LOCUS       NZ_CP102415            25564 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Amycolatopsis roodepoortensis strain ZEL-1 chromosome, complete
            genome.
ACCESSION   NZ_CP102415
VERSION     NZ_CP102415.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis roodepoortensis
  ORGANISM  Amycolatopsis roodepoortensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Biodegradation of petroleum hydrocarbons by Amycolatopsis orientalis
            ZEL-1, genome analysis and its potential in plant protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) College of Life Sciences, Sichuan Normal
            University, No. 1819, Section 2, Chenglong Avenue, Longquan
            District, Chengdu 610101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024628825.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 06:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,064
            CDSs (total)                      :: 8,994
            Genes (coding)                    :: 8,928
            CDSs (with protein)               :: 8,928
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 44 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5897750
            Orig. end                         :: 5923314
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..25564
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="20"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..25564
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..25564
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:15564](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..15564
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    complement(136..154)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.07"
                     /tool="antismash"
     gene            283..1389
                     /locus_tag="NQK81_RS28125"
                     /old_locus_tag="NQK81_28125"
     CDS             283..1389
                     /NRPS_PKS="Domain: PKS_ER (13-362). E-value: 6.5e-14.
                     Score: 43.7. Matches aSDomain:
                     nrpspksdomains_NQK81_RS28125_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662084.1"
                     /locus_tag="NQK81_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28125"
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="WP_257483906.1"
                     /sec_met_domain="ADH_N (E-value: 6.9e-29, bitscore: 99.0,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.1e-18, bitscore:
                     66.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKAAVLWGLHEKWQVEEIDLDPPKAGEVLVKLTASGLCHSDEH
                     LVTGDLPFPYPLVGGHEGAGVVEAVGPDVTDIAEGDHVVMTFLPACGRCSYCAQGLGNL
                     CDTGAAVMLGPQLDNTYRFHARGEDVGQMCLLGTFSEYTVVPAASVVKIDDGIPLDKAA
                     LVGCGVTTGFGSAVRSGEVRAGDAVVVVGVGGIGINAIQGARIAGARLVVAIDPVEFKR
                     SKALEFGATHTAASMDEAWNTVSELTRGQLADVCVITTDVAEGAHILPALSLVGKRGRV
                     VVTAIGHPEETTITASLMELTLYEKQLRGSLFGSSNGQHDVPRLLELYNAGLLKLDELV
                     TTEYSLEDVNQGYQDMRDGKNIRGLIRF"
     aSDomain        322..1368
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS28125_PKS_ER.1"
                     /evalue="6.50E-14"
                     /label="NQK81_RS28125_PKS_ER.1"
                     /locus_tag="NQK81_RS28125"
                     /protein_end="362"
                     /protein_start="13"
                     /score="43.7"
                     /tool="antismash"
                     /translation="KWQVEEIDLDPPKAGEVLVKLTASGLCHSDEHLVTGDLPFPYPLV
                     GGHEGAGVVEAVGPDVTDIAEGDHVVMTFLPACGRCSYCAQGLGNLCDTGAAVMLGPQL
                     DNTYRFHARGEDVGQMCLLGTFSEYTVVPAASVVKIDDGIPLDKAALVGCGVTTGFGSA
                     VRSGEVRAGDAVVVVGVGGIGINAIQGARIAGARLVVAIDPVEFKRSKALEFGATHTAA
                     SMDEAWNTVSELTRGQLADVCVITTDVAEGAHILPALSLVGKRGRVVVTAIGHPEETTI
                     TASLMELTLYEKQLRGSLFGSSNGQHDVPRLLELYNAGLLKLDELVTTEYSLEDVNQGY
                     QDMRDGKNI"
     gene            1442..2425
                     /locus_tag="NQK81_RS28130"
                     /old_locus_tag="NQK81_28130"
     CDS             1442..2425
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662083.1"
                     /locus_tag="NQK81_RS28130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28130"
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="WP_257483907.1"
                     /transl_table=11
                     /translation="MAIDRNIEHGNRTGFGSLSEDGLRMESFPMRLFRKGNKKFWNPED
                     IDFSQDAKDFAAMNAGEKRLTSILAAQFMAGEESVTQDLQPFMKAMAAEGRLADEAYLT
                     QFTFEEAKHMQAFRLWFDAVGLGTDLHEFVEYSEPYTKIFTEELPKSLYALEKDPSAAA
                     QIRASVTYNHVVEGCLALTGYFAWAKVCKSRNILPGMQKVVKHIGDDERRHMAWGTFTC
                     RRHVAADDRNWDVVDERMQELLQPALQLIVRLFDVFEDDTPFGISIDEMTDYALDKVNR
                     RLESIESARGCPVEEVDEDYSPMQLEDKFAAEDEVAVAQALTAVGS"
     gene            2488..3510
                     /locus_tag="NQK81_RS28135"
                     /old_locus_tag="NQK81_28135"
     CDS             2488..3510
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662082.1"
                     /locus_tag="NQK81_RS28135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28135"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_257483908.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.5e-23, bitscore:
                     80.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQATSVQEPDTNVDLLFIGAGPVGLFGAYYAGFRGLSMAVVDAL
                     PEVGGQVQAIYPEKKIYDIAGLPGVQGQELIGNLVRQAAPFSPKYLLSQQVLRLERGEG
                     RWRATTTTGVVVEARAVVITSGVGAVTPRPIPAGEEFLGRGLCYFVPRLDVLDGKDVVV
                     VGGGDSAVDWALAALPRARSTTLVHRRRQFRAHEHSVQLLHKSACEVLVDANVTQLRGQ
                     DQLESVSIRVAGEPEVRETKAQIVVAALGFLMDLGPIKDWGLELEGRAIRVDSKMRTNL
                     PEVFAAGDAATFEGKVKLIAVGFGEVATAVNNAAVALDPRAELAPGHSSDLTVPARVGV
                     "
     gene            3552..3866
                     /gene="fdxA"
                     /locus_tag="NQK81_RS28140"
                     /old_locus_tag="NQK81_28140"
     CDS             3552..3866
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fdxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985025.1"
                     /locus_tag="NQK81_RS28140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28140"
                     /product="ferredoxin"
                     /protein_id="WP_257483909.1"
                     /transl_table=11
                     /translation="MAFVIGEPCVDVKDMTCIEECPVDCIYEGERMLYIHPSECIDCAA
                     CEPVCPVEAIKPAQQVDDRWRPFQESAKTAFESLGSPGGSTKVDPPIEDTEFVQKFEKN
                     "
     gene            3926..4552
                     /locus_tag="NQK81_RS28145"
                     /old_locus_tag="NQK81_28145"
     CDS             3926..4552
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662080.1"
                     /locus_tag="NQK81_RS28145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28145"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_257483910.1"
                     /transl_table=11
                     /translation="MTSMATIYCERAHENRVNDVFSVVASETDPWSLVRASQQGDRRAF
                     GLLYARFSMDVFRFARGRLRDEHAAEDVTSETFLRALRSISTVSYFGSSVRAWLMTIAR
                     NIILDHVKSGRFRHELTVSEFTEEEQAAVNPEFSIFLQPRRDEIARQMFALSEDQRVCL
                     RLRFFDGLSVSETAQVMKRKSSAVRALQHRAVRRLAELVDPVAVD"
     misc_feature    4412..4414
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4418..4420
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4611..6110
                     /locus_tag="NQK81_RS28150"
                     /old_locus_tag="NQK81_28150"
     CDS             4611..6110
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662079.1"
                     /locus_tag="NQK81_RS28150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28150"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_257483911.1"
                     /sec_met_domain="Aldedh (E-value: 7.7e-185, bitscore:
                     613.8, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMMSVEVPPEVRPFLDDASASIVIGATKTAAAEGGEIPTTDPAT
                     GTLLANIAAGGKADVDRAVDAARKAFAQWRRLSPAARGRVLLDVADAIDEHAEELAILE
                     TLDNGKPLTESTYLDVSLSAQIWRYFGGWTTKLGGQTLPVSPAVGEALVYTQREPLGVV
                     GAIVPWNFPLMIASWKLAPALAAGNTAVLKPSEFTSLSALRLTEVALEAGLPAGVLNVV
                     TGLGSDAGQALIDHPGVAKISFTGSTETGRRIVTASAGSLKKLTLELGGKSANIVFPDA
                     DIEAAAQGALNGIFLNQGQVCCAGSRLFVHRDIHDEVVAAIQAEAETIRLGHGLDEETQ
                     MGPLISARQLERVEGFVGAGIRDGAALVCGGRQPAGALAGGHFLEPTVFTDVRDEMSIA
                     TDEIFGPVLSVLKFDDEHEAVRRANASAYGLAAGVWTNDLKRAHRVARELEAGTVWVNM
                     YNMIDPAAPFGGYKSSGYGRDLGEESLLGYTQTKSVWIGLD"
     gene            6169..6576
                     /locus_tag="NQK81_RS28155"
                     /old_locus_tag="NQK81_28155"
     CDS             6169..6576
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662078.1"
                     /locus_tag="NQK81_RS28155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28155"
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="WP_257483912.1"
                     /transl_table=11
                     /translation="MGAFKDDTEVYKYIGGVFENGLADPDVGPKLKSSGVVLQITYTDP
                     DSVVTVDMPSGEVHTGQSSLTPHVELFMSADTGNRFWLGKVNLSVAMAKGQVRAKGPVS
                     KILKLVPAAKSLFPTYEAMLERDGRDDLLKA"
     gene            6626..8254
                     /locus_tag="NQK81_RS28160"
                     /old_locus_tag="NQK81_28160"
     CDS             6626..8254
                     /NRPS_PKS="Domain: AMP-binding (19-437). E-value: 3.6e-90.
                     Score: 294.4. Matches aSDomain:
                     nrpspksdomains_NQK81_RS28160_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662077.1"
                     /locus_tag="NQK81_RS28160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28160"
                     /product="fatty acid--CoA ligase"
                     /protein_id="WP_257483913.1"
                     /sec_met_domain="AMP-binding (E-value: 7e-100, bitscore:
                     333.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSTMQGTPLTVASILRHGVEIHGDRRVITATGEGFRQTTYEQVG
                     QRCGQLANALRRLGIAGDDRVATFQWNNQEHLEAYAAVPAMGAVLHTLNIRLSAEQIRF
                     IVGHAEDKVVIVDSSLAPILGRILPELDTVKVVLVTGDGALEPFEASGKAVLRYEEVLS
                     AESSAFAWIDTDESSGAAMCYTSGTTGDPKGVVYSHRSLYLHSMAACTGNGLGIAEQDR
                     ILPIVPMFHANAWGLPYAALMAGAELLMPDRFMQAAPLAEMVHSQRPTVAGAVPTIWND
                     VLLHTEKDTGLDFSSLRLVVCGGSAVPQALMEAFEDRLGVTILQAWGMTETSPMASVAR
                     PAVTASSDDSWRMRATQGRPVCGVEMRIVTEDGTPQPRDGRSVGELEVRGPWVTASYYE
                     VGDDARFHDGWLRTGDVGYISPDGFLTLTDRMKDVIKSGGEWISSVELESALAAHPDVF
                     EAAVIAVPDERWQERPLAAVVIREGATASPGELRTWLSGKLPRWWLPERWSFIEQIPRT
                     SVGKFDKKVLRGDYAGGRLKVETLD"
     aSDomain        6683..7936
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS28160_AMP-binding.1"
                     /evalue="3.60E-90"
                     /label="NQK81_RS28160_AMP-binding.1"
                     /locus_tag="NQK81_RS28160"
                     /protein_end="437"
                     /protein_start="19"
                     /score="294.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="VEIHGDRRVITATGEGFRQTTYEQVGQRCGQLANALRRLGIAGDD
                     RVATFQWNNQEHLEAYAAVPAMGAVLHTLNIRLSAEQIRFIVGHAEDKVVIVDSSLAPI
                     LGRILPELDTVKVVLVTGDGALEPFEASGKAVLRYEEVLSAESSAFAWIDTDESSGAAM
                     CYTSGTTGDPKGVVYSHRSLYLHSMAACTGNGLGIAEQDRILPIVPMFHANAWGLPYAA
                     LMAGAELLMPDRFMQAAPLAEMVHSQRPTVAGAVPTIWNDVLLHTEKDTGLDFSSLRLV
                     VCGGSAVPQALMEAFEDRLGVTILQAWGMTETSPMASVARPAVTASSDDSWRMRATQGR
                     PVCGVEMRIVTEDGTPQPRDGRSVGELEVRGPWVTASYYEVGDDARFHDGWLRTGDVGY
                     ISPDGFLTLTDRMKDVIKS"
     CDS_motif       7166..7222
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NQK81_RS28160_0001"
                     /evalue="4.60E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="NQK81_RS28160"
                     /protein_end="199"
                     /protein_start="180"
                     /score="22.3"
                     /tool="antismash"
                     /translation="AMCYTSGTTGDPKGVVYSH"
     misc_feature    8120..8122
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8306..9247
                     /locus_tag="NQK81_RS28165"
                     /old_locus_tag="NQK81_28165"
     CDS             8306..9247
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="NQK81_RS28165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28165"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_257483914.1"
                     /transl_table=11
                     /translation="MDDSKSASKLEFDGVTSVLGEEPVLHRLRLSVPAGQITVILGQPG
                     SGKTTLLKHLVGILPPTGGSIRLDGRDVWSMTSDELASARGGIAAVIGGTHLDGSRLFS
                     SMSVYENVACVARHAGAADDEVHAAVAPLIQEFGLEVFADSFPEEIPAHARRRVLLAQV
                     LVSGMPLIILDDVDTTCDPRYLDAVVRAIKSVNEKRRATVLITTNDLSFARGVADEVAV
                     LWNGKIASLGPPDVLLRGVRTGDEFGRRFFRSDLAGPLQVDVGGGDLHGDERARRFFSV
                     DPGRPVWCVAALLLLVVVFVFWSSAGTIFGRP"
     gene            9262..9567
                     /locus_tag="NQK81_RS28170"
                     /old_locus_tag="NQK81_28170"
     CDS             9262..9567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875183.1"
                     /locus_tag="NQK81_RS28170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28170"
                     /product="hypothetical protein"
                     /protein_id="WP_257483915.1"
                     /transl_table=11
                     /translation="MADPYLDSVYTSELAALAHAIEIVDDHADLNSRYRGLIDDSRVSL
                     GSGRLRLTQARGLAKRLVVLARAAGTDMRAQLTKTERDALDAGLAQAAVLIRIPPP"
     gene            9904..10011
                     /gene="mftA"
                     /locus_tag="NQK81_RS28175"
                     /old_locus_tag="NQK81_28175"
     CDS             9904..10011
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658597.1"
                     /locus_tag="NQK81_RS28175"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis.;
                     Mycofactocin is a small molecule electron carrier derived
                     from the final two amino acids, Val-Tyr, of MftA, the
                     mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28175"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_257483916.1"
                     /transl_table=11
                     /translation="MTEKIAEQATPAEDTVVAEDLLVEEVSIDGMCGVY"
     gene            10001..10288
                     /gene="mftB"
                     /locus_tag="NQK81_RS28180"
                     /old_locus_tag="NQK81_28180"
     CDS             10001..10288
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806422.1"
                     /locus_tag="NQK81_RS28180"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA.; MftB, a small
                     protein, is a peptide chaperone that assists the radical
                     SAM enzyme MftC in performing two modifications to the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     peptide, MftA. MftB's role is analogous to the role of PqqD
                     in the biosynthesis of PQQ, a cofactor that derives
                     entirely from a Tyr and a Glu in the precursor PqqA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28180"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_257483917.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 6.7e-29,
                     bitscore: 99.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTEGRAFDLDAGWRLDDRVAVRPERFGALLYHFGTRRLSFLKSP
                     ALLDVVRTLADHPTARAACAGAGVGPPELPRYRAALAELAVSRMIQPRSV"
     aSDomain        10031..10270
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_NQK81_RS28180_Mycofactocin_RRE.1"
                     /evalue="2.10E-31"
                     /identifier="RREFam016.1"
                     /label="NQK81_RS28180"
                     /locus_tag="NQK81_RS28180"
                     /protein_end="90"
                     /protein_start="10"
                     /score="99.0"
                     /tool="antismash"
                     /translation="DAGWRLDDRVAVRPERFGALLYHFGTRRLSFLKSPALLDVVRTLA
                     DHPTARAACAGAGVGPPELPRYRAALAELAVSRMI"
     gene            10288..11469
                     /gene="mftC"
                     /locus_tag="NQK81_RS28185"
                     /old_locus_tag="NQK81_28185"
     CDS             10288..11469
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076819.1"
                     /locus_tag="NQK81_RS28185"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="NQK81_28185"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_257483918.1"
                     /sec_met_domain="PF04055 (E-value: 1e-24, bitscore: 86.8,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.7e-08, bitscore: 33.2,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.3e-13, bitscore:
                     50.1, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLVDEFQYGLDAPICLTWELTYACNLSCVHCLSSSGRRDPRELS
                     TAECKALIDEFERMQVFYVNIGGGEPTVRPDFWELVDYATEHHVGVKFSTNGIKITPEV
                     ARRLAASDYVDVQISLDGATAEVNDHVRGPGSYATAIRAMENLAAAGFENFKISVVVTR
                     HNAGQLDEFKAIADRYRAQLRITRLRPSGRGADVWDELHPTAAQQRELYDWLVARGENV
                     LTGDSFFHLAGYGDGALPGLNLCGAGRVVCLVDPVGDVYACPFAIHDTFLAGNVRDGGG
                     FTRVWRESELFTELRRPQSGGACTSCSAFDSCRGGCMAAKFFTGLPLDGPDPECVRGHG
                     ERALAGVVAGSVPKPSLDHSHRRRPVPITIGMRRPPERACDENPLAGFAPPGR"
     gene            11473..12153
                     /gene="mftE"
                     /locus_tag="NQK81_RS28190"
                     /old_locus_tag="NQK81_28190"
     CDS             11473..12153
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152862.1"
                     /locus_tag="NQK81_RS28190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28190"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_257483919.1"
                     /transl_table=11
                     /translation="MRLADLSWPDVAERAASGAILAVPVGATEQHGPHLPLTTDTDIAL
                     ALCDRLAGTRTDVIVAPAVAYGSSGEHARFAGTLSIGQEAIELLLLELGRSAAETFHRL
                     LFVSAHGGNAGPVARAVTRLRAESRDVEVFQPRWSGEPHAGRPETALQLALRPEAVRME
                     RAVPGDRRPIGELMPHLLGGGVWAVTATGVLGDPTGATAVEGAVLLDRLTDQLVSHVDG
                     WGGR"
     gene            12150..12935
                     /locus_tag="NQK81_RS28195"
                     /old_locus_tag="NQK81_28195"
     CDS             12150..12935
                     /NRPS_PKS="Domain: PKS_KR (4-178). E-value: 6.8e-14. Score:
                     44.1. Matches aSDomain:
                     nrpspksdomains_NQK81_RS28195_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658593.1"
                     /locus_tag="NQK81_RS28195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28195"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_257483920.1"
                     /transl_table=11
                     /translation="MTSRTALVTGAARGIGAATVRILARQGYAVLAVDLAADDPTLPYP
                     MGSKAELAAVVEQAGGNVEAFVADVRNFSAVAAAVAEAERRWGGLDVAVAAAGVVAGGV
                     PLWEVPPEQERAVLEVDLNGVVHLARAAVPALLKRPRPRSGRFLAVASAAATRGLPLLA
                     AYCAAKAGVGGLIRALGAELGESGVTANAVSPGSTDTPILAESARLYDLPAAVAFAAQQ
                     PVHRLLDPDEVAAVLAFLAGPDSGAMTGAMVPVDGGLAL"
     aSDomain        12162..12683
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS28195_PKS_KR.1"
                     /evalue="6.80E-14"
                     /label="NQK81_RS28195_PKS_KR.1"
                     /locus_tag="NQK81_RS28195"
                     /protein_end="178"
                     /protein_start="4"
                     /score="44.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGAARGIGAATVRILARQGYAVLAVDLAADDPTLPYPMGSK
                     AELAAVVEQAGGNVEAFVADVRNFSAVAAAVAEAERRWGGLDVAVAAAGVVAGGVPLWE
                     VPPEQERAVLEVDLNGVVHLARAAVPALLKRPRPRSGRFLAVASAAATRGLPLLAAYCA
                     AKAGVGGLIRA"
     CDS_motif       12168..12230
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NQK81_RS28195_0001"
                     /evalue="4.00E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="NQK81_RS28195"
                     /protein_end="27"
                     /protein_start="6"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LVTGAARGIGAATVRILARQG"
     gene            12932..14365
                     /gene="mftF"
                     /locus_tag="NQK81_RS28200"
                     /old_locus_tag="NQK81_28200"
     CDS             12932..14365
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658592.1"
                     /locus_tag="NQK81_RS28200"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969).; Members of this protein family, MftF,
                     are glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28200"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_257483921.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.8e-20,
                     bitscore: 71.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIPLPPGFRIDLDPDVRKLADGLWFGGSPARVLRLTAAGQAAWR
                     VLDAGPVTTAATGVLARRLTDAGLAHPVPPAQDGPPGVTIVVPVRDRAELLARCLAGLG
                     SRHPVLVVDDGSADPAAIAAVAAAHGAKLVCRDVNGGPGAARNTGLEQVSTELVAFVDS
                     DCVPPEDWACLLAAHFADPLLAAIAPRVRALAQDTWSGRYTRAAGCLDLGDRPARVAPG
                     TRMSYVPTAMLVARRSALAAVADGGQVFDPRLRVGEDVDLVWRLHAAGFRVRYAPDVQV
                     GHHEPASWRGLLGRRVRYGVSAAPLALRHPKALAPLVLHFWPALTVGALLARRPLLAAV
                     AFGGSVRSTVRLLRANDVPSDGVVRALATAVHQTWLGCGRHGTQFAAPLLVALLVPGGR
                     RRWGRRAAVASLLLGPPLTAWASKRPKLDPIRYTAAAIADDIAYGAGVWAGCLTSRTAA
                     PVRPAFLRRPLRFDPKGKR"
     gene            14362..15564
                     /gene="mftD"
                     /locus_tag="NQK81_RS28205"
                     /old_locus_tag="NQK81_28205"
     CDS             14362..15564
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658591.1"
                     /locus_tag="NQK81_RS28205"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="NQK81_28205"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_257483922.1"
                     /sec_met_domain="TIGR03966 (E-value: 4.5e-210, bitscore:
                     696.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTWFESVAEAQRRAKKRLPGSVYSALIAGAEKGLTLSDNLGAFD
                     ELRFAPRTAGLSGDRDLSTHVLDRDVALPVLISPTGVQAVHPDGEVAVARAAAARGTSM
                     GLSSFGSKPIEEVVAANPNTYFQIYWTGTRHDILRRLDRARTAGAVGIILTLDWSFSHS
                     RDWGSPHIPERLTFRELLRFAPEGLTRPGWLLDYAKTRRLPDLSVPNMVAPDEQAPTFF
                     GAYGQWMQTPPPSWEDVRWLRAQWNGPFLLKGIIRADEARRAVDSGVSAISVSNHGGNN
                     LDGTPATIRALPSIADAVGDQVEVLLDGGIRRGSDVVKALALGARAVLIGRAYLWGLAA
                     NGQPGVENVLDVLRNGIDSTLLALGKKSVRDLTREDVLVPEGFETELGATSAADRSGLS
                     G"
     gene            <15558..>15868
                     /locus_tag="NQK81_RS28210"
                     /old_locus_tag="NQK81_28210"
                     /pseudo=""
     CDS             15558..>15868
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157567.1"
                     /locus_tag="NQK81_RS28210"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="NQK81_28210"
                     /product="MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLGPKKMISGGLWALAAGLVWLSLVSPTGRFRRRRPARVAGRGTW
                     SGAGVQPVAGHRDLECEAGDRRARVGHREHLVPDRVGARSGRDDRGRRVLSARGGSEG"
     misc_feature    15561..15563
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            16028..16294
                     /locus_tag="NQK81_RS28215"
                     /old_locus_tag="NQK81_28215"
     CDS             16028..16294
                     /GO_function="GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /locus_tag="NQK81_RS28215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28215"
                     /product="STAS domain-containing protein"
                     /protein_id="WP_257489869.1"
                     /transl_table=11
                     /translation="MSITVTGEIDLVTTEFLKGEFSQALDPTPTSVVVDLNAVDFCDTT
                     GLSVLVGLNNRCVADHIALRVLPSARIHRLLLRTGLSNLLPIR"
     gene            complement(16491..17276)
                     /locus_tag="NQK81_RS28220"
                     /old_locus_tag="NQK81_28220"
     CDS             complement(16491..17276)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422870.1"
                     /locus_tag="NQK81_RS28220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28220"
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="WP_257483923.1"
                     /transl_table=11
                     /translation="MLIAAALAGLLIGLALGLLGAGGSILAVPALVYAAGQPLAAAIPA
                     SLLIVGVSSAAAVIPRLRTRAVRWPIALVFGAAGIPAAFGGAALGRSLPPRVLLLGFAA
                     LMAVVAVRMLTEHRSSGGACRTSGGRINWRACLPKALAAGAVVGVLTGLFGVGGGFLIV
                     PALTLLLGLDAAQAVATSLVIIVINSAAGLAARADAPIDWTTTGIFGGAAVLAALAAGR
                     LATRLPERAVRRGFAYLVLLIAAAIAVTAIVDPATLPTR"
     gene            complement(17280..18662)
                     /locus_tag="NQK81_RS28225"
                     /old_locus_tag="NQK81_28225"
     CDS             complement(17280..18662)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982581.1"
                     /locus_tag="NQK81_RS28225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28225"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_257483924.1"
                     /transl_table=11
                     /translation="MLDVEVIETSSLGDRSYLAHDDEVAVVVDPQRDIDRVLALAGRLD
                     VRIAWVLETHVHNDYVSGGLQLARLTGARYGIAADDEVPFDRVPLHEGDVLNVSERMHI
                     RPVATPGHTFHHLSYILDGPGGPEGVFTGGSLLLGTTGRTDLLGQDHAHDLARQQRSSV
                     HRLAQLLPDGAQVWPTHGFGSFCSATQASGDASTIGRERQINPALTLAETEFVEQTLAG
                     LDDYPAYYAHMGVRNTAGPEPIDLTPVATADPVELLRRVDAGEWVVDLRSRKAFVRSHL
                     AGTVSLGLDGPMVTWLGWLIDWGAPITLLGDTPDQVAQAQRELVRIGIDRPAAAATGDP
                     AQWAGDHAERLAALATASFGDLAAALAHRGPDGLPAPAVVLDVRLPHEWRESHVRDAVH
                     IPLPELPQRLGDLPAGPVWVHCGSGYRAAAAASLLARAGRTAVLIDDAFGHAETAGVPM
                     AA"
     gene            complement(<18909..19010)
                     /locus_tag="NQK81_RS45455"
                     /pseudo=""
     CDS             complement(<18909..19010)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012790.5"
                     /locus_tag="NQK81_RS45455"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAVHEAAVGDVDRALSVGARLVDVRSPGEYARAH"
     gene            complement(19086..19355)
                     /locus_tag="NQK81_RS28230"
                     /old_locus_tag="NQK81_28230"
     CDS             complement(19086..19355)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943207.1"
                     /locus_tag="NQK81_RS28230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28230"
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="WP_257483925.1"
                     /transl_table=11
                     /translation="MEMTPELVGDALVRLRRAQGQLAAVIGMIEAGEDCATVLTQLAAV
                     SRALDRAGFKVVASGMRYCQAARDRGEEPSMTEAELEKLFLALA"
     gene            complement(19964..20802)
                     /locus_tag="NQK81_RS28235"
                     /old_locus_tag="NQK81_28235"
                     /pseudo=""
     CDS             complement(19964..20802)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612292.1"
                     /locus_tag="NQK81_RS28235"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28235"
                     /product="IS982 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTKELNTLLVALYVLIDDHVVPPRAGRGRRPELTDSELITMAVAQ
                     VLQRYDSERRWIRRIHADSEWRSMFPAMLGQSGYHKRLKTAQPLLCKAILTVAACCPSW
                     FDDLWITDATPAPCGMSRETVKRSDLAGHAGYGYCASHTRYYWGLKLHLVCAGDGMPIM
                     WCLANPKLGERQVPAALLNHNHHLIREGQVLLAIKVSPVRTSNS"
     misc_feature    20849..20864
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.95"
                     /tool="antismash"
     misc_feature    complement(20927..20940)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.25"
                     /tool="antismash"
     gene            complement(20956..21174)
                     /locus_tag="NQK81_RS28240"
                     /old_locus_tag="NQK81_28240"
     CDS             complement(20956..21174)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NQK81_RS28240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NQK81_28240"
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="WP_257483926.1"
                     /transl_table=11
                     /translation="MVVPTRTGHRFVADQLFGGRFGAIIVADVRVQFADFPGRIVYHCH
                     IFDDEDAGMMATVQVLLTTSPGVDRHW"
     gene            complement(21471..21863)
                     /locus_tag="NQK81_RS28245"
                     /old_locus_tag="NQK81_28245"
     CDS             complement(21471..21863)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658985.1"
                     /locus_tag="NQK81_RS28245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28245"
                     /product="DUF302 domain-containing protein"
                     /protein_id="WP_257483927.1"
                     /transl_table=11
                     /translation="MDYGIAVRSAAPMAETVERVRTALADQGFGVLTEIDVQATLKAKL
                     GQESEPYLILGACNPQLAHQALGVDRRIGLLLPCNVVVRVDGESTMVEALDPQVIVSVP
                     EDSRLQPIADEAGRRLRAALQAVGER"
     gene            complement(<21963..22178)
                     /locus_tag="NQK81_RS28250"
                     /old_locus_tag="NQK81_28250"
                     /pseudo=""
     CDS             complement(<21963..22178)
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440779.1"
                     /locus_tag="NQK81_RS28250"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28250"
                     /product="cation diffusion facilitator family transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAAEGSTRAVVTAMFANLGIAVTKFVAFLLTASSSMLAESVHSLA
                     DTSNQALLLLGGKRAKRAATPEHPFGY"
     gene            complement(22449..22946)
                     /locus_tag="NQK81_RS28255"
                     /old_locus_tag="NQK81_28255"
     CDS             complement(22449..22946)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NQK81_RS28255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NQK81_28255"
                     /product="hypothetical protein"
                     /protein_id="WP_257483928.1"
                     /transl_table=11
                     /translation="MTTRRAIAAAVLVATLGAACSTPATEPQSARQSEVAEKGRSVMPF
                     DLEKTTHRFTPRPDGLLQEVVADVPGDMAQIGPIRTHLTEESTRFRSGDFTDPASIHGG
                     TMPGLTELTAGAPRITIDYAELPDGASLSFHTNDPALITALHAWGEAQVSDHGEHAEHG
                     NR"
     gene            complement(22933..23271)
                     /locus_tag="NQK81_RS28260"
                     /old_locus_tag="NQK81_28260"
     CDS             complement(22933..23271)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024854.5"
                     /locus_tag="NQK81_RS28260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28260"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_257483929.1"
                     /transl_table=11
                     /translation="MSVAEAHALTILRDGPLHQGELGSRLNLGKSTTSRLTDGLVDRGW
                     VLRRPDPADGRARLLSLTDSGTEVSAGVVERRGKRLAQLLSNIDPEQHTNVINALRLLK
                     EATTSDHP"
     gene            complement(23471..24646)
                     /locus_tag="NQK81_RS28265"
                     /old_locus_tag="NQK81_28265"
     CDS             complement(23471..24646)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240656.1"
                     /locus_tag="NQK81_RS28265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28265"
                     /product="cytochrome P450"
                     /protein_id="WP_257483930.1"
                     /sec_met_domain="p450 (E-value: 1.4e-19, bitscore: 69.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASARLPFDQPDLLRVAPQLCALQAQGPIHRIRTAVGDEAWLVT
                     DHAQVRRLLSDDRLGRSHREPENAARTGESALFGGPHGDFDTESTHHARMRTLLQPHFS
                     PKHMRALRPRIDTLTTQLLDDLDGGPQPADLHTALALPLPILVICELLGVPYEDRDQFR
                     AWTEAAGDIRDRARSEQGLGELFGYGMRLVVDKRKSPGDDVISRLCATDGVSDSEAAML
                     SMALLFAGHETTVVQIGMGALLLLANPGQWNALLDNPGLVPNAIEELLRAPGKSGGGIP
                     RYARTDFEIDGVTIQAGELVLLDNGAANHDPAVFAEPDHVDVTRHASAHLTFGHGLRYC
                     IGAPLARMELQAVFTQLIPRFPTMRLAVPVEDLTIRRDVLTGGLTALPVTW"
     misc_feature    complement(24224..24226)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    24664..24679
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.33"
                     /tool="antismash"
     misc_feature    complement(24714..24729)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            24733..25350
                     /locus_tag="NQK81_RS28270"
                     /old_locus_tag="NQK81_28270"
     CDS             24733..25350
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693425.1"
                     /locus_tag="NQK81_RS28270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_28270"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_257483931.1"
                     /transl_table=11
                     /translation="MAEPEPVRRMPRAERREQILDAATRAFARTGYAATGLDDVAAEAG
                     ITRVLLYRHFDSKGDMYRAVLDRACARLGTAVGSEGDYDEGSLPALMRSAADDPDGFRL
                     LFRHAVREPEFRDVVDGIRSASAEVTRKELAAMIPDGPWLDWAVQLVPAVTLEAVIAWL
                     DAGQPDPDQAAGRIAQIVHGIVDVAGAVDRPKGYRDERRTRQ"
     misc_feature    complement(25441..25455)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 23.2"
                     /tool="antismash"
ORIGIN
        1 gatggccgga tcagcttccg gccgcgctcg ggcggtggca tggtcgtgtg cctcgaaatg
       61 ccggtcgact gagcatttcg cggtgttccg gtcgaccccg gcccatccga tgacctccct
      121 ttaggttctg gctctgctgc gtgtcggtgg cattcgcgag gggtccatgc gggcccgcac
      181 agttggcacg tgcgggccat caatggctcg cgcgaccggt gctaatcgag ccaggtcttg
      241 tgatgatgac ccgagacctt caccgttaag gagttgacgt caatgaagac gaaggctgcg
      301 gtcctttggg gactgcacga gaagtggcag gtcgaggaaa tcgacctgga cccgccgaag
      361 gcgggcgagg tcttggtcaa gctgaccgcg agcgggctgt gccactccga cgagcatctc
      421 gtcaccggag atctgccctt cccctacccg ctggtcggcg gacatgaagg cgccggcgtg
      481 gtcgaagccg tcggccccga cgtcacagac atcgccgagg gcgaccacgt ggtcatgact
      541 ttcctacctg cctgcggcag gtgctcgtac tgtgcacagg gcctgggcaa tctgtgcgat
      601 accggcgccg cggtgatgct cggcccgcag ttggacaaca cctaccgctt tcacgcacgc
      661 ggcgaggatg tcggtcagat gtgcctgctc ggaacgttct ccgagtacac ggtcgtaccg
      721 gcggcgtcgg tcgtaaagat cgatgatggt attcccctgg acaaggccgc gctcgtcggt
      781 tgtggcgtaa ccaccggctt cggcagcgct gttcgttccg gcgaagtgcg agccggggac
      841 gcggtcgtcg ttgtcggcgt cggcggcatc ggcatcaatg cgatccaggg cgcccggatc
      901 gcaggcgccc gcctcgtcgt cgcgatcgac cccgtcgaat tcaagcgatc gaaagccctc
      961 gagttcggtg ccacccacac agccgcgtcg atggacgagg cgtggaacac cgtcagcgag
     1021 ttgacccgcg gtcagctggc cgacgtctgc gtgatcacca ccgatgtcgc cgaaggggct
     1081 cacatcctgc ccgctctttc gctcgtcggc aaacgcggca gggttgtcgt cacagccatc
     1141 ggccaccccg aagaaacgac gatcacggcc agcctgatgg aactgacgct gtacgagaag
     1201 cagctccgag gttcgctgtt cggttcgtcc aacggccagc acgacgtacc gcggctgctc
     1261 gagctctaca acgccgggct gctgaagctc gatgagcttg tcaccaccga gtactccctg
     1321 gaggacgtca accagggcta tcaggacatg cgcgacggca agaacattcg cggactcatt
     1381 cgcttctagt tcagctcggt cgcccgccac agctcaaatt cgtgatgtga ggacagtttt
     1441 catggcaatc gatcgcaaca tcgagcacgg caaccgcacg ggcttcggca gcctgagcga
     1501 ggatggcctc cgcatggagt cgttcccgat gcgcttgttc cgcaagggaa acaagaaatt
     1561 ctggaacccc gaagacatcg acttttcgca ggacgccaag gacttcgccg cgatgaacgc
     1621 cggggagaaa cggctcacgt cgattctggc cgcgcagttc atggccggcg aggaatccgt
     1681 gacgcaggac ctgcagccgt tcatgaaggc gatggccgca gagggcaggc tcgccgacga
     1741 ggcatacctg acgcagttca ccttcgaaga agccaaacac atgcaggcct tccgcctgtg
     1801 gttcgacgca gtcggcctcg ggactgacct tcacgagttc gtcgagtaca gcgagccgta
     1861 cacgaagatc ttcaccgagg agctgccgaa atctctgtac gcgctggaga aggacccttc
     1921 ggcggccgcg cagatccgcg cgtctgtgac ctacaaccac gtcgtcgaag gctgcctcgc
     1981 gctcaccggc tacttcgcct gggcaaaagt gtgtaagagc cgcaacatcc tgcccggcat
     2041 gcagaaggtc gtgaagcaca tcggtgacga cgagcggcgt cacatggcgt ggggcacctt
     2101 cacgtgccga cggcacgtcg ccgccgacga ccgcaactgg gacgtcgtcg acgagcggat
     2161 gcaggaactg ctccagccgg cgctccagct gatcgtgcgg ctgttcgacg tctttgagga
     2221 cgacacgccg ttcggtatca gcatcgacga gatgaccgac tacgcgctgg acaaggtcaa
     2281 ccgcaggctc gagtcgatcg agagcgcgcg cggctgcccg gtggaggagg tcgatgagga
     2341 ctactccccg atgcagctcg aggacaagtt cgccgcggaa gacgaagtgg cggtcgcgca
     2401 ggcgctcacg gccgtcgggt cctgatacgc cgaccgacct cactcgacac caggtcgacg
     2461 ccgacctcga acccaaggac catcgacatg tcgcaggcca cctctgtgca agaaccggac
     2521 acgaacgtcg acctgctctt catcggcgcc ggaccagtgg gtctctttgg cgcgtactac
     2581 gcaggatttc ggggcctgag catggcggtg gttgacgccc ttcccgaggt cggcggccag
     2641 gtgcaggcga tctacccaga gaagaagatc tacgacatcg cgggcctgcc gggagtgcag
     2701 ggccaagagc tcatcggcaa tctcgttcgg caggccgcgc cgttttcccc gaagtacctg
     2761 ctgtcgcaac aagtgctgcg actcgaacgc ggtgaaggtc ggtggcgagc cacgactacc
     2821 accggcgtgg tcgtcgaagc ccgggctgtc gtgatcacct ccggtgtcgg agccgtcacc
     2881 ccgcggccca tcccggcagg cgaggagttc cttggccgtg ggctgtgcta cttcgtaccg
     2941 cggctggacg tgctggacgg caaggacgtc gtcgtggtcg gaggtggcga ttccgcggtc
     3001 gactgggcct tggctgcgct tcccagggcg cggtcgacca cgctcgtgca ccgccgtcgg
     3061 cagttccgcg cccacgagca ctcggtccag ctgctgcaca agtcggcctg cgaggtactg
     3121 gtcgacgcca atgtgactca actgcgaggg caggaccagt tggagagcgt cagcatccgg
     3181 gtggcaggcg aacccgaggt tcgtgagacc aaggcgcaga ttgtcgtcgc cgcgctcggc
     3241 tttctgatgg atctcggccc gatcaaggac tggggcctcg aactcgaggg gcgggcgatc
     3301 cgggtcgaca gcaaaatgcg gacgaacctg ccggaggtct tcgcggccgg cgacgcggcc
     3361 actttcgagg gcaaggtcaa gctcatcgct gtcggcttcg gtgaagtggc gaccgctgtg
     3421 aacaacgccg ctgtcgcgct cgacccgcgg gcggagctcg cccccgggca ctcgagcgac
     3481 ttgaccgtcc cggcgcgggt cggcgtctga gcccagcgac attcatcgaa taatcctgag
     3541 gaggtggact gatggctttc gtcatcggag aaccgtgcgt ggatgtgaag gacatgacct
     3601 gcatcgagga atgcccggtc gattgcatct acgagggcga gcgcatgctc tacattcacc
     3661 cttccgagtg catcgattgc gcggcgtgtg agccggtctg cccggtcgaa gccatcaagc
     3721 cggcgcagca ggtcgacgac cgctggcgac cgttccaaga gtcggcgaag acggccttcg
     3781 agtccctcgg tagcccgggg ggttccacga aggtcgaccc gccgatcgag gatacggaat
     3841 tcgtgcagaa atttgagaag aactagccgc tatacggcac gcatcggccc gatgccggcg
     3901 gagagcgggt cataaggagt tccggatgac gtcaatggcg acgatttact gcgaacgtgc
     3961 gcacgagaac agagtgaacg acgtgttctc tgttgtggct tccgaaactg atccatggtc
     4021 cttggtccgc gcctcgcaac agggagatcg gcgagcattc ggcctgctgt atgcgagatt
     4081 ctcaatggac gtgttccggt ttgcgcgagg aaggcttcga gacgagcatg ccgccgagga
     4141 cgtcaccagt gaaaccttcc ttcgcgctct ccgtagtatc agcacggtga gttacttcgg
     4201 gtccagcgtt cgggcttggc tgatgaccat agccagaaac ataatcctgg atcacgtcaa
     4261 gtcggggcgg ttccggcatg aattgacggt ttcggagttc actgaagaag aacaagccgc
     4321 cgtgaatccg gaattcagca tctttttgca gcctcgccgg gacgagatag ctcggcagat
     4381 gttcgcgttg agtgaagacc agcgggtctg tttacggtta aggttcttcg acgggctgtc
     4441 cgtatcggaa acggcgcagg tgatgaagcg aaagagcagt gcggtgcgcg cgttgcagca
     4501 ccgcgccgtc cggaggctgg cggagttggt cgacccggtc gcggtcgatt gagagccgcg
     4561 gtgggaacaa gattgcttca tgaagcattg agtcggaaag gcgtttgctg atgacgatga
     4621 tgtcggtcga ggttccgccg gaggtacgtc ccttcctgga tgatgcgtcc gcgagcatcg
     4681 tcatcggtgc gacgaagacg gcggcggccg agggagggga gatcccgacc acggatcccg
     4741 cgacgggcac gctgctggcg aatatcgcgg ccggtggcaa ggcggacgtg gaccgcgccg
     4801 tcgatgccgc acgaaaggca ttcgcacaat ggcggcgcct cagcccggcc gcgcgtggac
     4861 gcgtgctgct cgacgtcgcc gatgcgatcg acgagcacgc cgaggagttg gccatcctcg
     4921 agacactgga caatggcaaa ccgctcaccg agtcgactta cctcgatgtc agcctctccg
     4981 cacagatctg gcggtatttc ggtggatgga ccacgaagct cggcggtcag accctgccgg
     5041 tctccccggc cgttggggag gcactggtct atacccagcg tgaaccactt ggagtggtcg
     5101 gcgcgatcgt gccgtggaac ttcccgctga tgatcgcgtc gtggaagttg gcgcccgcgc
     5161 tcgccgcagg aaatacggca gtgttgaagc cctctgagtt cacttcgctg agcgccctgc
     5221 gcctgactga ggtcgcgctc gaggcggggc ttcctgctgg tgtgctcaac gtggtcacgg
     5281 gcctggggtc ggacgccggc caggcgctta tcgaccaccc cggcgtcgcg aagatctcgt
     5341 tcaccgggtc gaccgagacc ggacggcgga tcgtgaccgc tagcgctgga agtctgaaga
     5401 agctcacgct cgaactgggc gggaagtccg cgaacatcgt cttcccggac gccgacatcg
     5461 aggcggctgc gcaaggtgct ctcaacggca ttttcctgaa ccaaggccag gtgtgctgtg
     5521 cggggtcgcg gctgttcgtc caccgcgaca ttcacgacga ggtagtggcc gcaattcagg
     5581 cggaggccga gacgatccgg ctaggccacg gcttggacga agagactcag atggggccct
     5641 tgatctcggc gcggcaactg gaacgggtgg aaggcttcgt cggagccggc atccgcgacg
     5701 gtgcggcgct ggtatgcggt ggccgacagc ctgcaggagc gttggccggc gggcattttc
     5761 tcgagccgac ggtgttcaca gacgtgcggg acgagatgtc catcgccacc gatgaaatct
     5821 tcggtccggt gctttcggtc ctgaagttcg acgatgagca cgaggcagtg cgtcgtgcca
     5881 acgccagcgc ctacggcctc gccgcgggcg tgtggaccaa cgacctgaag cgagctcacc
     5941 gggtcgcgcg cgagctggaa gcaggcacgg tctgggtgaa catgtacaac atgatcgacc
     6001 cggcggctcc cttcggcggg tacaagtcct cgggttatgg acgtgacctc ggggaggagt
     6061 cgctactggg gtatacgcag acgaagtccg tttggatcgg gctggactga accccgccaa
     6121 ctctcaccat tccacagatt ccgtaagaag ctcaacgagg agtacaaaat gggtgcattc
     6181 aaggacgaca ccgaggttta caagtacatt ggcggtgtct tcgagaacgg tctggcagac
     6241 ccggacgtcg ggccgaagct gaagagctcc ggcgtcgtgc tccagatcac ctacaccgac
     6301 cccgactcgg tcgtcacggt cgacatgccc tctggcgagg tccacaccgg ccagagcagc
     6361 ctgacgccgc atgtcgaact gttcatgtcc gcggacaccg gtaaccggtt ctggctcggc
     6421 aaggtcaacc tgtccgtcgc gatggcgaag ggccaggtgc gggcgaaggg cccggtctcg
     6481 aagatcttga agctcgtgcc cgcagcgaag tctctcttcc cgacctatga agcgatgctg
     6541 gagcgcgacg ggcgcgacga cctcctgaag gcgtgagtgg cgtgcgaagt tccgacgacg
     6601 acgtcggcgt gacggaggtc aagtaatgcg tagcaccatg cagggcacgc cgctcacggt
     6661 cgcgtccatc ctgcggcacg gcgtggagat tcatggcgac cgaagggtga tcaccgcgac
     6721 aggtgaggga ttccgccaga cgacctacga acaggtgggc caacggtgcg gacagctcgc
     6781 aaacgcgctg cgccgactcg ggatcgccgg cgacgaccgg gtggccacct ttcaatggaa
     6841 caaccaggaa cacctggagg cctacgccgc ggttccggcg atgggcgccg tgctgcatac
     6901 gctcaatatc cggttgtccg cagaacagat tcgcttcatc gtcggtcacg ccgaagacaa
     6961 ggtcgtcatc gtcgactcgt cgctcgcgcc aatactgggc cgaatacttc ccgaactcga
     7021 tacggtcaag gtcgtgctgg tcactggaga cggtgccctg gaacccttcg aggccagcgg
     7081 gaaggcagtg cttcggtacg aggaggttct cagcgccgag tcgtcggcat tcgcctggat
     7141 cgacacggac gagagttccg gtgccgcgat gtgctacacc agcggtacca cgggcgatcc
     7201 gaaaggtgtg gtgtacagcc accgttcgct gtacctgcac tcgatggccg cctgcacggg
     7261 caacggcctg ggcatcgccg agcaggaccg gatcctgccg atcgtgccga tgttccacgc
     7321 caacgcctgg ggactaccgt atgccgcctt gatggccggt gcggagctgt tgatgcccga
     7381 ccggttcatg caggcggctc cgctcgccga gatggtccac tcccagcggc ccaccgtcgc
     7441 aggggcggtc ccgacgatct ggaacgacgt cctgctgcac acggagaagg acactggcct
     7501 ggatttctcg tcgttgaggc tggtggtctg cggcggatct gctgtgccgc aggctcttat
     7561 ggaggcgttc gaggaccggc tcggcgtgac catcttgcag gcgtggggca tgaccgagac
     7621 ctcgccgatg gcgtcggtgg cgcgcccggc ggtcactgcg agctcggacg actcatggcg
     7681 tatgcgagcg acgcaggggc gcccggtctg cggcgtcgag atgcggatcg tcaccgagga
     7741 cgggacgccg cagccacgag acgggcgatc cgtcggcgaa ctggaggtgc gggggccctg
     7801 ggtgacggcc tcgtactatg aggtcggcga tgacgcgcgt ttccatgacg gatggctgcg
     7861 gacaggcgat gtcggttaca tcagcccaga tggcttcctg acgttgaccg atcggatgaa
     7921 ggacgtgatc aagtccggcg gcgagtggat ctcttccgtc gagctggagt cggcgctcgc
     7981 ggcgcatccc gatgtgttcg aggcagcggt catcgcggta cctgatgagc gatggcagga
     8041 acgtcctctg gccgccgtcg tcattcgtga aggggcgacg gcttcgcctg gtgaacttcg
     8101 gacatggttg tccggcaagt taccgcggtg gtggctaccc gagcggtgga gcttcatcga
     8161 gcagattccc cggaccagtg tcgggaaatt cgacaagaag gtgctgcgcg gggattacgc
     8221 gggcggtcga ttgaaggtgg aaaccctcga ttgacgtctg cgccatcgag ttcgcggtat
     8281 tggccaaggt gaggaggtga tcgcaatgga cgattccaag agtgcttcga agctcgagtt
     8341 cgacggcgtc acgtcggtgc tcggcgagga accggtactg cacagactac ggctgtcggt
     8401 accggcggga cagatcacgg tcattctcgg gcagccgggg agtggcaaga cgactctcct
     8461 gaagcacctt gtcggcatac tgccgccgac cggaggcagt atccgactcg acggcaggga
     8521 cgtctggtcg atgacctcgg acgaactcgc aagtgcgcga ggcggaatcg cagccgtgat
     8581 cggtgggacg cacttggacg gcagccggct gttctcgtcg atgagcgtct acgagaacgt
     8641 cgcttgcgta gcacgtcatg ccggtgcggc tgacgacgag gtgcacgcgg ccgttgcccc
     8701 gctgatacag gaattcgggc tcgaagtctt cgcggactcc ttccccgaag agattcccgc
     8761 gcacgcgaga cgcagggtgc tcctcgcgca ggttttggtg tcgggcatgc cactgatcat
     8821 cctcgacgac gtggacacca catgcgaccc ccgttacctc gatgccgtcg tcagggcgat
     8881 caagtcggtc aacgagaaga gacgggcaac ggtactgatc acgacgaatg acctgagctt
     8941 cgcgcgtggg gtggctgatg aggtcgcagt cctctggaac ggcaagatcg cttccctcgg
     9001 gccgccagac gtcctgttgc gtggcgtgcg gaccggcgac gagttcgggc ggcggttctt
     9061 ccgatctgat cttgccgggc cactgcaggt cgatgtcgga ggaggtgatc tgcacggcga
     9121 tgagcgcgcg agacgattct tctccgtcga tccgggtcgt ccggtgtggt gtgtagccgc
     9181 gctcctgcta ctggtggttg ttttcgtatt ctggtcgagc gcgggcacca tctttggcag
     9241 gccatgatga tgggcacgct catggctgat ccgtatctgg acagcgtgta cacgtccgag
     9301 ctggcggcct tggctcacgc gatcgagatt gtcgatgatc acgccgatct caattctcgc
     9361 tatcgaggac tgatcgatga ctcgcgagtt tcacttggca gcggccggct ccgcctgacc
     9421 caggcgcggg gccttgccaa gcggctggtc gttctggccc gggcggccgg aaccgacatg
     9481 cgagcccagc tcacaaaaac ggaacgagac gcgttggatg cggggcttgc tcaagcagcc
     9541 gttctgatcc gcattccgcc gccgtgatcc tacgagattg gccgagtagc taggagcgtg
     9601 aaaatggcga gcatcgaatg ccgttgcctg tgtacacccg cgggacacca cgcgaatatc
     9661 tgtacgcaac tggcgagccg ggacaagttc gtgccgttgg tgggcgcgca gccgtcagtc
     9721 cgcgttccag cttgcggccc ttgccactgg gcaacggtca ccgcggcggc cggtcatctt
     9781 ccgcccatgg gacggatggt cgcgcggctg ggaatccggt ccggcggctc gtagcggatg
     9841 gacccgccag ttgaacgaca ttcttcagtc catatccaag cagtaacgtt cgaaggagtt
     9901 ccgatgactg agaagatcgc cgaacaggca actcccgccg aagacaccgt ggtcgccgag
     9961 gacctgctgg tcgaggaggt ttcgatcgac gggatgtgcg gtgtctactg agggccgcgc
    10021 gttcgacctg gacgcgggct ggcgcctgga cgaccgggtc gccgtccggc ccgagcggtt
    10081 cggggcgctg ctttatcact tcggcacccg tcgcctgtcg ttcctgaaga gtccggcgtt
    10141 gctggacgtc gtccggacgc tggccgacca cccgacagcc cgcgccgcgt gcgcgggggc
    10201 gggggtcggg ccgccggagc tgccgcgcta ccgcgccgcg ctggccgaac tggccgtgtc
    10261 acggatgatc cagccgagga gtgtgtgatg tcccttgtgg acgagttcca gtacgggctc
    10321 gatgccccca tctgcctcac ctgggagctg acctacgcct gcaatctttc ctgcgtgcac
    10381 tgcctttcct cgtcagggcg gcgggatccg cgggagctga gcaccgcgga gtgcaaggcc
    10441 ctgatcgacg agttcgagcg gatgcaggtc ttctatgtca acatcggtgg cggtgaaccc
    10501 actgtgcgcc cggacttctg ggagctggtc gattacgcca ccgagcatca cgtcggggtc
    10561 aagttctcca cgaacggtat caagatcact cccgaggtgg cgcgccgcct ggccgcgagc
    10621 gactacgtcg acgtccagat ctccttggac ggtgccaccg ccgaggtaaa cgaccacgtt
    10681 cgcgggccgg gttcgtacgc cacggcgatc cgtgcgatgg agaatctggc agccgccggg
    10741 ttcgagaact tcaagatctc cgtagtggtc acgcggcaca acgcgggcca gctcgacgag
    10801 ttcaaggcga tcgctgatcg ctaccgagcc cagctacgga tcactcggct gcgcccgtcc
    10861 ggccgcggcg ccgacgtctg ggacgagcta cacccgaccg ccgcgcagca gcgggagctt
    10921 tacgactggc tggtggcgcg cggtgagaac gtccttaccg gggactcgtt cttccacctc
    10981 gcagggtacg gcgacggcgc gctgcccggg ctgaacctgt gtggtgccgg gcgggtcgta
    11041 tgcctggtcg atccggtcgg tgatgtctac gcctgcccgt tcgccattca cgacaccttc
    11101 ctcgcaggga acgtccgcga cggcggcgga ttcacccgcg tgtggcgtga atcggagctg
    11161 ttcaccgaac tccggcgccc gcagagcgga ggtgcgtgca cgtcgtgctc cgcgttcgac
    11221 agctgccgcg gcgggtgtat ggccgcgaag ttcttcaccg gtctgccgct cgacgggccg
    11281 gatccggaat gtgtgcgcgg ccacggcgaa cgcgcgctgg ccggtgtcgt cgccgggagt
    11341 gttccgaagc cgtcgctgga ccactcgcac cggcgccgtc ccgtcccgat cactatcggg
    11401 atgcgccgcc cgccggaacg agcctgcgac gagaaccccc tcgccgggtt cgccccgccg
    11461 gggcgctgaa tcgtgcggct ggcggacctg tcctggccgg acgtcgcgga acgcgcggcg
    11521 tccggtgcga tcctcgccgt cccggtgggg gcgaccgagc aacacggccc gcacctgccg
    11581 ctgacgaccg acaccgacat cgcgctcgcc ctgtgcgatc ggctggccgg cacacgtacg
    11641 gacgtcatcg tggcccccgc cgtcgcgtac ggctccagcg gagagcacgc cagattcgcc
    11701 gggacgctgt cgatcggtca ggaggcgatc gaactcctcc tgctggagct gggccggtct
    11761 gcggcggaga cgttccaccg cctgttgttc gtctccgccc acggaggaaa cgccgggccg
    11821 gtcgcccgcg ccgtcacccg gctgcgcgcg gaatcccggg atgtcgaggt cttccagcca
    11881 cgatggtccg gcgaaccgca cgccggacgg ccggaaaccg cgctgcagct ggccttgcgg
    11941 cccgaggcgg tccggatgga acgcgcggtg ccgggggacc ggcggccgat cggagagctg
    12001 atgccacacc tgctcggcgg cggggtgtgg gccgtgaccg cgaccggggt gctcggcgac
    12061 ccgaccgggg cgaccgccgt tgaaggcgcg gtgttgctgg accggctgac cgatcaactg
    12121 gtgtctcatg tggacggatg gggtgggcga tgacctcgcg cacagccctc gtcaccggcg
    12181 cggcccgagg gatcggcgcc gcgactgtcc ggatcctggc ccggcagggg tatgcggtgc
    12241 tcgccgtcga ccttgccgcc gacgacccca cgctcccgta cccgatgggg agcaaggccg
    12301 agctcgccgc cgtcgtcgaa caggcaggcg ggaacgtcga agctttcgta gccgacgtaa
    12361 ggaatttctc ggcggtagcg gcagccgtcg ctgaagccga acggcggtgg ggcgggcttg
    12421 acgtcgccgt cgcggccgcg ggtgtcgtag ccggcggcgt cccgctctgg gaggtaccgc
    12481 cggagcagga gcgggcggtg ctggaggtcg atctgaacgg tgtcgtccac ttggcccgcg
    12541 ccgcggtgcc tgcactgctg aagcgcccgc ggccccggtc cgggcggttc ctcgccgtcg
    12601 cgtcggccgc ggccacccgg ggcctgccgt tgctcgccgc ctactgtgcc gccaaagcgg
    12661 gtgtcggcgg cctgatccgc gcgctggggg ccgagttggg ggagagcggc gtgacggcca
    12721 acgccgtcag ccccggttcg accgacaccc cgatattggc cgaaagtgcc cggctctacg
    12781 acctgcccgc cgcggtcgcg ttcgccgccc agcaaccggt ccaccggctg ctcgaccccg
    12841 acgaagtggc cgcggtgctg gcgttcctcg ccggcccgga cagcggtgcg atgaccggcg
    12901 cgatggtgcc ggtggacgga ggattggcgc tgtgacgata cccctgcctc ccggcttccg
    12961 gatcgacctt gacccggacg tccggaagct cgccgacggc ctctggttcg gcggctcccc
    13021 tgcgcgcgtg ctgcggctga ccgcggccgg gcaggccgcg tggcgggtgc tcgacgccgg
    13081 tcctgtgacg acggcggcga ccggtgtgct ggcccggcgg ctcactgacg ccgggctcgc
    13141 ccacccggta ccgcccgcgc aggacggccc gccgggcgtg accatcgtgg tccccgtgcg
    13201 tgaccgcgcc gaattgctcg cccgctgcct ggccgggctc ggcagccggc atcccgtgct
    13261 cgtcgtcgac gacggctcgg ccgaccccgc cgccatcgcc gcggtggccg ccgcgcacgg
    13321 cgcgaaactg gtatgccggg acgtcaacgg aggccccggt gcggcgcgga ataccgggtt
    13381 ggaacaggtt tccaccgagc tcgtggcctt tgtggacagt gactgcgtac cgccggagga
    13441 ctgggcctgc ctgctcgccg cgcacttcgc cgacccactg ctcgccgcga tcgcgcctcg
    13501 agtccgggcg ctcgcccagg acacctggtc cgggcggtac acccgggccg caggctgtct
    13561 cgacctcggc gaccggcccg cgcgcgtcgc gccgggtacc cggatgtcgt acgtgccgac
    13621 cgccatgctc gtggcccgcc ggagcgcgct ggccgccgtc gcggacggcg gccaggtgtt
    13681 cgacccacgg ctgcgcgtcg gcgaggacgt cgacctcgtc tggcggttgc acgccgcggg
    13741 cttccgggtc cgctatgccc cggacgtcca agttggccac cacgaacccg cgtcctggcg
    13801 cgggcttctc ggccgccgcg tccgctatgg cgtatccgcg gcgccgctgg cgctacggca
    13861 tccgaaggca ctggccccgc tggtgctgca cttctggccg gcgctgaccg tcggagcgct
    13921 gctcgcccgc cggccgctgc tcgccgctgt ggcgttcggc ggctcggtgc gcagcacggt
    13981 ccggctcttg cgcgcgaacg acgtcccatc ggacggagtc gtgcgggcgc tggccaccgc
    14041 cgtccaccag acctggctcg gctgtggccg ccacggcacc cagttcgccg caccgctgct
    14101 cgtggcactt ctggtgccgg gcggacggcg gcgatggggc cgccgtgccg ccgtggcttc
    14161 gctgctgctc ggcccgccgc tgaccgcgtg ggcgagcaaa cgaccgaaac tcgacccgat
    14221 ccgttacacg gccgccgcga tcgccgacga catcgcctac ggcgccggcg tctgggccgg
    14281 ctgcctgacc agccgcaccg ccgcaccggt ccgcccagct ttcctccggc gcccgctgcg
    14341 tttcgacccg aagggaaaac gatgagcacc tggttcgagt ctgtcgccga agcccagcgg
    14401 cgcgcgaaga agcgactgcc cggctcggtc tactccgccc tgatcgcggg ggctgagaaa
    14461 ggcctgacac tgagcgacaa cctcggcgcc tttgacgagc tgcggttcgc tccccgcacc
    14521 gccgggctgt cgggtgaccg cgacctcagc acacacgtgc tcgaccggga cgtcgcgctg
    14581 ccggtgctga tctccccgac cggggtgcag gcggtgcacc cggacggcga ggtcgccgtc
    14641 gcccgggcag cggccgcgcg cgggacgtcg atgggactga gctcgttcgg cagcaagccg
    14701 atcgaggagg tcgtcgccgc gaacccgaac acgtacttcc agatctactg gaccggaacg
    14761 cgccacgaca tcctccgccg cctcgaccgc gcccggactg ccggggccgt cggcatcatc
    14821 ctcacactcg actggtcgtt ctcgcacagc cgcgactggg gcagcccgca catcccggag
    14881 cggctcacct tccgcgagct gctgcggttc gcccctgagg ggctgacccg acccgggtgg
    14941 ctgctggact acgcgaagac acggcggctg cccgacctga gcgtgccgaa tatggtcgcc
    15001 ccggacgagc aggcacctac gttcttcggc gcgtacggcc agtggatgca aacgccgccc
    15061 ccgtcgtggg aagacgtgcg ctggctgcgc gcgcaatgga acgggccgtt cctgctcaag
    15121 ggaatcatcc gcgccgacga ggcccggcgc gctgtcgact ccggagtcag cgcgatctcg
    15181 gtgtccaacc acggcggcaa caacctcgac ggcaccccgg cgacgatccg ggcccttccc
    15241 tcgatcgccg acgcggtagg cgaccaggtg gaggtgctgc tggacggcgg catccggcgt
    15301 ggcagcgacg tggtgaaggc actggcgctc ggcgcgcggg cggtcctcat cggccgcgcc
    15361 tacctatggg ggttggcagc gaacggccag ccgggtgtgg aaaacgtcct cgacgtgctg
    15421 cgcaacggca tcgactcgac gctgctggca ctgggcaaga agagcgtccg agacctgact
    15481 cgtgaggacg tgcttgtccc ggagggattc gagaccgaac tcggtgcaac ctcggccgca
    15541 gaccggagcg ggctgtccgg ttagggccga agaagatgat cagcggtggg ttgtgggcgc
    15601 tcgcggcggg tctggtgtgg ctgtcgctcg tcagcccgac ggggcgattt cgtcgtcggc
    15661 gtcctgcccg cgtcgctggt cgcggcactt ggtcaggcgc cggcgttcaa cccgtcgctg
    15721 ggcaccgcga tctcgagtgc gaagccggag ataggcgggc tcgcgtcggg catcgtgaac
    15781 acctcgtacc agatcgggtc ggcgctcggt caggccgcga tgaccgcggt cggcgtgtcc
    15841 tttcggcgcg gggcggatcg gagggccagc ccgctcaagg gttacgccct tcacgcggcg
    15901 tttcgccatt tccagcaata cgcttagaga acgaaacgcc gctgggagga tgaccgtggc
    15961 aggtggaggc gatggccgca cgtcggaccc gctgtcggtg gtgactacca gagcgggatc
    16021 tgggcacgtg agcatcaccg ttaccggcga gatcgatctc gtgacgaccg agttcctcaa
    16081 aggggaattc agtcaggcac tcgatccaac gccgaccagc gtggtcgtcg atctgaacgc
    16141 ggtggacttc tgtgatacca ccggtctatc ggttctcgtc ggactcaaca atcgctgcgt
    16201 cgccgaccac atcgcccttc gggtcctgcc ctcggcgagg atccaccggt tgctgctgcg
    16261 tactggacta tcgaacctgt tgccgatccg ctgagtcagc tatcagcaga acgcctggcg
    16321 cgccatccga aggagtggat catgtccttc ccgtgtcccg ggcgcggcag cgacgaggcc
    16381 atcagttgcc atcaaagtca gtccaccgag aactctttgt cggcgccgcg agcgttgagt
    16441 tcgggcgacg acggctcatg gccacactgg acccgggtct gctgctgggt tcatcgcgtg
    16501 ggcaaggtgg cgggatccac gatggcggtc acggcgatgg ccgcggcgat gagcaagacg
    16561 agatacgcga acccgcggcg gaccgcgcgc tcaggcagac gggtcgccag gcgccccgcc
    16621 gcgagtgcgg cgaggacggc ggcaccgccg aaaatcccgg tagtggtcca gtcgatgggg
    16681 gcgtcggcgc gggctgccag gcctgcggcg gagttgatga cgatgatgac caatgacgtg
    16741 gcgacggcct gggccgcgtc caggccgagc agcagggtca gggcgggcac gatgaggaat
    16801 ccgccgccga cgccgaacaa accggtcagc accccgacga ccgcgcccgc ggccagagcc
    16861 ttgggcaggc aggctcgcca gttgatccgg ccgccgctcg tgcggcaggc accgccgctg
    16921 ctgcggtgtt cggtgagcat ccggaccgcg acgaccgcca tcaacgcggc gaaacccagc
    16981 aacagcacac gcggcggcag tgaccggccc agcgccgcgc ctccgaaggc ggccgggatg
    17041 ccggcggcac cgaagacgag cgcgatcggc caccgcacgg ctcgggtccg tagccgcggt
    17101 atcactgccg cggcggagga cacgccgacg atcagcaagg aggcggggat ggccgcggcg
    17161 aggggttgtc cggcggcgta gacgagggcg ggcaccgcca ggatcgaccc gcccgcgccg
    17221 agcaggccca gcgcgagccc gatgagcagc ccggccagcg cggcggcgat caacatcggt
    17281 caggctgcca tcggcacgcc ggcggtctcg gcgtggccga aggcatcgtc gatgagcacc
    17341 gcggtgcggc cggcgcgggc cagcagcgag gccgcggcgg cggcccggta gccgcttccg
    17401 cagtgcaccc acaccggtcc tgcgggtagg tcaccgagcc gctgtggaag ctccggcaga
    17461 gggatgtgta ccgcgtcgcg cacgtgggac tcgcgccact cgtggggcag ccggacgtcc
    17521 agcaccacgg cgggcgccgg aagcccgtcg gggccgcggt gcgccaaggc tgcggccagg
    17581 tcgccgaacg acgccgtcgc cagcgcggcc agccgctcgg cgtggtcgcc ggcccactgg
    17641 gcggggtccc cggtggccgc ggcggcgggg cggtcgatcc cgatccggac cagttcccgc
    17701 tgtgcctggg cgacctggtc gggggtgtcg ccgagcagcg tgatcggggc accccagtcg
    17761 atgagccagc cgagccaggt caccattggc ccgtccaggc ccaggctgac cgtcccggcg
    17821 aggtgcgagc gcacgaacgc cttgcgtgag cgcaggtcca ccacccattc cccggcatcg
    17881 acccgccgga gcagctcgac cggatcggcg gtggccaccg gggtgaggtc gatcggctcc
    17941 gggccggcgg tattgcggac gcccatgtgg gcgtagtagg cggggtagtc gtccagcccg
    18001 gcgagggtct gttccacgaa ctcggtttcg gccagcgtca gtgccgggtt gatctgtcgt
    18061 tcccgcccga tcgtggacgc gtccccggag gcctgggtgg ccgagcagaa gctgccgaag
    18121 ccgtgggtag gccacacctg cgccccgtcg ggcagcagct gcgccagccg gtgcaccgac
    18181 gaacgttgct gccgtgcgag gtcgtgcgcg tggtcctggc ccagcaggtc ggtgcgtccg
    18241 gtcgtgccca gcaggagcga gcccccggtg aagacccctt ccggcccccc tgggccgtcg
    18301 aggatgtagg acaggtggtg aaaggtgtgg cccggcgtcg cgaccggccg gatgtgcatt
    18361 cgctccgaca cgttcagcac gtcgccctcg tgcagcggca cccggtcgaa cggcacctcg
    18421 tcgtcggcgg cgatcccgta gcgggctccg gtcagccggg ccagctgcag gccgcccgac
    18481 acgtagtcgt tgtgcacgtg ggtctccaga acccacgcga tccgcacgtc gagccggccg
    18541 gccagcgcga gcacccggtc gatgtcacgc tgcgggtcca ccacgaccgc tacctcgtcg
    18601 tcgtgcgcca ggtagctgcg gtcgcccagc gacgacgtct cgatcacttc tacgtccagc
    18661 atgcgattgt ctccttcggg agtcattgcg tggtcagttc cggcccggtg cgcacgggcc
    18721 ggccgctgcg ctgccagccc gcggtcccct cggcaaccga taccgtgtcg acactcatcg
    18781 cggccaacgc ctgggcggcg cgcaggctgc ggtttgccgg actggcggat cacccacacc
    18841 tgctgcctgg cccattcccg gccggccgtg ggcacctgtt ccaacggcac attgaccgcg
    18901 ccacgcacgt gtgcccgggc gtactcaccc ggcgaacgca cgtccaccaa ccgggctccc
    18961 acgctcagcg cccgatccac gtcaccgacg gctgcttcat ggactgccat gccttacctc
    19021 tgccctgctg taccccgggg ggtatgtgcg aaacacacgt ccgcgctgtc cgccaatacc
    19081 ggcgctcagg ccaaggccag gaagagtttc tccagttcgg cctcggtcat cgagggttcc
    19141 tcgccgcggt cccgggcggc ctggcagtac cgcatgccgc tggccaccac cttgaacccg
    19201 gcccggtcca gcgcccgcga caccgccgcc agttgggtca gcacggtcgc gcagtcctca
    19261 ccggcctcta tcatgccgat caccgcggcc agctgcccct gggcgcggcg cagccgcacc
    19321 agcgcgtcac cgaccagttc aggcgtcatc tccatgcccc acctccaacg tcgccagcct
    19381 accccccggg gtatgcagtt cccgcgaagg gtggacgcga tccggggcgg agcacgtcag
    19441 ccgcgcccga acagccgacg caacacccgg gtcaccccgc ccggttcggg ctggcactgg
    19501 caccgctgcg cggccggcac gccggccaat acctgctcca cgtgctgccc gcatcccgcg
    19561 taggtggtct tcccgcaacg cgaacacgtc acccgacggc acatcagtga acctcctcga
    19621 ttcgatgcct cttacccgca gggtaccccc cagggtatag agcggacaga gtcgtgcgcc
    19681 acaccgatgc cagcggctgc gttctgtccg attccgccgg tgcggcggca aggccgacgc
    19741 tgctagatga ttgattccgt gaagatcgtg tgaggtgtag ggctgttcgt ccgctaaggc
    19801 tgtccctgct ggggccgcgg gggagccgac cgtgtctcgg gtgtccacaa aggacacttc
    19861 ttggcggcgg gaggaccgat tcatgtccat atgtacggtt ttgcaggggg tcgtgagtgt
    19921 tttgtgtcgt tctaaccaca caaaacactc acgacccccg atatcaacac cagatcacgg
    19981 ctgccatggc cagtaggcgc tgcgcgacgc gggtgaacac gccgtgcggg gtgcgtccgc
    20041 cgtgtgcttc gaggtcgagt tggcctttga gggtctggtt gaccgactcg atccgctggc
    20101 ggacgccacc gaggttgcca ttgcgatagg tctcgtcttt gcggtccggg cgcagcagct
    20161 ccagccccat ggactcggtc agctgtttga agtccttacc ggcgaaacct ttatcgccaa
    20221 caggacttgg ccttcacgga tgaggtgatg gttgtggttg agcagggccg ccggcacctg
    20281 ccgttcgccg agtttcgggt tggccaggca ccacatgatc ggcatcccgt caccggcgca
    20341 gacgagatgg agcttcaggc cccagtaata acgggtgtgt gacgcgcagt agccgtagcc
    20401 ggcgtgcccg gccaggtccg agcgcttcac tgtttcccgc gacattccgc agggcgccgg
    20461 ggtggcgtcg gtgatccaca ggtcgtcgaa ccaggacggg cagcacgcgg ccaccgtgag
    20521 gattgcttta cacagcaacg gttgtgccgt cttcagtcgc ttgtggtatc cggactggcc
    20581 cagcatggcg gggaacatcg accgccactc ggagtcggcg tggatgcgcc ggatccagcg
    20641 ccgctcgctg tcgtagcgct gcagaacctg cgcaacagcc atcgtgatca gctcactgtc
    20701 ggtcaactcc ggtcgcctcc cacgaccggc ccggggcggc accacatgat cgtcgatcag
    20761 cacgtaaagt gcgaccaaga gggtgttcag ttctttcgtc acggtttgat cctggacacc
    20821 ctcctcccga gcccacacag cggctccctt cacgcgatct tcacggaatc agtcatctag
    20881 gagagaaagg agtcgcccgc gacgtcatgg gcggcagagg gcgggagcaa acctcgtgcc
    20941 tccgctggac tcgtttcacc aatggcgatc gacgcccgga gatgtagtca ggaggacctg
    21001 gacggtggcc atcattccgg cgtcctcgtc gtcgaagatg tggcagtggt agacaatgcg
    21061 gccgggaaag tcggcgaact gcacgcgcac gtcggccacg atgatcgcgc cgaagcgtcc
    21121 gccgaagagc tggtcggcga cgaaacggtg cccggttcgg gttggtacca ccaccccgcc
    21181 gccgcgctcg ccgagcctgc caccaggcca acgcgcggcg acggccgacc tggccgccgt
    21241 tcattccggc tcgcccggcg gcggtactcg tcctggtcga tctcgccccg cgcgaacgct
    21301 cgtccaggat ccgccgcgta ctcccgccag gtcgatccat cgaggtcgag cgccggctac
    21361 gcgcccacac cacgccagcg gccatcgcca gggccaccgt cacattcagc cgatcgaggc
    21421 accgcccatc gtcgcctccg tgccgggacg gtgacgtgga acgaatccgg ttaccgctca
    21481 cccaccgcct gcaaggccgc ccgcaaccgc cgtccggcct cgtccgcgat cggctgcagc
    21541 cgcgaatcct ccggcacgct gacgatcacc tgcggatcca gtgcttctac catggtcgac
    21601 tcaccgtcca cgcgcaccac gacgttgcac ggcagcaaga gcccgatccg gcgatcgaca
    21661 ccgagtgctt gatgcgccag ttgagggttg cacgccccca agatcaggta cggctcgctc
    21721 tcctgcccaa gcttcgcctt caacgtcgcc tgtacgtcga tttcagtcag taccccgaat
    21781 ccctgatccg ccagcgccgt ccgcacccgc tcgactgtct ccgccatcgg cgcggcactc
    21841 cggaccgcga tgccgtagtc cattctcatc acctctcgtg ttcgttgatt gtcggaccgc
    21901 taccgaagat acacccgggg gtatctgatc agggcttctc cgcaccaccc atgccagcag
    21961 gtatagccga acggatgctc cggtgtcgcg gcccgcttcg cgcgtttgcc gcccagtagc
    22021 agcaatgcct ggttgctggt gtcggccagc gagtgcacgg actccgcaag catcgaggag
    22081 gacgcggtga gcaggaacgc gacgaatttc gtcaccgcga tccccaggtt ggcgaacatc
    22141 gccgtgacaa ccgcccgcgt acttccttct gcggccacgt atgcacccta gtgtcgttga
    22201 ttcgtttacg tctgcgggtg tgggtgcggg agaatcgccg catgtatgtg gcggaggcgt
    22261 tgcggttgcg tgagggtgat cgtgggcggt tggaagggat catctgtgcg tcgaccgcga
    22321 ctccgccggc gtagcgatgc cccacgacgc gaagcccgtg tttggccgcg tgttctggcg
    22381 atccgtccgg tgtcgggcag acagtgcccg acccgcccgc gaacggggtt gccggaggct
    22441 ggagaccttc atcgattccc gtgttcagcg tgctctccat ggtcggagac ctgcgcttcg
    22501 ccccaggcgt gcagtgctgt gatgagggca ggatcgttgg tgtggaagga aagtgaagcg
    22561 ccgtccggta gttcggcgta gtcgatggtg atccgtggcg ccccggcggt gagttcggtc
    22621 aggccgggca tggtgccgcc gtggatgctg gcggggtcgg tgaagtctcc gctgcggaac
    22681 cgtgtgctct cttcggtgag gtgggtgcgg atggggccga tttgcgccat gtcgcccggc
    22741 acgtcggcga cgacttcttg aaggaggccg tctgggcgag gtgtgaagcg gtgcgttgtc
    22801 ttctcgaggt cgaacggcat gacggatctg cccttctcgg cgacctctga ttgacgggcc
    22861 gactgtggct ctgtggctgg tgtcgagcat gcggcgccca gggtcgcgac gaggacggcg
    22921 gcggcgatgg ctctacgggt ggtcactggt ggtggcctct ttcagtaggc gtagcgcgtt
    22981 gatcacgttg gtgtgctgtt cggggtcgat gttgctgagg agttgggcaa gccgtttgcc
    23041 gcgtcgctcg acgactccgg cggagacctc tgtgccggag tcggtgagcg acagcagccg
    23101 ggcccggccg tcggcggggt cgggccggcg gagtacccag ccgcggtcga cgaggccatc
    23161 tgtcaggcgg ctggtggtcg acttgcccaa attcaagcga gagcccagtt cgccttggtg
    23221 tagcgggcca tctcgcagga tggtcagtgc gtgcgcctcc gccacggaca tcggagatcc
    23281 acagggtgtg cggtctggtt cgagcagccc cagggaccgc acgagactga cgaacgtttc
    23341 ggcgaactcg tgggcggcgg ccggctcggg gctactcgac atagttccaa tctagaacta
    23401 tatagtccca agatgcaaca aagttcgcgg gagcggctcc gattcggggg gacggggcgg
    23461 gagaggcggg tcaccacgtc acggggaggg cggtcaggcc gccggtgagg acgtctcgtc
    23521 ggatggtcag gtcttcgacg ggcacggcaa ggcgcatggt ggggaaccgt ggaatcagtt
    23581 gggtgaatac ggcttgtagt tccatgcggg ccagaggcgc gccgatgcag tagcggaggc
    23641 cgtgcccgaa ggtcaggtgc gccgaggcat ggcgggtgac gtcgacgtgg tcgggctctg
    23701 cgaagacggc gggatcgtgg ttggcggcgc cgttgtcgag cagcacgagt tcgcccgcct
    23761 ggatcgtgac gccgtcgatc tcgaagtccg ttcgcgcgta tcgggggatc ccgccgccgc
    23821 tcttgcctgg tgcccggagg agttcttcga tcgcgttcgg gacgagaccg ggattgtcga
    23881 gcagggcgtt ccattggcca gggttggcga gcaggagcag cgcgcccatg ccgatctgga
    23941 ccacggtggt ctcgtggccg gcgaacagca atgccatcga cagcatggcg gcttcggagt
    24001 cggagacacc gtcggtcgcg cacagccggg agatcacgtc gtcgcctggg gacttgcgct
    24061 tgtcgacgac gaggcgcatg ccgtagccga acagctcgcc cagtccctgt tcggatcgtg
    24121 cccggtcgcg gatgtcaccg gctgcttcgg tccaggcgcg gaactggtcg cggtcctcgt
    24181 acggcacgcc gagcagttcg cagatcacca ggatcggcag cggtaaggcc agcgccgtat
    24241 gcaggtcggc gggttgcggg ccgccgtcca ggtcgtcgag cagttgtgtg gtgagtgtgt
    24301 cgatgcgggg cctgagcgct cgcatgtgtt tgggggagaa gtgcggttgc agcagggtgc
    24361 gcatccgggc gtggtgggtg gactcggtgt cgaagtcgcc gtgcgggcca ccgaacaacg
    24421 cggactcgcc ggttcgcgcc gcgttctcgg gttcgcgatg ggatcggccg agccggtcgt
    24481 cggagagcag gcgtcgcact tgcgcgtggt cggtgaccag ccaggcctcg tcgccgacgg
    24541 cggtgcggat ccggtggatt ggtccctggg cctgcaaggc gcacagctgg ggtgcgaccc
    24601 gcagcaggtc gggctggtcg aaagggagtc gggctgaagc gctcacgatg aacctctttg
    24661 tgaactcaca gttgtatacg ttgacgtaaa caaacgtact tgtatactcc gatgttgaca
    24721 agtggaggtg cgatggctga acccgagccc gtccggcgca tgccccgcgc cgaacggcgc
    24781 gagcagatcc tggacgcggc gacccgggcg ttcgcgcgca cggggtatgc cgcgaccggt
    24841 ctggatgacg tggccgcaga ggcgggaatc acgcgtgtgt tgctgtaccg gcatttcgac
    24901 tcgaagggcg acatgtaccg ggccgtgctc gaccgggcct gcgcccggct cggcaccgcg
    24961 gtgggatccg agggggacta cgacgagggc agcctgcccg cgctgatgcg cagtgccgct
    25021 gacgatccgg acggattccg gctgctgttc cgtcacgcgg tgcgggagcc ggagttccgt
    25081 gacgtggtgg acggtatccg gtccgcctcc gccgaggtca cgcgcaagga gctcgccgcg
    25141 atgatcccgg acggcccgtg gctggactgg gcggtccagt tggttccggc ggtcacgctt
    25201 gaggcggtga tcgcctggct cgacgcggga cagccggatc cggaccaggc cgcgggccgc
    25261 atcgcccaga tcgtgcacgg catcgtcgac gtggccgggg ctgttgaccg gccgaaggga
    25321 tatcgagatg agcgacgaac acggcaatga acggcttcgc gacgtgtggg atcgctacgc
    25381 gtcccggtac gaccgcgaca tgggtttcgt cgaacgtctc cagttcggcg gcggccgtga
    25441 atgggtgtgc tcccaagccg gcggcgacgt tctcgaggtc gccatcggca ccggccgaaa
    25501 cctcccgcat tacccggcag gcgtcacgct caccggcttg gacctcagcc ccgccatgct
    25561 cgac
//
