LOCUS       NZ_CP104794            11416 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7791115
            Orig. end                         :: 7802531
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11416
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11416
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11416
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6416](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6416
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(261..276)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.57"
                     /tool="antismash"
     gene            complement(761..1984)
                     /locus_tag="N6J21_RS34205"
     CDS             complement(761..1984)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="N6J21_RS34205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_261720153.1"
                     /transl_table=11
                     /translation="MNPDAAPRARLRHLGGDGPRPRDFEEFYTQYFSSLIAQASRYADD
                     RETAADAVQDALIDVFRRWDSIDNPAAYAGTAVRRTIWSHQQIRQTAQLSALAPDSSAF
                     ALDASGRIIDYVTLVTCLKDLPDMQRTITALYYFEGWKISDIAARLQIAEGTARAHLVH
                     ARRRLRQLLAPRPSSTATTEPHTNPTPEAVQTTPAPAPTPTVQRAQAGNSDAFTDLYDE
                     HADTIYRYLYLRLGSRKSAEDLTARTFHRALHTINEFTGKHRDFGSWLVAIARRLVAGE
                     AGDSEFHLKATAEDLLAHAPRALTITPSGQHSRILQAMAELSPPQQECLTLRFLAGLSV
                     SETARAMGKREGAIRTLQHRALRALDRTLTAEADLWSVLHDVGAHCTKAAQPTTHAHQR
                     IRARAGVQ"
     misc_feature    complement(1175..1177)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(1985..1999)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.88"
                     /tool="antismash"
     gene            complement(2059..2814)
                     /locus_tag="N6J21_RS34210"
     CDS             complement(2059..2814)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS34210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261720154.1"
                     /transl_table=11
                     /translation="MSESTLLHRRPLSIKPRRVTGATIREALRVIERHFESEEREKLRT
                     QFRVPRTSPDEDDGFILASADTLDALLQDPAILDALPMGAPVTLTNLTAVASNADRMLH
                     LAVSADGVEITIASANEDWARARHAELRRIFTPSRRFWDLRSTAQLRDFFVLGMAVDAL
                     LAALLYVLGLPAHLDGTFLSRAAFAAALAVPPLVSWGIGRLCLRRCAVRISSAEQRSLW
                     RSLDTMERLTFCMLLIAVITLIAGLLDGN"
     misc_feature    complement(2982..2996)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.74"
                     /tool="antismash"
     gene            complement(3437..3772)
                     /locus_tag="N6J21_RS34215"
     CDS             complement(3437..3772)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /locus_tag="N6J21_RS34215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261720155.1"
                     /transl_table=11
                     /translation="MRHQKQNCAPHFAEIEVDFEPAAEGFVFQVARGLSVEFLSTEDVS
                     HFVAAAALGIEEQLTSPENGQRVVIAARVVLRHVRAHESDSHELAFKIAGYLAARRALE
                     RAGSHPA"
     gene            complement(3928..4710)
                     /locus_tag="N6J21_RS34220"
     CDS             complement(3928..4710)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS34220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261720156.1"
                     /transl_table=11
                     /translation="MIDGGCLVTWTNSSDQQRIRAQRFSPDGLEKGPEIAVNTTKAFHT
                     DATVTLLSGGEYVVAWTNGDPLGGGGLCYRVFSFEGTPKTEEVHPNVAGFGFLGRSTLT
                     GLDNGRLVAGHIESTLKSDIGVLQTTAVASIFDPLGDGEVIISASAGSSKHFHRTCPAL
                     TALPDGRFVLAWVEESADTVETVPKVVAQLCSDSELNIGPKVQVSSGTPDKIFHLTASS
                     VFGSATADKIFLAWTHMTADGKTSVRGAVLTAGPSGLS"
     gene            4744..4995
                     /locus_tag="N6J21_RS34225"
     CDS             4744..4995
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS34225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261720157.1"
                     /transl_table=11
                     /translation="MSNSTLSGPLPDPWLVEAVNVTVPMSVANDLDKMQGVMKEVMTQL
                     GCPTCHSGRDVRISIARDFVVDADGGVQQAVVPIRMTR"
     gene            5001..6416
                     /locus_tag="N6J21_RS34230"
     CDS             5001..6416
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="N6J21_RS34230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_261720158.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-29, bitscore: 102.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGVGLTYVPGLDRVLDSVGDLLDVVEIEPQTLWRVRSDGRIV
                     VDEEALGRIEHLPGARLLHGVGNPVGGTCPPDDRHTVLVGDLAERLRAPWVSEHLAFNR
                     VSDERGGFGTGFMLPPCQTAAGARAAVRSIRAMADGVNCPLAVEVGTNYLRPRTGELSD
                     GAFARQVVEGGGCGLLLDLHNVLANERNGRGSVDAFLAELPLDRVWEVHLAGGTEHRGY
                     WLDSHSGLPDHDLLALVERVLPRLPALRAVLFEVTPGAVPDLDVSAVRDLLVGVKEWWA
                     SSSPLPPLSPPDPMPLPAGHDEGHTIPAEWEHTLGSLTIGREPDTPLARELVADPAVPL
                     LRELVTEFRGSALAGVLRYTTRLLFLTLGPEGVSRLLDAYMRAHPPRLFASEEAFAFAD
                     HLAGELSAVPWLAQVLQLDLGLLRARLDGVPCTVALDTDPTALLTDLGAGRLPVAPQRG
                     RYRVRLVDDGVSA"
     gene            complement(6439..7395)
                     /locus_tag="N6J21_RS34235"
     CDS             complement(6439..7395)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325486.1"
                     /locus_tag="N6J21_RS34235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_261720159.1"
                     /transl_table=11
                     /translation="MSTGSYVVETTRTADVAAREQTELWSERVATYQPRMDFRYGRAEA
                     FRGELIRQRSAAYQVVTWYSDEIEYARTPGLIRRAPDPDYRLLISLSGGMRLRQNDEEV
                     CLDPGTAGLFTLGTPFELRHDASLHGVVMSIPAQEIESPLNTKAPLATALNLTKGLGRV
                     LDTTLRIVHEERDHLTAPQFDAVCDRVVELLRMLAAGDDRPTAPGHLTEVEAMVRRYVR
                     ANAADPALSGPAMARALGWSLRQIQLALQQVGTTPRDLIREERLRLLRDRLRCTDCAHL
                     TITDLSYASGFSSPSAMSTAFHRRFGVSPREVRQRGR"
     gene            complement(7519..8763)
                     /locus_tag="N6J21_RS34240"
     CDS             complement(7519..8763)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656079.1"
                     /locus_tag="N6J21_RS34240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_261720160.1"
                     /sec_met_domain="p450 (E-value: 2.7e-10, bitscore: 38.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGQPRPLVIDPTGRDIHGEAARIRERGPVTLVELPDGVPAWAVSS
                     PELLKRLLTDPRVSKDPRQHWPRWINGEIAPEWPLFTWVAVQNMFTAYGGEHKRLRSLV
                     SKAFTARRTAALRPRIEEITTGLLDRIAEAGQDGRVVDLREEFCYPLPIGVISELFGLP
                     EEQGAELRAVVDGVFRTSATPEEVTDNYARLYAALGELVALKRRSPGDDLTSALIAARD
                     DDGDRRLSEQELLDTLVLMVSAGHETTVNLIDNAIHLLLTHPDQLARVRDGRTGWEDVV
                     EEALRVEAPVASLPLRYAVEDLRMSEFGGPDGLVIAKGEAILAAYAAAGRHPDKYGADA
                     DRFDVTRVDKEHLAFGHGVHFCLGAPLGRLEANIALRALFGRFPHLRLAVSEGELEPVE
                     SFISNGHRRLPVLLS"
     misc_feature    complement(8743..8745)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9127..9342
                     /locus_tag="N6J21_RS34245"
     CDS             9127..9342
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS34245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261720161.1"
                     /transl_table=11
                     /translation="MVPPGSAAGWDMPLVRVLGRGSHVVVPPVHRVMGPGLCWQIPPTR
                     EREWTDPSRLRAALRTALLSEASRTA"
     misc_feature    10005..10019
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     misc_feature    10463..10476
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.51"
                     /tool="antismash"
     misc_feature    10943..10956
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
ORIGIN
        1 cgacgaagta catcgagtac ggcacggcgt ccagcctcac cggcccgtac acgatctccc
       61 gcaccggcga ctgggcgggc tggggcagct accgcgaggg cccggccctc gtccagctcg
      121 acaacggcgc ctggcgcctg ttcttcgacg cctacggcga ggcccgctac tactacagcg
      181 acagctacga caccttcacg acgtggtcgc ccccgacagc cctcccgtcg atctcgggca
      241 cggcccgcca cttcacggtg gtgaaagaga cggtgacggg cggcccgacg ctaccgcgcg
      301 acaccacccg ctccttccag tcggcgaact accccacccg ctactggcaa cagcagtcgg
      361 cgctgttgaa cctcccggtg gtgaccgcga cgagcacgac cacggacaag caggcgtcca
      421 ccttcaccgt caccaccggc ctggccgacc ccaccgcgat ctccttccgc gacgcatccg
      481 gcaactacct gcgccactac gacttccggg cccgcttcga cgcgaacaac ggcacgacca
      541 cgttcgcgag agacgcgacg ttcatccccc gcaccggcac gacaccaggc tccctgcgct
      601 tcgagtcgta caactacccc ggccactacc tgcgccacta cgcctaccaa ctccgcgtgg
      661 accgcacgga cggaacggac ctgttccgcc aggacagctc cttcacgccg gtcacggcgt
      721 ggggctgagc cgcacgctca cctcccgggg gggcgacggt ctattgcact cctgccctgg
      781 ctcttatgcg ttggtgtgcg tgagtggttg gctgtgccgc tttggtgcag tgagctccca
      841 cgtcgtgcag aacgctccag agatcggcct ccgcggtgag tgttcggtcg agtgcgcgca
      901 gggctctgtg ttgcagtgtt ctgatcgccc cctctctttt gcccattgcc cgtgctgtct
      961 cactcacgga cagaccggcg agaaaacgca gtgtgaggca ttcctgctgg ggcgggctca
     1021 gctcggccat ggcctggagg attcgactgt gctgcccgga aggagtgatg gtcagagccc
     1081 gcggggcgtg tgccaagaga tcctcggcgg tggccttgag gtggaactcg ctgtcgcctg
     1141 cttctccggc caccaggcgg cgagcaattg ccactaacca ggaaccgaaa tcccggtgtt
     1201 ttcccgtgaa ttcgttgatg gtgtgcaggg cgcggtgaaa agtccgcgcg gtgaggtctt
     1261 cggctgattt cctgctgccc aggcgaaggt agagatagcg atagatggta tcggcgtgct
     1321 catcgtagag gtcggtgaag gcgtccgagt ttccggcttg tgctcgttgc accgtcggtg
     1381 tgggcgcagg agcaggagtc gtttggacgg cttcgggggt ggggttcgtg tgcggttccg
     1441 tcgtcgcggt cgaggaaggc cgcggggcaa ggagctgtct caggcggcgc cgggcgtgga
     1501 cgaggtgggc tcgcgcagta ccttcggcga tttggagcct ggcggcaatg tcgctgatct
     1561 tccagccttc gaagtagtac agggcggtga ttgtgcgttg catgtccggc agatccttca
     1621 gacaggtgac cagcgtcacg tagtcgataa tcctgccgct ggcgtcgagg gcgaaggctg
     1681 acgagtccgg tgcgagggcg ctcaactgcg cggtttgacg gatctgttga tggctccaga
     1741 tagttcggcg tacggcagtt ccagcgtagg cagcgggatt gtcgatgctg tcccagcggc
     1801 ggaagacgtc gatcaatgca tcctggacag cgtctgccgc cgtttctcga tcatcggcgt
     1861 agcggctggc ctgggcgatc aggctactga agtactgcgt atagaactcc tcgaagtccc
     1921 gcgggcgagg cccgtctcca ccgaggtggc ggagacgggc cctgggggcg gcatcagggt
     1981 tcactgtccg atgtcctcct ggatggcggt cgagcgggtc gcggcatggt tggcgtgagg
     2041 gttgggcgcg acggcggtct aattgccgtc cagcaagccc gcgatgagcg tgatcacggc
     2101 aatcaggagc atgcagaaag tcagtcgctc catcgtgtcc agggaccgcc acaacgacct
     2161 ctgctcggcc gaggagatcc gcaccgcgca gcgccggagg caaagccggc caatacccca
     2221 ggacaccagc gggggcacgg caagggctgc ggcgaacgcg gctcgcgaga ggaaggtgcc
     2281 atcgaggtga gccggcaggc ccagcacata cagcagtgcc gcgaggagcg cgtccaccgc
     2341 catgcccagc acgaagaagt cccgcagttg ggcggtggag cggagatccc agaaccggcg
     2401 gctgggggtg aagatacgcc gcagttcggc gtggcgggcg cgtgcccagt cctcgttggc
     2461 gctggcgatg gtgatctcca caccgtcagc cgacaccgcc aggtgcagca tccgatcggc
     2521 gttgctcgca acggcggtca ggttggtcag ggtgaccgga gcccccatgg gtagggcatc
     2581 caggatcgca gggtcttgca gcagagcgtc gagcgtgtcg gcggacgcga ggatgaagcc
     2641 gtcgtcctcg tcggggctgg tacgtggcac gcgaaattgc gtgcgcagct tttcgcgctc
     2701 ctccgattcg aaatggcgct cgatgacacg gagtgcctcg cggatggtcg cgccggtgac
     2761 acggcgcggc ttaatgctca ggggccgccg atgcagaagc gtcgactctg acatgtacag
     2821 ggtctctctt cagttgactc atccatcaga tgttcgactc gcccgccctt ctcggccaga
     2881 gactcggacg cgtcggcatc agcagtatca acaccccgat caacattcaa atttagcgtg
     2941 aggatacaca taactgccat gagcatcggg cttattgatg cgattgtcag gagaatcgat
     3001 cctgcgtgcc cggagagggc tgcgtgcatc ataattggcc aatgcgtgac ggagtgcatt
     3061 tacatgccgc aggggccttc tgccctcaca agggaaatcg tgtggctcgg ggtgggagcc
     3121 ggtaggtgct cccaccccaa ggagtctgcc cccgagtctc tggccggagc gaaggcaggc
     3181 tctatcacct gatgcgagtc aacgcatcgc acgtcggacg cgtccgacgc acaccattga
     3241 agtgcgctac gggccgcaaa aagctgcgtc aacgcgccaa gtcttcagtt acgcagccgc
     3301 agccgcagcc gcagccgcag ccgcagccgc agccgcagcc gcagccgcag ccgatctgcc
     3361 ggctgtccct gttcactcgg ccattccctg ttgggaggcg cggcgcgtaa ggtactccgt
     3421 tcccgtgggc gttgactcaa gcggggtggg agcccgcacg ctccaaggct cgacgggcgg
     3481 ccagatatcc cgcgatcttg aaggccagtt cgtgggagtc cgactcgtgc gcgcggacat
     3541 ggcgcaacac cacgcgcgcg gcgatcacga cccgctgccc attctcgggt gacgtgagct
     3601 gctcctcgat gccgagggcg gcagcggcga cgaagtggga cacgtcctcg gtggacagga
     3661 attccaccga cagcccccgc gccacctgga agacgaaccc ctcggcagcg ggttcgaaat
     3721 ccacctcgat ctcggcgaaa tgcggtgcgc aattctgctt ctgatgccgc acccggacac
     3781 ccctgatggg aaacggcgga aaccgaaccg gccctgaggt cataggggaa cgctacccca
     3841 gggagtgatg ggggttcact accgcccggc gagttcttgg agggagtcca gtgacgggtg
     3901 atcacgaggt gaggcgcgat tcgactctca tgacagtcca ctgggcccgg cggtcaggac
     3961 ggcgccgcgg acggaggtct tgccgtcggc cgtcatatgg gtccaggcca ggaagatctt
     4021 gtccgcagtg gcgctgccga agacggagga cgcggtcagg tggaagattt tatccggggt
     4081 gccggagctg acttggacct tggggccgat gttcaactcg gagtcggagc acagctgtgc
     4141 caccaccttc ggcacagtct cgactgtgtc cgcgctctct tccacccacg ccagtacgaa
     4201 gcggccatcg ggcagtgctg tcagggcggg gcaggtgcgg tggaagtgct tagacgagcc
     4261 ggcggaggcg ctgatgatga cctcaccgtc ccccagagga tcaaagatgc tggccacggc
     4321 ggtggtttgg agaacaccga tgtcggactt gagcgtggac tcgatgtgcc cggcgaccag
     4381 ccgtccgttg tcgaggccgg tcagtgtgct ccgtcccagg aatccgaatc cggcgacatt
     4441 ggggtggacc tcttcggttt tgggcgtgcc ttcgaatgag aagacccgat agcacagtcc
     4501 gcccccaccc agcgggtcac cgttggtcca cgcgacgacg tattctccgc cactcagcag
     4561 cgtcacggtt gcgtcggtat ggaaggcctt tgtggtgttg accgcgatct caggcccctt
     4621 ctccagcccg tccgggctga accgctgggc acgaatgcgc tgttggtcgc tgctgttcgt
     4681 ccaggtcacg aggcagccgc cgtcgatcat cctggtgaca cgcacgaacg agggagagcc
     4741 agcatgtcga acagcacgct cagcggtccg ctgccggacc cgtggttggt ggaggccgtc
     4801 aatgtcacgg ttccgatgtc ggtggccaat gacctggaca agatgcaggg cgtgatgaag
     4861 gaggtgatga cgcagctggg ctgtccgacc tgtcactccg gcagggacgt gcgtatcagc
     4921 atcgcccgtg acttcgtggt cgatgcggac gggggcgtcc agcaggcggt cgtccccatc
     4981 cgaatgactc gctgaccgag atgacccgcc tgggagtcgg gctcacgtac gtgccggggc
     5041 tcgaccgcgt cctggactcg gtcggtgacc tccttgatgt ggtcgagatc gagccgcaga
     5101 ctctctggcg ggttcgcagc gacggccgga tcgtcgtcga cgaggaggcg ctcggccgga
     5161 tcgagcatct gcccggagca cgcctattgc acggggtcgg caacccggtc ggcggcacct
     5221 gcccgcccga cgaccggcac acggttctcg tcggcgatct ggccgagcgg ctccgagccc
     5281 cttgggtcag cgagcatctg gccttcaacc gggtgtcgga cgagcggggc gggttcggga
     5341 ccggcttcat gctgccgccc tgccaaaccg cggctggcgc acgtgcggcc gtccggtcga
     5401 tccgggccat ggcggacggg gtgaactgtc cgctggcggt ggaggtgggc accaactacc
     5461 tccggccgcg gaccggcgaa ctgtccgatg gcgcgttcgc gcgccaggtc gttgagggcg
     5521 gtggctgcgg gctgcttctc gatctgcaca acgtgctggc caacgagcgc aacggccggg
     5581 gcagcgtgga cgccttcctg gccgaacttc cgctggaccg tgtatgggag gtgcatctgg
     5641 ccgggggcac cgagcaccgc gggtactggc tggattctca ctccggcctg ccggaccacg
     5701 acctgctcgc gctcgtcgag cgtgtcctgc cgcggttgcc cgccctgcgc gccgttctgt
     5761 tcgaggtgac accaggagca gtgccggacc tcgatgtgag cgcggtacgc gacttgctcg
     5821 tgggcgtgaa ggagtggtgg gccagcagct ctccccttcc cccattgtcg cctccggatc
     5881 ccatgccgct cccggcgggc cacgacgaag gtcacacaat tcccgccgag tgggagcaca
     5941 ctctgggaag cctgacgatc ggccgcgaac ccgatacccc gctggcccgg gaactggtcg
     6001 ccgatcccgc cgtcccgctg ctgcgcgaac tcgtgaccga gttcaggggc agcgcgctgg
     6061 cgggagtgct ccgctacacc acgcggctgc tcttcctcac gctcggaccc gaaggggtgt
     6121 cccggctcct cgacgcctat atgcgtgccc acccgccacg actttttgcc tcggaggagg
     6181 ccttcgcctt cgcggaccat ctggccggag aactctcggc ggtgccgtgg ctggcgcagg
     6241 tcctccagct cgacctcggt ctgctgcgcg cccggctcga cggcgttccg tgcaccgtgg
     6301 ccctcgacac cgatccgacg gcgctgctga ccgatctggg cgccggccgc ctgccggtgg
     6361 ctccacagag gggcaggtac cgggtgaggc ttgtcgacga cggtgtatca gcctgacggg
     6421 ctgaatgacg tggccgcgct agcgtccccg ctgccgcacc tcccgcgggc tcaccccgaa
     6481 gcggcggtgg aaggccgtgc tcatcgcgct gggggaggag aagccggagg cgtaggacag
     6541 gtcggtgatg gtcaggtggg cgcagtccgt gcagcggagg cggtcgcgga ggaggcggag
     6601 gcgttcctcg cggatcaggt cccggggggt ggtgccgacc tgctggaggg ccagttggat
     6661 ctggcggagg gaccagccca gggcgcgggc catggccggg ccggagaggg cggggtcggc
     6721 ggcgttggcg cgtacgtagc ggcggaccat ggcctcgacc tcggtgaggt ggccgggggc
     6781 ggtggggcgg tcgtcgccgg cggcgagcat gcggaggagt tcgacgaccc ggtcgcagac
     6841 ggcgtcgaac tggggcgcgg tgaggtggtc gcgctcctcg tgcacgatgc ggagggtggt
     6901 gtccaggacc cggcccaggc ccttcgtcag gttcaatgcg gtcgccaagg gggccttggt
     6961 gttcaggggg ctctcgatct cctgggccgg gatcgacatc acgactccgt gcagggacgc
     7021 gtcgtggcgg agctcgaagg gcgtgcccag ggtgaacagg ccggccgtgc ccgggtccag
     7081 gcacacctcc tcgtcgttct ggcgcagccg catcccgccg gagagggaga tcaggaggcg
     7141 gtagtcgggg tccggggccc ggcggatcag gcccggagtc cgggcgtact cgatctcgtc
     7201 gctgtaccac gtcacgacct ggtaggcggc gctgcgctgg cggatcagct caccgcggaa
     7261 ggcctcggcg cggccgtagc ggaagtccat ccggggctgg tacgtcgcga cgcgctcgct
     7321 ccacagctcg gtctgctcgc gcgccgcgac atcggcggtc ctcgtcgtct ccacgacgta
     7381 gcttcccgtg gacaacgcgt ttcctccgct gcgatgtgat ccgaacggcg ctgatggcag
     7441 ccttcagcaa ctcccttgtg ggagagggct gttactcgcc gcacagtagg gggtcgcaat
     7501 cggtttccgg aactcgaatc aggacaacag caccggcaga cggcggtggc cgttgctgat
     7561 gaaggactcc accggctcca actccccctc ggacacggcc agtcgcaggt gcgggaagcg
     7621 accgaagagc gcccggagcg cgatgttcgc ctccagcctg cccagcgggg cgcccagaca
     7681 gaagtgcacc ccgtgaccga aggcgaggtg ctccttgtcg acgcgggtga catcgaagcg
     7741 gtcggcgtcc gcaccgtact tgtccgggtg gcgcccggcg gcggcgtacg cggcgaggat
     7801 cgcctcgccc ttggcgatca ccaggccgtc cgggccgccg aactcgctca tcctcaggtc
     7861 ctcgaccgcg taccgcagcg gcaggctggc cacgggcgcc tccacccgca gcgcctcctc
     7921 gaccacgtcc tcccagccgg tccggccgtc gcggacccgg gcgagctggt ccggatgggt
     7981 cagcaggagg tggatggcgt tgtcgatcag gttgacggtg gtctcgtgcc cggcgctcac
     8041 catcagcacc agggtgtcca gcaactcctg ctcgctcagc cgccggtcgc cgtcgtcgtc
     8101 gcgggccgcg atgagcgccg aggtcaggtc gtcgcccggc gaccgccgct tgagagcgac
     8161 gagttcgccg agcgccgcgt acaaccgggc gtagttgtcg gtgacttcct cgggggtcgc
     8221 ggaggtgcgg aacacgccgt ccacgacggc ccgcagctcc gcgccctgct cctcgggcag
     8281 accgaagagt tcgctgatca cgccgatcgg cagcgggtag cagaactcct cgcgcagatc
     8341 gacgactcgg ccgtcctgcc cggcctccgc gatccggtcg agcagaccgg tggtgatctc
     8401 ctcgatccgg ggccgcagcg ccgcggtgcg ccgggcggtg aaggccttgg agaccaggga
     8461 gcgaagccgt ttgtgctcgc ccccgtaggc ggtgaacatg ttctgcaccg ccacccaggt
     8521 gaacagtggc cactccgggg cgatttcgcc gttgatccag cgcggccagt gctggcgcgg
     8581 gtccttggag acacgggggt cggtcagcag gcgcttgagc agttcggggc tgctcacggc
     8641 ccacgccggg actccgtcgg ggagttcgac cagggtcacc gggccgcgct cgcgaatccg
     8701 ggcggcctcg ccgtggatgt cacggccggt cgggtcgatc actaaggggc ggggttgtcc
     8761 cacgcaagcc tcctcggcgc tacaggcata cgaccggcaa cggtatgccg acgtatcgca
     8821 ccgggggagg gcgcgcggcg ggtgctgcgc accggagcaa gttctctgcg tccggcggca
     8881 agtcgcgggt gtgttccgcg ttctacggtt cgaggtgtcg gatcggagag ccggtgaggg
     8941 tgatggcagt cgagaggacc tcgtggtgga tgcctccggg cagcatcgag gtgcggaccg
     9001 tggccgcggg cggttggtgg gacgccgtac gcgcgcccct ggacctcgga gtgcaggcgc
     9061 tgtggcatct cggtgacgat tccggagcag tgatccggga cggtttcggg ggggcgcctg
     9121 tactggatgg tcccccctgg ctcggcggcc ggctgggaca tgccactggt gcgcgtactg
     9181 ggccgcggca gccatgtcgt ggtcccgccg gtccaccggg tcatgggtcc tggcctgtgc
     9241 tggcagatcc cgccgacgcg ggagcgcgag tggaccgacc cgtcccgtct gcgcgccgcc
     9301 ctgcgcacgg cgctgctcag cgaggcgtcc cgtaccgcct gagaaccgca agagacttcc
     9361 gccccggccg cacctgaacc cggccggggc ggaactgtcc cgggggtggg ctcagtcgag
     9421 gtcgaaccgg tcgaggttca tgaccttgtc ccacgccttc acgaagtccc gtacgagctt
     9481 ctcctgcccg tcggcgcagg cgtacacctc ggcgacggcg cggagctggg agttcgcgcc
     9541 gaagaccagg tcgacggcgg tggccgtcca cttcacctgg cccgtggcgc ggtcccggcc
     9601 ctcgtagacg ttctcgtccg tcgtcgagac cttccactcc gtgcccatgt cgagcaggtt
     9661 cacgaagaag tcgttggtca gcgttccggg ccggtccgtg aacactccgt gccgggactg
     9721 cttgtggccg gtgtccaggg cgcgcatgcc gccgatcagc gccgtcatct cgggcgcggt
     9781 cagggtcagc aggttggcgc ggtccagcag cagggtctcc ggggacagct tctcgccgga
     9841 cttcaggaag ttgcggaacc cgtcggcctg cggctccagc acggagaacg actccacgtc
     9901 cgtctgttcc tgggaggcgt ccgtacggcc gggcgcgaac ggcaccgtga cggcgtgccc
     9961 ggcgtcctgc gcggccttct cgacggccgc gcatccgccc agcacgatca ggtcggccag
    10021 ggagaccttc ttgccgccgg tctgcccgga gttgaactcc tgctggatcc gctccagggc
    10081 ctggacggtg ccggtcacct cgggcgcgtt gttgacctcc cagtccttct gcggcgccag
    10141 ccggatccgg gcgccgttgg cgccgccgcg cttgtcggtg ccgcggaagc tcgccgccgc
    10201 ggcccaggcg gtggtgacca ggttcggcac ggacaggccc gagtcgagga tcttcgcctt
    10261 gagcgcggtg atgtcctcgt ccccgaccag ctcgtggtcg accggcggca cggggtcctg
    10321 ccacagctgc ggctcgggga cccacggacc gaggtagcgg gagacggggc ccatgtcgcg
    10381 gtggagcagc ttgtaccagg ccttggcgaa ggcgtccgcg agccggtccg ggttctcgtg
    10441 gaagctcttg gtgatcggac cgtagaccgg gtccagcttc agcgcgaggt ccgtcgtcag
    10501 catcatcggc gcgtgccgct tggacgggtc gtgggcgtcg ggcacgtcgt tctgggcggc
    10561 ggggtcggtg ggcttccact ggtgggcgcc ggccgggctg gtcgtcagct cccagtcgta
    10621 cttgtacagg ttgtccagga agccgttgtc ccaccggacg gggttgttcg tccacgcgcc
    10681 ttcgaggccg ctggtgagcg cgtcgccgcc gacgccgctg ccgcaggtgt tgcgccagcc
    10741 gagtccctgc tgctcgatcg gggcggcctc gggctccggg ccgatgcagc tggggtcgac
    10801 cgcgccgtga cacttgccga aggtgtgtcc gccgatgatc agggcggccg tctcctcgtc
    10861 gttcatcgcc atccggccga acgtctcgcg gatgtcgcgg gcggcggcca gcgggtccgg
    10921 attactgttg gggccctcgg gattgacgta gatcaggccc atctgaacag cgccgaaagg
    10981 accggtgagt tcccggtcgc cggtgtaccg ctcgtcaccg agccaggtgt cctcggggcc
    11041 ccagaaaatc tcctcgggct cccagatgtc ctcgcgtccg aagccgaacc cgaacgtctt
    11101 gaaacccatg gattccatgg cgcagtttcc ggcgaaaacc agaagatccg cccaggagat
    11161 cttcttcccg tacttctgct tgaccggcca cagcaaccgg cgcgccttgt ccaggctcgc
    11221 gttgtccggc cagctgttca gcggcgcgga acgctgagcg ccgctgccgc cgccaccccg
    11281 gccgtccgcg atccggtacg ttccagcggc gtgccagctc atccggatga aaagggggcc
    11341 gtaatggccg tagtcggccg gccaccactc ctgtgagctg gtcatcacct cgaagacgtc
    11401 ccgcttgagc gcctcg
//
