctg cgccaagcgg
    13921 tgtggaacgg ccaggacctg gccggccgcg agggcatgat gtacgcccag tacatcgccg
    13981 cgcaggcgtt caactccggc ggactgggca tcatccactc catttcgcac gcggtcagcg
    14041 ccttctacga cacccaccac ggcctcaaca acgccatcgc gctgccccgc gtctgggcgt
    14101 tcaacatgcc cgcccactac gagcggttcg ccgacatcgc cgaggcgatg ggcgtcgaca
    14161 cgcacggcat gaccaaggtg caggccgccg aggccgcgct cgccgcgggc atccggctgc
    14221 tgcgcgacgt cggcatcccg gagaagttca ccgacgtcca gcaggacacc tactccaaga
    14281 accgcctcgg ccagggcccg acgaagttct acgagcaggc caaggagatc aagggcgacg
    14341 cgggggacat cgaccgcatc acccaccacg tcctcggcga cgcctgcacc ccgggcaacg
    14401 ccaaggagtg cacgttcgag acggtccggc cggtcgtgga gcactgcatg accggcgacc
    14461 tggacgacct gctcggctga gcaccgcgta ctcgggagcg gggggacgca ccgtgggagg
    14521 tcatcccccc gctcccgccc cgtcggacgg aggaaccgtg cccgccacac ccgcaagcca
    14581 ccccttcggt tcggtcgacg aggtcaccga gcggttcgcc gccgagggct acatcgccga
    14641 ccgacgcctc gccaccaccg tgtacctcca gacccgcctc gacaagccgc tcctcctgga
    14701 gggcccggca ggcgtcggca agaccgaact cgcccgcacc ctcgccgccg ccaccggccg
    14761 ccgcctgctg cgcctccagt gctacgaggg ccaggacgag agccgcgcac tgtacgagtg
    14821 ggactacggc aagcagttgc tgtacaccca gatgctgcgg gagaagatcg gcgaactcac
    14881 cgccgacgcc cccgacctgg cctccgccgt gcaccgcatc gaggaacagg acagcgtctt
    14941 cttctccgac cggttcctcg ccccccgccc gctgctggag gccgtgcgcg gcgagcagcc
    15001 ggcggtgctg ctgatcgacg aggtcgaccg ggccgacgag gcgctggagg ccgtcttgct
    15061 ggagtgcctg ggcgagtacc aggtgtcggt accggagatc ggcacctaca ccgcgaaggt
    15121 tcccccgtac gtggtgctca cctccaacaa cacccgcgat ctgtcggccg cgctgaagcg
    15181 ccgctgcctg cacctgttcc tggactaccc ggacgccggg cgggagttgg agatcctgcg
    15241 ggccaaggac accggacttc ccgaggccgc cgcccgccac ctggtggacg tcgtgcgcgg
    15301 gctgcgcgaa ctgccgctcc gcaagagccc cagcatctcc gagaccgtcg actgggcccg
    15361 caccctcgcc gtgctcggcg tcgacgaact cgacccggcg atcctcgccg acaccgccaa
    15421 cgtggtcctg aagtacgaac gcgacctcaa ccgggcgctg gacgtactgc cccgcctggt
    15481 cgaccccaac cgcaccctgc ccgaaccggg ccacgaccac catcaccacc acgacgggca
    15541 ccaccacgat cacgatcatg accacgagcc ggacggccgc acggtccgcg ccgccatgga
    15601 ccggcagggc cgccacgacg agggctacta cggcgccccc ggcgcggggc ccgcccaccg
    15661 tcccgcgacc ggtgtggggc agggcgcccg ctcgttcgcc gccatggcgc ggcgccgcac
    15721 cgactgaccg ggaggcgtgc atggaatccg gggtgcaccg attcgtccgc gtactcaggc
    15781 tgttcggcct gcgtgtctcc gtggcggagg cgatggacgc catgcgcggc gccgcccagc
    15841 ccggtgtgct cgcggaccgc gagacactgc gcgcggccct gcgcatgacg ctggtgaagg
    15901 accgccgcga ccacgaactc ttcgacgagc tgttcacggc gttcttctcc ctgcgcccgg
    15961 tcggcggcgg cgccgacggc cacgggcacg gccacgagca cgacgacctg accgacaccg
    16021 gcgagctgga gtcgttcacc gtctccgacg agccctccga gaccccgcag cagggccaca
    16081 gccacggcaa gccgtccgac atccgcgact tcttcgacca gcaggacctg gcccagcagt
    16141 acaacctgca ccaggaggcc aacaagatcg acctggcgtc gatgaccgac gagatcgtgc
    16201 tgtccaagga cggcgccacg ggcggacgtg acgacacccc gtccgtgcaa ctggagaccg
    16261 agcggctgca cggcgccggt gtccccggac gcctcgccgc ctcgaccgga cagccgatcg
    16321 acaccgccct gaccgtcgcc cagcgcgacg ccctgctcgg ctggctcgcc gaccaggacg
    16381 acgaccccga ggaagtcggc gagctgcgga gccagttgac ggggatcatc gaggacctgc
    16441 cggagctgct ccgccgtcac ctcgaccggc tggccgaact ccactcggtg gccgtggaat
    16501 ccgcccggat cgccgaacgc gcccgcctgg agaccgtcga cgaggacgag cgcggccaac
    16561 tggaggaggc gctgcgcagg atcgggcgca cggtccacgg cgccctcacc agccgcagac
    16621 aggtcaggcc cgccggacgt gtccacccgg gccgcaccat gcggcacaac atgcgctacg
    16681 acggcgtgcc cttccggccc gtcaccgtca cccgcgccga ggaccggccc cgcctgctgg
    16741 tcctcgccga tgtctccctg tccgtgcggg ccaccgcccg cttcaccctg cacctcgtgc
    16801 acggcctgca gtctctgttc ggacaggtcc gctcgtacgc cttcgtcgac gaacccaccg
    16861 agatcaccga actgttcgcc gagcacccac tggaacgggc cctcggcctg gtcttcgacg
    16921 ggctcccggc cggcggcctg ctggacgtgg acgccacctc cgactacggc cgcaccttcg
    16981 agaccctgct cgccgaccac gcctcggcgc tcggccgccg caccaccctg ctggtgctgg
    17041 gcgacggccg cggcaacggc aacccgccac gcaccgagac cttcgccgag atcacccgcc
    17101 gggtccgcca gaccatctgg ctgacccccg aaccccgcta ctcctggcac ctgggccgct
    17161 gcgacctgcc cgcctacgcc gaacactgcg accgcgtaca ggtcgtcgcc gacctggccg
    17221 gactggaacg caccgcccac cgcatgacca cggaggccgt gggccgatga agggggagtc
    17281 gtcgtgagcg tcccgagcct gcgcgacgcg atcgccgcgc gccgcatcga cccgctggcg
    17341 cccggcacgc ccggcctgta cgccgaccac ctggtccggg tcgtccgcga gccgcacccg
    17401 gtgccgtcgg acggaccgcg gccgatcgtc cgcaccgccc acttcgtcct gcggcgccgg
    17461 ggccgccgcg tcgaactggg ccaccgcctg cgccccgacc agctcgacaa cgacctcgcc
    17521 ggactcctcg ccgaggaact cttcgccccc ggctggctct ccggcagcga catcttcgag
    17581 cgggtcttca ccggcgtggt ccgctcctgc gtcgacggcc cccttcccgc ctggaccacc
    17641 ttctacggca acaccctgac ccgcatccgc cagtccttcc agcaccccga gcaggccgac
    17701 cactcctcga tcgccgggtt cgcccccgtg taccggtacg ccctggacct cgtcggcacc
    17761 gactcggtgc tggacctggg ctcctgcttc ggcttcctgc ccatgctgct ggccgagcgc
    17821 gagtcagggg ggtcggggtc ggggagcgtc atcgcctccg acctctccgg tggcaccatg
    17881 cgcctgctcg gcgccgtggc cggggcccgg ggcctgcggc tggacgtgct ggtctgcgac
    17941 gcggcccagg tgccgctgcc ggaccgtgcg gtcgacacgg tcaccgtcat ccatgtgctg
    18001 gagcacctcg acgccgagca cggcggcgcc gtcctgcggg aggcgctgcg gctggcacgg
    18061 cggcgcgtgg tggtggcggt gccgtacgag gacgagccga ccgccgcgta cgggcatgtg
    18121 cggtgcttca gcccgcggga gctgaccgct ctgggccgcc gcagcggccg taccttttgg
    18181 gtcgccgccc accacggcgg ctggctcgtc gtcgaccgct gacttccccg ccccgtcaga
    18241 tcacgcccaa cttggtcgcc tcgtacacca cttcggcgcg gctgccggcg tccgtcttgc
    18301 gcatgatgtt gcggacgtgg aacttcacgg tcgtctcgga gatgtacaac tccgcgccga
    18361 tctcccggtt gctgagcccg tgtgccagca actgcaggac cttcagctcc cgttcggtga
    18421 acctgggccg ctcgggctgg acccgcatgg agcgcaccat cgcggcggcc gcgtgcgagt
    18481 cgaaggcgct ctcgttgcgg gccaccgcgc ggatggagcg cagcagttcc gtggtgtcga
    18541 cgtccttgac cacatagccg cgcgccccgt ggtggatggc ctgcaccacc agcgagtcgt
    18601 cgaggaaggt ggtcagcacc agcaccgcca ggttcgggtg gcgctgcgtc agccgcttgc
    18661 acagctccag gccctcggtg tccgcggccg tggacagctt caggtcgagc agcacgatcg
    18721 ccggacgcgc ccgctccacg acggcctccg cctcggcggc cgtgctcgcc tccccgacca
    18781 cctccagatc gctctcgcgc tccaggatgg accgcagccc gtgccggacg atggcatggt
    18841 cgtcgaccag cacgatccgc agcgcgtcgg tggccgtccc ggcctgctgt tcgatggtca
    18901 tccggtgcct cctacggcgt ctctcaccgg cagcggaacg tcgacccgca acaggacgcc
    18961 ccccatcttc gaccggcgga tggacagtct tccggcgagc tcctccgcgc gcgcgaccat
    19021 gttcgccaga ccgcggtggc gcccgcccag gtcggtgacc ctggccatgc gcagggcgcg
    19081 gcgcagctga gccggttcgc cgtcaccgtc gtcggagacg gtcagcacga ccgcgtcggg
    19141 cagatagcgc agccggacca gggcccggcg ggcgttgccg tgggtggcgg agttgaacag
    19201 ggcctcgccg gtcagccgca ggatcgactg ctccgcctgg ggcggcagcg gcaccggacg
    19261 gcccgccaac tgcacccgta cgtccaggtc ggtcggcaga tgcaccgtgg acagccgctc
    19321 cagcatcacc ggcagcggcc cgaccggctc cttggaggtc cggttcaggg cgtagatcgc
    19381 ggcgcgcagc cgctccaccg cctgccggtt gagatccttc gccccggcca gccgttccgc
    19441 gatgtccgcg gcgggcccgc cgagggccgc cagttccgcc cggcacacct ccagcgtcat
    19501 gcccgcgctc agcacgtact gcgagacgct gtcgtgcaac tcccgtgcga tacggcgccg
    19561 ttcgtcgtcc agcgcctgcc ggtgcatggc gtccagcagc cgcgcctgcg tctcggccag
    19621 ctcggcgctg cgcgcggcca ggtcccgggc ctgctgctcg gccgcctcgg acagttcgtc
    19681 ggcccggccg cgcagcgtgg tcgccgcgtg gaacaggaag gtgtggtgca gtgccacggc
    19741 ggcctggttg gcgagcaccc gcaggatcgc cagatccgtg tccgcgacct cgacaccggc
    19801 gccgggctgg gcggcgatac cgccgaccgg ttcgtcgtcc agtgtcatcg gcgcccgcac
    19861 ccagtcacgg ccctgcccgg gcccctgcgc ctcccagggc cgggtccgca gcagctccag
    19921 gtggtagcgc gcctcggccg gtatccgggt ctcgtcgtcg atgagcacgt cgtccgcgta
    19981 gagcaggaac cggggccgcg cggcgcgcag tgcgccgtcc gccaccgcca gcagcagcca
    20041 gcgggcccgc agatgctccg ccgcggtccg tacgaccgtc tccaccagcg cgcgcggccc
    20101 ctccgcggtg cgcaccaggg cgcgggatat gccgtcgagg gcctccaccg tgcgctccag
    20161 acgctcggtg gagcgcacgt atgccgggta gtacgaccgt ttgcccgacc gcagcccggt
    20221 gagcatgccc aggtcggcgg tgtggtgcga cggttcgagc tcgctcacca ggaccgcctc
    20281 acagcgcttc ccggaacagc gtctccacgt cccgcagttc cacgagcctg gggttggtcg
    20341 ccatgcaggc gtccttcaac gtcgtacgcg ccagcaccgg cacatcgccc tcgacgactc
    20401 cgaggctggc cagccccttc ggcaccccga catcgtccgc cagcgcccgc accagatccg
    20461 cgagcccggc cgccacctcg tacgcgggca ctccctgggt cggcagtccg gccgccgcgc
    20521 cgagcgccac gaaccggtcc ggccacgcct cggcgttgaa ccggatcaca tgcggcagca
    20581 gcacaccgtt gacgacgccg tgcggggcgt ccagcagccc gccgacctgg tggctcatgg
    20641 cgtgcgtggc gcccaggatg gcgttggtga acgccatgcc cgcgtgcagc gcggcctgcg
    20701 ccatcgccag gcgggcgccg aagtccctgg gctccagctg ggtgcgcagc aggttcccca
    20761 tgatcagctc gaccgcgtgc agggcgtgca tgtcggtgag cggattgtgc gcccgggaca
    20821 cgaacgcctc gatggcgtgg gtgagcgcgt ccagtccggt ggccgcgttc agccagggcg
    20881 gcatggtggt cagcagccgc gggtcgatca ccgagatgtc ggggacgagc gtccggctca
    20941 cgatggtgat cttgatgtgg gcggtggtgt cggtgatcac cgcgaactgg gagacgtccg
    21001 ccccggtgcc ggacgtggac ggcaccatca cggtcggcgg gatcggctgg acgacacggt
    21061 ccacaccttc gtagtcgagg atccgcccgc cgttgcccga caggatcgcc acacccttgg
    21121 ccgcgtcgat gaccgagccg ccgcccacgc ccacgatcac atcgcagccg ctctcgacgt
    21181 accgctcgta gccgccctgg acctcgtgat ccttgggatt gggcgtgatg tcggaccaca
    21241 ccacggggcg cagacccgcc cgcctcagat gggccaccgc ctcgtccgcc cagcccgccg
    21301 ccatcagccc ggggtcggtg accaggaagg gacgccggcc ccccagacgc agcgcgcaat
    21361 ggcccagctc ggtgagcgag cccgggccga agacgatctc ggggacatgg aacttggcca
    21421 gccgcgccga ctcccggacg gcggccgtgc tcgtgcgctg cgggcccagc agctcgccct
    21481 ggtgctcgtc gcggctccag tcggcgctcg ggtcgttccc gcccgtcact acctcctcgg
    21541 acgtgagcga catgcgacct cccaagggcg tgcagcccgc tacgcagacg caacgccgat
    21601 tttacgccgg ggtggctgac aacactggcg tccgaccggc aaccacgcta ggcgagccct
    21661 cccggacgcg acacctgtcc gatcggacag gtacccagga cccggccgga ggggtgcgcc
    21721 ggaccggcca aggtggacaa tagtcgcttg tccccgttct acggcaggga aaagcacatg
    21781 tcacaccgca gtgacctgcg gcgccgcagg gtcagcctgg agagcgagtg gtcccgctgg
    21841 gtgccgcgca tgaaagcggc gggatcgcat ccggtcggcg tggagacggt gcgccgcgat
    21901 gtcaccgagt cctggatgcg gtcactgagc agtgtggacc ccgaccggga cagcgccccc
    21961 gtcaccgacg agggacgcgt cgaggaccgc tggagcggat caccgctgcg cgcccccgtc
    22021 gacggcctcg ccgacgaact gcgcgacatc gccgacgacg ccggcttcgt caccgccgtc
    22081 accgacgagt ccggcaccat cctgtggacc tgcggcggac ctaccatgcg ccgccgcgcc
    22141 gaacgcgtca acttcgcccc cggcggccgg tgggacgaac acgccatggg caccaacgcc
    22201 ctctccctcg ccctgcgcac cggccgcccc acctccgtct tctccgccga acacctggtc
    22261 accgccctgc acggctgggt ctgctactgc gcccccgtgc acggacccga cggacgcatc
    22321 ctgggcgtgc tggacatgtc cagcacctgg gaccgctcca acccgctggc catggccacc
    22381 gtgcgctcgc tcgtcgccac catcgaatcc cgcctgcgca ccgaggcccc cgtcccaccc
    22441 ccacgcacca ccagccccct caacctcacc tgcctcggga gcgaacaggc actccgcgac
    22501 ggcacccccc tgcccctgcg cccccgccag gtcgagatcc tcaccctgct cgccctggaa
    22561 cccgacggct tctcacccga gcgcctgcgc gacgccctct acggcgaccg agccgtcacc
    22621 acctccacct tcaaggccga gatctcccac ttgcgccgcg ccctcgacgg cggcgtcgcc
    22681 acccgccgct acgccctcac caccccc
//
