LOCUS       NZ_CP104098            10411 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces noursei strain IFO14147 chromosome, complete genome.
ACCESSION   NZ_CP104098
VERSION     NZ_CP104098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Transcriptome and Metabolome Analysis Revealing the Improved
            epsilon-Poly-l-Lysine Production Induced by a Microbial Call from
            Botrytis cinerea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) College of life science, Huaibei Normal
            University, Dongshan road 100#, Huaibei, Anhui 235000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PGCGAP v. 1.0.34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025163575.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 12:09:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,195
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,883
            CDSs (with protein)               :: 7,883
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 93 of 218
            Pseudo Genes (incomplete)         :: 149 of 218
            Pseudo Genes (internal stop)      :: 11 of 218
            Pseudo Genes (multiple problems)  :: 33 of 218
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2410752
            Orig. end                         :: 2421163
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10411
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="14"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10411
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10411
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5411](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5411
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(102..117)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.39"
                     /tool="antismash"
     misc_feature    complement(311..326)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            complement(423..836)
                     /locus_tag="N1H47_RS10690"
                     /old_locus_tag="N1H47_10690"
     CDS             complement(423..836)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536880.1"
                     /locus_tag="N1H47_RS10690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10690"
                     /product="hypothetical protein"
                     /protein_id="WP_020929958.1"
                     /transl_table=11
                     /translation="MTTADPAPTSAPAAAPTGDPSSAGPLRTLFCIGVTQDFFAADDDR
                     RAAVAAAIPPAFDRLGARFGVTVLGTLDDDQLMVGATTAWPWTSYVLADVPDLDTVAAV
                     CGIVRDTPVGDGRLWRYLRIEARVGRPLFFATA"
     misc_feature    complement(911..924)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.04"
                     /tool="antismash"
     gene            984..1364
                     /locus_tag="N1H47_RS10695"
                     /old_locus_tag="N1H47_10695"
     CDS             984..1364
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576770.1"
                     /locus_tag="N1H47_RS10695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10695"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_016576770.1"
                     /transl_table=11
                     /translation="MPRTDPGGAPGTDPSATSAPADPDQPETARIQLGDLLQAFSDPLR
                     RRIVRRLATQGEAGCGDIELPVTRATGSYHFRILRTAGWTHTRRAGRERYISLRRTDLE
                     TRFPGLVDALLRADAAERDTPA"
     gene            complement(1411..2502)
                     /locus_tag="N1H47_RS10700"
                     /old_locus_tag="N1H47_10700"
     CDS             complement(1411..2502)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576771.1"
                     /locus_tag="N1H47_RS10700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10700"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_016576771.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1e-17, bitscore:
                     63.2, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAALHLKGRVLVGPDEVRDELWVIDGRVTFERPSVARDVTTV
                     SGWVLPGLVDAHCHVGLDAHGPVDAATSEKQALTDRDAGTLLIRDAGSPSDTRWIDDRP
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAQEARRGDGWVKLVGDWIDRSVGD
                     LAACWPPGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEDTIPLF
                     AERQVAIVPTLVNIATFPQLADGGEAKFPRWSAHMRRLHERRYDTVRAAYDAGVPIFAG
                     TDAGGSLPHGLVADEVAELMKAGLPVEAALSATTWGARTWLGRPGLTEGAPADLVVFET
                     DPRADVRVLAAPRLVVLRGRIVG"
     misc_feature    2840..2854
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.24"
                     /tool="antismash"
     misc_feature    complement(2864..2879)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(2874..2889)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    2922..2937
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.88"
                     /tool="antismash"
     gene            3044..3610
                     /gene="ectA"
                     /locus_tag="N1H47_RS10705"
                     /old_locus_tag="N1H47_10705"
     CDS             3044..3610
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="N1H47_RS10705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10705"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_016577188.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIKEIPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSAVARDATGEPAAFVTGYLRPDRPDTLVVWQIAVDAAHRGR
                     GLAAALLDGLTARAIDELGVRSVETTITPDNDASNRLFASFAARHSVPVKREVLFDAAL
                     FPEQGHEPEVLHLIGPFETPPGSNR"
     gene            3679..4947
                     /gene="ectB"
                     /locus_tag="N1H47_RS10710"
                     /old_locus_tag="N1H47_10710"
     CDS             3679..4947
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 8.4e-74.
                     Score: 240.5. Matches aSDomain:
                     nrpspksdomains_N1H47_RS10710_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="N1H47_RS10710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10710"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_016577187.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-76, bitscore:
                     257.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRLTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGQTPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     DWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLGRGEIVEGHLK
                     AIVAEHPGAFAEYRGRGLVWGLECTDKELANKIAKRAFELGLLIETSGPQSEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCA"
     aSDomain        3763..4773
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS10710_Aminotran_3.1"
                     /evalue="8.40E-74"
                     /label="N1H47_RS10710_Aminotran_3.1"
                     /locus_tag="N1H47_RS10710"
                     /protein_end="365"
                     /protein_start="28"
                     /score="240.5"
                     /tool="antismash"
                     /translation="FDRAQGSRLTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGQTPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARADWLRALAALCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLGRGEIVEGHLKAIVAEHPGAFAEYRGRGLVWGLECT"
     gene            5001..5411
                     /locus_tag="N1H47_RS10715"
                     /old_locus_tag="N1H47_10715"
     CDS             5001..5411
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577186.1"
                     /locus_tag="N1H47_RS10715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10715"
                     /product="ectoine synthase"
                     /protein_id="WP_016577186.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.7e-51, bitscore:
                     171.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCTEGVAELTNDETGEKHLITPGVMYLLNGHEKHTMRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPEPEAS"
     gene            5492..6394
                     /gene="thpD"
                     /locus_tag="N1H47_RS10720"
                     /old_locus_tag="N1H47_10720"
     CDS             5492..6394
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="N1H47_RS10720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10720"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_016577185.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-35, bitscore: 122.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTSEVITPRQDPVVWSQPGAEGPFAASELS
                     DFDRDGFFAMPELLTAGEVAVYRAELDRLVNDPATRADPRSIVEPQSQDVRSVFEVHRI
                     SDVFARLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHRHFVGCEGATPKDNYKKSLQMQDAGTPSDGT
                     LTTLADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSMENTAVEPFA
                     APVRRPEYIGARAPFAPVK"
     gene            complement(6524..7594)
                     /locus_tag="N1H47_RS10725"
                     /old_locus_tag="N1H47_10725"
     CDS             complement(6524..7594)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574047.1"
                     /locus_tag="N1H47_RS10725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10725"
                     /product="alkene reductase"
                     /protein_id="WP_016574047.1"
                     /transl_table=11
                     /translation="MTTAFDPIELAGIPLANRIVMAPMTRNRATEAGVPTPSMVTYYTQ
                     RASAGLIVTEATQTEVVGRGYPLTPGLHTAEQVAGWRRVTDSVHAAGGRIFAQLWHGGR
                     IGHPVLLPDGLEPVGPSAVRAAGQGFTQNGPTDFVTPRELTDEEIRATVDGFAAAARNA
                     IEAGFDGVEIHGANGYLVHQFLAPNANLRTDAWGGSDAARARFAIEVVKAVAEAVGAHR
                     TGLRLSPGNPFNDIAEPEPEATYTALLDAVSPLGLAYLHTCDVHDLQLLARLRKRFDGP
                     FLLNPATVGRPTGPEELSLVEQGDADLLAYGALFIANPDLPRRLAAGGPFNVPERASFY
                     GGDDHGYLDYPSLDDN"
     gene            complement(7737..8810)
                     /locus_tag="N1H47_RS10730"
                     /old_locus_tag="N1H47_10730"
     CDS             complement(7737..8810)
                     /NRPS_PKS="Domain: Aminotran_5 (16-350). E-value: 3.7e-20.
                     Score: 64.1. Matches aSDomain:
                     nrpspksdomains_N1H47_RS10730_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574048.1"
                     /locus_tag="N1H47_RS10730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10730"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_016574048.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.5e-12, bitscore:
                     44.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHTALGGDEFAPETVYLNSASSGLLPARTAAAVRDALAESASH
                     GTMGRDYFGTADRARGVFARLMGVPAERVAVGSSVAVRSAFVAASLPAGSEVLVAEGDF
                     SSLVNPLMAHGGCTVRAVPREALAEAVRPRTALVALSAVHPQDGRIADLAAVRDAARAH
                     GALTYVDVTQAAGWLPVPLADFDYAVCGAYKWLLCPKGTTFTVFGGERGELGGPGWPVP
                     THAGWVAGEDPGESNYGPVDRPAPTARRYDEPHANYAYVGAEHSLGLLAEIGLATIHAH
                     DVALAEQYRAGLVAAGFTPRAAPGSAIVSTPGLADAEPRLAAAGVRVAVRGGLLRAAFH
                     LYNSRADVDRALELLVP"
     aSDomain        complement(7761..8762)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS10730_Aminotran_5.1"
                     /evalue="3.70E-20"
                     /label="N1H47_RS10730_Aminotran_5.1"
                     /locus_tag="N1H47_RS10730"
                     /protein_end="350"
                     /protein_start="16"
                     /score="64.1"
                     /tool="antismash"
                     /translation="VYLNSASSGLLPARTAAAVRDALAESASHGTMGRDYFGTADRARG
                     VFARLMGVPAERVAVGSSVAVRSAFVAASLPAGSEVLVAEGDFSSLVNPLMAHGGCTVR
                     AVPREALAEAVRPRTALVALSAVHPQDGRIADLAAVRDAARAHGALTYVDVTQAAGWLP
                     VPLADFDYAVCGAYKWLLCPKGTTFTVFGGERGELGGPGWPVPTHAGWVAGEDPGESNY
                     GPVDRPAPTARRYDEPHANYAYVGAEHSLGLLAEIGLATIHAHDVALAEQYRAGLVAAG
                     FTPRAAPGSAIVSTPGLADAEPRLAAAGVRVAVRGGLLRAAFHLYNSRADVDR"
     gene            complement(8884..9588)
                     /locus_tag="N1H47_RS10735"
                     /old_locus_tag="N1H47_10735"
     CDS             complement(8884..9588)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579040.1"
                     /locus_tag="N1H47_RS10735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10735"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_016579040.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHRADVEVVHRSFELDR
                     DAPPATDVPILDMLASKYGVSRAQAEEMESRVAEAAAGEGLGYRSDRIHGNTFDLHRLL
                     HLAKAHGVQDALLTALYHANFAEARQLGDPAVLTGIAVEAGLPADEAARVLADPDAYAA
                     EVRTDERTAAELGATGVPFFVIDRRYGISGAQPVDVFHQALQRAHADSAVQVLAAGDAD
                     VCGDGGCAVPGA"
     gene            9726..10409
                     /locus_tag="N1H47_RS10740"
                     /old_locus_tag="N1H47_10740"
     CDS             9726..10409
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536870.1"
                     /locus_tag="N1H47_RS10740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_10740"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_020929960.1"
                     /transl_table=11
                     /translation="MGTPAPHGTDGFWETTGAAKTFTHPIDPALLAEFVPRTARVLDYG
                     CGYGRLTAELVGLGYGAVRGVDRSAALIARGRQAHPGLALLRWSGFPLPFAAGSFDAAL
                     LFAVLTCVPDDADQRAIVGELARLVRPGGVLYVSDVPLQDDPFNRARYERFAARFGTYG
                     VFQTPDGGVFRHHPPPWLRGLLRAAGFAVLRECRTVVPTLDGRTAERLQLMTRREPAVA
                     GPAPG"
ORIGIN
        1 ccgcccgaag ccgcgctcgc gcatgtcggc accgaccgcg cgggtcagca ggaagaccgc
       61 gcggaggttg accgcgaaga cctcgtccca ctcctcgggg gtgatctccc acagcgtccg
      121 ggcgagcggc cgggcggcgt tgttgatcag cacgtcgacc gggcgccagg cccggacgtc
      181 gtcgtaggcg gcggcgaggg cgtccacgtc gcgcaggtcg gcgtggaccg gccggatccg
      241 ttccccggcg gggtcgagcc gctcggcgag ggcacggttg gcctcctcgt cgacgtccag
      301 cgcggccacg tgggcgccct cggcgtggca ggcgcggacc agggcggcgc cgagcccctg
      361 ggcgctgccg gtgatcagga cggtccggcc gtcgagtccg gtgtcgagca cggtgatccc
      421 cctcaggccg tggcgaagaa gagcgggcgg ccgacgcggg cctcgatgcg caggtagcgc
      481 cagagccgcc cgtcgccgac cggggtgtcg cggacgatgc cgcacaccgc ggcgacggtg
      541 tccaggtcgg ggacgtcggc gaggacgtag gaggtccacg gccaggcggt ggtggcgccg
      601 accatcagct ggtcgtcgtc cagggtgccg agcacggtga cgccgaagcg cgcgccgagc
      661 cggtcgaagg ccggcgggat cgcggcggcc acggcggcgc gccggtcgtc gtcggcggcg
      721 aagaagtcct gggtcacgcc gatgcagaac agcgtgcgca gcgggcccgc ggaggacggg
      781 tccccggtgg gggcggctgc gggggcggag gtgggtgccg ggtcggcggt ggtcatcgcg
      841 gagcctcgcg ggagcgttcg gtgtcgacgg gcgggcccgg ctgtgggtgg tgtcgggcgc
      901 cgtcgccgtc ggtgaataat tagaggatcg tcaacgcatc gacgatcacc gaccgtatca
      961 ctcgtttaga gagttctcga acaatgcctc gtacggaccc gggcggcgcc cccggcacgg
     1021 accccagcgc cacctccgcg ccggccgacc ccgaccagcc cgagaccgcg cgcatccagc
     1081 tcggggacct gctccaggcg ttcagcgacc cgctgcggcg acggatcgtg cgccggctcg
     1141 ccacccaggg cgaggcgggc tgcggggaca tcgaactgcc ggtgacccgg gccaccggca
     1201 gctaccactt ccggatcctg cggacggcgg gctggacgca cacccgccgc gccggccgcg
     1261 agcggtacat ctcgttgcgc cgcaccgacc tcgaaacccg cttccccggc ctggtggacg
     1321 cgctgctgcg ggccgacgcg gcggagcggg acaccccggc gtaacccgcg gcgcccggcg
     1381 cgtgagccgc ccggcgccgc ccgcggcggc tcagccgacg atccggccgc gcagcaccac
     1441 caggcggggc gccgccagca cccggacgtc ggcccgcgga tcggtctcga agacgaccag
     1501 gtcggccggg gcgccttcgg tcaggccggg gcggcccagc caggtgcggg cgccccaggt
     1561 cgtcgccgag agggcggcct cgacgggcag cccggccttc atcagctcgg ccacctcgtc
     1621 ggcgaccagg ccgtgcggca gcgagccgcc ggcgtcggtg ccggcgaaga tcggcacccc
     1681 ggcgtcgtag gcggcccgca ccgtgtcgta gcgccgctcg tgcagccggc gcatgtgcgc
     1741 ggaccagcgc gggaacttcg cctcgccacc gtcggcgagc tgcgggaagg tcgcgatgtt
     1801 gaccagggtg gggacgatgg cgacctgccg ctcggcgaag agcgggatgg tgtcctcggt
     1861 caggccggtg gcgtgctcga tgcagtcgat cccggcctcg accagcgggg cgagggactc
     1921 ctcggcgaag cagtgcgcag tgacccgggc gccgagccgg tgcgcctcgg cgatggccgc
     1981 ggccacctcg ccgggcggcc agcaggcggc cagatcgccc accgaacggt cgatccagtc
     2041 gccgacgagc ttgacccagc cgtcgccgcg ccgggcctcc tgggccacgt aggcgaccag
     2101 gtccgccggc tcgatctcgt gcgcgtagtt gcggatgtag cgccgggtgc gggcgatgtg
     2161 ccggccggcg cggatgatcc gcggcaggtc ggggcggtcg tcgatccagc gggtgtccga
     2221 gggcgagccg gcgtcccgga tcaggagggt gccggcgtca cggtcggtga gcgcctgctt
     2281 ctcgctggtg gccgcgtcca ccggtccgtg cgcgtcgagc ccgacgtggc agtgggcgtc
     2341 gaccagcccg gggagcaccc accccgacac cgtcgtgaca tcccgtgcca ccgaaggtcg
     2401 ctcgaacgtc acccggccgt cgatgaccca cagctcgtcg cggacctcgt ccggcccgac
     2461 gagcaccctc cccttgaggt gcagcgccgc accttcactc atggacgtca tgcaggcact
     2521 gtatgcgggg gccgcggcgg gcgaccacgg tgcgccgggc gcccgccgcg gcccgcccgg
     2581 cgggcgattt cgcaacaatc acgccccggt atccgcggta tgcgcgcccc gccgcttcgc
     2641 gtaacacgcc gcaacacttc ccgcatctct tcggggtctc ttcggggtgg aactcaggtg
     2701 acttctcgtg tccgttttgg tgaatcgtcc gcgatacctt ccggcgacga attcccaatt
     2761 ccccagaagc cagcttcccg tattcgtatg cccgctttcc cggacctcaa acgccaagat
     2821 ttcgctagcg tcgagtctca ttattcaggc acttcacggg agcgttactg agctgtgacc
     2881 gactacacaa cgtgtccgtt tcgtcccgaa cttccgtgac gaatcgtgac aattttccac
     2941 cggatcgcgc gctcgcgcac ccccgcgtga taacacacag acgcctccgg cgtaacccag
     3001 tcccgcgatg caatttggtt ttcggctggg taaattcaat tgcatgaccg ccgcacaagc
     3061 agaccatgcc ggtgcccgaa gcgatatcaa ggaaattccg gagggcttca agctcgacac
     3121 cccccgggtg gaggacgggg cagcgatctg gcgcatcgcc cgcgactcga aggcgctgga
     3181 cctcaactcc tcctacagct acctcctgtg gtgccgcgac ttcgccgcca cctccgccgt
     3241 cgcccgcgat gcgaccggcg agccagccgc gttcgtcacc ggctacctgc gtcccgaccg
     3301 cccggacacc ctcgtcgtct ggcagatcgc cgtcgacgcg gcgcaccgcg gacggggcct
     3361 ggccgccgca ctcctggacg ggctgaccgc ccgcgccatc gacgaactgg gcgtccgcag
     3421 cgtggagacc accatcacgc ccgacaacga cgcctccaac cggctgttcg cctccttcgc
     3481 cgcacggcac tcagtgccgg tcaagcgcga ggtgctgttc gacgccgcgc tcttccccga
     3541 acagggccac gagccggaag tcctgcacct cataggcccg ttcgagacgc cgcccggcag
     3601 caaccgctga cccgggccgc gcgcacggcg ccgcccccca gacttctccc tcccgcactc
     3661 atctcccagg agcatgctgt gaccatcacc cagcccgatc tgagcgtctt cgaaaccgtc
     3721 gagtcggagg tgcgcagcta ctgccgtggc tggcccaccg tcttcgaccg cgcgcagggc
     3781 agccgcctga ccgacgagga cggccacacc tacctggact tcttcgccgg cgccggctcg
     3841 ctcaactacg ggcacaacaa cccggtcctc aaacgcgccc tgatcgacta catcgagcgg
     3901 gacggcatca cccacgggct ggacatgtcc accacggcca aacgggcctt cctggagtcg
     3961 ttccagaaca tcatcctgcg gccgcgcgac ctgccgtaca aggtcatgtt cccgggcccg
     4021 acgggcgcca actcggtcga ggccgcgctc aaactggccc gcaaggtcaa ggggcgggag
     4081 tcgatcgtct ccttcaccaa cgccttccac ggcatgtcgc tgggtgcgct cgcggtcacc
     4141 ggcaactcca tgaagcgggc cggcgccggc atcccgctgg tgcacggcac cccgatgccg
     4201 ttcgacaact acctggacgg ccagacaccg gacttcctgt ggttcgagcg gctgctggag
     4261 gaccagggct cgggcctgaa caagccggcc gcggtgatcg tggagaccgt gcagggcgag
     4321 ggcggcatca acgtcgcccg cgccgactgg ctgcgcgccc tggccgccct ctgcgagcgc
     4381 caggacatgc tgctgatcgt cgacgacatc cagatgggct gcggccgcac cggcgccttc
     4441 ttctccttcg aggaggcggg catcgtgccg gacatcgtca ccgtctccaa gtccatcagc
     4501 ggctacggac tcccgctggc cctgaccctg ttcaagccgg agctggacat ctgggagccc
     4561 ggcgagcaca acggcacctt ccgcggcaac aacccggcct tcgtcaccgc ggccgccgcc
     4621 ctggacacct actgggccga cggccagatg gagaagcaga cgctgggccg cggcgagatc
     4681 gtcgaggggc acctgaaggc catcgtcgcc gagcacccgg gcgccttcgc cgagtaccgc
     4741 ggccgcggtc tggtctgggg cctggagtgc accgacaagg agctcgccaa caagatcgcc
     4801 aagcgcgcct tcgagctggg gctcctcatc gagacctccg gcccgcagag cgaggtcgtc
     4861 aaactgctgc ccgcactgac gaccaccccc gaggaactgg acgagggcct gcggatcctc
     4921 gcccgcgcgg tgcgcgactg cgcctgaccg cacccgtcgt ccgacgcctc accgttccac
     4981 agaaaggcat ccaacgcacc atgatcgtcc gctcgttcaa ggacatcgaa ggcaccgacc
     5041 gccacgtcaa ggccaagtcc ggcacgtggg agagcaagcg catcgtgctc gccaaggagc
     5101 gggtcggctt ctcgctccac gagaccgtcc tctacgccgg caccgagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtggtctgca cggagggcgt ggccgagctg accaatgacg
     5221 agaccggcga gaagcacctc atcacgcccg gcgtgatgta cctgctcaac ggccacgaga
     5281 agcacaccat gcggatcaag gaggacttcc gctgcctgtg cgtcttcaac ccgccggtca
     5341 ccggtcgtga ggaccacgac gagaacggcg tctacccgct gctcaccgag cccgaacccg
     5401 aggcgagctg agcgcggaca ccggaccgtc caccgtccgg cgtcaccgtg acgcagtgag
     5461 attccgtacg agaggagagg aaggcaccac catgaccacc gcacccgagc gcaccgccga
     5521 cctgtacccg acccgtggga cctcagaggt catcaccccg cggcaggacc cggtggtgtg
     5581 gtcgcagccc ggcgcggagg gccccttcgc cgcgtccgag ctgagcgact tcgaccgtga
     5641 cggcttcttc gccatgccgg agctgctcac cgcgggcgag gtcgcggtgt accgcgccga
     5701 gctggaccgg ctggtgaacg acccggcgac gcgcgcggac ccgcgctcga tcgtcgagcc
     5761 gcagtcgcag gacgtccgct cggtcttcga agtgcaccgg atcagcgacg tgttcgcacg
     5821 cctggtctcc gatccgcgcg tggtgggccg ggcccgccag atcctcggct cggacgtcta
     5881 cgtccaccag tcgcggatca acgtcaagcc cgggttcggt gcctccggct tctactggca
     5941 ctcggacttc gagacctggc acgccgagga cggcctgccg aacatgcgca cggtgtcggt
     6001 gtcgatcgcg ctgaccgaga actacgacac caacggcggc ttgatgatca tgccggggtc
     6061 gcaccggcac ttcgtcgggt gcgagggcgc caccccgaag gacaactaca agaagtcgct
     6121 gcagatgcag gacgcgggca cgccgtccga cgggacgctg acgacgctgg cggaccggca
     6181 cggcatccgg ctgttcaccg gcaaggccgg ctcggcgacg tggttcgact gcaacgccat
     6241 gcacggctcc ggcgacaaca tcacgcccta cccgcgcagc aacgtcttta tcgtcttcaa
     6301 cagcatggag aacaccgccg tggagccgtt cgcggcgccg gtcaggcgcc cggagtacat
     6361 cggggcgcgg gccccgttcg caccggtcaa gtaggaaacc gtcggcccgc ccgccccggc
     6421 gtcgaccggc ggaacgcgga cggccgccgc acagtccctc gtggcactgt gcggcggccg
     6481 tccgcgttcc gccggtcgac gcgaataccg acctaacgtg acgtcagttg tcgtccaggg
     6541 acgggtagtc caggtagccg tggtcgtcgc cgccgtagaa gctggcccgc tccgggacgt
     6601 tgaacgggcc gccggccgcc aggcggcggg gcaggtcggg gttggcgatg aacagcgcac
     6661 cgtacgccag cagatcggcg tcgccctgct cgacgagcga cagctcctcg gggccggtgg
     6721 ggcgaccgac ggtggccggg ttgagcagga acgggccgtc gaaccgcttc cgcagccggg
     6781 ccagcagctg gaggtcgtgg acgtcgcagg tgtgcaggta ggccaggccg agcggggaga
     6841 cggcgtccag cagcgcggtg taggtggcct ccggctcggg ctcggcgatg tcgttgaacg
     6901 ggttgccggg cgagagccgc agaccggtgc ggtgcgcgcc gaccgcctcc gcgaccgcct
     6961 tgaccacctc gatggcgaac cgggcgcggg ccgcgtcgga gccgccccac gcgtcggtgc
     7021 gcaggttggc gttgggagcc aggaactggt ggacgaggta gccgttggcg ccgtggatct
     7081 ccaccccgtc gaagccggcc tcgatggcgt tgcgggccgc ggccgcgaac ccgtcgacgg
     7141 tcgcgcggat ctcctcgtcg gtcagctcgc gcggggtgac gaagtccgtc ggaccgttct
     7201 gggtgaagcc ctggccggcc gcccgcaccg cggacgggcc gaccggctcc agtccgtcgg
     7261 gcagcagcac cgggtggccg atccggccgc cgtgccacag ctgggcgaag atccgaccgc
     7321 cggccgcgtg gacggagtcg gtgacccggc gccagcccgc gacctgctcg gcggtgtgca
     7381 gacccggcgt gagggggtag ccgcggccga ccacctcggt ctgggtggcc tcggtgacga
     7441 tcaggccggc cgacgcccgc tgggtgtagt acgtgaccat cgagggggtg ggcacgcccg
     7501 cctcggtggc gcggttgcgg gtcatcggcg ccatcacgat gcggttggcg agcgggatgc
     7561 cggcgagttc tatggggtcg aacgcggtgg tcactggcgg cctctcagct ggggggacac
     7621 ggggcgccaa ctcacctgtt cgagctgccg ccttggccat tagatcagcc cccgcatccc
     7681 cggccacgcc cccctggcct gcgaaaacac cacggcaacg gggggcagtc gaggcgttac
     7741 ggaaccaaca gttcgagcgc ccggtccacg tccgcgcggg agttgtagag gtggaacgcc
     7801 gcccggagca gcccgccgcg gaccgcgacc cgcaccccgg ccgcggccag ccgcggttcg
     7861 gcgtccgcca gcccgggggt ggagacgatc gcggagccgg gcgcggcccg cggggtgaag
     7921 ccggccgcca ccagccccgc ccggtactgc tcggcgagcg ccacgtcgtg cgcgtggatg
     7981 gttgccagcc cgatctccgc gagcagcccg agggagtgct cggcgcccac gtaggcgtag
     8041 ttggcgtgcg gttcgtcgta gcggcgggcg gtcggcgccg gccggtcgac ggggccgtag
     8101 ttcgactccc ccgggtcctc gcccgcgacc catccggcgt gcgtcggcac cggccatccg
     8161 gggccgccca actcgccgcg ctcgccgccg aagacggtga acgtcgtccc cttcggacac
     8221 agcagccact tgtaggcccc gcacaccgcg tagtcgaagt cggcgagcgg caccggcagc
     8281 cagcccgccg cctgcgtgac gtcgacgtag gtcagcgccc cgtgcgcccg ggcggcgtcc
     8341 cggacggcgg ccaggtcggc gatccggccg tcctgggggt gcaccgcgct gagcgcgacc
     8401 agtgcggtcc gggggcgcac cgcctcggcg agcgcctccc gcggcaccgc ccgcaccgtg
     8461 cagccgccgt gcgccatcag cgggttcacc agcgagctga agtccccctc ggcgaccagc
     8521 acttcgctgc cggccggcaa cgacgcggcg acgaacgccg agcggaccgc gaccgaactg
     8581 cccaccgcca cccgctccgc cggcaccccc atcagccgcg cgaacacccc gcgcgcccgg
     8641 tccgccgtcc cgaagtagtc gcgccccatc gtcccgtgcg acgccgactc cgccagcgcg
     8701 tcccgcaccg cggcggcggt ccgcgcgggc agcagcccgc tcgacgccga gttcaggtag
     8761 accgtctcgg gcgcgaactc gtccccgccc agtgccgtgt gtcgctgcat ggcccccact
     8821 ctggtcggta acggctccgg cgtccacggg ggcgccggac ccgccgcggc ggttccggcc
     8881 ggctcacgcc cccggaacgg cgcagccccc gtccccgcag acgtccgcgt cgccggcggc
     8941 cagcacctgg acggcggagt cggcgtgcgc ccgctgcagc gcctggtgga agacgtccac
     9001 cggctgggcg cccgagatgc cgtagcggcg gtcgatgacg aagaagggga cgccggtggc
     9061 gcccagctcg gcggcggtgc gctcgtcggt ccggacctcg gcggcgtagg cgtccggatc
     9121 ggcgaggacc cgggcggcct cgtcggcggg caggccggcc tcgaccgcga tcccggtcag
     9181 caccgccggg tcgccgagct gtcgggcctc ggcgaagttg gcgtggtaca gggccgtgag
     9241 cagggcgtcc tgcaccccgt gggccttggc caggtggagc aggcggtgca ggtcgaaggt
     9301 gttgccgtgg atgcggtccg agcggtagcc gagcccctca ccggcggcgg cctcggcgac
     9361 ccgggactcc atctcctcgg cctgggcccg gctgacgccg tacttcgacg ccagcatgtc
     9421 gaggatcggc acgtcggtgg cgggcggggc gtcccggtcc agctcgaagg aacggtggac
     9481 gacctcgacg tccgcccggt gggcgaaggc cgccaggccg gcctcgaacc gggccttgcc
     9541 gatgtagcac caggggcagg cgatgtccga ccagatttcg acgcgcatgg ggggtgactt
     9601 tcctcggggg gaccggtcgg cgaccggtcg gggtacgaag cggtgcaacg cggcggcggg
     9661 cgcggttatt ccggaggtgg gcggcgcgcg ggagttcacg gtgccctcgg cggtaccgtc
     9721 ccggtatggg cacgccagca ccgcacggca ccgacgggtt ctgggagacg accggggccg
     9781 ccaagacctt cacccacccg atcgatcccg cgctgctggc ggagttcgtg ccgcgcaccg
     9841 cgcgggtgct ggactacggc tgcgggtacg gacggctcac cgcggagctg gtggggctgg
     9901 ggtacggggc ggtgcgcggg gtggaccggt cggcggcgct gatcgcccgg gggcggcagg
     9961 cgcatccggg gctggcgctg ctgcggtggt cggggttccc gctgccgttc gcggccggca
    10021 gcttcgacgc ggcgctgctg ttcgcggtgc tcacctgcgt ccccgacgac gccgatcagc
    10081 gggcgatcgt cggggagctg gcgcggctgg tgcggccggg cggggtgctg tacgtgagtg
    10141 acgtgccgtt gcaggacgac ccgttcaacc gcgcgcgcta cgagcggttc gcggcacggt
    10201 tcgggacgta cggggtgttc cagacgccgg acggcggggt gttccggcac catccgcccc
    10261 cgtggctgcg ggggctgctg cgggcggccg ggttcgcggt gctgcgggag tgccgcacgg
    10321 tggtgccgac gctggacggc cgcaccgccg agcggctcca gctcatgacg cggcgggagc
    10381 cggcggtggc ggggccagcg ccagggtgag c
//
