LOCUS       NZ_CP104794            11284 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6643094
            Orig. end                         :: 6654378
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11284
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11284
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11284
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6284](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6284)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            844..1575
                     /gene="hemQ"
                     /locus_tag="N6J21_RS29880"
     CDS             844..1575
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="N6J21_RS29880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_093724802.1"
                     /transl_table=11
                     /translation="MSDDAPTTEPGRIPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDVSGLRADADVMIWWHAETSDQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARMHVR
                     EEIPFFTGRRKSVSELVAGLA"
     gene            complement(1572..3146)
                     /locus_tag="N6J21_RS29885"
     CDS             complement(1572..3146)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (106-478). E-value: 4e-21.
                     Score: 67.7. Matches aSDomain:
                     nrpspksdomains_N6J21_RS29885_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034041.1"
                     /locus_tag="N6J21_RS29885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_261719718.1"
                     /sec_met_domain="PF00561 (E-value: 9.8e-24, bitscore: 83.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVPVLYSAVGSLLLAGVGAAPASGAEAAAEVRGVAVAVARAEAG
                     GITFGSCSAEGEMPGSMRCGTVSVPLDYADPYGKQIELTVSRVRATHKDPNNSKRRVPR
                     QGALVFNPGGPGASGMYFPLIGLMPEWKRIAAAYDLVGYAPRGVGRSAPLSCQDPRKFF
                     KGPAQAPTHPSESYKRERIAQAKAYARGCAKRSGSALRHYHSLNNARDLDVLRAALGEE
                     KLTFMGASYGTYYGALYATLFPSHVRRMVFDAAVNPDPEQIWYRNNLDQSAAFEARWAD
                     MREWIAKHHKVYGLGDTPAKVLRSYERASTRLARKPAGGKVGPGQLQNAFLQAGYYDDY
                     WPYRAYALSAYLKGDPQPLVDIAAPVTEAAAEAENSRAVYTAVECNDGPWPTDWRIWDR
                     DNTRLARVAPFETWDNVWMNLPCAYWPVPRQRPLDVRTGPGELPPTLILAAERDAASPY
                     QGALELHRRLWHSVLVTERDAGTHGIGGGPNACVNAHLDAYLLEGRLPVRRASCAPHPE
                     PRPETEG"
     aSDomain        complement(1713..2828)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS29885_Abhydrolase_1.1"
                     /evalue="4.00E-21"
                     /label="N6J21_RS29885_Abhydrolase_1.1"
                     /locus_tag="N6J21_RS29885"
                     /protein_end="478"
                     /protein_start="106"
                     /score="67.7"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGMYFPLIGLMPEWKRIAAAYDLVGYAPRGVGRS
                     APLSCQDPRKFFKGPAQAPTHPSESYKRERIAQAKAYARGCAKRSGSALRHYHSLNNAR
                     DLDVLRAALGEEKLTFMGASYGTYYGALYATLFPSHVRRMVFDAAVNPDPEQIWYRNNL
                     DQSAAFEARWADMREWIAKHHKVYGLGDTPAKVLRSYERASTRLARKPAGGKVGPGQLQ
                     NAFLQAGYYDDYWPYRAYALSAYLKGDPQPLVDIAAPVTEAAAEAENSRAVYTAVECND
                     GPWPTDWRIWDRDNTRLARVAPFETWDNVWMNLPCAYWPVPRQRPLDVRTGPGELPPTL
                     ILAAERDAASPYQGALELHRRLWHSVLVTERD"
     misc_feature    3132..3147
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.89"
                     /tool="antismash"
     gene            complement(3265..4035)
                     /locus_tag="N6J21_RS29890"
     CDS             complement(3265..4035)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125238.1"
                     /locus_tag="N6J21_RS29890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_261719719.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAVAGTACTRLCLAAVHSAAADTDTGREHDLTLYE
                     AAFLSGGPRRVADLTLVSMARQRRLLLARTGWATVVDPRGRDDIEQSVIGAIGPEGQSR
                     IEPVRDAAAGAEAVGTLAERLVRAGLAVPAGARTNIAAAVRQVQFAAAVVLALGATALL
                     LPGASDLPRHLVALWFGLPLTLTLSCLAIARAEIHPYSRWASPAGQRLLTALTRHRGAG
                     DRAYLTTVAVHGIRAIGEPDLRAAFEHREQAWRD"
     gene            complement(4140..4913)
                     /locus_tag="N6J21_RS29895"
     CDS             complement(4140..4913)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073954.1"
                     /locus_tag="N6J21_RS29895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_261719721.1"
                     /transl_table=11
                     /translation="MRPRPPAPGRGILSGTGLIITGLTATLAALIFPVWSYADRGGTGE
                     ALLNAETVATEYGPLSGLDRDFLTKVRLAGLWELPAGEQARLKGNTTAVQKAGEHLVEG
                     HTFLDERVRDVAAKLSLQLPNEPTEQQREWLRTLDASEGSAYDWQFANLLRLAHGKVFS
                     LVAEVRASTRNTLVRKLAEDAGTTVLDHIQVLEDTGYVDFAALAREMAAGRTPPLTHTP
                     APPGPVTDPSPALPVTPSQQPSYSLPPAASRPPES"
     misc_feature    5000..5015
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.45"
                     /tool="antismash"
     gene            complement(5001..6284)
                     /locus_tag="N6J21_RS29900"
     CDS             complement(5001..6284)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657153.1"
                     /locus_tag="N6J21_RS29900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_261719722.1"
                     /sec_met_domain="DUF692 (E-value: 5.1e-96, bitscore: 319.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIAEAVERMPGIDWVEVVAENVCPGHLPASLLR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLTALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTKDALKVLCENVRIAQDALPVPLAVENIAALFSWPGEEMTEGQFLY
                     DLADRTGVRLLIDVANLHTNHVNRGEDPAEALAELPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRVDPPGVLLERDEHFPEPAELERELAEIRAAVEKGGGPKAPA
                     AVSRPAPEPAVCDDARERLGLAQAALLSALVAGTPAPEGFDRARLKVQARALAGKRADV
                     VAKVAPELPVILGPRYRRDFLAYARRTPMTGGYRRDALDFAGQLLDADGGLENRARKEL
                     TRWWQERSGPAPLAPSRLARIRRSLTRR"
     gene            complement(6407..7120)
                     /locus_tag="N6J21_RS29905"
     CDS             complement(6407..7120)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981936.1"
                     /locus_tag="N6J21_RS29905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_176712076.1"
                     /transl_table=11
                     /translation="MSQESAPADSPFRSEPGDRDLAPQFVLPLVVRIERSAPPGRTDAL
                     ETAARAVLELLADERANGNGEWAEAVRDWQDARIRKVVRRARGAEWRRAEALPGITVTG
                     KSAEVRVFPPVPLDGWPKDLARLQVSGTDLDDPEPPAAADPSAPVLWLNPALGMSAGKA
                     MAQAGHGAQLAWWGLAGEQRAAWRDAGFPLAVRTAPEERWPALTTSGLPVVRDAGFTEI
                     APGSCTVVADHPALR"
     gene            complement(7166..7348)
                     /locus_tag="N6J21_RS29910"
     CDS             complement(7166..7348)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675663.1"
                     /locus_tag="N6J21_RS29910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_093724814.1"
                     /transl_table=11
                     /translation="MSDSDYCMIAETPRPPKADGPPYAECVQCREPTEYPESYKGITLC
                     PVCEWREAERTACSG"
     gene            complement(7367..8209)
                     /locus_tag="N6J21_RS29915"
     CDS             complement(7367..8209)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034067.1"
                     /locus_tag="N6J21_RS29915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_261719723.1"
                     /transl_table=11
                     /translation="MIARVRLVTATCALAAALAACGTPQAPRAARPATPTPTATPSGPP
                     TLAPGPEGLTPVFRSAARTPAKTVALTFDADMTADQGARAASGERFDNPELIAALRGLK
                     VPATVFMTGRWAEEYPAQARDIGRDPLFEVANHSYSHYAFTGDCYGLPRLPEARMREDV
                     ERAYTAFRRAGVPDPMPYFRFPGGCYDRTALKALTSAGVTAVQWDVVSGDAFATDADAV
                     ARQVLDQVRPGSVVVMHCTRSAAPATERAVRTIVPELRRQGYRFVKVSELIGAAGEHH"
     misc_feature    8227..8241
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.42"
                     /tool="antismash"
     gene            complement(8273..9652)
                     /locus_tag="N6J21_RS29920"
     CDS             complement(8273..9652)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657145.1"
                     /locus_tag="N6J21_RS29920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PhoX family protein"
                     /protein_id="WP_093724818.1"
                     /transl_table=11
                     /translation="MSEHGTQSRRQLFARTGALGVSIAFAGNLTELFAGTAAAQTMGHS
                     GYGPLLPDPNGLLDLPKGFRYRVLSREGDRLRSGEGKVPSNHDGMTALPGRHGRVHLVR
                     NHENRHNGRIGVPTVDGLTYDPAGKGGCTALTLDPHGEVLSERVGIAGTAVNCAGGPTP
                     WGTWLTCEETEDKAGTNGYTKDHGFIFEVDPADPHRSGAVPLTAMGRFQHEAIAVDPRT
                     GVVYETEDAFEKPFGLFYRFLPRHPRGGPGSLRAGGRLQAMRVPGVPDLSSIQETGTSF
                     DGIEWVDVPDPLAAETPIRFQDFGRRGITHAQKLEGCYWGGHSVYFVSSFARSAEGSAG
                     DHYGQIWRYDPGRRRLTLVIVFGPDTDVQLPGDSPDNICLAPSGGLMVCEDGEGAQHVY
                     GVTRRGEVYAVARNAQNIGTPEDPEWGEFAGVTFSPDGRTMYVNCYNPGTTFAVRGPWR
                     G"
     gene            complement(9743..10531)
                     /locus_tag="N6J21_RS29925"
     CDS             complement(9743..10531)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657144.1"
                     /locus_tag="N6J21_RS29925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="slipin family protein"
                     /protein_id="WP_093724820.1"
                     /transl_table=11
                     /translation="MLEDVLVAAVTAVSVSAVYLAAAARVVKQYERGVVLRLGRLRGEV
                     REPGFTMVVPVVDRLHKVNMQIVTMPVPAQEGITRDNVTVRVDAVVYFKVVDPASALIK
                     VEDYRFAVSQMAQTSLRSIIGKSELDDLLSNREKLNEGLELMIDSPAVGWGVQVDRVEI
                     KDVSLPEAMKRSMARQAEADRERRARVINADAELQASKKLAEAAQQMADTPAALQLRLL
                     QTVVAVAAEKNSTLVLPFPVELLRFLEKAQQPLPERPEGG"
     misc_feature    complement(10717..10732)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.28"
                     /tool="antismash"
ORIGIN
        1 ccaggccaag gccgccgcca acccgcagac cggccccgtg ttcatgggca tcgagggcgg
       61 catcggcacc ctcccgctcg cggtcgcgga cgcggtgcgc gccaagggcg gcgagatcct
      121 caccggcgcc cccgtgaccg cgctgcgccg cgggacggac ggctggaccg tcgtcaccgg
      181 cgaccggacc ctgcacgcgg acggcgtcgt cctcgccgta cccgccccga ccgccgccgg
      241 tctgctgcgc gccgatttcc ccgcggccgc cgccgaactg gccgccgtgg agtacgcctc
      301 catggccctg atcaccctcg cctaccgccg cgcggagacc gacctgcccg agggcagcgg
      361 cttcctggtg ccgcccgtcg acggccgcac catcaaggcc tccaccttcg cctcccgcaa
      421 atggggctgg atcgccgagg aggaccccga cctgctcgtc ctgcgcacct ccgtgggccg
      481 ccacggcgag accgagatcc tccagcgcga cgacgccgga ctcgtcgagg tctcccggca
      541 cgacctcaag gcggccaccg gcctcgacgc gaccccggtc gccacccgcg tcacccggtg
      601 gaacgacgga ctgccccagt accccgtcgg ccaccacgcg cgcgtggccc gcatccgcga
      661 gcacctggcc gcggcaccgg gcctggccgt gtgcggcgcg gcctacgacg gcgtgggcat
      721 ccccgcgtgc atcgcgagcg cgtatgccgc cgtcgaccag atccacggtg acctgcgcgc
      781 agtgcaggag ctcaccgcga acccggtgca gagtctgcac ggaggagcag gagaataagg
      841 gacatgagtg acgacgcccc caccaccgag cccggcagga tcccgaacaa gggcaagctg
      901 gccaaggacc tcaacgaggt catccgctac accctctggt ccgtcttcag gctgaaggac
      961 gtgctccccg aggaccgcgc cggatacgcc gacgaggtcc aggagctgtt cgaccagctc
     1021 gccgccaagg acgtgacgat ccgcggcacc tacgacgtgt ccgggctgcg cgccgacgcc
     1081 gacgtcatga tctggtggca cgccgagacc agcgaccagc tccaggaggc gtacaacctc
     1141 ttccgccgca cgaagctggg ccgcgcgctg gagccggtgt ggtcgaacat ggcgctgcac
     1201 cgccccgccg agttcaaccg ctcgcacatc ccggcgttcc tcgccgacga gacgccccgc
     1261 gactacgtca gcgtctaccc cttcgtgcgc tcctacgact ggtacctgct gccggacgag
     1321 gaccgccgcc gcatgctcgc cgaccacggc aagatggccc gcggctaccc ggacgtgcgc
     1381 gccaacaccg tcgcctcgtt ctcgctgggc gactacgagt ggatcctcgc cttcgaggcg
     1441 gacgaactgt accgcatcgt cgacctgatg cgtcacctgc gcggctcgga ggcgcggatg
     1501 cacgtccgcg aggagatccc cttcttcacg ggccgccgca agtccgtgag cgaactggtc
     1561 gcggggctcg cctaaccctc ggtctccggg cgcggctccg ggtgcggtgc gcaggacgcg
     1621 cgccgcaccg gcagccgccc ctccagcagg tacgcgtcca ggtgggcgtt gacgcaggcg
     1681 ttggggccgc cgccgatgcc gtgcgtgccc gcgtcccgct cggtcaccag gaccgagtgc
     1741 cacagccgcc ggtgcagctc cagcgcgccc tggtaggggg aggcggcgtc gcgttcggcg
     1801 gccaggatca gcgtcggcgg gagttcgccg ggtcccgtac ggacgtcgag cggccgctga
     1861 cgcggcaccg gccagtaggc gcagggcagg ttcatccaca cgttgtccca cgtctcgaac
     1921 ggggccacgc gggcgagccg ggtgttgtcc cggtcccaga tccgccagtc cgtgggccag
     1981 gggccgtcgt tgcactcgac ggccgtgtag accgcgcggc tgttctccgc ctcggccgcc
     2041 gcctccgtga ccggggccgc gatgtcgacg agcggctgcg ggtcgccctt cagataggcc
     2101 gacagcgcgt acgcccggta gggccagtag tcgtcgtagt agccggcctg gaggaaggcg
     2161 ttctgcagct ggccggggcc caccttgccg cccgcgggct tcctggcgag ccgggtgctc
     2221 gcccgctcgt agctgcgcag caccttcgcc ggggtgtcgc ccagtccgta gaccttgtgg
     2281 tgcttggcga tccactcccg catgtccgcc cagcgggcct cgaacgccgc ggactggtca
     2341 aggttgttgc ggtaccagat ctgctccggg tcggggttca ccgccgcgtc gaacaccatg
     2401 cggcgcacat gtgaggggaa gagggtcgcg tacagcgcgc cgtagtacgt gccgtacgag
     2461 gcgcccatga acgtcagctt ctcctcgccc agcgcggctc gcaggacgtc caggtcacgg
     2521 gcgttgttga gggagtggta gtggcgcagg gcgctgccgg accgcttcgc gcagccccgg
     2581 gcgtacgcct tggcctgcgc gatccgctcg cgcttgtagg actccgaggg gtgggtgggt
     2641 gcctgcgcgg gccccttgaa gaacttgcgc gggtcctggc aggacagcgg ggcgctgcgg
     2701 cccaccccgc gcggggcgta gccgaccagg tcgtaggcgg cggcgatgcg cttccactcg
     2761 ggcatcaggc cgatcagcgg gaagtacatg ccggaggcgc cggggccgcc ggggttgaac
     2821 accagcgcgc cctgccgggg cacccgccgc ttgctgttgt tcgggtcctt gtgggtggcc
     2881 ctgacccggc tgaccgtcag ctcgatctgc ttgccgtacg ggtcggcgta gtcgagcggg
     2941 acactgacgg tgccgcaccg catgctcccc ggcatctcgc cctcggcaga gcaggacccg
     3001 aatgtgatcc cgcccgcctc ggcccgggcg accgccacgg cgaccccccg cacctcggcc
     3061 gcggcctcgg cccccgaggc gggcgccgca ccgaccccgg ccaacagcag cgacccgacg
     3121 gccgagtaga gaacgggaac cctcatcacg tgtcccttcg gtgcgcgtac gcaacaaagg
     3181 ggatgtttcg tcccgggatt gcggaaggca agcaccaccg ggccggtgtc ggggtcaatg
     3241 ccccctgtgc gcccccgcgc ggggtcagtc gcgccaggcc tgctcgcggt gttcgaacgc
     3301 cgctctcagg tccggttcgc cgatggcccg gatgccatgg acggcgaccg tggtgaggta
     3361 ggcgcggtcg ccggcacccc ggtggcgggt cagggcggtg aggaggcgct ggccggcggg
     3421 cgaggcccag cgggagtagg ggtggatctc ggcgcgggct atggccaggc agctcagggt
     3481 gagggtgagc gggagaccga accagagggc gaccaggtgg cgggggaggt cggaggcgcc
     3541 cggcagcagg agggcggtgg cgcccagggc gaggacgacc gccgcggcga actgcacctg
     3601 gcggaccgcc gccgcgatgt tggtgcgggc tccggccggt accgcgaggc ccgcgcggac
     3661 caggcgctcg gcgagggtgc ccacggcctc ggcaccggcc gccgcgtccc gtaccggctc
     3721 tatgcgggac tggccctcgg ggccgatcgc gcctatgacg gactgttcga tgtcgtcccg
     3781 gccgcgcggg tcgacgaccg tcgcccagcc cgtgcgggcg agcagcagcc ggcgctggcg
     3841 ggccatggag accagggtca gatcggcgac ccggcggggg ccgccggaca ggaacgcggc
     3901 ctcgtagagc gtcagatcgt gttcccggcc cgtgtccgtg tccgcggccg ccgagtggac
     3961 ggcggccagg cacagccggg tacaggcggt gccggcgacg gcccaggcca ggagcaggag
     4021 aaggacccag aacatgccgc gtttctatgc caaaggcgca tgaggcgcca tgccttgttc
     4081 agtatccgga ccgagcgttg tcggtatgtg acgttccgtt cgaggttcgc gggggtctgt
     4141 cacgattccg gtgggcggct cgccgcgggc gggagcgagt aggagggctg ctgcgacggg
     4201 gtcaccggga gcgccgggga ggggtcggtc acgggcccgg gcggcgccgg ggtgtgggtg
     4261 agcgggggag tcctcccggc ggccatctcc ctggccaggg ccgcgaaatc gacgtacccg
     4321 gtgtcctcca ggacctggat gtggtccagc acggtggtgc ccgcgtcctc ggcgagtttg
     4381 cgcaccagcg tgttgcgggt gctcgcgcgg acctcggcga ccagcgagaa caccttgccg
     4441 tgcgccagcc gcagcaggtt ggcgaactgc cagtcgtacg cggagccctc actggcgtcc
     4501 agggttcgca gccactcccg ctgctgctcg gtgggctcgt tgggcaactg gagggagagc
     4561 ttggccgcga cgtcgcggac ccgctcgtcc aggaaggtgt gcccctcgac gagatgctcg
     4621 cccgccttct gcacggccgt ggtgttcccc ttcagccgcg cctgctcacc ggcgggcagc
     4681 tcccagagcc ccgcgagccg gaccttggtg aggaagtcgc ggtccaggcc cgacaggggg
     4741 ccgtactccg tggccacggt ctcggcgttg agcagcgcct caccggtccc gccgcggtcg
     4801 gcgtacgacc acaccgggaa gatcagtgcc gcgagggtcg ccgtgaggcc ggtgatgatg
     4861 agtccggttc cgctgaggat gccccggccg ggggcgggcg gtcgcggtcg catggtgcct
     4921 cctggtacgg caccgcacgt cagtgcgttt cgaggggtgg gacagaatcg gcatattaac
     4981 cactgatccc ggcccctcgt tcaccggcgg gtcagcgacc gccggatccg cgccagccgg
     5041 gacggcgcca gcggcgccgg gccggaacgc tcctgccacc accgcgtcag ctccttcctg
     5101 gcgcggttct ccagcccccc gtccgcgtcc agcagttgac cggcgaagtc cagcgcgtcg
     5161 cggcggtagc cgccggtcat gggggtgcgc cgggcatagg cgaggaaatc ccgccggtag
     5221 cgcgggccca ggatgacggg cagttcgggt gcgaccttcg ccaccacgtc cgcccgtttc
     5281 ccggccagcg cccgcgcctg caccttcagc cgcgcccggt cgaaaccctc gggtgcgggg
     5341 gtgcccgcga ccagggcgga gagcagtgcc gcctgggcga ggccgaggcg ctcgcgggcg
     5401 tcgtcgcaga cggcgggctc cggggccgga cgggacacgg cggccggtgc cttcggcccg
     5461 ccgcccttct cgacggccgc ccggatctcg gccagttccc gctccagctc ggccggctcg
     5521 gggaagtgct cgtcgcgttc caggagcacg ccgggcgggt ccacgcggga ggcgaggtcg
     5581 gtgaggatgt cgagcaccgg gcgcgggacc gggtgggcgt ggctgtcgtg ccagacgccg
     5641 tcccgctcga agccgcccgc gacatgcacg tacgcgagcg cctccagggg cagttcggcg
     5701 agcgcctcgg cggggtcctc gccccggttg acgtggttgg tgtgcaggtt ggcgacgtcg
     5761 atcagcagcc ggaccccggt gcggtcggcg aggtcgtaga ggaactggcc ctcggtcatc
     5821 tcctcgccgg gccaggagaa cagggcggcg atgttctcca cggcgagcgg tacggggagg
     5881 gcgtcctgag cgatccggac gttctcgcac agcaccttca gggcgtcctt ggtacgcggc
     5941 accggcagca ggtgaccggc ctccaggcgc ggggaggcgg tgagcgggcc gcccgcccgg
     6001 acgaacgcga tgtgctcggt gaccagcggc gagcccagcg cctcggcccg ctcggccagc
     6061 gccgtcagcc gtccctcgtc ggggcggtcg gcgccgccga ggccgagcga gacaccgtgc
     6121 ggcacgacgg tgaccccgcg ctcgcgcagc cgcagcagcg aggcgggcag atggccgggg
     6181 cacacgttct cggcgacgac ctcgacccag tcgatgcccg gcatccgctc cacggcctcg
     6241 gcgatctccg gccgccagcc gatccccgtc cccagtcgcg tcatcaccgt ccccctcccg
     6301 cgtgttccct tgcgtgcagg gggtatggcc ccggcccgcg gaggtgaatc caaccagagc
     6361 gtgcttcaga gcaacatttg aggttggacc gttccgcgcg ccccgctcag cgcagagcgg
     6421 gatggtcggc gacgaccgtg caggagccgg gggcgatctc ggtgaagccc gcgtcccgaa
     6481 ccacgggcag cccgctggtg gtcagcgccg gccagcgctc ctcgggcgcc gtccgcaccg
     6541 cgagcgggaa accggcgtcg cgccaggccg cgcgctgctc cccggccagc ccccaccagg
     6601 cgagctgggc gccgtgcccg gcctgggcca tggccttccc ggcggacatg ccgagcgcgg
     6661 ggttgagcca gagcacgggc gccgaggggt cggcggcggc cgggggctcg gggtcgtcga
     6721 ggtcggtgcc ggagacctgc agccgggcga ggtccttggg ccagccgtcc agcggcacgg
     6781 gcgggaagac ccgcacctcg gcggacttcc cggtcacggt gatcccgggc agggcctcgg
     6841 cccgccgcca ctcggcgccg cgggcccgcc gtacgacctt gcggatccgc gcgtcctgcc
     6901 agtcccgcac ggcctcggcc cactccccgt tcccgttcgc ccgctcgtcc gcgagcagtt
     6961 ccagcacggc acgggcggcg gtctccagcg cgtcggtacg cccgggcggc gcggaccgct
     7021 cgatgcgcac caccaacggc agcacgaact gcggcgcgag gtcgcggtca ccgggctcgg
     7081 aacggaaggg gctgtcggcg ggggcggatt cctggctcac ccgtcccagt ctgccagccg
     7141 gaagatcggt gccgcccggg gcgtctcagc ccgagcaggc cgtccgttcc gcctcgcgcc
     7201 actcgcacac cgggcacagg gtgatgccct tgtaggactc ggggtactcc gtgggctcgc
     7261 ggcactggac gcactcggcg tagggcgggc cgtccgcctt cggggggcgg ggggtctcgg
     7321 cgatcatgca gtagtcgctg tcgctcatgt gtccagcgta gaacgttcag tggtgctcgc
     7381 cggccgcgcc gatcagttcg gagaccttca cgaagcggta cccctgtcgg cgcagctcgg
     7441 ggacgatggt gcggacggcg cgttcggtgg cgggggcggc gctgcgggtg cagtgcatga
     7501 ccacgaccga gccggggcgg acctggtcca ggacctgccg ggccaccgcg tccgcgtccg
     7561 tggcgaaggc gtccccgctg accacgtccc actgcacggc ggtgacaccg gcggaggtca
     7621 gcgccttcag ggcggtgcgg tcgtagcagc cgccggggaa gcggaagtac ggcatcgggt
     7681 cgggcacccc ggccctgcgg aacgcggtgt acgcccgctc cacgtcctcg cgcatccgcg
     7741 cttccgggag cctcggcagg ccgtagcagt cgccggtgaa ggcgtagtgg ctgtacgagt
     7801 ggttggccac ctcgaagagc gggtcccggc cgatgtcccg cgcctgtgcc gggtactcct
     7861 cggcccaccg ccccgtcatg aacaccgtcg ccgggacctt cagcccgcgc agggccgcga
     7921 tcagctccgg gttgtcgaag cgctcgccgc tcgccgcccg cgccccctga tccgcggtca
     7981 tgtcggcgtc gaaggtgagg gcgaccgtct tcgccggggt gcgggcggcg ctgcggaaga
     8041 cgggggtcag gccctcgggg cccggggcca gggtcggggg cccggacggg gtggcggtgg
     8101 gggtgggggt ggcggggcgt gcggccctgg gtgcctgggg tgtcccgcag gcggccaggg
     8161 ccgcggccag ggcgcaggtc gcggtgacaa gtcgtacgcg tgcgatcacc gcacgaacat
     8221 aacgggatca atcagacaat cctcggattg ctttccgcgc cccggctgcg cgtcaccctc
     8281 gccaggggcc ccgcacggcg aacgtcgtcc ccgggttgta gcagttgacg tacatcgtgc
     8341 ggccgtccgg ggagaaggtg acccccgcga actcgcccca ctcggggtcc tcgggtgtgc
     8401 cgatgttctg cgcgttgcgg gccacggcgt agacctcgcc gcgccgcgtc acgccgtaga
     8461 cgtgctgggc gccctcgccg tcctcgcaca ccatcaggcc gccgctgggg gcgaggcaga
     8521 tgttgtcggg ggagtcgccg gggagctgga catcggtgtc ggggccgaac acgatcacca
     8581 gggtgagccg gcgccggccg gggtcgtagc gccagatctg gccgtagtgg tcgcccgccg
     8641 agccctccgc gctgcgggcg aaggaggaga cgaagtacac cgagtggccg ccccagtagc
     8701 agccctccag cttctgcgcg tgggtgatgc ccctgcggcc gaagtcctgg aagcggatgg
     8761 gggtttcggc ggccagtggg tccggtacgt cgacccactc gatgccgtcg aagctggtgc
     8821 cggtctcctg gatcgacgac aggtcgggca cgccgggcac ccgcatcgcc tggagccggc
     8881 cgcccgcgcg cagcgagccc gggccgccgc gcgggtggcg ggggaggaag cggtagaaga
     8941 ggccgaaggg tttctcgaag gcgtcctcgg tctcgtagac gacgcccgtt ctcgggtcga
     9001 ccgcgatcgc ctcgtgctgg aagcggccca tcgcggtcag cggtaccgcc ccgctgcggt
     9061 gcgggtcggc ggggtcgacc tcgaagatga agccgtggtc cttggtgtag ccgttggtcc
     9121 cggccttgtc ctcggtctcc tcgcaggtca gccaggtgcc ccaaggagtg ggtccgcccg
     9181 cgcagttgac ggccgtgccg gcgatgccga cccgttcgga gaggacctcc ccgtgcgggt
     9241 ccagggtcag cgccgtacag ccgcccttgc ccgcggggtc gtaggtcagt ccgtcgacgg
     9301 tcgggacgcc gattctgccg ttgtgccggt tctcgtggtt gcgcaccagg tggacgcggc
     9361 cgtggcggcc gggcagggcc gtcatgccgt cgtggttgga gggcaccttg ccctcgccgg
     9421 agcgcaaccg gtcgccctcg cgggacagga cgcggtagcg gaagcccttg ggaagatcga
     9481 gcaggccgtt cgggtcgggg aggagggggc cgtaacccga gtggcccatg gtctgcgcgg
     9541 cggcggtgcc cgcgaagagt tcggtgaggt tgccggcgaa ggcgatcgag acgcccaacg
     9601 cgccggtgcg ggcgaacagt tgacggcggc tctgggtgcc gtgctcggac atggggggaa
     9661 ccttcctgct ggtggacagg acagtgtcga gccgtgtcta ccatctggtt cccctccggt
     9721 gaaccacgcg cgtagagcgt gttcagcctc cttcggggcg ttcgggaagc ggttgctggg
     9781 ccttttccag gaatctcagc agctccaccg ggaacggcag taccagcgtg gagttcttct
     9841 cggcggcgac cgccaccact gtctggagca ggcgcaattg gagcgcggcg ggggtgtcgg
     9901 ccatctgctg ggcggcctcg gcgagcttct tcgacgcctg gagctcggcg tcggcgttga
     9961 tgacgcgggc ccggcgttcc cggtccgcct cggcctgccg ggccatggac cgcttcatgg
    10021 cctcgggcag ggagacgtcc ttgatctcca cccggtccac ctgcacgccc caccccacgg
    10081 ccgggctgtc gatcatcagc tccagaccct cgttgagctt ctcgcggttg gagagcaggt
    10141 cgtccagctc gctcttgccg atgatcgacc tgagcgaggt ctgcgccatc tgggagaccg
    10201 cgaaccggta gtcctccacc ttgatcagcg cgctcgccgg gtccacgacc ttgaagtaca
    10261 cgaccgcgtc cacccgcacg gtcacgttgt cccgcgtgat cccctcctgg gcggggaccg
    10321 gcatggtcac gatctgcatg ttcaccttgt gcagccggtc cacgaccgga acgaccatgg
    10381 tgaacccggg ctcccgcacc tcgccccgga gcctgccgag gcgcagcaca accccgcgct
    10441 cgtactgctt gaccacccgg gccgcggcgg cgaggtacac cgcgctcacc gagaccgccg
    10501 tgacggccgc caccaggacg tcttcgagca tgacgagcct cctgcccaga gtcccgcgag
    10561 atgcccttct tgtaacgata tgcccgggtg gccgtacggt gacccgtcgg ggctcagggc
    10621 gcctcggtga ggcccttcgc gtcccgggcc agcgcggtga ggcgggagat ggcgcggaag
    10681 tacttcttgc ggtacccgcc cttgagcatc tcctcgctga acagccggtc gaacggcagc
    10741 cccgaggcca gcaccggcac ctcacggtcg tagagccggt ccgccaggac cacgagccgc
    10801 agggccgtcg actggtccgg gaccgggcgc acgtccgtca ggcacaccgc cctgaccccg
    10861 tcggtcagcg cgccgtaccg gctcgggtgg accctggaca ggtgctccag caggtgcggg
    10921 aagtcgtcga gggaggcgcc ctcggtggcg tacgccgcct tcgtcacctg ctcgtcgctg
    10981 aacggcgccg gtgcctcggg cagcccccgg tgccggtagt cctcgccgtc gatgcgcagc
    11041 ggccggaagt gcgccgacag gccctggatc tcgcgcagga agtccgccgc cgcgaaccgg
    11101 ccctcgccga gcttgccggg cagggtgttg gaggtggcgg cgagcgcgac ccccgcgtcc
    11161 accagcttgc cgagcagggt ggagaccagg acggtgtcgc cggggtcgtc cagctcgaac
    11221 tcgtcgatgc acagcaggcg gtggccggag agggtcttca ccgtctgctg gaagcccagg
    11281 gcgc
//
