LOCUS       NZ_CP104794            10405 bp    DNA     linear   CON 09-JUL-2024
DEFINITION  Streptomyces sp. FZ201 chromosome, complete genome.
ACCESSION   NZ_CP104794
VERSION     NZ_CP104794.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. FZ201
  ORGANISM  Streptomyces sp. FZ201
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8604396)
  AUTHORS   Zhang,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2022) College of Life Science and Technology,
            Jinan University, Guangzhou, Guangzhou, Guangdong 510632, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2021
            Assembly Method                   :: unicycler v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 235.1x
            Sequencing Technology             :: Illumina NovaSeq6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025402955.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 06:54:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,562
            CDSs (total)                      :: 7,471
            Genes (coding)                    :: 7,336
            CDSs (with protein)               :: 7,336
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 55 of 135
            Pseudo Genes (incomplete)         :: 92 of 135
            Pseudo Genes (internal stop)      :: 7 of 135
            Pseudo Genes (multiple problems)  :: 18 of 135
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:45:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1903355
            Orig. end                         :: 1913760
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Zhang Fei, College of Life
            Science and Technology.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    1128..1142
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    1550..1565
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            complement(1767..2246)
                     /locus_tag="N6J21_RS08385"
     CDS             complement(1767..2246)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310217.1"
                     /locus_tag="N6J21_RS08385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_261718021.1"
                     /transl_table=11
                     /translation="MLDQALTALAAAGGAAVVQAAGTDLWTGLRQAVAGWFGRGDAERE
                     QAELQRLDRTAAELRSAPADEAERLRIRAEAAWQARMEALLENLGEVERSRAAEELRTL
                     LAEQNARGGGPTTGSGGVAVGGDLDIRADGGSIAAGVIHGGAHIDRPPTPDPSQG"
     gene            2351..2887
                     /locus_tag="N6J21_RS08390"
     CDS             2351..2887
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002247.1"
                     /locus_tag="N6J21_RS08390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_261718022.1"
                     /transl_table=11
                     /translation="MTTDDRIPVALPDGRAVPPRLGDERAMLKGWLDFHRGTLEVKCAG
                     LNDTQVRVAAAEPSALTLLGLVQHMAEVERNWFQRAFGAVGDGTVFGDETGFALTDERG
                     LDEALGVWRREIARGRDVCAGRSLDDVGRIPGDGPFGGAEVSLRWVYLHMIEEYARHNG
                     HADILREHIDGVTGS"
     misc_feature    complement(2905..2919)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.33"
                     /tool="antismash"
     misc_feature    complement(2907..2920)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     misc_feature    complement(2959..2976)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.78"
                     /tool="antismash"
     misc_feature    3139..3151
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3176..3682
                     /gene="ectA"
                     /locus_tag="N6J21_RS08395"
     CDS             3176..3682
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661364.1"
                     /locus_tag="N6J21_RS08395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_093722461.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPTVADGAALWRIAKDSRTLDLNSSYSYLLWCRD
                     FAGTSAVARDEDGRPVGFVTGYIRPARPRTLLVWQVAVDDAHRGRGVAAALLDGLVARV
                     AGAYGVTGVETTITPGNTASERLFTSFAERHGAALEREVLFEAGLFPDGPHDSEVLYRI
                     GPLSH"
     gene            3710..4981
                     /gene="ectB"
                     /locus_tag="N6J21_RS08400"
     CDS             3710..4981
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 2.8e-73.
                     Score: 238.8. Matches aSDomain:
                     nrpspksdomains_N6J21_RS08400_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="N6J21_RS08400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093722462.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9e-77, bitscore:
                     257.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFQTLESEVRSYCRGWPTVFDRARGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTGAKRAFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFRPELDIWEPGEHNGTFRGNNPAFVTATAALQTYWADGSAMEKQTRARGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGLEFHAKDRADRIARRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDAGLSVLARAVRETI"
     aSDomain        3794..4807
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS08400_Aminotran_3.1"
                     /evalue="2.80E-73"
                     /label="N6J21_RS08400_Aminotran_3.1"
                     /locus_tag="N6J21_RS08400"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.8"
                     /tool="antismash"
                     /translation="FDRARGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTGAKRAFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFRPELDIWEPGEHNGTFRGNNPAFV
                     TATAALQTYWADGSAMEKQTRARGEQVEQALISITEENLADVKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="N6J21_RS08405"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="N6J21_RS08405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_093722463.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.4e-53, bitscore:
                     178.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHESGLTHTITPGTMYLLDGHERHTLRVKQDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5412..6296
                     /gene="thpD"
                     /locus_tag="N6J21_RS08410"
     CDS             5412..6296
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="N6J21_RS08410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_261718023.1"
                     /sec_met_domain="PhyH (E-value: 8.8e-40, bitscore: 136.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMTDLYPSRGATEVTLPRQDPVVWSAPGTPGPISGPELQAYERD
                     GFLPVEQLINDDEVAVYRRELERLVTDPEIRADERSIIEPKSKEIRSVFEVHRISEVFA
                     NLVRDERVVGRARQLLGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRTV
                     SVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEALTKMA
                     SEHGIRLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENKAVEPFAAPVRR
                     PEFIGARDFTPVR"
     gene            complement(6300..7301)
                     /locus_tag="N6J21_RS08415"
     CDS             complement(6300..7301)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661360.1"
                     /locus_tag="N6J21_RS08415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_261718024.1"
                     /transl_table=11
                     /translation="MTEHEDVRELLAAWAFGALDAADADRVRPHLAGCESCAAEAERLR
                     ETVRLLDGPPIGGLPARPATGLPPALLARRPTAPRTAAHAACYAAAVQALRALLPEAEG
                     RWATPVVHDWDVHATVAHLLAADEALAGRLGIPARVPETVLPQDMRWEEAWARRTADVI
                     AHEHARTPGETVASWAGQADALLATPEARDPELAARAELLMGLRLPVADHFAVRAFETW
                     IHTDDIGRALGLVVPPPPEPHLDQLVRLAVRVLGLALGPEATPVLFSVTDGEEWGLGSS
                     AEPVAAELTLDPVDFCLLMGGRTAPEEVPRGATGDAAAVRNVLEAAASLAWL"
     gene            complement(7342..>7470)
                     /locus_tag="N6J21_RS08420"
                     /pseudo=""
     CDS             complement(7342..>7470)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="N6J21_RS08420"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RNA polymerase subunit sigma"
                     /pseudo=""
                     /transl_table=11
                     /translation="YRQAAVELGIPEGTAKSRLRTALRSLAETLADPPDPGRARGA"
     gene            7482..8353
                     /locus_tag="N6J21_RS08425"
                     /pseudo=""
     CDS             7482..8353
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377534.1"
                     /locus_tag="N6J21_RS08425"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDQSVVGPGVPLTDAQWARIEPLLPDRTPKRGGRWRDHREVIDAI
                     AYKFQTGTQWVHLPEKYGNWRGVYNRLRMWAVDGTWERVFTTLVAQADADEDLNWAVSV
                     DSTIVRAHQHAAGARKRGPRPESRPTMPSAGPAAD"
     gene            complement(<8372..8786)
                     /locus_tag="N6J21_RS08430"
                     /pseudo=""
     CDS             complement(<8372..8786)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436208.1"
                     /locus_tag="N6J21_RS08430"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /pseudo=""
                     /transl_table=11
                     /translation="MESTGEVPSNPAADEELHRRLVYGDESALAEVYAAYLPLVRRIAV
                     RVTRTPGAAEDVAQEVFAQLWSRPYAYDPGRGTLRTWLSLLAHRRAVDWVRGEARHRKH
                     AHADDDALRTVPEPGPGSRTAARGGPPRLLRGPH"
     gene            complement(8827..9879)
                     /locus_tag="N6J21_RS08435"
     CDS             complement(8827..9879)
                     /NRPS_PKS="Domain: Aminotran_5 (16-344). E-value: 2e-26.
                     Score: 84.7. Matches aSDomain:
                     nrpspksdomains_N6J21_RS08435_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661358.1"
                     /locus_tag="N6J21_RS08435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_250747028.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3e-20, bitscore:
                     71.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPGNTYLNTASNGLLPARTVDAVQAAVRMRAEG
                     RPLGSLYDDVETVRAAFARIAGVPTERVALGTSVAEYGALIAAALPAGAEVLTAEADFA
                     SLVNPFHMRGDLKVRAVPLERLAESVRPETALVAVSAVQSADGRLADLAAVREAARAHG
                     ARTYVDASQSVGWLPLDAGAHDFVAAVGFKWVLGPHGNTFFVAPEDFGELTPLLAGWVA
                     GEVPWDSCYGPVQELAHSVRRFDVSPALFTYAGLRASLSLVEELGIDAIHAHDVGLADR
                     FRTGLASLGRTPVPSPGSAIVSVPGLGHRQPELSEAGIEVSNRAGNLRAAFHLYNTAAD
                     VDRLLDVLSS"
     aSDomain        complement(8848..9831)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N6J21_RS08435_Aminotran_5.1"
                     /evalue="2.00E-26"
                     /label="N6J21_RS08435_Aminotran_5.1"
                     /locus_tag="N6J21_RS08435"
                     /protein_end="344"
                     /protein_start="16"
                     /score="84.7"
                     /tool="antismash"
                     /translation="TYLNTASNGLLPARTVDAVQAAVRMRAEGRPLGSLYDDVETVRAA
                     FARIAGVPTERVALGTSVAEYGALIAAALPAGAEVLTAEADFASLVNPFHMRGDLKVRA
                     VPLERLAESVRPETALVAVSAVQSADGRLADLAAVREAARAHGARTYVDASQSVGWLPL
                     DAGAHDFVAAVGFKWVLGPHGNTFFVAPEDFGELTPLLAGWVAGEVPWDSCYGPVQELA
                     HSVRRFDVSPALFTYAGLRASLSLVEELGIDAIHAHDVGLADRFRTGLASLGRTPVPSP
                     GSAIVSVPGLGHRQPELSEAGIEVSNRAGNLRAAFHLYNTAADVDRL"
ORIGIN
        1 gcgcagccac gcctccgctt cgtcgtcccc gcggaggcgc agcaagtgcc cgtgcacgta
       61 catcgcctcg gtgtctcccg ccagcgccgc ccgccgccac cacggctccg cctccgcctc
      121 gtcgtcccgt tggtgcagga gcgtcgccag cgtggtcatc gccttgacgt agccgtcctc
      181 cgccgccctc cgcagccacc tctcggcctc ctggtcctcg cctttgctgc ggtagaacgc
      241 gaagaggctg taggaggcga gagacagtcc gccctccgct gcgctcagcc accatctctc
      301 cgcctcggcg tcctcgttcc gcattctcag ggcgccggcg aggttgagtt gggcgaccgg
      361 gttccccgcc tccgcgagcg ggcgcagcag cggttctacc tcgtgcgggt ccccgcgctg
      421 gaacagttgc gccgacgtgt agttgatcac cgcttccgcg tcgcccgact ccgccagcgg
      481 ccgcagggcc cgctcggcga tggcccaccg ccggctggac atcgccgtcc gggcgatgag
      541 cggccgggtg aggtcgtcgg cgtactccag cgccgcgtgc cacatcgagt cgggcggatc
      601 cggcaccccg ggatcgcggg ccacggagtc gacgaggtag tcgaaggggc gcttggtccc
      661 ttcccgctcg ccctccatca gcaggccggc cacgccgtag cggatccggg aggcccaggc
      721 cacggcgtcg gcgtaggact cggggcgcag ggccgggccg ccgagggcgc ggaggtagcc
      781 ctcgtgcacc tcccgcagca gcgcttccgg cacatcgccg atcaggccga tgtgggtcag
      841 gtccacggct gccgcgacca gtgcggcccc gcgtgggtgg cctcctctgg tggtcgctcg
      901 ttgcgctctg cgccactccg cgagcagttc gggtccggcg gcgaggtact cggcgatgcc
      961 gtgggtgccg tggcgtgcgt acgcctcagt cagacggctg tcgtcgactt cggcgacccg
     1021 ggccagttcg ctgtcgctcc acagccgtcg tacgacgatg ggctccacca tgttgaggac
     1081 gcgcagtcca ctctcggcgg ccaggcggct cgggccgatc tcaccggcga cgccgtcggc
     1141 catgtcccgc agggtgtcgt agcgctcgtc ccggagggtc gccagcacca ccagaccggc
     1201 agcggtcagg gacgtcagca gggacggttc caggcggccg ggtccgaggt ggtgctccag
     1261 atcgtcgagc cagagcacgt gccgctcgta ccccggttcg ccgggtgccc gcagcaacgg
     1321 ccgcaggtcg gcgcccgagg gcggggcgag cagaagacag ccggggagca cggcccgtag
     1381 cgcctcgatg gccgtgcggg tcttgccggc ggtcgagtcc ccggtgagca gtacgaaccc
     1441 gccggtctcg gcggcggctg ccagcgccgc ccgcacctgg tcgtccacgt cgcgcggcac
     1501 gtacggcggc agtgccgatc cgtcgtcctc gccaagacgg gcgcgatgca cgccgaggcc
     1561 gagcaggtcg gtttcgccga ccggttgcca ggaacccaga gcggtcggtt ccgcctcacc
     1621 cgccctgtag tggtggtgga tcccaccggc gacgtagtgc gcggcgacac cgccgtgtgt
     1681 ggccaggagg cgggccgact cggccgcggc cgggggcgtg tccgcacgga cagcgggcag
     1741 ggcttggcgc gcggtcgccg gtccggtcag ccctgggacg gatccggcgt cggagggcgg
     1801 tcgatgtgcg cgcctccgtg gatgactccc gccgcgatcg agccgccgtc ggcgcggatg
     1861 tccaggtcgc cgccgaccgc cacgccgccc gagccggtcg tgggcccgcc gccccgggcg
     1921 ttctgttccg cgagcagggt gcgcaactcc tcggcggcgc gactccgttc cacctcgccc
     1981 agattctcca gcagcgcctc catacgggcc tgccaggccg cttccgcgcg gatacggagc
     2041 cgctccgcct cgtcggcggg cgcggaccgg agttcggcgg cggtccggtc caggcgctgg
     2101 agttccgcct gctcgcgctc cgcgtcaccg cggccgaacc agcccgccac cgcctgccgc
     2161 aggccggtcc acaggtccgt cccggccgcc tgcaccacag cggctccgcc ggccgcggcc
     2221 aacgctgtca acgcctgatc cagcatcccc gcccgtcctc cccgcaccac tggtccacca
     2281 gcaacttacg gccacctccc ggcccttcgc agcgataccg gaaaatcgcg acatgggcca
     2341 gggtgggacc atgaccactg atgaccggat ccctgtcgcc cttcccgacg gccgcgccgt
     2401 gccgccccgg ctcggggacg aacgggccat gctcaaaggc tggttggact ttcaccgggg
     2461 cacgctggag gtgaagtgcg ccgggctgaa cgacacccag gtgcgggtcg ctgccgccga
     2521 gccctcggcg ctcactctgc tgggactcgt gcagcacatg gccgaggtgg aacggaactg
     2581 gttccagcgg gcgttcgggg ccgtcggcga cgggaccgtg ttcggggacg agaccgggtt
     2641 cgcgctcacg gacgagcggg ggctcgacga ggcgctcggt gtgtggcggc gggagatcgc
     2701 gcgggggcgg gacgtgtgtg ccgggcggtc gctcgacgac gtcgggcgca tcccggggga
     2761 cgggcccttc ggtggggccg aggtcagtct gcggtgggtg tatctgcaca tgatcgagga
     2821 gtacgcgcgc cacaacgggc acgccgacat cctgcgcgag catatcgacg gagtgaccgg
     2881 cagctgaatt cacggcaatt cttgaattcc cttaaaagat gcagcttccc gttctcttct
     2941 gcccggtttc cgacggcgta aacacaaaga tttcatgaac cccaagcacg cctaattcgg
     3001 gcgcgattca gcgagattac acgactgtga cgcgttcaac atcgcgcccg gtttgccgct
     3061 tattcacggt gcataaccgg gcaagtccga cctcgcgcga ataacacatc accgccggcc
     3121 gcgtaacccc tccccgccat gcaattttga attctgctcg gtaagttcaa ttcgcatgac
     3181 tgccgcacaa gcagacctgc aaatcgaccg gccgacggtg gccgacggcg ccgcgctgtg
     3241 gcgtatcgcc aaggactcca ggacgctcga tctgaactcg tcctacagct atctgctgtg
     3301 gtgccgggac ttcgccggca cctcggccgt cgcccgcgac gaggacgggc ggccggtcgg
     3361 cttcgtcacc gggtacatcc ggcccgcccg cccgcggacc ctgctggtct ggcaggtcgc
     3421 ggtggacgac gcccaccgtg gccgcggcgt cgccgccgcg ctgctggacg ggctcgtcgc
     3481 acgggtcgcc ggcgcgtacg gcgtcaccgg cgtggagacc accatcacgc ccggaaacac
     3541 cgcctccgag cggctgttca cctccttcgc cgagcggcac ggcgccgctc tggagcgcga
     3601 ggtgctgttc gaggcgggcc tgttccccga cgggccgcac gactcggaag tcctgtaccg
     3661 catcggtccc ctctcccact gacctcccca caacgaggag cgattcgtcg tgaccatcac
     3721 ccagcccgac ctcagcgtct tccagaccct ggagtccgag gtacgcagct actgccgcgg
     3781 ctggcccacc gtcttcgacc gtgcgcgggg cagccgcatg tacgacgagg acggccacga
     3841 gtacctggac ttcttcgccg gggccggctc actgaactac gggcacaaca accccgtcct
     3901 gaaacgggcg ttgatcgact acctggagcg ggacggcgtc acgcacgggc tcgacatgtc
     3961 gaccggcgcc aagcgggcgt tcctgcagac cttccaggac ctggtgcttc ggccgcggga
     4021 tctgccgtac aaggtcatgt tcccggggcc gacgggcacg aatgccgtcg agtccgcgct
     4081 gaagctggcg cggaaggtga aggggcgcga ggccatcgtg tccttcacca atgccttcca
     4141 cgggatgtcg ctggggtccc tcgccgtcac cggtaacgcc ttcaagcggg ccggggcggg
     4201 gatcccgctg gtgcacggca cgcccatgcc gttcgacaac tacttcgacg gcaccgtccc
     4261 ggacttcctg tggttcgagc ggctgctgga ggaccagggg tccgggctga acaagcccgc
     4321 cgcggtgatc gtggagaccg tgcagggcga gggcggcatc aacgtcgccc ggcccgaatg
     4381 gctgcgcgcg ctcaaggagt tgtgcgagcg gcaggacatg ctgctgatcg tcgacgacat
     4441 ccagatgggc tgcggccgta ccggcgcctt cttctccttc gaggaggcgg gcatcacgcc
     4501 ggacatcgtc accgtgtcca agtccatcag cggctacgga ctgcccatgt ccctgtgtct
     4561 gttccgaccg gagctggaca tctgggagcc gggcgagcac aacggcacgt tccgcggcaa
     4621 caaccccgcc ttcgtcaccg cgaccgccgc gctgcagacg tactgggccg acggcagcgc
     4681 gatggagaag cagacccggg cccgcgggga gcaggtcgag caggcgctga tctccatcac
     4741 ggaggagaac ctcgccgatg tgaaggagta ccggggccgc gggctggtgt gggggctgga
     4801 gttccacgcc aaggaccggg ccgaccggat cgcgcggcgc gccttcgaac tcgggctgct
     4861 gatcgagacg tccggccccg agagcgaggt cgtgaagctg ctgcccgcgc tgaccatcac
     4921 ccccgaggaa ctcgacgcgg gcctgagcgt gctcgcccgt gccgtccgag aaaccatctg
     4981 atcaggaggc gtccgaaact gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgaa ggcggcgtcc ggcacctggg agagcaaacg catcgtcctc gccaaggagc
     5101 gggtcgggtt ctccctgcac gagacgattc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcg gtcgtctgtg tcgagggcga ggccgaactg accgatcacg
     5221 agtccgggct gacccacacg atcacgcccg ggaccatgta cctgctggac ggccacgaga
     5281 ggcacacgct gcgggtgaag caggacttcc gctgcatctg tgtcttcaac ccgccggtga
     5341 ccggccgcga ggaccacgac gagaacggcg tgtatccgct gctcaccgaa cccgaggagg
     5401 tgtgacgcat catgacgatg accgatctgt accccagccg aggcgccacc gaggtgacgc
     5461 tcccgcgcca ggacccggtg gtgtggtccg cgcccggcac gccgggcccg atctccggcc
     5521 cggagctcca ggcctacgag cgtgacggct tcctgcctgt cgagcagctg atcaacgacg
     5581 acgaagtcgc cgtgtaccgg cgtgagttgg agcggctggt caccgacccg gagatccgtg
     5641 ccgacgagcg ctcgatcatc gagccgaagt ccaaggagat ccgctcggtg ttcgaagtgc
     5701 accggatcag cgaggtgttc gcgaacctgg tgcgcgacga gcgggtcgtc gggcgcgccc
     5761 ggcagctgct cggctccgac gtgtacgtcc accagtcccg gatcaacgtc aagccggggt
     5821 tcggcgccag cggcttctac tggcactccg acttcgagac ctggcacgcc gaggacggcc
     5881 tgcccaacat gcgcacggtg tccgtctcga tcgcgctgac cgagaactac gacaccaacg
     5941 gcgggctcat gatcatgccg ggctcgcaca agacgttcct gggctgcgcg ggcgccaccc
     6001 cgaaggacaa ctacaagaag tccctccaga tgcaggacgc gggcaccccg tccgacgagg
     6061 ccctcaccaa gatggcctcc gagcacggca tccgcctgtt caccggcagg gcgggctcgg
     6121 cgacctggtt cgactgcaac tgcatgcacg gctcgggcga caacatcacc ccgttcccgc
     6181 gcagcaatgt cttcatcgtc ttcaacagcg tggagaacaa ggcggtcgag ccgttcgcgg
     6241 ccccggtccg ccgcccggag ttcatcgggg cgcgggactt caccccggtc cggtagcccc
     6301 tacagccacg ccagtgacgc ggccgcctcc agtacgttcc ggacggcggc cgcgtcgccc
     6361 gtggcgccgc gcgggacctc ctcgggggcc gttcggccgc ccatgaggag gcagaagtcg
     6421 accgggtcca gggtgagttc ggcggcgacg ggctcggccg acgagcccag gccccactcc
     6481 tcgccgtcgg tgacggagaa gaggaccggg gtcgcttcgg ggcccagggc gaggcccagc
     6541 acccggaccg ccaggcggac cagctggtcc aggtggggct cggggggcgg tgggaccacc
     6601 aggcccaggg cgcggccgat gtcgtccgtg tggatccagg tctcgaaggc ccggaccgcg
     6661 aagtggtcgg cgaccgggag acgcaggccc atcaacagct cggcgcgcgc ggccagttcg
     6721 gggtcgcggg cctcaggggt ggccagcagc gcgtccgcct gccctgccca ggacgcgacg
     6781 gtctccccgg gggtacgggc gtgctcgtgg gcgatcacgt cggcggtgcg ccgggcccag
     6841 gcctcctccc agcgcatgtc ctgcgggagc acggtctccg ggacccgcgc ggggatgccg
     6901 aggcggccgg ccagtgcctc gtcggcggcc agcaggtggg cgacggtcgc gtggacgtcc
     6961 cagtcgtgga cgaccggcgt ggcccagcgg ccctccgcct cgggcagcag ggcccgtagc
     7021 gcctggacgg cggcggcgta gcaggcggcg tgggcggcgg tgcggggcgc ggtggggcgg
     7081 cgggccagca gggcgggtgg aagtccggtg gcggggcggg cgggcagtcc gccgatgggc
     7141 gggccgtcca gcagccgtac cgtctcgcgc agccgttccg cctccgccgc gcagctctcg
     7201 cagcccgcca gatgcggccg gacccggtcg gcgtcggccg cgtccagggc gccgaaggcc
     7261 caggcggcca gcagttcgcg tacgtcctcg tgctcggtca ccgaacgcct ccttcccgtg
     7321 gagcacggac ccctactgcc atcatgcgcc tcttgcccgc ccggggtcgg gtgggtcggc
     7381 cagcgtttcg gccagcgagc gcagcgcggt gcgcagcctg gacttggcgg tgccctcggg
     7441 aatgcccagc tcaacggcgg cctgccggta ggcagtttcg tttggatcag tcggtcgttg
     7501 gtccgggtgt gccgttgact gatgcgcagt gggcgcggat tgagccgttg ctcccggacc
     7561 ggactccgaa gcggggtggc cgctggcggg accatcgtga ggtgatcgac gcgatcgcct
     7621 acaagttcca gaccggcacg cagtgggttc acctgccgga gaagtacggc aactggcgag
     7681 gggtctacaa ccgcctccgg atgtgggccg tcgacggcac ctgggagcga gtctttacca
     7741 ccctggtcgc ccaggctgac gcggacgagg acctcaactg ggccgtctcg gtcgactcca
     7801 ccatcgtgcg ggcccaccag cacgcggccg gagcccgcaa aagggggccc cggccggaga
     7861 gccggccgac catgccatcg gccggtcccg cggcggactg accacgaaga tccacctcgc
     7921 tgccgatgcc cgctgccggc ccctggcgtt cgttctcacc gcaggccagg ccggtgacgc
     7981 acccgcgttc accgacgtca tggcccgcct gcgcgttccc cgccggcgag gacggccccg
     8041 caccaggcca gacctggtgc tggccgacaa ggcgtactcc tcccgcgcga tccgcgagca
     8101 cctgcgcaag cgcggcatcc gggcagtgat cccggtcccc gcagaccagc gcagacaccg
     8161 gctacgtcgg ggcagccggg gcggcagacc accggccttc gaccgcgaga cctacaagca
     8221 gcgcaacacc gtcgagcggt gcatcaaccg cctcaagcaa tggcgaggca tcgccacccg
     8281 ctacgagaag accgccacca tctacctggc cggactccac atcgcaggca tcttcctttg
     8341 gtccgcccgg tgatccaaac gaaactgcct aggtgcggcc cgcgaagtag gcgaggtgga
     8401 ccacctctcg ctgcggttcg ggatccgggt ccgggttccg gaaccgtgcg cagggcgtcg
     8461 tcgtcggcgt gggcgtgctt gcggtgccgg gcctcgccgc gcacccagtc cacggccctc
     8521 cggtgggcga gcagggacag ccaggtgcgc agcgtgccgc ggcccgggtc gtaggcgtac
     8581 ggcctgctcc acagctgggc gaagacctcc tgtgccacgt cctcggcggc gcccggggta
     8641 cgggtcaccc gtaccgcgat ccggcgcacc agcggcaggt acgccgcgta cacctcggcc
     8701 agcgcggact cgtcgccgta caccagccgc cggtgcagct cctcgtcggc ggccgggttc
     8761 gacggcacct cgcccgtgga ctccactccc ctttcctagc gtcccggccg gggccgcgcc
     8821 agtggttcag gacgacagga cgtccagcag ccggtccacg tcggcggccg tgttgtacag
     8881 gtggaacgcg gcacgcaggt tgcccgcgcg gttggacacc tcgatgcccg cctcgctcag
     8941 ctcgggctgg cggtggccga ggccggggac ggagacgatg gccgagcccg gggacgggac
     9001 cggggtgcgg ccgagggagg ccaggccggt gcggaagcgg tcggcgaggc ccacgtcgtg
     9061 ggcgtggatc gcgtcgatgc ccagctcctc gacgagggag agcgaggcgc gcaggcccgc
     9121 gtacgtgaac agggcggggc tgacgtcgaa ccgccgtacg gagtgggcga gttcctggac
     9181 cgggccgtag cagctgtccc aggggacctc gcccgcgacc cagcccgcca gcagcggggt
     9241 cagctcgccg aagtcctcgg gcgcgacgaa gaaggtgttg ccgtgcggcc ccaggaccca
     9301 cttgaagccg acggcggcga cgaagtcgtg ggcgccggcg tccagcggca gccagcccac
     9361 cgactgggag gcgtcgacgt aggtacgggc cccgtgggcg cgggccgcct cgcgcaccgc
     9421 cgccagatcc gcgagccgcc cgtcggcgga ctgcacggcg ctgaccgcga ccagcgcggt
     9481 ctccgggcgc accgactcgg cgagccgctc cagcggcacg gcccgcacct tcaggtcgcc
     9541 gcgcatgtgg aaggggttga ccagggaggc gaagtcggcc tcggcagtga ggacttcggc
     9601 tcctgcgggc agtgcggcgg cgatcagcgc gccgtactcg gcgaccgagg tgccgagcgc
     9661 gacccgctcg gtgggcaccc cggcgatccg ggcgaaggcg gcccggaccg tctccacgtc
     9721 gtcgtagagg gacccgagcg gccgtccctc ggcccgcatc cggacggcgg cctgtacggc
     9781 gtcgacggtg cgggccggca ggaggccgtt gctcgcggtg ttgaggtagg tgttccccgg
     9841 ggcgaactcg gcacggacga ggctctcgaa ggtctccatg ggaccactct ggaggcccgg
     9901 gaactccccg tccattgcga atttttacgt ggtttcgcta agcagtcctt atggatccgc
     9961 gccgacctgc gactttgccg gtcccgaggg agcccgcccc gacctgcggc tttcagtgct
    10021 gcggcaccgc gcacccgtcg gggccgcacg cctcgccctc gccctcgccc tcggcccggt
    10081 cgatgagcgt gagcggggag cgctcgtccc atgccgtggt gaggacctgc gtgaacacgt
    10141 cggtgggctg ggcgccggag acgccgtact tgcggtcgac gacgaagaac ggcacaccgt
    10201 tcgcgccgag ctgcgccgcc tcgcgctcgt cggcgcggac gtcgtcggcg taggcggtgg
    10261 ggtcggcgag caccgcgcgg gcggcgttct cgtccaggcc cgcgccgacg gcgagcttca
    10321 ccagccgctc gtcgtcgccg aagacggact cctcctcggc gaagttcgcc tcgtacagcg
    10381 cctggacgag ttcgccctgg cggcc
//
