LOCUS       NZ_CP104098            10216 bp    DNA     linear   CON 08-NOV-2024
DEFINITION  Streptomyces noursei strain IFO14147 chromosome, complete genome.
ACCESSION   NZ_CP104098
VERSION     NZ_CP104098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Transcriptome and Metabolome Analysis Revealing the Improved
            epsilon-Poly-l-Lysine Production Induced by a Microbial Call from
            Botrytis cinerea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9223378)
  AUTHORS   Zeng,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) College of life science, Huaibei Normal
            University, Dongshan road 100#, Huaibei, Anhui 235000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PGCGAP v. 1.0.34
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025163575.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 12:09:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,195
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,883
            CDSs (with protein)               :: 7,883
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 93 of 218
            Pseudo Genes (incomplete)         :: 149 of 218
            Pseudo Genes (internal stop)      :: 11 of 218
            Pseudo Genes (multiple problems)  :: 33 of 218
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:04:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1401952
            Orig. end                         :: 1412168
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    289..298
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1685..3964)
                     /locus_tag="N1H47_RS06115"
                     /old_locus_tag="N1H47_06115"
     CDS             complement(1685..3964)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="N1H47_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06115"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_020930631.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.9e-37, bitscore:
                     125.3, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPDASAAPPQHLPPAGRGRHRPEGAPPARRRRPPAAAPKAKKA
                     VRTPTVLQMEAVECGAASLAMVLAHYGRHIPLEELRIACGVSRDGSRASNLLKAARSYG
                     LSAKGMQMEPAALADVAAPAILFWEFNHYVVYDGPGRRLGRRGVHINDPDKGRRFVPVE
                     DFDTSFTGVVLVFEPTEAFRKGGRRPSVLSALPARLRGTTGTLLAALLASLLLVGVGAA
                     LPALSRTYIDMFLIGNQTSLLGPLFAAMTAMVVLTAVLTGLQQANLLRGRIISSTLSSA
                     RFLRHLLRLPVTFFAQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYAVLLWT
                     YDPQLTVIGVLIALLNVVAMRIVIRLRATHTQKLRADTARLTNTSYTGLQLIETMKATG
                     GENGYFRRWAGQHAATLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHIS
                     IGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADAHYSRPDADAGT
                     RRLKGHVTLEDVTFGYSPLDAPLLTGFSLAVGPGQQVALVGGSGSGKSTVSRLISGLYA
                     PWEGTIRIDGQRLADIPRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAAL
                     QDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALD
                     AGTEAVIMDNLRRRGCACVIIAHRLSTVRDSDEIVVLDHGAVVERGRHAELVAAGGAYA
                     ELVKEH"
     gene            complement(3961..4767)
                     /locus_tag="N1H47_RS06120"
                     /old_locus_tag="N1H47_06120"
     CDS             complement(3961..4767)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540972.1"
                     /locus_tag="N1H47_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06120"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_016576072.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLTVTVLVMIAAAVW
                     AVTGTVSATVSAPGILTHGHGSYVLQSPVAGQVTEVLAEEGKQVTAGAPLVRIRTAQGT
                     TTVVRALAAGRLTNLVATIGAVVTTGADLAAVERTTGTRDPLTATLYVPAENAATLPVG
                     APVDLTVAAAPTQRFGVLRGHVAAVGRAAQSRQRISAFLGSTQLGEQFSRAGQPIAVLV
                     RLDPARTASGYAWSTSDGPPFALDSMTQATAAVHLAAQHPIDWLLS"
     misc_feature    4732..4744
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     misc_feature    4739..4756
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     misc_feature    complement(4906..4920)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            complement(5001..5216)
                     /locus_tag="N1H47_RS06125"
                     /old_locus_tag="N1H47_06125"
     CDS             complement(5001..5216)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="N1H47_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06125"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_016576071.1"
                     /sec_met_domain="strepbact (E-value: 1.7e-23, bitscore:
                     81.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVVNTVTTTVGSVAPVTGVL
                     NTATGLVQQTTGLDVQGVAGNLTAGL"
     misc_feature    complement(5234..5248)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     misc_feature    5235..5248
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    5235..5249
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.89"
                     /tool="antismash"
     misc_feature    5236..5249
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.77"
                     /tool="antismash"
     misc_feature    complement(5584..5602)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    5607..5616
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    5686..5703
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.91"
                     /tool="antismash"
     misc_feature    5695..5711
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(5705..5719)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.67"
                     /tool="antismash"
     gene            5889..6929
                     /locus_tag="N1H47_RS06130"
                     /old_locus_tag="N1H47_06130"
     CDS             5889..6929
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676609.1"
                     /locus_tag="N1H47_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06130"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_037635476.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQGAVTYRVQAFRTAASVLTALPADALRER
                     AGNGTLGRLKGLGPKTTRVVEEALAGERPAYLTRLEEEAGGPLVEGEAGRALRRALRGD
                     CHLHSDWSDGGSPVEAMARTARDLGHAWCALTDHSPRLTVARGLTAERLRQQLAVVAEV
                     NARLAPFRLLTGIECDILDHGSLDQEPELLAELDVVVASVHSKLRMDAGPMTRRLLAAV
                     RNPLVDVLGHCTGRQLTGKLRPESEFDAAEVFAACAEHRTAVEINCRPDRLDPPRRLLR
                     QALDAGAFFAVDSDAHAPGQLDWQIYGCARAEECGVPADRIINTWTARQLTAWTRTGRP
                     PRGAGT"
     gene            complement(6954..7862)
                     /locus_tag="N1H47_RS06135"
                     /old_locus_tag="N1H47_06135"
     CDS             complement(6954..7862)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="N1H47_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06135"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_020930629.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTAAGDILLGHANAVLAQLERAEAELAAYDEGEAGTVTVAAFATGIA
                     LVLAPALAELAGSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPDADDPRLTR
                     VPLYAEPFDAVLPPGHPLAGVAAVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFRPR
                     LTHSSDDFRAVVALASAGVGVALVPRSALRGMDLGGVVVRPVDGVAPTRRVFAAVRRGA
                     EDHPLFRPVLTALRATAADLG"
     misc_feature    complement(7827..7843)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            complement(7920..9113)
                     /locus_tag="N1H47_RS06140"
                     /old_locus_tag="N1H47_06140"
     CDS             complement(7920..9113)
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (45-388). E-value: 2e-72.
                     Score: 236.2. Matches aSDomain:
                     nrpspksdomains_N1H47_RS06140_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999157.1"
                     /locus_tag="N1H47_RS06140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06140"
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_016577364.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.9e-70, bitscore:
                     236.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDAVRDDLRTALDEIRAAGLHKPERVIGTPQSATVAVTAGGRPG
                     EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQEVHKELERRISHFLG
                     QEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLSKARRLRYANRDLAD
                     LERQLKEAQGARRTLVVTDGVFSMDGYVAPLREICDLADRYGAMVMVDDSHAVGFVGAG
                     GRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQRSRPYLFSNSLAPV
                     IAAASLKVLDLLESADDLRERLHANTALFRSRMTEEGFAILPGAHPIAPVMIGDAAEAA
                     RMAELLLERGVYVIGFSYPVVPQGEARIRVQLSAAHSTEDVHRAVDAFVAARAALGS"
     aSDomain        complement(7950..8978)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_N1H47_RS06140_Aminotran_1_2.1"
                     /evalue="2.00E-72"
                     /label="N1H47_RS06140_Aminotran_1_2.1"
                     /locus_tag="N1H47_RS06140"
                     /protein_end="388"
                     /protein_start="45"
                     /score="236.2"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQE
                     VHKELERRISHFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLS
                     KARRLRYANRDLADLERQLKEAQGARRTLVVTDGVFSMDGYVAPLREICDLADRYGAMV
                     MVDDSHAVGFVGAGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLESADDLRERLHANTALFRSRMTEEGFAILPGAH
                     PIAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPQGEARIRVQLSAAHSTEDVHRAV
                     DAF"
     gene            complement(9186..10214)
                     /gene="tdh"
                     /locus_tag="N1H47_RS06145"
                     /old_locus_tag="N1H47_06145"
     CDS             complement(9186..10214)
                     /EC_number="1.1.1.103"
                     /GO_function="GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tdh"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922312.1"
                     /locus_tag="N1H47_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="N1H47_06145"
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="WP_016577365.1"
                     /sec_met_domain="ADH_N (E-value: 7e-30, bitscore: 102.0,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 6.3e-23, bitscore:
                     80.1, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKALVKQKAEPGLWLMDVPEPEVGPGDVLIKVLRTGICGTDLHIR
                     AWDGWAQQAVSAPLTVGHEFVGEVVGTGRDVADIKAGDLVSGEGHLVCGSCRNCLAGRR
                     HLCRATVGLGVGRDGAFAEYVALPAANVWVHRVPVDLDIAAVFDPFGNAVHTALSFPLV
                     GEDVLITGAGPIGIMAAAVARHAGARNVVITDVSEHRLDLARKVGVSLALNVAATSIEA
                     GQRELGLREGFDVGLEMSGRPEALRGMIGNMTHGGRIAILGLPSEEFPVDWSRIVTSMI
                     TIKGIYGREMFETWYAMSVLLEGGLDLAPVITGRYPYQDFDAAFADAASGQGGKVILDW
                     SA"
ORIGIN
        1 gatgacgagc agcacggcga acgcggcgag cgccagcggg acgctgtaga cgagcagcag
       61 cgcgaggttg accgcgccga ccgtgccggc ctgcacggcg accggcccga ccccggccag
      121 cacccggcgg atggcgctga tgcccatcgc ggcgctggcc agttcgccgg ttgagcgggc
      181 ggcgaagaac gaggtgggca ggcgcagcag ccggtcccag acggcgggct gcagcacgct
      241 ctcgatgcgg ccttcgatgc gcagcacggt gaggttctgc aacagggtga aggcggccgc
      301 gacgatgccg gcgatcatca cggcgagcgc gacctggacg atcgggccgc gggcggcggc
      361 cggcacgaag acgccgagca ccttgccggt ggcgatcggg acgagggcgc cgagggcgac
      421 cgtcaccagc ccgctgaggg cgaggttgcg caggtccgtg cggttcccgc ggaggctgaa
      481 gcgcatcagc cgccacagcg tcagccggcg gtccggcagc ggccggtaga acatcaccgc
      541 gcgcggttcg aactcctcgg cgttgtcctt gccgatccgg atccgcagcc cggacgtcgg
      601 gttgaccgct tcgtaccggc cgcgccgcca cagcagggcc accggcgcgc cggacttggc
      661 gcggtggccc accaggggcc cgacgtcggt gcgccaccag gggccgtcga ggcggaccgc
      721 gcgggtgcgg atccgggagg ccagggcgat gcgttcgacc gggctgatcc ggtcgccggc
      781 cgcggcgccc ttgggcggct cggtgagcgg gatgccggcg gcctgggcga cctgccggca
      841 gaccgcgaag gtcaggtcgt cgccggcccg gtcgcggccc gcgccggcac gcccggagcg
      901 gtccttgccg atcgaggcca gcagcgcccg gtcggcgcgt tcccggacgg cctcgccggc
      961 cttgatgccg gccgccgcac ggtcctcgtg ggcgcgttcg agctcttcga tccagcggtc
     1021 gacggcggac agcagccggt actgctggtt gaccatcccc tgccacagcg cgccgtcgac
     1081 gagcaggtcg gcggcggcct cggcgctgta ggaggcgccg tactggacgc tgccgggcgg
     1141 gaccggcatc cacagcacgt cgtcgtcggc gaccgcctcg tcggtgggcc ggcccgcgag
     1201 cggcgcctcg aacagcacgc ccagtccgcg ggcggtgccg agcgcgaagg cgtgctccag
     1261 ggggctggcg gccggcgccc cgtgggcgtc ggcgtagccg tacgccccgg gatgcgcgcc
     1321 ggggtcggcg tgacctccgt gctctgggcg gggcagttcc cgcagcgcga tccggcgcag
     1381 tccgcagtgt tgggacgggc ggccgacgag ggtgtgctgg gcgccctcga cggggccgag
     1441 cagcagggtg ccggtttcga ggcggccgag gaagtgccag gggccctcgt gggtggcgtc
     1501 gaccgcgaac aggtcgaggg tgccgccggt gaccagccac aggacgtgcg ggccttcgag
     1561 ggggacgttg cgcaggcccg tgtggtccac gggggtgccg aggccgccca gggcgtgggt
     1621 gaccgcgtcg gcggccggtg ccgtggcggg tgccgcgtcc ggcggcgggg gtacggatgc
     1681 cacgtcagtg ctccttgacc agctcggcgt aggcgccccc ggcggcgacc agttccgcgt
     1741 gccggccgcg ttcgacgacc gcgccgtggt cgaggaccac gatctcgtcg ctgtcgcgga
     1801 cggtgctcag ccggtgggcg atgatgacgc aggcacagcc ccggcgccgc aggttgtcca
     1861 tgatgaccgc ctcggtgccg gcgtccaggg cgctggtgac ctcgtcgagg acgaggatgc
     1921 tggggcgccg gaccagcgcc cgggcgatct ccaggcgctg ccgctgaccg ccggagaagt
     1981 tgcggccgtc ctgctcgacg cggctgtgga tgccgtcggg gcggcgggct atgacgtcgt
     2041 cgtagagggc ggcgtcctgg agggcggcga tcaccgcgtc gtccgggatg gaggggtccc
     2101 acagcgcgac gttgtcccgg acggtgcctt cgaagaggaa gacgtcctgg tcgacgaagg
     2161 agaccgaggc ggccagcgcg ctgcgcggga tgtcggcgag gcgctgtccg tcgatccgga
     2221 tggtgccttc ccaaggggcg tagaggccgg agatcagtcg ggagacggtg gacttgccgc
     2281 tgcccgagcc gccgaccagg gcgacctgct ggccggggcc gacggccagc gagaagccgg
     2341 tcagcagcgg ggcgtcgagc gggctgtagc cgaaggtgac gtcctccagg gtgacgtgcc
     2401 ccttgaggcg gcgggtgccg gcgtccgcgt ccgggcggga gtagtgggcg tcggcgggga
     2461 agctctcgac gtccttgagg cgggcgacgt cggcggcgaa gtcctggatc cggccggcga
     2521 cgccgttgag ccgggtgatc ggggcggtga agcgggtcac cagggcctgg aaggcgacca
     2581 acaggccgat cgagatgtgg ccttcgacgg cgcgcaggcc gccgatccac aggatcagcg
     2641 cgctgttgag ggtggcgagg gtgggggcga cgaccgccag ccaggcgctg gggacgccga
     2701 gccgctgctg ttcctccagg gtggcggcgt gctgcccggc ccagcggcgg aagtagccgt
     2761 tctcgccgcc ggtggccttc atcgtctcga tgagctggag gccggtgtag gaggtgttgg
     2821 tcaggcgggc ggtgtcggcg cgcagcttct gggtgtgggt ggcgcgcagc cggatcacga
     2881 tccgcatggc gacgacgttg agcagcgcga tgagcacgcc gatgacggtg agttgggggt
     2941 cgtaggtcca cagcagcacg gcgtagagga cgacgaccac gccgtcgacg gccgccgcgg
     3001 ccaggtcgcg ggcgagggtc tcggcgaccg cgtcgttgga ctgtagtcgc tggacgaggt
     3061 cggcgggcga gcgctgggcg aagaaggtga ccggcaggcg cagcaggtgg cgcaggaagc
     3121 gggcgctgct gagggtggag gagatgatcc ggccgcggag caggttggcc tgttggaggc
     3181 cggtcaggac ggcggtgagc accaccatcg cggtcatcgc ggcgaacagc ggccccagca
     3241 gcgaggtctg gttgccgatc aggaacatgt cgatgtacgt gcggctgagc gcggggagcg
     3301 cggcgccgac gccgaccagc agcaggctgg cgagcagcgc cgccagcagg gtcccggtgg
     3361 tgccgcgcag ccgggccggc agggcgctga gcacgctggg ccgacgaccg cccttgcgga
     3421 acgcctcggt gggttcgaag accagcacca cgccggtgaa gctggtgtcg aagtcctcca
     3481 ccggcacgaa gcggcggccc ttgtcggggt cgttgatgtg gacgccgcgg cggccgaggc
     3541 ggcggcccgg gccgtcgtag acgacgtagt ggttgaactc ccagaagagg atggccggtg
     3601 ccgcgacgtc ggcgagggcg gccggttcca tctgcatgcc cttggccgac aggccgtaac
     3661 tgcgggcggc cttgaggagg ttgctggcgc gggagccgtc gcgggagacg ccacaggcga
     3721 tccgcagttc ctccagcggg atgtgtcgtc cgtagtgggc caggaccatc gccagggagg
     3781 cggcgccgca ctccaccgct tccatctgga gcaccgtggg ggtgcgcacg gccttcttcg
     3841 ccttgggggc ggcggccggt ggtctgcggc ggcgggcggg tggggcgccc tcgggccggt
     3901 gccgaccgcg gccggcgggc gggaggtgct gcgggggcgc ggcggaggcg tcgggggcgg
     3961 tcacgagagc agccaatcga tcgggtgctg cgcggccagg tggacggcgg cggtggcctg
     4021 ggtcatcgag tcgagggcga acggcggccc gtcggaggtg gaccaggcgt agccggaggc
     4081 ggtgcgcgcg gggtcgagcc ggaccagcac cgcgatcggc tggccggcac gggagaactg
     4141 ctcgccgagt tgggtgctgc cgaggaacgc ggagatccgc tggcggctct gggcggcccg
     4201 gccgacggcc gcgacgtggc cgcgcagcac cccgaaccgc tgggtggggg cggcggcgac
     4261 ggtgaggtcg acgggggcgc cgaccggaag ggtggcggcg ttctcggccg ggacgtagag
     4321 ggtggcggtg agcgggtcgc gggtgccggt ggtgcgttcc actgcggcga ggtcggcgcc
     4381 ggtggtgacg accgcgccga tggtggcgac gagattggtc aaccggccgg cggcgagcgc
     4441 ccggacgacg gtggtggtgc cttgggcggt gcgtatccgc accagcggcg cgccggcggt
     4501 gacctgtttg ccctcctcgg cgaggacctc ggtgacctgt ccggcgaccg ggctctggag
     4561 cacgtaactg ccgtggccgt gggtgaggat gccgggggcg ctgacggtgg cggagacggt
     4621 gccggtgacc gcccagacgg cggcggcgat catgacgagg acggtgacgg tcagcaccag
     4681 ccagccctgc gggcgggcga agcgcaccgg caggtccagt tcctccggcg attgcagctt
     4741 ggaaagggcc tgttgacgga actgcacgac actcttccct caactgcagc ggctggtacg
     4801 ggggtacaga cgcgcgccgg gggcgcgcgg ttccaactcc ggcggcctcc ggtcgcggac
     4861 agccgggtgc cccggaaccc ggtcggaacc gggttgtccg gggcactcga cacggcagat
     4921 cggcagggag ggggcgccga ttgccgtcgc gaccgttgga tgccggctgt gccgcgcggc
     4981 ggcacagggt cggctccggg tcagagaccg gcggtcaggt tacccgcaac gccctggacg
     5041 tccaggccgg tggtctgctg gaccaggccg gtagcggtgt tgaggacacc ggtgaccggg
     5101 gcgacggagc cgacggtggt ggtgacggtg ttcacgacac cgccgacgag gccaccggac
     5161 acgttgtcca ggtcggcgtc ggaaatttcc tgggtctgca cctgggcgtt gttcatgatg
     5221 tttcccttcg gatcaatatc aagaaaaacc gcgtacgggt ctccggtggg tgccccgggc
     5281 gcggccacgg tagtccgggt cccgatatcg ggaaaccccg gtgactgccg cggtgcgatg
     5341 gatcaaagca tgcgggcggg ttgcgctgct aatcggatga gcggcgatcc gggcatatcc
     5401 accccgcgaa attgccggtc gtgtgcgcgc atccatggca tggtcgaagc atcttcacat
     5461 cttctccggt ttccattccg ctgaaccttc gttgccgccc ctgcggaatg ggacattccg
     5521 gatgcaagtc gccgagcggg ccgacgctgc acgcacgcga aaccgcggcc cgtgtgccgg
     5581 ctgcgcattc ggtgtgcaga tttcctgaag gcggcccgac ttaacgcccg actagcgccc
     5641 ggcggcgacg agaagtgcgc ttcgcgtttc cgccgcacga cctggatgtt gcacaggttt
     5701 taaccgaaca tgattgccct cgacttccac gtcactaatg accggaatca tcacggctat
     5761 cgccccgtca ttccgatgga aacggatcga gcatccagag aacggacata ttcgcataac
     5821 tgcggtgcac gccgttcgcc ggaccggccg cggtggcagg cgccccccgc ccggtgcacg
     5881 gtgggtacgt ggatcccgtg gcggcgctgg agcggatcgc gttcctgttg gagcgacagg
     5941 gggcggtgac gtaccgggtg caggcgttcc gcaccgcggc gtcggtgctg accgcactgc
     6001 ccgcggacgc cctgcgcgag cgggccggca acggcacgct ggggcggctg aagggactgg
     6061 gccccaagac gacccgggtc gtcgaggagg cgctcgccgg cgagcggccc gcctatctga
     6121 cgcgcctgga ggaggaggcc ggcggcccgt tggtcgaggg ggaggcgggt cgggccctgc
     6181 gccgggccct gcgcggcgac tgccacctgc actcggactg gtcggacggc ggcagccccg
     6241 tcgaggcgat ggcccgcacc gcccgcgacc tcggccacgc ctggtgcgcg ctgaccgacc
     6301 actccccccg gctcaccgtg gcccgcggac tgaccgccga gcggctgcgg cagcagttgg
     6361 ccgtggtggc cgaggtcaat gcccggctgg cgccgttccg actgctgacc gggatcgagt
     6421 gcgacatcct cgaccacggc tccctcgacc aggaaccgga gctcctggcc gagctcgacg
     6481 tcgtggtcgc ctcggtgcac tcgaaactgc ggatggacgc cgggccgatg acgcggcggc
     6541 tgctggccgc ggtccgcaac ccgttggtgg acgtgctcgg ccactgcacc gggcggcagc
     6601 tcaccggcaa gctgcgcccg gagtcggagt tcgacgccgc ggaggtcttc gcggcgtgcg
     6661 ccgagcaccg gaccgcggtg gagatcaact gccgcccgga ccgcctcgac ccgccgcgcc
     6721 gcctgctccg tcaggccctg gacgccggcg ccttcttcgc cgtcgacagc gacgcgcacg
     6781 cccccggcca gttggactgg cagatctacg gctgcgcccg cgccgaggag tgcggcgtcc
     6841 ccgccgaccg gatcatcaac acctggacgg cccggcaact gacggcctgg acccggaccg
     6901 gccgcccgcc gcgcggcgcc ggcacctgac gcccgcccca ccgccccggc cgctcacccc
     6961 aggtcggccg ccgtcgcgcg cagggccgtc agcaccgggc ggaacagcgg gtggtcctcc
     7021 gcgccccgcc ggaccgcggc gaagacccgg cgggtgggcg ccaccccgtc gaccggccgc
     7081 accacgacac ccccgaggtc catcccgcgc agcgccgagc ggggcaccag ggccaccccc
     7141 acccccgccg aggccagcgc caccaccgcc cggaagtcgt ccgaggagtg cgtcaggcgc
     7201 ggccggaagc cggcgtactc gcaggccagt acgaccacgt catgacacgg attgccggaa
     7261 taggggccga tccaggggtc cttggcgagc ccggccaacg gcaccgccgc caccccggcc
     7321 aacgggtggc ccggcggcag caccgcgtcg aacggctcgg cgtagagcgg gacgcgggtc
     7381 agccgggggt cgtccgcgtc cggcgcgccc cggtactcga cggccaccgc cacgtcgacc
     7441 tgccggtcca gcaccatcag caggctggcg tcgccctccg cgtcctggac ccggacacgg
     7501 atgccgggcg cggacccggc cagttcggcg agggccgggg cgagcaccag cgcgatgccg
     7561 gtcgcgaacg ccgccaccgt cacggtgccc gcctcgccct cgtcgtacgc ggccagctcc
     7621 gcctcggccc gctccagctg ggcgaggacg gcgttggcgt ggccgagcag gatgtcgccc
     7681 gcggcggtca gccggacgcc gcgggcgccg cgctccacta ggcggtggcc ggtctcctgt
     7741 tccagcgcgg cgagctgctg ggagaccgcc gagggcgtga ggtacagcgc ggcggccgcg
     7801 gcggtcaccg tgcggtggtc ggccagcgca cggaggatgt gcagccgacg tgcttcgatc
     7861 atggatccag tttcgcaggg ttcggccgga tcgtgcccgg ccgcctccgg tcgggccggt
     7921 cagctcccca gcgccgcgcg ggccgccacg aaggcgtcca ccgcgcggtg cacgtcctcg
     7981 gtggagtggg cggccgacag ctggacgcgg atccgggcct cgccctgggg caccaccggg
     8041 tacgagaacc cgatgacgta caccccgcgc tccagcagca gttcggccat ccgggccgcc
     8101 tcggccgcgt ccccgatcat cacgggggcg atcgggtgcg cgccgggcag gatcgcgaag
     8161 ccctcctcgg tcatccgcga gcggaacagc gcggtgttgg cgtgcaggcg ttcgcgcagg
     8221 tcgtcggcgg attccaggag gtccaggacc ttgagcgagg cggccgcgat caccggcgcg
     8281 agcgagttgg agaagaggta ggggcgggag cgctggcgga gcagcgcgac gatctcggcg
     8341 cgcgccgcga cgtaaccgcc ggaggcaccg cccagcgcct tgccgagcgt gccggtgatg
     8401 atgtcgaccc ggtccatcac cccgcacagc tccggggtgc cgcggccacc ggccccgacg
     8461 aacccgaccg cgtgcgagtc gtcgaccatg accatcgcgc cgtagcggtc ggccaggtcg
     8521 cagatctccc gcagcggcgc cacgtagccg tccatggaga acacgccgtc ggtgacgacc
     8581 agggtgcgcc gggcgccctg cgcctccttc aactgccgct ccaggtcggc caggtcgcgg
     8641 ttggcgtagc gcaggcggcg ggccttggag agccggatgc cgtcgatgat ggaggcgtgg
     8701 ttgagggcgt cggagatcac cgcgtcctcc gggccgagca gggtctcgaa gacgccgccg
     8761 ttggcgtcga agcaggagga gtacaggatc gtgtcctcct ggccgaggaa gtgggagatc
     8821 cgccgctcca gttccttgtg cacctcctgc gtgccgcaga tgaagcggac cgacgccagc
     8881 ccgtagcccc agcggtcgag ggccccgtgg gcggcggcga tgacctcggg gtggtcggcc
     8941 aggcccaggt agttgttggc gcagaagttg aggacctcgc cggggcggcc gccggcggtg
     9001 accgcgacgg tggcggactg cggggtgccg atcacccgct ccggcttgtg caggccggcg
     9061 gcccggatct cgtcgagtgc ggtgcgcagg tcgtcacgta cggcgtcgaa catgaggggt
     9121 gtctccttgg gggtgcgcgg gtcgggccgg cgggcggcgg cgtcttgcgg agggagcggg
     9181 cggggtcagg cgctccagtc gaggatgacc ttgccgccct ggccgctcgc cgcgtcggcg
     9241 aaggcggcgt cgaagtcctg gtacgggtag cggccggtga tcacgggcgc gaggtcgagg
     9301 ccgccctcca gcagcacgga catcgcgtac caggtctcga acatctcccg gccgtagatg
     9361 cccttgatcg tgatcatgga ggtgacgatc cgggaccagt cgaccgggaa ctcctcggac
     9421 ggcagcccca ggatggcgat ccgaccgccg tgcgtcatgt ttccgatcat gccgcgcagc
     9481 gcctcggggc ggccggacat ctccaggccg acgtcgaagc cctcgcgcag gcccagttcg
     9541 cgctggccgg cctcgatcga ggtggcggcg acgttgaggg cgaggctgac gccgaccttg
     9601 cgggcgaggt ccaggcggtg ctcgctgacg tcggtgatca ccacgttgcg ggcgccggcg
     9661 tgccgggcga ccgccgccgc catgatgccg atcggcccgg cgccggtgat cagcacgtcc
     9721 tcgccgacca gcgggaagga cagcgcggtg tgcacggcgt tgccgaacgg gtcgaagacc
     9781 gcggcgatgt cgaggtcgac ggggacccgg tgcacccaca cgttggcggc gggcagcgcg
     9841 acgtactcgg cgaacgcgcc gtcccgtccg acgcccaggc ccaccgtggc gcggcacagg
     9901 tggcggcggc cggccaggca gttgcggcag ctgccgcaga ccaggtggcc ctcgccgctg
     9961 accaggtcac cggccttgat gtcggccacg tcccggccgg tcccgacgac ctccccgacg
    10021 aactcgtggc cgaccgtcag cggggcgctg acggcctgct gcgcccagcc gtcccaggcg
    10081 cggatgtgca gatcggtgcc gcagatgccg gtgcgcaaca ccttgatcag gacgtcgccc
    10141 gggccgacct ccggctccgg tacgtccatc agccagagtc ccggctccgc cttctgcttg
    10201 accagtgcct tcaccg
//
