LOCUS       NZ_CP102415            22023 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Amycolatopsis roodepoortensis strain ZEL-1 chromosome, complete
            genome.
ACCESSION   NZ_CP102415
VERSION     NZ_CP102415.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis roodepoortensis
  ORGANISM  Amycolatopsis roodepoortensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Biodegradation of petroleum hydrocarbons by Amycolatopsis orientalis
            ZEL-1, genome analysis and its potential in plant protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) College of Life Sciences, Sichuan Normal
            University, No. 1819, Section 2, Chenglong Avenue, Longquan
            District, Chengdu 610101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024628825.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 06:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,064
            CDSs (total)                      :: 8,994
            Genes (coding)                    :: 8,928
            CDSs (with protein)               :: 8,928
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 44 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6777061
            Orig. end                         :: 6799084
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22023
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="24"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22023
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22023
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12023](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12023)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    485..496
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.32"
                     /tool="antismash"
     misc_feature    973..988
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.28"
                     /tool="antismash"
     gene            1077..1553
                     /locus_tag="NQK81_RS32445"
                     /old_locus_tag="NQK81_32445"
     CDS             1077..1553
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033810.1"
                     /locus_tag="NQK81_RS32445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32445"
                     /product="DUF4262 domain-containing protein"
                     /protein_id="WP_126735678.1"
                     /transl_table=11
                     /translation="MCWHCDNPGKTRADYLVEEVRPLIRKYGWMVQTVERGTVQPGFAY
                     TVGLTDAGLPELVVTGLRERRSGQLLNYFAQQVVRSGPPEAGEVLPAAVGWPSFEVVSL
                     SSPSAHLLTAVLLYGEEFRALQLVYEDEHGNWPWDRDFRGGTGGQPVLGVRGRG"
     gene            complement(1558..2250)
                     /locus_tag="NQK81_RS32450"
                     /old_locus_tag="NQK81_32450"
     CDS             complement(1558..2250)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033809.1"
                     /locus_tag="NQK81_RS32450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32450"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_192743318.1"
                     /transl_table=11
                     /translation="MSLPDIEPVSRESTAGIIARQLRDAIMTGALPPGTQLGETDLASR
                     FQVSRGPLREAMQHLVSEGLLRSERHRGLFVIDLEPGDVYDIYSARSAIERAAMLRALR
                     GDRERVAAELEEAVRAMATAADDDDPTALSWADLRFHEALIAASGSKRLVRMARTLLIE
                     TRMCLTALQGTYQRVEERVTEHTRIIEALRAGDEETALSLLEAHMEDAVQRLAPGTSLR
                     EGEAPAVP"
     gene            complement(2254..3024)
                     /locus_tag="NQK81_RS32455"
                     /old_locus_tag="NQK81_32455"
     CDS             complement(2254..3024)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033808.1"
                     /locus_tag="NQK81_RS32455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32455"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_257484505.1"
                     /transl_table=11
                     /translation="MPITPFAPPEPPSETTTIGFIYPDHAAEDDYPLAEQLLGGDMAGI
                     KLPVEHIYGTDLHAVPELLDLGSESRLADGAALLAKHEPDAVVWACTSGSFVYGWEGAH
                     DQADRLSAVAGVPASSTSFAFVNAARALGVRRVAVAASYPDDVARLFVEFLGAGGVEVV
                     SMGSADIDTAAEVGELSPEAVVELAVSRDHPDADALLVPDTAMRTLGEINTLETRLGKP
                     VLTANQVTVWEGLRLTGKSPLVRTLGALFGRRGD"
     gene            complement(3069..3809)
                     /locus_tag="NQK81_RS32460"
                     /old_locus_tag="NQK81_32460"
     CDS             complement(3069..3809)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631263.1"
                     /locus_tag="NQK81_RS32460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32460"
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="WP_126735675.1"
                     /transl_table=11
                     /translation="MDFDLLEFEGPLAQRGIGVIAPFDLALERELWRWVPMEVSLHLAR
                     TPYEPVPVSMEMAQLVSDSRHLAAATRDVLHVEPEVVAYLCTSGSFVNGVDYERSLTKA
                     ICDAGATDAVTTSGALAEVLHQLDLHRVSVLTPYDGDLTGKLHDFLAELGVDTVSSDHL
                     GLGGGIWKVSYRTIAERILAADHADAEAIFVSCTNLPTYDLIEPLESALGKPVLTANQL
                     TMWACLRRMNLPIVGPGKWLREVD"
     misc_feature    complement(3795..3797)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3908..4897
                     /locus_tag="NQK81_RS32465"
                     /old_locus_tag="NQK81_32465"
     CDS             3908..4897
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336991.1"
                     /locus_tag="NQK81_RS32465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32465"
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="WP_257484506.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.5e-51, bitscore:
                     173.3, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIASETPVLAVLCGDTRPPDMRAIEDRAVVRYTGEAGLADALSGA
                     DAFFVYDFLSTAVPGAWHAADKLRWLHIASAGVDPVLFPGLQESDVVLTNSRGVFDGAI
                     AEYVLGVVLAFAKDFVRSWDLQRRKQWKHRESERISGRRVLVVGTGPIGRSIARLLRAA
                     GMSVAGVGRRPREDDPDFGDVYASSDLARHLPGADYVVAVAPLTEQTKGMFDAETFAAM
                     KPGARFVNVGRGELVVTSDLIGALRDGPLGGAALDVFDTEPLPADSPLWTMENVLISPH
                     MSGDFVGWRNTLVEVFADNFRRWRAGEPLRNVVDKQLGYVPSETLRQG"
     misc_feature    4031..4033
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4905..6281
                     /locus_tag="NQK81_RS32470"
                     /old_locus_tag="NQK81_32470"
     CDS             4905..6281
                     /EC_number="3.5.1.4"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150551.1"
                     /locus_tag="NQK81_RS32470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32470"
                     /product="amidase"
                     /protein_id="WP_126735673.1"
                     /transl_table=11
                     /translation="MNDTKLTASELVAAYATGELSPVEATQNALRAIEERDGETNAFCL
                     VDADGALEQAKASEIRWRDGNPIGWLDGVPASIKDMFLTQGWPTLRGSRCIDPDQPWDV
                     DSPVTARLRENGLVLLGKTTTPELAWKGVTDNTLTGITRNPIDPSTTAGGSSGGSAAAV
                     AAGMGELSVGTDGGGSIRIPASFCGIVGLKPTHGRIPLFPASPFGPLSHAGPMARSVDD
                     TALLLDVLALPDHRDPAALAPPVSTYREAVRRDVRGLIAAYSPTLGYVDVDPEVAEIVK
                     SAVRSLGDAGLHIEETDPGFTDPKAAFDILWSTGAAKWLDTFPAGSETKVDPGLRKVWE
                     LGKTFSASDYLGANAERAALGILMGEFHTRYDVLITPTLPITAFEAGHEVPPGSGLSGW
                     PDWTPFTYPFNMTQQPAISVPAGRTSRGLPVGLQIVGPRHSDDLVLAVAKLLEEVRPWP
                     "
     gene            complement(6333..7553)
                     /locus_tag="NQK81_RS32475"
                     /old_locus_tag="NQK81_32475"
     CDS             complement(6333..7553)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631260.1"
                     /locus_tag="NQK81_RS32475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32475"
                     /product="MFS transporter"
                     /protein_id="WP_257484507.1"
                     /transl_table=11
                     /translation="MTSLREPLRHHNFRWLIGGRTFGEFGNAVAPLALAFAVIDLTGSA
                     VDIGIVVGARSVASVVLLLFGGVLADRLPRSVILQGTELAATLTQAVIAASVLCGFASI
                     PLLVGLSVVNGAVAAISAPASASLTPLTVPATLLAQANALARLLSNSGRIAGAGLGGIL
                     VTAVGPGWALGGNALLFLGSALSYRCIRLSRRERIPGSRPLAELVEGWHEFRSRTWVWV
                     VVVQFMVVNAVIAGGIAVLGPVVADSTFGRSGWGFALAAQTIGSLVGGILVARWQPRRA
                     LFVGVAVILFEAPPLILLGQAPLLPLLLAAMFVSGLAIEQFVVAWDVSLQENVPEDKLA
                     RVYSYDMLGSFIALPLGQMAAGPAAQHFGVSTTLLACAALVVAATGLALCSAQVRGLVR
                     KSHAAQP"
     gene            complement(7550..8068)
                     /locus_tag="NQK81_RS32480"
                     /old_locus_tag="NQK81_32480"
     CDS             complement(7550..8068)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631259.1"
                     /locus_tag="NQK81_RS32480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32480"
                     /product="transcriptional regulator"
                     /protein_id="WP_257484508.1"
                     /transl_table=11
                     /translation="MPESPGLDELRVLAHPVRLRILSLLTGVAMSAAEAARELGETQAN
                     VSYHLRRLHEAGLLDVAEEVRIRGGLAKRYRHDPESGSRFTSANPREEQLLIATMAEEL
                     KRRSEFRVPGKPGSTSDAEMWVDRETWGKALEHARELSDLLHSAAKPPRTRGTIPVSAT
                     VALFEMRRR"
     misc_feature    8102..8115
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.81"
                     /tool="antismash"
     misc_feature    8150..8165
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.59"
                     /tool="antismash"
     gene            8176..9150
                     /locus_tag="NQK81_RS32485"
                     /old_locus_tag="NQK81_32485"
     CDS             8176..9150
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082804.1"
                     /locus_tag="NQK81_RS32485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32485"
                     /product="aldo/keto reductase"
                     /protein_id="WP_257484509.1"
                     /transl_table=11
                     /translation="MRTTTLGRSGLEVSRIAFGTWQLGGDWGSFDEDAAIAAIHHAREL
                     GVNFFDTAQAYGFGKSEAMLGKALREELKRDRDNLVIATKGGIKPRSERPRDSRREWLT
                     QGIEDSLRFLDVDHIDLYQIHWPDPGTPAEETAGILQEFVDAGKIRHVGVSNYDAAALA
                     AFDKTRPVETLQPPYHLFRRDIETDPLPYARENDIGVLVYSPLGSGLLTGSFTPETTFE
                     KTDWRSRSSAFTGETFRRNLAVVDRLEEFAADKGISVSQLAIAWTLAQPGVHVAIVGAR
                     KATNIEASLAAAGIDLTADDLAEIDRLTADAVPVSGASPEGVA"
     gene            complement(9147..10004)
                     /gene="pqqB"
                     /locus_tag="NQK81_RS32490"
                     /old_locus_tag="NQK81_32490"
     CDS             complement(9147..10004)
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631257.1"
                     /locus_tag="NQK81_RS32490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32490"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_257484510.1"
                     /transl_table=11
                     /translation="MRVILLGTAAGGGFPQWNCACALCVSGAPPRTQDCVAVSADGEGW
                     YLLNVSPDIRAQILATPPLRAGPGPREIPLRGVLLTDAELDHSLGLLLLREAGGLPVWA
                     PDAVLGALSEQFPARSIIDGYGGWDWLPSSEVSIDGLRVSVLPVSDKRPKYARSSTEDG
                     PWVVAYRIEDVETGGVFVYAPCLKSWPDGFDDFTRDAGLVLLDGTFYAPEEMSGATAAE
                     AGAQLAMGHLPITGSLAKLRPGPRWVYTHLNNTNPVVDPASPEHAAVLDGGASLPLDGT
                     EFKL"
     gene            complement(10001..11080)
                     /gene="pqqE"
                     /locus_tag="NQK81_RS32495"
                     /old_locus_tag="NQK81_32495"
     CDS             complement(10001..11080)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150538.1"
                     /locus_tag="NQK81_RS32495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32495"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_257484511.1"
                     /sec_met_domain="PF04055 (E-value: 2.5e-22, bitscore: 79.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.4e-05, bitscore: 24.4,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.1e-09, bitscore: 36.7,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 9.3e-10, bitscore:
                     37.8, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLNTAAPLGMLAELTHRCPLHCPYCSNPVELITRDSELSTEEWL
                     SVLTQARELGVLQVHMSGGEPLARPDLPILVEHATKLGCYVNLVTSGLGLTESRLDDLA
                     ERGIAHIQLSVQGATADRADRLAGARAHDRKLIAAGLIKKAGLPLSVNVVLHRQNHDQL
                     AGIIDLAEEMGADRLELANTQYYGWALRNRDALMPTRRQLDEAEPIVRAATERLRGRME
                     IIYVVADYYEKYPKPCMYGWGARQLTVAPDGNVLPCPASTAIETLEFDNVRDKPLAWIW
                     YESGSFNAFRGEDWMPDTCGTCDRRGIDFGGCRCQAFQLTGDASATDPVCSRSPQRGVV
                     DLILATQAKTDELVMRTPR"
     gene            complement(11071..11343)
                     /gene="pqqD"
                     /locus_tag="NQK81_RS32500"
                     /old_locus_tag="NQK81_32500"
     CDS             complement(11071..11343)
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336984.1"
                     /locus_tag="NQK81_RS32500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32500"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_126735668.1"
                     /sec_met_domain="PqqD_RRE (E-value: 6.1e-23, bitscore:
                     79.4, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7.1e-10, bitscore: 38.1,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLEPGSVPRLRRGVRLSYDQTRETHVLLFPEGVLVPNPTAAAV
                     LSLCDGVEDVAGIASALRERYTGVREEEILDVLSRLGERRIVEWT"
     aSDomain        complement(11080..11340)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_NQK81_RS32500_PqqD_RRE.1"
                     /evalue="1.90E-25"
                     /identifier="RREFam006.1"
                     /label="NQK81_RS32500"
                     /locus_tag="NQK81_RS32500"
                     /protein_end="88"
                     /protein_start="1"
                     /score="79.4"
                     /tool="antismash"
                     /translation="TTLEPGSVPRLRRGVRLSYDQTRETHVLLFPEGVLVPNPTAAAVL
                     SLCDGVEDVAGIASALRERYTGVREEEILDVLSRLGERRIVE"
     gene            complement(11346..12023)
                     /gene="pqqC"
                     /locus_tag="NQK81_RS32505"
                     /old_locus_tag="NQK81_32505"
     CDS             complement(11346..12023)
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658743.1"
                     /locus_tag="NQK81_RS32505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32505"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_126735667.1"
                     /sec_met_domain="TIGR02111 (E-value: 2.4e-85, bitscore:
                     284.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSSEFIAALRGLSDRYWGTHPFHVRMHEGHLSARELKIWAANRW
                     YYQCVLPQKDAAIISNCPLPEIRREWLDRIVYHDGTKAGDGGIERWLRLCTAVGLDREE
                     VLDQRHVAPGVRFAVDAYVNFARTKPWLEAVASGLTEMFAGHLMQRRVADMLANYSWIA
                     PDDLAYFTNRIDKVSGEGKGTLDIVVRHAVTREQQDKAIAALSFKTDVLWSMLDAIERA
                     AKE"
     gene            complement(12107..12214)
                     /gene="pqqA"
                     /locus_tag="NQK81_RS32510"
                     /old_locus_tag="NQK81_32510"
     CDS             complement(12107..12214)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033797.1"
                     /locus_tag="NQK81_RS32510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32510"
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_126735666.1"
                     /transl_table=11
                     /translation="MWTLGAEMIEEWTTPDFVEYETPMEVTAYAARMED"
     misc_feature    complement(12203..12205)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(12295..12308)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.78"
                     /tool="antismash"
     gene            12510..13496
                     /locus_tag="NQK81_RS32515"
                     /old_locus_tag="NQK81_32515"
     CDS             12510..13496
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631253.1"
                     /locus_tag="NQK81_RS32515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32515"
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="WP_257484512.1"
                     /transl_table=11
                     /translation="MSLRRTLAATFAAATAVSIAAVPAAAEAPPSTQAIRQLASGLNQA
                     WSIDFLPDGTGVFTQKDARTISKIDKAGKVTTVQTIPGVSVTAEAGLLGIAVSPNYATD
                     QTVFIYYTTSSDNRIAKLKLGQTPTPIVTGIPRGSQYHHGGRIRFGPDGYLYAGTGDGQ
                     NGANAQNKNSLGGKVLRVTTDGAAAPGNPFNSRVYSYGHRNVQGLTWANGQLYISDIGA
                     NKLDELNKIEPGKNYGWPTCEGPCNTAGMTNPVKSWPTSSATPSGLAAYKGSLYMASLK
                     GGTYKLDLNGNGGKLWTNLGRTRDATAGPDGQLYTITPGALYVSDGS"
     misc_feature    12516..12518
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(13622..14998)
                     /locus_tag="NQK81_RS32520"
                     /old_locus_tag="NQK81_32520"
     CDS             complement(13622..14998)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631252.1"
                     /locus_tag="NQK81_RS32520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32520"
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="WP_257484513.1"
                     /transl_table=11
                     /translation="MPFMPVTDSMFLLVETREHPMHVGGLQLFRKPEGAGDDYLRDVRK
                     SLLDSDNMRSVFRRRPARPVNTAGHVAWATDNDLELDYHFRHSALPQPGRIRELLELTG
                     RWHSTLLDRHRPLWEIHLVEGLQDGRFAIYSKIHHALMDGVSALRHLQGTLSDDPADLD
                     CPPPWGRRAKPDGGRNGKVSPSVFRTFGKTVNQLAGIAPAAMKVAREAFQEHTLTLPAQ
                     APKTMLNVPIGGARRFAAQSWSLDRVRKVATAAGVSRNDVVLAMCSGALRDYLMEQNAL
                     PDVPLTAMVPVSLRRKDSGDAAGNNIGALLCNLATHLTDPAARLATINASMRNGKKLFS
                     ELTPLQTLLLSGINVAQLGVSPIPGFVNNTKPPFNLVISNVPGPRKQMYWNGAALDGIY
                     PASVLLDGQALNITLTSNGDNLDFGITGCRRSVPHLQRILTHLDTALAELEHSVSIGRS
                     "
     gene            15094..16107
                     /locus_tag="NQK81_RS32525"
                     /old_locus_tag="NQK81_32525"
     CDS             15094..16107
                     /EC_number="1.8.5.-"
                     /GO_function="GO:0004364 - glutathione transferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033794.1"
                     /locus_tag="NQK81_RS32525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32525"
                     /product="glutathione S-transferase family protein"
                     /protein_id="WP_257484514.1"
                     /sec_met_domain="GST_C (E-value: 1.8e-07, bitscore: 30.3,
                     seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIPTDPQTGEFRRSANHFDARVTADGRDGWPVEAGRYRLVVSRA
                     CPWASRGLVVRRLLGLESALSVAVADPIQDEKSWRFTLDPGDRDPVLGIKYLAEAYHAA
                     DPHYMGGISVPAIVDIPSKRLVSNDYPQITLDLSTEWTSLHRPGAPDLYPERLRGEIDD
                     VNAGVYADVNAAVYQAGFATKQAAYEDAYTRLFTMLDVLSERLEKQRYLVGDTITEADI
                     RLFTTLVRFDAVYHGHFKCNRNKLTEMPVLWAYARDLFQTPGFGDTVDFDHIKRHYHAV
                     HEQVNPTRIVPLGPDLSGWTTPHHREQLGGRPFGDGTPPGPPPEDERIGDTDGATA"
     gene            16091..17248
                     /locus_tag="NQK81_RS32530"
                     /old_locus_tag="NQK81_32530"
     CDS             16091..17248
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150529.1"
                     /locus_tag="NQK81_RS32530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32530"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_257484515.1"
                     /sec_met_domain="DAO (E-value: 5.7e-69, bitscore: 232.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEPLPERTDVVVIGGGIIGVACAYRLAAAGVPVLLLERDILGAGS
                     TAKAAGGIRSSFTTRVNIEIGLRGLAEYASFAETYGTEIDFRRDGYLYLVTDPADLPEF
                     ERCAELQNSYGVRSRLVEPAEARRFSPLIDTEGVVAALWSPDDAKATPDSAVQGYARAA
                     RAHGAVLRTGVEVVGIERDGDVITGVRTSAGFVRTDAVVCAAGAWSGRIGELAGVDLPV
                     RPFRRQVVFTGPVADLPGSVPLTIEMPSAFYFHREGAGLAMSFCDGDGDPGFDTRYQPG
                     EWLPKLAEIAARRVPAVLDAGIRGGWAGLYEMTPDRNQIVGESVHLSRFFYATGFSGHG
                     FQMGPAVAELVRDLYLGRVSEIDISGLDVRRFAGDRAVTREHNIV"
     gene            17241..17864
                     /locus_tag="NQK81_RS32535"
                     /old_locus_tag="NQK81_32535"
     CDS             17241..17864
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336978.1"
                     /locus_tag="NQK81_RS32535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32535"
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="WP_257484516.1"
                     /transl_table=11
                     /translation="MSEFVEVTTEAELREILPPPLERTANKARPKLHEMDRQWLAESPF
                     VLIATSAADGTCDVSPKGDPAGFTLVLDDTRIAIPERPGNRRADGFRNVLSNPHVGLIY
                     LIPGRGDTLRINGRARLVREAPFFDDMIVNGNRPKLALVVDIDEIFHHCQKAFMRSKLW
                     SPESWAPDALPSRAVISKTFERPEDSLADLEAYYAPAAYSAGLY"
     gene            17974..19605
                     /locus_tag="NQK81_RS32540"
                     /old_locus_tag="NQK81_32540"
     CDS             17974..19605
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336977.1"
                     /locus_tag="NQK81_RS32540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32540"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_257484517.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1e-55, bitscore:
                     187.0, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4.8e-27, bitscore:
                     93.7, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAPTGAQHLADALTALGTEVVFGLPGVHNLPLWEALADTDIRLV
                     GVRHEQTAGYAADGYARATGKLGVALVTTGPGAANTLAAVGEAMASAAPVLIIATDIPS
                     TLRRPGVVRGVLHETSDQQAMFAPVTKAGFTVTSADQVAATLHRAARLALQPQSGPVYL
                     GIPADFLSERTPARRPPRSHARPAEIPDLTEAEALLATADRPLIWAGGGALRAEAGEAI
                     GKLAERLAAPVLTTFGARGLLPVDHPCLAPNPVHAPEVGALWDEADVVLAIGTDFDGLM
                     TQNWLMPKPKKLIAINVDPDDAAKNYQPDITLVGDARVLVEALKVEERPGIAALVGRLA
                     KVSARVRRRIKEEEPQAADLLSTLDEVLPPDAVVVADMCVAGYWIGGFHRVRAPRKLAY
                     PMGWGTLGYGFPAALGASAAGAGRAICVSGDGGFLFGCGDLHTLAQEQLPVTVILVDDG
                     GYGMLRYDQDRAGLPRRGVDWDSPDFVGLARAFGVHADRVSGFGRAFRRLLGEFVESDE
                     PNVLVVRAKMAPPLNTSPRWYRKESP"
     misc_feature    18070..18072
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            19602..21662
                     /locus_tag="NQK81_RS32545"
                     /old_locus_tag="NQK81_32545"
     CDS             19602..21662
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150523.1"
                     /locus_tag="NQK81_RS32545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_32545"
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="WP_257484518.1"
                     /transl_table=11
                     /translation="MTGLRVGVDIGGTFTDLCVLDGTGLVAVGKVLTTHDEPARAVEEG
                     LKRTLADAGLDAGDVEQFVHGTTLVTNALIERKGARTALLATAGFRDVLEMRREHRYEL
                     YDLLIELPAPLVPRHLRFDIPERILADGTVHTGLDEEYVARLGRELDARGIDAVAICFL
                     HAFTNPAHERRAAEVLREVAPRLRVALSSEVVPEIREFERMSTTVGCVYVQDLTERYLR
                     DLERRLHEAGVRPSPHIMLSNGGIATVDTAARYPIRILESGPAGGALAASAIGAAAGVE
                     DLLAFDMGGTTAKLCLISDGAPLVTHEFEVDRKYRLLPGSGLPVKVPVTDMIEIGVGGG
                     SIARIDALGLLTVGPDSAGSEPGPVCYGRGGAEPTVTDADLVLGYLDPGYFLGGGMTLD
                     LEGAREAIRSKIAGPLGVSVEEAAWGIHTSVNEDMANAARVHAVERGKDPAKLPMFTFG
                     GAGPVHGVGVARALGAPEVVAPPAAGVLSAAGFLTAPLAFDFVRSARSAVLDISWEQVD
                     ARFAEMEAEGAELLEKSGVDGSDVTHRRIAEMRYSGQGYEIRVPVGRGSWPESLIDAFT
                     ETYRTLYRRAGPDVAIEVLNWRVVSSGPTPEVTLKLAGAEAEGDARKGSRPAYFPGEGG
                     FVDTAVFDRYRLKPGDRVEGPAIVEERESTVVVPPGARCVVGADASLVVTV"
     misc_feature    21808..21822
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 cacgagggtc ggggtcgagc ggatcatccg ctacgccttc gaactggcga agacgcgcac
       61 ggggcgggtc tgttcggcga ccaaatccaa cgggctcatc cactcgatgc cgttctggga
      121 cgagatcttc gcggaaatct ccgcggagta cccggacgtg cacgccgagc agtgccatgt
      181 cgacgcgctc gccgcgcgga tggtgcaggc gccggaccgg ctcgacgtcg tggtggcttc
      241 caatctgttc ggcgacatcc tgagcgacct ggcggcggcc gtgacgggcg ggctcggaat
      301 ggcaccttcg ggcaacatca atccgaccgg tgagcttcct tccatgttcg aggcggtgca
      361 cggcagcgct cccgacatcg ccggacaggg catcgcgaat ccggtggcgc agatcctggc
      421 gggggcgatg ctgctcgacc atctcgggga aaccgttgcg gcacaagccg tacgtgcggc
      481 ggtggaaaag gttctcgagg agggggccgt gcaaaccccc gacctcggcg ggaagtccac
      541 caccgccgaa ctcgggtccg cggtggtcgc cgctctatct tgaaccatcc gcatagtggc
      601 gtgtcaagtc ccggaagtca tgaacggtcc attcatggct gttgagtaca ggaaggcccc
      661 ttctttgcgt taagcgcagg gaaggggcct tcgcgtactc aagttcgggc ggagccgcca
      721 ttcgctgact ggagcggcaa ggctcgacgg ccgtgtcgcg aaagccactt tcgggacatc
      781 aggtgtcctg aaagtggctt tcgcgacact ggacaggtcc aggaagtgcc agattcacct
      841 tgcgacgtta ggcagtggcg tttgggtgtc gatcccgtgg atccatcgcg caacctgcgt
      901 cttgaccttc gaaatttctc aaccaaggtg aaaatgttgc cctgaaaggg gaatctcggc
      961 gcgaaatcga gcacgaaagc ccgttcgccg actgcgcgtc accccgggtc gcgagttttt
     1021 gtcggtgccc cggtgcacag tgccggtcat cggattcgac gacttgagga gcgctgatgt
     1081 gctggcattg cgacaacccc gggaagaccc gcgccgacta cctcgtcgag gaggtgcggc
     1141 cgctgatccg gaagtacggc tggatggtgc agacggtcga gcgcggcacg gtccaaccgg
     1201 gcttcgcgta cacggtcggg ctcaccgacg ccgggctgcc ggaactggtc gtcaccggcc
     1261 tgcgggagcg aagatccggc cagctgctga attatttcgc ccagcaggtg gtccggtcgg
     1321 ggccgccgga ggcgggtgag gtgctgcccg ccgcggtggg atggccgtcg ttcgaggtcg
     1381 tttcgctcag ttcgccgtcg gcccacctgc tcaccgcggt tctcctgtac ggcgaagaat
     1441 tccgtgcgct gcaactggtc tacgaggacg agcacggcaa ttggccgtgg gacagggatt
     1501 tccgcggcgg gacgggtggt cagccggtgc tgggggtgcg aggccgtggc tgaaggctca
     1561 aggcaccgcg ggcgcttcgc cttcccgcag gctggttccc ggggccagcc gctggacggc
     1621 gtcctccata tgggcttcca gcagcgacag cgcggtctcc tcgtcgccgg cgcgcagggc
     1681 ttcgatgatc ctggtgtgct cggtgacccg ttcttcgacc cgctgatagg tgccctgcag
     1741 cgcggtgagg cacatacggg tctcgatcag cagggtcctg gccatgcgca cgagccgctt
     1801 gctgccggag gcggcgatca gcgcctcgtg gaaacggagg tccgcccagg agagcgcggt
     1861 cgggtcgtcg tcgtccgcgg cggtggccat ggcacgcacg gcctcttcga gttcggcggc
     1921 gacccgctcc ctgtccccgc gcagcgcgcg cagcatcgcg gcacgctcga tggccgagcg
     1981 cgccgagtag atgtcgtaga cgtcgccggg ttcgaggtcg atcacgaaca ggccgcggtg
     2041 gcgttcgctg cgcaggaggc cttcggagac gaggtgctgc atcgcctcgc gcagcggccc
     2101 ccgcgaaacc tggaaccggg aggccagatc cgtttcaccg agctgggtgc cgggaggaag
     2161 agcgccggtc atgatcgcgt cccgcaactg gcgcgcgatg atcccggcgg tcgactcccg
     2221 gctgaccggc tcgatatccg gcaaggacat ggttcagtcg ccccttctcc cgaacagcgc
     2281 gcccagtgtg cggacgagcg gcgacttccc ggtgagccgc aacccttccc agacggtgac
     2341 ctggttggcg gtcagcacgg gcttgccgag cctggtttcg agcgtgttga tctcgccgag
     2401 cgtccgcatg gcggtgtcgg ggacgagcag cgcgtcggcg tccgggtggt cccggctcac
     2461 cgcgagctcg acgaccgctt ccgggctgag ttcgccgacc tcggccgcgg tgtcgatgtc
     2521 cgcgctgccc atcgagacca cttcgacccc gcccgcgccg aggaactcga cgaacagccg
     2581 ggcgacgtcg tccgggtagc tggcggcgac cgcgacccgc cggacgccga gtgcccgcgc
     2641 ggcgttgacg aaggcgaagg aggtgctcga cgccgggaca cctgcgacgg cggagagccg
     2701 gtcggcctgg tcgtgggcgc cttcccagcc gtagacgaag ctgccgctgg tgcacgccca
     2761 caccaccgcg tcgggttcgt gtttggccag gagcgcggcg ccgtcggcga gccggctttc
     2821 gctgccgagg tccaagagct cgggaacggc gtgcagatcg gttccgtaga tgtgctcgac
     2881 cggcagcttg atcccggcca tgtccccgcc gaggagctgt tccgcgagcg ggtagtcgtc
     2941 ttcggccgcg tgatcggggt agatgaaccc gatggtggtc gtctcggacg gcggctcggg
     3001 tggggcgaac ggcgtgatgg gcacggaacc tccgaagggc agattgtcga caatcgtagg
     3061 cgaagaggtc agtcgacctc ccggagccac ttgccgggcc cgacgatcgg caggttcatc
     3121 cgccggaggc aggcccacat ggtgagctga ttcgcggtga ggaccggttt gccgagcgcg
     3181 ctttcgagtg gctcgatcag atcgtaggtg gggaggttgg tgcagctgac gaagatggct
     3241 tcggcgtcgg cgtggtcggc cgcgaggatg cgttcggcga tggtccggta gctgaccttc
     3301 cagatgccgc cacccaggcc gagatggtcg ctcgagacgg tgtcgacgcc gagctcggcc
     3361 aggaagtcgt gcagcttccc ggtcaggtcg ccgtcgtagg gggtgagcac cgagacccgg
     3421 tggaggtcga gctgatgcag gacctccgcc agcgctccgg acgtggtgac ggcgtccgtc
     3481 gcgcccgcgt cgcagatcgc cttggtcagc gagcgttcgt agtccacacc gttgacgaaa
     3541 ctgccggagg tgcacagata agcgacgact tcgggctcca catggagcac gtcccgcgtg
     3601 gcggcggcga gatgcctgct gtcgctgacg agctgggcca tctccatgct gacgggcacg
     3661 ggctcgtacg gtgtccgcgc caggtggagg gagacctcca tgggcaccca gcgccacagc
     3721 tcgcgttcca gggccaggtc gaagggggcg atcacgccga taccgcgctg cgccaacggg
     3781 ccttcgaact ccagtaagtc gaaatccaag tctcagcctc cggaaacttc tgctgtcaca
     3841 gtccggtgct ccgggggtcg ttcctcggat tgttgacaat catacgagcc acttttacgg
     3901 tgtcaacgtg atcgcctcgg agacccctgt gctggcggtg ctctgcggag acacgcgccc
     3961 accggacatg cgcgctatcg aagaccgagc ggtggttcgt tacaccggtg aggccgggtt
     4021 ggctgacgcg ttatccggag ccgacgcgtt cttcgtctac gacttccttt cgaccgccgt
     4081 cccgggcgcc tggcacgcgg ccgacaagct gcgctggctg cacatcgcca gtgcgggcgt
     4141 cgaccccgtc ctcttcccgg ggctgcagga gagcgatgtc gtgctcacca actcccgagg
     4201 ggtcttcgac ggggcgatcg ccgagtacgt cctcggagtc gtcctcgcgt tcgccaagga
     4261 cttcgttcgt tcgtgggatc tgcagcggcg aaagcagtgg aagcaccgcg agagcgagcg
     4321 gatctccggc cgccgggttc tcgtggtcgg cacgggtccg atcggccggt cgatcgcgcg
     4381 gctgctgcgg gcggccggga tgtcggtggc gggcgtcgga cggcgtcctc gtgaggacga
     4441 tcccgacttc ggcgatgtgt acgcgtcttc ggacctcgca cgtcatcttc ccggggcgga
     4501 ctacgtcgtc gcggtcgcgc cgttgaccga gcagacgaag ggcatgttcg acgccgagac
     4561 cttcgccgcg atgaaacccg gcgcgcggtt cgtcaacgtc ggccgaggtg aactggtcgt
     4621 tacctcggac ctgatcggcg cactgcggga cgggccgctc ggcggcgcgg cgctcgacgt
     4681 cttcgacacc gagccgctgc ccgccgacag tcctttgtgg actatggaga acgtgctgat
     4741 ctcgccgcat atgtcgggcg acttcgtcgg ctggcgtaat actctggtag aggtgttcgc
     4801 ggacaacttc cgtcgctggc gcgcggggga gccgctgcgc aacgtcgtgg acaagcagct
     4861 cgggtacgtg ccgtcggaga cgcttcgcca aggttagggg ccggatgaac gacaccaagt
     4921 tgaccgcgag cgagctcgtc gccgcgtatg ccaccggaga gctgtcgccg gtcgaggcca
     4981 cccagaacgc cttgcgcgcc atcgaagaac gcgacggcga gaccaacgcc ttttgccttg
     5041 tcgacgccga cggcgcgctc gaacaggcga aagcgtccga gatccgctgg cgggacggca
     5101 acccgatcgg ctggctcgac ggcgttccgg cctcgatcaa ggacatgttc ctgacccagg
     5161 gctggccgac gttgcgcggt tcgcgatgca tcgaccccga ccagccgtgg gacgtcgaca
     5221 gcccggtcac cgcgcggctg cgtgagaacg gcttggtgct gctgggaaag accacgacac
     5281 cggagctggc gtggaagggc gtcaccgaca acacgctgac cgggatcacc cgcaacccga
     5341 tcgacccgtc gacgacggcg ggcggttcga gcgggggcag cgccgcggcc gtcgcggcgg
     5401 ggatgggtga actctccgtc ggcaccgatg gcggcggctc gatccggatc ccggcgtcgt
     5461 tctgcggaat cgtcgggctc aaaccgacac acggccggat cccgctgttc ccggcgagtc
     5521 cgttcggccc gctctcgcac gccggcccga tggcgcgttc ggtcgatgac accgcgctgc
     5581 tcctcgacgt cctcgccctg ccggaccacc gtgacccggc cgcgctcgcc ccgccggtct
     5641 cgacctatcg cgaggccgtc cggcgggacg tgcgcgggct gatcgccgcc tactcgccga
     5701 cgctcggcta cgtcgacgtc gacccggagg tcgcggagat cgtcaagtcg gcggtccggt
     5761 cgctgggcga cgcgggcctg cacatcgaag agaccgatcc cggtttcacc gacccgaaag
     5821 cggccttcga catcctgtgg tcgacggggg cggccaagtg gctggacacc ttccccgccg
     5881 gttcggagac gaaggtcgat cccgggctgc ggaaggtgtg ggaactcggc aagaccttct
     5941 cggcgagcga ctacctcggc gccaatgccg agcgggccgc gctaggcatc ctgatgggcg
     6001 agttccacac gcgctacgac gtgctgatca cgccgaccct cccgatcacc gccttcgagg
     6061 ccgggcacga ggtgccgccg ggcagcgggc tgagcggctg gccggactgg acgccgttca
     6121 cctacccgtt caacatgacc cagcagcccg ccatcagcgt gccggcgggc cgcacctcgc
     6181 ggggcctccc ggtcggcctg cagatcgtcg gaccgcggca ctccgacgac ctggtcctcg
     6241 cggtcgcgaa gctcctcgag gaggtccgtc cctggccgtg acctgcggaa aggaagcaag
     6301 ggacctttgc taccacttcg cggggcgccg ggtcagggct gcgcggcgtg ggacttccgg
     6361 accaggccgc gcacctgggc gctgcacagc gcgagaccgg tggcggccac gaccagcgcg
     6421 gcgcacgcga gcaaagtggt gctgaccccg aagtgctgcg cggcgggccc ggccgccatc
     6481 tgcccgaggg ggagggcgat gaaggaaccg agcatgtcgt aggagtagac gcgcgcgagt
     6541 ttgtcctcgg gcacgttctc ctgcagcgag acgtcccacg cgaccacgaa ctgctcgatc
     6601 gccagaccgg agacgaacat ggccgccagc agcagcggca gcaagggggc ctggccgagg
     6661 aggatcagcg gcggcgcctc gaagaggatc accgcgacgc cgacgaacag cgcgcgccgc
     6721 ggctgccatc gggccacgag gatgccgccg acgagcgagc cgatcgtctg cgcggcgagg
     6781 gcgaatcccc agccggagcg cccgaaagtg ctgtcggcga cgacggggcc gagcaccgcg
     6841 atcccgccgg cgatgacggc gttcaccacc atgaactgca cgaccacgac ccagacccag
     6901 gtgcgcgacc ggaactcgtg ccagccttca acgagttccg cgagcgggcg gctgccgggg
     6961 atccgttccc ggcggctgag gcggatgcac cggtaggaca gggcggagcc caggaacagc
     7021 agcgcgttcc cgccgagtgc ccagccgggg ccgaccgcgg tgaccaggat gccgccgagc
     7081 ccggcgccgg cgatccggcc ggagttcgac aacagcctgg ccagcgcgtt ggcctgtgcg
     7141 agcagggtgg cgggcaccgt cagcggggtg agtgacgccg acgccggtgc ggagatcgcg
     7201 gccaccgcgc cgttgaccac gctcaggccg accagcagcg ggatcgacgc gaatccacaa
     7261 aggacgctcg ccgcgatgac cgcctgggtc agcgtggcgg cgagttcggt gccctggagg
     7321 atcaccgacc gcggcagccg gtccgccagt accccgccga acagcagcag gaccacgctc
     7381 gccacggacc gcgcgccgac gacgatgccg atgtcgacgg ccgaaccggt caggtcgatc
     7441 accgcgaacg cgagcgcgag cggggcgaca gcgttgccga actccccgaa cgtgcggccg
     7501 ccgatcagcc agcggaagtt gtgatggcgc agtggttcgc gcagagacgt catcgtcgcc
     7561 tcatttcgaa cagcgcgacc gtcgcgctga ccgggatcgt gccccgggtg cggggcggct
     7621 tcgcggcgga gtgcagaagg tcgctcagct cgcgggcgtg ttcgagcgcc ttcccccagg
     7681 tctcgcggtc gacccacatc tccgcgtcgc tcgtcgaccc cggtttgccg ggaacgcgga
     7741 attcgctgcg ccgcttgagt tcttcggcca tagtcgcgat gaggagctgt tcctcccgcg
     7801 gattcgccga cgtgaaccgc gatccggact ccggatcgtg ccggtagcgt ttcgccaggc
     7861 cgccccggat ccggacctcc tcggcgacgt ccagcaggcc ggcctcgtgc aaccgccgca
     7921 ggtgatagct gacattcgct tgtgtctcac cgagttcgcg cgcggcttcg gccgcgctca
     7981 tcgcgacacc ggtcaaaagg gacagaatgc gcagccggac gggatgggcg aggacgcgca
     8041 gctcgtcaag gccgggggac tcgggcatgg gacgagtatc gactgccggg acgcgaacca
     8101 tcaaacagtt atttgggggt gtcattggcc gggagatccg gtgcggtgat cggatgtctg
     8161 tgctctgctg gagacatgcg tacgacgaca ttgggcagga gcggtctcga ggtgtcgagg
     8221 atcgctttcg gcacttggca gctcggcggg gactggggtt cgttcgacga ggacgcggcc
     8281 atcgcggcga tccaccacgc ccgcgaactc ggggtgaact tcttcgacac cgcacaggcc
     8341 tatggtttcg ggaagtccga agcgatgctg gggaaggcgc tgcgcgagga actcaagcgc
     8401 gaccgagaca acctcgtcat cgccacgaag ggcggtatca agcctcgttc cgaacggccg
     8461 cgcgactcgc ggcgcgagtg gctgacgcag ggcatcgagg acagcctccg cttcctcgac
     8521 gtcgaccaca tcgacctcta ccagatccac tggccggacc cgggcactcc ggccgaagag
     8581 acggcgggga tcctgcagga gttcgtcgac gccgggaaga tcaggcacgt cggtgtctcg
     8641 aactacgacg ccgcggcgct cgccgccttc gacaagaccc ggcccgtgga aaccctgcag
     8701 ccgccgtatc accttttccg ccgcgacatc gaaaccgatc ctctcccgta cgcgcgtgag
     8761 aacgacatcg gcgtgctcgt gtacagccca ctcggcagcg gactgttgac cggatccttc
     8821 acgccggaga cgacgttcga gaagaccgac tggcggtcac ggtcgtcggc gttcaccggc
     8881 gagaccttcc ggcgcaatct cgccgtcgtc gaccggctcg aggaattcgc ggcggacaaa
     8941 ggaatctccg tcagtcagct cgcgatcgcg tggacgctcg cccagccggg tgtgcacgtc
     9001 gcgatcgtgg gcgcgcgcaa ggcgaccaac atcgaggcca gcctcgccgc cgccggcatc
     9061 gacctgaccg cggacgatct cgcggagatc gaccggctca cggccgacgc cgttcccgtg
     9121 tcgggcgcga gccccgaagg tgtcgcctag agcttgaatt cggtaccgtc caagggaagc
     9181 gaggcgccgc cgtccagcac ggcggcgtgc tcgggcgacg ccggatcgac caccgggttc
     9241 gtgttgttca gatgtgtgta cacccatcga gggccaggcc ggagcttcgc gaggctgccg
     9301 gtgatcggca gatggcccat cgccaattga gcgcctgcct cggccgcggt ggcgcccgac
     9361 atttcttccg gtgcgtagaa ggtcccgtcc agcagtacca gtccggcgtc gcgggtgaag
     9421 tcgtcgaatc cgtccggcca gctcttcagg cagggcgcgt atacgaagac gccgccggtc
     9481 tcgacgtctt cgatccggta cgccaccacc caagggccgt cctcagtgga cgaacgtgcg
     9541 tacttcggcc gcttgtcact cacgggaagc acactcaccc ggagaccgtc gatagagact
     9601 tccgacgacg gcagccagtc ccagccgccg taaccgtcga tgatcgaccg cgcggggaac
     9661 tgctcggaga gggcgcccag cacggcgtcc ggtgcccaca cgggaaggcc gcccgcttcc
     9721 cgcaggagaa gcagcccgag tgaatggtcg agttcggcgt cggtcagcaa caccccgcgc
     9781 agcgggattt cccgaggccc cggcccggca cgcaaaggcg gcgtggcgag gatctgcgca
     9841 cggatgtccg gcgacacgtt gagcaggtac cagccctcgc cgtccgcgct caccgccaca
     9901 cagtcctgcg tgcgcggtgg cgcgcccgaa acgcacaacg cgcaggcaca gttccactgc
     9961 gggaagccgc cgccggccgc ggtaccgagc aggatcaccc tcatcgtggc gtccgcatca
    10021 cgagttcgtc cgtcttcgcc tgcgtggcga ggatgaggtc gaccacgccg cgttgcgggg
    10081 aacgcgagca caccggatcc gtcgccgagg cgtcaccggt gagctggaac gcctggcacc
    10141 ggcagccgcc gaagtcgatc ccgcgccgat cgcaagtgcc acaggtgtcc ggcatccagt
    10201 cctcgccgcg gaaggcgttg aaggagccgg attcgtacca gatccacgcc agcggcttgt
    10261 cgcgcacgtt gtcgaattcc agtgtctcga tcgccgtcga cgcggggcac ggcagcacgt
    10321 tcccgtcggg cgccacggtc aattgccgtg cgccccagcc gtacatgcac ggcttcgggt
    10381 acttctcgta gtagtcggcg acgacgtaga tgatctccat acggccacgc agccgttcgg
    10441 tggccgcgcg cacgatcggc tcggcctcgt cgagctgacg ccgggtcggc atcagggcgt
    10501 cgcggttgcg cagggcccag ccgtagtact gcgtgttggc cagttcgagc cggtccgcgc
    10561 ccatttcctc ggcgaggtcg atgatcccgg cgagctgatc gtgattctgc cggtgcagga
    10621 caacgttcac cgacagcggc agcccggcct tcttgatcag tccggcggcg atcagtttac
    10681 ggtcgtgcgc ccgcgccccg gccagccggt ccgcgcggtc ggcggtcgcg ccctggacgg
    10741 acagctggat atgcgcgata ccgcgttcgg cgagatcgtc caatctggac tcggtcaggc
    10801 cgagcccgga ggtcaccagg ttgacgtagc atcccagttt cgtggcgtgt tcgaccagga
    10861 tgggcagatc cggtctggcg agcggctcgc cgccggacat gtgcacctgt agcacgccga
    10921 gttcccgggc ctgggtgagc accgaaagcc attcctctgt ggacagttcg ctgtcgcgcg
    10981 tgatcagttc gaccggattc gagcaatacg ggcaatgcag cgggcaacgg tgggtcagct
    11041 cggcgagcat gcccagtggc gccgcggtgt tcaggtccat tcgacgatcc gccgttctcc
    11101 cagccgggac aggacgtcca ggatctcttc ctcgcgcacc ccggtgtagc gctcgcgcag
    11161 ggccgaagcg attcccgcga cgtcttcgac cccgtcgcag agggaaagca cggccgcggc
    11221 ggtcggattc gggacgagca cgccttcggg gaacaaaaga acgtgcgttt ccctggtctg
    11281 gtcgtagctc agccggaccc cgcgccgcag ccgcggcacc gagcccggtt cgagggtggt
    11341 catggctact ccttggccgc gcgctcgatg gcgtcgagca tcgaccagag cacgtcggtc
    11401 ttgaacgaca gcgcggcgat cgccttgtcc tgctgctcgc gggtgacggc atgccgcacg
    11461 acgatgtcga gcgtgccctt gccctcgccg gagaccttgt cgatgcgatt ggtgaaatag
    11521 gcgaggtcgt cgggcgcgat ccacgaatag ttcgccagca tgtcggcgac ccgccgctgc
    11581 atgagatggc cggcgaacat ttcggtcagc cccgaggcga ccgcttcgag ccaaggtttc
    11641 gtgcgcgcga aattcacgta ggcgtcgacg gcgaaccgca caccgggagc gacgtgccgc
    11701 tgatcgagaa cctcttcgcg gtcgagcccg accgcggtgc acaaccggag ccatcgctcg
    11761 attccgccgt caccggcctt cgtcccgtcg tggtagacga tgcggtcgag ccattcccgg
    11821 cggatctccg gcaacgggca gttgctgatg atcgccgcgt ccttctgcgg gagaacgcat
    11881 tggtagtacc agcggttcgc ggcccagatc ttcagctcgc gcgccgacag gtggccttcg
    11941 tgcatccgca cgtggaacgg gtgggtgccc caatagcggt ccgaaagccc gcgcaacgcc
    12001 gcgatgaact ccgacgacga catgagggac accgtgcagc ctccgggtgc tcgctgatcg
    12061 ggacggcggc ggtggtgggg gacggccccc accaccggat gcggattcag tcctccatac
    12121 gggccgcgta agcggtgacc tccatggggg tttcgtattc gacgaagtcc ggggtggtcc
    12181 attcctcgat catttccgct cctaacgtcc acatgggatc gaacagggcc gagccaccgt
    12241 caccctagaa gcgcgtcgcc gattctgacc agacgtaagg accgacttcc ggcggtattt
    12301 tgtaaagttt cctacctatg gccgagcgag cggggcgggc cgtcacgctc ggcgcacaca
    12361 acggttaacg gccggtggat ccgatagccc gtccagtggt gtccgattcg ttccgttgtg
    12421 tgtttctatc caactcgacg ggtgcacccc gaccacccgc ctaccgccgc catccgagct
    12481 ccaggggtgt agctgaagga ggcgtcccca tgtccttacg tcgtactctc gccgccacgt
    12541 tcgccgcggc gacggccgtg tcgatcgcgg ccgtcccggc ggccgccgaa gcgccgccgt
    12601 cgacacaggc gatcagacag ctcgcgagcg gcctcaacca ggcctggtcg atcgacttcc
    12661 tgcccgacgg caccggcgtc ttcacgcaga aggacgccag gacgatcagc aagatcgaca
    12721 aggcgggcaa ggtcaccacg gtccagacca tccccggggt gagcgtcacc gcggaggccg
    12781 gcctgctcgg catcgccgtc tcgccgaact acgccaccga ccagacggtc ttcatctact
    12841 acacgaccag ctccgacaac cggatcgcca agctgaagct gggccagacg ccgacgccga
    12901 tcgtcaccgg catcccgcgt ggttcgcagt accaccacgg cgggcggatc cggttcggcc
    12961 cggacggcta cctctacgcg gggaccggcg acggccagaa cggcgccaac gcgcagaaca
    13021 agaactccct cggcggcaag gtcctgcggg tcaccaccga cggcgcggcc gcgcccggca
    13081 acccgttcaa cagccgcgtc tactcctacg gccaccgcaa tgtccagggc ctgacctggg
    13141 cgaacggcca gctgtacatc tccgacatcg gcgcgaacaa gctcgacgag ctgaacaaga
    13201 tcgagccagg caagaactac ggctggccga cctgcgaggg cccgtgcaac acggccggga
    13261 tgaccaaccc ggtcaagtcc tggccgacgt cctcggcgac gccgagcgga ctcgcggcct
    13321 acaagggctc gctgtacatg gcgtccctca agggcggcac gtacaagctc gacctgaacg
    13381 gcaacggcgg caagctctgg accaacctcg gccgcacgcg tgacgcgacc gccggcccgg
    13441 acggccagct gtacacgatc accccgggtg ccctctacgt ctccgacggc agctaactct
    13501 caagggtctc ccgcgcccgg cggagacttc accggcgcag cctctttcgg gccggaggcc
    13561 cacgaaaacg ttttgcgggg cgccatgtac atgtggccag gcgccccgcg gaacgttctc
    13621 gtcagctgcg cccgatagac accgagtgtt cgagttcggc gagcgcggtg tccaggtggg
    13681 tcaggatgcg ctgcagatgc ggcacgctcc ggcggcagcc ggtgatgccg aagtccaggt
    13741 tgtccccgtt actggtcagc gtgatgttca gcgcctgccc gtccagcagc accgacgccg
    13801 ggtagatgcc gtcgagcgcc gcgccgttcc agtacatctg cttgcgcggc cccgggacgt
    13861 tggagatcac caggttgaac ggcggtttcg tgttgttcac gaaaccgggg atcggggaca
    13921 cgccgagctg ggcgacgttg atccccgaca gcagcagcgt ctgcagcggg gtcagctccg
    13981 agaagagctt cttcccgttc cgcatggacg cgttgatcgt cgccagccgg gcggccggat
    14041 cggtcagatg ggtggcgagg ttgcacagca gcgcgccgat gttgttcccc gccgcgtccc
    14101 cactgtcctt gcgccgcaag gaaaccggga ccatcgcggt cagcggcacg tcggggagcg
    14161 cgttctgctc catcaggtag tcgcgcagcg cgcccgagca catcgcgagg acgacgtcgt
    14221 tgcgcgacac cccggcggcg gtcgccacct tgcgcacccg gtccagcgac caggactgcg
    14281 cggcgaaccg ccgggccccg ccgatcggca cgttcagcat ggtcttcggc gcctgcgccg
    14341 gcagcgtgag cgtgtgctcc tggaacgcct cacgcgccac cttcatcgcg gcgggcgcga
    14401 taccggcgag ctggttgacc gtcttgccga aggtgcggaa caccgagggc gacaccttgc
    14461 cgttgcggcc gccgtcgggt ttggcgcgcc gcccccacgg cggcgggcag tcgaggtcgg
    14521 ccgggtcgtc cgacaacgta ccctgcagat gacgcagcgc cgagacgccg tccatcagcg
    14581 cgtggtggat cttgctgtag atcgcgaacc ggccgtcctg caggccctcg accaggtgga
    14641 tctcccacag cgggcggtgc cggtcgagca gggtgctgtg ccagcggccg gtgagctcca
    14701 gcagctcgcg gatccggccg ggctggggca gcgcggaatg ccggaagtgg tagtcgagtt
    14761 cgagatcgtt gtcggtcgcc cacgcgacgt ggcccgcggt gttgaccggc cgggcgggac
    14821 ggcgccggaa caccgagcgc atgttgtcgg aatcgagcag gctcttgcgg acgtcacgca
    14881 gatagtcgtc cccggcgccc tcgggtttcc tgaacagttg cagtccgccg acgtgcatcg
    14941 gatgttcgcg cgtctccacg agaaggaaca tcgagtcggt cacgggcatg aacggcatcg
    15001 tcggaccacc ttccgcgcgg cggcgtgcgg acacgccggg tgccagagtc tacgcacacc
    15061 gaaaccacct tctcacgctt tagggtggct cacatgacga tcccgaccga tccgcagacg
    15121 ggtgagttcc gtcggtccgc caaccatttc gacgcgcgtg tcaccgccga cggccgcgac
    15181 ggctggccgg tggaggcggg gagataccgg ctggtggtca gccgggcctg tccctgggcc
    15241 agccgtggcc tggtcgtccg gcggttgctc ggcctcgagt ccgcgctgag cgtggccgtc
    15301 gccgacccga tccaggacga gaagagctgg cggttcaccc tcgatcccgg tgaccgggat
    15361 ccggtgctcg gcatcaagta cctggccgag gcctatcacg ccgcggatcc gcactacatg
    15421 ggcgggatca gcgttccggc cattgtggac attccgagca aacggctggt gagtaacgac
    15481 tatcctcaga tcaccctcga tctgtccacc gaatggacca gccttcaccg gcccggcgcg
    15541 cccgaccttt acccagagcg acttcgcggc gagatcgacg acgtcaacgc gggggtctac
    15601 gccgacgtca acgccgccgt gtaccaagcc ggattcgcga ccaagcaggc cgcgtacgag
    15661 gacgcctaca cccgcctctt caccatgctg gacgtgcttt ccgaacggct cgaaaagcag
    15721 cgctacctcg tcggtgacac gatcaccgaa gcggacatcc ggttgttcac cacgctcgtc
    15781 cgcttcgacg ccgtctatca cgggcacttc aaatgcaacc ggaacaagct gaccgagatg
    15841 ccggtgctct gggcctacgc tcgcgacctc ttccaaacgc ccggtttcgg tgacaccgtc
    15901 gacttcgacc acatcaagcg gcactaccac gcggtgcacg agcaggtgaa cccgacgcgg
    15961 atcgtccctt tgggaccgga cctgtccggc tggacgacgc cgcaccaccg ggagcagctg
    16021 ggcgggcgcc cgttcggcga cggcacgccg ccgggaccgc cgcccgagga cgaacggatc
    16081 ggggacacgg atggagccac tgcctgaacg caccgacgtc gtcgtgatcg gcggcggcat
    16141 catcggggtc gcctgcgcgt accggctcgc cgccgccggc gtcccggtcc tgctgctcga
    16201 acgcgacatc ctgggcgcgg gctcgacggc gaaggcggcg ggcgggatcc ggtcgtcgtt
    16261 caccacccgg gtcaacatcg agatcgggct gcgcgggctc gccgagtacg cctccttcgc
    16321 cgagacgtac gggaccgaga tcgatttccg tcgcgacggc tacctgtacc tggtcaccga
    16381 ccccgcggac ctgcccgaat tcgaacgctg tgccgaactg cagaactcct acggcgtccg
    16441 cagccgtctc gtcgagcccg ccgaagcgcg gcggttctcc ccgctgatcg acaccgaggg
    16501 cgtcgtcgcg gcgctgtggt cgccggacga cgcgaaggcg actccggact ccgcggtcca
    16561 gggctacgcg cgggccgctc gcgcgcacgg cgcggtgctc aggaccggcg tcgaggtcgt
    16621 cgggatcgaa cgcgacggtg acgtgatcac cggtgtccgg acctcggccg gtttcgtgcg
    16681 gacggacgcc gtggtctgcg cggcgggcgc gtggtcgggc cggatcggcg aactcgccgg
    16741 tgtcgacctc ccggtgcggc cgttccggcg ccaggtcgtg ttcaccggac cggtcgcgga
    16801 cctgccgggg tcggtgccgc tgaccatcga gatgccgtcc gccttctact tccaccgcga
    16861 gggcgccggc ctggcgatgt ccttctgcga cggtgatggc gaccccggct tcgacacccg
    16921 ctatcagccg ggcgagtggc tgccgaaact cgccgagatc gccgcgcgcc gggtgcccgc
    16981 ggtgctcgac gcggggatcc gcggcggctg ggccgggctg tacgagatga ccccggaccg
    17041 caaccagatc gtgggggaaa gcgtccacct gtcgcggttc ttctacgcca ccggcttctc
    17101 gggacacggg ttccagatgg gcccggcggt ggctgagctc gtgcgggacc tctacctggg
    17161 gcgggtctcg gagatcgaca tcagcgggct cgacgtccgc cggttcgccg gcgaccgggc
    17221 cgtgacgagg gagcacaaca ttgtctgagt tcgtcgaagt caccaccgag gccgagctgc
    17281 gcgagatcct gccgccgccg ctcgaacgga ccgcgaacaa ggcgcgcccg aagctgcacg
    17341 agatggaccg ccagtggctc gcggagtcgc cgttcgtgct gatcgcgacg tcggcggcgg
    17401 acgggacctg cgacgtctcg ccgaagggcg acccggccgg gttcacgctg gtactggacg
    17461 acacgcggat cgccatcccg gagcgtcccg gcaaccggcg cgccgacggg ttccgcaacg
    17521 tgctgtcgaa tccgcacgtc gggctgatct acctgatccc cggccgcggc gacacgctgc
    17581 ggatcaacgg ccgcgcgcgg ctcgtgcggg aggcgccgtt cttcgacgac atgatcgtga
    17641 acggcaaccg gccgaagctc gcgctggtcg tcgacatcga cgagatcttc caccactgcc
    17701 agaaggcgtt catgcgctcg aagctctggt cgccggagag ctgggcgccg gacgcgctgc
    17761 cgtcgcgggc ggtcatctcc aagaccttcg agcggccgga ggacagcctc gccgacctgg
    17821 aggcgtatta cgctcctgcg gcttactcgg ccgggttgta ctgaagccgg ctgcgcgtga
    17881 aggccccctt ccctcggctc agccgaggga agggggcctt cacgcgcagt ggccggttgc
    17941 tggatcgttg aacgatctgg cactatcggc ggggtgcctg cacccaccgg agcccagcat
    18001 ctcgccgacg cgctgaccgc tctggggacc gaagtcgtct tcggtctccc cggggtgcac
    18061 aacctgccgt tatgggaggc gctggccgat acggacatcc ggctggtggg ggtccggcac
    18121 gagcagacgg cggggtacgc ggccgacggc tacgcccgcg cgacgggcaa gctcggcgtc
    18181 gcgctggtga ccacggggcc cggcgcggcg aacacgctcg ccgccgtcgg cgaggccatg
    18241 gcgtcggccg cgccggtgct gatcatcgcg accgacatcc cgtccacgct gcggcggccg
    18301 ggcgtggtcc gcggcgtgct gcacgagacg tcggaccagc aggcgatgtt cgcgccggtc
    18361 accaaggcgg ggttcaccgt caccagcgcc gaccaggtcg ccgccaccct ccaccgcgcg
    18421 gcccggctcg cgctccagcc gcagagcggg ccggtctacc tcggcatccc ggccgacttc
    18481 ctgtccgagc ggacgcccgc gcgccgtccg ccgcggtcgc acgcccggcc cgccgagatc
    18541 ccggatctca ccgaggccga ggcgctcctg gccaccgccg accgaccgct gatctgggcg
    18601 ggcggcggcg cgctgcgcgc cgaggcgggt gaggcgatcg ggaaactcgc cgaacggctg
    18661 gccgcgccgg tgctcaccac gttcggcgcc cgcgggctgc tgccggtcga tcatccctgt
    18721 ctcgccccga atccggtgca cgcgcccgag gttggcgcgc tgtgggacga ggccgacgtc
    18781 gtcctcgcga tcggcaccga cttcgacggg ctgatgacgc agaactggct gatgccgaag
    18841 ccgaagaaac tcatcgcgat caacgtcgac ccagacgacg cggccaagaa ctaccagccg
    18901 gacatcacgc tggtcggtga cgccagagtg ctcgtcgagg cgctgaaggt cgaggaacgg
    18961 ccgggtatcg cggcactcgt cggcaggctc gccaaggtct ccgcccgggt acggcggcgg
    19021 atcaaggagg aggaaccaca ggccgcggac cttctgtcta ctttggatga ggtgttgccg
    19081 cccgacgcgg tcgtggtcgc ggacatgtgc gtggccgggt actggatcgg cgggttccat
    19141 cgcgtccgcg cgccgcgcaa gctcgcgtac ccgatgggct ggggcaccct cggctacggc
    19201 ttccccgccg cgctcggcgc gagtgccgcg ggcgccggcc gcgcgatctg cgtcagcggt
    19261 gacggcggtt tcctgttcgg ctgcggcgat ctgcacacgc tggcgcagga acagttgccg
    19321 gtcacggtga tcctcgtcga cgacggcggc tacgggatgc tccggtacga ccaggatcgc
    19381 gcgggattgc cgcgtcgagg ggtcgactgg gacagtcccg atttcgtcgg cctggcgagg
    19441 gccttcggcg tacacgcgga ccgggtttcc gggttcgggc gggcgttccg gcggctgctc
    19501 ggtgaattcg tcgagtccga cgagcccaac gtgctggtcg tgcgcgcgaa gatggcaccg
    19561 ccgctcaaca cctcgccgcg ctggtaccgc aaggaatccc cgtgaccggg ctccgcgtcg
    19621 gcgtggacat cggcggcacg ttcaccgatc tctgcgtgct cgacggcacc ggtctcgtgg
    19681 cggtcggcaa ggtgctgacg acccacgacg aaccggcccg cgcggtcgag gaaggtctca
    19741 agaggacact cgccgacgcc gggctggacg cgggagacgt cgagcagttc gtgcacggca
    19801 ccacgctggt gacgaacgcg ctgatcgaac gcaaaggtgc tcgcacggcg ctgctcgcga
    19861 ccgccggttt ccgcgacgtg ctggagatgc ggcgcgagca ccggtacgaa ctgtacgacc
    19921 tgctcatcga attgcccgcc ccgctggtgc cacggcacct gcggttcgat atccccgagc
    19981 ggatcctcgc cgacggtacc gtccacacag gactggacga ggagtacgtc gcgcggctcg
    20041 gccgggagct cgacgccagg ggcatcgacg cggtggcgat ctgtttcctg cacgccttca
    20101 cgaatcccgc gcacgaaaga cgcgccgccg aggtgctgcg ggaggtcgcg ccccggctgc
    20161 gggtcgcgct ctccagcgag gtcgtccccg agatccgcga attcgagcga atgtcgacca
    20221 ccgtcggatg cgtctacgtc caggatctca ccgaacgtta tctgcgtgat ctggaaaggc
    20281 gcctgcacga ggccggggtg cggccgtcgc cgcacatcat gctgtccaac gggggaatcg
    20341 cgacggtgga caccgccgcg cggtacccga tccgcatcct cgaatccggt ccggcgggcg
    20401 gggcgctggc cgcgtccgcg atcggtgccg cggccggtgt cgaggatctg ctggccttcg
    20461 acatgggtgg cacgacggcg aaactgtgcc tgatctccga tggcgcaccg ctggtgacgc
    20521 acgagttcga agtggaccgg aagtaccggc tgctgccggg ttccgggctg ccggtcaagg
    20581 ttccggtcac ggacatgatc gagatcgggg tcggtggcgg ctcgatcgcg cggatcgacg
    20641 cgctcggcct gctcaccgtc ggcccggatt cggcggggtc cgagcccgga ccggtctgtt
    20701 acgggcgagg cggtgccgag ccgaccgtga cggacgccga cctcgtgctc ggttatctgg
    20761 acccggggta tttcctcggt ggcggaatga ctttggatct cgaaggcgcg cgcgaggcca
    20821 tccgctcgaa gatcgccgga ccgctcggtg tcagcgtcga agaggcggcc tggggtatcc
    20881 acaccagcgt caacgaggac atggcgaacg cggcccgcgt ccacgccgtc gagcgcggca
    20941 aggatccggc gaagctcccg atgttcactt tcggtggcgc cggcccggtg cacggcgtcg
    21001 gtgtggcgag ggcgctcggc gcgccggagg tggtcgcgcc gcccgccgcc ggagtgctca
    21061 gcgcggcggg attcctcacc gcgccactgg ctttcgactt cgtgcgttcg gcgcgctccg
    21121 cggtactgga catttcctgg gaacaggtcg acgcgcggtt cgccgagatg gaggccgagg
    21181 gcgcggagct cctggagaag tccggtgtgg acggttccga cgtgacgcac cgccggatcg
    21241 ccgagatgcg gtattccggg cagggttacg agatccgcgt gccggtcgga cgggggagct
    21301 ggccggagtc gctcatcgac gcgttcaccg agacctatcg gacgctgtac cggcgggccg
    21361 ggccggacgt cgcgatcgag gtgctgaact ggcgcgtcgt ctccagtggt cccacgcccg
    21421 aggtcacgct gaaactcgcg ggcgccgagg ccgagggcga cgcgcgcaag gggagccgcc
    21481 ccgcgtactt ccccggcgaa ggtggatttg tcgacacggc ggtgttcgac aggtaccggc
    21541 tcaagccggg tgatcgcgtc gaggggccgg ccatcgtgga ggaacgcgag tccaccgtgg
    21601 tcgttccgcc gggtgcgcgc tgtgtcgtcg gcgcggacgc cagtctggtg gtgacggtat
    21661 gagcgtcgac ccgatcctgg tgggggtgct gggaaaccgg ctgcactcca tcctggccga
    21721 acagcagaac gcgctcgtgg acacggcctt ctcgtccgtg gtgcgggaat cgctcgacct
    21781 cgcctgcgcg gtgttcgact cccgcggcga gatgatcggc cagtccgtcg gcgggacacc
    21841 gggccatatc aacgcgatgg cgaccggcat gcggcatttc gtcgccgcgt acccgccgga
    21901 gacactggag cccggcgacg tcctgctcac caacgacccg tggcagacgg ccgggcagat
    21961 caacgacatc accgtcgcga cgccggtgtt cctgcgggga cggctggtcg cctggttcgc
    22021 ttc
//
