LOCUS       NZ_CP102959            20710 bp    DNA     circular CON 09-JUL-2024
DEFINITION  Staphylococcus aureus strain 12-G-51 chromosome, complete genome.
ACCESSION   NZ_CP102959
VERSION     NZ_CP102959.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2744047)
  AUTHORS   Monecke,S., Roberts,M.C., Braun,S.D., Diezel,C., Muller,E.,
            Reinicke,M., Linde,J., Joshi,P.R., Paudel,S., Acharya,M.,
            Chalise,M.K., Fessler,A.T., Hotzel,H., Khanal,L., Koju,N.P.,
            Schwarz,S., Kyes,R.C. and Ehricht,R.
  TITLE     Sequence Analysis of Novel Staphylococcus aureus Lineages from Wild
            and Captive Macaques
  JOURNAL   Int J Mol Sci 23 (19), 11225 (2022)
   PUBMED   36232529
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744047)
  AUTHORS   Braun,S.D., Diezel,C., Reinicke,M., Linde,J. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2022) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 197x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024741495.1-RS_2024_07_09
            Annotation Date                   :: 07/09/2024 05:50:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,670
            CDSs (total)                      :: 2,588
            Genes (coding)                    :: 2,516
            CDSs (with protein)               :: 2,516
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 43 of 72
            Pseudo Genes (internal stop)      :: 20 of 72
            Pseudo Genes (multiple problems)  :: 21 of 72
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:54:09
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2003758
            Orig. end                         :: 2024468
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102959.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20710
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="6"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..20710
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20710
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10710](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10710
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     misc_feature    814..829
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.52"
                     /tool="antismash"
     misc_feature    872..887
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.44"
                     /tool="antismash"
     gene            1067..2374
                     /locus_tag="NW979_RS09670"
                     /old_locus_tag="NW979_09670"
     CDS             1067..2374
                     /GO_function="GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /locus_tag="NW979_RS09670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09670"
                     /product="TrkH family potassium uptake protein"
                     /protein_id="WP_001045345.1"
                     /transl_table=11
                     /translation="MNKVNKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFIA
                     SSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNRFL
                     IMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTSVSA
                     FNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLHSKLV
                     LTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIASINKS
                     TALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISDKHIKLS
                     IVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYHGITKIII
                     IFVMLCGKVGLLTLLRTFIPPKSPKNYRYTKGQIYL"
     gene            complement(2910..4526)
                     /gene="groL"
                     /locus_tag="NW979_RS09675"
                     /old_locus_tag="NW979_09675"
     CDS             complement(2910..4526)
                     /GO_function="GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /GO_process="GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /locus_tag="NW979_RS09675"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; 60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09675"
                     /product="chaperonin GroEL"
                     /protein_id="WP_000240642.1"
                     /transl_table=11
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFTA
                     PLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEGLK
                     NVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYISEA
                     MEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYILVTD
                     KKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKAPGFGD
                     RRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGDGDENSI
                     DARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIEDALN
                     STRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLE
                     GSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVAAMFLTTEAV
                     VASIPEKNNDQPNMGGMPGMM"
     gene            complement(4602..4886)
                     /gene="groES"
                     /locus_tag="NW979_RS09680"
                     /old_locus_tag="NW979_09680"
     CDS             complement(4602..4886)
                     /codon_start=1
                     /gene="groES"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /locus_tag="NW979_RS09680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09680"
                     /product="co-chaperone GroES"
                     /protein_id="WP_000917289.1"
                     /transl_table=11
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGTG
                     RLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            5062..5805
                     /gene="mroQ"
                     /locus_tag="NW979_RS09685"
                     /old_locus_tag="NW979_09685"
     CDS             5062..5805
                     /codon_start=1
                     /gene="mroQ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /locus_tag="NW979_RS09685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09685"
                     /product="CPBP family intramembrane glutamic endopeptidase
                     MroQ"
                     /protein_id="WP_000197635.1"
                     /sec_met_domain="Abi (E-value: 9e-17, bitscore: 58.6,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIYI
                     QLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIVLT
                     QIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGNRIV
                     AFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNGFVVI
                     FQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(5830..7062)
                     /locus_tag="NW979_RS09690"
                     /old_locus_tag="NW979_09690"
     CDS             complement(5830..7062)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /locus_tag="NW979_RS09690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09690"
                     /product="SdrH family protein"
                     /protein_id="WP_001838024.1"
                     /transl_table=11
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTNL
                     NHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPSPDS
                     KPNPDNPKPKPDPKPDPKPDPDKPKPNPDPKPDPDNPKPKPDPKPDPDKPKPKPDPKPD
                     PDKPKPNPDPKPDPDKPNPNPSPDPDQPGDSNHSGGSKNGGTWHPNASDGSNQGQWQPN
                     GNQGNSQNPTGNDFVSQRFLALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQ
                     NFSGNAYLNGLQQQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDK
                     PDSKQRSFEPHEKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRN
                     KKKNKNESQRR"
     misc_feature    complement(7146..7160)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.53"
                     /tool="antismash"
     gene            7259..7885
                     /locus_tag="NW979_RS09695"
                     /old_locus_tag="NW979_09695"
     CDS             7259..7885
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /locus_tag="NW979_RS09695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09695"
                     /product="nitroreductase family protein"
                     /protein_id="WP_000522384.1"
                     /sec_met_domain="PF00881 (E-value: 9.4e-16, bitscore: 55.6,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHAV
                     KHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSYGT
                     ILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYNPLV
                     DEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            8246..9031
                     /locus_tag="NW979_RS09700"
                     /old_locus_tag="NW979_09700"
     CDS             8246..9031
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /locus_tag="NW979_RS09700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09700"
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="WP_000867951.1"
                     /transl_table=11
                     /translation="MKVQIYQLPIIFGDSSKNETQITQWFEKNMNAEVDVVVLPEMWNN
                     GYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNYQIFNTAFSVNKSGQL
                     INEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPARSGA
                     KIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINPNGDL
                     VGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(9631..9765)
                     /locus_tag="NW979_RS09705"
                     /old_locus_tag="NW979_09705"
     CDS             complement(9631..9765)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF011337.0"
                     /locus_tag="NW979_RS09705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09705"
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="WP_001549197.1"
                     /transl_table=11
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK"
     gene            10001..10564
                     /locus_tag="NW979_RS09710"
                     /old_locus_tag="NW979_09710"
     CDS             10001..10564
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105699.1"
                     /locus_tag="NW979_RS09710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09710"
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="WP_001105696.1"
                     /sec_met_domain="AgrB (E-value: 2.1e-31, bitscore: 105.9,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQIIVGNFFKILV
                     TYSISIFLSVFLFTLVTHLSYMLIRYNAHGAHAKSSILCYIQSILTFVFVPYFLINIDI
                     NFTYLLALSIIGLISVVIYAPAATKKQPIPIKLVKRKKYLSIIMYLLVLILSLIIHPFY
                     AQFMLLGILVESITLLPIFFPKED"
     gene            10567..10710
                     /gene="agrD"
                     /locus_tag="NW979_RS09715"
                     /old_locus_tag="NW979_09715"
     CDS             10567..10710
                     /codon_start=1
                     /gene="agrD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094921.1"
                     /locus_tag="NW979_RS09715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09715"
                     /product="cyclic lactone autoinducer peptide AgrD"
                     /protein_id="WP_001094921.1"
                     /sec_met_domain="TIGR04223 (E-value: 1.5e-09, bitscore:
                     35.0, seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTLVNMFFDFIIKLAKAIGIVGGVNACSSLFDEPKVPAELTNLY
                     DK"
     misc_feature    10586..10595
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            10740..12023
                     /gene="agrC"
                     /locus_tag="NW979_RS09720"
                     /old_locus_tag="NW979_09720"
     CDS             10740..12023
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /gene="agrC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /locus_tag="NW979_RS09720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09720"
                     /product="quorum-sensing sensor histidine kinase AgrC"
                     /protein_id="WP_000447888.1"
                     /transl_table=11
                     /translation="METINNLAMATFQLVILFTVAKFISFVKFNLRDYFIIVGIIIPTM
                     FLYYFYGSRAVLIPTFSSIIFLFFKLKYYAIVTILVTMIIMYLSNFATVGLFLTLRKYT
                     TDPAILLPLYILSFSSVSLLATYLVRISLKKFKKSYLSLNKTYMIIISFVLFATFAFFY
                     IYSTNTSSNGDSLIPYALVFIGLIIFISVVILIMSLFTLKEMKYKRNQEEIETYYEYTL
                     KIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNG
                     IENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEA
                     STEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKE
                     IADNADNVLLDTIIENGFFIQKVEIINN"
     gene            12042..12758
                     /gene="agrA"
                     /locus_tag="NW979_RS09725"
                     /old_locus_tag="NW979_09725"
     CDS             12042..12758
                     /codon_start=1
                     /gene="agrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /locus_tag="NW979_RS09725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09725"
                     /product="quorum-sensing response regulator AgrA"
                     /protein_id="WP_000688498.1"
                     /transl_table=11
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVLE
                     QAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKVAA
                     MDFIFKDDPAELRTRIIDCLETAHTRLQLLSRDNSVETIELKRGSNSVYVQYDDIMFFE
                     SSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKERIVYF
                     KNKEHCYASVRNVKKI"
     gene            12830..12939
                     /locus_tag="NW979_RS09730"
                     /old_locus_tag="NW979_09730"
                     /pseudo=""
     CDS             12830..12939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /locus_tag="NW979_RS09730"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09730"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHCIAGFLIV"
     gene            complement(13127..14086)
                     /locus_tag="NW979_RS09735"
                     /old_locus_tag="NW979_09735"
     CDS             complement(13127..14086)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /locus_tag="NW979_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09735"
                     /product="carbohydrate kinase"
                     /protein_id="WP_103149090.1"
                     /transl_table=11
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTVQ
                     KLGQQAHMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERDFS
                     FYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLIDSPMREAHYQLITKTLNSNGTVVF
                     DPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTGNVTV
                     VIYTKGADGAAVYLKNGNNHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLNLTQLF
                     ENEGEEILAFSNRVAAIVTTKYGAIISLPTLQEVEQAF"
     gene            complement(14083..15567)
                     /locus_tag="NW979_RS09740"
                     /old_locus_tag="NW979_09740"
     CDS             complement(14083..15567)
                     /EC_number="3.2.1.26"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /locus_tag="NW979_RS09740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09740"
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="WP_103149089.1"
                     /transl_table=11
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETGL
                     LNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYDSH
                     GVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQPEGY
                     TSHFRDPKVFKYDEKYYAIIGAQNNNQQGRLLLYNTEDIINWHYLGEINTELDDFGYMW
                     ECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFVELDNG
                     FDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQLKQRPYP
                     ALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTSKTSSTLI
                     AYNKRENKITLDRSDSGLLPANVEGTTRSTILDTPLKQLQIFVDTSSIEIFCNDGERVL
                     TSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     misc_feature    15678..15692
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     gene            complement(15716..16666)
                     /locus_tag="NW979_RS09745"
                     /old_locus_tag="NW979_09745"
     CDS             complement(15716..16666)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /locus_tag="NW979_RS09745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09745"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_258413456.1"
                     /transl_table=11
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDYQ
                     PNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEAEI
                     QALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKAGQI
                     IGEWIGQQGYQQVEVFSVSEKDIAVGIYRKRGLLDQLAKYQIKPNIYETNFTYVEAQKD
                     VANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHQIYGFGGDPMTQLVSPSIKT
                     IHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            complement(16849..18099)
                     /locus_tag="NW979_RS09750"
                     /old_locus_tag="NW979_09750"
     CDS             complement(16849..18099)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008519 - ammonium channel activity
                     [Evidence IEA]"
                     /GO_process="GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /locus_tag="NW979_RS09750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09750"
                     /product="ammonium transporter"
                     /protein_id="WP_061838914.1"
                     /transl_table=11
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSMA
                     AIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQMM
                     FCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFAGGT
                     VVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFTFDNI
                     AMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVTYLSAT
                     IMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKANPDIENG
                     FIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGLDKIVHGE
                     HAYFEGELNRFNKHIRY"
     gene            complement(18307..18531)
                     /locus_tag="NW979_RS09755"
                     /old_locus_tag="NW979_09755"
     CDS             complement(18307..18531)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /locus_tag="NW979_RS09755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09755"
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="WP_000581077.1"
                     /transl_table=11
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEAI
                     PNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            complement(18591..19670)
                     /locus_tag="NW979_RS09760"
                     /old_locus_tag="NW979_09760"
     CDS             complement(18591..19670)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /locus_tag="NW979_RS09760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09760"
                     /product="YeeE/YedE family protein"
                     /protein_id="WP_000266884.1"
                     /transl_table=11
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYALL
                     IAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAGEG
                     LIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILTIIT
                     IFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPMSAST
                     GRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWRLPDKI
                     TIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMSHFIFVR
                     PMKKVQQQSAKVKQQTQIV"
     gene            complement(19974..20609)
                     /locus_tag="NW979_RS09765"
                     /old_locus_tag="NW979_09765"
     CDS             complement(19974..20609)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /locus_tag="NW979_RS09765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NW979_09765"
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="WP_001283612.1"
                     /transl_table=11
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQID
                     SATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTYNF
                     SIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQKVA
                     DELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
ORIGIN
        1 aaaaacatga tactaaacat gaattagatg ttgcacccat gttcaaatca ttgataggaa
       61 aagaaatttc tgaagaggat gcaaattatg catctggtct tacagctgta tgttcgatta
      121 taaatggcgg caaacaattt aactctgtac cagatgaagc ttcacttgaa tttaacgtaa
      181 gaccagttcc tgagtatgat aacgacttta tagaatcgtt tttccaaaat atcattaata
      241 atgtggatag caataagctt tcactcgata ttccaagcaa tcaccgacct gtaacaagcg
      301 ataaaaatag caaattaatt actacgatta aagatgtagc ttctagttat gtagacaaag
      361 acgatatatt tgtttctgcg cttgtaggtg caacagatgc ctctagtttc ttagttgata
      421 ataaggacaa tgttgattta gccatttttg gaccaggtaa tccattaatg gcacatcaaa
      481 tcgatgaata tattgaaaaa gatatgtatc tgaaatatat tgatattttt aaagaggctt
      541 ccattcaata tttaaaagaa aaataagaac gatgctgtca gctgccctat tcgcgtgctg
      601 gcagtttttt attgcgaatt ttaagttgat agaaatagcg atgaatattg tttgactgta
      661 aatattcaaa tatcaacaag cacattttca ttgattaagt gatgtaaaac tgaaattatg
      721 gtggtgattt gtcatacata tattgcattg tctattgaaa ttgggaatcc aatttctctt
      781 tgttgaggcc cccaccccaa cttacattgt ttgtagaatt tcttttcgaa attctctgtg
      841 ttggggcccc caccccaact tgcattgtct gttgaatttc ttttcgaaat tctctgtgtt
      901 ggggccccac cccaacttgc attgtctgtt gaaattggga atccaatttc tctgtgttgg
      961 ggcccctgac tagaattgaa aagtaatacg catcagtaac atttctattt tcatttatga
     1021 tattatctaa ttattaattt ataatataaa ggagtatgca attaaaatga acaaagtcaa
     1081 caaaccttta tatttttatt taatgctttt cttctctaca actatcatag gtgctctttt
     1141 attatattta cctttcactg gtaaaaagcc aatatctttt ttagatgccc tatttatagc
     1201 ttcaagtgca tttacagtta ctggcctatc cccagttgat ataggatctc agtttaatat
     1261 acttggtgaa atagtaatac tattattaat tcaaataggt ggtctgggta tcgtgaccgt
     1321 aaccctattg acactagtat ttttaaatag aaagatatca atgaaaaata gattcttgat
     1381 tatggttaca tggaatattg acgaacctgg tggtgttatt aagctaatta aacacttggc
     1441 tatttatagt ttagtcactg aattaattgg tatgatttgt ttgtgtttat cttttatacc
     1501 aaaatttggt ataggcaaag gtttattttt aagcttattc acatcagtat cagcttttaa
     1561 taatgctgga tttgcccttt ttaagaataa cttaatagat tattctagtg atccaattgt
     1621 cattattaca atctcaatac tcataatatt tggaggtatt ggacattttg tcgtgataga
     1681 ctttattaat tgtaaaaaat tgagtaaatt atctttacat tctaaattag tcttaactac
     1741 aactagtatc ctaataatta taggagctat tacattcttt ttattagaac agtttaatac
     1801 tatgcaacat atgggactag ttgaaaaaat cggaaattct tttttccaat cagtaacaac
     1861 acgaacagcg ggttttaaca gtatagatat agcaagcatt aacaaatcta ccgcattaat
     1921 gttaatgcta cttatgttta ttggtggtgc ccctctcagt gcagctggag gaattaaaat
     1981 aactactttt gcagttgcgt ttatttttgt actaaattat atacgtaaag aaaataatgt
     2041 ttcagtattc aataaagaaa tatctgacaa acatataaaa ctatctattg ttaccattaa
     2101 tatctcattt ctatttatca gcatcattac ttttatatta tcgataatta atccgaacat
     2161 atcattaatc aagttattat tcgaagtggt ttctgcattc ggaacagtag ggttaagtat
     2221 gaaccttacc acagaatatc atggtattac taaaataatt attatattcg ttatgctttg
     2281 cggtaaagta ggactattaa ctttattaag aacatttata ccaccaaaaa gtcctaaaaa
     2341 ttaccgctac actaaaggac aaatttatct ataaaataac tatccatctt taataagaaa
     2401 tgaactagtg ttttaatcta ttacattaaa aaaactacct aaacaggtag ttttttattt
     2461 attttaggcc gattaaatat cttatgtctt taattaagta aaatgcacaa gtacaccttt
     2521 aaaaagatct actcaaaact aaactatacc tagttaaccg cttgtgatta agaaatttta
     2581 aatgtggttt cttttaatta tgtcaaccac ccataaggat tttaatcaag cactcattca
     2641 ttcctgcact cgcaatatgc attacttata gaagtggctg cactctcata ttatggcaat
     2701 accataactc ccgcaccata atgatttaaa taccattatg aatctcaatt acaaattagc
     2761 acataataaa gacacctctc ataagtttag tcaactaatg gaaggcgtct cgtaattaaa
     2821 atatatttac gtgcattata atttgtactt agaatgaatc atactcgtgc ataatgtaat
     2881 tttctagtta gtcagaacta taaacagttt tacatcattc ctggcatgcc acccatgtta
     2941 ggttggtcat tatttttttc tggaattgat gctacaaccg cttcagtcgt taagaacatt
     3001 gctgcaacac ttgctgcatg ttgtaatgct gagcgtgtta ctttagttgg atcaacgata
     3061 cctgcttcta acatattaac ccactcgttt gtagcagcgt taaaaccaac acctggttct
     3121 gcatttttca agcgttcaac gatgacagag ccttctaatc ctgcattttc agcaatttgg
     3181 cgaactggtg cagttaatgc tttaagtaca atattcacac ctgtttcaat gtcgccttca
     3241 gcttcaattt cacttacttt ttggtaaaca tttactagtg cagtaccacc acctgcaaca
     3301 ataccttctt caactgctgc acgtgtagag tttaaagcat cttcaatacg taatttacgt
     3361 tctttaagtt ctgtttcact tgctgcacct accttgataa ctgcaacacc gcctgctaat
     3421 ttagctaagc gctcttgtaa tttttcacga tcaaagtcag attcagtttc ttcaatttga
     3481 gatttcaatt ggctaacacg tgcatcaatg ctgttttcgt caccgtcacc atcaacaaca
     3541 gtggtattat ctttagttac ttctacttta cttgcagtac ctaacatatc aattgatgca
     3601 tcttttaaat ctaagcctaa atcatcagta atcacttgcg caccagttaa aatagctaaa
     3661 tcttcaagca tcgcttttct acgatcacca aaaccaggtg cttttactgc aacagctgta
     3721 aatgtaccac gcatgcggtt tagcacgata tttgttaacg catcgccttc aacttcatca
     3781 gctacaatta agattggacg attagattga accacttgtt ctaataaagg taagatatct
     3841 tggaaagacg agattttctt atctgttact aaaatgtatg ggcgttctaa ttcagcaacc
     3901 attttatctg aatcagtaac catatacggt gattgataac cacgatcaaa ttgcatacct
     3961 tcaaccactt ctagttcagt gtttagtcca tttgattctt caattgtaat gacaccatcg
     4021 ttacctactt tttccatagc ttcagaaata taacgtccaa tttcttcatc tgctgctgaa
     4081 atcgcaccta cttgcgctat ttcattttta ttttcaactt tttgagaatt ttcatgtaac
     4141 gcttcaacag caactttaac tgctttgtcg ataccttgtc gtaaaccaac tgggttcgca
     4201 ccacttgtaa catttttcaa gccttcttga atcattgctt gagctaatac tgttgcagtt
     4261 gtcgtaccgt caccagcaat ttcatttgtc ttatttgcaa cttcttgaac tagtttagcc
     4321 cccatatttt catatggatc ttctaattcg atttctttag caatcgttac accatcgttc
     4381 gtaattaaag gtgctgtaaa ctctttatct aatacaacat tacgtccttt aggaccaatc
     4441 gttactttaa ctgcatttgc taattggtct acaccacgta acattgcttg acgtgcatct
     4501 tcagagaatt tcaattgttt aaccataaat gataaacctc catttgttta gtcatatttc
     4561 attttcgttc tttacaattt atctatgaat ttagttttat attattcaat taccgctaaa
     4621 atatcttctt catttaatac tagatatgtt tcattatctc gtttaacttc tgtaccagca
     4681 tattgttgga acacgacacg gtccccttct ttcacttcag gagtcactct tgtaccatca
     4741 tttaataggc gtcctgttcc tactgcaacg ataacgcctt cgtttgattt ttctttagca
     4801 ctatcagtta aaacaatacc acttttagtt gtttgttctt gttctttttt ctcaataatc
     4861 acacgatttc caattggttt tagcatgatt gttcctcctt aaaaaaacct aaagtttagc
     4921 acttaacatt aaagagtgct aacatacatt tataataatc aaatttggtc aaaatttcaa
     4981 gtcataacct tttaattaag ttttgtaact tcaagttatt tacgataaaa taaattataa
     5041 acaaatattt ggaggaaaat tatgacaaga ctatgggcat cattgctaac tgttattatt
     5101 tatatattgt ctcaattttt accgcttctc attgtaaaaa aattaccatt tgtacaatat
     5161 agtggcatag aactgactaa agcagtcatt tacatacaac ttgttctatt tttaatcgcc
     5221 gccacgacga ttattttaat taatttaaaa attaaaaatc caacaaaatt agaattagaa
     5281 gttaaagaac ctaaaaaata tatcattcca tgggcattgc ttggatttgc attggtaatg
     5341 atttatcaaa tggtagtgag cattgtatta acgcaaattt atggtggaca acaagtaagt
     5401 cctaatacag aaaagctaat tattattgct cgaaaaatac ctatatttat cttctttgta
     5461 tctattattg gtcctttatt agaagaatac gtattcagaa aagtaatctt tggagaatta
     5521 tttaatgcga ttaaaggtaa tcgtatcgta gcatttatta ttgctacaac agtaagttca
     5581 ttaatatttg cattagcaca taatgatttc aaatttattc cagtttattt tggtatgggt
     5641 gtcatttttt ctttagcata tgtatggaca aaacggcttg ctgttccaat tattatccat
     5701 atgttacaaa acggatttgt cgttatattc caattactta atcctgaggc tttgaaaaaa
     5761 gccacggaac aagcgaattt tatatatcac atttttattc cataatataa aaaacgaatc
     5821 acacacggat tatcgtcgct gtgattcgtt tttatttttc tttttatttc gctttacaaa
     5881 taagaatagc atgattgaaa ataccaccat catagaacat acaattgcta gccaactttt
     5941 actatatgca gttgacgcac ttttcttatt atcttcttgt tttttaacta ctgtaaaatc
     6001 gtctttttca tgcggttcaa atgagcgttg ttttgaatcc ggcttatctt cgggcttttt
     6061 caaatctggc attgatccaa tttcaccagt aattaatgcg agtacttgtt catctaaatt
     6121 acgatagtac ctttctgatt tcaatggatt gaaatattgg aatctaaagt aattcgattg
     6181 ctgctgtaat ccatttaaat atgcatttcc gctgaaattt tgttttcgaa taatattata
     6241 aatgtcttca tcagtaactt ctccataatc tttgcccaac ttattaattt gatttaaaat
     6301 atacggatta tacttgtaag ccccatttgc caaggctaaa aatcgttggg atacaaaatc
     6361 attaccagta ggattttgtg agtttccttg attcccattt ggttgccatt gaccttgatt
     6421 agatccatct gaagcatttg ggtgccatgt tcccccattt ttcgagccac cagaatgatt
     6481 ggaatcccca ggttgatcgg gatctggtga cgggttagga tttggcttat ctggatctgg
     6541 ttttggatct ggatttggct ttggcttatc tggatctggt tttggatccg gttttggctt
     6601 tggtttatct gggtctggtt ttggatctgg ttttggtttc gggttatctg gatctggttt
     6661 tggatccgga tttggctttg gtttatctgg gtctggtttt ggatccggtt ttggatccgg
     6721 ttttggtttc gggttatctg gatttggttt tgaatctgga cttgggtttt ggttgtttga
     6781 gtctggcttc acattattcg aatcaggatt agcatcacga ctattcgaac ctgttccttc
     6841 attacccgtt tgatttgatt cgtgaggtgt cccagtctca ttattattca tttcactatt
     6901 tactgatggt gaagttatat tatgattcaa attagtagtt ggtttttctc catttaagtt
     6961 atcctttgct tcaactgtgt gcgttgcaag ccctaaacta ctactactta aaattaaaat
     7021 acttgttgaa agtaacattt tcttaaacca atgatatgac atttcacgag acacctccct
     7081 ttttcatgta agtattaatc taagtataat aaatatgcgt tgaaaatgca tttatatttg
     7141 gaatttgaaa atgaatatta atattcttga aattttcgta aatattactt tacaagttca
     7201 ggtataaatt ttatacttag tctattaagt attctttact aaagaaggag caataattat
     7261 gggcttattt aaaaaagata aaaaagcaat gacttttgac aatgcaatgg aagaaagacg
     7321 ttcaatatat aatttgaaag attcgatatc aattagcgat gacgaattag aatcaatgat
     7381 tgcacatgca gttaaacatg ttccttcatc atttaattct caatcaacta gaattgttct
     7441 cttgttaaat gacaacaata ataaattttg ggataacaca aaagcaattt taaaagaagt
     7501 catgggagag aatcgtgatt tcgagccaac tgaacaaaaa attgataact tcaaacattc
     7561 atacggtaca attttgtttt atgaagatca agatgttgta agtggtttac aagaacaaat
     7621 gccaaactac tatgataatt ttgcaatatg gtcaactcaa acaaatgcca tgcatcaatt
     7681 tgcaatttgg actgcattag ctactaaagg cataggtgct tcattacaac actataatcc
     7741 acttgtcgat gagatgactt caaatgaatt taatattcct aaaagttgga aacttattgc
     7801 tcaaatgcca tttggtgata ttcgtgaagc tgctggcgaa aaaacattta atccagtgga
     7861 agatcgtttc gtaataaaaa aataatcaat atattgttaa gaatgggaca gaaatgataa
     7921 agaatcatta gttattcatt atgtagtagt tcttacacat tagccgctgc taatgtgcac
     7981 ttaagaatag gaatacatga gtaaaactca tgtataagaa atactaattt ctaaagaaaa
     8041 agtatttctt tatgttagag cccaccccaa cttgcattgt ctgtagaaat tggtgttcca
     8101 atttctctat gttggggccc catcaactac tgtcaatata acattgtaga gcctaggact
     8161 ttgattttat gtcttgggct cgttttttgc gtttcaacat aacttttagt aagttatata
     8221 taagatctat caaggatgtg atgttatgaa agtccaaatt tatcaattac cgattatttt
     8281 tggagatagt tctaaaaatg aaactcaaat aacgcaatgg ttcgaaaaaa atatgaatgc
     8341 tgaagtagat gtagtcgttt tacctgaaat gtggaataat ggctatgatt tagagcattt
     8401 aaatgaaaaa gcagataata atctaggaca aagtttttca tttattaaac atttagctga
     8461 aaaatataaa gtcgatatcg tagctgggtc agtttctaac attagaaact atcaaatatt
     8521 taatacagct tttagtgtga ataaaagtgg ccagctgatt aatgaatacg acaaagttca
     8581 tttagttcct atgttaagag agcatgaatt tttaaccgct ggcgaaaacg tagctgaacc
     8641 ctttcaattg tctgacggta cttatgtgac ccaacttatc tgttatgatt tacgttttcc
     8701 agaattatta agatatcctg ctcgtagtgg tgctaaaatt gcattttatg tggcgcaatg
     8761 gcctatgtca cgtcttcaac attggcattc attactaaag gcacgtgcaa ttgaaaataa
     8821 tatgtttgtc attggaacta atagcactgg attcgatggt aacacagaat atgctggtca
     8881 ttcgatagtt ataaacccaa atggagattt agttggagaa ttaaatgaat ccgcagatat
     8941 tttgactgta gatttaaact taaatgaagt agaacagcaa cgcgaaaata tacctgtatt
     9001 taaaagtatt aaattagatt tgtataaata agaagcgccc gaaataatat ttaacacatg
     9061 tcatgactaa acatagattt atgagagatt tttcataagt ctatgtttat ttttttataa
     9121 tagataactc agtaagaatc catttcgccc tttgcaaatg aatgattttc ttaattaaat
     9181 tgattaattt tttgaccttt tccaacatta gacttattca tatattttaa cggtgggtct
     9241 cataatgagg attaactctc cccttcttta ttacaataaa aaaggccgcg agcttgggag
     9301 gggctcacga ccatacttat tattaaggga atgttttaca gttatttttt caatctattt
     9361 ttggggatgt tattaattat gaaaaaaatt ttatttatca aataatgata aatcgatgtt
     9421 gtttacgata gcttacatgc tagaaataat tatctgtaat gataattaag aaaaatacat
     9481 agcactgagt ccaaggaaac taactctact agcaaatgtt actcacttgc taattttaca
     9541 tggttattaa gttgggatgg cttaataact catactttta gtactatacg aagataacaa
     9601 atttacaatg aaagtaatta attattcatc ttatttttta gtgaatttgt tcactgtgtc
     9661 gataatccat tttactaagt caccgattgt tgaaatgata tcttgtgcca ttgaaatcac
     9721 tccttcctta attaagataa aaattcttaa aattaaacaa ctcatcaact attttccatc
     9781 acatctctgt gatctagtta tattaaaaca tgctaaaagc atttattttc gattttttct
     9841 taactagtcg ttttttattc ttaactgtaa atttttttat gttaaaatat taaatacaaa
     9901 ttacatttaa cagttaagta tttatttcct acagttaggc aatataatga taaaagattg
     9961 tactaaatcg tataataaca gtgaggagag tggtgtaaaa ttgaattatt ttgataacaa
    10021 aattgaccag tttgccacgt atcttcaaaa gagaaataac ttagatcata ttcaattttt
    10081 gcaagtacgt ttagggatgc aaattatagt tggtaatttt tttaaaatac ttgtaacata
    10141 tagtatttct atattcttaa gtgtattttt gtttacctta gttacacact tatcctatat
    10201 gttaatacgc tataatgcac atggtgctca tgcaaagtct tcgatacttt gttacataca
    10261 aagtatttta acatttgtat ttgtacctta ttttctaatt aatatagaca ttaattttac
    10321 ttatttactg gccttatcta ttataggatt gatttccgtt gttatttatg cacctgcagc
    10381 aacgaaaaag caacctatac caataaaatt agtaaaacgt aaaaagtatt tatctataat
    10441 tatgtattta ttagttttga ttctatcctt aataatacat ccattttatg ctcaatttat
    10501 gttattagga attttagtag aatctattac attattacct atttttttcc ctaaggagga
    10561 ttaatcatga atacacttgt taatatgttt tttgatttta taattaaatt agctaaagca
    10621 atcggaattg tcggtggcgt aaacgcttgc agcagtttat ttgatgaacc taaagtaccc
    10681 gctgaattaa cgaatttata cgacaaatag taaaagttat tttagaaagt gtgtagcata
    10741 tggaaacaat aaacaactta gctatggcca cttttcaatt agtaatactt tttacagtag
    10801 ccaaatttat ttcctttgtt aaatttaatt taagggatta ttttattata gttggaatta
    10861 taattccaac catgtttttg tattacttct atggaagtag agccgtattg attccaactt
    10921 tttcatctat catattttta ttctttaaat taaagtacta tgcaatagta acaattttag
    10981 tgaccatgat cataatgtat ttgagtaatt ttgcaacagt aggtttgttt ttaactttaa
    11041 gaaaatacac tactgatcct gcaatactct taccattata tatactttct ttttcatcag
    11101 tttcattatt agcgacatat ttggtaagaa tatctctaaa gaaatttaaa aaatcatact
    11161 tatctttaaa taaaacttat atgattataa tttcattcgt attatttgca acatttgcat
    11221 ttttttatat ttattctaca aatacaagtt caaacggtga tagcttaatt ccatatgcac
    11281 ttgtatttat aggattaata atttttatct ctgttgtaat acttataatg tcgcttttca
    11341 cccttaaaga gatgaaatac aaacgaaacc aggaagaaat tgaaacctat tatgaatata
    11401 cattgaagat tgaagctatc aacaacgaaa tgcgcaagtt ccgtcatgat tatgtcaata
    11461 tcttaacgac actttcagaa tacattcgcg aagatgacat gattggtcta cgcgcttatt
    11521 tcaataaaaa tattgtacct atgaaagata atttgcagat gaatgctatt aaattaaatg
    11581 gtatcgagaa tcttaaagta cgtgagataa aaggcttaat tactgcgaaa attttacgtg
    11641 cacaagaaat gaatattccg attagtatcg aaatacccga tgaagtaagt agcattaact
    11701 tgaatatgat cgatttaagt cgcagtattg gtattattct tgataatgca attgaggcat
    11761 caactgaaat tgatgaccct atcattcgcg ttgcatttat tgaaagtgaa aattcagtaa
    11821 cgtttattgt tatgaataaa tgcgctgatg atataccacg cattcatgaa ttgttccaag
    11881 aaagtttttc tactaaaggt gaaggtcgtg gtttaggtct atcaacttta aaagaaattg
    11941 ctgataatgc agacaatgtc ttattagata caattatcga aaatggtttc tttattcaaa
    12001 aagttgaaat tattaacaac tagccataag gatgtgaatg tatgaaaatt ttcatttgcg
    12061 aagacgatcc aaaacaaaga gaaaacatgg ttaccattat taaaaattat ataatgatag
    12121 aagaaaagcc tatggaaatt gccctcgcaa ctgataatcc ttatgaggtg cttgagcaag
    12181 ctaaaaatat gaatgacata ggctgttact ttttagatat tcaactttca actgatatta
    12241 atggtatcaa attaggcagt gaaattcgta agcatgaccc agttggtaac attattttcg
    12301 ttacgagtca cagtgaactt acctatttaa catttgtcta caaagttgca gcgatggatt
    12361 ttatttttaa agatgatcca gcagaattaa gaactcgaat tatagattgt ttagaaactg
    12421 cacatacacg tttacaatta ttatcaagag ataatagcgt tgaaacgatt gaattaaaac
    12481 gtggcagtaa ttcagtgtat gttcaatatg atgatattat gttttttgaa tcatcaacaa
    12541 aatctcacag actcattgcc catttagata accgtcaaat tgaattttat ggtaatttaa
    12601 aagaactgag tcaattagat gatcgtttct ttagatgtca taatagcttt gtcgtcaatc
    12661 gccataatat tgaatctata gattcgaaag agcgaattgt ctattttaaa aataaagaac
    12721 actgctatgc atcggtgaga aacgttaaaa aaatataata agataataaa gtcagttaac
    12781 ggcgtattca attgtaaatc ttgttggatt ttaacaagat aactagcaaa tgcactgtat
    12841 agctggcttt ttaattgttt aataacaaaa ttaaatatga cgcgtgaatt aaaaaatgat
    12901 gtaacgtctc tttgtatacc tattcctatt actacataat ttatcaattt ctatttcact
    12961 ctacaatcct atggcgcaat ttttaacaaa atatactaaa tttgaaatcg acagtttcct
    13021 taaataaaat actacaattt cctaccccat gaatcttatt ttgttttaaa aaacagtgta
    13081 gcacaagatt caacttgttt cctacactgt tttataaatg aattatttaa aatgcttgtt
    13141 caacctcttg taatgttggc aaactaataa tggcaccata cttcgttgtc actattgcag
    13201 caacgcgatt gctaaacgct aaaatttcct caccttcatt ttcaaataac tgagttaaat
    13261 ttaacacatc ggttgctaaa atcctgctaa taactgcacc aataaaagca tcccctgccc
    13321 cagttgtatc aacaggtttt accttatagc cactatgata atgattatta ccatttttca
    13381 aatatacggc agcaccatct gctcccttcg tataaatgac tacagtaaca ttacctgtaa
    13441 ataatgattg aatggcttca ttttcatcat gaatacctgt aataaattct aattcttcat
    13501 ctgaaacttt tacaatatgc gccaatggca aaaaggtatg aatagtttgt cttaaatctt
    13561 cagcgttatc ccataatggc aatcttacat taggatcaaa tacgacagta ccatttgaat
    13621 ttaatgtttt ggtaatcaat tgataatgtg cttctctcat cggactatcg atcaaatcaa
    13681 cagaacaaaa atgtaccaca tcattttcat tcacatcaat atcattcaca aaacttggtt
    13741 caaataacat atcagcagag ggcttacgat aaaaagaaaa atctcgctcg cccgcttcag
    13801 tcaaacttac aaaagctaac gcagtgttgg cttcattcgt gcgatacact tttgatacat
    13861 ctacaccaat acttgatatc gtttcaataa tgctatctcc aaatgcatca ttacctaatt
    13921 gtgtaatcat atgcgcttgt tgtcctaact tttgaactgt acaagctaca ttgcatggcg
    13981 cgccaccaat ttgcttcgta aatgtttgaa catcttttaa attcgcattt gtaatgtttg
    14041 gaataaaatc aattaacgct tctcctattg aaaatagacg tctcatttat gttcatcctt
    14101 taaatcatat ttagtaaatt gtaaatatac ttgccctgat tctgttgatg ttttaatacc
    14161 tagtgcgtct tctgtcggga aaattcgaga tgtcaaaaca cgttccccat cattacagaa
    14221 aatttctata ctagatgtat caacaaaaat ttgtaattgc tttaatggcg tgtctaatat
    14281 cgtactacgc gtcgtacctt caacatttgc cggcaataaa ccactgtcgc tgcgatctaa
    14341 tgttatttta ttttcacgct tgttataagc gattaatgtt gaagaagtct tagatgtacg
    14401 taattcaaag tacacttcgg tagcatcatt atccaaaata tctatgatta attcatactg
    14461 tttaccttca tacggatgta attttcgagt aaatttattt gcgtagccta atgctgtctc
    14521 tttattgtga cgtaattttt ccaacgctgg atatggacgt tgcttaagtt gaccattttc
    14581 tacatttaat acacgaggaa ttgttaagca atgggcccat ccttcattat cagtaggata
    14641 ttcgatttcc ggtaacccca tccatccaat tagtactcgt cggccttttt catctaaaaa
    14701 tgtttgaggt gcatagaaat caaagccatt atcaagctcg acaaaatttt catgttcata
    14761 tgttaacttt tcaatatcaa actttccaag tatataacca ctttgataaa tatttttgaa
    14821 ctgatcgcct tttggttcaa taccttgtgg acaaataagt atgacatctt gattatctac
    14881 attaaagtaa tctgggcatt cccacatata tccaaaatca tccaactctg tatttatttc
    14941 acctaaataa tgccaattaa ttatatcttc agtattataa agtaataatc gaccttgctg
    15001 attattattt tgtgcgccaa tgatcgcata atatttctca tcatatttaa aaactttagg
    15061 atctctaaaa tgacttgtat atccttctgg ttgctggcta attactggct ttggaaactt
    15121 ttcaactgaa ccgtcttctt tcagtcgtgc gatcatctga ctcgcatgtc gttgccaatg
    15181 attatctcga tgatttcctg tgtacatata atataaatgc ccgttatatt caaaagcact
    15241 tccgctataa acaccatggc tgtcatattt agtatctgga tttaatattg gcccttcagc
    15301 tttaaagttt attaagtcat cactcgtgta gttataccaa tactttaagc catgtactgc
    15361 gcctaatggg aaccattgat gtgaaacata atacttccct ttataaaaaa taagtccgtt
    15421 ggggtcattt aataagcctg tttctggttg tatatgaaat tgttgacgaa attttgattg
    15481 atcaacttgt tgttttaatg ttttaaaata ctcagtatca acgtcctcga ttcgttgata
    15541 acgttcttct ctagtccatt cggtcataat atttacccag tcctcctttt ataatttatg
    15601 ctgttaacaa tttatattct attatagcaa attttaccta cgttctttaa cttttaacct
    15661 atccatttat agttatatgg tatcggttcc acatttaatt taaaaaatac agcgtctaaa
    15721 tattaacatc tactgtgacg ctatatggca tatcttgctt tttaagcatc tgttgtatct
    15781 cttccatcgc gcattgacca gcttcaaaat aattataatg aattgttttt atcgatggag
    15841 acactaattg tgtcattggg tcaccaccaa aaccatatat ttgatgtggt ttcataacat
    15901 cttttttatc agaataatat ttataggcag ctaatgcaat cgtatcagtt gctccaacaa
    15961 ccgcatctac ttgctccaca ttttccaaaa catttgcaac atctttttgt gcttccacat
    16021 aagtaaaatt tgtttcatat atattaggtt taatttggta tttagctaac tggtcaagta
    16081 aaccacgttt tctatatata ccaactgcaa tatctttttc acttacgcta aacacttcaa
    16141 cttgttgata tccctgttga ccaatccatt cgcctataat ttgacctgct ttataatcat
    16201 catgcacaat actatgaagt tgttcatgtt gttgaccaac aataacgatt ggtacattca
    16261 ttttattaat gacttctata tgtctctctg ttatatctgt agccattaaa acaataccat
    16321 ctactttact gcgtgctaat gtttcaagcg cttgtatttc tgcttcgata tttaaacctg
    16381 tataatttaa aattaattgt gattcatatt tttggcattg ttttgccaat cctttgattg
    16441 tttcgtctac tgcatatgaa ttcattctag gtataatggc accaataagg tgtgtttgtc
    16501 tcgctcttaa actttgagca aattgattcg gttgatagtc atgttctgct ataatttttg
    16561 ttaatttttc acttgttttt ttactgacag atccattatt taaaaatcta gatactgtac
    16621 tttttgaaac gcctgccaat ttggcaatat cagatatatt tttcatactt attcacctat
    16681 cattatttgt gacacttagc cttattttag catatgtgta accgctttaa ataatatttc
    16741 tgcttctttc ataaaaagat ttcaaaataa aacttttgta taacaaaatg tcccaacatc
    16801 aacctttagt taaatgttag gacaatgaat attctatgta aatatattct aatatcgaat
    16861 atgtttattg aatctattta gctcaccttc aaagtaagca tgttcaccgt gaacaatctt
    16921 gtctaatcct atattcgttt cttgttccgt aacagataat ggtgtaatta atttaattac
    16981 tttcgcaata ataaacgtca tgacgatact aaaaattaca actgctgtta cacataatat
    17041 ttgtacaagt ataatatgta tgtcaccagt ataaataaag ccattctcaa tgtcaggatt
    17101 ggctttttta ctttggaaaa ctgctgttaa aacagcacca ataataccac caacaccatg
    17161 aataccaaat gcatctaatg catcatgata ttttagtttt accttgatgt aattaatgac
    17221 aatataacaa cagatacctc ctattaaagc cattattgtt gcactaagat atgttacata
    17281 tcctgctgca ggagtaataa caactaatcc tgctaatgca ccgagtaaaa gtccaagtaa
    17341 acttgtcgtc tttttaaaaa tatattctaa aattaaccaa cctatagcac ctgcactggc
    17401 tgaaatgaca gtatttgtaa atgcaagcat cgcaatatta tcaaatgtaa aagcactacc
    17461 tacattaaat ccataccaac caatccacac gaatataccg ccaatcaacg taatgataag
    17521 attatgtggt gttgattcag aatgtttgtt tccttttcca atcataatag ctaatactaa
    17581 accagaaaca cctgatgtaa tatgaacaac cgtacctcca gcgaaatcta atacaccgag
    17641 tttgttaatc caaccgccgc cccaaaccca atgtgctact ggactgtata caagagcagt
    17701 ccatattact acgaataata aataaggaat aaacttcatt ttctcagcga ttgaaccaga
    17761 taaaatagaa attgcaatcg tacaaaacat catttgaaat aacataaaca aagcgaaagg
    17821 aatatgtggg ctaatatctt cttgagtcgc aaaacctaca tgattaagaa aagtatattc
    17881 ccaatttccg aaccataaat tcccttcccc aaaactaatt gtaaaaccaa ctgttatcca
    17941 tacaaatgta acaagcacaa ttgctgccat actttgcatg acagtattaa gcgcattttt
    18001 agattgaact aacccaccat aaaataaact taatcctggt gtcattagcc aaactaataa
    18061 tgtacacaaa aacataaata tcgtatcgtt aagattcata cttaccactc ccttgttttc
    18121 ataataatct gacaatttat aaaaagcaaa aatgcgttaa gttttattac atcttttgga
    18181 ataaaatgtg ccacactata taaaaatgat ttcatgaact aaaacaaaaa agactactta
    18241 ctcactttta aattagcgga agtagccata gaatctaaca tctattttat tgttaaaatg
    18301 ataacgttaa actttttgaa ttgtaattgt ccatgaagca ttatcaattt gttcatagtt
    18361 tgttacagga tatccatttt ctgctgccca atttggaata gcttccgtcg cttgcgtgca
    18421 atcaaaatca atttttaatt catctccaga ttgcaatgtt gccattttct tttgcgcttc
    18481 aattaacgga aatggacata ccattcctac tgtacctaat tcgtgtatca taattatttc
    18541 tccttttcca atcactttta tttgatcaac atcatttgca taataatctt ctatactatt
    18601 tgcgtttgct gtttaacctt tgcagattgt tgttgtactt ttttcattgg acgaacaaag
    18661 ataaaatgac tcattgtcca tacaccaact atcattgatg ctagcgcaat ccatccttgc
    18721 caagtcatcg ttgctgtttc aaccaaaccg ttaccgatag aacaaccacc agcaactgac
    18781 gcaccaaatc ccatacatat gccaccaata gcactgtttc gtattgtaat cttatctggc
    18841 aatcgccatt taaattctct tgatcctcta gctgcaatat atgaaccaat gaaaattcct
    18901 agaactaaaa agacacccca atcaataaat ttagtttcac ctgtaatcaa aaagtgtact
    18961 aaatttgctg atggcgttgt tatacctaaa ccgtcatttc ttccagttga tgcactcatt
    19021 ggccaagcta agagtgcgat aagtccaatt acaattgctg caataaatgg atggtatcgt
    19081 ttttcgaaaa ggtaatatct aatacctgta taacgttgct ttagttttgg aacagcaact
    19141 cgaactttct tattattgag tgttcttaca actagaaaaa tggtgattat agttaatata
    19201 acgactaatc cccaaaacgg aatgccagtt gtttgagaca tatcactatt tacattcgtt
    19261 ggttgattaa ttttatccat tactggcttt aaaatccctg ttttagtgat tgctgcagta
    19321 acagcatata atactaatgc aatccaacta ccaattagcc cttcaccagc gcgataccaa
    19381 gtacctgttg cacaccctcc agccaatact attccaattc caaaaataaa agaacctata
    19441 attgttccca atattggaaa actatgtgca ggaatttgta aaatatctgt tgccgtcaaa
    19501 atcaataatc ctatactttg aatagtaata gcgattaata atgcatagaa catcttatta
    19561 ttcttttgca catacatatc tcgaaagcca cctgttaaac aaaatcttgt tctttgcatg
    19621 acaaaaccta agagtccccc gacaattaag ccactaaaaa tcatccacat aatcctaacc
    19681 tctcatttcc gataagttta gtatgtatta taggtgatga ttttcttgtt ttcaacctta
    19741 tacacaatat ttttgatatt tatattatat tcatataagg aaggcgatta attacgattc
    19801 atcattatat ctctacgtgt taatttatta acctatacta tatttatcaa cacaacagtt
    19861 tttcctttca ttttcaaaca taacagaaaa agcctgagac atacttagtc ccagactgtt
    19921 ctataattaa ttaaagatat aacaaggtta atcattcaaa gctaacatca catttattca
    19981 ctgtaatttt tcataaagaa taataacgac tgtaattcta tacctaagtc aatttgatgt
    20041 acttgcacat ctgaaggcgt attaattcta ccaggagtga agtttaaaat acctttcaca
    20101 ccagcttgga cgagttcatc tgcaactttc tgtgcaactc tttctggtgt agttagaatc
    20161 acaacatcta tttcttcctt cttcaatgtt gttattaatt catcgttatc ttttacaata
    20221 acgttcccta ttttctggcc aataacatct tcttttacgt caaacgcttc tgtaatcgtc
    20281 atatcgtcat gtattgaaaa gttatatgtg agcaaagctt tccctaggtt cccaactccg
    20341 acaattgcga ttttgatcat gtcactctcg cttagttcag atttaaagaa atccaataaa
    20401 ctatctatat tatatccgta ccctttttta cctaattcgc caaaatatga aaagtcacga
    20461 cgaattgttg ccgagtcaat ttgtaacgca tcgctaatcg cttttgaatt tacacgatct
    20521 atacctttag attttaatga actgacaaat ctataatata acggcaaacg ttttaaagtt
    20581 gctcgaggaa ttttaacttg gtcactcatt cgctatttcc tccttcgtgt ttgaatgaat
    20641 tatatctatg tattcaaacg agttagagcg taattgttag attattaaaa aacaaataat
    20701 tgattacaat
//
