LOCUS       NZ_CP104794            10471 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2988493
            Orig. end                         :: 2998964
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10471
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="8"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10471
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10471
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5471](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5471)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    16..29
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.73"
                     /tool="antismash"
     misc_feature    49..62
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.25"
                     /tool="antismash"
     misc_feature    complement(50..64)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.83"
                     /tool="antismash"
     gene            166..1329
                     /locus_tag="N6J21_RS13235"
     CDS             166..1329
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997728.1"
                     /locus_tag="N6J21_RS13235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_255310314.1"
                     /transl_table=11
                     /translation="MFRRAYLTTANKVAFATVALLVVGSLTLVAADVLAQPLSRRTLIT
                     GIILLLLLPAVLLGQYAPPPYRLPYRWRWVLLGLQTLLTYLPVLVFEHRWLSLLGFLAG
                     AVLLTVPRPASVVIALAVVASGPALVHSSLVEANRSSMSVLASTLITGSAVYAVVHLSL
                     LAARLQASQAQAARLAEYRERTRIARNLHDLIGSSLVSIAVQSESALRRTPRSTPAYEA
                     LSEIASLARRSHSEVRSITSARESSQLHSELAHAQRLLSSAGVVSRTTLPPQLTLPGAV
                     SGCLSAVLREAVSNVVQHSQATRCEIEVTASDSTIRLSVDNDGLRSAVRETRTGSGLES
                     LRLRTLALSGVLRTEVSGGHFVLTAELPLDPPVALGDTDRVHESACV"
     gene            complement(1270..1872)
                     /locus_tag="N6J21_RS13240"
     CDS             complement(1270..1872)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717663.1"
                     /locus_tag="N6J21_RS13240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_261718432.1"
                     /transl_table=11
                     /translation="MISVLVVEDMQVVRAGLVALLDGEFGIEAAGQATDAVEALRLAER
                     LRPDVALLDIDLPGMDGLALARELPRRVPDCRTVMLTALDRPGHVRRALDAGASGYLLK
                     SVSPRVLAEAVRTAVAGGRVIEPPELAEELGSPSPLTPAEAEVLRMAAGGADARAIAAE
                     LFFSVGTVRNRLSAAVGKLHARTLVDAVRIAERHGWI"
     gene            1965..2561
                     /locus_tag="N6J21_RS13245"
     CDS             1965..2561
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521621.1"
                     /locus_tag="N6J21_RS13245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261718433.1"
                     /transl_table=11
                     /translation="MGLDITVIIADPSPFTPFPPQKRLSHLRATLLADDTGLWGIDAPC
                     VEEGWVWPEGANSDRFALYEFPGTLGSYKPHFWAGERWDEVREQVDPPLRDELDALLDG
                     LLGPEDRCTDADLFGEPGPGVLLARSPERVRELAAVWERVRPQLHRVREPFGRASFPDT
                     GWITTYDEFASLLTGWGHVLAEGERRGWCVVGLSG"
     gene            2671..3630
                     /locus_tag="N6J21_RS13250"
     CDS             2671..3630
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="N6J21_RS13250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_261718434.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 4.8e-52, bitscore:
                     174.6, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLRCAVLDDFQQVATTLADWSPIAEDVEVVSFGVHFDNEDALAA
                     ALAEFDIVVTLRERVPFPASLFERLPRLRLLIASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVRESDSLRSGGPWQSTVGVDLCGRRLGLLGLGKIGSRVAQ
                     VGHAFGMRVSAWSQNLSEERAAEVGVERAGSKEDLLAGSDFVSIHLALSDRTRGLIGAR
                     EFGLLKPTAYLVNTSRSAIVDQDALLDALRAGRIAGAGVDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSQDNYRTYYGQAVENIRAYLAGSPVRQLP"
     gene            3723..4013
                     /locus_tag="N6J21_RS13255"
     CDS             3723..4013
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818707.1"
                     /locus_tag="N6J21_RS13255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_093722214.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVAVGTGAMVVGGAGMALADAGADGKAAHSPGVASGN
                     LIQVPVNVPVNLCGNTVDVIALLNPAFGNTCVNNGDHDRGHGGHGNGGYGD"
     gene            complement(4151..5017)
                     /locus_tag="N6J21_RS13260"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="N6J21_RS13260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_261718435.1"
                     /transl_table=11
                     /translation="MAYTRKDVTKLTRTERRRLVNAFLEIKRSGEYDEFVRTHIDHYVS
                     DGEGGLRTAHMTPSFLPWHRQFLLELERALRRVDSSVTLPYWDWTKDRKAGAAPWTDDL
                     LGGTGRITDRQVMTGPFAYSAGNWTIRESITDARFLTRDLGRRRDPIELPTRSDLEWAM
                     SDPVYDSSPWDSTSRKGFRNKLEGWGAGRGPVAWRNHNRVHRWVGGDMVGGASVNDPVF
                     WLHHAFVDLCWTRWQKRHRNHRYLPAKPPGIGDEQRGRIVARHQKLPPWDVTPDQLEDV
                     SRIYRYA"
     gene            complement(5001..5471)
                     /locus_tag="N6J21_RS13265"
     CDS             complement(5001..5471)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660312.1"
                     /locus_tag="N6J21_RS13265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase cofactor"
                     /protein_id="WP_261718436.1"
                     /sec_met_domain="melC (E-value: 7.1e-47, bitscore: 157.9,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGVGGVAVGAEPGARGARPVAARGGRRDLLRGLLAAAGALTLA
                     PVVAASRPTAVARGGDAPGELAFDETYRGRHIRGIHHAGAPVGAAQWHVTVDGVRLHLM
                     RRADGTWLSMVDHYRSYATPLDAARAAVDELGPGQRLRDSGTDTGDHHGVHA"
     misc_feature    5608..5621
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.44"
                     /tool="antismash"
     gene            complement(5631..6143)
                     /locus_tag="N6J21_RS13270"
     CDS             complement(5631..6143)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="N6J21_RS13270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_093722217.1"
                     /transl_table=11
                     /translation="MKSTTRGTLVAVVTGVAAAVAGAAPAAAVGTVPVPVPLDGVEKSL
                     NMELPRIAGELPVPMPGAPEGPRYVEGRLIPERVLPQLPVTGGLPGLDVRSPLPHLLGD
                     DFDHVGVDAPASDLRTLAPGLTLDAPLTPPNPDNFGLPDPKLPQVGLLAPVLQTVTGAD
                     VAAGPGL"
     gene            complement(6275..6883)
                     /locus_tag="N6J21_RS13275"
     CDS             complement(6275..6883)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660310.1"
                     /locus_tag="N6J21_RS13275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_261718437.1"
                     /transl_table=11
                     /translation="MTTAAGRPERRSVGAGWGFALLLVLTGAAGLLASWVITLDKFKLL
                     EDPDFTPGCSLNPVVSCGSVMESDQAEAFGFPNPMLGLVAYGIVICVGVGLLAGARFPR
                     WYWLCFWAGCLFGVGFVSWLQFESLYRISALCLWCCLAWIATIVLFWYVTSLLVTRELV
                     PAPGWAVTLFAEFTWVLPVLHLGIVGMLILTRWWDFWTS"
     gene            complement(6883..7380)
                     /locus_tag="N6J21_RS13280"
     CDS             complement(6883..7380)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="N6J21_RS13280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5949 family protein"
                     /protein_id="WP_093722219.1"
                     /transl_table=11
                     /translation="MTSTSSENRAFSAAELGTLVVMAWSGEAPDGDMPYLLAYTLGDGT
                     AGPEGSTRAVEALLENNGLPVGGELVDGATKPSLPVSLLVESGHAVVSMTGFNAQCAPP
                     PEWLAAVGERGFAYFVFTTRPWPEAEPGKPVDPQALADFAGATETLDAAAHVVLPARSL
                     RG"
     gene            complement(7566..7859)
                     /locus_tag="N6J21_RS13285"
     CDS             complement(7566..7859)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136064.1"
                     /locus_tag="N6J21_RS13285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_093722220.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVVAGAAAPAFAAHSPADLTPTSLNGAVNTLA
                     SGNFTADDALPLQHQSQALDTENKDSVLNTVNGVTTALNQGGGLLGGLPLPS"
     gene            complement(7994..8698)
                     /locus_tag="N6J21_RS13290"
     CDS             complement(7994..8698)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660307.1"
                     /locus_tag="N6J21_RS13290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261718438.1"
                     /transl_table=11
                     /translation="MRALPARRIAVSALSATLLLGIAGPAASAVDDSARERTHAVSAAP
                     VPGADALLAQTKSLGDLGGVLTPVTDLLNAVLKADNGQLSAAEAEKLGQAVKDAIAKAT
                     AAAPVTPPVSTPELPTTPQLPTTATLPVANGDNKSAQLPADLVADALAGLQKEVDALLA
                     AAVKGDLVKVTGAVSAVLTSLVNVVAATLLGAGLPAPTLPGLPQLPSLPSLPGAPELPS
                     TPELPVGGGLLP"
     gene            complement(8947..9186)
                     /locus_tag="N6J21_RS13295"
     CDS             complement(8947..9186)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="N6J21_RS13295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chaplin"
                     /protein_id="WP_093722222.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAAAAVAGGAGIAAADAGAGGGAAHSPGVLSGN
                     VVQVPVHVPVNVCGNTVNVIGLLNPAFGNTCVND"
     misc_feature    complement(9984..9993)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 tcccttgaag agttcagaac atacgggcgc ccgatccgat cacgctcaag atcatcaaaa
       61 aagtgagatc ttcatcggtc tccgaagcaa cctgccgcct ctctgaatgc tcatgtagtg
      121 ggggaagtct gcccgcacac gggacagacc tgagaaagga gaacagtgtt tcgccgggca
      181 tacctgacca ccgcgaacaa ggtcgcgttc gccaccgtcg ccctcctggt cgtcggctcg
      241 ctcacgctgg tggccgcgga tgtcctggct caacccctga gcagaagaac gctgatcacc
      301 gggatcatcc tgctgctcct tctcccggcc gtgctgctgg ggcagtacgc gccaccgccc
      361 taccgtcttc cctaccggtg gcgctgggtg ctgctgggcc tccagacact gctcacctat
      421 ctgccggtcc tcgtcttcga gcaccgctgg ctgagcctgc tgggcttcct ggccggcgcc
      481 gtcctgctca ccgtgccccg cccggcctcc gtggtgatcg cgctcgccgt cgtcgccagc
      541 ggtcccgcgc tggtgcacag cagcctggtg gaggcgaacc gcagcagcat gagcgtgctc
      601 gcgtccacgc tgatcacggg gagcgccgtg tacgcggtgg tgcacctctc cctcctggcc
      661 gcccgcctcc aggcctcgca ggcccaggcc gcgcgcctcg ccgagtaccg cgagcgcacc
      721 cgcatcgccc ggaatctgca cgacctgatc ggctcctcac tggtgtccat agccgtgcag
      781 agcgagagcg ccctgcgccg caccccgcgc tccacccccg cgtacgaggc gctgagcgag
      841 atcgcctcgc tcgcccggcg gtcccacagc gaggtgcgca gcatcaccag tgcgcgcgag
      901 tcctcccagc tccactcgga actcgcccac gcccagcgcc tgctgtccag cgccggcgtg
      961 gtcagccgca ccactctgcc gccgcagctg accctccccg gagcggtgag cggctgcctg
     1021 agcgccgtgc tgcgggaggc cgtcagcaat gtcgtccagc acagccaggc gacccggtgc
     1081 gagatcgagg tgacggcttc ggacagcacg atccggctgt ccgtcgacaa cgacggcctg
     1141 cgctccgcgg tgcgggagac ccggacgggc agtggtctgg agagcctgag gctgcgcacc
     1201 ctggccctga gcggcgtcct gcgtacggag gtcagcggtg ggcacttcgt actgaccgcc
     1261 gagcttccgc tagatccacc cgtggcgctc ggcgatacgg accgcgtcca cgagagtgcg
     1321 tgcgtgtagc ttgcccaccg cggcggacag ccggttgcgc accgtgccca cactgaagaa
     1381 cagttccgcc gcgatggccc tggcgtccgc cccgcccgcg gccatccgga gcacctcggc
     1441 ctcggccggc gtcagaggac tcggcgagcc cagttcctcg gccagctcgg gcggctcgat
     1501 cacccgccct ccggcgaccg ccgtccgcac cgcctccgcc agcacgcggg gcgagacgct
     1561 cttcagcaga tagcccgacg cgcccgcgtc cagggcgcga cggacgtggc ccggcctgtc
     1621 cagggccgtg agcatcacgg tgcggcagtc cggcacccgc cgcggcagct cccgtgccag
     1681 cgccagcccg tccatgcccg gcagatcgat gtcgagcagt gcgacatccg gccggaggcg
     1741 ttcggcgagc cgcagagcct ccaccgcgtc ggtcgcctgg cccgcggcct cgatgccgaa
     1801 ctcgccgtcg aggagagcca ccagtccggc cctcaccacc tgcatgtcct ccacaacgag
     1861 cacgctgatc acgaattgaa cactccggtc cgctcactcc ggggggcaca cccggtctcc
     1921 ggcattcggg gcccggtcgt gtcccctcgc taccctcccc catcatgggc ctcgacataa
     1981 ccgtgatcat cgccgatccc tccccgttca ccccgtttcc tccgcagaag cgcctctcgc
     2041 acctccgcgc caccctgctc gcggacgaca ccggcctgtg gggtatcgac gcgccttgcg
     2101 tcgaggaggg gtgggtgtgg cccgaggggg cgaacagcga ccgcttcgcg ctgtacgagt
     2161 ttcccggcac cctcggctcc tacaaaccgc acttctgggc cggggagcgg tgggacgagg
     2221 tacgggagca ggtggaccct ccgctgcggg acgaactgga cgcgttgctg gacgggctcc
     2281 tcgggccgga ggaccgctgc accgatgcgg acctgttcgg ggagcccggc cccggcgtac
     2341 tgctcgcacg gtcaccggag cgcgtacggg agctggccgc ggtctgggag cgcgtgcgtc
     2401 ctcaactcca ccgggtgcgg gagccgttcg gccgggcatc gttcccggac accggctgga
     2461 tcaccaccta cgacgagttc gcgagcctgc tgacgggctg ggggcacgtc ctggccgaag
     2521 gggaacggcg cgggtggtgc gtcgtgggcc tgagcggata gaaccctccg ggcgaggccc
     2581 ccgtagagtc ggcacgcccc agcccccaac cctcaaaccc ccagccctca gcccacagcc
     2641 cacagcccac agcccacgaa aggatccccc gtgcccctcc gctgcgccgt gctcgacgac
     2701 tttcagcagg tcgcgaccac gctcgccgac tggtcgccga tcgccgagga cgtcgaagtg
     2761 gtgtccttcg gagtgcactt cgacaatgag gacgccctcg ccgccgcgct cgccgagttc
     2821 gacatcgtcg tgaccctgcg cgagcgggtg ccctttcccg cctccctgtt cgagcggctc
     2881 ccccggctgc ggctgctcat cgcctccggt atgcgcaaca gcgtcatcga ctacgcagcc
     2941 gccgaggccc acggggtcac cgtctgcggt accgccagct cctccacccc gcccgtcgaa
     3001 ctgacctggg cgctgctgct gggactggcc cgggggattg tgcgggagag cgactccctg
     3061 cgttccggtg ggccctggca gtccaccgtc ggggtcgatc tgtgcgggcg tcggctgggg
     3121 ctgctcgggc tcgggaagat cggcagccgg gtcgcccagg tcgggcacgc cttcgggatg
     3181 cgggtcagtg cctggagtca gaacctcagc gaggaacggg cggccgaggt gggggtggag
     3241 cgggccggtt cgaaggagga cctgctcgcg ggcagcgact ttgtctccat tcacctcgcg
     3301 ctcagtgacc ggacccgcgg gctgatcggg gcccgggagt tcgggctgct caagccgacc
     3361 gcctacctcg tcaacacctc ccgctccgcc atcgtcgacc aggacgccct cctcgacgcc
     3421 ctgcgcgcgg gccggatcgc cggtgccgga gtcgacgtct tcgacatcga gccgctgccc
     3481 gccgatcacc cgatgcggac cgctccccgg ctcctggcca ctccccacct cgggtacgtc
     3541 tcccaggaca actaccgcac ctactacggc caggccgtcg agaacatccg tgcctacctc
     3601 gcgggctcac ccgtacggca actgccgtaa cacccgttac cctccgcgat cagcggcgtt
     3661 gaacgggaag tgcggcggcg ccaactgccg ccgccgagct caccgagtca aaggagaacg
     3721 tgatgtctcg catcgcgaag gcagccgctg tcgccgtcgg taccggtgcc atggtggtcg
     3781 gcggggccgg tatggccctg gcggacgcgg gcgcagacgg caaggccgcc cactcgcccg
     3841 gcgtcgcctc gggcaacctc atccaggtcc cggtcaacgt tcccgtcaac ctgtgcggga
     3901 acaccgtcga cgtgatcgcc ctgctgaacc cggccttcgg caacacctgc gtcaacaacg
     3961 gcgaccacga ccgcggacac ggcggtcacg gcaacggcgg ctacggcgac tgagaccgtt
     4021 ccccgtctcc acgacgccct tccgtcccgt ccgcgccttc cccaaccgga cgtgacgcac
     4081 gagagccccg gcaccctgag cgccggggct ctccccatgc gaaccatggg aaacatgtga
     4141 agtccgagaa ctacgcgtag cggtagatcc ggctcacgtc ctccagctga tccggtgtca
     4201 cgtcccacgg cggcagcttc tggtgccgcg ccacgatccg gccccgctgc tcgtcaccga
     4261 tccccggcgg tttcgccggg aggtagcggt ggttccggtg ccgcttctgc caccgcgtcc
     4321 agcacaggtc gacgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gacgcgccgc
     4381 ccaccatgtc cccgcccacc cagcggtgca cccggttgtg gttgcgccag gccaccggac
     4441 cgcgcccggc gccccagccc tccagcttgt tgcggaagcc cttgcgggac gtggagtccc
     4501 atggcgagct gtcgtagacc gggtccgaca tcgcccactc caggtcgctc ctggtcggca
     4561 gctcgatcgg gtcgcgccgc cggccgaggt cccgggtgag gaagcgggcg tcggtgatgc
     4621 tctcccgaat cgtccagttg cccgcggagt aggcgaacgg cccggtcatc acctgccggt
     4681 ccgtgatgcg cccggtgccg ccgagcagat cgtccgtcca cggcgccgcc ccggccttgc
     4741 ggtccttcgt ccagtcccag tacggcagcg tcaccgagga gtccacccgg cgcagcgccc
     4801 gctccagctc cagcaggaac tgccggtgcc agggcaggaa ggagggcgtc atgtgggccg
     4861 tacgcagtcc gccctcgccg tcggagacgt agtggtcgat gtgcgtgcgt acgaactcgt
     4921 cgtactcgcc gctccgtttg atctccagga acgcgttcac cagccgccgc cgctcggtgc
     4981 gggtcagctt ggtgacgtcc ttacgcgtgt acgccatggt ggtcccccgt gtccgttccg
     5041 ctgtcccgca gccgctgtcc cggccccagt tcgtccaccg cggcccgggc ggcgtccagc
     5101 ggggtggcgt aggagcggta gtggtcgacc atgctgagcc aggtgccgtc ggcccggcgc
     5161 atcagatgca gacgtacgcc gtcgacggtg acgtgccact gcgcggcgcc gaccggggcg
     5221 cccgcgtggt ggatgccgcg gatgtgccgg ccgcggtagg tctcgtcgaa ggccagttcg
     5281 ccgggggcgt ccccgccccg cgcgaccgcc gtcgggcggg aggccgcgac gaccggcgcc
     5341 agcgtgagcg ccccggccgc ggcgagcagc ccacgcagca ggtcccgccg tccaccgcgc
     5401 gccgccacgg gtctcgcccc ccgcgcgccc ggctccgccc ccacggccac gccaccgacg
     5461 cctacgacca tcgtgtgctc ctcatcaagt tggtttgtcg tagcggtaac ccccgggccc
     5521 ggctccgggt caccgcccgt gccccggaca gccgaagggc ccggccggcg tcgaacgccc
     5581 cgggcccacg gccgttgacc acgaatgatt tgtagatgac aacgaacgac ctacagaccc
     5641 ggccccgccg ccacgtccgc gccggtcacc gtctgcagga ccggggccag cagccccacc
     5701 tgcggcagct tggggtcggg caggccgaag ttgtccgggt tcggcggggt gagcggggcg
     5761 tccagggtca ggccgggcgc cagggtgcgc aggtcggagg cgggggcgtc cacgccgacg
     5821 tggtcgaagt cgtcgccgag gaggtgcggc agcgggctgc gcacgtcgag gccgggcagc
     5881 ccgccggtga ccgggagctg cggcaggacc cgctcgggga tgagccggcc ctcgacgtac
     5941 cgcgggccct cgggggcacc cggcatgggc accggcagct cgccggcgat cctcggcagc
     6001 tccatgttga gggacttctc cacgccgtcc agcggcaccg ggacgggaac cgtgccgacc
     6061 gcggcggcgg gtgcggcgcc ggcgaccgcg gcggccacgc cggtgactac ggcgacaagg
     6121 gttcctcggg tggtcgactt catttcggct acggtccctt ccaggggttc gttcggtgga
     6181 tcggcatccg ctccgcacaa cgactccggg atcacgacca gcgcacaaat tcccccgagt
     6241 gccgcagtag tacgacctac atccggtcag ctcgtcagct cgtccagaag tcccaccaac
     6301 gggtcaggat cagcattccg acgatgccga ggtgcagtac cggcagcacc caggtgaact
     6361 cggcgaacag cgtcaccgcc caccccggcg cgggcaccag ttcccgggtg acgagcagtg
     6421 aggtgacgta ccagaaaagg acgatggtgg cgatccacgc cagacagcac cacagacaca
     6481 gggcgctgat ccggtacagc gactcgaact gcagccagga cacgaacccg acgccgaaca
     6541 gacagcccgc ccagaagcag agccagtacc agcgcgggaa gcgggccccg gccagcaggc
     6601 cgacgccgac gcaaatgacg atgccgtagg cgaccaggcc cagcatcggg ttggggaacc
     6661 cgaaggcctc ggcctgatcg ctctccatca cgctgccgca ggacaccacc gggttcaggc
     6721 tgcatcccgg ggtgaagtcc gggtcctcca gcagcttgaa cttgtcgagg gtgatgaccc
     6781 aggaggccag cagcccggcg gcgccggtga gcaccagcag cagggcgaag ccccacccgg
     6841 cgcccacgga ccgccgttcg ggccgtcccg cggccgtggt catcagccgc gcaggctgcg
     6901 ggccggcagt acgacgtggg cggcggcgtc cagggtctcg gtggccccgg cgaagtcggc
     6961 cagcgcctgc gggtcgaccg gcttgccggg ctcggcctcg ggccaggggc gggtggtgaa
     7021 cacgaagtag gcgaagccgc gttcgccgac ggcggccagc cactccgggg gcggggcgca
     7081 ctgggcgttg aagccggtca tgctgacgac ggcgtggccg gactcgacga gcaggctgac
     7141 cgggaggctg ggcttggtcg ccccgtcgac gagttcaccg ccgaccggca gtccgttgtt
     7201 ctccaggagc gcttcgaccg cccgggtcga gccctcgggg cccgcggtgc cgtcgccgag
     7261 ggtgtaggcg agcaggtacg gcatgtcacc gtcgggcgcc tcgccgctcc aggccatcac
     7321 gacgagcgtg ccgagttcgg cggcgctgaa ggcgcggttt tcgcttgagg ttgaggtcac
     7381 cgcgcgacct tatcgacgcc ccggacggcc cccggggccc cgctcacccg accgggcgac
     7441 gccccgggcc ctgtccacac gaaagggtta cggcgccctc gaacagcccg gccgacatca
     7501 ccccgtcaaa acacctgggg ccggttccgt acggcacaga accggccctc gaagcacagg
     7561 agttgtcagc tcgggagcgg cagcccgccg agcagtccgc cgccctggtt cagcgcggtc
     7621 gtgacgccgt tgacggtgtt gagcacggag tccttgttct cggtgtcgag cgcctgcgac
     7681 tggtgctgga gcggcagcgc gtcgtccgcc gtgaagttgc cgctggcgag cgtgttcacg
     7741 gcgccgttga ggctggtcgg cgtgaggtcg gcggggctgt gcgcggcgaa cgcgggggcg
     7801 gctgctccgg cgacgaccag ggaaccggcg acgacagctg cggccttaag ggacttcatc
     7861 gtgttccttt cctaggcaac tgccgtgctt aacgaccttg ttcggaagcg gaaaccccgg
     7921 acctgcttcc tcatatgaaa tagccgttgt gccgaccggg cgggtcggca caacggctgt
     7981 gcggggacgg tgcctacggc agcaggccgc cgccgaccgg cagttcgggc gtgctcggca
     8041 gttccggtgc gcccggcagg ctcggcaggc tcggcagctg gggcaggccg ggcagggtcg
     8101 gggcgggcag cccggcgccg agcagggtcg cggcgaccac gttgaccagg ctggtcagca
     8161 cggcggagac ggcgccggtg accttgacca ggtcaccctt gaccgcagcc gccagcaggg
     8221 catcgacctc cttctggagg ccggccaggg cgtcggcgac gaggtcggcg gggagctgcg
     8281 cggacttgtt gtcgccattg gcgacgggca gcgtcgcagt cgtggggagc tgcggagtcg
     8341 tggggagctc cggagtcgac accggaggtg tgaccggcgc cgccgccgtc gccttggcga
     8401 tggcgtcctt caccgcctgg ccgagcttct cggcctccgc cgcggacagt tggccgttgt
     8461 cggccttgag tacggcgttc agcaggtcgg tgaccggggt gagcacaccg ccgaggtcgc
     8521 ccagggactt ggtctgggcg agcagcgcgt ccgcgccggg gacgggcgca gcggacaccg
     8581 cgtgggtgcg ctcacgggcg gagtcgtcga cggccgaggc ggccggcccg gcgattccga
     8641 gcagaagggt ggcgctgagt gcggaaaccg ctatacgccg tgcgggcagg gcacgcatgg
     8701 ggtgttcctt tcggtggtgc gtgggacttg cctccaccgt gcgaacgttc ctcacgcact
     8761 gcaaccgttc gagtgcacac cgttaccgga agcctgaggt gcgggctgca tcgtgcctgg
     8821 tgagaggcgc agcaaggcct tacgaccgac acgcccccgc agtcccccgt tcgggggtac
     8881 acgcaccggc cgcccctccg tggcggttcc acggaagagc ggccggtagc gggtctcgcg
     8941 ctgacgtcag tcgttgacgc aggtgttgcc gaacgccggg ttcagcagcc cgatgacgtt
     9001 gaccgtgttg ccgcagacgt tgaccggaac gtggaccggg acctggacga cgttgcccga
     9061 caggacgccc ggggagtggg cggcgccgcc gcccgcgccg gcgtcggcgg cagcgatacc
     9121 ggcgccgccc gcgacagccg cagcggcagc ggacgtaaga accaggccct tcgcgatacg
     9181 cgacatggag aggtgctcct tgggattgac acaaacaccc gggcggggat gcccggcgct
     9241 gtgtcaacgc gcgacgcgca cgggggttgt gccgccaaag gagtgatctg ccgacgggcc
     9301 cgcactcacc gatccgacac acttgcccgg tttcggcgga ttcatacgga tgccgactag
     9361 agttgcggac ctcccgacgc acgcctattg cccgtaaatc acaccttcac ccagcaccga
     9421 gtcccgcatg ggcggctccc ggggagcaca cgcccgcgca caccgaggcc cgcgccgccg
     9481 accgagaagt gttcgaacag gacaaggcgc cgcgaaccgg tggtgaccag ttgtccttcc
     9541 ggctcgtttc ccagcgggac gcagagcgac acccgtgagc gaggaaaccg cgcaatgcac
     9601 ctgtcagcca acgacccccc gcctcgggtc ctgcatctga cccagcccgt cgacggcggc
     9661 gtcgcccggg tcgtgaccga tctggcgcgg gcccagctcg cggccggact gcacgtcacc
     9721 gtggcctgcc cggacagtgc cctgagcacc gcgctggggg cgctcggcgc cgacgtacgc
     9781 gcctggcccg cgacccgtgc gccgggcccg acactcgttc gggaggtacg gctgctcacc
     9841 cggctggtgg aggacgtacg gcccgatctg gtgcacgcgc acagcgccaa ggccggtctg
     9901 gccgggcggc tggcgctgcg cgggcggatc ccgaccgtgt tccagccgca cgcctggtcg
     9961 ttcgaggcgg tcgacggggc cacggccgcc ttcgcactca actgggagcg gcaggcggcg
    10021 cgttgggcct cgcgggtggt gtgcgtcagc gaggcggagc gggtgaccgg ggtacgcgcc
    10081 gggatccgag gccggtacac ggtgatcccc aacggcatcg accccgagcg cttccacccc
    10141 gcccccgtcg gcaccgtgcg cgccggtctg ctgccggagg tcgagcccgg ggccccgctg
    10201 gtggtgtgcg tgggacggct gtgccgccag aagggccagg acgtcctgct gcgggcctgg
    10261 gccgcggtgg cggaacaggt gcccggggcc cggctggtgc tggtcggtga cggtcccgac
    10321 ggtggcgcgc tgcgcgccag ggcgccgcgc tcggtgctgt tcgcgggcgc cgtcaccgac
    10381 gccgccccct ggtaccaggc cgccgacctg gtcgtactgc cctcgcgctg ggagggcatg
    10441 gcgctcgccc cgctggaggc gatggcgtgc g
//
