LOCUS       NZ_CP102975            10285 bp    DNA     circular CON 10-JUL-2024
DEFINITION  Staphylococcus aureus strain 16CS0209 chromosome, complete genome.
ACCESSION   NZ_CP102975
VERSION     NZ_CP102975.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2769676)
  AUTHORS   Monecke,S., Roberts,M.C., Braun,S.D., Diezel,C., Muller,E.,
            Reinicke,M., Linde,J., Joshi,P.R., Paudel,S., Acharya,M.,
            Chalise,M.K., Fessler,A.T., Hotzel,H., Khanal,L., Koju,N.P.,
            Schwarz,S., Kyes,R.C. and Ehricht,R.
  TITLE     Sequence Analysis of Novel Staphylococcus aureus Lineages from Wild
            and Captive Macaques
  JOURNAL   Int J Mol Sci 23 (19), 11225 (2022)
   PUBMED   36232529
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769676)
  AUTHORS   Braun,S.D., Diezel,C., Reinicke,M., Linde,J. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2022) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 181x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024741735.1-RS_2024_07_10
            Annotation Date                   :: 07/10/2024 04:01:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,704
            CDSs (total)                      :: 2,622
            Genes (coding)                    :: 2,539
            CDSs (with protein)               :: 2,539
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 40 of 83
            Pseudo Genes (incomplete)         :: 40 of 83
            Pseudo Genes (internal stop)      :: 17 of 83
            Pseudo Genes (multiple problems)  :: 14 of 83
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:15:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 963615
            Orig. end                         :: 973900
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102975.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10285
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10285
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10285
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5285](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5285
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(209..222)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(596..611)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.81"
                     /tool="antismash"
     tRNA            complement(623..711)
                     /anticodon=(pos:complement(964289..964291),aa:Ser,seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="NW953_RS04560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="NW953_04560"
                     /product="tRNA-Ser"
     gene            complement(623..711)
                     /locus_tag="NW953_RS04560"
                     /old_locus_tag="NW953_04560"
     misc_feature    complement(649..658)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 15.28"
                     /tool="antismash"
     tRNA            complement(714..788)
                     /anticodon=(pos:complement(964369..964371),aa:Asn,seq:gtt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="NW953_RS04565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="NW953_04565"
                     /product="tRNA-Asn"
     gene            complement(714..788)
                     /locus_tag="NW953_RS04565"
                     /old_locus_tag="NW953_04565"
     gene            complement(930..1499)
                     /locus_tag="NW953_RS04570"
                     /old_locus_tag="NW953_04570"
     CDS             complement(930..1499)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /locus_tag="NW953_RS04570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04570"
                     /product="competence protein ComK"
                     /protein_id="WP_000287265.1"
                     /transl_table=11
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFKV
                     QKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIWLN
                     MHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMISKNP
                     DQPIDYNKATLNVFEALTRYSLFEDK"
     misc_feature    1566..1579
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.83"
                     /tool="antismash"
     gene            1709..1927
                     /locus_tag="NW953_RS04575"
                     /old_locus_tag="NW953_04575"
     CDS             1709..1927
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /locus_tag="NW953_RS04575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04575"
                     /product="IDEAL domain-containing protein"
                     /protein_id="WP_000876826.1"
                     /transl_table=11
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELID
                     DALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            complement(2008..2994)
                     /locus_tag="NW953_RS04580"
                     /old_locus_tag="NW953_04580"
     CDS             complement(2008..2994)
                     /EC_number="6.3.1.20"
                     /GO_function="GO:0017118 - lipoyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /locus_tag="NW953_RS04580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04580"
                     /product="lipoate--protein ligase"
                     /protein_id="WP_124780244.1"
                     /transl_table=11
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVG
                     KNQNTIEEVNQSYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTE
                     PIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNAL
                     KVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDW
                     ENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFG
                     VGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            3193..3369
                     /locus_tag="NW953_RS04585"
                     /old_locus_tag="NW953_04585"
     CDS             3193..3369
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /locus_tag="NW953_RS04585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04585"
                     /product="YkvS family protein"
                     /protein_id="WP_000214898.1"
                     /transl_table=11
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLPE
                     KTVINHKRYKIVE"
     gene            3384..3986
                     /locus_tag="NW953_RS04590"
                     /old_locus_tag="NW953_04590"
     CDS             3384..3986
                     /EC_number="3.4.26.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /locus_tag="NW953_RS04590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04590"
                     /product="CPBP family glutamic-type intramembrane protease"
                     /protein_id="WP_001033862.1"
                     /sec_met_domain="Abi (E-value: 9.2e-08, bitscore: 29.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAGI
                     SYVFYQRDIESKRLLTSIGVGIITAIILIILQLLFSLITSNLSYSSLIKELARTGVNWK
                     WQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWIVTF
                     IFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     misc_feature    complement(4068..4086)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.94"
                     /tool="antismash"
     gene            4302..4364
                     /locus_tag="NW953_RS04595"
                     /old_locus_tag="NW953_04595"
     CDS             4302..4364
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /locus_tag="NW953_RS04595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04595"
                     /product="hypothetical protein"
                     /protein_id="WP_228764722.1"
                     /transl_table=11
                     /translation="MFIVTFIGIVVAIINLSNKK"
     misc_feature    4933..4947
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     gene            5001..5285
                     /locus_tag="NW953_RS04600"
                     /old_locus_tag="NW953_04600"
     CDS             5001..5285
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790905.1"
                     /locus_tag="NW953_RS04600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04600"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000790905.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.3e-24,
                     bitscore: 82.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNKIFGAILYLSLALGLSTAAYASTEYAEGGTWSHGVGSKYVWS
                     YYYHGHKGHGATAIGKYRSFSGYTRAGVKAKASATKNNWWVNRAYYNIY"
     gene            5329..7293
                     /locus_tag="NW953_RS04605"
                     /old_locus_tag="NW953_04605"
     CDS             5329..7293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /locus_tag="NW953_RS04605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04605"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_258409829.1"
                     /transl_table=11
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDWN
                     KKYNKNEIYKHINQFARNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKYNI
                     HYLSDDELLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMN
                     IVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIITQ
                     ILLTTSVLWILNYTGNLDLFILRLVLLSCLFILTISVINLWTFLMLLNLNIANMIKGKQ
                     HFKTIRFINTVCKSILLVLIASVMIENTSVIKDLNKIKETEKYWNILDDYYTIEFAPYH
                     ETKQSLIDNMLRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIY
                     HKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSLQFIN
                     KNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNI
                     EKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQH
                     RKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILHSHYIMMIFATIIVVQILL
                     QICSLYYHGRRFNEVIKEF"
     gene            7296..7616
                     /locus_tag="NW953_RS04610"
                     /old_locus_tag="NW953_04610"
     CDS             7296..7616
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /locus_tag="NW953_RS04610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04610"
                     /product="YxeA family protein"
                     /protein_id="WP_258409830.1"
                     /transl_table=11
                     /translation="MKYIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVPK
                     GTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISIKYVEKDDTPKEVR
                     QE"
     gene            7613..8254
                     /locus_tag="NW953_RS04615"
                     /old_locus_tag="NW953_04615"
     CDS             7613..8254
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /locus_tag="NW953_RS04615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04615"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000571182.1"
                     /transl_table=11
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLLN
                     TIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYKKI
                     SKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPTGAL
                     DPETGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASL"
     gene            complement(8342..8632)
                     /locus_tag="NW953_RS04620"
                     /old_locus_tag="NW953_04620"
     CDS             complement(8342..8632)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /locus_tag="NW953_RS04620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04620"
                     /product="hypothetical protein"
                     /protein_id="WP_001796515.1"
                     /transl_table=11
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYAAS
                     ALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILLFFSLPLLLS"
     gene            8722..8910
                     /locus_tag="NW953_RS04625"
                     /old_locus_tag="NW953_04625"
     CDS             8722..8910
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /locus_tag="NW953_RS04625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04625"
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="WP_072399158.1"
                     /transl_table=11
                     /translation="MEDNKNGCLIDNHDINDMADKILKLVNHDVLAEEFGSKARENIIE
                     KYSTESILEKWLNLFNS"
     gene            complement(9000..9356)
                     /locus_tag="NW953_RS04630"
                     /old_locus_tag="NW953_04630"
     CDS             complement(9000..9356)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /locus_tag="NW953_RS04630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW953_04630"
                     /product="DoxX family protein"
                     /protein_id="WP_000766009.1"
                     /transl_table=11
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIAV
                     LMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMNLA
                     CISSYSWKKWLQFY"
     misc_feature    10133..10148
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.2"
                     /tool="antismash"
ORIGIN
        1 aaggggttga gtggacagat tcgaaatgaa gatttgtatc atgcataccc atatccagat
       61 aagcccatgg atatgacaat tagtggtcaa aatatcaaag atatattgga gtatagttat
      121 tcacatttag attttgttaa cgagcaatta agcttaacaa ttattgatga aacattatgt
      181 acaatgtggc aaggattcaa ttatgagatt gatatgaatc aagaacctgg gcaacgagta
      241 atgttagatc aaattgattt gactaagagt tatagagtta caatgactga ctattgttat
      301 cgtaactaca agaattattt aaaaaatgct attatacatg aatcatacga tgaaacaatg
      361 agtacattaa ttgcagagaa gttaagagat ccgaattatc gtatttcatg tagtgataat
      421 tttgtagtta aaaacaggta aatattgcta tgtaataaat taattataag tttaacaagt
      481 ataaaaacct ttcttagaga agattatcta ggaaaggttt ttagaatttt aaaattgata
      541 taaaagaaca gtgaaattct cattcattat atatataagt aaacaaaaaa gcttaaatgg
      601 tatataccat ttaagcaagt aacggaaacg gagggattcg aaccctcgcg ccgctttcgc
      661 gacctacacc cttagcaggg gcgcctcttc agccaacttg agtacgtttc caatggctcc
      721 acaggtagga ctcgaaccta cgaccgatcg gttaacagcc gatagctcta ccactgagct
      781 actgtggatt aatttgtatg tgtttgaata tcaagcacaa atattattat aacaagatta
      841 agttaatttg caatagatac cttaaaaaaa tatgcagaac atttacacta tatttataaa
      901 acattccgca tattttaaaa ataaacaatt tatttatctt caaataaaga atagcgtgtc
      961 aatgcttcaa acacattcaa tgtggcttta ttataatcta ttggttggtc gggatttttt
     1021 gatatcatgc gagattgttt atctaccatg taatagtaaa aaatggaatt gttatattgg
     1081 tgccataaac tgtggtatga aacatgaaga atgattgatt cattattaat aaatgtcact
     1141 ttacatttac gattttttaa ttctttaata ctttcgatat aatgcatatt taaccaaata
     1201 ttttcatttt gtctgtcaga atgtgttggg aaaaaataag ttggaaataa tggtgttagt
     1261 aaaataggtg gtttactaga aatgccagta atgcggtttg tctctgcttt cttgccaaga
     1321 taagtattac cataaaattt gcaagagcgt tcaatgattt tctggacttt aaaaggattt
     1381 tgaatacgcg ttttgtcaaa ccgaattatt tcactaccat ttctttgatc gtcatcatca
     1441 aatgctggtc gaataaccat gtctcctttg cgtatcacat aaatattttg agaatacata
     1501 agcaaaacct cgctttatta agttttaaac cattaatata aaaatacaat taatgatata
     1561 aagatatttt ataataaaat tctgagttgt caagttatta ttttggtgaa aatgtacaat
     1621 aatttttaac taatagtcag aatattcatt gatttcaaaa ttagaaaaat gtatacttat
     1681 tataaattaa agaaaggtag tggtttctat gaaatacaat actaatgtta aacatacaac
     1741 tttagaagcg tttgtcacaa ccgtcaatga tttgggtatt gaattaatta tcaatgaagc
     1801 acttcgagag gtaagaaaac gacagctcat agaacttata gatgacgcac tcgtcaataa
     1861 agatgaagca gcatttaatc aatatacggc agaatacaaa aatttggagg catttctcgg
     1921 tgaataacat tgattcgaat tctataacgc aatcataaaa ggactattct taacatctca
     1981 tacatgtaag aatagtcctt taatcgttta tgacattaat ctaattaatt catgtctatc
     2041 tatgtcaccg aaatagtgat ataaatcata ttctgatagt gcttcttcta tgtgctcaaa
     2101 gtcgtgtaga cagccaacta atgcattttc aagatcagtg acatctccga caccaaagaa
     2161 atcaccgaaa atttttgcat gttcgatttt acctcgttta acatcaaact tgatttgtac
     2221 aaatcctttt tcaaattttt cctcgcgttc aaagttatat ttagggtttc ttccataatt
     2281 ccattcccaa gttctatatt tgtcgttact taacttttca atattttccc aatcttcatc
     2341 cgttaattta tattcttcta cttcagtttc tccaaagata gttttcaaga tgattttttt
     2401 aaattcttca atttctaaag ggtcatttaa aaattcttga atgtttgcta ctcgtttacg
     2461 aacagattta ataccttttg atttaatctt agctggatta acttttagtg cattctgaac
     2521 ttcgtctaaa tcactattta acatcaacgt accatgacta aacattctat ttttaacttt
     2581 aaccatggca tttcctgaga ttttcgcttg tccaacttga atatcgttac gaccagttaa
     2641 ttcagcattt acgcctaaag attgtaatgc ttgaacaatt ggttcagtga atttttggaa
     2701 attgtggaaa ctgttaccat catcatctgt tataaaacta aagtttaaat tgccagtatc
     2761 atgataaaca gcgccaccac cagaaatcct tctaactaca tcgatgttat gagcatctat
     2821 atatgactga tttacttcct ctatcgtatt ttgattcttt ccaacaataa tagatggtct
     2881 atttatgtaa aataaaaagt aactttcttc tgctggtaaa ttttttaaaa catattcttc
     2941 cattgctaag tttaaagttg gatctgtaat attattatta ctaatgaatt tcattacaat
     3001 ctctccctta tatctatata tattctctac ttatttatgc cttaactttg ctcaaaatgc
     3061 caattatata ctcacaaatc attctaaata tcggcttaaa gttacatttt attatttttg
     3121 attgttaata gtttatttgt taagtgaatt ttagtacaat ataatttagt tatgatttaa
     3181 ggaggactta aaatgactgt tgcagaagtg ggtaacattg ttgagtttat ggatggatta
     3241 agaggtcgtg ttgaaaaaat caacgataac tctgttattg ttgacttaac aattatggaa
     3301 aattttaatg accttgattt accggaaaaa actgttatca atcataaacg atataagatt
     3361 gttgaataag aaggtaagtt ataatgaata aaatctcgaa ggctttaact tggtttatta
     3421 taagtttcat tatatttcat ctcatattat ttattatgtg gggcgaacac caagaatact
     3481 ggtatttata tacaggtata atgctaattg ctggtatcag ttatgtattt tatcaaagag
     3541 atattgaatc taagcggttg cttacatcaa ttggtgttgg tattattacg gcaattattt
     3601 taattatact tcaactttta ttctcactta taacttctaa tttaagttat agttcattaa
     3661 ttaaagaatt agcaagaaca ggtgtcaatt ggaagtggca aatgttagta actttacttt
     3721 ttgtcattcc atgtcacgag ttatatatga gaactgtttt acaaaaagaa ttaacacatt
     3781 tttcaatacc gaaatgggta gctatattaa taactgcaat ttgttctagt tcattattta
     3841 tttatttaga taattggtgg atagtaacat ttatatttgt tgcacaagtg atattatcat
     3901 taagttatga gtatactcga cgtatagcaa caacatccgt tgcacaaatt gtagctatta
     3961 tattgttatt aatttttaat gcttaaataa gcttttctta gacgtatgtg ttctaagaaa
     4021 agcttttttg cttaagaata gcaatacgaa gattcgaggc atgcttcatt atagtgtata
     4081 catttttaac ggcaagcggt tattattgag attaggtttt agtcagtttt tattactaaa
     4141 tattttataa aaatggtata atatttttcg agcaagttga attgatggtg gctatctgag
     4201 taaagggggg tggtgcctat ggcattactt aacacttttg aaaggaaaag cctattgtga
     4261 tatctattgc aaacgcatta catttaatgt taagtttcgg tatgtttatc gtcactttca
     4321 ttggtatagt agtagcaata ataaatttaa gcaataaaaa ataaccatca tttcaaaact
     4381 ttgacataga atgatggtta aagcaaatct catttagtca ccgtcttttt aacgggctca
     4441 ttaggcgaca tgttcctgca tgtcgtcttt ttacattttt atagtaacat atcttttatt
     4501 taattgtcta tttttattag atgtgtaaca aaattttgaa gtattttccg tatattgcct
     4561 gctaaacatg tcagatttga aaggaaaatc atactgtggt agaaatcact tatgattcta
     4621 tggcagtaca tatacataag tgatttctat tgaatatgta atgaagttca tatattaata
     4681 aaactttcat tggtatagta gtcgcaataa ttaatttaaa caatataaaa caaccatctt
     4741 ttcaaaactt tgacaacgaa tgatggttaa agcaaatctc atttagtcac cgtcttttta
     4801 acgggttcaa taggcgacat gttcctgcat atcgtctatt tatgcactgc gcaatttaat
     4861 aataatacaa ttatactttg atgtccattt aacataaaat actggaaatg taaaaaggta
     4921 aattcatctt gaaatattca gaaaattctg tataatttaa ataaagagat atagattaaa
     4981 tataaagagg tgttttttaa atgaagaaca aaatttttgg cgctatatta tatttatctt
     5041 tagcacttgg tttatcaaca gcagcttatg catctacaga atacgcagaa ggaggcactt
     5101 ggagtcacgg tgtcggcagt aagtatgttt ggtcttatta ttatcatggt cataaaggac
     5161 atggtgcaac agctattgga aaatacagat catttagtgg ttatacaaga gctggtgtaa
     5221 aagcaaaagc atcagctact aaaaataatt ggtgggtcaa tagagcgtat tataacattt
     5281 attaaatact aaaaaatcat agaaaataaa caacgcaaga ggtacaacat gaaatggttc
     5341 aaattaatac ttgatgttac aatcttcatt ctaattgcta tattgttatt tgtttataca
     5401 tataaagaaa acgaagaaat attgcctgat actaaatacc ctatagcggt aactgactgg
     5461 aataaaaaat ataataaaaa tgagatttat aaacatataa atcaattcgc tagaaatgag
     5521 aacgtagcaa tctataaatc aacttcaaat tatacaaaca aaaatgtaga taaagatata
     5581 tatgtattta ataaatcaaa agcaacaact atcactcctt ttaatgctaa atataacatt
     5641 cattatttaa gtgatgacga attattaaaa aaagatatca aagggagtta ttttgtaaaa
     5701 gacaaaaatt ttgacgtgtc taaattcata aattttttaa aagaatatgg tgttactgct
     5761 gaatcataca aaatagatca tatgatgatt gccgttggtg tcattaaaca aatgaatata
     5821 gtagttccgt tatcctcact tttaatcgtt tattttattt attatatttt cgaaaagaac
     5881 attaatttca aagcgtatgc gattaagtat ttaaatggtt ttacattaag aaaaataatt
     5941 tttgaaaatt tttcaaagaa atgcacgtat tgggtaacgt taatcataac tcaaattctt
     6001 ttaactacaa gtgtactttg gatattaaat tacacgggta atttagattt attcatatta
     6061 agattagttc tgctttcatg tctttttatt ttaacgatta gtgttattaa tttatggact
     6121 ttcttaatgt tactaaattt aaatattgcc aatatgatta aaggtaagca gcactttaaa
     6181 acaattcgtt ttattaatac agtttgtaaa agtattctct tagtactaat agctagtgta
     6241 atgatagaaa atactagtgt tattaaagat ttgaataaaa taaaggaaac tgagaagtat
     6301 tggaatatat tagatgatta ttacacgatt gaatttgcac cttatcacga aacgaaacaa
     6361 agtttgattg ataatatgct gcgatcagaa caattagtaa aggctagtga agcagaaaat
     6421 aatgcgattt tattcaaacc aaagggtgac tccgttgaca atgacaactt ttcacctgat
     6481 gaggggaatg taatattagt aaataatcag ttttggtcga tttatcacaa gcagtttcaa
     6541 cctgatattc cgataaaaaa tcaaaaaaat aatgtcgaag taattattcc gcaaaagttt
     6601 catgcaatgc gtaatgaaat caatcaagcg tatcattcat ggtttgaatt tgtacaaaat
     6661 aaaaataata aagagaataa gttatcttta cagtttatca acaaaaatga ttatcgaatt
     6721 tttacatttg atgcacgaga tagtcgccat ttgtcattta tagaggcgcc aatcattgtg
     6781 aatgttcagg catcagattt atcgaatgat ttttattatg ccatgatcag tcaaggcggc
     6841 tatttattca aaaattatga cgcgctagta aaaaatattg aaaagtatca tcttgatggg
     6901 gaaataagtg gaataaccaa ttataaagat agcgtgatgg aaatgtatca tgaaaataat
     6961 ttgaaattaa cagtactcaa cttttcacaa atcattatcg caatcatttt aataattatt
     7021 attttatttg atgtgaaata ttattttgaa caacatcgaa aattactcgt aatcaaaaag
     7081 ctatatggtt attcaacatt aagagccaat taccaatact tattaataaa taatatagtt
     7141 gttgttttta ttggaatatt gacgaatgta attttacatt ctcactatat aatgatgata
     7201 tttgcaacga ttattgtcgt tcaaatatta ttgcagattt gcagcctata ctatcatggc
     7261 cgacgtttta atgaagttat caaggagttt taactatgaa atacatcatt gcaatattat
     7321 taggattaat cgtatctatt actattgctt ttacaatcat acatcatcct atacttgatc
     7381 gatttaatcc tttcttaaaa acggaatata gttatgccaa agtgccaaaa ggtacgcaac
     7441 aatatgttaa tattacggct tatagtgaaa gaggggaaaa gcttgattat aaattaacat
     7501 ttaatggatt ttctcctagt agaacgtatg tcgaaataaa gcataaaggg caatatgtta
     7561 tatcgatcaa atatgttgaa aaagatgata caccaaaaga ggtaagacaa gaatgataga
     7621 attaaaagat ttaaccatac aaaaaggtaa tacacatatt ttgaagaaac ttaatttgaa
     7681 atttcaatgt ggaaaatcat atgcacttat tggtaaaagt ggatgcggta aatctacatt
     7741 attaaatact attgctggac ttgaaaaaac aggaaaacaa tatgtctatt ttaatggtca
     7801 attagaacaa tttaaatcta atttttacag agataaatta gggtatttat ttcaaaatta
     7861 tggattaatc gataatctga cagtaaatga aaatttagat attggattag catataaaaa
     7921 aataagtaag aaagaaaaag aacaattaaa gatacgttat atagaacagt ttggtctgtc
     7981 aaacagttta aaaagaaaag ttcatacgct aagtggaggt gaacaacaac gtgtcgcttt
     8041 aattagaatg atgttaaaag atccgattgt tatgttagct gatgaaccaa ctggtgcgtt
     8101 agatcctgaa acaggacaga tgattattca atcattattt gatttggtcg atgaaaataa
     8161 agtgctgatt ttagcaacac atgatatggc tattgcaaat caatgtgacg aaataataga
     8221 tttagaacag tatagaaaag tagcatcttt gtgatatcca aaagataaaa ttaaaagcca
     8281 atcaatggga aatgttgact tcaacactcc gtttgattgg cttttataat gtgttactaa
     8341 gttatgaaag taacaacggc aaactgaaaa aaagcaatat aataatattt aatacgagta
     8401 gtaataaatg taattgattt tttgttttaa tgtagcaaaa aacaatgact aaagatagca
     8461 gcaatatcat gattgcacct gggaagaacc ataatgctga agctgcatac caaatcattg
     8521 gtaacgccat aataatgaaa gatataagac aagaaaggat accaataata ttataagttc
     8581 tagtcaaaat tacacctcca attttcattt taaaatgaca tgcgtaaaac aatcatttta
     8641 aatacatcat acaataaaat taaattgtat gatagatgaa tttgaaatta aattgaattg
     8701 gcttgattaa aaagtgattt tatagaagat aataaaaatg gttgtttaat agataaccat
     8761 gatattaacg acatggctga taaaatactg aagcttgtta atcatgatgt tttagcagag
     8821 gagtttggtt cgaaagcgag agaaaacatt atagaaaaat attcaacgga atcaatatta
     8881 gaaaaatggt taaatctttt caatagctaa atgaaaaaat cctaggtatt catgagtgcc
     8941 taggattttt acgtatgata tatacaaaaa aaagagagga tagtcctctc aaataatagt
     9001 taataaaatt gtagccattt cttccagctg taactactaa tacaagctaa gttcataacg
     9061 atatgtatga tttcaaactc ataaccactt acatatcctc ttgtaagttt aagtgtgaag
     9121 attgagccta acataacaca aatataaaaa acattaacat aaggcgttaa aaagccgata
     9181 ataagtgcta aacctccaac aacttcacca attgacatca atactgcgat ggtaggtggt
     9241 aaacctaaag aacctaagaa atgaattgtt ccatctaaat ctacaaattt caaagtaccg
     9301 tgtactaaga atgatgttgc tagcatccat ctaattaaaa gcatacctaa tttcatcatg
     9361 tcacctccaa catacatttt tttcttaaaa catagtccaa attatatttg taaagatgtg
     9421 gaaagtaaag attatttata aaaataatta ttaaatattt attaaatgtt ctattgtatt
     9481 gaacaattgt tgtagaaaga cgtttaagtc attagttaca ttttgcattt ataaataaag
     9541 attagcacag catagtacaa agtttatagg taaatgaatt aattatggca tcaaaatata
     9601 cgtatcgtta acactgatgt ggaggcttgg tagtagtgag aacaaacaga atgctacttt
     9661 aagcgatatg attaatatta attagtgcgt cattttcaaa attagaggtc atttgctgta
     9721 taggattaga aattttgata atcaactatg ataagaaaaa ataatgacca tttaaatata
     9781 ttataagttt attaaatgat aaaataataa tatagttctc tataaaaaat gtttggaggt
     9841 aaatgtgtga ataggaatat cgttaaacta gttgtgttta tgctaatctt agttgtagca
     9901 gtagcgggtt gtggtcaaaa agatactgaa gagaaaactg atatgacgac aataaaagat
     9961 gaattaggaa ctgaaaaaat taagaaaaat ccgaaacgtg ttgttgtatt agaatatagt
    10021 tttgctgatt atttagcagc attagatatg aaacctgttg gtattgcaga tgatggcagt
    10081 actaaaaata taacaaagtc agtaagggat aaaattggaa catatgaatc ggttgggtct
    10141 agaccacaac cgaatatgga agtgataagt aatttaaaac cggatttgat tattgcagat
    10201 gtcagcagac ataagaaaat caaatcagaa ttgagcaaaa ttgcgccgac aattatgtta
    10261 gtcagtggta cgggagatta caatg
//
