LOCUS       NZ_CP104098            10885 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces noursei strain IFO14147 chromosome, complete genome.
ACCESSION   NZ_CP104098
VERSION     NZ_CP104098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Transcriptome and Metabolome Analysis Revealing the Improved
            epsilon-Poly-l-Lysine Production Induced by a Microbial Call from
            Botrytis cinerea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) College of life science, Huaibei Normal
            University, Dongshan road 100#, Huaibei, Anhui 235000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PGCGAP v. 1.0.34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025163575.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 12:09:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,195
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,883
            CDSs (with protein)               :: 7,883
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 93 of 218
            Pseudo Genes (incomplete)         :: 149 of 218
            Pseudo Genes (internal stop)      :: 11 of 218
            Pseudo Genes (multiple problems)  :: 33 of 218
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8048453
            Orig. end                         :: 8059338
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10885
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="27"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10885
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10885
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5885](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5885)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    150..165
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.53"
                     /tool="antismash"
     misc_feature    156..169
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.59"
                     /tool="antismash"
     misc_feature    complement(157..170)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.8"
                     /tool="antismash"
     gene            complement(243..1499)
                     /locus_tag="N1H47_RS35600"
                     /old_locus_tag="N1H47_35595"
     CDS             complement(243..1499)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576760.1"
                     /locus_tag="N1H47_RS35600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35595"
                     /product="cytochrome P450"
                     /protein_id="WP_016576760.1"
                     /sec_met_domain="p450 (E-value: 3.3e-18, bitscore: 64.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDPMESAEFSRDPYPLLAALRARGPVQRVRTGKGRTTWVVTGWA
                     EARAALADRRLSKDTARYFANRPSGRDLAPAVSQSMLATDPPDHGRLRKLAMAAFTPAA
                     VGRLEPRIREIAEGLAAELGRSAGAAGGPVDLVEGFAVPLPIAVICELLAVPEADRAAV
                     RRWSDDLFAAADPGTADRASHALAGYVAELIAARRAAPGDDVLSGLIAARDAGDRLSER
                     ELVSLAVLLVVAGHETTTHLIGNGTLALLRDDALHARLRDDPGLLPAAVEEFLRYEAPV
                     TLATFRYATEAFDLGGVRIGAGDVVLVSPGGANRDPARFDEPDAVRLARSGAGGHLSFG
                     HGPHHCLGAPLARAEARIAFEVLLARFPGLRLAAGGGQGAGGSDGPDGVTWRRTRLMRG
                     PAQLPVLLGPLREAPEGRG"
     gene            1670..1897
                     /locus_tag="N1H47_RS35605"
                     /old_locus_tag="N1H47_35600"
     CDS             1670..1897
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="N1H47_RS35605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35600"
                     /product="hypothetical protein"
                     /protein_id="WP_037635650.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     ADPAHAVSLDDPRLIEWRGGSLDDWPMPPA"
     gene            2021..3490
                     /locus_tag="N1H47_RS35610"
                     /old_locus_tag="N1H47_35605"
     CDS             2021..3490
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574648.1"
                     /locus_tag="N1H47_RS35610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35605"
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_016574648.1"
                     /transl_table=11
                     /translation="MSEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VTFFPTAAAAQGSGYRACRRCRPDAVPGSAEWNARADVVGRAVRLIGDGVVDREGVAGL
                     ASRLGYSARQLQRQLTAELGAGPTALARAQRAHTARVLLQTTGLPIAEIAFAAGFASVR
                     QFNDTIKEVYALTPSGLRAVRPGARSGPTPSSGTPGVLPLRLAFRGPYAARQVFDQLQA
                     QALGGIEEVTGVPGRRTYRRTLRLPNGTGIMEVDEVIGDGWLDCRLHLAELRDLTTATQ
                     RVRRLFDLDADPYAVGERLGADPVLAPLVARSPGLRSPGTVDPVELAVRAVLARRTGPA
                     AGRDLGERLVAGYGEPLPRPVGGLTHLFPRAVELARAPLAGLVPSPSRAAALRALAAAL
                     AARDVVLDAGTDRDRAERALRALPGIGPWTAGYVRMRALGDPDVLLGTPPPGTTPALWS
                     PWRSYATHYLHAADPAGTPARHSPQEERIDA"
     gene            3487..3996
                     /locus_tag="N1H47_RS35615"
                     /old_locus_tag="N1H47_35610"
     CDS             3487..3996
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574649.1"
                     /locus_tag="N1H47_RS35615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35610"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_016574649.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGERSATAPGGTALASLSVPDQKRGAVVQ
                     EGWTHDPAAFAEIARQLRAYFDGATAPFTVECVPRGTEFQQRVWTALEAVPRGTTTTYG
                     AIATAIGAPRGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLARKRLLLDLEG
                     AAADAA"
     gene            complement(4050..4814)
                     /locus_tag="N1H47_RS35620"
                     /old_locus_tag="N1H47_35615"
     CDS             complement(4050..4814)
                     /NRPS_PKS="Domain: PKS_KR (7-183). E-value: 2.7e-09. Score:
                     29.1. Matches aSDomain:
                     nrpspksdomains_N1H47_RS35620_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="N1H47_RS35620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35615"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_016574650.1"
                     /sec_met_domain="adh_short (E-value: 1.7e-29, bitscore:
                     102.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDLYGKTALVTGSSRGIGRGIAQRLAQDGALVAVHYGTNDIAAK
                     ETVESIQEAGGRAFSIAAELGVPGDAEALFAAFDAELSAAGAGPGLDVLVNNAAISLPG
                     HIDAVTPEEFDRTIAVNTKAPFFLIQHGLRRMRDGGRIVNVSSAVTSTAFPSTIAYGVS
                     KGAIDTLTRTLAKDVGDRGITVNTVAPGFIETDMNAAMRATPEAHAALAAISVFNRLGR
                     PTDIADVVAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        complement(4266..4793)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS35620_PKS_KR.1"
                     /evalue="2.70E-09"
                     /label="N1H47_RS35620_PKS_KR.1"
                     /locus_tag="N1H47_RS35620"
                     /protein_end="183"
                     /protein_start="7"
                     /score="29.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAQDGALVAVHYGTNDIAAKETVESIQ
                     EAGGRAFSIAAELGVPGDAEALFAAFDAELSAAGAGPGLDVLVNNAAISLPGHIDAVTP
                     EEFDRTIAVNTKAPFFLIQHGLRRMRDGGRIVNVSSAVTSTAFPSTIAYGVSKGAIDTL
                     TRTLAKDVGDRGI"
     CDS_motif       complement(4722..4787)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_N1H47_RS35620_0001"
                     /evalue="1.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="N1H47_RS35620"
                     /protein_end="31"
                     /protein_start="9"
                     /score="11.7"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAQDGA"
     gene            complement(5001..5885)
                     /locus_tag="N1H47_RS35625"
                     /old_locus_tag="N1H47_35620"
     CDS             complement(5001..5885)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574651.1"
                     /locus_tag="N1H47_RS35625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35620"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_016574651.1"
                     /sec_met_domain="AfsA (E-value: 7.2e-22, bitscore: 76.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGAFVHRPHAEDILIASWRRVDENHFTVDARWPHDHPYFVPSHG
                     RHHVMLAGETIRQAGLLLSHAEFGVPLGHHFILDDLVYTTDPQHHVLGDAPTRLTIDVL
                     CRKTRMRAGVLASSRFDMTLRAGGRIVATGHSSVNVTTPAVYRRLRGQRLAARRATGPA
                     GPSVAPELTGSVTEHDVLLSPTDRFGTWLLRIAPGHATVVNPENDHIPGLSLLDAAQQA
                     ARALTAPEQFVPYAFGTEFRRYAEHGIPCLIEARRTPCAIPGTTTVEVTGSQHGEPVFV
                     STLTALDMCRSPL"
     misc_feature    complement(6062..6075)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            6173..6874
                     /locus_tag="N1H47_RS35630"
                     /old_locus_tag="N1H47_35625"
     CDS             6173..6874
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947431.1"
                     /locus_tag="N1H47_RS35630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35625"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_037635647.1"
                     /transl_table=11
                     /translation="MARQDRAIRTRRVILEAAASVFDEFGFDRATIAEILTRAGVTKGA
                     LYFHFPSKEDLALAVLHEQVLDIAVRPQAIKLQEFVDAGQILAYRLRHDAIQRGAARLA
                     IEQSSSHLDRRQSMSAWSSFVEGLLKEAKARGEVMGSVNVRDAAELFVGAFAGLQMMSK
                     AFTDQNDLSQRLTVFFEHTLPTIAVPAILAKLRVDPERGEQLDAELQRHAVLEGEEHLG
                     EAGSNLLDVAP"
     misc_feature    7146..7160
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.9"
                     /tool="antismash"
     misc_feature    complement(7205..7220)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.69"
                     /tool="antismash"
     gene            7379..9805
                     /locus_tag="N1H47_RS35635"
                     /old_locus_tag="N1H47_35630"
     CDS             7379..9805
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="N1H47_RS35635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_35630"
                     /product="MFS transporter"
                     /protein_id="WP_016574653.1"
                     /transl_table=11
                     /translation="MTQTTTDQLPVRTADSAAGPPPAPLPASGHRRARAGLVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLGTASSNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LASLAVMVIGSLICGFTSQLLIMIVGRALQGFAMGAIPLGIGLMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPVAALIAQHADWHTLFFAAAGLGVLSMLLTLFAVPETSVRAPGRFD
                     IAGAVGLSAGLVALLLPITKGSDWGWASTRTLVLFGVAVVILVLWGVMELRIADPLVDL
                     RTSARREVLLTNLASITVGVAFYAISLVLPQLLQLPKSTGYGLGQSMVVAGLCVAPLGL
                     TMMVVAPVYARIAARRGPKVSLLLGMLIIGVGYGGGIGLMQAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSEPLGPVAVPTM
                     AGFRVSFLIATAAVALGVVVAAFLPSQRKTTGTRVAQSAEGAPAAGQESTAVRPTPPSP
                     GAVASEEPSAAGAPVAEQTEAAGEVGAGFRGRVVDSAGGPVAGATVTLIDRHGQQAGVT
                     TAAADGSYALAVPTAGTYVLTGAAPGHTPQAASATHYGEGTVAEVRLALVPTGSRLRGT
                     LRGGPDGAALTGAGVVVTDGRGDVVTCTSTDADGHWEVGQLPDGDYTLVLSAAGHQPEA
                     RAVQLTGGDGTDSPKRPGSQDVRLRPTATVRGTVRGPDGRPLADAAVTLLEDGTVTGHT
                     VTGADGAYAFADLKGRQYTLSAAGYPPHAEPISLTGGADERLDLDLAQPGAAGGR"
     misc_feature    complement(10344..10360)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.72"
                     /tool="antismash"
     misc_feature    10644..10661
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    10655..10672
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
ORIGIN
        1 acctccccgc accccgccac cgccgtgagc gcgacgacca gcgccagcgc gggcgccgga
       61 cgcatcggct tcggcttgcc cacgggcacc ctctcggtcg gacgggtcgg cggaccaggg
      121 gtaaccctgg aggtcgccag catcatacat tccgcttttt tcgaccaaat cgattcatag
      181 tgcacgagaa tgggcggggt gcgtacaagg ggcatccgcc ccgcccgcct ccgcgggtgc
      241 ggtcagcccc gaccctccgg cgcctcccgc aacggcccca ggagcaccgg cagttgggcc
      301 ggtccgcgca tcaaccgcgt gcgccgccac gtgaccccgt ccgggccgtc ggaacccccg
      361 gcaccctggc ccccacccgc tgccagccgc agtccgggga accgcgcgag caggacctcg
      421 aacgcgatcc gcgcctcggc gcgggccagc ggcgccccca ggcagtggtg cggaccgtgc
      481 ccgaacgaca ggtgcccgcc cgcgcccgac cgggccagcc gcacggcgtc cggctcgtcg
      541 aaccgcgccg gatcgcgatt ggcgccaccc ggggagacca gcaccacgtc ccccgctccg
      601 atgcggaccc cgccgaggtc gaacgcctcg gtggcgtagc ggaaggtcgc cagcgtcacc
      661 ggcgcctcgt accgcaggaa ctcctccacc gcggccggca gcagccccgg atcgtcccgc
      721 agacgggcgt gcagcgcgtc gtcacggagc agggccagcg tcccgttgcc gatcaggtgc
      781 gtcgtggtct cgtgcccggc gaccaccaac agcacggcca gcgagacgag ttcacgctcg
      841 gacaaccggt cgccggcgtc ccgggcggcg atcagcccac tgagcacatc gtccccgggc
      901 gccgcgcgcc gggccgcgat cagctccgcc acgtacccgg ccagggcgtg cgaggcccgg
      961 tcggcggtgc ccgggtccgc cgccgcgaac aggtcgtcgg accaccggcg caccgcggcc
     1021 cggtccgcct ccggcaccgc cagcagctcg cagatcaccg cgatcggcag cggcaccgcg
     1081 aacccctcga ccagatcgac cggaccgccg gccgcccccg ccgaccgccc cagctccgcg
     1141 gccagcccct ccgcgatctc ccggatccgc ggctccagcc ggcccaccgc cgccggggtg
     1201 aaggccgcca tggccagctt ccgcagccgg ccgtggtccg gcggatcggt ggccagcatg
     1261 ctctgactga ccgccggcgc cagatcgcgc ccggagggcc ggttggcgaa gtaacgggcg
     1321 gtgtccttcg acagccgtcg gtcggccagc gccgcgcgcg cctcggccca accggtcacc
     1381 acccacgtcg tgcggccctt cccggtccgc acccgctgca ctgggccgcg cgcccggagc
     1441 gcggcgagca gcggatacgg atcccgggag aactcggcgg attccatggg gtcgttcact
     1501 ggcgtcctcg gctctcgctc gcggttcggg tccgcgcccg ccgtcccggg ccggcccggg
     1561 accacgcccg caccggcgcc ggcggtgccg cccggccggg atcccaacca tccgggtgag
     1621 atcacgccgc cgacgtgcga gcgcgcggag cccggcgcac gctggaggca tgtccgagca
     1681 tccgcctgtc atcgtgcacc caccgacccc ctccggtgga cgccgggtga ccgtgcacgg
     1741 gcagatcgtc ggcctggccc acggccgcgg cgacgtcgcg gagttcctgc gccgcgcggg
     1801 cgtcgccgat ccggcgcacg ccgtctcgct ggacgacccc cgcctgatcg agtggcgtgg
     1861 cggctccctg gacgactggc cgatgccgcc ggcgtgacgg ccccgccccg gccggtggag
     1921 cgggcaccct ctgccgacca ccgaggcacc caccgcaccg gacgccatgt ccgatttccg
     1981 ccagagcgcg agcgcgggat ggcgcacagt ggggggtgtg atgagcgagg acagcaggta
     2041 cgaggcggtg cggagccggg acgccaggtt cgacggggcg ttcttcttcg cggtgtccac
     2101 caccgggatc tactgccggc cgagctgccc cgccaccacc ccgcggcggc gcaacgtcac
     2161 cttcttcccg accgccgcgg cggcccaggg ctccggctac cgggcctgcc ggcggtgccg
     2221 ccccgacgcg gtccccggct cggccgagtg gaacgcccgg gccgacgtcg tgggacgggc
     2281 ggtgcggctg atcggcgacg gtgtggtcga ccgggagggc gtcgccgggc tggcctcgcg
     2341 gctcggctac agcgcccggc aactccaacg gcaactgacc gccgaactcg gcgccgggcc
     2401 caccgcactg gcccgggccc agcgcgcgca caccgcacgg gtcctgttgc agaccaccgg
     2461 cctgccgatc gccgagatcg ccttcgccgc cggcttcgcc agcgtccggc agttcaacga
     2521 caccatcaag gaggtctacg ccctcacccc gagcgggctg cgggcggtcc gccccggcgc
     2581 ccgtagcggc ccgacaccct cgtccggcac gcccggcgta ctgccgctgc gactggcgtt
     2641 ccgcggcccg tacgcggccc ggcaggtctt cgaccagctg caagcgcagg cgctcggcgg
     2701 gatcgaggag gtcaccgggg tacccggccg ccgcacctac cggcggaccc tgcggctgcc
     2761 gaacggcacc ggcatcatgg aggtcgacga ggtcatcggc gacggctggc tggactgccg
     2821 cctccacctc gccgaactgc gcgacctcac caccgccacc cagcgcgtcc gccgcctctt
     2881 cgacctcgac gccgacccgt acgccgtcgg cgaacggctc ggtgcggatc ccgtgctggc
     2941 cccgctggta gcccgttccc ccggactgcg ctccccgggc accgtggacc cggtcgaact
     3001 ggcggtgcgg gccgtcctcg cccgccggac cggaccggcc gccggccggg acctgggcga
     3061 acgcctggtc gccggctacg gggagccgct gccgcgcccc gtcggcggcc tcacccacct
     3121 gttcccgcgg gccgtggaac tggcccgggc cccgctcgcc gggctcgtcc cgtccccgtc
     3181 gcgggccgcc gcactgcggg ccctcgccgc cgcgctcgcc gcccgggacg tcgtcctgga
     3241 cgccggcacc gaccgcgacc gggccgaacg cgccctgcgc gcactgccgg gcatcggccc
     3301 gtggaccgcc ggttacgtgc ggatgcgggc cctgggcgac cccgacgtcc tcctcggcac
     3361 accgcctccg ggcaccactc cggcactctg gagcccctgg cgctcctacg ccacccacta
     3421 cctccacgcg gccgaccccg ccggcacccc ggcccggcac tcgccccagg aagaacggat
     3481 cgacgcatga ccctgtacgc caccctcgac agccccctgg gcgaactcct gctggtgggc
     3541 gagcgatcgg ccaccgcgcc cggcggcacc gcactggcct cgctctcggt gccggaccag
     3601 aagcgcgggg cggtcgtcca ggagggctgg acccacgacc cggccgcgtt cgccgagatc
     3661 gcccgccagc tccgcgcgta cttcgacggc gcgacggcac cgttcaccgt cgagtgcgtc
     3721 ccccgcggca ccgagttcca acagcgcgtc tggacggcgc tggaggccgt cccgcgcggc
     3781 acgaccacca cctacggtgc gatcgccacc gccatcggcg ccccgcgcgg cgccgtccgc
     3841 gccatcggga cggccatcgg cgccaacccg ctcctggtgg tccgcccctg ccaccgcgtc
     3901 atcggcgccg acggctcact caccggatac gcgggcggcc tggcgcgcaa gcggctgctg
     3961 ttggacctgg aaggcgcggc agcggacgcc gcctgagagg gcgcggcccg ggcgctccgg
     4021 acgggggtcg gagcacccgg gcccgaccgt cacagcatcg acccgccggt cacgtcgatg
     4081 cgctggccgg tgatccaacg ggagtcgtcg gaggcgagga aggcgacgac gtcggcgatg
     4141 tcggtgggcc tgcccagccg gttgaacacc gagatggcgg caagtgccgc gtgcgcttcg
     4201 ggagttgccc gcatggcggc gttcatgtcg gtttctatga aaccgggtgc cacggtgttc
     4261 accgtgatgc cgcggtcacc gacgtccttg gcgagggtgc gggtgagggt gtcgatcgcg
     4321 cccttggaga ccccgtaggc gatcgtcgag gggaaggccg tgctcgtcac cgcggacgag
     4381 acgttcacga tccgcccgcc gtcccgcatg cgccgcagac cgtgctggat caggaagaac
     4441 ggggccttgg tattgaccgc gatggtccgg tcgaactcct ccggtgtcac ggcgtcgatg
     4501 tggccgggca ggctgatcgc ggcgttgttg acgaggacgt ccaggcccgg tcccgcgccg
     4561 gccgcggaca gttcggcgtc gaaggcggcg aacagcgcct cagcgtcgcc cgggacgccc
     4621 aattcggcgg cgatcgagaa cgcccgcccg ccggcctcct ggatgctctc gacggtctcc
     4681 ttggccgcga tgtcgttggt cccgtagtgg acggcgacca gcgctccgtc ctgggcgagg
     4741 cgctgcgcga tgccccggcc gatgccgcgg ctggatcccg ttaccagcgc ggtctttccg
     4801 tagaggtcac ccatggtggt tgtcggcctt ccgtagtgct gagcgcggtt cgttgtcacc
     4861 gtgatcggtg gatgcgcggg cacgtcgggc cgtccggcgg gtcaaccggc atggtgcggc
     4921 cgcccgggtc cggcccgacg ctcgcccgca cacggcacga gcccctgcgg ctcgtgaaat
     4981 cggatgtgag ccgtgcggac tcagaggggg gaccggcaca tgtccagtgc ggtcagggtc
     5041 gagacgaaga ccggttcgcc gtgttgggag ccggtgactt ccaccgtggt ggtgccggga
     5101 atcgcgcacg gggtccggcg cgcttcgatc aggcagggga tgccgtgctc cgcatagcga
     5161 cggaactccg tgccgaaggc atacgggacg aactgctcgg gcgcggtcag tgcgcgggcg
     5221 gcctgctgcg ccgcatcgag gagtgacagc ccggggatgt gatcgttctc ggggttgacg
     5281 accgtggcgt gtccgggcgc gatccgcagc agccacgtgc cgaaccggtc ggtgggggag
     5341 agcagcacgt cgtgctcggt gacgcttccg gtcaactccg gggccaccga agggccggcc
     5401 gggcccgtgg cgcggcgggc agcgaggcgt tggccccgga gtcggcggta gaccgcggga
     5461 gtggtgacgt tcacgctgga gtgaccggtg gcgacgatgc gccccccggc gcggagcgtc
     5521 atgtcgaatc ggctgctggc cagtacgcct gcccgcatgc gggtcttccg gcagaggacg
     5581 tcgatggtca gtcgggtcgg tgcgtcccca aggacatggt gttgcgggtc cgtcgtgtag
     5641 acgaggtcgt cgaggatgaa gtgatggccg agcggtacgc cgaactcggc gtgcgacagc
     5701 agcagacctg cctggcggat ggtttcgccg gcgagcatga cgtggtgccg gccgtggctc
     5761 gggacgaagt acgggtgatc atggggccac cgggcatcga cggtgaaatg gttctcgtcg
     5821 acccgccgcc acgacgcgat gaggatgtcc tcggcgtgcg ggcggtgcac gaacgcaccg
     5881 ggcacggcga gattcccgcc gcgctccgaa cagaccgcag cgggtcgact ccgcgcgggc
     5941 tgcgtgtgac gtcgggtcgt aggccgatct gtggcgctga cctgcaacgt gtcgctcatg
     6001 accgccccta ggcatcaata cagaggactt ccggacaacg gttaagatac gtaccatccg
     6061 gttttctttt caatgccgat gtcgaggtgc ggagcagctg tgaatctgga ggagacttag
     6121 ggcgtagcga attgctgaga ggctcaggcc tttgagggac gggaggcgtt ctgtggcacg
     6181 tcaggaccgc gcgattagga ctcggcgagt gatcttggaa gccgctgcgt cggtcttcga
     6241 cgagttcggc ttcgaccgcg cgaccatcgc ggagatcctc acgcgcgcgg gcgtgaccaa
     6301 gggtgccctg tatttccact tcccctccaa agaggatctt gccctcgcgg tgcttcacga
     6361 acaggtgctg gacatcgccg tacggccgca ggcgatcaaa ctgcaggagt tcgtggacgc
     6421 cgggcagatc ctggcctacc ggctccgcca cgacgcgatc cagcgtggtg ccgcccgtct
     6481 cgccatcgag cagagctcga gccaccttga ccgcaggcag tcgatgtcgg cgtggtcgtc
     6541 cttcgtcgaa gggctcctga aggaagccaa ggcgcggggc gaggtgatgg gcagcgtcaa
     6601 cgtacgggac gccgccgagt tgttcgtcgg cgccttcgcc gggcttcaga tgatgtcgaa
     6661 ggcgttcacc gaccagaacg acctgagtca acggctcacc gtcttcttcg agcacaccct
     6721 tcccaccatc gccgtgcccg ccatactcgc caagctccga gtcgatccgg agcgggggga
     6781 gcagctcgac gcggaactcc agcgccacgc cgttctggag ggcgaagagc accttggcga
     6841 ggcggggtcg aacctgttgg acgtggcgcc gtgacgcaag cggtggcccg tcgccccgca
     6901 gcagatccaa gggcggaagt tccgccgctg ggccggaaag tcacttctgt cccggcgtga
     6961 cgtcgagcgg aacgctccat ctttgacccg cttctgatcc ccccggccgg atgaatgtca
     7021 gtggcgtgcg cttcactgga tccagtgtca gtgacgaggc cgggggagag ccgatggctg
     7081 ggacggtgag cggaggcggg cgcggatcga ggggcgcgcg ggccggtacg gctctggagt
     7141 cgcagggggc gaccggccct gccgcgtacg gcagttggcg cagaccgggg cgatgcacga
     7201 ggggcgtgag ggagatcact gcccagggtg cgagacggcc cgctggcctg gaatagcccc
     7261 ttgcgttgaa acgttcgtca tgcacgtatc agggggtgta ttcccctgcc cgcgtagcgc
     7321 gcaccgcagc gcacggcgca ccctttcgct tgctcctgcc gccctggagg ccgcccgcat
     7381 gacccagacg acgaccgacc agcttcccgt gaggacggcg gactccgccg ccggaccgcc
     7441 gcccgccccg ctccccgcga gcgggcaccg cagagcgcgc gccggcctcg tccccgtcct
     7501 cgccttcgcc ggcatcgtcg tcgcggtgat gcagacgctg ctggtgccgg tcatcaagga
     7561 cctgccgacg ctgctcggca ccgcctccag caacgccacc tgggtcatga cggccaccct
     7621 cctcgccgga gccgtcgcca cccccatcat ggggcgcctc ggtgacctct acggcaagcg
     7681 ccggatgctg ctggcgagcc tggccgtcat ggtcatcggc tccctgatct gcggattcac
     7741 cagccagttg ctcatcatga tcgtcggccg ggcgctccag ggcttcgcga tgggcgctat
     7801 acccctcggc atcgggctga tgcgtgacga actgccccgg gagcggctcg gctcggccat
     7861 ggccctgatg agctcctcga tcggcgtcgg cggtggtctg gcgctgcccg tggcggcgtt
     7921 gatcgcccaa cacgccgact ggcacacgct gttcttcgcc gccgccgggc tcggcgtgct
     7981 gtcgatgctg ctcaccctct tcgccgttcc ggagacctcg gtgcgggcgc ccggacgctt
     8041 cgacatcgcc ggcgccgtcg gcctgtccgc cggactcgtc gccctcctgc tgccgatcac
     8101 caagggcagt gactggggtt gggcatcgac gcgcaccctc gtcctgttcg gggtcgccgt
     8161 ggtgatcctc gtcctgtggg gcgtgatgga gctgcggatc gccgatccgc tggtcgacct
     8221 gcgcaccagt gcccggcgcg aggtgctgct caccaacctc gcgtccatca ccgtcggagt
     8281 ggcgttctac gcgatctcgc tggtgctgcc gcagctgctc caactgccca agtcgaccgg
     8341 ttacggcctc ggtcagtcga tggtggtcgc cgggctgtgc gtggcaccgc tggggctgac
     8401 gatgatggtc gtggcgccgg tgtacgcccg gatcgcggcc cgccgcggcc ccaaggtctc
     8461 gctgttgctc ggcatgctga tcatcggcgt cggctacggc ggcgggatcg gcctgatgca
     8521 ggccccctgg cagaccgtga tcatcgcggt ggtggtcggc gcgggcatcg gcctggcgta
     8581 ctcctcgctg ccggcgctga tcatcggggc cgtcgacccg tccgagaccg gcgcggccaa
     8641 cggcctgaac accctgatgc gttcgatcgg cacctccgtg tccagcgccg tgatcggcat
     8701 ggtgctggcc cacatgtccg agccgctggg gccggtggcg gtgcccacga tggccgggtt
     8761 ccgggtctct ttcctgatcg ccacggccgc ggtcgcgctc ggcgtggtgg tggcggcctt
     8821 cctgccgtcc cagcggaaga cgaccggcac gcgggtggcg cagagcgcgg agggcgcgcc
     8881 ggccgccggc caggagtcga cggcggtccg gccgacgcct ccttccccgg gtgcggtcgc
     8941 gtccgaggag ccgagcgcgg ccggggcgcc ggtcgcggag cagaccgaag cggccgggga
     9001 ggtcggtgcg ggcttccgcg ggcgggtcgt cgattccgcg ggtgggccgg tcgcgggcgc
     9061 cacggtcacg ttgatcgacc ggcacgggca gcaggccggg gtcaccacgg ccgcggccga
     9121 cggcagctat gcgctggccg tcccgaccgc cggcacgtac gtcctcaccg gcgccgcccc
     9181 gggccacacc ccgcaggccg cctcggccac gcactacggc gagggcacgg tcgccgaggt
     9241 gcgactggcc ctggtgccca ccggcagccg gctccgcggc accctgcggg gcggtccgga
     9301 cggcgcggcg ctgaccggcg ccggcgtggt ggtcaccgac ggccggggcg acgtggtgac
     9361 ctgcacgtcg accgacgccg acgggcactg ggaggtgggc cagctgccgg acggtgacta
     9421 cacgctcgtc ctcagcgccg cggggcacca gccggaggcc cgtgcggtgc agctgaccgg
     9481 cggtgacggg acggactccc cgaagcggcc gggcagccag gacgtgcggt tgcggcctac
     9541 cgccacggtg cgcggtaccg tccgcggccc cgacggacgg ccgttggcgg atgccgccgt
     9601 gacgctgctg gaggacggca cggtcaccgg ccacaccgtc accggcgcgg acggcgccta
     9661 cgccttcgca gacctgaagg gccgtcagta cacgctgtcg gcggccgggt atccgccgca
     9721 cgcggagccc atatccctga ccggcggtgc agacgagcgg ctggacctcg acttggccca
     9781 accgggtgcc gccggcgggc gctgacgccc caccgcgacg acccccaccg cgacgaccct
     9841 ccccgtcggc cccgcccgcc gcggccgacg gggcgtcgtc ccggaccggc ggccggggga
     9901 gcctgcctgt catgggcggg gagtcgtcaa ctccgctttt ccgtaggcca gttgagaggt
     9961 ggaagaccca gccggggagg ggttgaggtc cggccctcga cggtggggct ccgcccggcg
    10021 ggcgtcgccc tgcggcaacc gtcggcccgc gcacgtaatc cccaggcggg actccgctcc
    10081 tacgcccgag cctctgcccc gccaccttcg cgctcgccgc ctccccgctg cctcctcccg
    10141 ggccccggca acggcccgcg ccactcccgt gcacccggcc gccatcgagc ggagcggagg
    10201 agtgccaccg aaggggtccg gtccggaaac gccgtcagtt gcggacggcg cggcgccaag
    10261 gtagcgcggc cgggtgaccg gtggcccgtg gccggaaagt gccgtcgccg tgcaaccccg
    10321 gcgtcaagga gagcgtctcg tccacaccct atggtgggat ttcggtcgag tgcggacccc
    10381 cggtccgccc cgggcgcgca gcgactccct cggcaccggc gctcggggat cggccctgcg
    10441 cacttaccag gggcatggga ccgcgaggtg gggcgcctgg gaattcatgg gttttctgag
    10501 gcccgtgagc aggaaggctg aaacggccga acctcgtagt gccgcgcccg caaccgcgcg
    10561 gatttctgcg agcctgggcg gcgcaccgga gttccgcctg gtcacaccgg gcgccggagc
    10621 agcgtcgagc cgggcgtgcg gcctcgttag cggggtgtta gtgcctcgtt agcggaccag
    10681 cagtgcccgg gagcacgctg gaagagcaac ggacggaccg tacgcaccca ctgatacgag
    10741 ggtacccacc aaccccagac gagggggact catgtcgctc accttcaaga ccctcggccg
    10801 cggccgccgt gtcgccgccg gatcgctgat cgccgtcacc gcgctgacgc tctccgcctg
    10861 ccagaacggc acggacggcg cgtcg
//
