LOCUS       NZ_CP102415            10390 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Amycolatopsis roodepoortensis strain ZEL-1 chromosome, complete
            genome.
ACCESSION   NZ_CP102415
VERSION     NZ_CP102415.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis roodepoortensis
  ORGANISM  Amycolatopsis roodepoortensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Biodegradation of petroleum hydrocarbons by Amycolatopsis orientalis
            ZEL-1, genome analysis and its potential in plant protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) College of Life Sciences, Sichuan Normal
            University, No. 1819, Section 2, Chenglong Avenue, Longquan
            District, Chengdu 610101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024628825.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 06:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,064
            CDSs (total)                      :: 8,994
            Genes (coding)                    :: 8,928
            CDSs (with protein)               :: 8,928
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 44 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5996511
            Orig. end                         :: 6006901
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="22"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(234..248)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     misc_feature    235..249
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    459..479
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.8"
                     /tool="antismash"
     misc_feature    complement(767..785)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.16"
                     /tool="antismash"
     misc_feature    2402..2413
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            3186..3677
                     /gene="ectA"
                     /locus_tag="NQK81_RS28675"
                     /old_locus_tag="NQK81_28675"
     CDS             3186..3677
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="NQK81_RS28675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28675"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_257484000.1"
                     /transl_table=11
                     /translation="MSGKHLIENPTKADGAALWRIARDSKKLDLNTPYAYLLWCHDFAD
                     TSVVAKVDGTPVGFVIGYRKQDVGFVWQVAVDASQRGKGLAGALLDELFGRLTAQGVRY
                     LETTITPDNEASIRLFASFAKRWNATVERQDLFSATDFPAEEGTHEREDLYRIGPLSSQ
                     "
     gene            3716..4966
                     /gene="ectB"
                     /locus_tag="NQK81_RS28680"
                     /old_locus_tag="NQK81_28680"
     CDS             3716..4966
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 1.1e-73.
                     Score: 240.2. Matches aSDomain:
                     nrpspksdomains_NQK81_RS28680_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033455.1"
                     /locus_tag="NQK81_RS28680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28680"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_126730308.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-74, bitscore:
                     250.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKPYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTLQEKILRPRDLDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTDDE
                     LTKGLAIIDEAVATVLN"
     aSDomain        3779..4789
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS28680_Aminotran_3.1"
                     /evalue="1.10E-73"
                     /label="NQK81_RS28680_Aminotran_3.1"
                     /locus_tag="NQK81_RS28680"
                     /protein_end="358"
                     /protein_start="21"
                     /score="240.2"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKPYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTLQEKILRPRDLDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARVEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     gene            5001..5390
                     /locus_tag="NQK81_RS28685"
                     /old_locus_tag="NQK81_28685"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="NQK81_RS28685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28685"
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            5415..6314
                     /gene="thpD"
                     /locus_tag="NQK81_RS28690"
                     /old_locus_tag="NQK81_28690"
     CDS             5415..6314
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631927.1"
                     /locus_tag="NQK81_RS28690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28690"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_126730310.1"
                     /sec_met_domain="PhyH (E-value: 4.6e-31, bitscore: 107.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEHLPRTHPTVWGTEADGPLDAATLA
                     NHETRGYTVVDQLLSPGEVQTYWQELVRMSSGDHSGDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPV
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPEDNYKSSLKEQRVGVPSEADI
                     TALAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFLVFNSVENALREPYAA
                     IEPRPAFIAGRDSTPLSR"
     gene            6868..9657
                     /locus_tag="NQK81_RS28695"
                     /old_locus_tag="NQK81_28695"
     CDS             6868..9657
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="NQK81_RS28695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28695"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_257484001.1"
                     /transl_table=11
                     /translation="MAESGSPARFQLLGPVRLLDGDRPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRGIQGDHPDGARILTAPPGYQLTV
                     DPERIDVHRARSLLERASAEEPEKASALLTEALALWQGPALAGVPGSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLVRANPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKVVAEPKAEAAPVQQLPPAVPNLAGREAD
                     LAWLDELAERAEAGETAVGVVTGTAGIGKSSLVVWWAHRAGRRFPDGILFASLRGFDPH
                     HPPLEPADLLTQFLLGLGVPAEGVPEQVHERVALYRSMIAGRRMLVILDNARSAEQVRP
                     LLPPGSRSMTLVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGFDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWTAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPPEVADTFLRLGVVQCVSAGSHLTAAIGGITVAEARRHLRVLAAHNLLAETGRDS
                     FVPHDLVRLFLRELAENELDEADREDVLSRSVRFYQAVADRARRKMLRIVDPLDFTGEL
                     TDAQTPPIGSFDEAQDWFTAEWENLIEVLEAARAAGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVEIGLEAAERSGDVLGRCWMLISRCAIALTFELPQGCLADAERALELATAIGDD
                     RLTISANIHLGSALTLLGRHDEAIRTLRRVVEETERTGDLELRGQALNNCAEAEKRAGR
                     FIEAIGHQVASLEIDRTLGDESYVVVSLNNLAELSLRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRAGSDVDGAREQFALALKILADANPKLAALVRAELTDLGESP"
     misc_feature    10205..10220
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.95"
                     /tool="antismash"
     misc_feature    complement(10302..10315)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.99"
                     /tool="antismash"
ORIGIN
        1 ggggagcccg tggccgcagg agatccggct ggcgatgacc atggtgggcg gggcgagcct
       61 cgcggtctgg atgggcgggg tcgccaccga gacctcgcat ctgttgcagg cgtcgagaac
      121 gccggaatcg aaaagcccgt accgggccct gctcgatctg ctcaacgcca ccgtctcgct
      181 cgacgtgctc accggcacga gcgccggcgg gatcaacgcg gcgtgcctcg ggctggccga
      241 ggcgttccgg tcgagtccgc aggtgctgcg cgacacgtgg atcagcacgg gctcgctcga
      301 caacctgatc cgcgaccccg gcgagaagga gccgcgttcg ctgctcgacg gcgacaaggt
      361 gctgctcggc gatctcaagg acgcgctgca ccggatcacc gacaacgcga cggtgaaacc
      421 cgactgtccc gacatcaccg tgctgctcac cggcacgatg atcgacggcg agacgaccag
      481 gttcgacgac gcgctcggaa acctggtgcg ggacaccgaa catcgcctcc tgttccgctt
      541 cgacgctcct ttgtggacgg acgacgtggt cggcccgctc gcgctcgcgg cgcggtccac
      601 cgcgtcgttc cccggcgcgt tcgagctctc ccggatgccg atcggcgaga agaccggccc
      661 gctgcatccg gacatgacca aatacaccga cgtcacccgt tcgcattggc tcaccgacgg
      721 cggggtgctg ctgaacaagc cgctgcgacc ggcgttgcgg gagatcttcg agcggcagtc
      781 gcattcggac gtccggcggc tgctgctgta cgtggtgccg acggcggaac gcgaagccga
      841 gcgggtcgcg gtcgatccgg agcggccccc gctgctgggc agcgcgctga gcaaggtcgt
      901 cggcaccgtg ctgagccaga cgatcagcgc cgagctggag gacctgacca ggcacaacga
      961 cgccgtcgtc cggaccaggg gcacccgcgt ttcccttgcc gcgatggggg ttcgcggcgg
     1021 cccggactcg ctggtcgacc agcggctgat gaacgactac cgcgaccggc gggtgcagga
     1081 ggacgccacc gcgctggtcc gcgaggccac ccgcaggctc tcactgtccg atgtggagga
     1141 tcagggccgt cagtgggcgt ccggcacggc ggcgcaactg cgcgcggccg cggcggccgg
     1201 gctgcgggac ggcctgccca ccgagccgcc gaaggacagc tgcgaactcg cggacctggt
     1261 cgccttccgc accaccgcgc tcgacgattc ggtggccacc gggctgcaac tggtgaacgc
     1321 gggcttccgg ctcgatccgg cgccggagca ggcggtgcag ctcaaccgtt gccgcgtgct
     1381 gctgcacgaa gcgcggcaca aggccgcgcg gggcaaacgg ctcgcgggct gggtcaccga
     1441 acaggagccg cccgcctccg acgtcacgct ggctgcctgg atcgaaggcc tcgccaagga
     1501 atgggccgcg ctcggccgtt cggacacgct gaaggaggcg tggccgctcg tcgtcgccgc
     1561 cctccggcag gccacaccga tccttttgcc gctggcacaa gcgaaacccg acaccgaagc
     1621 cgccgacacg gtcagcacgc tgctggcctg gaccgggctc acctccgacg acacgagcgc
     1681 cggcgatccc attgtcacct cgcggctcgt gcgcctgcac atcgcgaccc gggggctgct
     1741 cgcgcagccg ccctcggtcg accaacgggt cgatctcgtc caggtcagcg cggattcgcg
     1801 gacgctgctg gacatgaagc ggcgccgttc gtgggacaag ctcaccggga tgcaggccga
     1861 ctacttcggc gcgttctaca aggcgtcctg gcgtgcgaac gactggatgt ggggccgcgt
     1921 cgacggcgcg ggctggctcg tgcagtgcct gctggacccg aaacggttgc gcctgctgcg
     1981 cgacgtcgtc ggacgtgagg cgttccggac gcaggtgcgg gacaccttca aggagatcgg
     2041 ctggcggcag cccggcacgg aagacgggct gtcgcaagag gaagcggacg ccctgcgcgc
     2101 gcaattggcc ggagagctgg ctttcctcgg cctggacggc gaactggccg atgtggaggg
     2161 agaagccggc ctcccggtca gcatgccggt gacggcgatg gtgctcgcca gggtgcggca
     2221 gctggagatc gcgcgcgagg agctgccgtg tgtcggcctc cactgcggac acgacgcgaa
     2281 aacggccaag gggaacggaa aaccgtcgga acggttcagg cgactcgtcg agaacgagcc
     2341 cgaaaccgac gagcagaccc agcgcgcgtt ccaggcgtgc caggtctccg gcgagcggtt
     2401 cgaacacgaa cgcgggacga tgctcctgac gaagacgctg gtcaaggccg gtgccgccgg
     2461 gctcaacgcg gccgccgggg cgaccagggt gccgaaatcg gtccagcccg cggccacctt
     2521 cgccaaggcc gccgggcgca gcgcgtggtg gatcacgcgc ggggcggcca ccctgccgtc
     2581 gccgtggaac gtgctcgccg cgctgatcac cgtgctggcc gggttcgtcc tcggtggaca
     2641 gggcggcccg gtcctgcagt gggtcggcgt gccggtcgcc gcgggcgccg tcgtgttcct
     2701 ggcggtgagc ctgatgacgt tgcgcaagac gtggcggatg gtgctcacgg tgctgggtgt
     2761 gctggtggtg gcggccctgc tgttcgccgc gttcctgcca ccggtgcgcg acccgctgtt
     2821 cggctggctc ggcggcgtcg tcgcgggctg gcggcgcggc gaggcgccgg tctggtggct
     2881 ggtcgtctgc ctgttgctcg tcctgcccgc catctggacg ccgctcggtt cgctcgtggg
     2941 tcgcaagcgg cgtcgtgaat agcggaatcg gatgccgaat ggtgccctga gcctgcgacg
     3001 aagcggtaac ggttggtatc gtcacatgca gtgagaccgc agcggagcgg ccttacccac
     3061 aagtgctgcg cttcaaacgt taccgttacc tttgtcacgt gtgtttcagg gttgttgcag
     3121 gtagccgcgt tgccgccgcc gcgctgatct gaccgcgccc gaatcctccg gtacgtttct
     3181 tggacatgtc cggaaagcac ttgatcgaaa atccgaccaa ggcagacggt gccgcgctct
     3241 ggagaatcgc ccgcgattcg aagaagctcg atctcaacac gccttacgcc tatttgctgt
     3301 ggtgccatga tttcgccgac acgtcggtgg tcgcgaaagt cgacggcacg ccggtcggct
     3361 tcgtgatcgg gtaccgcaag caggacgtcg gcttcgtctg gcaggtcgcg gtcgacgcgt
     3421 cccagcgagg aaaaggcctg gccggagctc tgctcgacga gctgttcggc cggctgaccg
     3481 cccagggcgt ccggtacctc gaaacgacga tcaccccgga caacgaggct tcgatccggt
     3541 tgttcgcctc gttcgccaag cggtggaacg ccaccgttga acgccaggat ctgttctccg
     3601 cgacggattt cccggccgag gaaggaacgc atgagcggga ggacctgtac cgaatcggtc
     3661 cgctcagcag tcaatagaac gtaaatccgg ggatctcagg gagaaagaaa acgtcatgag
     3721 cattttcgag gaactcgaat ccgaggtgcg cagctacagc cgcggctggc ccgtcgtgtt
     3781 cgacagggcg caggggagct acctctacga cgaggacggc aagccctatc tcgacttctt
     3841 cgccggcgcc ggcgcgctca actacgggca caacaacccg gcgctcaagc gggcactgat
     3901 cgactacatc gcgcgcgacg gcgtgacgca cgcgctcgac atgttcaccg tcgccaagcg
     3961 ggacttcctc cagaccctgc aggagaagat cctccggccg cgggatctgg actacaaggt
     4021 cgtcttcccc ggccccggtg gcgccaacgc ggtcgaggcc gcgctgaagc tcgcccgcaa
     4081 ggtgaccggc aaggaatcgg tcatcaactt caccaacgcc ttccacggga tgacgctggg
     4141 cgccctctcg gtgaccggta actcgatgaa gcgcggtggc gcgggtgtcc cgctggtgca
     4201 cgccaccccg atgccgtacg acaactactt cgacggctcc atcccggact tcctctactt
     4261 cgagaagctg ctcgaagact ccggcagcgg cctgaacgag cccgccgccg tgatcgtcga
     4321 gggcgtgcag ggcgagggcg ggatcaacgc cgcgcgcgtc gagtggctca aggcgctgga
     4381 cgacctgtgc aagaagcacg gcatcctgct gatcctcgac gacgtccaga tgggctgcgg
     4441 ccgcaccggc ccgttcttca gcttcgagga cgcgggcatc aaacccgaca tcgtctgcct
     4501 ctcgaagtcc atcggcggct acggcatccc gatggcgctg acgctgatcc gcccggacct
     4561 cgacgtctgg gagccgggtg agcacaacgg caccttccgc ggcatcagcc cggcgttcgt
     4621 caccgcgacc gaggcgctgc gcgtctactg gagcgacgac gagctggaga agtcgaccaa
     4681 ggccaagggc gagcgcatcg ccgccgcctt ccagggcatc gtcgaggcgt acccggacgc
     4741 gaacctgttc gcgaagggcc gcggcctggc ccggggcatc gagttcgcca acggcgacct
     4801 cgcgggcaag gtctgcgccg ccgcgttcga acgcgggctg ctgatggaga cctccggccc
     4861 cgacggcgag gtcatgaagg tcctcccgcc gctcaccctg acggacgacg agctcacgaa
     4921 ggggctcgcg atcatcgacg aagccgtcgc cacagtcctg aactgaccgc cctcaaccag
     4981 gaacgagaag gagttttccg ttgcttgtca gaacactcga cgaggtcacc gacaccgacg
     5041 ccgacatcaa gaccccgaac tggcgcagca agcgcatcgt gctggccaag gagggcgtcg
     5101 gcttctcggt gcacgagacc acgctgtacg ccgggacggt caacgacttc tggtacgcga
     5161 accacatcga agcggtgttc atcacctctg gtgagggtga gctcgaaaac accgcgaccg
     5221 gcgaggtctt ccagctgaag ccgggcacgt tgtacctgct caacgaccac gacaagcacc
     5281 aggtccggcc caagaccgag atcaagtgcg tctgcgtgtt caacccgccc atcaccggcc
     5341 gtgaggtcca cgacgaaaac ggcgtgtacc cgctcgtcac cgaaccctag aaatcgctcg
     5401 aggaggcgac tgcattgacg ctgaccgaca cccgagtcga cgacagttac ccgacccgca
     5461 tcacgggtaa gcccgaacac ctgcccagga cgcaccccac cgtctggggc accgaggccg
     5521 acggtccctt ggacgcggcc acgctcgcca accacgaaac gcgtggttac accgtcgtcg
     5581 accagttgct gtcgccgggc gaggtccaga cctactggca ggaactcgtc aggatgtcgt
     5641 cgggggacca cagcggcgac gaacgcgtga tcaccgaggc caagaccggt gaggtccgct
     5701 cgatcttcga cgtccacgag accagcgagc tgatcgcgga actcgtccgc gacccgcgtg
     5761 tgctcgaccg ggcccggcag atcctcggct ccgaggtcta catccaccag agccgcgtga
     5821 actacatgcc cggcttcaag ggcaccgggt tctactggca ctccgacttc gagacctggc
     5881 acgccgagga cggcatgccg gtgccgcgcg cggtcagctg ctccatcgcg ctcaccgaca
     5941 actacccgtt caacggcggg cttatggtga tgccgggttc gcagcggacc ttcgtccagt
     6001 gcgccgggga aaccccggag gacaactaca agagttcgct gaaagagcag cgggtcggcg
     6061 tcccgagcga ggcggacatc accgcgctcg ccgccgagta cgggatcgac cagttcaccg
     6121 gccaggccgg ctccgcgctc tggttcgatt cgaacgtgat gcacggttcc tcgaacaaca
     6181 tcacgccgta cccgaggtcg aacatcttcc tcgtgttcaa cagcgtcgaa aacgcgctgc
     6241 gggagccgta cgccgcgatc gaaccgcgtc cggcgttcat cgccggccgc gactcgaccc
     6301 cgctgtcgcg ctgacggaac cgcaggtagc gaaggccatg atggggcgct tgtagccgtc
     6361 ggtgtgcgcc ctgttacgtt gtcgccacgt gagcgggcca gtgggctcgc tcacgtaggt
     6421 gttgtcgacg accgaacggg ttcttcgctc ggggtgagcc cacccggacg gacgtggacg
     6481 ccgatgtccg atgtgggtcg cgcccccagg cggcccaagt cggacatccc ccggctccgt
     6541 gccatcagat cgggactgat ggcacggagc cttttctttt tcgtgagtga atgctgtgtg
     6601 ttcctgaagc gcgtgaaggc ccccttccct cggctcagcc gagggaaggg ggccttcacg
     6661 cgctacccgc cgttggagtc cacagtggac gaaaggtgcc cgcttaggtg cctttgtccg
     6721 gatcggtctg gtcgtgaagg cctccctccc taccctgaaa gtagggaagg aggccttcac
     6781 ggcctcgagt gtcctggttg gcgggctctc cgcgcggtag gttggctgtt ccggtaaggg
     6841 gagagatcgg agggaggggg gcttgtcatg gccgaaagcg gttcccctgc ccgcttccag
     6901 ctcctcggtc ccgtgcgcct cctcgacggc gatcggccgg tccccgtcgg cggtcccggc
     6961 gtccgcggcc tgctcgcgct gctggcgctg aaggtcaaca aggtcgtcgc gctcgacgag
     7021 atcatcgacg cgctgtgggg gcacgacccg ccggccacgg cccgcacgat cgtccacggc
     7081 aacgtctcgc atctgcgccg cgtgctccgg ggcatccagg gcgaccaccc ggacggcgcc
     7141 cggatcctga ccgcgccgcc cggctatcag ctgaccgtgg atcccgagcg gatcgacgtc
     7201 catcgcgccc gatccctgct ggagcgggca tcggcggaag agccggagaa ggcgtccgcg
     7261 ctgctcaccg aggccctcgc cctctggcag gggcccgcgc tcgccggggt gccggggtcg
     7321 gtccgtgcgc ccgaactgga ggatctgcgg ctcgccgtcc acggcgccag ggtcgacgcg
     7381 gacctcgaac tgggcaggca cgcggaactc atcgtcgagc tcagcccgct cgtccgggcg
     7441 aatccgctcg ccgagcgcac ggcgggtcag ctgatgcgcg cgctctacca cgccggccgc
     7501 cgcggagacg cgctcgagct ctaccgcacg gtgtcccggg cgaccctgcg caccctcggc
     7561 gtcgaacccg gcgcggacct gcgctggctg cacgaacgcg tcctcaacga cgacctgccg
     7621 gtcaaggtcg tcgcggaacc gaaggccgag gcggcgcccg tccagcagct gccgcccgcg
     7681 gtgccgaacc tcgccgggcg cgaggccgac ctggcctggc tcgacgaact cgccgaacgc
     7741 gccgaagcgg gcgagaccgc ggtcggcgtg gtcaccggga cggccgggat cggtaagagc
     7801 agcctggtgg tgtggtgggc gcatcgtgcc ggccgacgct tccccgacgg catccttttc
     7861 gcctcgctgc ggggtttcga cccgcatcat ccgccgctgg agcccgccga cctgctcacc
     7921 cagttcctgc tcgggctcgg cgtcccggcg gagggcgtcc cggaacaggt ccacgaacgc
     7981 gtcgccctct accgctcgat gatcgccggg cgccggatgc tggtcatcct cgacaacgcc
     8041 cggagcgccg agcaggtccg gccgttgctg ccgcccggtt cgcggtcgat gacgctggtg
     8101 accagccgtt cccgcctcga cgggctcgcc gtgtccaacg cggccaaact gcgagtgctc
     8161 ggcaccctcg cgcccggcga cgccgtccgg ctgatcgagg agctcgccgg gcccgccggt
     8221 ttcgacctca accacgcgct cgcccggctc tgcggctatc tcccgctggc actgcggatc
     8281 gcgggtgccc ggctcgccgc gagcgcgcag tggaccgcgc aggatctggt cgacgaactc
     8341 ggcaacgaac ggacgaggct ggccgcgctc gacgtcgagg gcccggacga cggtgtccgc
     8401 gcggcgttcg acgtctcgtt ccgggggctg ccgcccgagg tcgcggacac gttcctgcgg
     8461 ctcggcgtgg tccaatgcgt ttcggccggt tcgcatctga cggcggcgat cggcgggatc
     8521 accgtcgccg aggcgcggcg gcatctgcgg gtgctcgccg cgcacaacct gctcgccgaa
     8581 accggccgcg attccttcgt cccgcacgat ctcgtccgcc tgttcctccg cgagctggcg
     8641 gagaacgaac tcgacgaagc cgaccgcgaa gacgtcctgt cgcgttcggt gcggttctat
     8701 caggccgtcg ccgaccgggc ccggcggaag atgctgcgga tcgtcgaccc gctcgacttc
     8761 accggggagc tgaccgacgc gcagacgccg ccgatcggtt ccttcgacga ggcgcaggac
     8821 tggttcaccg cggaatggga gaacctgatc gaggtactcg aagccgcccg cgccgccgga
     8881 cgccacgacg acgtctggcg gctggccagg gtcgcgcaca cctatcgcgc ggtgtacccg
     8941 ttgctggacg agtggacgcg gctggtcgag atcgggctgg aggccgctga gcgatccggc
     9001 gacgtcctcg gccggtgctg gatgctgatc tcccgctgcg ccatcgccct caccttcgag
     9061 ctgccgcagg gctgcctcgc cgacgccgaa cgagcactgg aactcgcgac cgcgatcggc
     9121 gacgaccggc tgacgatctc cgcgaacatc cacctcggct ccgcgctcac cttgctgggg
     9181 cggcacgacg aggcgatcag gacactgcgg cgcgtggtcg aggagaccga gcggaccggc
     9241 gatctcgaat tgcgcgggca ggcgctcaac aactgcgccg aggcggaaaa acgggcggga
     9301 cggttcatcg aggcgatcgg gcatcaggtc gcgtcgttgg agatcgaccg cacgctcggg
     9361 gacgagagct acgtcgtcgt ctcgctgaac aacctggccg aactgagcct gcggatcggc
     9421 gagctcgccg cggccgagcg gtacgtgtgg gaagcggtgg acctgacgat cagcagacgg
     9481 ttcgtcctgc aggagggcgt cctgcggctg acgctgggca gggtgctgcg ggccgggtcg
     9541 gatgtggacg gtgcccgcga gcaattcgcg ctcgcgctga agatcctcgc ggacgccaat
     9601 cccaagctcg ccgcgctggt ccgtgcggaa ctcacggacc tcggcgaaag tccgtgagtg
     9661 atccttagcg gattcttagt ggcctcgccg cgcacgcgcg tagggtccgg ttcagtgtcg
     9721 atcgagccct aggggagggg cacgcggtag tgatcggcgc tcgggcgggc ccttcgcggg
     9781 tcccgtccga agagccgcca ggagcggctc gtggcggtcc ggccggtgga ggtgggggcg
     9841 ataccggccg gccgccaggc ggcacccccg cagggggtca gccggtgccg gcggtgcaca
     9901 gggcgatggt ctggcccttg ttctccgcca ccttcttgcc gttcaccagg atcgtgcagg
     9961 cgatctggtt gctgaccgtc gagttccggg tgtccgcggt gacggtgagc aggtagtcgc
    10021 ccgagttgaa ggacgccgtg ccgcgccatt cgtcggtgct ggccggggtc gtctcggtcc
    10081 gctggtcgtt gagcgagccg taccggatgg tcgcgccgcc ggaagcgctg acgacgaact
    10141 cgacggtgtt cttgtccccg gcgaccgccg ggacgtgcag ccccgactcg ccggtcgcga
    10201 agaaactggt gtagaccagt aacccgcccg cggccagcag tccggtgatc gaaagcgcga
    10261 gccccgcgat ccccattccc ttcttgtccg cttcaccctt cttgattttg gtcaaaccga
    10321 tcacggaaag aatcactccg atcacggcga gcggccaggc ggcgatcccg gcgaccggga
    10381 cgaaggcgac
//
