LOCUS       NZ_CP104098            10936 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces noursei strain IFO14147 chromosome, complete genome.
ACCESSION   NZ_CP104098
VERSION     NZ_CP104098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Transcriptome and Metabolome Analysis Revealing the Improved
            epsilon-Poly-l-Lysine Production Induced by a Microbial Call from
            Botrytis cinerea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) College of life science, Huaibei Normal
            University, Dongshan road 100#, Huaibei, Anhui 235000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PGCGAP v. 1.0.34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025163575.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 12:09:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,195
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,883
            CDSs (with protein)               :: 7,883
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 93 of 218
            Pseudo Genes (incomplete)         :: 149 of 218
            Pseudo Genes (internal stop)      :: 11 of 218
            Pseudo Genes (multiple problems)  :: 33 of 218
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8331521
            Orig. end                         :: 8342457
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="30"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(869..1252)
                     /locus_tag="N1H47_RS36915"
                     /old_locus_tag="N1H47_36910"
     CDS             complement(869..1252)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379595.1"
                     /locus_tag="N1H47_RS36915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36910"
                     /product="AQJ64_40280 family protein"
                     /protein_id="WP_037634554.1"
                     /transl_table=11
                     /translation="MTNLHTVVTWIDARERLPRSGVPVAAAITGRYPADGTGESDAGSG
                     EEFWLVRPMYFTTLHRGEDGVEHRDCFVDSDGIVRLPHGLASAETVTHWAELPVLPGQQ
                     THIALGGDVQPALRNAWDARPTT"
     gene            complement(1457..1864)
                     /locus_tag="N1H47_RS36920"
                     /old_locus_tag="N1H47_36915"
     CDS             complement(1457..1864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573067.1"
                     /locus_tag="N1H47_RS36920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36915"
                     /product="Lsr2 family protein"
                     /protein_id="WP_016573067.1"
                     /transl_table=11
                     /translation="MAVKETIQVDESQKNEPGVQEVITPVPVGSEVIKKATYWRSILQD
                     DLDPQATDGVTTVKLAVPALVEEEYETGETNEDGTAKLGVRQIRDTQWYEIDLSEANVA
                     ALQEAVKPFTDVARTIEAPTVKPARKKRTTK"
     misc_feature    complement(2060..2075)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.35"
                     /tool="antismash"
     gene            complement(2202..2843)
                     /locus_tag="N1H47_RS36925"
                     /old_locus_tag="N1H47_36920"
     CDS             complement(2202..2843)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573068.1"
                     /locus_tag="N1H47_RS36925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36920"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_016573068.1"
                     /transl_table=11
                     /translation="MDSQEIVDRSHSLDGRPELLSAFYGDWAETYESDASNMGYEGPLR
                     MADFYLSLAPRMLPAPEETTALDAGCGTGLVGPVFKKLGIGSLDGLDLNHEMVTQAAGT
                     GAYRLLTSGTDLNLGLPEFAPDTYDLTLSCGVFTTGHVAPNALDTLLRLTRPNGLVLLT
                     TSANYLSVTDFHGFLESRAAQGRLTVIECLADHHYLTDERAHYWAISPTA"
     gene            complement(2938..3753)
                     /locus_tag="N1H47_RS36930"
                     /old_locus_tag="N1H47_36925"
     CDS             complement(2938..3753)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573069.1"
                     /locus_tag="N1H47_RS36930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36925"
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="WP_016573069.1"
                     /transl_table=11
                     /translation="MDKPIIAYLSDIGNHDEAHALGKGLIKTIAPGAEIVDITHQVTPF
                     DVREGGLYLQDVPASFPANTVIAAYVYPETGTSTRTVVVRNEKGQLLVAPNNGLLTWAL
                     KAVPAVEAWEVTSPDVMNQPVTPTWYGKDVVVACGAHLAAGVAPSAVGPKIDVAKLVTL
                     PTTPAVQLGDGSVRGEVVRIDKAFGNVWTNISLDALSGGGALDGKTLQVTAEGLSVEIP
                     YYATFGEVPIGEPLVYNNSRGKVALGLNQGSFLERYGVAAGDTVTIGLV"
     misc_feature    complement(4173..4186)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.27"
                     /tool="antismash"
     gene            4176..4928
                     /locus_tag="N1H47_RS36935"
                     /old_locus_tag="N1H47_36930"
     CDS             4176..4928
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-158). E-value: 8.8e-15. Score:
                     47.0. Matches aSDomain:
                     nrpspksdomains_N1H47_RS36935_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433623.1"
                     /locus_tag="N1H47_RS36935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36930"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_037634555.1"
                     /sec_met_domain="adh_short (E-value: 5.4e-35, bitscore:
                     119.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVRETGRVAVVTGGTKGIGLAFTRKLVKLGYRVVALYRHDPEAA
                     RAAAAELGDACTVLQADVSDSAEVNRLADGVLAHHGVPHVLVNNAGRNIDRPFAEMSDD
                     AWHTVLNTNLSGPFYVARAFAPAMREAGSGVIVNIAATTGIRPRFNGINYCASKAGLLH
                     LTKCLALELAPHVRVNALIPGMIDTEDMQMRVRTAAPEHRASMLTSIPQERIGTVEEMA
                     AALEFLVGDGAEYVTGQKLIVDGGQFMW"
     aSDomain        4200..4649
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS36935_PKS_KR.1"
                     /evalue="8.80E-15"
                     /label="N1H47_RS36935_PKS_KR.1"
                     /locus_tag="N1H47_RS36935"
                     /protein_end="158"
                     /protein_start="8"
                     /score="47.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGGTKGIGLAFTRKLVKLGYRVVALYRHDPEAARAAAAELG
                     DACTVLQADVSDSAEVNRLADGVLAHHGVPHVLVNNAGRNIDRPFAEMSDDAWHTVLNT
                     NLSGPFYVARAFAPAMREAGSGVIVNIAATTGIRPRFNGINYCASK"
     CDS_motif       4206..4277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N1H47_RS36935_0001"
                     /evalue="8.50E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="N1H47_RS36935"
                     /protein_end="34"
                     /protein_start="10"
                     /score="18.4"
                     /tool="antismash"
                     /translation="VVTGGTKGIGLAFTRKLVKLGYRV"
     gene            complement(5001..5936)
                     /locus_tag="N1H47_RS36940"
                     /old_locus_tag="N1H47_36935"
     CDS             complement(5001..5936)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="N1H47_RS36940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36935"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_016573071.1"
                     /sec_met_domain="AfsA (E-value: 6e-19, bitscore: 66.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAPLAVPVASLPEAASDLGPLVRKRVSDEVLITDWRTVSGTTHH
                     VTAHWPQHHSFYTPRSGRYSSLLLVETVRQALALLSNAAYEVPLEYRLGWDYCSCTLTP
                     AMLDTPPDGHGAEVELTITHALPAARRKGGSVRLEAQITAVRDGQLLGAAHLRYTCHSP
                     AIYERLRGQYADARAATSHAPLPGTPVPPAHVGRSSAKDVVLCPTAAPRRWQLRVDTDH
                     PVLFDHAHDHLPGMVLLEAAGQAAHCLAGPDPTTPVTLRTRFTRYTELDAPCWIEATPL
                     EWPQPDLARTTITGTQNGQVTFTTQVITRR"
     misc_feature    complement(5733..5735)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6067..6690
                     /locus_tag="N1H47_RS36945"
                     /old_locus_tag="N1H47_36940"
     CDS             6067..6690
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573072.1"
                     /locus_tag="N1H47_RS36945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36940"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_016573072.1"
                     /transl_table=11
                     /translation="MSRQRQDRALQTRAAIVRAAAEVFAEQGYAGASVTKITERAGLTM
                     GAMYFHFKNKAALAREIVVGQPDRVLPRHESVGLQRAVDITLTWAHQIREDPVLLAGAR
                     LVMEQEEFIGQEQNSHQQWAQIFEQELAEAQKRGELTGTCDESAVARLIVNACTGAQMH
                     AHMLTGYRDLPIRVVEMWSCLLPSVATPDAAASIDLNTERGRAI"
     gene            6814..7701
                     /locus_tag="N1H47_RS36950"
                     /old_locus_tag="N1H47_36945"
     CDS             6814..7701
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (48-215). E-value: 9.8e-15.
                     Score: 46.9. Matches aSDomain:
                     nrpspksdomains_N1H47_RS36950_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489554.1"
                     /locus_tag="N1H47_RS36950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36945"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_078486931.1"
                     /sec_met_domain="adh_short (E-value: 2.3e-35, bitscore:
                     121.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 9e-31, bitscore:
                     106.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPTIPAQATGTAPRQQSAGQGRRASRGRPAVSRHREGDRGRGTL
                     NRKTIMITGASSGIGAAAAHCFAEEGASVVLMARREDRLRALAGEIEETGGQVAVVTGD
                     VTNQADVDRAVDVAVAEFGTLDAAFNNAGQGTLGAPLHETEQAVFDRIMDVNVRGVWNC
                     LRAQIRTMLTQGTGGAIVNTSSTAGLFATGAVAPYVAAKHAVLGLTKTAAAEYGDHGIR
                     VNALVVGATRTDMVTSVLQEVDGLREADLVARAIQRRLADPVEVARAAAWLCSDQASFI
                     TGGVVPVDGGWSAV"
     CDS_motif       6958..7053
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N1H47_RS36950_0001"
                     /evalue="2.50E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="N1H47_RS36950"
                     /protein_end="80"
                     /protein_start="48"
                     /score="10.5"
                     /tool="antismash"
                     /translation="TIMITGASSGIGAAAAHCFAEEGASVVLMARR"
     aSDomain        6958..7458
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS36950_PKS_KR.1"
                     /evalue="9.80E-15"
                     /label="N1H47_RS36950_PKS_KR.1"
                     /locus_tag="N1H47_RS36950"
                     /protein_end="215"
                     /protein_start="48"
                     /score="46.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TIMITGASSGIGAAAAHCFAEEGASVVLMARREDRLRALAGEIEE
                     TGGQVAVVTGDVTNQADVDRAVDVAVAEFGTLDAAFNNAGQGTLGAPLHETEQAVFDRI
                     MDVNVRGVWNCLRAQIRTMLTQGTGGAIVNTSSTAGLFATGAVAPYVAAKHAVLGLTKT
                     AAAE"
     gene            complement(7736..9187)
                     /gene="ahcY"
                     /locus_tag="N1H47_RS36955"
                     /old_locus_tag="N1H47_36950"
     CDS             complement(7736..9187)
                     /EC_number="3.13.2.1"
                     /GO_function="GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ahcY"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105956.1"
                     /locus_tag="N1H47_RS36955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36950"
                     /product="adenosylhomocysteinase"
                     /protein_id="WP_038524331.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 2.2e-09, bitscore:
                     35.8, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPADTTRFKVADLSLAAFGRKEITLAEHEMPGLMAIRKEYAEQQP
                     LKGARITGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQDHAAAAIAVGPNGTPEAP
                     QGVPVFAWKGESLEEYWWCTEQALTWPGTSTGGPNMILDDGGDATLLVHKGVEYEKAGK
                     APAVETAENEEHHAILELLNRTLADSPQKWTRLASEIRGVTEETTTGVHRLYEMQRDGV
                     LLFPAINVNDAVTKSKFDNKYGCRHSLIDGINRATDVLIGGKTAVVCGYGDVGKGCAES
                     LRGQGARVIITEIDPICALQAAMDGYQVATLDEVVETADIFVTTTGNKDIILASDMARM
                     KHQAIVGNIGHFDNEIDMAGLAKLPGIVKDEVKPQVHTWTHPDGKVLIVLSEGRLLNLG
                     NATGHPSFVMSNSFADQTLAQLELFTKPEQYPTDVYVLPKHLDEKVARLHLDALGVKLT
                     TLRKEQADYIGVKVEGPYKPDHYRY"
     gene            complement(9177..10067)
                     /gene="metF"
                     /locus_tag="N1H47_RS36960"
                     /old_locus_tag="N1H47_36955"
     CDS             complement(9177..10067)
                     /EC_number="1.5.1.20"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="metF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573702.1"
                     /locus_tag="N1H47_RS36960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_36955"
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="WP_016573702.1"
                     /transl_table=11
                     /translation="MSTLRADVTGPAPSFSFEFFPPRTEAGTRTLWRTIRRVEALAPTF
                     VSVTYGAGGSSRDHTVALTERIAADTTLRPVAHLTAVGHSVAELRHIIGRYSDAGVRDV
                     LALRGDPPGDPDGPWTPHPAGLTHAHELVSLVKDTGSFSVGVAAFPEGHPRSSDPDTDV
                     RHFIGKCRAGADYAITQMFFHVEDYLRLRDRVAAAGCDTPIIPEIMPATDVRQIRRFAQ
                     LSHAAFPEELAHRLEAARDSPADGHRIGVDHATRMALRLLDEGAPGLHYITLNRSSAAL
                     DIHGHVCRSRSLHAC"
     misc_feature    10507..10521
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 gaacccgacc gacgtcctgc tgcggcaagg ggcccacgcc gtgcgcatgc ccgaccggca
       61 gcccccgttc gtgcccggca tggacgccgc cggggtgatc gaccaactcg gagccgggac
      121 cgcaggccgt ttcgccctgg gccaacgcgt cgtcgccatg gtgctgttca ccagcgcgcg
      181 cggcggagcc tacgccgagc acatcgtggt gcccgccgcc tcggtggtgc cggtacccca
      241 gggagtggac ttccccgcgg cgtccaccct gctgatgaac gccatgaccg cacgcctggc
      301 actggacgca ctggccgtgc cgcccggcgg caccatcgcc gtgaccggtg cggccggtgc
      361 cgtcggcggg tacgcaatcg aactggccaa ggccgacggc ctgaccgtca tcgcggacgc
      421 cgccccgcac gacaccgacc gggtccgcgg cttcggcgcc gaccacgtcg tggcgcgcgg
      481 caccgaggtc gccgcccgca tccgcgcgct cgcaccggac ggcgttcccg gcctcgtgga
      541 cgcatcgatg cagaccaccg agatcacgtc ggcggtcgcc gacggcggga ccctggccga
      601 gttgcggggc tggtccggtc cggccgaacg cggcatccgg gtgcgtccgg tgatggtcac
      661 ggacggcatc acggacaccg cccggctgga cgcgctgcgc cgccaggccg aggagggcgt
      721 cctgacgctg cacgtcgccg acgtgctgcc cgccgaggag gcggccgagg cccaccgccg
      781 cctggaagca ggcggcctgc gcggccgctt ggtgctggac ttctcctagc ggacccgcac
      841 gcgacgtcaa cgggtctggg ggcgtcggtc aggtggtggg gcgggcgtcc caggcgttgc
      901 gcagggccgg ctgcacgtcc cctcccagtg cgatgtgcgt ctgctggccg ggcagcacag
      961 gcagttcggc ccagtgcgtc actgtttcgg cactggcgag gccgtggggc agacggacga
     1021 ttccgtcgga gtcgacgaag cagtccctgt gctccacccc gtcctcgccc cggtgcagcg
     1081 tggtgaagta catcggcctc accagccaga attcctcccc cgatccggcg tcggactcgc
     1141 cggtgccgtc cgccgggtac cggcccgtga tcgccgctgc caccggtaca ccgctccggg
     1201 gcagcctctc gcgtgcgtcg atccatgtca cgacggtatg gagattcgtc atcctgctcc
     1261 tcaccccaac actcgggaaa gcgcctcttg tccggggccg gcgcgtcggc gggggcgccg
     1321 gcccgacccc gacgtccgga aggaccgcgc ggcgcccgcc aaggttgctc gcggcggatc
     1381 gcgcagaacg cgagaaccaa ggggaccagc ccccgtacaa caagaacgcc ccctcgctgc
     1441 gagggggcgc cgacaatcac ttcgttgtgc gcttcttgcg tgcaggcttc accgtggggg
     1501 cctcgatcgt gcgggccacg tcggtgaagg gcttgaccgc ctcctggagc gcggccacgt
     1561 tggcttcgct gaggtcgatc tcgtaccact gcgtgtcacg gatctgacgg acaccgagct
     1621 tcgccgtgcc gtcttcgttg gtctctccgg tctcgtactc ctcctcgacc aaggccggca
     1681 cggcgagctt cacggtcgtg acgccgtccg tcgcctgggg gtcgaggtcg tcctggagga
     1741 tcgaccgcca gtacgtcgcc ttcttgatga cctcactgcc gaccggcacg ggagtgatga
     1801 cctcttgcac tcccggctcg ttcttctgag attcgtcgac ctggatggtt tccttcacgg
     1861 ccacaacggc gcccctttcg attggtgcag ccaaattcta aggtaaatgg gggagttgaa
     1921 gccaatcgag cccggcccgt gcgagaggcg cttgattgcg gatctccctg gtcaactctc
     1981 cgggtgatcc cggagtgccc acttcttttc gcgtgcggcg ccgttggtgg tcttcacaga
     2041 atggttgtga cgggcagagg tccagaaagc cttaaaggtc ctcggtgaag agcgagggtc
     2101 ctctttatcc cacgcgactt cgcgttcttc tcccgcggcc cggggaaccg cacgcacgat
     2161 gtgtgcaaag aggtcgggaa agcggcgaag cggacggccg ctcaggcggt gggggagatc
     2221 gcccagtagt gtgcgcgctc gtcggtgagg tagtggtggt cggccaggca ctcgatcacg
     2281 gtcagccgcc cctgcgccgc ccgggactcc aggaagccgt ggaaatccgt gacggagagg
     2341 tagttggcgc tcgtggtgag gagcaccagg ccgttcgggc gggtgaggcg caggagcgtg
     2401 tccaatgcgt tcggcgccac gtgcccggtg gtgaacacgc cgcagctgag ggtgaggtcg
     2461 taggtgtcgg gtgcgaattc gggcaggccc aggttgaggt cggtgccgct ggtgaggagg
     2521 cggtaggcgc ccgtccccgc ggcctgcgtg accatctcgt ggttgaggtc cagcccgtcg
     2581 aggctgccga tgccgagctt cttgaagacc gggccgacca gacccgttcc gcaccccgcg
     2641 tcgagcgcgg tggtctcctc gggggccggc agcatgcggg gcgcgagcga gaggtagaag
     2701 tcggccatgc gcagcggccc ctcgtaaccc atgttgctcg cgtcgctctc ataggtttcg
     2761 gcccagtcgc cgtagaaggc gctgagcagt tcggggcgcc cgtcgaggct gtgcgagcgg
     2821 tccacgatct cctggctgtc catgtgctgt ctcctccgca ccaaggcgcc tggccccata
     2881 ccgggccagg cgccttcgtg catcaggtgg gatgggtggt tcgtgcactg ggtcggctca
     2941 gacgagaccg atggtcacgg tgtcgcccgc ggccacgccg taccgttcca ggaagctgcc
     3001 ctggttgagg ccgagggcga ccttgccgcg gctgttgttg tagaccagcg gctcgccgat
     3061 cggcacctcg ccgaaggtgg cgtagtacgg gatctccacg gacagtccct cggccgtcac
     3121 ctggagggtc ttgccgtcca gtgccccgcc gccggacagc gcgtccaggg agatgttcgt
     3181 ccagacgttg ccgaacgcct tgtcgatccg gacgacttcg ccgcgcaccg aaccgtcgcc
     3241 gagctgcacg gccggggtcg tcggcagggt gaccagcttg gccacgtcga tcttgggtcc
     3301 gacggcggac ggggcgaccc cggcggccag gtgggcaccg caggccacca cgacgtcctt
     3361 gccgtaccag gtcggggtga cgggctggtt catcacgtcc ggcgaggtca cctcccacgc
     3421 ctcgacggcc gggacggcct tcagggccca ggtcagcagc ccgttgttgg gggcgacgag
     3481 cagctggccc ttctcgttgc ggaccacgac ggtgcgggtc gacgtgccgg tctcggggta
     3541 gacgtaggcg gcgatgacgg tgttggcggg gaacgacgcc ggtacgtcct gcaggtacag
     3601 gcccccctcc cgcacgtcga acggggtgac ctgatgggtg atgtcgacga tctctgcgcc
     3661 cggggcaatg gtcttgatga ggcccttgcc gagtgcgtgc gcctcgtcgt ggttaccgat
     3721 gtccgacagg taagcgatga tgggcttgtc caccgattcg accttccgtg gtccaggaat
     3781 gttccgattc cggccggaac ggatccggtg tgggtccggg attccggctg ggttttcatc
     3841 atcatgattc gtctcgccgg cccggcacca ttcggcgatg gtgacaaaat ccaatgccac
     3901 cttagggaca ttgccgtttg cgaccccggc cgtcgatgat ggggcgcgtg gcgggccgga
     3961 tcgctcacgg gcagggccgc gaaggcactg ttggcgtggg agagatgcgt cggtggcgga
     4021 atgtcggggc gccggcagcg gcgcgtgggc gccgccttgg gaacgtcgca tgacggctcc
     4081 gcacaccgaa gagagtcccg gagttcccgg ggggctcgcc gcgcactgtg ccgggagttc
     4141 tcggatgaat tccgccgtag agaggactgg attgcatgag tgtgcgtgaa accggacgag
     4201 tggccgtcgt aacgggtggt accaagggaa taggtctggc attcacccgg aagctcgtga
     4261 aactcggcta ccgggtcgtc gcgctgtacc ggcacgatcc cgaggcggcg cgggcggccg
     4321 ccgccgagct cggcgacgcc tgcacggtgc tccaggccga cgtctccgac agcgccgagg
     4381 tcaaccgact ggccgacggc gtgctggcgc accacggtgt gccgcacgtg ttggtcaaca
     4441 acgcaggccg gaacatcgac cggccgttcg ccgagatgtc ggacgacgcg tggcacacgg
     4501 tcctcaacac caacctgtcg ggccccttct acgtggcgcg ggcgttcgcc cccgcgatgc
     4561 gcgaggccgg cagcggcgtc atcgtcaaca tcgctgcgac gaccggcatc aggccccggt
     4621 tcaacgggat caactactgt gcgagcaagg ccgggctgct ccacctgacg aagtgcctgg
     4681 cgttggagct cgctccgcac gtccgcgtga acgcgctgat ccccggcatg atcgacaccg
     4741 aggacatgca gatgcgggtg cggaccgcgg cgcccgagca ccgcgcgagc atgctgacga
     4801 gcattccgca ggagcgcatc ggaacggtcg aggaaatggc ggcggcgctg gagttcctcg
     4861 tcggcgacgg ggccgagtac gtcacgggcc agaagctcat cgtcgacggc ggccagttca
     4921 tgtggtgacg gagtgtccgg aggacctgag ggggctgccg acggcggccc cctcacgccg
     4981 tcctggagcc gggaaccccc tcagcgtcgg gttatcacct gggtggtgaa ggtgacctgc
     5041 ccgttctggg tgccggtgat cgtggtgcgg gcgaggtccg gctgcggcca ctccaacggc
     5101 gtcgcctcga tccagcaggg cgcgtccaac tcggtgtagc gcgtgaaccg cgtgcggagc
     5161 gtcaccggtg tggtggggtc gggtccggcg aggcagtgcg cggcctggcc ggcggcctcc
     5221 agcaacacca tgccgggcag gtggtcgtgg gcgtggtcga agagcaccgg gtggtcggtg
     5281 tctacccgca gttgccagcg ccggggggcc gcagtcgggc agagcacgac gtccttggcg
     5341 cttgagcggc ccacatgcgc gggcggcacc ggagtgccgg ggagcggtgc gtgcgaggtc
     5401 gctgcccggg cgtcggcgta ctggccgcgc agacgctcgt agatggcggg tgagtggcag
     5461 gtgtagcgca ggtgcgccgc gcccaacagc tggccgtcgc ggaccgcggt gatctgtgcc
     5521 tccaggcgga cggaaccgcc cttgcggcgc gcggcgggca gggcgtgcgt gatggtcagt
     5581 tcgacctccg ccccgtgacc gtccggtggg gtgtccagca tggccggagt gagggtgcac
     5641 gagcagtagt cccagcccag ccggtactcc agcggcacct cgtaggccgc gttggagagc
     5701 agtgcgaggg cctggcgcac ggtctcgacc agtaacaggg agctgtagcg ccccgaacgc
     5761 ggcgtgtaga agctgtggtg ttgcggccag tgtgcggtga cgtggtgggt ggtgccggag
     5821 accgtgcgcc agtcagtgat cagtacttcg tcggagacgc gcttgcggac caggggaccc
     5881 aagtccgagg ccgcttcggg aagggaggcg acggggacgg cgaggggggc aggcatacga
     5941 cttctcctca agggtggtga agggtcgtgt gcagcaactg gtgtggaagg gcatggagtt
     6001 gaaccagccg tgtgccgtaa catagagaac gttatttatt ctgtcgagag cagaccggaa
     6061 agatgcatgt caagacagcg acaggaccgt gccctccaga cccgggccgc catagtgcgg
     6121 gcggcggccg aggtcttcgc ggagcagggc tacgcgggtg ccagcgtcac caagatcact
     6181 gaacgtgcgg gtctgacgat gggcgccatg tacttccact tcaagaacaa ggcggcgttg
     6241 gcgcgcgaga tagtcgtggg gcagccggat cgggtgctgc cgcggcacga gtccgtcggg
     6301 ctccagcgcg ccgtcgacat cactctgaca tgggcgcacc agatccgaga agaccccgtg
     6361 ctgctcgccg gtgcgcggct ggtgatggag caggaggagt tcatagggca ggagcagaac
     6421 tcccaccagc agtgggcgca gatattcgag caggagttgg ccgaagccca aaagcgcggt
     6481 gaactcacgg gaacgtgcga cgagtcggcg gtggcgcgcc tgatcgtcaa tgcgtgcacc
     6541 ggggcgcaga tgcatgccca catgttgact ggataccgcg atctgcccat ccgtgtggtg
     6601 gagatgtgga gctgcctgct cccgtcggtg gccacaccgg atgcggcggc ctcgatagac
     6661 ctgaacaccg agcgtgggag ggcgatatga cgaggcatca gtttcaatcg caatgagacg
     6721 ttgacccgag cgaggcgcgg cgagtgcact acggtggcga actgggattc gtataattcc
     6781 caactagtgg ctgtccacat acgggggcat gggatgacgc cgacgattcc agcacaggcg
     6841 acagggaccg caccgaggca gcagagcgcg gggcaggggc ggcgtgcgtc ccgtgggcgg
     6901 ccggcggtgt cgagacaccg cgaaggggat cgcggtcgtg gcacgctcaa tcgaaaaacg
     6961 atcatgataa ccggtgcgtc gagcgggatc ggggcggccg ccgcgcactg cttcgcggag
     7021 gaaggcgcat ccgtcgtcct gatggcccga cgcgaggacc ggctccgggc cctggccggg
     7081 gagatcgagg agaccggcgg ccaggtcgcg gtggtgaccg gcgacgtcac caaccaggct
     7141 gacgtcgacc gtgcggtcga cgtggcggtg gccgagttcg gcaccctcga cgccgccttc
     7201 aacaacgccg gacagggcac gctcggagct cccctgcacg agacggagca agcggtcttc
     7261 gaccggatca tggacgtcaa cgtccggggt gtctggaact gtctgcgtgc ccagatccgc
     7321 acgatgctca cccagggaac cggcggcgcg atcgtcaaca cctccagcac ggcgggcctg
     7381 ttcgcgaccg gggcggtcgc tccgtacgtg gccgccaagc acgccgtcct cgggctgacg
     7441 aagaccgcgg ccgccgagta cggcgaccac ggcatccgtg tcaacgccct cgtcgtgggg
     7501 gctacccgca ccgacatggt cacctccgtc ctgcaggagg tggacgggct ccgggaggcg
     7561 gacctcgtcg ccagggccat ccagcgccgg ctggccgacc ccgtcgaggt cgctcgcgcc
     7621 gctgcctggc tctgtagcga ccaggcgtcc ttcatcacgg gaggagtggt gcccgtggac
     7681 gggggctggt cggcggtctg agggcctcgg cccgtgggtg ggtcggcggt ggcggtcagt
     7741 agcggtagtg gtcgggcttg tacgggccct cgaccttgac gccgatgtag tcggcctgct
     7801 ccttgcgcag ggtggtgagc ttgacgccca gcgcgtccag gtggagccgg gcgaccttct
     7861 cgtccaggtg cttgggcagg acgtagacgt cggtcgggta ctgctcgggc ttggtgaaca
     7921 gctccagctg cgccagcgtc tggtccgcga acgagttcga catcacgaac gacgggtgac
     7981 cggtggcgtt gcccaggttc agcagacggc cctcggacag cacgatcaac accttgccgt
     8041 ccgggtgggt ccaggtgtgg acctgcggct tgacctcgtc cttgacgata cccggcagct
     8101 tcgccagacc cgccatgtcg atctcgttgt cgaagtgacc gatgttgccg acgatcgcct
     8161 ggtgcttcat ccgcgccatg tccgacgcaa ggatgatgtc cttgttaccg gtcgtggtca
     8221 cgaagatgtc agccgtctcc acgacctcgt ccagcgtcgc gacctggtaa ccgtccatcg
     8281 ccgcctgcag cgcacagatc gggtcgatct cggtgatgat cacgcgcgcg ccctggccgc
     8341 gcagcgactc cgcacaaccc ttgccgacgt caccgtaacc gcagaccacg gccgtcttgc
     8401 caccgatcag cacatcggtg gcgcggttga tgccgtcgat gagggagtgc cggcagccgt
     8461 acttgttgtc gaacttcgac ttggtcaccg cgtcattgac gttgatcgcc gggaagagca
     8521 gcacgccgtc acgctgcatc tcgtacagac ggtggacacc ggtggtggtc tcctccgtca
     8581 ccccacggat ctccgacgcc agccgggtcc acttctgcgg actgtcagcg agggtgcggt
     8641 tgaggagctc caggatcgcg tggtgctcct cgttctccgc ggtctccacc gccggagcct
     8701 tacccgcctt ctcgtactcg acgcccttgt gcaccagcag ggtggcgtca ccaccgtcgt
     8761 cgagaatcat gttcggacca ccggtggacg tgcccggcca ggtcagcgcc tgctccgtgc
     8821 accaccagta ctcctcaagg ctctcgccct tccaggcgaa caccggcaca ccctgcggcg
     8881 cctcgggcgt accgttcgga cccaccgcga tcgccgcggc cgcatggtcc tgggtcgaga
     8941 agatgttgca ggacgcccag cgcacctgcg cacccagggc gaccagcgtc tcgatcagca
     9001 cggcggtctg cacggtcatg tgcagcgaac cggtgatccg cgcgcccttc aggggctgct
     9061 gctcggcgta ctccttgcgg atcgccatca gaccgggcat ctcgtgctcg gcgagggtga
     9121 tctccttgcg gccgaaggcg gcgagggaaa ggtcagcgac cttgaaacgg gtggtgtcag
     9181 caggcatgga gactccttga acggcagaca tgaccatgga tgtcgagggc ggcgctcgac
     9241 cggttgaggg tgatgtagtg cagccccggt gcgccctcgt cgagcaaccg cagcgccatc
     9301 cgcgtggcat ggtccacgcc gatgcggtga ccgtccgcgg ggctgtcgcg tgcggcctcc
     9361 aggcggtgcg cgagttcctc ggggaaggcg gcgtggctga gttgggcgaa gcggcggatc
     9421 tgccgcacgt ccgtggccgg catgatctcg gggatgatcg gggtgtcgca gccggctgcc
     9481 gccacccggt cgcgcagccg caggtagtcc tcgacgtgga agaacatctg cgtgatggcg
     9541 tagtcggcac cggcccggca cttgccgatg aagtgccgga cgtcggtgtc cgggtccgac
     9601 gagcgcgggt ggccttcggg gaaggcggcg acgccgaccg agaacgagcc ggtgtccttc
     9661 acgagcgaga ccagttcgtg tgcgtgggtg agaccggcgg gatgcggcgt ccagggcccg
     9721 tcgggatccc ccggtggatc gccccgcagg gcgagcacgt cgcgcacccc cgcgtcggag
     9781 taccgcccga tgatgtggcg cagttcggcg acggagtggc cgacggccgt gaggtgcgcg
     9841 accgggcgga gggtggtgtc ggcggcgatc cgctcggtga gcgcgacggt gtggtctcgg
     9901 gaggaaccgc ccgcaccgta ggtgaccgac acgaacgtcg gtgccagcgc ctcgacgcgc
     9961 cggatcgtcc gccacagggt gcgcgtcccg gcctcggtcc tgggcgggaa gaactcgaag
    10021 gagaacgacg gtgccggacc ggtgacgtct gcacggagcg tgctcatgaa cggctccccg
    10081 cgttgaagta cgtggcttcc gggtggtgga tgacgatggc gtcggtggac tgctcggggt
    10141 ggagctggaa ctcctcggag agctggacgc cgatccgttc cggctggagg aggtcggcga
    10201 tcttggcgcg gtcctccagg tcggggcagg caccgtagcc gagggagaac cgggcgccgc
    10261 ggtacttcag cgcgaacatg tcctcgacgt cggccgggtc ctccccgccg aagccgagtt
    10321 cgccgcggac ccgggcgtgc cagtactcgg ccagcgcctc cgccaactgc acggacaggc
    10381 cgtgcagttc gaggtagtcg cggtaggagt tggcctcgaa cagctcggcg gtggccgcgc
    10441 cgatccgcga accgacggtg acgacctgga ggccgaccac gtcggtctcg ccggagtcct
    10501 cggggcggaa gaagtcggcc aggcacaggc gccgaccgcg ccgctgccgc gggaaggtga
    10561 accgcgtccg ctcggtgccg tcctcgccca gaatgatcaa gtcgtcgccc ttggaggtgc
    10621 aggggaagta gccgtggacg gcggcggctt cgagcaggtt cctggtgtgc aggaagccga
    10681 gccagccgcg cagtcgcggc cggccctcgg tctccaccaa ctcctcgtac gtgggaccgc
    10741 cgcccgtgcg cgctggacgc agcccccact ggcccttgaa cagggcgccc tcgtccagcc
    10801 aggaggcgta ctccttgagc tggatgccct tgatgacccg ggtgccccag aacggcgggg
    10861 tgggcaccgc attgtcgacg gccacgtcgg aacggatcgc gccctgctcc ggctccggct
    10921 ccgagacctg cacgtc
//
