LOCUS       NZ_CP104794            18184 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4737673
            Orig. end                         :: 4755857
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..18184
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="16"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..18184
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18184
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:13184](-)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..13184)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     misc_feature    complement(183..198)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            complement(619..1107)
                     /locus_tag="N6J21_RS21185"
     CDS             complement(619..1107)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072417.1"
                     /locus_tag="N6J21_RS21185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261719077.1"
                     /transl_table=11
                     /translation="MFNLLMSPRTTRTVRGLLGNLVEAAATAVRSIAPTDTPADQPAPA
                     EAPDPTDVYTDDDMPDASAIEAAAAEYERATDQARRADRGKRAAKKVLDKLPADIYGAW
                     RVFRTPSSRQTPDLAEITRIFKAHNLGPVPMKPCAPSLKVERIETVPADADTLAGVAA"
     misc_feature    complement(1211..1224)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.68"
                     /tool="antismash"
     misc_feature    complement(1218..1234)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.29"
                     /tool="antismash"
     gene            1591..1785
                     /locus_tag="N6J21_RS21190"
     CDS             1591..1785
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS21190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_093722595.1"
                     /transl_table=11
                     /translation="MNSRSQLGVTSTVAGLCALIAVGAVMATTNLSGGVRIGVTAVLVL
                     ALAGVMAVVGAAVARSRRQ"
     gene            complement(1836..2228)
                     /locus_tag="N6J21_RS21195"
     CDS             complement(1836..2228)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889623.1"
                     /locus_tag="N6J21_RS21195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PIN domain-containing protein"
                     /protein_id="WP_261719078.1"
                     /transl_table=11
                     /translation="MSGTLVLDCEGLSKLVRRTPELTAWLAAAEAEDVRVITSSVTLVE
                     ARDPRTNPARFDYAVSRVNIIPPTEAIARHASKLLVAAGLHGHKYALDAIVAATAMASP
                     SPVTVLTSDPEDLLMLCGSGVRVVKI"
     gene            complement(2225..2506)
                     /locus_tag="N6J21_RS21200"
     CDS             complement(2225..2506)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928178.1"
                     /locus_tag="N6J21_RS21200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_093722593.1"
                     /transl_table=11
                     /translation="MVATTSDPVPGEGPIRPVSVSLHEGTIAALKARTGKRGMSAYVEA
                     LIQRQLERDRLRELIEDAEADHGPVDQAAVEAKRAILRGDAAGSTDSA"
     misc_feature    2506..2519
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.32"
                     /tool="antismash"
     misc_feature    2506..2522
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.92"
                     /tool="antismash"
     misc_feature    2506..2522
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.55"
                     /tool="antismash"
     misc_feature    2511..2524
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.21"
                     /tool="antismash"
     misc_feature    complement(2521..2534)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.2"
                     /tool="antismash"
     gene            complement(2564..3844)
                     /locus_tag="N6J21_RS21205"
     CDS             complement(2564..3844)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="N6J21_RS21205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_261719079.1"
                     /transl_table=11
                     /translation="MTTTAPPTPRRPRALLTAALPAPGAPRLLALSWLIKTVGSGLYLT
                     TSTLFFTRVVGLPVHQVATALTTAGIAALAGAVPLGKLADRYGPRRTYTALLAIQFATM
                     AALAFARTFPVFAGLILVFLLAEQGSTAARGALVATAGEGAERVRLRAYLRVVTNIGIT
                     IGAAASALVIQADSKTGYMVLLWCTALTYPAAAVPLRRLRAPKPEPELAAEKSPRRGSG
                     WQVLRDRRYAVITLICGVLSLQAQVLSFAVPLWVLEHTEAPRVLVSAMVTVNTVMVICL
                     QIRFSRGAEDNARAAAMCRAAGLALLASCALASFTAGLSGWQAVTLLLLFAAALTLGEL
                     WLSAGSFGLSFNLAPDHMHGQYQGFFSLGRGTATALAPALIATLCLGDHPRVGWLVLGT
                     LFAAAGVAAPWALRVRGAPGRTPGTHA"
     gene            complement(3841..4998)
                     /locus_tag="N6J21_RS21210"
     CDS             complement(3841..4998)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS21210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_261719080.1"
                     /sec_met_domain="ATP-grasp (E-value: 1.9e-06, bitscore:
                     26.3, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRQVLLLNSDKPEVLRALARRPDVTVRVMTRKVYADLYRDHETA
                     YVDSFADLTQVEQAAYELAAHGPVDHVIAATEKSVVAAGLVRALLGVPGPGVDQSLWAT
                     HKRAMKDRLRAAGLPVTDYAQAATLSDVPRAAERTGWPIMIKPVFGAASKCTYRVDSAA
                     EFTERHRTGEFTDLAEKRLPIQVERLVRFTHEYHCDGVVHEGEVAHAAVSRYYLPPLRI
                     TPNLNSGYVADQKDEFSQKVLSLHHQVVATLALPAGVTHLEIFRTEAGPVIGEVAVRPG
                     GMGISRMWWHAFGLDLWEEFARVSTGEPPSHPPRTPHPATIGRTRLPALPGLRERVLMM
                     PGVLEVLTPEECASPSHLEAYFEAPDERSAERVIADLHRLAVPPA"
     gene            complement(5001..5666)
                     /locus_tag="N6J21_RS21215"
     CDS             complement(5001..5666)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /locus_tag="N6J21_RS21215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metallophosphoesterase family protein"
                     /protein_id="WP_250748665.1"
                     /sec_met_domain="Metallophos_2 (E-value: 9.7e-13, bitscore:
                     47.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHAVLTDIHGDTARLRAVLERIRAQDVDEVVCLGDVFECRVGKR
                     DAPAYEFKGSPTEVFDADPQLARLLDGVRLVRGNQEERIRALVPEAHLPDWTRPLLDAP
                     SEHRTDFARYCHGHSLPWRELEPGRWCPAEADFPGRALVHGHHHRSALYRLRPGSPTRV
                     ENVPVRFGEPVPLAPDGRYVVNVGSVTRCAPDRGPSPAWAVIDEAAATVTYHHLQLTE"
     gene            complement(5672..6874)
                     /locus_tag="N6J21_RS21220"
     CDS             complement(5672..6874)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /locus_tag="N6J21_RS21220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_261719081.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.1e-07, bitscore:
                     30.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSGRQVPATPRLAVLAGTPQLVCAARSLGISTVFVHDASEPAPP
                     VAAEADTAIAADLADTEALHAALAPLHAEHPFGRVLSLTERGLLPTAALAERLGLPGNS
                     LHTVRLLQDKQRMRELLNARGIGPVHTAAPRDAADLAAFCRTTDGPVVLKPTAGTGSQA
                     VFKVADAQAAEEAWRAFEAAGGTQPLAEEYLDGPEISVESFSHDGKHTVLAVTDKLVGP
                     AFVETGHTQPSALPAPVLAEVTDLVHAFLDAVGLVEGPAHTEVKVTAKGPRVLESHNRI
                     GGDKIRELLRRAYDLDVVTLTAACPFGLLSAPTAPPVPRRGAAIRFLTPPPGKVEHITL
                     PDTTGTTAEIHLDVAVGDPVHRVRASRDRAGYVIADGPDAAEAARICEELASRVRIGTS
                     G"
     gene            complement(6871..7365)
                     /locus_tag="N6J21_RS21225"
     CDS             complement(6871..7365)
                     /GO_function="GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /locus_tag="N6J21_RS21225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="WP_261719082.1"
                     /sec_met_domain="GST_N_3 (E-value: 7.2e-07, bitscore: 28.3,
                     seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVLFQRETCPDCKPVRELLTRLQVSYININVPKPREERHELIRT
                     TGSKFIPALVDGATVIPGRLRENADIIAYLKERFGDPDELAARTAGDSDEVAARTAGDS
                     DEVAAKTAGDSDELAAKTAGDSDEVAATTAGDPDEVAAKTAGGRPESVALPGRAAAEPV
                     A"
     gene            complement(7365..8507)
                     /locus_tag="N6J21_RS21230"
     CDS             complement(7365..8507)
                     /NRPS_PKS="Domain: Aminotran_5 (6-324). E-value: 3.4e-40.
                     Score: 130.0. Matches aSDomain:
                     nrpspksdomains_N6J21_RS21230_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_1_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658833.1"
                     /locus_tag="N6J21_RS21230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_093722589.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.2e-26, bitscore:
                     89.7, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 0.00018, bitscore:
                     19.6, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 0.00011,
                     bitscore: 20.3, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRLATPGPTEVPQRLLLAGAREIIHHRSTEMEQLIHEISGALP
                     PLFGTDAPVYTIASSGTGAMEAAVANCFSPGDEVLVVSNGYFGERFQAICRSYGLTVHV
                     VQSDWGTSADPERVAVAYREHPRIKGAFVVYSETSTGALNDVEAIGRIFRDTDVVVVVD
                     AISGLLVHPLEMDAWGLDVVLAASHKGFMLPPGLAFVALSEKAWTAVEKANGPNYYWSF
                     ERLRHFYPMSSSSPAVSLLLALHESLKMLNEEGPESFRARHAVLGKASERALLKLGFST
                     FVQAPHRRSHVITSALAPEGIDTGRLLKTLSTSYGVTMTGGQAHLKGKLIRVGHVGAAD
                     ALDLCGIFGALEMALLDLGHTFTPGSGVGEIIRTFHEEGR"
     aSDomain        complement(7536..8489)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS21230_Aminotran_5.1"
                     /evalue="3.40E-40"
                     /label="N6J21_RS21230_Aminotran_5.1"
                     /locus_tag="N6J21_RS21230"
                     /protein_end="324"
                     /protein_start="6"
                     /score="130.0"
                     /tool="antismash"
                     /translation="TPGPTEVPQRLLLAGAREIIHHRSTEMEQLIHEISGALPPLFGTD
                     APVYTIASSGTGAMEAAVANCFSPGDEVLVVSNGYFGERFQAICRSYGLTVHVVQSDWG
                     TSADPERVAVAYREHPRIKGAFVVYSETSTGALNDVEAIGRIFRDTDVVVVVDAISGLL
                     VHPLEMDAWGLDVVLAASHKGFMLPPGLAFVALSEKAWTAVEKANGPNYYWSFERLRHF
                     YPMSSSSPAVSLLLALHESLKMLNEEGPESFRARHAVLGKASERALLKLGFSTFVQAPH
                     RRSHVITSALAPEGIDTGRLLKTLSTSYGVTMTGGQA"
     gene            complement(8504..8968)
                     /locus_tag="N6J21_RS21235"
     CDS             complement(8504..8968)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658832.1"
                     /locus_tag="N6J21_RS21235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YjbQ family protein"
                     /protein_id="WP_093722588.1"
                     /transl_table=11
                     /translation="MIELTVESTGLNSHYDLTDRIAAALQEHGTGDGLAGVFAHGSTIG
                     LTVMRYEPGAVQDLLRALERIAPESPADGGRYLHELTTADPNGFSHLKSSLLGTSLLVP
                     FRDGRLAMSPSHRVVLFDFDLKPATRRVFLDAPRPATPAHSPDEIKENSR"
     gene            complement(8965..10086)
                     /locus_tag="N6J21_RS21240"
     CDS             complement(8965..10086)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS21240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="ATP-binding protein"
                     /protein_id="WP_261719083.1"
                     /transl_table=11
                     /translation="MTATTEYAALRLDFHGTHLDLEVAPGADIDGALAFLATHLVAEEA
                     KPGESDRDPVATLRVHAPDPERLAPPAGTATEDIYLRKSASEFFTVPARRAAVDGREYL
                     ECVKTGSRFRFDRAARVIDVTLGAGGHMDLVELVRDLVLRHQENTGAAVLHATAAYRDG
                     EVVLVTGAKGAGKSTVLMELVEHFGYQVLSGDKTVLHELPDGSVVAAGWPDYPHLGYGT
                     IAKYPGLKEIAGIGDDYTPAEGHAFSPLGKFAVDPLPFRDRFPSAPVGVRVPVAALLHP
                     AIGPGERTAVEPLTGSREEHAEVLRANVESAFDGANAGWHHYLDDHRAAHAAQRDRITG
                     ILSGVPAWTVTGPGDLTTENVPPAIAGPAGVAR"
     gene            complement(10083..11018)
                     /locus_tag="N6J21_RS21245"
     CDS             complement(10083..11018)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658830.1"
                     /locus_tag="N6J21_RS21245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_250748673.1"
                     /sec_met_domain="phosphonates (E-value: 5.9e-107, bitscore:
                     355.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates-like (E-value: 2.2e-130,
                     bitscore: 432.4, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 5.3e-62, bitscore:
                     208.2, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGSRPPQQEPNDPTAGAARRLRAALAAPRLARAMGAHSPLSARL
                     AEEAGFDVVWSSGLEISAAAGVPDANILAMGECLDAAADLAAAVDIPVLADCDSGFGNV
                     NNVIHMVRSYEARGVAGVCIEDKQFPKLNSFVEGNQDLAPLDDFAAKIRAAAETRTDMV
                     VVARLEALISGQGMAEALRRAEVYERAGADALLIHSKRKDPEEVFAFRESYRGDLPVIV
                     VPTTYNQVTVEELEQRGFAMAIYANQALRSSIRAMRETLTRIMRDGTTLNVESELAPLK
                     EVFDLQRMPQMLEQQERYESVGRELAEAAQ"
     gene            complement(11015..12061)
                     /locus_tag="N6J21_RS21250"
     CDS             complement(11015..12061)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271: 2-isopropylmalate synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /locus_tag="N6J21_RS21250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261719084.1"
                     /sec_met_domain="HMGL-like (E-value: 9e-38, bitscore:
                     129.1, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPTLLDCTLRDGGNQNDWQFTATDVRTIVATLDAARVDVIEVGY
                     RGGSGSKASATAGPSAHCTPEYLAALPDVSHAELAVMVVPTVCPVRAMDDLPDSPVSMV
                     RVAAYPWNIDGVPEYVRAVRALGLKVGVNLMAVSYTTEEQLAEIAGIVAAEAPDVFYIA
                     DSFGALTPDDVRGCVELLAERLPTPLGIHAHNNLGLAAANALAGLDAGVTWLDASLCAM
                     ARGAGNLATEQAAAFLTAWPKYATHADLPLVCEAAEYVAEQVLPRPMTVRRAEIAAGIN
                     DHHFYFQDRIDKISARHGLDPWQVGRRVGAARPRKVLDETVEDVCRELAGATTGRTEET
                     ERTDGGDA"
     gene            complement(12058..12657)
                     /locus_tag="N6J21_RS21255"
     CDS             complement(12058..12657)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /locus_tag="N6J21_RS21255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="WP_261719085.1"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.6e-17, bitscore:
                     61.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDIRDALGALLEREPAALTVSTCGYITRDVYNIRDRANHFYLVG
                     SMGMAAPIGLGIAVAHPDRRVLVLDGDGSFAMNPGCLPMIAEHRPNLVHVVLDNGAHES
                     TGGQRTAALGDPAAMALAAGYAAAHTVDDLDGLAALDLTGTPTLVRIRCELRGHPAGKR
                     VEQTPQELVERFRTHLAEGADAPSAGPLTSHLEGAV"
     gene            complement(12660..13184)
                     /locus_tag="N6J21_RS21260"
     CDS             complement(12660..13184)
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658827.1"
                     /locus_tag="N6J21_RS21260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_093722584.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 2e-08, bitscore:
                     32.8, seeds: 75, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPKSSLAAGPGTEVAEILLNAFVTRGHSHFTGVPCSLLKGFFRL
                     LEDPGCPAAYIPAPREDSALGVASGLAVAGARPVVLMQNSGLGYSLNVLTSFNLIYDVH
                     LPLVVSWRGHGGNDAVEHDVIGRELTRLLDVFGLPWTVLDNAHPEDSVEAFLKQYDDGR
                     STAVLIVREGI"
     misc_feature    complement(13050..13052)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    13710..13724
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            13940..15382
                     /locus_tag="N6J21_RS21265"
     CDS             13940..15382
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658826.1"
                     /locus_tag="N6J21_RS21265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_093722583.1"
                     /transl_table=11
                     /translation="MRAAEQDSGVGLVDDGAQAAVEAAEEIIEEASGGDGGVFAPAWSD
                     DRVAHLLERLTSDFVLDNTYREQHLSLTASENYPSKLVRMLGSALQGGFYEFAPPYPTE
                     AGEWAFPDSGANASLVGKLTGIGRQLFEAATFDWRPNGGSVAEQAVLLGTCGRGDGFVH
                     FAHKDGGHFALESLAAAAGVNTYHLPMVDRTLLIDVDRLATLCAEHPEIKLVILDQSFK
                     LRWQPLAQIRAALPEGVFLAYDASHDGALIAGGALPQPTLLGADAVHGNTHKTIAGPQK
                     AYIAFRDADHPRLRAVSDWVCPQMQSNSHAELIAPMYVALSEIALYGHDYARQILANAK
                     ALAHALHEEGVRVSGESFGFTETHQVHLVTGSAADALRLALGDLAHAGIRTTNIEVPGA
                     NGLHGLRLGVQAMTRRGLRETEMREVARLVAKVVLRRAEPTTVRAEVADLLQSHPLDRL
                     AFSFDSHVESPAAARLLGEVFR"
     gene            15460..17244
                     /locus_tag="N6J21_RS21270"
     CDS             15460..17244
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /locus_tag="N6J21_RS21270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="WP_261719086.1"
                     /transl_table=11
                     /translation="MLSAAVRPRRNDVPHAVISAEVWHTEWARDLVERLALRTVIADER
                     LSCERVLDTLAAQSVAFVCEELPVSGLAAMTWAARRTEALRRDDPLLGVVFMVEDPRAL
                     DPRALGPLVRTLSPGDTDLPGFLAEVQRVRREFHWEARNRRPASRPAGEAAARPARWAG
                     TVPNFNVKHPSMLLPYALLSARTRREVYCEVSPQEALLFVADSVGRGPAAAVAEVFRIL
                     RQDVDRLNSALGSRLRLHLDHADDYEVVQAACEAGFDSVMVDGSGRTLHQNVLFTNAAT
                     ELARRYGVLVEGEIGSIDGAGLRKWNKTTPGDFTRFAEDTDLDLIGAHVGQFHSFDYGF
                     AATRRSLAEVGLLDDRATGDDLHAFTEACASVEKELNDAGLPGYSAEHRLLAALARRAT
                     TEGDGPRLDAVFQEMRARVPVYQRPLLDALEKEWLERRLAKARRKTELWDEVFATEPAR
                     EHRYAAIDHGLVQTLTKVLDGTGRALVVHGGTSLSRVDLASLPAHGVARVNFGSGVYAD
                     YLEALRAHLPVDVNAATGEGTWKHLRRMSYLAEATRDWRSWTDAPPSFVDTFVSRLERC
                     YAAPLRGRTPEQAQGGRS"
     gene            17241..17738
                     /locus_tag="N6J21_RS21275"
     CDS             17241..17738
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658824.1"
                     /locus_tag="N6J21_RS21275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YbaK/EbsC family protein"
                     /protein_id="WP_093722581.1"
                     /transl_table=11
                     /translation="MSPFEQIVDLLDREKARYRVVEHPAEGRSEEVASVRGTTVSQGAK
                     ALVCRVKGIEAPAVLAVLAGDRRADLKKVVAAVEGKKGGFAPSELAEQLTGCAVGTIPP
                     LSLTGTLPVVVDEEFLAAHEEIAFNAGRLDRSVVMATEDYVRIVAPAVASIAAPVVPAA
                     AQ"
ORIGIN
        1 gcatccgagg tcggtcgccc gtcttctcca gagccgccgc cagcgcgtcc gacgccgtct
       61 cgaacgcggc caacgcggtc cgctcgtcct cggtctcggt ggccggcgcc gcgggcgggt
      121 cgaggaggat cgccatgcgg cgctgctcga cgcgcagggc gatcaggtgc gcggtgaggt
      181 cgcggaccag ggcgtcaacc tggtcgatgt ccaggtctgg ccaggtgccg tcggcctgga
      241 aggacagccg gggttcggcg ccggtccact ggacgagttc gaacgcgaga acttcgcggt
      301 ctttgtcgct gaggtagggg ccggtgaggg cgtggggggt gccgacgtgg cggtgttcgt
      361 gggggtcggc gtggctgtcg gggtcgccgg tgcaccagtc gatgccgtcg gggcaggcgg
      421 gggtgcggcc ggtgaggcgg gcgatggcgc cctcggcgat ctgcgcggcc agctccggcg
      481 tgtactcccc ggggagggtc ttgcgcatgg cctcgtcgac cgcggccggg aggaccgtgg
      541 cgagccagtc ggtggccggg ggctcggcgg gctggggctg ggtggggatg gcgggcaggt
      601 agttgtcggt ggcgttcatc aggcagccac ccccgccagg gtgtcggcgt cggccggaac
      661 ggtctcgatg cgctcgacct tgagggacgg ggcgcagggc ttcatgggga cggggccgag
      721 gttgtgggcc ttgaagatgc gggtgatctc ggcgaggtcg ggggtctgcc gggagctggg
      781 ggtgcggaag acgcgccagg cgccgtagat gtcggcgggc agcttgtcga gcaccttctt
      841 cgcggcgcgc ttgccgcggt cggcgcggcg ggcctggtcg gtggcgcgct cgtactcggc
      901 ggcggcggcc tcgatggcgg aggcgtcggg catgtcgtcg tcggtgtaga cgtcggtggg
      961 gtcgggggcc tcggccgggg ccggctggtc ggccggggtg tcggtggggg cgatggagcg
     1021 gacggcggtg gcggcggcct cgacgaggtt gccgagcagg ccacgcaccg tgcgggtggt
     1081 gcgggggctc atgagaaggt tgaacacagc ggatctcctg ttgcgtcagg ttgtgtccgt
     1141 caggccctcg gctggtgttg gcgcaccgcc gggggccgct ccgtctctcg ggcggagtag
     1201 gtacagttta gcaacgcatg ttccttgata gcaacgtatc cggcgatacg cgcccactgc
     1261 aatgcaggcg accccgcagg cgcaggccgt cggccctgta cgtatgccgt acacgtgagg
     1321 acgtgatacg ccgtgataac ccgagatacc aaccgacgga atacgcgcag gtcaggcggt
     1381 gtgtgaggct acggcgcagg tcgcgggagt gcccgaatag acctcgtaat gcgtaggtct
     1441 cgggttcgaa tcccgaaggc ggctccattc gaaagcccag ctcagagtca ctctgagctg
     1501 ggctttttcg ttcagcggtt acggcggcgg cgccggggcg tgtgctgcgc ggctgtccgt
     1561 ggtctcagcg gtggatgagg agcagcaggc atgaacagta ggagccagtt gggcgtcacc
     1621 tcgacggtgg ccggcctctg tgcgctgatc gcagtgggag cggtgatggc cacgacgaac
     1681 ctgtccggag gggtacgcat cggcgtcacc gccgtgctcg ttctcgcgct ggcgggcgtc
     1741 atggccgttg ttggagccgc tgtggctcga agccgtcgtc agtgaggctc ccgtgacagg
     1801 gtgctgtacc tcccggctgg cggaatcgcc gtgcgtcaga tcttgactac gcggacgccg
     1861 gagccgcaca gcatcaagag gtcctcgggg tccgaggtga ggaccgtcac gggagagggg
     1921 gaggccatcg cggtggcggc cacgatcgcg tcgagggcgt acttgtggcc gtggagtccg
     1981 gctgcgacga ggagcttgct ggcgtgacgg gcgatggctt cggtgggcgg gatgatgttg
     2041 acgcgggaga cggcgtagtc gaagcgggcc gggttggtcc tggggtcgcg ggcttcgacg
     2101 agggtgacgg agctggtgat gacccgtacg tcttcggctt cggcggcggc cagccatgcg
     2161 gtgagctcgg gggtgcgtcg tacgagtttg gacaggccct cgcagtcgag gacgagggtg
     2221 ccgctcacgc ggaatccgtc gagccggctg cgtcaccgcg caggatcgcc cgcttggcct
     2281 cgactgcggc ctggtcgacg gggccgtggt cggcttcggc atcctcgatc agttcgcgca
     2341 gccggtcgcg ctccaactgt cgctggatga gggcttcgac ataggcggac atgccccgct
     2401 tgcccgtacg cgccttgagg gccgcgatgg tgccttcgtg gagggagacg gagaccgggc
     2461 gaatcggacc ttcgccggga acggggtctg aggtggtggc gaccatggaa atatgttaac
     2521 aaaacttttg ttattcggtg tggtgattcg tcagggtgat tcgtcatgca tgggtgccgg
     2581 gcgtccgccc cggcgccccc ctcacccgca acgcccacgg cgccgccacc cccgccgctg
     2641 cgaacagggt gccgagcacc agccacccca cgcgtggatg gtcgcccagg cagagggtgg
     2701 cgatcagggc cggggccaga gctgttgccg tgccgcggcc cagggagaag aagccctggt
     2761 actggccgtg catgtggtcg ggggcgaggt tgaagctgag gccgaaggag cctgcggaga
     2821 gccagagttc gcccagggtc agggccgccg cgaagagcag gaggagggtg acggcctgcc
     2881 agccggacag gccggcggtg aatgacgcca gcgcgcagga cgcgaggagg gcgaggcctg
     2941 cggctcggca catcgccgcc gcccgggcgt tgtcctccgc tcctctgctg aagcggatct
     3001 ggaggcagat caccatcact gtgttgaccg tgaccatggc cgagaccagt actcgtggtg
     3061 cttccgtgtg ttccagtacc cacaggggga ccgcgaagga cagcacctgg gcctggagtg
     3121 acaggactcc gcagatcagt gtgatcacgg cgtagcggcg gtcccgcagg acctgccagc
     3181 ccgagccgcg gcggggcgac ttctcggccg ccagttcagg ctcaggcttt ggggcgcgca
     3241 ggcggcgcaa cggtaccgcc gccgccgggt acgtcagtgc cgtgcaccac agcaggacca
     3301 tgtagcccgt cttcgagtcc gcctggatca ccagggccga ggccgccgcg ccgatcgtga
     3361 tgccgatgtt ggtcaccacg cggaggtagg cccgcaggcg gacgcgttcg gcgccctctc
     3421 ctgccgtcgc caccaacgcg cctcgcgccg ccgtgctgcc ctgttccgcc aggaggaaga
     3481 ccaggatcag gcccgcgaac accgggaacg tgcgggcgaa ggccagggcc gccatcgtgg
     3541 cgaactggat ggcgaggagg gcggtgtacg tccgtctcgg gccgtagcgg tccgccagtt
     3601 tgcccagggg gaccgctcct gccagtgccg ctattccggc ggtcgtgagg gccgtggcca
     3661 cctggtggac cggcaggccg acgacccttg tgaagaacag ggtgctcgtc gtcaggtaca
     3721 ggccgctgcc caccgttttg atcagccagg acagggccag gaggcgaggg gcgcccgggg
     3781 ccgggagcgc cgccgtgagc agggctcggg ggcggcgggg ggtggggggt gccgtggtcg
     3841 tcatgccggt ggcaccgcca gtctgtgcag gtccgctatg actcgctccg cggacctctc
     3901 gtccggagcc tcgaagtacg cctccaggtg ggacgggctc gcgcactcct ccggggtcag
     3961 gacctccaga actccgggca tcatcagcac tcgttcgcgc aggccgggga gggccgggag
     4021 gcgggtgcgg ccgatggtcg cggggtgcgg ggtgcggggt gggtggctgg ggggttcgcc
     4081 ggtcgagacg cgggcgaact cctcccacag gtccaggccg aacgcgtgcc accacatgcg
     4141 ggagatgccc atgccgcccg gccgtaccgc cacttcgccg atcaccgggc ccgcctcggt
     4201 gcggaagatc tccaggtggg tgaccccggc cggcagggcc agcgtcgcca ccacttggtg
     4261 gtggagagac aggaccttct gtgagaactc gtccttctgg tccgccacat agccgctgtt
     4321 caggttcggt gtgatgcgca ggggcgggag gtagtagcgg gagaccgccg cgtgggccac
     4381 ctcgccctcg tgcacgactc cgtcgcagtg gtactcgtgt gtgaagcgca ccaggcgttc
     4441 gacctggatc ggcagccgtt tctccgcgag gtcggtgaac tcgccggtgc ggtggcgttc
     4501 ggtgaactcg gccgcggagt ccacgcggta cgtgcacttg gacgcagcgc cgaacaccgg
     4561 cttgatcatg atcggccaac cggtccgttc cgccgccctg ggcacgtcgg agagcgtggc
     4621 ggcctgggcg tagtccgtca ccgggagtcc cgccgcccgc agtctgtcct tcatcgcccg
     4681 cttgtgggtc gcccacagcg actggtcgac gccggggccg ggcacgccga gcagtgcgcg
     4741 gaccaggccg gccgccacca cgctcttctc cgtcgccgcg atgacatggt cgacggggcc
     4801 gtgggcggcc agttcgtacg ccgcctgctc gacctgggtc aggtcggcga agctgtcgac
     4861 gtacgccgtc tcgtggtcgc ggtacaggtc ggcgtagacc ttgcgggtca tgacgcggac
     4921 ggtcacgtcg gggcggcggg ccagggccct gaggacctcg ggcttgtccg agttgagcag
     4981 gagtacctgc cgggtcactg ctactccgta agttgaaggt ggtggtaggt gacggtggcc
     5041 gccgcctcgt cgatcacggc ccaggcgggg gaggggccgc ggtccggggc gcagcgggtc
     5101 acggagccca cgttgacgac gtagcggccg tcgggggcga gcgggaccgg ctccccgaaa
     5161 cgtacgggca cgttctccac gcgcgtcggg ctgcccggcc gcaggcggta cagggcactg
     5221 cggtggtggt ggccgtgcac cagtgcccgg ccggggaagt ccgcctcggc gggacaccac
     5281 cgccccggct ccagttcccg ccagggcaag gagtggccgt ggcagtaccg ggcgaagtcc
     5341 gtgcggtgct ccgagggcgc gtccagcagc gggcgcgtcc agtcggggag gtgggcctcg
     5401 ggcaccagtg cgcggatccg ctcctcctgg ttgccccgca ccagccgtac gccgtccagg
     5461 aggcgggcga gttgggggtc cgcgtcgaag acctccgtcg ggctgccctt gaactcgtac
     5521 gcgggcgcgt cccgtttgcc gactcggcac tcgaagacgt cgcccaggca gaccacttcg
     5581 tccacatcct gtgcgcggat ccgttccagg accgcgcgca ggcgtgccgt gtccccgtgg
     5641 atgtcggtca gcacggcgtg gcgcaccgct ctcacccgct cgtcccgatc cgcacccggg
     5701 acgccaactc ctcgcagatc cgggctgctt ctgcggcgtc ggggccgtcg gcgatgacgt
     5761 atcccgcgcg gtcccgcgag gcgcgtaccc ggtggaccgg gtcgccgacg gcgacgtcca
     5821 ggtggatctc ggcggtggtg ccggtggtgt ccggcagggt gatgtgttcg acctttccgg
     5881 ggggtggggt gaggaagcgg atcgccgctc cccggcgggg caccggcggg gccgtgggtg
     5941 cgctcagcag gccgaacggg caggccgcgg tcagggtcac cacgtccaag tcgtaggctc
     6001 tgcgcaggag ttcgcggatc ttgtcgccgc cgatgcggtt gtgggactcc aggacgcgcg
     6061 ggcccttcgc ggtgaccttg acctcggtgt gggcggggcc ctccaccagc cctacggcgt
     6121 ccaggaaggc gtggaccagg tcggtcacct ccgccagtac gggcgcgggg agggcgctgg
     6181 gctgggtgtg gcccgtctcc acgaacgcgg ggccgaccag cttgtcggtg accgcgagga
     6241 ccgtgtgctt cccgtcgtgg gagaagctct ccacgctgat ctcggggccg tcgaggtact
     6301 cctcggcgag gggctgggtg ccgcccgccg cctcgaacgc ccgccaggcc tcctcggccg
     6361 cctgcgcgtc ggccaccttg aagaccgcct gactgccggt gccggccgtg ggcttgagga
     6421 ccacggggcc gtccgtcgta cggcagaagg cggccaggtc cgccgcgtcg cgcggcgcgg
     6481 cggtgtgcac cgggccgatg ccccgggcgt tgagcagttc ccgcatgcgc tgcttgtcct
     6541 ggagcaggcg cacggtgtgc agggagtttc cgggcaggcc gagccgttcg gcgagcgcgg
     6601 ccgtgggcag caggccgcgc tcggtgagcg acagcacccg gccgaagggg tgctcggcgt
     6661 gcagcggggc cagggccgcg tgcagcgcct cggtgtccgc caggtctgcc gcgatggccg
     6721 tgtcggcctc cgcggcgacg ggcggcgccg gttccgaggc gtcgtgcacg aagaccgtgg
     6781 agatgcccaa cgaccgtgcc gcgcacacca gttgcggagt gcccgcgagc accgcgagac
     6841 gcggtgtcgc gggtacctgg cgaccggagg tcatgccacg ggctccgccg ctgcccggcc
     6901 cgggagtgcc acggactccg gccggccgcc ggcggtcttg gcggccacct cgtcggggtc
     6961 gccggcggtc gtggcggcca cctcgtcgga gtcgccggcg gtcttggcgg ccagctcgtc
     7021 ggagtcgccg gcggtcttgg cggccacctc gtcggagtcg ccggcggtcc tggcggccac
     7081 ctcgtcggag tcgccggcgg tcctggcggc cagctcgtcg gggtcgccga agcgctcctt
     7141 gaggtacgcg atgatgtccg cgttctcccg cagcctgccc gggatcacgg tggcgccgtc
     7201 gaccagggcc gggatgaact tgctgcccgt ggtgcggatc agctcgtgcc gctcctcgcg
     7261 cggcttgggc acgttgatgt tgatgtagga gacctggagc ctggtgagca gttcgcgtac
     7321 cggcttgcag tcggggcagg tctcccgctg gaacaggacg agcatcagcg gccctcctcg
     7381 tggaacgtac ggatgatctc gccgacgccg gagcccggcg tgaaggtgtg gcccaggtcc
     7441 agcagggcca tctccagggc gccgaagatc ccgcacaggt cgagggcgtc ggcggcgccc
     7501 acgtgtccca cccggatgag cttgcccttg aggtgggcct ggccgccggt catcgtgacg
     7561 ccgtaggacg tcgacagcgt cttcagcagg cggccggtgt cgatgccctc gggggcgagg
     7621 gccgaggtga tgacgtggct gcgccggtgc ggggcctgca cgaaggtgct gaacccgagc
     7681 ttgagcaggg cgcgttcgga ggccttgccg agtacggcgt ggcgggcgcg gaaggactcc
     7741 gggccctcct cgttcagcat cttcagcgac tcgtgcaggg cgagcagcag ggacaccgcg
     7801 ggggaggagg acgacatggg gtagaagtgg cgcaggcgct cgaacgacca gtagtagttc
     7861 gggccgttgg ccttctccac cgccgtccac gccttctcgg agagggcgac gaaggccagc
     7921 cccggcggga gcatgaaccc cttgtgggag gcggcgagga ccacgtccag gccccacgcg
     7981 tccatctcca gcgggtgcac cagcagaccg ctgatggcgt ccacgaccac gaccacatcg
     8041 gtgtcgcgga agatccgccc gatcgcctcg acgtcgttga gcgcgcccgt ggaggtctcg
     8101 ctgtagacga cgaacgcgcc cttgatccgg gggtgttcgc ggtacgcgac cgccacccgc
     8161 tcggggtcgg cgctggtgcc ccagtcgctc tgcacgacgt gcacggtgag cccgtaggac
     8221 cggcagatcg cctggaagcg ttcgccgaag tagccgttgg agacgaccag gacctcgtcg
     8281 ccgggcgaga agcagttggc gaccgcggcc tccatcgcgc cggtcccgga ggaggcgatg
     8341 gtgtacaccg gggcgtcggt gccgaacagc gggggcagag cgccgctgat ctcgtggatc
     8401 agctgctcca tctcggtgga gcggtggtgg atgatctcgc gggccccggc cagcagcagg
     8461 cgctgcggta cctcggtggg gccgggggtg gccagccgca gcttcaccgg ctgttctcct
     8521 tgatctcgtc ggggctgtgg gcgggcgtcg cgggccgggg cgcgtcgagg aagacgcggc
     8581 gggtggcggg cttcaggtcg aagtcgaaga gcaccacgcg gtggctgggc gacatggcga
     8641 ggcggccgtc ccggaagggg acgagcagac tggtgccgag cagcgacgac ttcaggtgcg
     8701 agaagccgtt ggggtcggcg gtggtcagct cgtgcaaata gcggccgccg tcggcggggg
     8761 actccggcgc gatccgctcc agggcgcgca gcagatcctg cacggcgccc ggttcgtagc
     8821 gcatcacggt gagcccgatg gtgctgccgt gcgcgaacac cccggccaga ccgtcgccgg
     8881 tgccgtgctc ctggagagcg gcggcgatcc ggtcggtgag gtcgtagtgg gagttgaggc
     8941 cggtgctctc caccgtcagt tcgatcaccg ggccaccccc gcgggtccgg cgatcgcggg
     9001 cggcacgttc tcggtggtca ggtcgcccgg accggtcacc gtccaggcgg gcaccccgga
     9061 caggatgccg gtgatgcggt cgcgctgggc cgcgtgggcg gcgcggtggt cgtcgaggta
     9121 gtggtgccag cccgcgttcg cgccgtcgaa cgccgactcc acgttggcgc gcagcacttc
     9181 ggcgtgctcc tcacggctgc cggtgagcgg ctcgacggcc gtgcgctcgc cggggccgat
     9241 cgccgggtgc aggagcgcgg cgaccggtac gcgcaccccg accggggcgc tcgggaagcg
     9301 gtcccggaac ggcagtgggt ccaccgcgaa cttccccagc ggcgagaacg cgtggccctc
     9361 ggcgggcgtg tagtcgtcgc cgatgcccgc gatctccttc aggcccgggt acttggcgat
     9421 ggtgccgtag cccagatgcg ggtagtccgg ccagcccgcg gcgacgaccg agccgtccgg
     9481 cagctcgtgc agcacggtct tgtcgccgct gagcacctgg tagccgaagt gctccaccag
     9541 ctccatgagc accgtgctct tgccggcccc cttcgcgccg gtgaccagga cgacctcgcc
     9601 gtcccggtag gcggcggtgg cgtgcaggac cgcggcaccg gtgttctcct ggtgcctgag
     9661 caccaggtcg cgcaccagct cgacgaggtc catgtgcccg cccgcgccga gcgtcacgtc
     9721 gatcacgcgg gcggcgcggt cgaagcggaa gcggctgccc gtcttgacgc actccaggta
     9781 ctcgcggccg tccacggccg cccgccgcgc cgggacggtg aagaactcgc tcgcgctctt
     9841 gcgcaggtag atgtcctccg tcgcggtccc ggccgggggc gcgaggcgct ccgggtccgg
     9901 ggcgtggacg cgcagggtgg cgaccgggtc gcggtcgctc tcgccgggct tggcctcctc
     9961 ggcgacgaga tgggtggcca ggaaggcgag cgcgccgtcg atgtcggcgc cgggcgccac
    10021 ctccaggtcg aggtgggtgc cgtggaagtc cagccgcagg gcggcgtatt cggtcgtggc
    10081 ggtcactgtg cggcctccgc gagttcgcgt ccgacggact cgtagcgctc ctgctgctcc
    10141 agcatctggg gcatgcgctg gaggtcgaag acctccttga gcggggcgag ttcggactcc
    10201 acgttcagcg tggtgccgtc ccgcatgatc cgggtgaggg tctcccgcat ggcccggatc
    10261 gagctgcgca gcgcctggtt ggcgtagatc gccatggcga agccgcgctg ctccagctcc
    10321 tcgacggtga cctggttgta cgtggtcggc accacgatca ccgggaggtc gccgcggtag
    10381 gactcgcgga aggcgaacac ctcctcgggg tccttgcgct tggagtggat cagcagggcg
    10441 tccgccccgg cccgctcgta cacctcggcc cggcgcagcg cctccgccat gccctgcccg
    10501 gagatcagcg cctccagccg ggcgaccacc accatgtcgg tacgggtctc ggcggcggcc
    10561 cggatcttgg ccgcgaagtc gtccagcggg gcgaggtcct ggttgccctc gacgaagctg
    10621 ttgagcttgg ggaactgctt gtcctcgatg cacacgccgg ccacgccgcg cgcctcgtag
    10681 gagcgcacca tgtggatgac gttgttgacg ttgccgaacc cggagtcgca gtccgcgagg
    10741 accgggatgt ccacggcggc ggcgaggtcg gcggccgcgt ccaggcattc gcccatggcg
    10801 aggatgttgg cgtcgggcac cccggccgcc gccgagatct ccagtccgct ggaccagacg
    10861 acgtcgaacc cggcctcctc ggcgaggcgg gcgctgagcg ggctgtgcgc gcccatggca
    10921 cgggccagcc gcggcgcggc gagcgcggcg cgcagccgcc gggcggcgcc agccgtcggg
    10981 tcgttcggtt cctgctgggg cggtcggctc ccggtcatgc gtcccctccg tcggtccgtt
    11041 cggtttcctc ggtgcggccg gtggtcgccc cggccagttc ccggcacacg tcctcgaccg
    11101 tctcgtcgag gaccttgcgc gggcgtgccg cgccgacccg gcgcccgacc tgccacggat
    11161 cgagaccgtg gcgggcgctg atcttgtcga tgcggtcctg gaagtagaag tggtggtcgt
    11221 tgatcccggc ggcgatctcg gcccggcgca ccgtcatggg gcgcggcagc acctgctcgg
    11281 ccacgtactc ggcggcctcg cagaccagcg gcagatcggc gtgggtggcg tacttgggcc
    11341 acgcggtgag gaaggcggcg gcctgctcgg tggcgaggtt cccggcgccc cgcgccatgg
    11401 cgcacagcga ggcgtccagc caggtcacgc ccgcgtccag accggcgagc gcgttggccg
    11461 cggccaggcc caggttgttg tgggcgtgga tgccgagcgg agtcggcagc cgctcggcca
    11521 gcagctcgac gcacccgcgc acgtcgtccg gggtgagcgc gccgaaggag tcggcgatgt
    11581 agaagacgtc cggtgcctcg gccgccacga tcccggcgat ctcggcgagt tgttcctcgg
    11641 tggtgtagct gacggccatc agattgacgc cgaccttgag gccgagggcg cgcacggcgc
    11701 ggacgtactc cgggaccccg tcgatgttcc acgggtaggc ggcgacgcgc accatggaca
    11761 cggggctgtc gggcaggtcg tccatggccc ggaccgggca cacggtgggg acgaccatca
    11821 cggccagctc ggcgtgggac acgtccggca gcgcggcgag gtactcgggt gtgcagtgcg
    11881 ccgaggggcc cgcggtggcg ctggccttgc tgccggagcc gccgcggtag ccgacctcga
    11941 tgacgtcgac gcgggcggcg tccagggtgg cgacgatggt gcggacgtcg gtggcggtga
    12001 actgccagtc gttctggttc ccgccgtcgc gcagggtgca gtcgagcagg gtgggcttca
    12061 cacggctcct tccaggtgcg acgtcaacgg gcccgcggac ggggcatcgg cgccctccgc
    12121 caggtgggtg cggaaacgtt ccaccagctc ctgcggcgtc tgttccacgc gcttgcccgc
    12181 cgggtggccg cgcagctcgc agcggatccg caccagcgtg ggtgtgccgg tcaggtccag
    12241 ggcggccagc ccgtcgaggt cgtccacggt gtgggcggcg gcgtaaccgg ccgcgagggc
    12301 catggccgcc gggtcgccga gggcggcggt gcgctggccg ccggtgctct cgtgggcgcc
    12361 gttgtcgagg acgacgtgca ccaggttcgg ccggtgctcg gcgatcatcg gcagacagcc
    12421 cgggttcatg gcgaacgagc cgtcgccgtc cagcaccagc acccggcggt ccgggtgggc
    12481 cacggcgatg ccgagcccga tcggcgcggc catgcccatg gagccgacga ggtagaagtg
    12541 gttggcacgg tcgcggatgt tgtacacgtc gcgcgtgatg tagccgcagg tgctcaccgt
    12601 cagggcggcc ggctcccgct ccagcagcgc gcccagggcg tcacggatgt ccagcaacgc
    12661 tagatcccct cccggacgat cagtacggcg gtgctccggc cgtcgtcgta ctgcttgagg
    12721 aacgcctcaa ccgagtcctc ggggtgcgcg ttgtccagca ccgtccacgg cagcccgaac
    12781 acatcgagca gccgggtgag ttcgcgtccg atgacgtcgt gctcgacggc gtcgttgccg
    12841 ccgtgtccgc gccagctgac cacgagcggc agatggacgt cgtagatgag gttgaacgag
    12901 gtgaggacgt tgagcgagta gccgaggccg gagttctgca tcagaaccac gggccgggcg
    12961 cccgccacgg cgagtccgga ggcgactccg agggcgctgt cctcgcgcgg cgcggggatg
    13021 tacgcggccg gacagccggg gtcctccagt aaccggaaga agcccttcag cagggaacac
    13081 gggacgccgg tgaagtggga atgcccgcgg gttacgaagg cgttcagcag gatttctgcg
    13141 acttcggtcc cgggtcctgc cgccaacgat gacttgggca gcacggatga cctttctttt
    13201 gcgaagcacg gacgcggtca cgcccggagg ggagcgtctg tgtgcccccc gaccgagcgg
    13261 atcctgcatg acgcccaggt gaatccgcaa tgacattcca gtatgcgagc aggcgaattc
    13321 cagaacgggc gattcgggta tgtcaggcgt tcggtgatct tgtgggtggt cactgcatgg
    13381 atttgagccc cgcaatgact caatatcgat gtttgaaacg gtgggcatga ggatccggtg
    13441 ccggaattcg gttgccatcg acacaagtag gagggactgg ttccggtctc gggacgggct
    13501 tcgccgctgg tcacgcgggc gtacgtgcgt gacgcgcatg cgcgcgcgtg cccggaggcg
    13561 agggctcggg ggcattcgtg ccctgcccgg ccggctgcgc gttttcgccc attggaggcg
    13621 ggttcggggc gtgtgagagg ccggaatccg gcgttcggtg catctggttt gagtcagatc
    13681 ggaggtgggg tgtccgaaat ttgacgggct gaatttgatc ttcctcgtgt acattcggcg
    13741 ccatcggtgt gtcgatgcct cagggggccc gaagaatcgg ggaggctcgc agaagagcat
    13801 tgacggcggc gttcggcccg gtccgacgca tagccccgca cagcgtcgtg cgggagagcc
    13861 gactcctcgc ttcgttcatt tctcaagttc ctttcttaat ttcttttgag ctctttccga
    13921 catgcaacgg ggttgattca tgcgcgcggc agaacaggac agtggtgtcg gccttgtcga
    13981 cgacggagcg caggccgcgg tggaggcggc cgaggaaatc atcgaggagg cgtccggcgg
    14041 cgacggcggg gtgttcgccc cggcgtggtc ggacgaccgg gtggcgcacc tgctggagcg
    14101 gctcacctcg gacttcgtgc tcgacaacac ctaccgcgag cagcacctga gcctcacggc
    14161 cagcgagaac tacccgagca agctggtccg gatgctcggc agcgccctgc agggcggctt
    14221 ctacgagttc gccccgccgt atcccaccga ggcgggcgag tgggccttcc cggactccgg
    14281 cgcgaacgcg tccctggtgg gcaagctgac cgggatcggg cggcagctct tcgaggcggc
    14341 caccttcgac tggcggccca acggcggctc ggtcgccgag caggcggtac tgctgggcac
    14401 ctgcgggcgc ggtgacggct tcgtgcactt cgcgcacaag gacgggggcc acttcgcgct
    14461 ggagtcgctg gccgcggcgg cgggcgtcaa cacctaccac ctgccgatgg tcgaccgcac
    14521 cctgctcatc gacgtggacc ggctggccac gctgtgcgcc gagcacccgg agatcaagct
    14581 cgtcatcctc gaccagtcct tcaagctgcg ctggcagccg ctcgcccaga tccgggccgc
    14641 gctgcccgag ggcgtcttcc tcgcctacga cgccagccac gacggcgcgc tgatcgcggg
    14701 cggggcgctg ccgcagccga cgctgctcgg cgcggacgcg gtgcacggca acacccacaa
    14761 gaccatcgcc gggccgcaga aggcgtacat cgccttccgg gacgccgacc acccaaggct
    14821 cagggcggtg tcggactggg tctgcccgca gatgcagagc aattcccacg ccgagctgat
    14881 cgcgccgatg tacgtggccc tctccgagat cgccctgtac ggccacgact acgcccgcca
    14941 gatcctcgcc aacgccaagg ccctcgcgca cgccctgcac gaggagggcg tacgggtctc
    15001 cggggagtcc ttcggcttca ccgagacgca ccaggtgcac ctcgtcaccg gctcggccgc
    15061 cgacgcgctg cgcctggccc tcggcgacct ggcgcacgcc ggcatccgca ccaccaacat
    15121 cgaggtaccg ggcgccaacg gcctgcacgg ccttcgcctc ggcgtccagg cgatgaccag
    15181 gcgcgggctg cgcgagacgg agatgcgcga ggtggcccgc ctggtggcca aggtcgtcct
    15241 gcgccgcgcc gagccgacga cggtgcgcgc cgaggtggcc gacctcctcc agtcccaccc
    15301 cctggaccgg ctcgcgttct ccttcgactc ccacgtcgag tccccggcgg cggcccggct
    15361 gctcggggag gtgttccggt gagcgtcacg agtacgcggg acaaggggcg cacccggacc
    15421 ttgatccctc agcctcccct tccacccgga gtgcccggcg tgctgagcgc ggccgtgcgc
    15481 ccccgccgga acgacgtacc gcacgccgtc atcagcgcgg aggtctggca caccgagtgg
    15541 gcgagggacc tggtcgagcg gctggcgctg cggacggtca tcgccgacga gcggctgtcc
    15601 tgcgagcggg tgctggacac gctcgccgcg cagtccgtgg cgttcgtctg cgaggaactg
    15661 ccggtcagcg gactggccgc gatgacctgg gcggcgcgcc gcaccgaggc gctgcgccgg
    15721 gacgacccgc tgctcggcgt ggtgttcatg gtcgaggacc cccgggccct ggacccccgc
    15781 gccctcggcc cgctggtacg caccctgtca cccggcgaca cggacctgcc cggcttcctc
    15841 gccgaagtgc agcgcgtgcg gcgcgagttc cactgggagg cacgcaaccg gcgccccgct
    15901 tcccggccgg ccggagaggc cgcggcgcgc cccgcccggt gggccgggac cgtaccgaac
    15961 ttcaatgtga agcacccgtc catgctgctg ccgtacgccc tgctctcggc gcgcacgcgg
    16021 cgggaggtct actgcgaggt ctcgccgcag gaggccctgc tgttcgtcgc ggacagcgtg
    16081 ggccggggcc cggccgccgc cgtcgccgag gtcttccgca tcctgcggca ggacgtcgac
    16141 cggctcaact ccgcgctcgg ctcccggctt cggctgcacc tcgaccacgc cgacgactac
    16201 gaggtggtcc aggccgcctg cgaggccggg ttcgactccg tcatggtcga cgggtccggc
    16261 cgtacgctcc accagaacgt gctgttcacc aacgcggcca ccgaactggc ccgccgctac
    16321 ggcgtcctcg tcgagggcga gatcggctcc atcgacggcg cgggtctgcg caagtggaac
    16381 aagacgacac cgggcgactt cacccggttc gccgaggaca ccgacctcga tctgatcggg
    16441 gcgcacgtgg gccagttcca cagcttcgac tacggcttcg ccgccacccg ccgctcgctc
    16501 gccgaggtcg gactgctgga cgaccgcgcc accggcgacg acctgcacgc cttcaccgag
    16561 gcgtgcgcga gcgtcgagaa ggagctcaac gacgccggac tgcccggcta ctcggccgag
    16621 caccggctgc tcgcggcgct ggcccgccgg gccaccaccg agggggacgg gccgcgcctg
    16681 gacgccgtgt tccaggagat gcgcgcccgg gtcccggtgt accaacggcc gctgctggac
    16741 gctctggaga aggagtggct ggagcggcgg ctggccaagg cgcgccgcaa gaccgaactg
    16801 tgggacgagg tcttcgccac cgagcccgcg cgcgagcacc ggtacgcggc gatcgaccac
    16861 ggactcgtcc agaccctcac caaagtgctg gacggcaccg gccgcgccct ggtcgtgcac
    16921 ggcggcacct cgctctcccg cgtcgacctc gcctccctcc ccgcgcacgg cgtcgcccgg
    16981 gtgaacttcg gcagcggcgt ctacgccgac tatctggagg ccctgcgcgc acacctcccc
    17041 gttgacgtca acgcggcaac gggcgaggga acttggaagc acctgcggcg catgtcgtac
    17101 cttgcggagg cgacacggga ctggcggtcc tggaccgacg ctccgccgtc cttcgtcgac
    17161 accttcgtca gccggctgga gcggtgctac gcggccccgc tccgtggccg tacgcccgaa
    17221 caggcccagg gaggccgcag ttgagtccct ttgagcagat cgtcgacctt ctcgaccgtg
    17281 agaaggccag gtaccgcgtg gtcgagcacc cggccgaggg gcgctccgag gaggtcgcct
    17341 cggtgcgcgg cacgaccgtc tcgcagggcg cgaaggcgct ggtgtgccgg gtgaagggca
    17401 tcgaggcgcc ggcggtgctg gccgtgctcg cgggcgaccg gcgcgcggac ctgaagaagg
    17461 tcgtcgccgc cgtcgagggc aagaagggcg ggttcgcgcc ctcggagctg gccgagcagc
    17521 tcaccgggtg cgcggtcggc accatcccgc cgctgtcgct gaccggcacg ctgcccgtcg
    17581 tcgtggacga ggagttcctc gcggcccacg aggagatcgc cttcaacgcg gggcggctgg
    17641 accgctcggt ggtcatggcc accgaggact acgtacggat cgtggcgccc gcggtggcgt
    17701 ccatcgcggc gccggtggtt ccggcggcgg ctcagtaggg accgacacaa gccctgcgga
    17761 gtccgggcac aggcccggca gggtccggca caaggcccgg cagggtcgga agaaaaaagg
    17821 ggggtgcgtc ggcttgcgtg tgcgcatcgg ggcacagttc gtcgcgctgg cgtgcgcgtg
    17881 gggctccagt ttcctgttca tcaaggtcgc gctggacggg ctgacccccg cacaggtcgt
    17941 ctgggggcgg ctggtgctgg gcgcgttggc gctgtgcgcg gtcatggcgg tgacccgcag
    18001 ccggatcccc cgcgaccccg ggctctggct gcggctgtcc gtggtctccg tcctgctgtg
    18061 tgtcgtcccc ttcctcctct tctcctgggc cgaacagcac ctcgactccg gtatggcgag
    18121 catcctcaac gccaccaccc cgctgctgac cctcgcggtc agcacggcgg ccctggcgga
    18181 cgaa
//
