LOCUS       NZ_CP104794            10942 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4348686
            Orig. end                         :: 4359628
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10942
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="14"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10942
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10942
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5942](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5942)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     tRNA            185..268
                     /anticodon=(pos:4348905..4348907,aa:Leu,seq:cag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="N6J21_RS19445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Leu"
     gene            185..268
                     /locus_tag="N6J21_RS19445"
     misc_feature    380..395
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     gene            complement(413..1633)
                     /locus_tag="N6J21_RS19450"
     CDS             complement(413..1633)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (62-405). E-value:
                     1.7e-50. Score: 164.1. Matches aSDomain:
                     nrpspksdomains_N6J21_RS19450_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FabF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659170.1"
                     /locus_tag="N6J21_RS19450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="WP_093725670.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.2e-49, bitscore:
                     168.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="FabF (E-value: 1.1e-141, bitscore: 471.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPHTDDVLVTGVGALTPLGATADSSWAGLLAGRSGVRDLGPDWP
                     EELPVRIAGTVEADLTASIGRARARKLDRGEQLAVVAAREAWQDAGVPQVEPERLAVVV
                     GTGIGGVLSTLGQDDLYESSGMRRLSPYAVPMLMPNGPAAWVSMELGARAGARAPVSAC
                     ASGAESLALGLDLIRAGRADVVVAGGTEATLHPFMIAAFAQMKALSTQAGDPAAVSRPF
                     DAARSGFVIAEGAALFVLERADFARARGARVYGALAGAAVHSSAHHITASDAEGQVQAM
                     RTALADAGVEPRDIGHVHAHATSTPSGDIAEAEALTKVVGEETAVTATKSMTGHMLGAS
                     GAFGAMSALLSLHHGVVPAVRNLDEQDPEVRLDVVRGENRTGSWDAALANSFGFGGHNV
                     SLVMKRA"
     aSDomain        complement(419..1447)
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS19450_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.70E-50"
                     /label="N6J21_RS19450_PKS_KS.1"
                     /locus_tag="N6J21_RS19450"
                     /protein_end="405"
                     /protein_start="62"
                     /score="164.1"
                     /tool="antismash"
                     /translation="SIGRARARKLDRGEQLAVVAAREAWQDAGVPQVEPERLAVVVGTG
                     IGGVLSTLGQDDLYESSGMRRLSPYAVPMLMPNGPAAWVSMELGARAGARAPVSACASG
                     AESLALGLDLIRAGRADVVVAGGTEATLHPFMIAAFAQMKALSTQAGDPAAVSRPFDAA
                     RSGFVIAEGAALFVLERADFARARGARVYGALAGAAVHSSAHHITASDAEGQVQAMRTA
                     LADAGVEPRDIGHVHAHATSTPSGDIAEAEALTKVVGEETAVTATKSMTGHMLGASGAF
                     GAMSALLSLHHGVVPAVRNLDEQDPEVRLDVVRGENRTGSWDAALANSFGFGGHNVSLV
                     MKR"
     misc_feature    2370..2385
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            complement(2683..3669)
                     /locus_tag="N6J21_RS19455"
     CDS             complement(2683..3669)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /locus_tag="N6J21_RS19455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_261718925.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.5e-05, bitscore:
                     23.0, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPTRRILITGGTGFVGSHVAALLADGPNAANAPRLRLLVHHRPT
                     TPDDTGRVEVRRGSLTDPRSLRGVCDGVDTVLHLASLIGGSPADCAAVNDAGTGALLEE
                     AARAGVERIVQLGTTAVYRDEPHTGAREGEPVLGPSSPTSVSRLAGEERVLAAGGVVLR
                     PHLVYGPGDVWVVPALVDFLTAFPRWIDGGRARTSLIGVHDLARAVAALSLRPELPRGQ
                     VLHAARPDPVPVRDLVGAVCRELRLPLPEGEVSLARAAALTGADRDPLVRRRLSLLAVD
                     HWYDSSRLWQLLDMAPLDDFARDFAVGAAWYRSLLARRTDGELFSTR"
     gene            3850..4614
                     /locus_tag="N6J21_RS19460"
     CDS             3850..4614
                     /NRPS_PKS="Domain: PKS_KR (12-171). E-value: 7.7e-14.
                     Score: 43.9. Matches aSDomain:
                     nrpspksdomains_N6J21_RS19460_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659168.1"
                     /locus_tag="N6J21_RS19460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_261718926.1"
                     /sec_met_domain="adh_short (E-value: 9.1e-34, bitscore:
                     115.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.5e-29, bitscore:
                     101.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYGGLLQGKHAFITGASSGIGAAAARVFCREGAAVTLAARREER
                     LAALVDELRAEGGKAQYVVLDVAVKAQVEAGFARALESFGRLDVAFNNAGVGAEGTPMH
                     LMGDEVYDTVMDTNVRGMWNCLRQEVAAMLEHGGSIVNTSSTAGLVATPVAAPYIAAKH
                     AVIGLTKAVAAEYSASGIRVNAIVPGATHSEMLDAWLERIPGVAEEMARAALLPRIAVP
                     EEIAEAAAWLSSDRASFVVGATVPVDGGWTVR"
     aSDomain        3886..4362
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS19460_PKS_KR.1"
                     /evalue="7.70E-14"
                     /label="N6J21_RS19460_PKS_KR.1"
                     /locus_tag="N6J21_RS19460"
                     /protein_end="171"
                     /protein_start="12"
                     /score="43.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="FITGASSGIGAAAARVFCREGAAVTLAARREERLAALVDELRAEG
                     GKAQYVVLDVAVKAQVEAGFARALESFGRLDVAFNNAGVGAEGTPMHLMGDEVYDTVMD
                     TNVRGMWNCLRQEVAAMLEHGGSIVNTSSTAGLVATPVAAPYIAAKHAVIGLTKA"
     gene            complement(5001..5942)
                     /locus_tag="N6J21_RS19465"
     CDS             complement(5001..5942)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="N6J21_RS19465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_261718927.1"
                     /sec_met_domain="AfsA (E-value: 4.4e-18, bitscore: 63.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFPVRPVAPVRPHILPPATAVDTRLVHRTDRKDVLPTGITKLDEG
                     HYTVFTQWPADHRRFTDQDGNFLPSLVIESIRQAVILVAHDGLGVPLGHQFLVSTAHHR
                     IDPALLLAPGAPDRLELDVSVHDLKHRRGIPSALEARVVIRADGEIIGTGGGECSVMTP
                     EVYRRLRRGRTETAWPAALAAPLPAPLPPHSVGRTHACDVALSAGDAPGEWLLRADVSN
                     PLMFDHPNDHMPAMVLLDAAQQAAHLTFGPRPFRPFSCVVSCSRYVEFDAPCTVRTDLT
                     EETDGGWTLSVTGHQGGEQVFEILFRGSAGTR"
     misc_feature    complement(6077..6092)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.13"
                     /tool="antismash"
     misc_feature    6083..6096
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.4"
                     /tool="antismash"
     gene            6163..6828
                     /locus_tag="N6J21_RS19470"
     CDS             6163..6828
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985136.1"
                     /locus_tag="N6J21_RS19470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_093725282.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRQAILEAAGEVFAECGYAATRISDVYQRCGVTKGA
                     FYFHFTSKAELAQAVLDEQVADQIRYFAPPQEPGAPKLQEWVDVTLLVAHRLTFDRMLQ
                     GSIRLAVDQGHDVIDRKVPYQAWIEVSTRLLGEAQERGELVPGIDVATVSEAAVGAFTG
                     VQLLSEIMTNRADVEERIAVLYDLMARSLARPEVYGRLDITAGRGKALSRGAAVSGSV"
     gene            6875..7192
                     /locus_tag="N6J21_RS19475"
     CDS             6875..7192
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035381.1"
                     /locus_tag="N6J21_RS19475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_261718928.1"
                     /transl_table=11
                     /translation="MQIQQLRAFREVAAELSVTRAARNLNYAQSTVTAQIQGLEESVGA
                     ALFDRSHRRLALTEAGARLLPYARIIIDAAEEGRRAVAAAQATPRCDARVRRVRRARLP
                     S"
     gene            7746..9134
                     /locus_tag="N6J21_RS19480"
     CDS             7746..9134
                     /EC_number="2.5.1.54"
                     /GO_function="GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659164.1"
                     /locus_tag="N6J21_RS19480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class II 3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="WP_261718929.1"
                     /transl_table=11
                     /translation="MNADQRMTDVDSWRRFPAAQQPEWPDPDALKGVLAELATYPPLVF
                     AGECDQLRERIARVARGEAFLLQGGDCAETFDAITADQVRDKVKTLLQMAAVLTYATSV
                     PVVKIGRIAGQYSKPRSKPVEVRGDITLPVYRGDAVNGQEFTEEARRPDPERLKRMYHA
                     SASTLNLIRAFVTGGYGDLRQVHEWNRDFVNSSPVGARYEALARDIDRALTFLAACGAD
                     PGEFHLAEFYSSHEALLLDYEAALTRIDSRTGHAYDVSGHMVWIGERTRQLDHAHVEFA
                     ASVRNPVAVKLGPSATRDEVLGLIDRLDPEREPGRLSFITRMGRDRVRDVLPALVEMVT
                     EAEAPVAWICDPMHGNTFSAASGHKTRHFDDVLDEVTGFFEAHRALGTHPGGIHIELTG
                     DDVTECVGGGHPIGLTDLHRRYQTACDPRLNRYQALDLAFQVAELYADRAVPAVTGPRL
                     ARVA"
     misc_feature    9205..9219
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
     gene            9301..10290
                     /locus_tag="N6J21_RS19485"
     CDS             9301..10290
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1015: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /locus_tag="N6J21_RS19485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_093725288.1"
                     /transl_table=11
                     /translation="MQRVSEVRPRQVRRCTADPPPAERGGLQIVRNHEEILEGTTVRHR
                     FGEVTVSLVQGGPRELVRPGRLIADGRRARLRVCRPLRGEVWVFQDGRHGVVRPPQLIS
                     FDTGRPFKVIMPEEFRMVDVMVTHSLVGLTATDTQRLTARAWNGDQGVAALLSGMLEGL
                     DRHGEEVETAVDLLGGSVAGLTAVLFAERMREVAPDSEIARQALMLRIQAHVREHLAEP
                     SLSPNEVARRHNISLRYLQKIFQEFGVSPARWIRDERLARCRTELADPALDHLPVALIG
                     ERAGLYGASHFSRLFRGRYGVTPSEFRKERRAMHHLDSDTVSNAGARS"
     gene            10287..10496
                     /locus_tag="N6J21_RS19490"
     CDS             10287..10496
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="N6J21_RS19490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_093725290.1"
                     /transl_table=11
                     /translation="MSAAGTAPEAVYGTVEHFLGLLLPGGLPPESACPGRLVSARTVTH
                     QGGPAPRAAGRGERRTRSGRGRTT"
ORIGIN
        1 gatacgtgaa cagacacacg ctgagacgtg cgggacggct tcgcgatgcg gtgcctccgg
       61 ttccggtgca ggtgagcggc tgccccggcg gaagggatgt gaatccaccc cgtccagcgt
      121 gctaatgttc tgggtgtcgg aaggcgccgg accgcaagga acggggcccg aggacacacc
      181 caatgcgcgg gtggcggaat aggcagacgc gctggattca ggttccagtg cccgcaaggg
      241 cgtgggggtt caactccccc ctcgcgcacc gctgagaggg cggcatcgat cagatgccgc
      301 cctttctcgt tgtccatggt gactgtgacg gttctctcgc ccggtcccct ctctctcctc
      361 acccccgggc ccgcccgcgt gtgaggaagc tctcaggtca tcgccgtatc tctcaggccc
      421 gcttcatgac caggctcacg ttgtggccgc cgaatccgaa ggagttggcg agggctgcgt
      481 cccaggagcc ggtgcggttc tcgccgcgca ccacgtccag acggacctcg gggtcctgct
      541 cgtcgaggtt gcggaccgca gggaccaccc cgtggtgcag cgagagcagc gcggacatgg
      601 cgccgaaggc gccggaggcg cccagcatgt ggccggtcat ggacttggtg gcggtcaccg
      661 ccgtctcctc gccgacgacc ttcgtcagcg cctccgcctc cgcgatgtcc ccgctcgggg
      721 tggaggtggc gtgcgcgtgg acgtggccga tgtcccgggg ctcgacgccc gcgtcggcca
      781 gcgccgtgcg catggcctgt acctggccct ccgcgtcgga ggcggtgatg tggtgcgcgc
      841 tggagtgcac ggcggcgccc gcgagcgcgc cgtacacccg tgccccgcgc gcccgcgcga
      901 agtcggcgcg ctccaggacg aacagggcgg cgccctcggc gatcacgaag ccggagcggg
      961 ccgcgtcgaa cgggcgggac accgccgccg ggtcgcccgc ttgtgtggac agggccttca
     1021 tctgcgcgaa cgcggcgatc atgaacgggt gcagggtggc ctcggtgccg cccgcgacga
     1081 cgacatcggc gcggcccgcc cggatcaggt ccaggcccag cgccagcgac tcggcaccgg
     1141 acgcacaggc gctcacgggg gcgcgggctc cggcccgggc gcccagctcc atggagaccc
     1201 aggcggccgg gccgttgggc atcagcatgg gcaccgcgta cggggacagc cgccgcatcc
     1261 cggaggactc gtagaggtcg tcctggccga gcgtgctgag gacgccgccg atgccggtgc
     1321 cgacgaccac ggcgagccgc tccggctcca cctgcggcac gcccgcgtcc tgccaggcct
     1381 cgcgggcggc gaccacggcc agctgctcgc cccggtccag cttgcgggcc cgcgcccggc
     1441 cgatcgatgc ggtgagatcg gcctcgacgg tcccggcgat acgcaccggc agctcctcgg
     1501 gccagtccgg gccgaggtcg cggacaccgg agcgtccggc cagcaggccg gcccaggagg
     1561 agtcggcggt cgcacccagg ggtgtcagcg cgcccacacc cgtcaccagt acgtcgtcag
     1621 tgtgcggggc catctgtcgt ctcctcgatc cgcggggcag tcgtcgatac gtcccttcac
     1681 catggcccgg ccggtggccg ggacggcccg tggggcgcgg accggcgaga gaccgtgcgt
     1741 ggatgtggca ccggggttgt gttgggcggt ccgtgggttg gctcgggccg ttctgggccg
     1801 tcggtctgcc tgggcccgtg ccgggctgtc tgtcggtctg cccgggggcg tgccgggcgg
     1861 gtgcgcggtg cgcgacggcg tacgtgcggg gtggtggctg cggcggcctg tggggcggga
     1921 gcgggcggcg atcctgccgg acccgtgtcg tggggcttgg cggtctgccc ggggcgccgg
     1981 gggcccggcg gcgttcggtg cggcggcgtg cggctgggcg gtgggcgcgg tggcccgtgg
     2041 ggcgcggacc ggcgagagac cgtgcgtgga tgtggcaccg gggtcgtgct gtgggggccg
     2101 tgggtcggtc cgagcgtggg ccggtgggcg cggtggcccg tggggtgcgg acgggcgcgg
     2161 ggtcctgtgt gagcgtggcg ctgaggtcgg gccgtggggt ctggcggtct gcccgatccg
     2221 tgccgtgcgg gacgttggtc tgtcagagcg ggggtgggtg gcgttcggtg cggcgggcgt
     2281 gcgtgccggg tggtggttgc ggcggtctgt ggggcgggag cgggcgaggg gtcctgtgtg
     2341 agcgtggcgc cggggttggg ccgtggggtc tggcggtctg gtcggacccg ggccgggtgg
     2401 ttcgtcggac ggcccggggt gcggggcggg tggcgtccgc tcctgccgct acgtgccggg
     2461 ccgtgggccc cgtggcctgg ggcggggacg ggcaagcgcc gtgtgggggg tggggcggac
     2521 cccggagggg caatgcggcg ttccgggtgg gtggggggtg tgcgcggatg tgttactgcg
     2581 tgtgcgctgg tgggcgagga gatcgttggg tgggcgccta gcgtgggctc ggtgctgcgg
     2641 cgcccccgcc gggccgggga ctggcccggc gggagccggt cgtcatcgcg tggagaacag
     2701 ctccccgtcc gtgcggcggg ccagcagcga ccggtaccag gcggcgccca cggcgaagtc
     2761 acgggcgaag tcgtccaggg gcgccatgtc gagcaactgc cacagacggg agctgtcgta
     2821 ccagtggtcg accgccagca acgacagacg ccgccggacc agggggtccc ggtcggcgcc
     2881 ggtcagggcg gccgcccggg cgagggagac ctcgccctcg ggcagcggga gccgcagctc
     2941 gcggcagacc gccccgacca ggtcccgcac cggcaccgga tccggccggg cggcgtgcag
     3001 gacctgaccg cgcggcagct cgggccgcag ggacagcgcg gccacggccc gggccaggtc
     3061 gtgcacaccg atcagcgacg tgcgggcccg cccgccgtcg atccaccggg ggaaggcggt
     3121 gaggaaatcc accagggccg gtaccaccca gacgtcgccg gggccgtaca ccaggtgcgg
     3181 gcgcaggacg acgccgccgg ccgcgaggac ccgttcctcc ccggccagcc tgctgacgct
     3241 ggtgggcgag gacggcccga gcaccggttc gccctcccgg gccccggtgt gcggctcgtc
     3301 ccggtacacg gccgtcgtgc ccagctggac gatccgctcg acccccgcgc gagcggcctc
     3361 ctccaacagg gccccggtcc cggcgtcgtt gacggccgcg cagtccgccg ggctgccgcc
     3421 gatcagcgag gccaggtgga gcacggtgtc cacgccgtcg cagaccccgc gcagcgagcg
     3481 cgggtcggtg agggagccgc ggcgcacctc caccctgccg gtgtcgtcgg gcgtcgtcgg
     3541 gcggtggtgg accagcaggc gcagccgtgg agcgttcgcc gcgttcgggc cgtcggccag
     3601 cagcgcggcc acgtgggagc ccacgaagcc ggtgccgccg gtgatgagta tccgtcgcgt
     3661 cggtgccatg cgaatttctt ccgatcttgt ctcgggaaca gtgatcaggc caggcaatgc
     3721 ggaacggagg ggggagaaaa cggcgcgtgg ggggtgccgg tggccctgtg gaaagggccg
     3781 ggaaatggat tcgagtcaga tttgagtgcg acgcgaatca gggacttaaa tcacgggtaa
     3841 tgtgaggcca tgcgctacgg cggacttctg cagggaaagc acgcgttcat cacgggcgcc
     3901 agcagtggga tcggggccgc ggcggcccgg gtcttctgcc gggagggagc cgcggtcacc
     3961 ctggcggccc gccgcgagga gcggctcgcc gcactggtgg acgagttgcg cgccgagggc
     4021 ggcaaagccc agtacgtggt gctcgacgtg gcggtgaaag cgcaggtcga ggcggggttc
     4081 gcgcgggcct tggagagctt cgggcggctc gatgtggcgt tcaacaacgc cggggtcggg
     4141 gccgagggaa cgccgatgca cctgatgggc gacgaggtct acgacaccgt catggacacc
     4201 aatgtccgcg gaatgtggaa ttgcctgcgt caggaagtcg cggcgatgct ggagcacggt
     4261 ggctccattg tcaacacgag cagtacggcg gggcttgtgg cgaccccggt tgctgcccct
     4321 tacattgccg ccaagcatgc cgtcatcgga ttgaccaaag cggtcgcagc ggaatattcg
     4381 gcgagtggaa ttcgggtcaa tgcgattgtg cccggggcga cgcacagcga aatgctggac
     4441 gcgtggctgg agcggattcc cggggtcgcg gaggaaatgg cgcgggcggc gctgctgccg
     4501 cggatcgcgg tgccggagga gatcgccgag gcggcggcct ggctgagcag tgaccgggcc
     4561 tcgttcgtgg tcggcgcgac ggtgccggtg gacggcgggt ggacggtgcg gtaagcggcg
     4621 gcgagtcggt ggggctgcgt cggtgcgggt catgggacga gctcctgagc tgtggcggaa
     4681 ctgaaggtgc ggtgcccggc gctgggccgg gggtgagcga agggcggtgc cggtggaggg
     4741 cggaagcgga cgggaccgtg cgcggatcca cgtatgccgg ggtacggatg tgcggatcgg
     4801 gcgccgggag cggttgggtg cgggcggcgg gcgggcgcgg gaacggtgtg cgggccgggt
     4861 ggccggacgg tttccggcgc ggggcgggcg acggggccga tggtgcgcgt actagagccg
     4921 atggtgcgcg gatgagaggt gcggcgctcg ttgccgacgg caggcccacc cacctgcggg
     4981 cccggtggtc gcccggcgga ctagcgggtc ccggccgagc cgcggaaaag gatctcgaag
     5041 acctgctcac cgccctggtg gccggtgacg gacagcgtcc atccgccgtc ggtctcctcg
     5101 gtgaggtcgg tccggacggt gcagggggcg tcgaactcga cgtagcgcga gcaggagacg
     5161 acgcaggaga agggacggaa ggggcgaggt ccgaaggtca ggtgcgcggc ctgctgagcg
     5221 gcgtcgagga ggaccatcgc gggcatgtgg tcgttcgggt ggtcgaacat cagcgggttc
     5281 gatacatcgg cccgcagcag ccactcgccg ggtgcgtccc cggcggacag ggcgacatcg
     5341 caggcgtggg tgcggccgac ggagtgcggc ggcaggggtg cgggcagcgg cgcggccagt
     5401 gcggccggcc aggcggtctc ggtacggccg cggcgcagtc ggcggtagac ctcgggggtc
     5461 atgacggagc actccccgcc gccggtgccg atgatctcgc cgtcggcgcg gatgaccacc
     5521 cgggcctcca gggcggacgg gatgccgcgg cggtgcttca ggtcgtggac ggatacgtcg
     5581 agttcgagcc ggtcgggcgc gccgggggcg agcagcaggg ccgggtcgat gcggtggtgg
     5641 gcggtggaga cgaggaactg atggccgagc gggaccccga ggccgtcgtg cgcgaccagg
     5701 atcacggcct ggcgtatgga ctcgatgacc agtgagggca ggaagttgcc gtcctggtcg
     5761 gtgaagcggc ggtggtccgc gggccattgg gtgaagacgg tgtagtggcc ctcgtcgagc
     5821 ttcgtgatgc cggtcggcag cacgtccttg cggtcggtgc ggtgtacgag cctggtgtcg
     5881 acggccgtcg cgggcggcag gatgtgcggc ctgaccggtg cgaccggcct gaccggaaac
     5941 accgtcctcc tgatcccccc gtggcggcgt cggcccctgt gaataccgat gttcgtggtc
     6001 attgctcttc cttctggaac gagaaggtgc atgacgggtg actggttcca accccgccgg
     6061 taagatacgc gcatgcgggt atgtttttca atgagagcgg gtggttccgc ataagcgccc
     6121 gctctggaca ctccatcaac tacgacgaag gaagggggcc cagtggcaca gcaggatcga
     6181 gccatcagga cacgtcaggc gattctggag gcggccggtg aggtcttcgc cgagtgcggc
     6241 tacgccgcca ccaggatctc ggacgtctac cagcggtgcg gtgtgacgaa gggcgccttc
     6301 tacttccact tcacgtccaa ggccgagctg gcgcaggcgg tgctggacga gcaagtggcg
     6361 gaccagatcc ggtacttcgc cccgccgcag gagcccggag cgcccaagct ccaggagtgg
     6421 gtggacgtca ccctgctcgt ggcgcaccgc ctcaccttcg accgaatgct ccagggcagc
     6481 atccgcctcg cggtggacca ggggcacgac gtcatcgacc gcaaggtgcc gtaccaggcc
     6541 tggatcgagg tgagcacccg gctgctgggg gaggcccagg agcggggcga actggtgccg
     6601 gggatcgatg tggcgaccgt gtccgaggcg gcggtcggcg ccttcaccgg ggtgcagctg
     6661 ctgtcggaga tcatgacgaa ccgggcggac gtggaggagc ggatcgccgt tctctacgac
     6721 ctgatggccc ggtccctggc ccggcccgag gtgtacggcc ggctcgacat caccgcggga
     6781 cgcggcaagg cgctctcgcg cggggccgcg gtctccggct ccgtctgagg cgttttccgg
     6841 gcggccccgc cgggccggcc gaagggagcg gcacatgcag atccagcagc tacgggcatt
     6901 tcgcgaggtg gcggccgagt tgagcgtgac tcgggcggcc cgcaacctca actacgcgca
     6961 gtcgacggtg acggcgcaga tccaggggct tgaggagtcg gtgggggcgg cgctgttcga
     7021 ccgcagtcac cggcggctgg cgctgaccga ggcgggggcg cggctgctgc cgtacgcccg
     7081 gatcatcatc gacgcggcgg aggaggggcg gcgagcggtg gccgcggcac aggcaacgcc
     7141 ccgctgcgac gcgcgggttc gccgggtgcg gcgggcgcgg ctgccgtcgt agccgttgcg
     7201 gtgtcgtcgt gcttgtgcct gacgtagccg tcgcggtgtc ggcgtgcttg tgcctgacgt
     7261 agccgtcgcg gtgtcggcgt ggctgttccc ggcgtagttg tcggggtgcc gtcgtagctg
     7321 tgcggaactg gtccttcatg tggttgccga cgattgggga gtgactggaa gagattcttg
     7381 tgctggaact gggtaagccc gacgccctcg gaatgtgaca ttccgggggc gttgtccgtt
     7441 cgcgcgtatt tcggcaaggg gcggtgcgcg gtgcggcaac cgggcgggcg gggacgttcg
     7501 tagagtcgtg gcatcgcggt gagtccgggg ccgttgacgg gccggatgcc gctcgccccc
     7561 cgagtcgcgg ccctcccccc gaccggcgca ctcctcgtac atcccccggc gaggagtgtc
     7621 ccggcggcgg gagggccgct ccgtaccccg gagccgtgac gtacccggag cccgtgacgt
     7681 cgcgggcgat ccgccgcaca cccgatccga caccttcccc ggacacaagt gaggcacagc
     7741 tcaacatgaa tgctgatcag cgcatgacgg acgtggactc ctggcgccgc tttcccgccg
     7801 cccagcagcc cgaatggccc gacccggacg ccctcaaggg ggtgctggcg gagctggcga
     7861 cctacccgcc gctggtgttc gccggggagt gcgaccaact gcgcgagcgc atcgcgcggg
     7921 tggccagggg cgaggcgttc ctgctccagg gcggcgactg cgccgagacc ttcgacgcga
     7981 tcaccgccga ccaggtgcgg gacaaggtca agacgctgct gcagatggcg gccgtgctga
     8041 cctacgccac ctcggtgccg gtcgtgaaga tcggcaggat cgccgggcag tactccaaac
     8101 cgcgctcgaa gcccgtcgag gtgcgcggcg acatcaccct gcccgtctac cgcggcgacg
     8161 ccgtcaacgg acaggagttc accgaggagg cccgccggcc agacccggaa cgcctcaaac
     8221 ggatgtacca cgcctccgcc tccaccctga acctgatccg ggccttcgtg accggcggct
     8281 acggcgatct gcgccaggtc cacgagtgga accgcgactt cgtcaacagc tcccccgtcg
     8341 gcgcccgtta cgaggcgctc gcccgggaca tcgaccgcgc cctgaccttc ctggccgcct
     8401 gcggggcgga ccccggggag ttccatctcg cggagttcta ctccagccac gaggcgctgc
     8461 tgctggacta cgaggcggcc ctgacccgga tcgactcgcg caccgggcac gcctacgacg
     8521 tctccgggca catggtgtgg atcggtgagc ggacccggca actggaccac gcgcacgtgg
     8581 agttcgcggc ctccgtgcgc aaccccgtcg cggtcaagct cggtccgtcg gcgacccggg
     8641 acgaggtgct cgggctgatc gaccggctcg accccgaacg ggagccgggg cggctgtcgt
     8701 tcatcacccg catgggccgg gaccgggtgc gcgacgtgct gcccgcgctg gtggagatgg
     8761 tgaccgaggc cgaggcgccg gtcgcctgga tctgcgaccc catgcacggc aacaccttct
     8821 ccgccgccag cggccacaag acgcggcact tcgacgacgt actggacgag gtgaccggct
     8881 tcttcgaggc gcaccgggcg ctgggcaccc accccggcgg tattcacatc gagctgaccg
     8941 gggacgacgt caccgagtgc gtgggcggcg gccacccgat cggtctgacc gacctgcacc
     9001 ggcgctacca gaccgcctgc gacccccggc tcaaccgcta ccaggcgctc gatctcgcct
     9061 tccaggtcgc ggagttgtac gccgaccgcg cggtgccggc ggtgaccggg ccgcggctgg
     9121 cgcgggtggc ctgagctgcg ctctctcggt gaggccgggc cgatggcggt ccggcctcgg
     9181 cggtgtgccc gcctccggtg atccatcgaa actctcgatc gaagtagaaa acccgcatgc
     9241 gggtatgttt ccctggtggt gcttcgacga gagctccgac atcggaacag ggggtacgcg
     9301 atgcaacgtg tcagtgaggt gaggccgcgg caggtgcgcc ggtgcaccgc cgatccaccg
     9361 cccgccgagc gcggcggact gcagatcgtc aggaaccacg aggagatcct cgaagggacc
     9421 acggtccggc accgcttcgg cgaggtcacc gtatcgctgg tgcagggcgg tccccgcgaa
     9481 ctggtccggc ccggccggct catcgcggac gggcgccgcg cccgcctcag ggtgtgccgt
     9541 ccgctgcgcg gcgaggtctg ggtcttccag gacggacggc acggcgtcgt acggccgccg
     9601 cagctcatct ccttcgacac cgggcgcccg ttcaaggtga tcatgccgga ggagttccgg
     9661 atggtcgacg tcatggtgac gcactccctg gtcggtctca ccgccaccga cacccagcgg
     9721 ctcaccgccc gcgcctggaa cggcgaccag ggcgtcgccg cgctgctgag cggcatgctc
     9781 gaagggctcg accggcacgg ggaggaagtg gagacggccg tcgatctgct cggcgggagc
     9841 gtcgccggac tgaccgcggt gctcttcgcc gagcggatgc gcgaggtcgc gcccgacagc
     9901 gagatcgccc ggcaggcgct gatgctgcgg atccaggccc atgtgcgcga gcacctcgcc
     9961 gagccgagtc tgagccccaa cgaggtggcc cggcggcaca acatctcgct gcgctacctc
    10021 cagaagatct tccaggagtt cggggtcagc ccggcccgct ggatccgcga cgagcggctc
    10081 gcccgctgcc ggaccgagct ggccgacccc gccctcgacc acctgcccgt ggcgctgatc
    10141 ggcgaacggg cgggcctgta cggcgcctcc cacttcagcc ggctcttccg cggccgttac
    10201 ggggtcaccc ccagtgagtt ccggaaggag cgccgcgcca tgcaccacct ggacagcgac
    10261 accgtttcga atgccggagc gcggtcgtga gcgcggcggg taccgccccg gaggcggtgt
    10321 acgggacggt ggagcacttc ctcgggctgc tgctgccggg cggactgccg ccggagtcgg
    10381 cctgccccgg gcggctggtg tcggcgcgga cggtgaccca ccagggcggc ccggcgccgc
    10441 gtgcggcggg gcgcggtgag cggcgcaccc gctccggacg cgggaggacg acatgaccgc
    10501 gaccgccacc gggctgtccg aactgcggat caaggtgctc gggccgctgg agatccacgt
    10561 cggcggcgag ccgcgtaccc tcaccgcgcc gatggcgcgg cgggcgctgg ccgtactgct
    10621 gctggacgcc aaccacctgg tctccgtgca ggccctcgtc gaggaactgt gggacgagga
    10681 gccgccgcgg ctggcccgca agaccgtgca gacctacatc taccaactgc gccaggccct
    10741 caagggcagc gccgacggcg acaccgggga gcggctggag accggttccg gcgggtaccg
    10801 gctgaccgcg cggccggggg agctggacct gtgggagttc gagcagcggg tgaaccgggc
    10861 ccgggccgcg ctggcctccg gggcgccgga ggacgcggcg cggctgctgc gcgaagggct
    10921 cgcgctgtgg cgcggggagc cg
//
