LOCUS       NZ_CP104098            11321 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces noursei strain IFO14147 chromosome, complete genome.
ACCESSION   NZ_CP104098
VERSION     NZ_CP104098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Transcriptome and Metabolome Analysis Revealing the Improved
            epsilon-Poly-l-Lysine Production Induced by a Microbial Call from
            Botrytis cinerea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) College of life science, Huaibei Normal
            University, Dongshan road 100#, Huaibei, Anhui 235000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PGCGAP v. 1.0.34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025163575.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 12:09:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,195
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,883
            CDSs (with protein)               :: 7,883
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 93 of 218
            Pseudo Genes (incomplete)         :: 149 of 218
            Pseudo Genes (internal stop)      :: 11 of 218
            Pseudo Genes (multiple problems)  :: 33 of 218
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6648450
            Orig. end                         :: 6659771
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11321
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11321
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11321
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6321](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6321)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            348..1085
                     /gene="hemQ"
                     /locus_tag="N1H47_RS29100"
                     /old_locus_tag="N1H47_29100"
     CDS             348..1085
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536947.1"
                     /locus_tag="N1H47_RS29100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29100"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_016570336.1"
                     /transl_table=11
                     /translation="MDDMTDASTPDSAKTPNAGKKAKDLNEVIRYTLWSVFKLRDVLPE
                     DRSGYADEVEELFAQLAAKDVVVRGTYDLSGLRADADVMIWWHSENSDDLQEAYNRFRR
                     TRLGRALEPVWSNMALHRPAEFNKSHIPAFLADENARDYVSVYPFVRSYDWYLLPDEDR
                     RRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASEARRH
                     VREEIPFYTGRRKPVAELVAGLA"
     gene            complement(1169..2878)
                     /locus_tag="N1H47_RS29105"
                     /old_locus_tag="N1H47_29105"
     CDS             complement(1169..2878)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-295). E-value:
                     1.1e-17. Score: 56.5. Matches aSDomain:
                     nrpspksdomains_N1H47_RS29105_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536946.1"
                     /locus_tag="N1H47_RS29105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29105"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_051273444.1"
                     /sec_met_domain="PF00561 (E-value: 2.8e-23, bitscore: 81.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGALGSLVLTGLASPPAGGAVRDPAAPPAPVHPLAFGRC
                     AAVEHLPPTVECGTLTVPLDYARPNGKKIKLTVSRARATEPGARQGALVFNPGGPGASS
                     MQFPLYGALPHWQRTARAYDFVGYAPRGVGRSAPLSCQDPQEFAKAPTDSARYPSDAFK
                     RRKVAEARAYARGCAQRAGADLPFYNSINNARDLDMLRAALGEKKLTFMGASYGTYFGA
                     VYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLDQSLAFERRWHDWLRWVAKHHDTYHLG
                     THQEAVQHSYDTALARLRRTPLNGKIGPAQLQGAFLKTGYNDAFWSMRADALSKYLHGD
                     AKPLAAQAMPSGDGKDEENANAVYTAVECNDAAWPRDWRTWDRDNTALARTAPFETWDN
                     VWMNLPCAFWPDRRESAADGAEEALRTATDRAAKGGANGGELADAYRNAAATELDDALR
                     GQRGPLNIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERGAGTHGVAL
                     GDNACVNAHVEAYLLRGKVPGHRVYCAPRAEPVPGQPLRRARPSLIPTVKGR"
     aSDomain        complement(1994..2605)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS29105_Abhydrolase_1.1"
                     /evalue="1.10E-17"
                     /label="N1H47_RS29105_Abhydrolase_1.1"
                     /locus_tag="N1H47_RS29105"
                     /protein_end="295"
                     /protein_start="91"
                     /score="56.5"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGALPHWQRTARAYDFVGYAPRGVGRS
                     APLSCQDPQEFAKAPTDSARYPSDAFKRRKVAEARAYARGCAQRAGADLPFYNSINNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNL
                     DQSLAFERRWHDWLRWVAKHHDTYHLGTHQEAVQHSYDTAL"
     misc_feature    2832..2851
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            complement(3023..3865)
                     /locus_tag="N1H47_RS29110"
                     /old_locus_tag="N1H47_29110"
     CDS             complement(3023..3865)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577176.1"
                     /locus_tag="N1H47_RS29110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29110"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_016577176.1"
                     /transl_table=11
                     /translation="MWLLFLLLAWVVAVVSCVRVCRTALAAGLALHRGADATADADARA
                     DDTGADAGLTLYETAYLAGGPHRLADVVLVLMAQQRRLHLAHTGWATVVDPVGQDPVER
                     AALSAIGPEGQRRIPAVRRALTSAEAVRGLADRLVHAGLAAPAAVRTGIGAAVRHVQFA
                     ALLVLLAAGATYWAAPSGEVDRLVLAWFAFPLVLTLGTWAVARAEAHPYTAWATEAGSA
                     LLRHEHPGGPSRRPGPPPAEPAESTGPAEPTGLTGLALFGPTALADPGLRAALHSSGA"
     gene            complement(3993..4991)
                     /locus_tag="N1H47_RS29115"
                     /old_locus_tag="N1H47_29115"
     CDS             complement(3993..4991)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018554827.1"
                     /locus_tag="N1H47_RS29115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29115"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_020930145.1"
                     /transl_table=11
                     /translation="MSFTQGVYLGLGLSSAALVFGALLVRLRTPKARLVDSPTHDVREL
                     AFLAGGPARVAETVLAVMHDDGRLAVGGPGLVTVRRAAAHDPVEAAVLDAVAATPGGAL
                     TTVRERVMRSPAVQDVGDRLAARGLLRAPRATRGWRRWAGLQVKACGALFCVAMLLSFL
                     GVRDGGGPWAPPVVLRIVPAVAVGLVVGQVCRRSFGRRITRAGRSALAAARPRAKTPSG
                     FRDAEAAWQVAGLAVALGGAAVLTDEVLRDHFVQAKAAVAGSSGGASSAAGDSGGSGGA
                     PAWCGGSSDGGGGGCGASSCGGGSSCGGSSGCGGGSGCGGGGCGGGGCGGG"
     gene            complement(5001..6321)
                     /locus_tag="N1H47_RS29120"
                     /old_locus_tag="N1H47_29120"
                     /pseudo=""
     CDS             complement(5001..6321)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049032.1"
                     /locus_tag="N1H47_RS29120"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29120"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 1.1e-96, bitscore: 322.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGTGIGWRPEIAVEIAALPGVDWVEVVAENVCPGHLPDALGEL
                     RERGTTVVPHGVSLGLGGADRPDADRLAALAERATALDAPLVTEHLAFVRAGGPLTASP
                     VLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFAWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAAALDELPVEAIAYVHVAGGVERDGVWHDSHA
                     HPVTQPVLDVLAELCARIDPPGVLLERDDDFPEGGALAAELDAIRAVVEGARRVPAA"
     gene            complement(6528..7202)
                     /locus_tag="N1H47_RS29125"
                     /old_locus_tag="N1H47_29125"
     CDS             complement(6528..7202)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="N1H47_RS29125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29125"
                     /product="LysE family translocator"
                     /protein_id="WP_016578488.1"
                     /transl_table=11
                     /translation="MLTSLIGFTVVAALLTVSPGPDFAVVLRTALGSGRRAALCAAFGI
                     AAGCFVWGVAGAVGLTAMLAASKAAYDALRIAGALYLVWLGVQALRSARARRGTDGTAP
                     APDAAAADAPGDGGAPAVPGGSWRAFRGGLLTNVLNPKVGVVYLSLLPQFIPHGAPVVA
                     TTMLLVTVHAVLGVLWLGGVTLAVHRARAVLQRPRVRRRLDQATGGVLVALGAAVGFEV
                     AS"
     gene            complement(7270..8034)
                     /locus_tag="N1H47_RS29130"
                     /old_locus_tag="N1H47_29130"
     CDS             complement(7270..8034)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175670.1"
                     /locus_tag="N1H47_RS29130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29130"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_020930148.1"
                     /transl_table=11
                     /translation="MTTTETAGQAGAHHEPHQTDAPRPDRPEEGAPEYVLPLVVRMERD
                     APPGRTDALEAAARAVLVLLSDERALGTGEWAQVVHDWQDSRIRKVVRRARGAEWRRAA
                     ALPGITVSGREAEVRVFPPVPLDGWPKDLARLQVSGTELADPQAPGEPAAGAPVLWLNP
                     ALQMSAGKAMAQAGHGAQLAWWALSAADREAWRAAGFPLAVRTAAPAAWAGLTDSGLPV
                     VRDAGFTEIAPGSCTVVADHPALRGTALPAHR"
     gene            8181..9092
                     /locus_tag="N1H47_RS29135"
                     /old_locus_tag="N1H47_29135"
     CDS             8181..9092
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="N1H47_RS29135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29135"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_078487276.1"
                     /transl_table=11
                     /translation="MPWQTGQRMRLDGVGRRYGLRGPWVLREVQLDVPAGALLRVEGAN
                     GSGKSTLLRLLAGIDRPTVGRITGRPRTAYVPERFPAALPFTAAGYLTHLGRVHGLRTA
                     EATDLAAEWLARFGAAGHARTPMAELSKGTSQKVAVAQALLADPELLVLDEAWTGLDPA
                     ARDVLDRAVAERVAAGGTVVFVDHDPRRLAGAADGRFRVADGRLRSEAPVAAADAPREA
                     GPRTVIEAEGPPGAAPPTDLPGAPDLAPGPGGTLRLTVPAAHSDALLRTLLTARPPWHI
                     RAVTAAQEPGPHAAVPGGNPRP"
     gene            9089..9769
                     /locus_tag="N1H47_RS29140"
                     /old_locus_tag="N1H47_29140"
     CDS             9089..9769
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576543.1"
                     /locus_tag="N1H47_RS29140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29140"
                     /product="hypothetical protein"
                     /protein_id="WP_016576543.1"
                     /transl_table=11
                     /translation="MTAPLTALVRYQAALLARSYRWLPPLLVHAAFLAVGIQTGGPILD
                     AYGYAAGGLLPVTVWLTRICVTNEPPAARSCVAAATGPGRAHLAALLAAAGAALLLALV
                     GTAFVALLSDPHSTGHRVAVPVLPATVAGLCTALVCVVLGTAIGALCSRPVLLRPGWGV
                     PTGLIAALLVLVLGASPANAALTGMITGSERGTVTVPWLPLAAALLLASGAAAVASALA
                     GRRG"
     gene            9823..10020
                     /locus_tag="N1H47_RS29145"
                     /old_locus_tag="N1H47_29145"
     CDS             9823..10020
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972872.1"
                     /locus_tag="N1H47_RS29145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29145"
                     /product="hypothetical protein"
                     /protein_id="WP_016576542.1"
                     /transl_table=11
                     /translation="MPHPEPHPASEPSDQRPAAADPAAGSPPADCVECGKPTAYPATTP
                     GIVRCPVCEWQEAQRTACSG"
     gene            complement(10035..11120)
                     /gene="zapE"
                     /locus_tag="N1H47_RS29150"
                     /old_locus_tag="N1H47_29150"
     CDS             complement(10035..11120)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="zapE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088896.1"
                     /locus_tag="N1H47_RS29150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_29150"
                     /product="cell division protein ZapE"
                     /protein_id="WP_037634312.1"
                     /transl_table=11
                     /translation="MVASPASGDGTAGPTALASRAPHVPAERLVAEMVPPPRFQGARFD
                     TYLPDPGQPSQAAAVEVLRAFAAGTGGAERDAGGKRRWFSRAPKKPAAPKGPRGVYLDG
                     GYGVGKTHLLASLWHATPAAPELKAFGTFVELTNLVGALGFQQTVRTLSEHRLLCIDEF
                     ELDDPGDTVLVSSLLGKLVEAGVALAATSNTLPGKLGEGRFAAADFLREIQGLSAHFRA
                     LRIDGEDYRHRGLPEAPAPFDDETVRRAADRTPGASLDDFPALLDHLSKVHPSRYGAMC
                     DGITAVCLTDVQAVPDQSTALRLVVLADRLYDREVPVLASGKPFDALFSDEMLRGGYRK
                     KYFRAISRLTALARDAKTLVA"
ORIGIN
        1 ctgcgcacct ccctcggccg gcacggcgac gaggccgacc tgggacgcga cgacgacgaa
       61 ctggtcggcc tctccctggc ggacctcggc gaggcggtcg ggctggccgc ccggccggtg
      121 gcgtccacgg tcacccgctg ggacggcggg ctgccgcagt acgccgtcgg ccacgtggac
      181 cgcacggccc gcatccgggc cgcggtcgcc ggcctgccgg gcccgctgcg ggtctgcggc
      241 gcgctctacg acggcgtcgg catccccgcg tgcatcggct ccgccggccg ggccgccgac
      301 gagatcctcg ggaccctggc ggcgggctcc gccggcggcg agtgagaatg gacgacatga
      361 cagacgcttc cacccccgac tccgccaaga ctcccaacgc cggcaagaag gccaaggacc
      421 tcaacgaggt catccgctac accctctggt cggtcttcaa gctgcgcgac gtcctgcccg
      481 aggaccggtc cggctacgcc gacgaggtcg aggagctgtt cgcgcagctc gccgccaagg
      541 acgtggtggt ccgcggcacc tacgacctct ccggtctgcg cgccgacgcc gacgtgatga
      601 tctggtggca ctcggagaac tccgacgacc tccaggaggc gtacaaccgc ttccgccgca
      661 cccgcctggg ccgcgcgctg gagcccgtct ggtcgaacat ggcgctgcac cgccccgccg
      721 agttcaacaa gtcgcacatc ccggccttcc tggccgacga gaacgcccgc gactacgtga
      781 gcgtctaccc gttcgtgcgc tcctacgact ggtacctcct ccccgacgag gaccgccgcc
      841 ggatgctcgc cgaccacggc aagatggccc gcggcttccc cgacgtccgc gccaacaccg
      901 tcgcgtcgtt ctcgctcggc gactacgagt ggatcctcgc cttcgaggcc gacgagctgc
      961 accgcatcgt ggacctgatg cgccacctgc gcgcctccga ggcgcggcgc cacgtccgcg
     1021 aggagatccc cttctacacc ggccgccgca agccggtcgc cgagctggtc gccggcctgg
     1081 cctgaccggc cggcaccccg ggcacacgac ggacgggccg ccccgtgcag ggacggcccg
     1141 tccgtcgtgg acgccgggga cggggcggct accgcccctt caccgtcggg atcagcgacg
     1201 gccgggcccg ccgcagcggc tgccccggca ccggctcggc ccgcggggcg cagtacaccc
     1261 ggtgcccggg gaccttcccg cgcagcaggt acgcctcgac gtgggcgttc acgcaggcgt
     1321 tgtcgccgag cgcgacgccg tgggtgcccg cgccccgctc ggtgaccagc gacgagccgg
     1381 gcagccggcg ctggagttcc agcgcgcccg ggtacggcgt ggccgcgtcc cgttcggcgg
     1441 ccagcagcag caccggcggc agcgcgccgg gctcggtgcg gatgttcagc gggccgcgct
     1501 ggccgcgcag ggcgtcgtcc agctcggtgg cggcggcgtt ccggtaggcg tcggccagtt
     1561 cgccgccgtt cgccccgccc ttggccgccc ggtcggtggc cgtgcgcagc gcttcctcgg
     1621 cgccgtccgc cgccgactcc cggcggtccg gccagaaggc gcacggcagg ttcatccaga
     1681 cgttgtccca ggtctcgaac ggcgcggtgc gggcgagggc ggtgttgtcc cggtcccagg
     1741 tgcgccagtc gcgcggccag gccgcgtcgt tgcactcgac ggcggtgtag acggcgttgg
     1801 cgttctcctc gtccttgccg tcgccggagg gcatcgcctg cgcggccaac ggcttggcgt
     1861 cgccgtgcag gtacttcgac agggcgtccg cgcgcatcga ccagaacgcg tcgttgtagc
     1921 cggtcttgag gaacgccccc tgcaactgcg ccggcccgat cttcccgttc aacggggtgc
     1981 gccgcagccg tgccagggcg gtgtcgtagg agtgctgcac ggcctcctgg tgggtgccca
     2041 agtggtaggt gtcgtggtgc ttggcgaccc agcgcagcca gtcgtgccag cggcgttcga
     2101 aggcgaggct ctggtcgagg ttgttgcggt accagatctg ccggggctcc gggttgacca
     2161 cgctgtcgaa gatcatccga cgcacgtggc cggggaacag cgtcgcgtag accgccccga
     2221 agtacgtccc gtaggacgcg cccatgaacg tcagcttctt ctcgccgagc gccgcccgca
     2281 gcatgtccag gtcccgggcg ttgttgatcg agttgtagaa cggcaggtcg gcgccggccc
     2341 gttgggcgca gccgcgggcg tacgccctgg cctccgccac cttccgccgc ttgaaggcgt
     2401 cgctggggta ccgcgcgctg tccgtggggg ccttcgcgaa ctcctgcggg tcctggcacg
     2461 acagcggggc gctgcgcccc accccgcgcg gtgcgtagcc gacgaagtcg taggcgcggg
     2521 cggtgcgctg ccagtgcggc agcgcaccgt agagcgggaa ctgcatgctg gacgcgccgg
     2581 gtccgccggg gttgaagacc agggcgccct gtcgcgcgcc cggctcggtg gcccgggccc
     2641 ggctgaccgt cagcttgatc ttcttgccgt tggggcgcgc gtagtcgagg gggacggtga
     2701 gggtgccgca ttcgacggtg ggcggcaggt gctccacggc cgcgcaccgg ccgaaggcga
     2761 gggggtggac gggggcgggc ggtgcggccg ggtcccggac ggcgccgccg gccggtgggg
     2821 aggcgaggcc ggtcaggacc agcgacccga gggcgccgta caacgcaagt gctctcacga
     2881 gccgtccctt cgtatggtgc gcggtgcgcg gtgcggggct ccggagtggg gctcgcccgc
     2941 acggggccgc acggtgggca tacaaaaggg atagttgggc caattggtgg cgaagtaaag
     3001 caccctacgg cggtgtcgga cgctatgccc cggaagagtg gagcgccgcg cgcaaaccgg
     3061 gatcggcgag cgcggtgggc ccgaacaacg ccagtccggt cagtccggtc ggctctgccg
     3121 gcccggtcga ctctgccggc tctgccggcg gcgggcccgg acgcctcgac ggtccgccgg
     3181 ggtgctcgtg ccgcagcagc gccgaaccgg cctcggtggc ccacgcggtg tacgggtggg
     3241 cctcggcgcg ggcgacggcc caggtgccga gggtcagcac cagcgggaag gcgaaccagg
     3301 ccagcaccag ccggtcgacc tcgccggacg gcgccgccca gtacgtggcg ccggcggcca
     3361 gcagcaccag gagggcggcg aactgcacgt ggcggaccgc ggcgccgatc ccggtgcgga
     3421 ccgcggcggg ggccgccagc ccggcatgga ccagccggtc ggccagcccg cggaccgcct
     3481 cggcgctcgt cagcgcgcgc cgcacggccg gtatgcggcg ctgtccctcg gggcctatcg
     3541 cgctcagcgc ggcccgctcg acggggtcct ggccgaccgg gtccacgacg gtggcccagc
     3601 cggtgtgggc gaggtgcagc cgtcgctgtt gggccatcag gaccagtacc acgtcggcga
     3661 gtcggtgcgg gccgccggcg aggtaggccg tctcgtacag ggtcagaccg gcgtcggcgc
     3721 cggtgtcatc ggcccgcgcg tcggcgtccg cagtcgcgtc cgcgccgcgg tgcagggcca
     3781 gcccggcggc cagcgccgtg cggcagaccc ggacgcagga gacgaccgcg acgacccagg
     3841 cgagcaggag gaagaggagc cacatggcgg aggttctacg ggacggtggc ccctgccgcc
     3901 aaggagtttc tcgcagcgcg gacggatggc cgagatgtgt cggtgcaggc cgaaccgccc
     3961 gcaccggccg ggcacttcgg gccggcgccg cgtcatccgc cgccgcagcc gcccccgccg
     4021 caccctccgc cgccgcatcc ggagccgccc ccgcaacccg aactcccacc gcacgacgac
     4081 ccgccgccgc acgaggacgc accgcagccg cccccgccgc cgtccgagga cccaccgcac
     4141 cacgccgggg ccccgccgga accaccggag tcgccggccg cggacgacgc gccgcccgac
     4201 gaacccgcca ccgccgcctt cgcctgcacg aagtggtccc gcagcacctc gtcggtgagc
     4261 accgcggcac cgcccagcgc caccgccaac cccgcgacct gccaggccgc ctcggcgtcc
     4321 cggaacccgg agggcgtctt cgcccggggg cgggccgcgg cgagcgcgga cctgcccgcc
     4381 cgggtgatcc gccgaccgaa cgaccgccgg cacacctggc cgaccaccaa cccgacggcc
     4441 accgccggaa cgatccggag caccaccggc ggcgcccacg gacccccgcc gtcgcggacg
     4501 ccgaggaagg acagcagcat cgcgacgcag aacagcgcgc cgcacgcctt cacctggagg
     4561 cccgcccacc gccgccagcc ccgggtggcg cgcggggccc gcagcaagcc ccgggccgcc
     4621 agccggtcgc cgacgtcctg cacggccggg ctgcgcatca cccgctcccg gacggtggtc
     4681 agcgccccgc ccggggtggc ggcgaccgcg tccagcaccg ccgcctcgac cgggtcgtgc
     4741 gcggcggccc gccgcaccgt caccaggccc ggtccgccga ccgccagccg cccgtcgtcg
     4801 tgcatcaccg ccagcacggt ctcggccacc cgtgccggcc caccggccag gaacgcgagc
     4861 tcgcggacat cgtgggtcgg tgagtcgacc agccgggcct tcggcgtccg caaccggacc
     4921 agcagcgcgc cgaagaccag cgccgccgac gacaggccga gccccaggta caccccttgc
     4981 gtgaaggaca cgacgtcgcc tcacccgcgc cgcagggcgc gccgcagggt ccggacccgg
     5041 cgggcggcgg cccgcgcgag ccggggcacg gggtgcccgg acggcgggcg cggcccggcc
     5101 cggtcgcgcc accagtcggt cagccgccgc cgcgccaccg ggtcctcggg cgcgccctgg
     5161 gcgagcagga acgcggcgaa gtccagggcg tcctggcggt agccgccgtg catcgggcgg
     5221 tcctgggcgt agccgaggaa ggccgcccgg tagccgtcac cgaggaaccc cggcagctcg
     5281 ggcgcgacct tggcgaccac cgacgcccgc ttggcggcca gcgcccggcc ctgcacccgc
     5341 agccgcgccc ggtcgaaccc ctccggcgcc ggtgtcccgg ccaccagtgc cgacagcagc
     5401 gcggcctgcg ccagggccag ccgctcgcgg gccgcagagg tgtccgcggc cggacgttcg
     5461 gccgccgtcc ccgtcgtctc ctcaggccgc cgggacacga cgggccccct ccaccacggc
     5521 gcggatcgcg tcgagttcgg cggccagcgc cccgccctcg gggaagtcgt cgtcccgctc
     5581 cagcagcacg ccgggcggat cgatgcgcgc gcacagctcc gcgaggacgt ccaggaccgg
     5641 ctgggtcacc gggtgggcgt ggctgtcgtg ccacaccccg tcgcgctcga cgccgcccgc
     5701 tacgtggacg taggcgatcg cctcgaccgg cagttcgtcg agggccgcgg ccgggtcctc
     5761 gccgcggttg acgtggttgg tgtggaggtt ggcgacgtcg atgagcaggc gcactccggt
     5821 gcgctcgacc agctccgcca ggaattgccc ctcggtcagc tcctcgtcgg gccaggcgaa
     5881 cagcgcggcg atgttctcca gggccagcgg caccggcagc gcgtcctggg cgatccggac
     5941 gttctcgcac agcacgtcga gggcgtcccg ggtgcgcggc acgggcagca ggtgcccggc
     6001 ctccagcacc ggcgacgcgg tcagcgggcc gccggcccgg acgaacgcga ggtgctcggt
     6061 gaccaacggg gcgtccagcg cggtggcccg ctcggcgagg gcggccaacc gatcggcgtc
     6121 cggccggtcc gcgccgccga gcccgagcga caccccgtgc gggaccaccg tggtgccgcg
     6181 ttcgcgcaac tccccgaggg cgtcagggag atggccggga cagacgttct cggcgaccac
     6241 ctccacccag tccaccccgg gcagcgcggc gatctccacg gcgatctccg gccgccagcc
     6301 gattcccgtc cccaagcgca cggctccccc ttctcgtccc ctggtcgggg tattcctcac
     6361 cacgcgcggg ccggctaacc gcacggccga gcggttcaga ggttgatttg agcttggccc
     6421 agcacgtgcc gggacggcac ccgtcccggc cgtcccggcc cgttcgtcag ccggcaggcc
     6481 ggcaagtcag caagcaagtc agccagccgg tcccgggcgg gcgccgctca gctggcgacc
     6541 tcgaacccga ccgcagcgcc gagcgccacc aacaccccgc cggtggcctg gtccagccgc
     6601 cgtcgcaccc gtgggcgctg cagcaccgcc cgcgcccggt gcaccgccag ggtcaccccg
     6661 cccagccaca gcacgcccag caccgcgtgc acggtcacca gcagcatcgt ggtggcgacc
     6721 accggcgcgc cgtgcgggat gaactgcggc agcagggaca ggtagaccac gcccaccttg
     6781 gggttgagga cgttcgtcag caggccgccg cggaacgccc gccacgagcc gcccgggacc
     6841 gcgggcgccc cgccgtcccc cggtgcgtcc gccgctgccg cgtccggtgc cggcgcggtc
     6901 ccgtccgtgc cgcgccgggc ccgggcactg cgcaacgcct gcaccccgag ccacaccaga
     6961 tacagcgcac cggcgatccg cagcgcgtcg tacgccgcct tcgaggcggc gagcatcgcg
     7021 gtcagcccca ccgccccggc caccccccac acgaagcagc cggcggcgat gccgaacgcg
     7081 gcgcacagcg cggcccggcg acccgagccg agcgcggtcc gcaggaccac cgcgaagtcg
     7141 gggccggggg agacggtcag cagcgcggcg accacggtga agccgatgag cgaggtcagc
     7201 atgggcacac ggtatgtccc gaccggcacc cgaccgccgg cgggggagcg gcaccccgcc
     7261 ccgacccgct cagcggtgtg ccggcagcgc cgtaccgcgc agcgccgggt ggtcggcgac
     7321 caccgtgcac gagcccggcg cgatctccgt gaagccggcg tcccgcacca cgggcagccc
     7381 gctgtcggtg agccccgccc acgcggcggg tgccgcggtc cgcacggcca gcgggaaccc
     7441 cgccgcgcgc cacgcctccc ggtccgccgc ggacagcgcc caccacgcca gttgcgcacc
     7501 gtgcccggcc tgcgccatcg ccttgcccgc cgacatctga agcgccggat tgagccacag
     7561 caccggggcg ccggccgccg gctccccggg ggcctgcgga tcggccagct cggtcccgga
     7621 gacctggagc cgcgccaggt ccttcggcca gccgtccagc ggcaccggcg ggaagacccg
     7681 tacctcggcc tcccggccgc tgaccgtgat gccgggcagg gccgcggccc gccgccactc
     7741 cgcaccgcgc gcccgccgca ccaccttgcg tatccgggag tcctgccagt cgtgcaccac
     7801 ctgggcccac tcgcccgtcc cgagggcccg ctcgtccgag agcagcacca gcaccgcccg
     7861 cgcggcggcc tccagcgcgt ccgtccgccc gggcggggcg tcccgctcca tgcgcaccac
     7921 gagcggcagc acgtactccg gtgcgccctc ctcggggcgg tccggccgcg gggcgtcggt
     7981 ctggtggggt tcgtggtgcg cgccggcctg gccggcggtc tcggtcgtgg tcacgtgctt
     8041 cagtctgcca gccgccactg acagtccccg gcgaccgccc gcggtcgcgg aatccgctcc
     8101 cggcaccgcg cggcgccgcg tccccgacta ccgtcggcga gggccgggga accggcgggg
     8161 aagtggggga ggtgcagtcg atgccgtggc agacggggca gcggatgcgg ctggacgggg
     8221 tggggcgacg ctacgggctg cgcgggccct gggtgctgcg cgaggtgcag ctcgacgtgc
     8281 cggcgggcgc gctgctgcgc gtcgagggcg ccaacggcag cgggaagtca accctgttgc
     8341 ggctgctcgc gggcatcgac cggcccacgg tcggccggat caccgggcgc ccgcgcaccg
     8401 cctacgtccc cgagcggttc ccggcggccc tgccgttcac cgccgccggc tacctcaccc
     8461 acctcggccg ggtccacggg ctgcgcaccg cggaggcgac cgacctggcc gccgagtggc
     8521 tggcccgttt cggcgccgcc gggcacgccc ggaccccgat ggccgaactg tccaagggga
     8581 cgagccagaa ggtcgccgtc gcccaggccc tgctcgccga ccccgagctc ctcgtcctgg
     8641 acgaggcgtg gacgggcctg gacccggccg ctcgggacgt cctggaccgc gcggtcgccg
     8701 aacgggtcgc cgccggcggc accgtcgtct tcgtcgacca cgacccgcgc cggctggccg
     8761 gggccgcgga cggccgcttc cgcgtcgcgg acgggcggct gcggtccgag gcccccgttg
     8821 cggccgccga cgcgccccgg gaggcgggtc cgcggaccgt catcgaggcg gagggcccgc
     8881 cgggcgccgc gccgccgacg gacctccccg gcgccccgga cctggcgccc ggcccgggcg
     8941 gcaccctccg gctgaccgtc ccggccgccc actccgacgc cctgctgcgc accctgctga
     9001 ccgcgcgtcc gccctggcac atccgcgcgg tcaccgccgc ccaggagccg ggcccccacg
     9061 ccgccgtccc cggagggaac ccccgcccat gaccgccccg ttgaccgccc tggtccgcta
     9121 ccaggcggcg ctgctcgcca ggtcctaccg ctggttgccg ccgctcctgg tccacgcggc
     9181 gttcctggcc gtcggcatcc agaccggcgg cccgatcctg gacgcctacg gctacgccgc
     9241 cggcggactg ctgcccgtca ccgtctggct gacccggatc tgcgtcacca acgagccgcc
     9301 cgccgcccgc agttgcgtcg cggccgccac cggccccggc cgggcgcacc tggccgccct
     9361 gctggccgcg gccggcgccg cactgctcct cgcgctcgtg ggcaccgcct tcgtggcgct
     9421 gctctccgat ccgcactcca ccggtcaccg ggtcgccgtc ccggtgctgc ccgcgacggt
     9481 tgccgggttg tgcacggcgc tggtctgcgt ggtgctgggc accgcgatcg gtgcgctgtg
     9541 ttcccggccc gtgctgctgc gccccggttg gggcgtcccg accgggttga tcgcggcgct
     9601 gctcgtgctc gtcctgggcg cgtcgcccgc caacgccgca ctgaccggca tgatcaccgg
     9661 ctccgagcgc ggcaccgtca ccgtgccgtg gctgccgctc gccgccgcac tgctgctcgc
     9721 ctcgggtgcc gcggccgtgg cctcggccct ggccggacgc cgcggctgac cggtgcgctc
     9781 cggccggtcc ggggctcggg gccgcggcgt agggtcgggc gcatgccgca ccccgagccg
     9841 caccccgcgt ccgagccgtc cgaccaacgt ccggcggcgg ccgaccccgc cgcggggtcg
     9901 ccgcccgccg actgcgtcga gtgcggcaag ccgaccgcct acccggcgac cacccccggc
     9961 atcgtccgct gcccggtctg cgagtggcag gaggcccagc gcacggcctg ctcgggctga
    10021 ccgcccggcg gcgcctacgc gaccagcgtc ttggcgtccc gggccagggc ggtcaggcgg
    10081 gagatggcgc ggaagtactt cttgcggtag ccgccgcgca gcatctcgtc gctgaacagc
    10141 gcgtcgaagg gctttccgga ggccagcacc ggcacctcgc ggtcgtagag ccggtcggcg
    10201 agcaccacca ggcgcagcgc ggtcgactgg tccggcaccg cctggacgtc ggtgaggcag
    10261 accgccgtga tgccgtcgca catcgcgccg taccggctgg ggtgcacctt cgacaggtgg
    10321 tccagcaggg ccgggaagtc gtcgagcgag gcgccggggg tgcggtccgc cgcgcggcgg
    10381 accgtctcgt cgtcgaacgg ggcgggcgcc tcgggcaggc cgcggtggcg gtagtcctcg
    10441 ccgtcgatgc gcagggcgcg gaagtgggcg ctcaggccct ggatctcgcg caggaagtcg
    10501 gcggccgcga accggccctc gccgagcttg ccgggcagcg tgttggaggt ggcggccagc
    10561 gcgacaccgg cctccaccag cttgccgagc aggctggaga ccagcaccgt gtcgccgggg
    10621 tcgtccagct cgaactcgtc gatgcacagc aggcggtgtt cgctgagcgt gcgcacggtc
    10681 tgctggaagc cgagcgcgcc gaccaggttg gtcagctcga cgaaggtgcc gaacgccttg
    10741 agctcggggg ccgcgggggt cgcgtgccac agggaggcga gcaggtgggt cttgccgacg
    10801 ccgtagccgc cgtccaggta gaccccgcgc gggcccttgg gggcggcggg cttcttcggt
    10861 gcgcgggaga accagcgccg cttcccgccg gcgtccctct ccgccccgcc ggtgcccgcg
    10921 gcgaaggccc gcaggacctc cacggcggcg gcctggctgg gctgtcccgg gtccgggagg
    10981 taggtgtcga agcgggcgcc ctggaagcgc ggcggcggca ccatctcggc gaccaggcgc
    11041 tcggccggga cgtgcggcgc gcgggaggcg agcgcggtgg ggcccgcggt gccgtccccc
    11101 gaggccgggg acgcgactat cggggaggga gcggaggagg actgggaagg tgacacagcc
    11161 gtccagccta gccgctgtga cgcaccaccc gacccgctga gcgaggcatg tcacactgcg
    11221 cccatgcgac gcctgttccc cctccccgcc ccgcccgcac cggccccgga cgccctcgtg
    11281 gacgtcccgg agggcgccga ccggaccgtc ggccccgacg g
//
