LOCUS       NZ_CP102975            20708 bp    DNA     circular CON 10-JUL-2024
DEFINITION  Staphylococcus aureus strain 16CS0209 chromosome, complete genome.
ACCESSION   NZ_CP102975
VERSION     NZ_CP102975.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2769676)
  AUTHORS   Monecke,S., Roberts,M.C., Braun,S.D., Diezel,C., Muller,E.,
            Reinicke,M., Linde,J., Joshi,P.R., Paudel,S., Acharya,M.,
            Chalise,M.K., Fessler,A.T., Hotzel,H., Khanal,L., Koju,N.P.,
            Schwarz,S., Kyes,R.C. and Ehricht,R.
  TITLE     Sequence Analysis of Novel Staphylococcus aureus Lineages from Wild
            and Captive Macaques
  JOURNAL   Int J Mol Sci 23 (19), 11225 (2022)
   PUBMED   36232529
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769676)
  AUTHORS   Braun,S.D., Diezel,C., Reinicke,M., Linde,J. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2022) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024741735.1-RS_2024_07_10
            Annotation Date                   :: 07/10/2024 04:01:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,622
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 40 of 83
            Pseudo Genes (incomplete)         :: 40 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:15:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2025801
            Orig. end                         :: 2046509
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102975.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20708
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="7"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20708
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20708
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10708](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10708
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    985..1001
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(1476..2102)
                     /locus_tag="NW953_RS09890"
                     /old_locus_tag="NW953_09890"
     CDS             complement(1476..2102)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216896.1"
                     /locus_tag="NW953_RS09890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09890"
                     /product="hypothetical protein"
                     /protein_id="WP_000216892.1"
                     /transl_table=11
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNILNSFETISISGSESAH
                     DVDLFLKNKSIAFDRQNLSRTHLVFSQFKSKQILVGYFTISNKPLVFTKRMLDKISNTL
                     KKKLYQKGETHSGNDNLIIQGYLIAQIGKNYSEEALATKSINGNDLLTLAYEKVLEGAN
                     IFGGSYIWIEYEDVDRLREFYRKFGFTEIKDHTSENNLKMAILKI"
     gene            complement(2099..2278)
                     /locus_tag="NW953_RS09895"
                     /old_locus_tag="NW953_09895"
     CDS             complement(2099..2278)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /locus_tag="NW953_RS09895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09895"
                     /product="hypothetical protein"
                     /protein_id="WP_000201397.1"
                     /transl_table=11
                     /translation="MATISITTDYKFTKKSAQKLIDAMEINENNSNVNKTNIKATKIKS
                     TCEIENLLKDYRSN"
     gene            complement(2818..4434)
                     /gene="groL"
                     /locus_tag="NW953_RS09900"
                     /old_locus_tag="NW953_09900"
     CDS             complement(2818..4434)
                     /GO_function="GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /GO_process="GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /locus_tag="NW953_RS09900"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; 60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; 60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09900"
                     /product="chaperonin GroEL"
                     /protein_id="WP_258409284.1"
                     /transl_table=11
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTA
                     PLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLK
                     NVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEDIGRYISEA
                     MEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTD
                     KKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGD
                     RRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSI
                     DARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALN
                     STRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLE
                     GSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAV
                     VASIPEKNNDQPNMGGMPGMM"
     gene            complement(4510..4794)
                     /gene="groES"
                     /locus_tag="NW953_RS09905"
                     /old_locus_tag="NW953_09905"
     CDS             complement(4510..4794)
                     /codon_start=1
                     /gene="groES"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /locus_tag="NW953_RS09905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09905"
                     /product="co-chaperone GroES"
                     /protein_id="WP_000917289.1"
                     /transl_table=11
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGTG
                     RLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            4969..5712
                     /gene="mroQ"
                     /locus_tag="NW953_RS09910"
                     /old_locus_tag="NW953_09910"
     CDS             4969..5712
                     /codon_start=1
                     /gene="mroQ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /locus_tag="NW953_RS09910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09910"
                     /product="CPBP family intramembrane glutamic endopeptidase
                     MroQ"
                     /protein_id="WP_000197638.1"
                     /sec_met_domain="Abi (E-value: 9.5e-17, bitscore: 58.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIYI
                     QLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIVYT
                     QIFGTQQTSPNTERLIVIARKIPLFIFFVSIVGPLLEEYVFRKVIFGELFNAIKGNRIV
                     AFAIATTVSSLIFALAHNDYKFIPIYFGMGVIFSLAYVWTKRLAVPIIIHMLQNGFVVI
                     FQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(5737..7059)
                     /locus_tag="NW953_RS09915"
                     /old_locus_tag="NW953_09915"
     CDS             complement(5737..7059)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /locus_tag="NW953_RS09915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09915"
                     /product="SdrH family protein"
                     /protein_id="WP_258409285.1"
                     /transl_table=11
                     /translation="MSYHWLKKTILSTSILILSSSSLGLATHTVEAKDNLNGEKPTTNL
                     NHNVTSPSVNSEINNNETGTPHESNQTGNEGTNSNSRDANPDSNNVKPDSNNQNPSPEP
                     KPDPNNPNPGPEPKPDPNNPNPGPNPKPDLDKPKPNPEPKPDPDKPKPNPEPKPDPDKP
                     KPNPEPKPDPDKPKPNPEPKPDPDKPKPNPDPKPDPNPNPKPDPNKPNPNPSPNPNQPG
                     DSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGNDFVSQRFLALANGAYKY
                     NPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYFRFQYFNPLKSE
                     RYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKQRSFEPHEKDDFTVVKKQEDNKKSA
                     STAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     misc_feature    complement(7143..7157)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.53"
                     /tool="antismash"
     gene            7256..7882
                     /locus_tag="NW953_RS09920"
                     /old_locus_tag="NW953_09920"
     CDS             7256..7882
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /locus_tag="NW953_RS09920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09920"
                     /product="nitroreductase family protein"
                     /protein_id="WP_000522384.1"
                     /sec_met_domain="PF00881 (E-value: 9.5e-16, bitscore: 55.6,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHAV
                     KHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSYGT
                     ILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYNPLV
                     DEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            8243..9028
                     /locus_tag="NW953_RS09925"
                     /old_locus_tag="NW953_09925"
     CDS             8243..9028
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /locus_tag="NW953_RS09925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09925"
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="WP_000867948.1"
                     /transl_table=11
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNN
                     GYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSGKL
                     INEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGA
                     KIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDL
                     VGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(9631..9765)
                     /locus_tag="NW953_RS09930"
                     /old_locus_tag="NW953_09930"
     CDS             complement(9631..9765)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF011337.0"
                     /locus_tag="NW953_RS09930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09930"
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="WP_001549197.1"
                     /transl_table=11
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK"
     gene            10001..10564
                     /locus_tag="NW953_RS09935"
                     /old_locus_tag="NW953_09935"
     CDS             10001..10564
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105709.1"
                     /locus_tag="NW953_RS09935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09935"
                     /product="accessory gene regulator AgrB"
                     /protein_id="WP_001105709.1"
                     /sec_met_domain="AgrB (E-value: 2.2e-33, bitscore: 112.4,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLIV
                     MYTLAYILNIFIFTLITNISFYLIRRYAHGAHAPSSFWCYIESITLFIVLPLLVLHFHI
                     NETLMMFLALLSVGVVIKYAPAATKKKPIPARLVKQKRYFSIIISTILFIITLFVKEPY
                     TQFIQLGIIIQAITLLPIYYSKED"
     gene            10568..10708
                     /gene="agrD"
                     /locus_tag="NW953_RS09940"
                     /old_locus_tag="NW953_09940"
     CDS             10568..10708
                     /codon_start=1
                     /gene="agrD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735197.1"
                     /locus_tag="NW953_RS09940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09940"
                     /product="cyclic lactone autoinducer peptide AgrD"
                     /protein_id="WP_000735197.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.1e-07, bitscore:
                     29.1, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLLNKVIELLVDFFNSIGYRAAYINCDFLLDEAEVPKELTQLH
                     E"
     gene            10733..12025
                     /gene="agrC"
                     /locus_tag="NW953_RS09945"
                     /old_locus_tag="NW953_09945"
     CDS             10733..12025
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /gene="agrC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837307.1"
                     /locus_tag="NW953_RS09945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09945"
                     /product="quorum-sensing sensor histidine kinase AgrC"
                     /protein_id="WP_000387809.1"
                     /transl_table=11
                     /translation="MEALNDYNYVLFVIVQVSLMFFISAFISGIRYKKSDYIYIIGIVL
                     SSVYFFDKIGSISLVVITIFIIIFLYFKIRLYSVFLVMVTQIILYCANFVYIIIFSYII
                     TISHSVFIVLPIFLVVYVSISYALAYILNRILKRINGTYLSLNKKFLTVITIVIVITFS
                     LLFAYSQIDASDASTIKQYSLLFLGIIILLSILIFIYSQFTLKEMKYKRNQEEIETYYE
                     YTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMTGLRDYFNKNIVPMKDNLQMNALK
                     LNGIENLKVREIKGLLTAKILRAQEMNIPISIEIPDEVTRINLNMIDLSRSIGIILDNA
                     IEASSEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLST
                     LKEIADNADNVLLDTIIENGFFIQKVEIINN"
     gene            12044..12760
                     /gene="agrA"
                     /locus_tag="NW953_RS09950"
                     /old_locus_tag="NW953_09950"
     CDS             12044..12760
                     /codon_start=1
                     /gene="agrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /locus_tag="NW953_RS09950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09950"
                     /product="quorum-sensing response regulator AgrA"
                     /protein_id="WP_000688492.1"
                     /transl_table=11
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVLE
                     QAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKVAA
                     MDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIMFFE
                     SSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKERIVYF
                     KNKEHCYASVRNVKKI"
     gene            complement(13134..14093)
                     /locus_tag="NW953_RS09955"
                     /old_locus_tag="NW953_09955"
     CDS             complement(13134..14093)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /locus_tag="NW953_RS09955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09955"
                     /product="carbohydrate kinase"
                     /protein_id="WP_001253292.1"
                     /transl_table=11
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTVQ
                     KLGQQAHMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERDFS
                     FYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGTVVF
                     DPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTV
                     VIYTKGADGAAVYLKNGINHYHSGYKVKSVDTTGAGDAFIGAVISRILATDVLNLTQLF
                     ENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(14090..15574)
                     /locus_tag="NW953_RS09960"
                     /old_locus_tag="NW953_09960"
     CDS             complement(14090..15574)
                     /EC_number="3.2.1.26"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /locus_tag="NW953_RS09960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09960"
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="WP_029550166.1"
                     /transl_table=11
                     /translation="MTEWTREERYQRIEDVDTDYFKTLKQQVDQSKFRQQFHIQPETGL
                     LNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYDSH
                     GVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGY
                     TSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDFGYMW
                     ECPDYFNVDNQDVILICPQGIEPKGDQFNNIYQSGYILGKFDIEKLTYEHENFVELDNG
                     FDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYP
                     ALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLI
                     AYNKRENKITLDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIFCNDGERVL
                     TSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     misc_feature    15685..15699
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    complement(15702..15715)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.36"
                     /tool="antismash"
     gene            complement(15723..16673)
                     /locus_tag="NW953_RS09965"
                     /old_locus_tag="NW953_09965"
     CDS             complement(15723..16673)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /locus_tag="NW953_RS09965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09965"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_258409286.1"
                     /transl_table=11
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDYQ
                     PNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEI
                     QALETLARSKVDGIVLMATDITERHIEIINKMNVPIVIVGQQHEQLHSIVHDDYKAGQI
                     IGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVEAQKD
                     VANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKT
                     IHYNYFEAGQCAIEEIQQMLKKQDMPYGVTVDVNI"
     gene            complement(16857..18107)
                     /locus_tag="NW953_RS09970"
                     /old_locus_tag="NW953_09970"
     CDS             complement(16857..18107)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008519 - ammonium channel activity
                     [Evidence IEA]"
                     /GO_process="GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /locus_tag="NW953_RS09970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09970"
                     /product="ammonium transporter"
                     /protein_id="WP_001052261.1"
                     /transl_table=11
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMA
                     AIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPLALFMLFQMM
                     FCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGT
                     VVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDQI
                     AMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSAT
                     IMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAFFQSKKANPDIENG
                     FIYTGDVHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGE
                     HAYFEGELNRFNKHIRY"
     gene            complement(18316..18540)
                     /locus_tag="NW953_RS09975"
                     /old_locus_tag="NW953_09975"
     CDS             complement(18316..18540)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /locus_tag="NW953_RS09975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09975"
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="WP_000581077.1"
                     /transl_table=11
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEAI
                     PNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            complement(18600..19679)
                     /locus_tag="NW953_RS09980"
                     /old_locus_tag="NW953_09980"
     CDS             complement(18600..19679)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /locus_tag="NW953_RS09980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09980"
                     /product="YeeE/YedE family protein"
                     /protein_id="WP_258409287.1"
                     /transl_table=11
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYALL
                     IAITIQSIGLLILTATGILQIPAHSFPILGTVIGSFIFGIGIVLAGGCATGTWYRAGEG
                     LIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVKSDMSQTTGIPFWGLVVILTIIT
                     IFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWPMSAST
                     GRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWRLPDKI
                     TIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMSHFIFVR
                     PMKKVQQQSAKVQQQTQIV"
     gene            complement(19977..20612)
                     /locus_tag="NW953_RS09985"
                     /old_locus_tag="NW953_09985"
     CDS             complement(19977..20612)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /locus_tag="NW953_RS09985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_09985"
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="WP_001283612.1"
                     /transl_table=11
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQID
                     SATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTYNF
                     SIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQKVA
                     DELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
ORIGIN
        1 ttgtgtttat cttttatacc aaaatttggt ataggcaaag gattattttt aagcttattc
       61 acatcagtat cagcttttaa taatgctgga tttgcccttt ttaagaataa cttaatagat
      121 tattctaatg atccaattgt cattatcaca atctcaatac tcataatatt tggaggtatt
      181 ggacattttg tcgtaataga ctttattaat tgtaaaaaat tgagtaaatt atctttacat
      241 tctaaattag tcttaactac aactagtatc ctaataatta taggagctat tacattcttt
      301 ttattagaac agtttaatac tatgcaaaat atgggactag ttgaaaaaat cggaaattct
      361 tttttccaat cagtaacaac acgaacagcg ggttttaaca gtattgatat agcaagcatt
      421 aacaaatcta ccgccttaat gttaatgcta ctaatgttta ttggtggtgc ccctctcagt
      481 gcagctggag gaattaaaat aactactttt gcagtagcgt ttatttttgt actaaattat
      541 atacgtaaag aaaataatgt tttcgtattc aataaagaaa tatctaacaa acatataaaa
      601 ctatctattg ttaccattaa tatctcattt ctatttatca gtatcattac ttttatatta
      661 tcgataatta atccgaacat atcattaatc aagttattat tcgaagtggt ttctgcattt
      721 ggaacagtcg ggttaagtat gaaccttacc acagaatatc atggtattac taaaataatt
      781 attatattcg ttatgctttg cggtaaagta ggactattaa ctttattaag aacatttata
      841 ccaccaaaaa gtccaaaaaa ttaccgctac actaaaggac aaatttatct ataaaataac
      901 tatccatctt taacaagaaa tgaactagtg ttttaatcta ttacatttaa aaaactacct
      961 aaacaggtag tttttttgtt attctgcatt tattttaggc acattaaatg tcttatctct
     1021 ttaattaagt aaaatgcaca agtacacctt taaaaagatt tactcaaaac tatactatac
     1081 aatgatttaa atatttaaaa tttatttttt actacgttaa cctcaattgt tgaaaagatg
     1141 aatggaaatg ataatttaat aatgtatact tttgattgtt taaacatgta cattttctaa
     1201 aattctagag ataattttat tgctgtgaac aacaatgaaa ttatcttcag acataaatac
     1261 gaattttgaa gttactactc tttcatattt acaaaaatta ataattgtta tgatgaactt
     1321 aaacatgaaa ataagcaaaa gtgaaatagc tagacacatg ggtgttgata gaagagcagt
     1381 tgataaatat tcaaatgtct ttgactttta ttttataaat tcttccataa aaataaccac
     1441 actcctaaat caataggtga gtgtggtttt gtgccttata ttttaagaat agccatcttc
     1501 aaattatttt cacttgtatg atctttgatt tctgtaaatc caaattttct ataaaattct
     1561 cttaatctat caacatcttc atactctatc caaatatatg aaccgccaaa tatgttcgca
     1621 ccttctaaaa ctttctcata tgctaaagtt aacaaatcgt ttccatttat ggattttgta
     1681 gccaatgctt cttcagaata attttttcct atttgagcaa ttaagtatcc ttgtatgatt
     1741 aaattgtcat ttccactgtg agtttcaccc ttttgatata acttcttctt taacgtgttt
     1801 gatattttat ctaacatacg ttttgtaaaa actaagggtt tattgctaat tgtaaaatag
     1861 ccaactagta tttgtttgct tttgaattgt gaaaaaacta aatgagttct agacaggttt
     1921 tgtctatcaa atgctataga cttatttttc aaaaataaat caacatcgtg cgctgattca
     1981 cttcctgaaa ttgatattgt ctcaaatgaa tttagtatat ttaaaactga aggtatcttt
     2041 cgcttaacct cttgcttatc acctgttaac aattctgaaa gtgatattac ttttacagtc
     2101 aattacttcg atagtccttc aatagatttt caatctcaca agttgatttt attttagtcg
     2161 cttttatgtt tgttttgttc acattactat tattttcgtt aatctccatt gcatctatta
     2221 gtttttgcgc tgattttttt gtgaatttat aatcagtagt aatacttatt gtagccatac
     2281 tactcacttc cttattttac ttcatttgac aaccaagtat acattgtcta cgttgtcttc
     2341 ttgtttacaa tgtatcatga aagcatcttt tcgacaatag attcaataaa tttctttagt
     2401 gtatatgatt ttgcacttaa accaatttga ttcttagcat aaaaacaccc atgctgtaac
     2461 tagtgtgggt gttttcaaaa tgcagacagc ttcttactac aatacgcaag tgagtcctct
     2521 gcaaagccga attgattacc ggaaatctaa ttacattaca acatactaga agaaactcct
     2581 tatatactca aaaggtatac taaacttctc tatataatct atatgatttt aacattaaaa
     2641 aaaccccatt atgaatctca ataacacatc agcacataat aaagacgcct tccaaaagtt
     2701 ttggtaacta atggaaaacg tcttaaaaac atattttcat acgctctaat atttatttgg
     2761 aacgaatcat actcgtgcat aatggaatgt tctagttagt caaaactata aacagtttta
     2821 catcattcct ggcatgccac ccatgttagg ttggtcatta tttttttcag gaattgaagc
     2881 cactactgct tcagtagtta agaacattgc tgcaacactt gctgcatgtt gtaatgctga
     2941 gcgtgttact ttagttggat caacgatacc tgcttctaac atattaaccc actcgtttgt
     3001 agcagcgtta aaaccaacac ctggttctgc atttttcaag cgttcaacga tgacagagcc
     3061 ttctaaccct gcattttcag caatttggcg aactggtgca gttaatgctt taagtacaat
     3121 attcacacct gtttcaatgt cgccttcagc ttcaatttca cttacttttt ggtaaacatt
     3181 tactagtgca gtaccaccac ctgcaacaat accttcttca actgctgcac gtgtagagtt
     3241 taaagcatct tcaatacgta atttacgttc tttaagttct gtttcacttg ctgcacctac
     3301 cttgataact gcaacaccgc ctgctaattt agctaagcgc tcttgtaatt tttcacgatc
     3361 aaagtcagat tcagtttctt caatttgaga tttcaattgg cttacgcgtg catcaatgct
     3421 gttttcgtca ccgtcaccat caacaacagt tgtattatct ttagttactt ctactttact
     3481 tgcagtacct aacatatcta ttgatgcatc ttttaaatct aagcctaaat catcagtaat
     3541 cacttgcgca ccagttaaaa tagctaaatc ttcaagcatc gcttttctac gatcaccaaa
     3601 accaggtgct tttacagcaa cagctgtaaa tgtaccacgc atgcggttta gtacgatatt
     3661 tgttaatgca tcgccttcaa cttcatcagc tacaattaag attggacgat tagattgaac
     3721 cacttgttct aataaaggta agatatcttg gaaagaagag atttttttat ctgttactaa
     3781 aatatatggg cgttctaatt cagcaaccat tttatctgaa tcagtaacca tatacggtga
     3841 ttgataacca cgatcaaatt gcataccttc aaccacttca agttcagtgt tcaagccatt
     3901 tgattcttca attgtaatga caccatcgtt acctactttt tccatagctt ctgaaatata
     3961 gcgtccaata tcttcatctg ctgctgaaat cgcacctact tgcgcaattt catttttatt
     4021 ttcaactttt tgagaatttt catgtaacgc ttcaacagca actttaactg ctttgtcgat
     4081 accttgtcgt aaaccaactg ggttcgcacc acttgtaaca tttttcaagc cttcttgaat
     4141 cattgcttga gctaatactg ttgcagttgt cgtaccgtca ccagcaattt catttgtctt
     4201 atttgcgact tcttgaacta gtttagcccc catattttca tatggatctt ctaattcgat
     4261 ttctttagca atcgttacac catcgttcgt aattaaaggt gctgtaaact ctttatctaa
     4321 tacaacatta cgtcctttag gaccaatcgt tactttaact gcatttgcta attggtcaac
     4381 accacgtaac attgcttgac gtgcatcttc agagaatttc aattgtttaa ccataaatga
     4441 taaacctcca tttgtttagt catatttcat tttcgttctt tacaatctat ctatgaatta
     4501 tattctatat tattcaatta ctgctaaaat atcttcttca tttaaaacta aataagtctc
     4561 attatctcgt ttaacttctg tacctgcata ttgttggaac acgacacggt ccccttcttt
     4621 cacttcagga gtcactcttg taccatcatt taataggcgt cctgttccta ctgcaacgat
     4681 aacgccttcg tttgattttt ctttagcact atcagttaaa acaataccac ttttagttgt
     4741 ttgttcttgt tcttttttct caataatcac acgatttcca attggtttta gcatgattgt
     4801 tcctccttaa aaaacctaaa gtttagcact taactttata gagtgctaac atacatttat
     4861 aataatcaaa tttggtcaaa acttcaagtc ataacctttc aattaagttt tgtaacttca
     4921 agttatttac gataaaataa attataaaca aatatttgga ggaaaattat gacaagacta
     4981 tgggcatcat tgctaactgt tattatttat atattgtctc aatttttacc gcttctcatt
     5041 gtaaaaaaat taccatttgt acaatatagt ggcatagaac tgactaaagc agtcatttac
     5101 atacaacttg ttctattttt aatcgccgcc acgacgatta ttttaattaa tttaaaaatt
     5161 aaaaatccaa caaaattaga attagaagtt aaagaaccta aaaaatatat cattccatgg
     5221 gcattgcttg gatttgcact ggtaatgatt tatcaaatgg tagttagtat tgtatatact
     5281 caaatttttg gcacacaaca aacaagtcca aatactgagc gtttaatcgt tattgcacgt
     5341 aagattccat tgtttatctt tttcgtatca atcgttggtc ctctattaga agaatatgta
     5401 tttagaaaag taatctttgg cgaattattt aatgcaatta aaggtaaccg tatcgtcgca
     5461 tttgctattg caacgactgt aagctcatta atctttgcat tagcgcataa tgactataaa
     5521 tttattccta tttactttgg tatgggcgtc attttttcat tagcatatgt atggacgaaa
     5581 cgacttgctg ttccaattat tattcatatg ttacaaaatg gatttgtcgt tatattccaa
     5641 ttacttaatc ctgaagcttt gaaaaaagcc acggaacaag cgaattttat atatcacatt
     5701 tttattccat aatataaaaa acgaatcaca cacggattat cgtcgctgtg attcgttttt
     5761 atttttcttt ttatttcgct ttacaaataa gaatagcatg attgaaaata ccaccatcat
     5821 agaacataca attgctagcc aacttttact atatgcagtt gacgcacttt tcttattatc
     5881 ttcttgtttt tttacaactg taaaatcatc tttttcatga ggctcaaatg aacgttgttt
     5941 tgaatccggc ttatcttcgg gctttttcaa atctggcatt gatccaattt caccagtaat
     6001 taatgcgagt acttgttcat ctaaattacg atagtacctt tctgatttca atggattgaa
     6061 atattggaat ctaaagtaat tcgattgctg ttgtaatcca tttaaatatg catttccgct
     6121 gaagttttgt ttgcgaataa tattataaat gtcttcatca gtaacttctc cataatcttt
     6181 gcccaactta ttaatttgat ttaaaatata cggattatac ttgtaagccc cattcgccaa
     6241 ggctaaaaat cgttgggata caaaatcatt accagtagga ttttgtgagt ttccttgatt
     6301 tccatttggt tgccattgac cttgattaga tccatctgaa gcatttgggt tccatgttcc
     6361 cccatttttc gagccaccag aatgattgga atccccaggt tgattgggat ttggtgacgg
     6421 gtttggattt ggcttattag ggtctggttt tggattcgga tttggatctg gttttggatc
     6481 cggatttggt ttcggcttat ctgggtctgg ctttggttcc ggatttggct ttggtttatc
     6541 tgggtctggc ttcggttccg gatttggttt cggcttatct gggtctggct tcggttccgg
     6601 atttggtttc ggcttatctg ggtctggctt cggttccgga tttggtttcg gcttatctag
     6661 gtctggcttc ggattcggac ctgggtttgg gttatttggg tcaggttttg gttctggacc
     6721 tgggtttggg ttatttgggt caggttttgg ttctggactt gggttttggt tatttgagtc
     6781 tggcttcaca ttattcgaat caggattagc atcacgacta ttcgaatttg ttccttcatt
     6841 acccgtttga tttgattcgt gaggtgtccc agtctcatta ttatttattt cactatttac
     6901 tgatggtgaa gttacattat gattcaaatt agtagttggc ttttctccat ttaagttatc
     6961 ctttgcttca actgtgtgcg ttgcaagccc taaactacta ctacttaaaa ttaatatact
     7021 tgttgaaagt attgttttct ttaaccaatg atatgacatt tcacgagaca cctccctttt
     7081 tcatataagt attaatctaa gtataataaa tatgcgttga aaatgcattt atatttggaa
     7141 tttgaaaatg aatattaata ttcttgaaat tttcgtaaat attactttac aagttcgagt
     7201 ataaatttta tactaagtct attaagtatt ctttactaaa gaaggagtaa tgattatggg
     7261 tttatttaaa aaagataaaa aagcaatgac ttttgacaac gcaatggaag aaagacgttc
     7321 aatatataat ttgaaagatt caatatcaat tagcgatgac gaattagaat caatgattgc
     7381 acatgcagtt aaacatgttc cttcatcatt taattctcaa tcaactagaa ttgttctctt
     7441 attaaatgac aacaataata aattttggga taacacaaaa gcaattttaa aagaagtcat
     7501 gggagagaat cgtgatttcg agccaactga acaaaaaatt gataacttca aacattcata
     7561 cggtacaatt ttgttttatg aagatcaaga tgttgtaagt ggtttacaag aacaaatgcc
     7621 aaactactat gataattttg caatatggtc aactcaaaca aatgccatgc atcaatttgc
     7681 aatttggact gcattagcta ctaaaggcat aggtgcttca ttacaacact ataatccact
     7741 tgttgatgag atgacttcaa atgaatttaa tattcctaaa agttggaaac ttattgctca
     7801 aatgccattt ggtgatattc gtgaagctgc tggcgaaaaa acatttaatc cagtggaaga
     7861 tcgtttcgta ataaaaaaat aatcaatata ttgttgggaa tgggacagaa atgatataga
     7921 atcattagtt attcattatg tagtagttct tacacattag ccgctgctaa tgtgtactta
     7981 agaataggaa tacatgagta aaactcatgt ataagaaata ctaatttcta aagaaaaagt
     8041 atttctttac gttagagccc accccaactt gcattgtctg tagaaattgg tgttccaatt
     8101 tctctatgtt ggggccccat caactactgt caatataaca ttgtagagcc taggactttg
     8161 attttatgtc ctgggcttgt tttttgcatt tcaacataac ttttagtatg ttatatataa
     8221 gatctatcaa ggatgtgatg ttatgaaagt ccaaatttat caattaccga ttatttttgg
     8281 agatagttct aaaaatgaaa ctcaaataac gcaatggttc gaaaaaaata tgaatgctga
     8341 agtagatgta gtcgttttac ctgaaatgtg gaataatggc tatgatttag agcatttaaa
     8401 tgaaaaagca gataataatc taggacaaag tttttcattt attaaacatt tagctgaaaa
     8461 atataaagtc gatatcgtag ctgggtcagt ttctaacatt agaaacaatc aaatatttaa
     8521 tacagctttt agtgtgaata aaagtggcaa actgattaat gaatacgaca aagttcattt
     8581 agttcctatg ttaagagagc atgaattttt aaccgctggc gaaaacgtag ctgaaccctt
     8641 tcaattgtct gacggtactt atgtgaccca acttatctgt tatgatttac gttttccaga
     8701 attattaaga tatcctgctc gtagtggtgc taaaattgca ttttatgtgg cacaatggcc
     8761 tatgtcacgt cttcaacatt ggcattcttt actaaaggca cgtgcaattg aaaataatat
     8821 gtttgtcatt ggaactaata gcactggatt cgatggtaac acagaatatg ctggtcattc
     8881 gatagttata aatccaaacg gagatttagt tggagaatta aatgaatccg cagatatttt
     8941 gactgtagat ttaaacttaa atgaagtaga acagcaacgc gaaaatatac ctgtatttaa
     9001 aagtattaaa ttagatttgt ataaataaaa agcacacgaa ataatattta acacatgtca
     9061 tgactaaaca tagatttatg agacgatttt tcataagcct atgtttattt ttttataata
     9121 gataactcag taagaaccag tttcgccctt agcaaatgaa tgattttctt aattaaattg
     9181 attatttttt tgaccttttc caacattaga cttattcata tattttaacg gtggatctca
     9241 taatgaggat taactcttcc ctactttatt acaataaaaa aaggccgcga gcttgggagg
     9301 ggctcacgac catacttatt attaagggaa tgttttacag ttattttttc aatctatttt
     9361 tggggatgtt attaattatg aaaaaaaatt ttatttatca aataatgata aatcgatgtt
     9421 gtttacgata gcttacatgc tagaaataat tatctgtaat gataattaag aaaaatacat
     9481 agcactgagt ccaaggaaac taactctact agcaaatgtt actcacttgc taattttaca
     9541 tggttattaa gttgggatgg cttaataact catactttta gtactatacg aagataacaa
     9601 atttacaatg aaagtaatta attattcatc ttatttttta gtgaatttgt tcactgtgtc
     9661 gataatccat tttactaagt caccgattgt tgaaatgata tcttgtgcca ttgaaatcac
     9721 tccttcctta attaagataa aaattcttaa aattaaacaa ctcatcaact attttccatc
     9781 acatctctgt gatctagtta tattaaaaca tgctataagc atttattttc cattttttct
     9841 taactagtcg ttttttattc ttaactgtaa atttttttat gttaaaatat taaatacaaa
     9901 ttacatttaa cagttaagta tttatttcct acagttaggc aatataatga taaaagattg
     9961 tactaaatcg tataataaca gtgaggagag tggtgtaaaa ttgaattatt ttgataacaa
    10021 aattgaccag tttgccacgt atcttcaaaa gagaaataac ttagatcata ttcaattttt
    10081 gcaagtacga ttagggatgc aggtcttagc taaaaatata ggaaaactga ttgttatgta
    10141 tactcttgcc tatattttaa acatttttat cttcacttta attactaata tatcgtttta
    10201 tctaattaga aggtatgcac atggtgcaca tgcaccttca tctttttggt gttatataga
    10261 aagcattaca ctttttattg ttctacctct attagtacta cattttcata ttaatgaaac
    10321 actaatgatg tttttagcat tattaagtgt tggcgtagtg attaaatatg cacctgcagc
    10381 tactaagaag aagcctatcc cggcaaggct tgtaaagcaa aaaagatatt tttcaattat
    10441 aattagtaca attcttttca ttatcacact ttttgtaaag gaaccatata cccaatttat
    10501 tcaactgggt attattatac aagctattac attactacca atatactatt caaaggagga
    10561 ctaaattatg aaaaaattac tcaacaaagt tattgagctt ttagttgact ttttcaacag
    10621 catcggttac agagccgcct atataaattg tgatttttta ttggatgaag ctgaagtacc
    10681 aaaagaatta actcaattac acgaataact ataagagaaa gtgtgatagt aagtggaagc
    10741 attaaatgat tataattacg ttttatttgt aattgttcaa gtttcattaa tgttctttat
    10801 atctgcattt attagtggaa tacgatacaa aaaatcagat tatatataca ttattggaat
    10861 agtgttatct tcagtatatt tctttgacaa aatcggaagt atttcactag ttgttataac
    10921 gatttttata ataatttttc tctattttaa aattagacta tattctgtat ttttagtaat
    10981 ggttactcaa atcattttat attgtgcaaa tttcgtttat atcattattt tttcatacat
    11041 tataacaatt tcacacagcg tgtttatagt tctaccaatc tttttggtag tgtatgtatc
    11101 aatttcttat gcacttgcct acattttaaa tagaatatta aaaaggataa atggtacata
    11161 cctttctctt aataaaaaat tccttactgt tataactatt gttatagtaa taacattctc
    11221 tttgttattt gcatattcgc aaattgatgc gagtgatgca tcaacaatca aacagtattc
    11281 gcttttattc ttgggaatta ttatattact gagtatctta atttttattt attctcaatt
    11341 tactcttaaa gaaatgaaat ataaacgtaa tcaagaagaa attgaaacct attatgaata
    11401 cacattaaag attgaagcaa ttaataatga aatgcgcaag ttccgtcatg attatgtcaa
    11461 tatcttaacg actctttcag aatacattcg tgaagatgac atgaccggtc ttcgagacta
    11521 tttcaataaa aatattgtac ctatgaaaga taatttacaa atgaatgctc ttaagttaaa
    11581 tggtattgag aatcttaaag tacgtgagat taaaggtctt ctaacagcta aaattttacg
    11641 tgcacaagaa atgaatatac cgattagtat cgaaatacca gatgaagtaa ctcgcattaa
    11701 tttgaatatg atcgatttaa gtcgcagtat tggtattatt cttgataacg caatagaggc
    11761 atcaagtgaa attgatgatc ctatcattcg agttgcattt attgaaagcg aaaattcagt
    11821 aacgtttatt gttatgaata aatgtgcaga tgatatacca cgtattcatg aattgttcca
    11881 agaaagtttt tctactaaag gtgaaggtcg tggtttaggt ctatcaactt taaaagaaat
    11941 tgctgataat gcagacaatg tcttgttaga tacaattatc gaaaatggtt tcttcattca
    12001 aaaagttgaa attattaaca actagccata aggatgtgaa tgtatgaaaa ttttcatttg
    12061 cgaagacgat ccaaaacaaa gagaaaacat ggttaccatt atcaaaaatt atataatgat
    12121 agaagaaaag cctatggaaa ttgccctcgc aactgataat ccttatgagg tgcttgagca
    12181 agctaaaaat atgaatgaca taggctgtta ctttttagat attcaacttt caactgatat
    12241 taatggtatc aaattaggca gtgaaattcg taagcatgac ccagttggta acattatttt
    12301 cgttacgagt cacagtgaac ttacgtattt aacatttgtc tacaaagttg cagcgatgga
    12361 ttttattttt aaagatgatc cagcagaatt aagaactcga attatagatt gtttagaaac
    12421 tgcacataca cgcttacaat tattatcaaa agataatagc gttgaaacga ttgagttaaa
    12481 acgtggcagt aattcagtgt atgttcaata tgatgatatt atgttttttg aatcatcaac
    12541 aaaatctcac agactcattg cccatttaga taaccgtcaa attgaatttt atggtaattt
    12601 aaaagaactg agtcaattag atgatcgttt cttcagatgt cataatagct ttgtcgtcaa
    12661 tcgtcataat atcgaatcta ttgattcgaa agagcgtatt gtttatttta aaaataaaga
    12721 acactgctat gcatcggtga gaaacgttaa aaaaatataa taagataata aagtcagtta
    12781 acggcgtatt caattgtaaa tcttgttgga ttttaacaag ataactagca aatgcactgt
    12841 atagctggct ttttattttt taatagcaaa aatttaattt aaagcgtaaa tttaaataaa
    12901 acgatacaac gtttcattgt atacctattc ctattactac ataatttatc aatttctatt
    12961 tcactctaca atcctatagc gcaattttta acaaaatata ctaaatttga aatcgacagt
    13021 ttccataaat aaaatgctac aatatcctac ccaataaatc ttattttgct cgcaaaaaaa
    13081 cagtgtagca caagattcaa cttgtttcct acactgtttt ataaatgaat tatttaaaat
    13141 gcttgttcaa cctcttgtaa tgttggcaaa ctattaatag caccatactt cgttgtcact
    13201 attgcagcaa cgcgattgct aaacgctaaa atttcctcac cttcattttc aaataactga
    13261 gttaaattta acacatcggt tgctaaaatc ctgctaataa ctgcaccaat aaaggcatcc
    13321 cctgccccag ttgtatcaac agattttacc ttatagccac tatgataatg attaatacca
    13381 tttttcaaat atacggcagc accatctgct cctttcgtat aaatgactac agtgacatta
    13441 cctgtaaata atgattgaat ggcttcattt tcatcatgaa tacctgtaat aaattctaat
    13501 tcttcatctg aaacttttac aatatgcgcc aatggcaaaa aggtatgaat agtttgtctt
    13561 aaatcttctg cgttatccca taatggcaat cttacattag gatcaaatac gacagtacca
    13621 tttgcattta atgttttggt aatcaattga taatgcgcat ctctcattgg actatcgacc
    13681 aaatcaacag aacaaaaatg tactacatcg ttttcattca catcaatatc attcacaaaa
    13741 cttggttcaa ataacatatc agcagagggc ttacgataaa aagaaaaatc tcgctctccc
    13801 gcttcagtca aactcacaaa agctaacgca gtgtttgctt cattcgtgcg atacactttt
    13861 gatacatcta caccaatact tgatatcgtt tcaataatgc tatctccaaa tgcatcatta
    13921 cctaattgtg taatcatatg cgcttgttgt cctaactttt gaactgtaca agctacattg
    13981 catggcgcgc caccaatttg cttcgtaaat gtttgaacat cttttaaatt cgcatttgta
    14041 atatttggaa taaaatcaat taacgcttct cctattgaaa ataaacgtct catttatgtt
    14101 catcctttaa atcatattta gtaaattgta aatatacttg ccctgattct gttgatgttt
    14161 taatacctag tgcgtcttct gtcgggaaaa ttcgagatgt caaaacacgt tccccatcat
    14221 tacagaaaat ttctatacta gatgtatcaa caaaaatttg taattgcttt aatggcgttt
    14281 ctaatatcgt actacgcgtc gtaccttcaa catttgtagg caataaacca ctgtcgctgc
    14341 gatctaatgt tattttattt tcacgcttgt tataagcgat taatgttgaa gaagtcttag
    14401 acgtacgtaa ttcaaagtat acttcggtag catcattatc caaaatatct atgattaatt
    14461 catactgttt accttcatac ggatgtaatt ttcgagtaaa tttatttgcg tagcctaatg
    14521 ctgtctcttt attgtgacgt aatttttcca acgctggata tggacgttgc ttaagttgac
    14581 cattttctac atttaataca cgaggaattg ttaagcaatg ggcccatcct tcattatcag
    14641 taggatattc gatttccggt aaccccatcc atccaattag tactcgtcgg cctttttcat
    14701 ctaaaaatgt ttgaggtgca tagaaatcaa aaccattatc aagctcgaca aaattttcat
    14761 gttcatatgt taacttttca atatcaaact ttccaagtat ataaccactt tgataaatat
    14821 tgttgaactg atcgcctttt ggttcaatac cttgtggaca aataagtatg acatcttgat
    14881 tatctacatt aaagtaatct gggcattccc acatatatcc aaaatcatct aactctgtat
    14941 ttatttcacc taaataatgc caattaatta tatcttcagt attataaagt aataatcgac
    15001 cttgctgatc attattttgt gcgccaatga tcgcataata tttctcacca tatttaaaaa
    15061 ctttaggatc tctaaaatga ctcgtatatc cttctggttg ctgactaatt actggctttg
    15121 gaaacttttc aactgaaccg tcttctttca atcgtgcgat catctgactc gcatggcgtt
    15181 gccaatgatt atctcgatga tttcctgtgt acatataata taaatgaccg ttatattcaa
    15241 aagcgctacc gctatataca ccatggctgt catatttggt atctggattt aaaattggcc
    15301 cttcagcttt aaagtttatt aagtcatcac tcgtgtagtt ataccaatac tttaagccat
    15361 gtactgcgcc taatgggaac cattgatgtg aaacataata ctttccttta taaaaaataa
    15421 gtccgttggg atcgtttaat aagcccgttt ctggttgtat atgaaattgt tgacgaaatt
    15481 ttgattgatc aacttgttgt tttaatgttt taaaataatc agtatcaacg tcctcgattc
    15541 gttgataacg ttcttctcta gtccattcgg tcataatatt cacccagtcc tccttttata
    15601 atttatgctg ttaacaattt atattctatt atagcaaatt ttacctacgt tctttaactt
    15661 ttaacctatc catttatact tttatggtat cggttccaca tttattttaa aaaatacagc
    15721 gtctaaatat taacatctac tgtgacgcca tatggcatat cttgcttttt aagcatctgt
    15781 tgtatctctt ctatcgcgca ttgaccagct tcaaaataat tataatgaat tgtttttatc
    15841 gatggagaca ctaattgtgt cattggatca ccaccaaaac catatatttg atgtggtttc
    15901 ataacatctt ttttatcaga ataatattta taggcagcta atgcaatcgt atcagttgct
    15961 ccaacaaccg catctacttg ctctacattt tccaaaacat ttgcaacatc tttttgtgct
    16021 tccacataag taaaatttgt ttcatgtata ttaggtttaa tttgatattt ggctaactgg
    16081 tcaagtaaac cacgttttct atgtatacca actgcaatat ctttttcact cacactaaac
    16141 acttcaactt gttgatatcc ctgttgacca atccattcac ctataatttg acctgcttta
    16201 taatcatcat gcacaatact atgcagttgt tcatgttgtt gaccaacaat aacgattggt
    16261 acattcattt tattaatgat ttcaatatgt ctctctgtta tgtctgtagc cattaaaaca
    16321 ataccatcta ctttactgcg tgctaatgtt tcaagcgctt gtatttctgc ttcgatattt
    16381 aaacctgtat aatttaaaat taattgtgat tcatattttt ggcattgttt tgccaatcct
    16441 ttgattgttt catctactgc atatgaattc attctaggta taatggcacc aataaggtgt
    16501 gtttgtctcg ctcttaagct ttgagcaaat tgattcggtt gatagtcatg ttctgctata
    16561 atttttgtta atttttcact tgttttttta ctgacagatc cattatttaa aaatctagat
    16621 actgtacttt ttgaaacgcc tgccaatttg gcaatatcag atatattttt catacttatt
    16681 cacctatcat tatttgtgac acttagcctt attttagcat atgtgtaacc gctttaaata
    16741 atatttctgc ttctttcata aaaagatttc aaaataaaat ttctgtataa caaaatgtcc
    16801 caacatcaac ctttagttaa atgtcaggac aatgaatatt ctatgtatat ataaatctaa
    16861 tatcgaatat gtttattgaa tctatttaac tcaccttcaa agtaagcatg ttcaccatga
    16921 acaatcttgt ctaaccctat attcgtttct tgttctgtaa cagataatgg tgtaattaat
    16981 ttaattactt tcgcaataat aaacgtcatg acgatgctaa aaattacaac tgctgttaca
    17041 cataatattt gcacaagtat aatatgcacg tcaccagtat aaataaaacc attctcaata
    17101 tctggatttg cttttttact ttggaaaaag gctgttaata ctgcaccaat aataccgcca
    17161 acaccgtgga taccaaatgc atctaatgca tcatgatact ttaattttac ctttatgtaa
    17221 ttgatgacaa tataacaaca gataccccct attaaagcca tgattgttgc actaagatat
    17281 gttacatatc cagctgcagg tgtaataaca actaatcctg ctaatgcacc gagtaaaagt
    17341 ccaagtaaac ttgtcgtctt tttaaaaata tattctaaaa ttaaccaacc aatagccccg
    17401 gcactggcag aaataactgt attcgtaaat gcaagcattg caatttgatc aaatgtaaaa
    17461 gcactgccta cattaaatcc ataccaacca atccacacga atataccgcc aatcaacgta
    17521 atgatgagat tatgtggtgt tgattcagaa tgtttgtttc cttttccaat cattatagct
    17581 aatactaaac cagaaacacc tgatgtaata tgaacgactg tacctccagc gaaatctaat
    17641 acaccgagtt tgttaatcca accgccgccc caaacccaat gtgctactgg actgtataca
    17701 agagcagtcc atattactac aaataataaa taaggaataa acttcatttt ctcagcgatt
    17761 gaacctgata atattgagat tgcaatcgta caaaacatca tttggaataa cataaataat
    17821 gccaatggaa tatgcggact aatatcttct tgtgtcgcga aacctacatg attaagaaat
    17881 gtatattccc agttaccaaa ccataaattc ccttccccaa aactaattgt aaaacctact
    17941 gttatccata caaatgtaac aagcacaatt gctgccatac tttgcatgac agtatttagc
    18001 gcatttttag attgaactaa cccaccataa aataaactta atcctggtgt cattagccaa
    18061 actaataatg tacacaaaaa cataaatatc gtatcgttaa gattcatact taccactccc
    18121 ttgttttcat aataatctga taatttataa aaagcaaaaa tgcgtaaagt tttattacat
    18181 cttttggaat aaaatgtgcc acgctatata aaaaatgatt tcatgaacta aaacaaaaaa
    18241 gactacttac tcgcttttaa agttgcggaa gtagccatag aatctaatat ctattttatt
    18301 attaaaatga taacgttaaa ctttttgaat tgtaattgtc catgaagcat tatcaatttg
    18361 ttcatagttt gttacaggat atccattttc tgcagcccaa tttggaatag cttccgtcgc
    18421 ttgtgtgcaa tcaaaatcaa tttttaattc atctccagat tgcaatgttg ccattttctt
    18481 ttgcgcctca attaacggaa atggacatac cattcctact gtacctaatt cgtgtatcat
    18541 aattatttct ccttttccaa tcacttttat ttgatcaaca tcatttgcat aataatcttc
    18601 tatactattt gcgtttgctg ttggaccttt gcagattgtt gttgtacttt tttcattgga
    18661 cgaacaaaga taaaatgact cattgtccat accccaacta tcatcgatgc tagcgcaatc
    18721 catccttgcc aagtcatcgt tgccgtttca accaaaccgt taccgataga acaaccacca
    18781 gcaactgacg caccaaatcc catacatatg ccaccaattg cactgtttcg tattgtaatc
    18841 ttgtctggca atcgccattt aaattctctt gatcctctag ctgcaatata tgaaccaatg
    18901 aaaattccta gaactaaaaa gacaccccaa tcaataaatt tagtttcacc tgtaatcaaa
    18961 aagtgcacta aatttgctga tggcgttgtt atacctaaac cgtcatttct tccagttgat
    19021 gcactcattg gccaagctaa gagtgcgata agtccaattg caattgctgc aataaatgga
    19081 tggtatcgtt tttcgaaaag gtaatatcta atacctgtat aacgttgctt tagttttgga
    19141 acagcaactc gaactttctt attattgagc gttcttacaa cgagaaaaat ggtgattata
    19201 gttaatataa cgactaatcc ccaaaacgga atgcccgttg tttgagacat atcacttttt
    19261 acattcgttg gttgattaat tttatccatt actggcttta aaatccctgt tttagtgatt
    19321 gctgcagtaa cagcatataa tactaatgca atccaactac caattagccc ttcaccagcg
    19381 cgataccaag tacctgttgc acaccctcca gccaatacta ttccaattcc aaaaataaaa
    19441 gaacctataa ctgttcccaa tattggaaaa ctatgtgcag gaatttgtaa aatacctgtt
    19501 gccgtcaaaa tcagtaatcc tatactttga atagtaatag cgattaataa tgcatagaac
    19561 atcttattat tcttttgcac atacatatct cgaaagccac ctgttaaaca aaatcttgtt
    19621 ctttgcatca caaaacctaa gagtcccccg acaattaagc cactaaaaat catccacata
    19681 atcctccccc taatttccga taagtttagt atgtattata ggcgatgatt ttcttgtttt
    19741 caaccttata cacaatattt ttgatattta tattatgttc atataagaaa tgcgattaat
    19801 tacgattcat cattatatct ctacgtgtta atttattacc tatattatat ttatcaacac
    19861 aacagttttc cctttcattt taaaacataa cagaaaaagc ctgagacata cttagtccca
    19921 gactgttcta taattaaaga tataacaagg ttaatcattc aaagctaaca tcacatttat
    19981 tcactgtaat ttttcataaa gaataataat gactgtaatt ctatacctaa gtcaatttga
    20041 tgtacttgca catctgaagg cgtatttatt ctaccaggag tgaagtttaa aatacctttc
    20101 acaccagctt ggacgagttc atctgcaact ttctgtgcaa ctctttctgg tgtagttaga
    20161 atcacaacat ctatttcttc cttcttcaat gttgttatta attcatcgtt atcttttaca
    20221 ataacgttcc ctattttctg gccaataaca tcttctttta cgtcaaacgc ttctgtaatc
    20281 gtcatatcgt catgtattga aaagttatat gtgagcaaag ctttccctag gttcccaact
    20341 ccgacaattg cgattttgat catgtcactc tcgcttagtt cagatttaaa gaaatccaat
    20401 aaactatcta tattatatcc gtaccctttt ttacctaatt cgccaaaata tgaaaagtca
    20461 cgacgaattg ttgccgagtc aatttgtaac gcatcgctaa tcgcttttga atttacacga
    20521 tctatacctt tagattttaa tgaactgaca aatctataat ataacggcaa acgttttaaa
    20581 gttgctcgag gaattttaac ttggtcactc attcgctatt tcctccttcg tgtttgaatg
    20641 aattatatct atgtattcaa acgagttaga gcgtaattgt tagattatta aaaaacaaat
    20701 aattgatt
//
