LOCUS       NZ_CP102415            10816 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Amycolatopsis roodepoortensis strain ZEL-1 chromosome, complete
            genome.
ACCESSION   NZ_CP102415
VERSION     NZ_CP102415.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis roodepoortensis
  ORGANISM  Amycolatopsis roodepoortensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Biodegradation of petroleum hydrocarbons by Amycolatopsis orientalis
            ZEL-1, genome analysis and its potential in plant protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) College of Life Sciences, Sichuan Normal
            University, No. 1819, Section 2, Chenglong Avenue, Longquan
            District, Chengdu 610101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024628825.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 06:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,064
            CDSs (total)                      :: 8,994
            Genes (coding)                    :: 8,928
            CDSs (with protein)               :: 8,928
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 44 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1953831
            Orig. end                         :: 1964647
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    506..520
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    complement(604..617)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.74"
                     /tool="antismash"
     gene            679..984
                     /locus_tag="NQK81_RS08900"
                     /old_locus_tag="NQK81_08900"
     CDS             679..984
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033523.1"
                     /locus_tag="NQK81_RS08900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08900"
                     /product="hypothetical protein"
                     /protein_id="WP_126731594.1"
                     /transl_table=11
                     /translation="MADSGPFGPGQNPRFQEDLVGLDPYDPEARAFAEHLDRIERCGPN
                     FTVEACLDGVSDFADSSNRVGGLRWWVSALVVVLIVLGVMVTAWDIIGRAIAWLAG"
     gene            1004..1426
                     /gene="msrB"
                     /locus_tag="NQK81_RS08905"
                     /old_locus_tag="NQK81_08905"
     CDS             1004..1426
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333001.1"
                     /locus_tag="NQK81_RS08905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08905"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_037304420.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLSPDEYAVLRQAGTERPFTGEYTD
                     EKTTGVYQCRACGAELFRSDTKFESHCGWPSFYDPADSDAVLLREDTTMGMRRIEVLCK
                     SCHSHLGHVFEGEGYATPTDQRYCINSISLKLVPES"
     gene            1502..2386
                     /locus_tag="NQK81_RS08910"
                     /old_locus_tag="NQK81_08910"
     CDS             1502..2386
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157012.1"
                     /locus_tag="NQK81_RS08910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08910"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_257487812.1"
                     /transl_table=11
                     /translation="MNEPWGITGPAFAWLYGVCAVVPIAFAVVHELWLRRDTGARTAPG
                     LHHLAALAGGVERVTDTVVAGLLERNLLRPDSRGNLHPIRPVPADPFERAVVSLVPGNG
                     IGLRELRFRMARHDVVRELRADLERRGLFVHEHRRRRGWQYAIAAYVAVFAVGIARLVN
                     AVPLKRPIGALVVLVIVVAGAIGLAVWRQLPERSGRATAAGDLALHYGDWDRDLGRTTA
                     GRVALHGLGAFPDQVVVGALSKTGVFARTASTCGAGASYAVGSSCGGGSSSSCGGGSSC
                     GGGGGGGGGGCGG"
     gene            2379..3215
                     /locus_tag="NQK81_RS08915"
                     /old_locus_tag="NQK81_08915"
     CDS             2379..3215
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="NQK81_RS08915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08915"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_257487813.1"
                     /transl_table=11
                     /translation="MAEPWGISGPDFVWLYGGLVVLPLLFAGLWTWSLRHRAADEGTPP
                     GPYHLAALAGGTERVADVAVATMVAGERIRLDSGGRVTVLRTASGDPLTRAATELVPRA
                     GAGGLYRLKKLLSRSAAVQEVRNELQLSGLQVDERRRRAGWLAALILYLPLLALGVARF
                     ANAMPLGRLAAILAGLLVVVVAAVAGTVLLLRPSRTVLPTAAGLRVLGAARRNPALLPG
                     VAGAVALGGLAAYPDPVMADMLVRAGDATAKGSAGSSSGVSNCASGSSCGGGGCGG"
     gene            3208..3990
                     /locus_tag="NQK81_RS08920"
                     /old_locus_tag="NQK81_08920"
     CDS             3208..3990
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="NQK81_RS08920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08920"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_257487814.1"
                     /transl_table=11
                     /translation="MADFLWLYTPAIAIPALVAALHVRWLRRGFHDGGRLPSVRHVAAL
                     AGGTARVTETVVASMLEREQARLGRSGRLYRTPLVPVDPLGRELAGHLSSFPGHELRTT
                     MSRGPAMRALWAELGGRGLVVAEGRRRLTWRITAVVYAVLLVAGTVRWIVTGGTVVLIA
                     LLVVALALLIVSLLLGRTDYAGRTTTAGRAVLAEAETDRSLVSGAAGAVAIGGFAAYPD
                     REVAAILSRYGGRPGKDTRIFAWGDGTDYSVSGGSGGF"
     gene            4073..5011
                     /locus_tag="NQK81_RS08925"
                     /old_locus_tag="NQK81_08925"
     CDS             4073..5011
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332999.1"
                     /locus_tag="NQK81_RS08925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08925"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_257487815.1"
                     /transl_table=11
                     /translation="MEDTWGIPGPTFLAIYGALIVFPVVVGLVWTIAVKLGAKAPRGTV
                     SGPEPTVYELAYLAGGPDRVVDTAIAALVDRGTLRVSSSKQLQLTGPEPADPIEKAVAK
                     GARPGYNATTRGIRDRLRMSGPMQALAKGLETRGLVVADRTPRIRQVVYFLYLAVLVLG
                     VVRLIAGIAGDRPVGYLIPLLISAVFATVISRALKNKRPGPRPTSEGGRILHRARSAHG
                     RERKRGIPNGPGYAGAAGVGLLGGAAAAVAFGGLASYPDDELSAALIPPPVSGGFGGGS
                     SGGGSSCSSGGSSCSSGSSGGGGCGGGGYGG"
     gene            5001..5816
                     /locus_tag="NQK81_RS08930"
                     /old_locus_tag="NQK81_08930"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033528.1"
                     /locus_tag="NQK81_RS08930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08930"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_126731586.1"
                     /sec_met_domain="DUF692 (E-value: 4.3e-82, bitscore: 274.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAADGPGTLGIGIGWRHELDLSIARLPGVDWVEVVAENLHAGHLP
                     ETLLALRDKGMPVLPHAVSLSLGGAEPLDTARVEHLAEVTRELGAPMASDHVCFVRAGG
                     LDSGHLMPLPRTREALDVLVDNVKLAQSIVEVPFALENIAAVLEWPDAEFTESEFLREL
                     TDRTGCLLIIDVANLYANARNLGTDPERFLDEIPWERLAYTHMAGGVERDGVYHDTHAH
                     PVVPEVLDLLRELRRRTEPPGVLLERDDDYPSDTELAAELAALREAVSP"
     gene            5813..6157
                     /locus_tag="NQK81_RS08935"
                     /old_locus_tag="NQK81_08935"
     CDS             5813..6157
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033529.1"
                     /locus_tag="NQK81_RS08935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08935"
                     /product="hypothetical protein"
                     /protein_id="WP_257487816.1"
                     /transl_table=11
                     /translation="MTPPRERLAAQQAELLKALLAGGDAPAGFDAERLRIEANVLRNKQ
                     SRLAAYLRPDLAEALGDRFAALFREYATNRPKTDAIRARAYADAFGAWLVERGEVPKPR
                     GRLARLLRRI"
     gene            complement(6222..7568)
                     /locus_tag="NQK81_RS08940"
                     /old_locus_tag="NQK81_08940"
     CDS             complement(6222..7568)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033530.1"
                     /locus_tag="NQK81_RS08940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08940"
                     /product="CoA transferase"
                     /protein_id="WP_257487817.1"
                     /transl_table=11
                     /translation="MDTRVWTTLTGDTLAEDAVEITGPESVLPGTFRVEEAATTSIAAA
                     TLAAGELLRLRGIDPGKVSVDTRHAAASFHSEQYIRVDGEPVPFSAPLSGDYRANDGWV
                     RLHCNYPRHVSAVCWGLGVPATRAAFEKVVAAKSKYEVESAVVGAGGAAAVLRSREEWL
                     AHKQGQASSALPVAEIRPLGTSEPVRLFESDRPLGGVRILDLTHVIAGPVAGRVLAAHG
                     ATVMHIGSAQLPTVPPLAVDTGMGKLSAYVDLETEAGRSRLRKLIARADVVLQGFRPGS
                     LVAKGFSPETIAELRPGIVVADLSAYGWEGPWARRRGFDSLVQMTSGIADEGGKAAGLD
                     GPGPLPVQALDHATGWLTAAAIMTAVRRAVTEGGSQHVRTSLAGTGEWLNSLGRKEAGP
                     SGFDGGEWLEDVDGQLGRVTRVKMAGTLPGSNPQWTEATHVAGSDRPVW"
     gene            complement(7580..8272)
                     /gene="hemQ"
                     /locus_tag="NQK81_RS08945"
                     /old_locus_tag="NQK81_08945"
     CDS             complement(7580..8272)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="NQK81_RS08945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08945"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_192745353.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEQGKLGEDREKATAETTEYLDA
                     LEAKGVVVRGVYDLSGLRADADYMIWWHAEEIEQVQAAYSGFRHTPLGRASAPVWSQVA
                     LHRPAEFNKSHIPAFLAGEEARKFICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTKVEP
                     AELIALLP"
     gene            complement(8298..9677)
                     /gene="hemG"
                     /locus_tag="NQK81_RS08950"
                     /old_locus_tag="NQK81_08950"
     CDS             complement(8298..9677)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332996.1"
                     /locus_tag="NQK81_RS08950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08950"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_257487818.1"
                     /transl_table=11
                     /translation="MSTIAVVGGGISGLTAAYRLRTLLGEDARIVVFEATDSLGGKLRT
                     VELAGQRYDVGAEAFLARRPEAVALVREVGLGESLVHPTKARAKIHAGGSVLGLPPGTV
                     MGVPASADAVAGVLSEKGRALVEAEASLSPLKLPAGDVPLGELLRERFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLASAIDRGATSLTEGAASLIPASPSTAPVFGTLLGGLG
                     TLIARLAELSRAEIRTGTTVTALERTPTGWKVGDLDADSVLLAVPAPSARRLLDGVAEV
                     ASTVFAKIELASMAVVALALPPGTALPESSGVLIGAGERDAAGKPFAAKAFTFSSRKWA
                     QYGEGPVLVRGSVGRFGEPGALHADDDELVRVVRDDLARLTGITAEPIDTLVTRWGGGL
                     PQYGIGHLIRVERIERAVAAVPGLAVAGATLHGVGLPACIATADAAARRIAGITPTHEG
                     S"
     gene            complement(9674..10747)
                     /gene="hemE"
                     /locus_tag="NQK81_RS08955"
                     /old_locus_tag="NQK81_08955"
     CDS             complement(9674..10747)
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156993.1"
                     /locus_tag="NQK81_RS08955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_08955"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_257487819.1"
                     /transl_table=11
                     /translation="MSPSVASPAQTVPAARRALPGAPFLAAARGERPAHTPVWFMRQAG
                     RSLPEYRALREGTSMFEACFDPEMLAEITLQPIRRHGVDAAILFSDIVVPLKAAGLDID
                     IVAGTGPVVAEPIRDAAGVRALPVLEPEQVERVAEGVRLLVGSLGETPLIGFAGAPFTL
                     ASYLIEGGPSRNHEHTKALMHSEPELWHDLAGRLADMAITFLSAQLDAGADAIQLFDSW
                     AGALSERDYREFVLPHSAKVLSAVARYGVPRIHFGVGTGELLVAMRDAGADVVGVDWRI
                     PLDEAVRRLGGGVVQGNLDPALLHASWPVIEAEVRRIHAEGRAADGHIFNLGHGVLPGV
                     DPDVLTRVVGLVHELQP"
ORIGIN
        1 gtcgccgcga tgagcccgcc gccgtccgcg gccgccccga tgcggccgtc cccggccaag
       61 cccgccgacg ccaagcgcaa ggccgtcctg ttcgcgggcg ccggtgccgc cgtcgtggtg
      121 atcgccgtga tcgtgttcct ggtcctgaat tcgggcaacg gcaccgacaa ccagggtggt
      181 aacaacgccg gggcgaaccc gcagaccagc tcctcggcgc ccaagtcgag tgcgccgtcc
      241 tcgtcgcgac ccgcgccggc gggcctgggg aagaccccca gcgaaaagat cacgagctac
      301 tcgagcgtgg gtggtttcgt cctcgacttc ttcgacacgg gcaggatcgg gacgtccgcg
      361 agctggaaca tgctcaccga cgccgcgaaa gaggtctacg gttcggagca ggctttcgcc
      421 gagtactgga gcacccacaa ggtcaccagc gccgacaccg cccgggcgga caagggcggc
      481 ggcaaccccg acggctcgat cgacatgaac atcacgctca acaagggacc acgcccggcg
      541 ctgcgcgtgg tccaggtcgg cgggcagtac ttcatcgacg ccgacacgcg agtactcggc
      601 aactgacgca gcgtggcggt ggtgagaccc gcctcgctgg tggtaggcca atcgccgctc
      661 gtggcttact gttgctccat ggccgatagc ggacctttcg gacctggcca gaatcctcgc
      721 ttccaggagg acctggtagg cctcgacccg tacgatcccg aagcccgtgc cttcgccgag
      781 catctggacc gcatcgaacg ctgcggcccg aacttcaccg tggaggcctg cctcgacggt
      841 gtctccgatt tcgccgactc cagcaaccgc gtcggcggcc tgcgctggtg ggtttcggca
      901 ctcgtcgtgg tcctcatcgt gctcggcgtg atggtcaccg catgggacat catcggccgg
      961 gccatcgcgt ggctcgcggg gtaacgtgga accggtgaaa ggcatgaaac ccgttgtcgg
     1021 agccacgccg cgcgtggtca agtccgagca ggaatggcgg gagcagctca gccccgacga
     1081 gtacgccgtc cttcgccagg cgggcaccga gcgtcccttc accggtgagt acaccgatga
     1141 gaagaccacc ggcgtctacc agtgccgagc ctgtggtgcc gaactgttcc gcagcgacac
     1201 gaagttcgaa agccattgcg gctggccgtc gttctacgac ccggccgaca gcgacgccgt
     1261 cctcctccgc gaggacacca ccatggggat gcgccgcatc gaggtgctgt gcaagtcgtg
     1321 ccacagccac ctcgggcacg tcttcgaagg cgagggttac gccacgccga cggatcagcg
     1381 gtactgcatc aactcgatct cgttgaagct cgttcccgag tcgtaaccga aggaagcgaa
     1441 ccaccgccct cctcgacgcg ttcaccttgg acaggacagc gtcggggagg gcggtgtttt
     1501 catgaacgaa ccctggggaa tcaccggacc ggctttcgcc tggctctacg gagtctgtgc
     1561 cgtggtgccg atcgcgttcg ccgtggtgca cgaactgtgg ctgcgtcgtg acaccggggc
     1621 caggacggcg ccgggcctcc atcacctggc cgcgctggcg ggcggcgtgg agcgggtcac
     1681 cgacaccgtc gtcgcagggc tgctcgaacg gaacctcctg cggcccgaca gcaggggaaa
     1741 cctccacccg atccggccgg tcccggccga tccgttcgag cgcgcggtcg tctcgttggt
     1801 acccggcaac ggaatcggcc tgcgcgagct gcgcttcagg atggcccggc acgacgtcgt
     1861 ccgggaactg cgcgccgatc tggagcggcg gggcctgttc gttcacgagc accgccgccg
     1921 gaggggctgg cagtacgcga tcgccgcgta cgtggccgtg ttcgcggtgg ggatcgcgag
     1981 gctggtgaac gccgtaccgc tgaagcgtcc gatcggcgcc ctcgtcgtcc tggtgatcgt
     2041 cgtggcgggc gcgatcggcc tggccgtgtg gcggcaactg ccggagcgct cgggccgggc
     2101 gacggccgcg ggcgacctgg cactccacta cggcgactgg gatcgcgatc tcggccgcac
     2161 cacggccggg cgggtggccc tccacgggtt gggcgccttc cccgaccagg tcgtcgtggg
     2221 cgcgctgagc aagaccggcg tgttcgcccg cacggcgagc acctgcggcg caggtgcgtc
     2281 gtacgccgtc ggatcgtcgt gcggtggtgg atcatcctcc tcctgcggcg gaggttcttc
     2341 ctgcggtggt ggcggtggcg gaggcggagg tggttgcggt ggctgaaccc tgggggatct
     2401 cgggcccgga tttcgtgtgg ctgtacggcg gcctcgtcgt tctgcccttg ctcttcgcgg
     2461 ggctgtggac atggtcgctc cggcaccgtg ccgccgacga agggacgccg ccgggcccgt
     2521 accacctcgc cgcgctggcc ggtggcaccg agcgggtcgc cgacgtcgcg gtggccacca
     2581 tggtcgccgg tgagcggata cggctggaca gcggagggcg ggtcaccgtc ctccggaccg
     2641 cgtcgggaga tccgctcacg cgcgccgcca ccgaactcgt tccgcgagcg ggagccggtg
     2701 ggctctaccg gttgaagaag ctgctgagcc ggagcgccgc cgttcaggaa gtgcgcaacg
     2761 aacttcagct gagcggcctg caggtggacg aacgccgccg tcgcgccggg tggctggcgg
     2821 ccttgatcct ctacctcccc ctgctcgcgc tcggggtggc gaggttcgcc aacgccatgc
     2881 cgctgggtcg cctggccgcg atactggcgg ggcttctcgt cgtagtggtc gcggccgtcg
     2941 ccgggaccgt ccttctcctg cgtccgagcc gcaccgtcct gccgaccgcg gcgggactgc
     3001 gcgtcctcgg cgcggcccgg cggaatcctg ctctgctccc cggagtggcg ggcgcggtgg
     3061 cgctcggcgg cttggccgcg tatcccgatc cggtcatggc ggacatgctg gtcagggcgg
     3121 gcgatgccac cgccaaggga tcggcggggt cgtcgagcgg ggtaagcaac tgcgcgagcg
     3181 gttcttcctg cggtggaggt ggttgcggtg gctgacttcc tgtggctcta cacgccggcg
     3241 atcgccatcc ccgccctggt cgcggcgctt cacgtccgct ggctccggcg cggcttccac
     3301 gacggcggca ggctgccgag cgtgcggcac gtggcggcct tggcgggcgg taccgcgcgg
     3361 gtcaccgaaa cggtggtcgc ctcgatgctc gaacgcgaac aggcccggct cggccgttcg
     3421 ggcaggctct accgcacacc cctggtcccg gtcgacccgc tgggacggga gctcgccggt
     3481 cacctgtcgt cgttccccgg ccacgaactg cggacgacga tgagccgcgg cccggcgatg
     3541 cgggcgctgt gggccgagct gggcggccgc ggcctggtcg tcgcggaagg gcgacgccgg
     3601 ctcacgtgga ggatcaccgc cgtcgtctat gccgtcctgc tggtggccgg gacggtccgc
     3661 tggatcgtca ccggcggcac cgtggtcctg atcgcgcttc tcgtcgtcgc gctggccctg
     3721 ctgatcgtca gcctcctgct cggccggacc gactacgcgg gcaggacgac caccgcgggc
     3781 cgcgcggtgc tggccgaggc ggagacggat cgaagcctcg tctcgggtgc ggcgggcgcg
     3841 gtggccatcg gcggcttcgc cgcgtatccc gatcgcgagg tcgccgccat cctgagccgc
     3901 tacggcgggc gtccgggcaa ggacacccgc atcttcgcct ggggcgacgg gaccgactac
     3961 agcgtctccg gaggcagcgg cggcttctga cgcccggcga atcacggaac cacgaccggg
     4021 cggaagaacg tccgactacg gtaagtgcac ctgctcgcga aaggcccgtg cgatggaaga
     4081 cacctggggc atccccggac cgacgttcct ggcgatctat ggcgccctca tcgtgttccc
     4141 ggtggtcgtc ggtctcgtgt ggacgatcgc ggtcaagctg ggcgccaagg cgccccgcgg
     4201 caccgtgagc ggaccggagc cgaccgtgta cgagctcgcc tatctcgccg gcggcccgga
     4261 ccgggtggtc gacacggcca tcgcggccct ggtcgaccgc gggaccctgc gggtcagcag
     4321 ttcgaagcag ctccagctca ccggccccga gcccgccgac ccgatcgaaa aggccgtcgc
     4381 caagggcgcg cggccggggt acaacgccac gacccgcggc atccgcgacc ggttgcggat
     4441 gtcgggcccg atgcaggcgc tcgccaaggg gctggagacg cgggggctcg tcgtcgcgga
     4501 ccggacgccg cggatccggc aggtcgtgta cttcctgtac ctcgcggtac tcgtgctcgg
     4561 cgtcgtccgc ctgatcgccg ggatcgccgg tgaccgtccc gtcggttacc tcatcccgct
     4621 gctgatctcg gccgtgttcg cgacggtgat ctcccgcgcg ctcaagaaca agcggccggg
     4681 cccgcgtccg accagcgagg gcggccggat cctccaccgg gccaggtccg cccacggacg
     4741 ggaacgcaaa cgcggtatcc ccaacgggcc ggggtacgcc ggagcggccg gtgtcgggct
     4801 gctgggcggc gccgccgcgg cggtcgcgtt cggcggcctc gcctcctacc cggacgacga
     4861 actgagcgcg gccctgatcc cgcctccggt ctccggtggt ttcggtggcg gttcgtccgg
     4921 cggcggctcc tcgtgcagta gcggcggaag ctcgtgcagc agcggaagca gcggcggcgg
     4981 cggttgtggt ggaggcggct atggcggctg acgggccggg cacgctcggg atcggcatcg
     5041 gctggcggca cgagctggat ctgtcgatcg cgcggctgcc gggcgtcgac tgggtcgagg
     5101 tcgtcgccga gaacctgcac gccgggcatc ttcccgaaac cctgctcgcg ctgcgcgaca
     5161 agggaatgcc ggtcctgccg cacgcggtgt cgctgtcgct gggcggggcg gagccgctgg
     5221 acaccgcccg cgtcgagcat ctcgcggagg tcacccgcga gctcggcgcg ccgatggcca
     5281 gcgaccacgt ctgcttcgtg cgcgcgggcg ggctcgactc ggggcatctg atgccgctcc
     5341 cccgcacgcg ggaagcgctc gacgtcctgg tggacaacgt gaaactggcg cagtccatcg
     5401 tggaggtccc gttcgcgctg gagaacatcg cggcggtgct ggaatggccg gacgcggagt
     5461 tcaccgagtc ggagttcctg cgggagctca ccgaccggac gggctgcctg ctgatcatcg
     5521 acgtcgccaa cctgtacgcc aacgcccgca acctcggcac cgatcccgaa cggttcctcg
     5581 acgagatccc gtgggaacgg ctggcgtaca cgcatatggc cggcggcgtc gaacgcgacg
     5641 gcgtctacca cgacacgcac gcccatccgg tggtgccgga ggtgctggac ctgctgcgcg
     5701 aactgcggcg ccgcaccgag ccgccgggtg tcctgctgga acgcgacgac gactacccgt
     5761 ccgacaccga actggcggcc gagctggccg cgttgcggga agcggtctcg ccgtgacgcc
     5821 gccccgggag cggctcgccg cccagcaggc cgagctgctc aaggcgcttc tcgcgggagg
     5881 cgacgccccg gcgggtttcg acgcggagag gctccgcatc gaggcgaatg tcttgcggaa
     5941 caagcaaagc aggctggccg cgtacctccg ccccgatctc gccgaggccc tcggcgaccg
     6001 gttcgccgca ctgttccgcg agtacgcgac gaaccgcccg aagaccgacg cgatccgcgc
     6061 acgggcctac gcggacgctt tcggcgcgtg gctggtggag cgcggcgagg tcccgaagcc
     6121 ccggggccgg ctcgcgcgcc tgctacgccg catttaggcc gtgtctctca aacgcacctc
     6181 ggtctgagag acacgtcttt aggcggctga atgcgggtcg gtcaccagac cgggcggtca
     6241 cttcccgcca cgtgcgtcgc ttccgtccac tgtggattcg agcccggcag ggtgcccgcc
     6301 atcttgaccc gcgtgacccg gccgagctgg ccgtcgacgt cttcgagcca ttcgccgcca
     6361 tcgaaaccgg agggcccggc ttccttgcgc cccaaggagt tcagccactc cccggtcccg
     6421 gcgagcgagg tccggacgtg ctggctgccg ccttcggtga ccgcccggcg gacggcggtc
     6481 atgatcgccg ccgcggtgag ccagccggtc gcgtggtcga gcgcctgcac cgggagcggt
     6541 cccgggccgt cgagcccggc ggccttgccg ccctcgtcgg cgatccccga cgtcatctgc
     6601 accaggctgt cgaacccgcg cctgcgcgcc cacggccctt cccagccgta ggcggacagg
     6661 tccgcgacca cgatgcccgg ccggagctcg gcgatcgttt ccggcgagaa acccttggcc
     6721 accaacgacc ccggccggaa accctgcagg acgacgtccg cgcgggcgat cagcttgcgc
     6781 agccgcgagc gcccggcttc ggtctccagg tcgacgtacg ccgagagctt gcccatcccg
     6841 gtgtcgacgg ccagcggcgg cacggtcggc agctgggccg acccgatgtg catgacggtc
     6901 gccccgtgcg cggcgagcac gcggcccgcc accggtccgg cgatgacgtg ggtcaggtcc
     6961 aggatccgca ccccgcccag cggccggtcg gactcgaaca accgcacggg ttccgacgtc
     7021 ccgagcggcc ggatctcggc gaccggcaac gccgacgacg cctgtccttg cttgtgcgcc
     7081 agccactcct cgcgcgagcg cagcacggcg gcggcaccgc cggcgccgac gaccgccgac
     7141 tcgacctcgt acttcgactt ggccgcgacg accttctcga acgcagcccg ggtcgcgggc
     7201 acgcccagac cccagcacac cgcggagacg tgacgcgggt agttgcagtg cagccgcacc
     7261 cagccgtcgt tcgcgcggta gtcgccggac agcggcgcgc tgaacggcac cggctcgccg
     7321 tcgacccgga tgtactgctc gctgtggaac gacgcggcgg cgtgccgggt gtcgaccgac
     7381 accttgcccg gatcgatccc ccggagccgc aacagttccc cggcggccag cgtcgccgcg
     7441 gcgatactcg tcgtcgccgc ctcctcgacc cggaaggtcc cgggcagcac cgactcgggg
     7501 ccggtgatct cgacggcgtc ttcggcgagg gtgtcgccgg tgagcgtggt ccagacgcgg
     7561 gtgtccacgg aggtcttcac tagggcagga gcgcgatcag ctcggccggt tcgaccttcg
     7621 tcccggtgta gaacgggatc tcctcgcgca cgtggagccg ggcctcggtg ccgcgcagat
     7681 gacgcatcag gtcgacgatg cggtgcagct cgtcggcctc gaaggccagg atccactcgt
     7741 agtcgcccag cgcgaacgac gcgacggtgt tggcgcggac gtccgggtag tcgcgggcct
     7801 ccttgccgtg gtcggcgagc atcttgcggc gctcgtcgtc cggcagcagg taccactcgt
     7861 aggaacgcac gaacgggtag acgcagatga acttgcgggc ctcttcaccg gcgaggaacg
     7921 ccgggatgtg gctcttgttg aactccgccg ggcggtgcag cgcgacctgg ctccacaccg
     7981 gcgccgacgc gcggccgagc ggggtgtgcc ggaagccgct gtaggccgcc tggacctgct
     8041 cgatctcctc ggcgtgccac cagatcatgt agtcggcgtc ggctcgcagc ccggagaggt
     8101 cgtagacacc gcggacgacg acgcccttgg cctccagcgc gtcgaggtat tcggtggtct
     8161 ccgcggtcgc cttctcgcgg tcctcgccga gcttgccctg ctcggcccgg aagaccgacc
     8221 aggcggtgta gcggatggtg tcattgagct cgttgtagtt cagccgcgcc ataccgccat
     8281 cttggcactg ccggtgctca ggagccctcg tgggtcggtg tgatcccggc gatgcggcgg
     8341 gcggcggcat cggcggtggc gatgcacgcc gggagcccga cgccgtgcag cgtcgcaccc
     8401 gccacggcga gacccgggac ggcggcgacg gcacgttcga tccgctcgac acggatgagg
     8461 tgcccgatgc cgtactgcgg gagcccgccg ccccagcggg tcaccagggt gtcgatcggc
     8521 tcggcggtga tcccggtcag ccgggccagg tcgtcgcgga ccacgcggac gagttcgtcg
     8581 tcgtcggcgt gcagcgcccc cggttcgccg aagcggccga ccgagcctcg caccagcacc
     8641 gggccttcgc cgtactgcgc ccacttgcgg gaggagaagg tgaacgcctt ggcggcgaac
     8701 ggcttcccgg ccgcgtcccg ttcccccgcg ccgatcagca cgccggacga ctccggcaac
     8761 gcggtccccg gcggaagcgc gagcgcgacc accgccatcg acgcgagttc gatcttggcg
     8821 aacaccgtcg aggccacttc cgccacacca tcgagcagcc gtcgcgccga tggcgccggg
     8881 acggcgagga gaacggagtc ggcgtcgagg tcgccgacct tccagccggt gggtgtgcgc
     8941 tcgagcgccg tcaccgtggt cccggtccgg atctcggcgc gggacagctc cgcgagccgg
     9001 gcgatcagcg tcccgaggcc gcccagcagg gtgccgaaga ccggcgccgt actcggcgag
     9061 gcggggatga gcgaggcggc gccttcggtg agcgaagtcg ctccgcgatc gatcgcggac
     9121 gcgagaccgg gcatggtcgc gcgcagcccg agcccgtccg ctcccccggc gtagaccccg
     9181 ccgagcagcg ggtcgacgag ccggtccacg agttcgtcgc cgaaccgctc gcgcagcagc
     9241 tcgccgagcg gcacgtcccc cgcgggcagt ttcagcggag acagcgaagc ctcggcctcg
     9301 accagggcgc gccccttctc cgacagcact cccgcgaccg cgtcggccga ggcgggaacg
     9361 cccatgaccg tgcccggcgg caggccgagc acggaaccac cggcgtggat cttggcgcgg
     9421 gccttggtgg ggtggacgag gctttcgccg agcccgacct cgcggaccag ggcgaccgcc
     9481 tcgggacgac gggcgaggaa ggcctccgcg ccgacgtcgt agcgctgtcc ggcgagttcg
     9541 accgtgcgga gcttcccgcc gagcgagtcc gtcgcctcga agacgacgat cctggcgtcc
     9601 tccccgagca gcgtccgcag ccggtacgcc gcggtcagtc ccgagatgcc gccaccgacg
     9661 acggcgatcg tgctcacggc tggagctcgt gcaccagccc gaccacgcgc gtcagaacgt
     9721 ccgggtcgac gcccggcagc acgccgtggc cgaggttgaa gatgtggccg tcggccgcac
     9781 ggccctcggc gtggatgcgc cggacctcgg cctcgatgac cggccaggac gcgtgcagca
     9841 gcgccgggtc gaggttgccc tggacgacac cgccgccgag ccgccggacg gcttcgtcga
     9901 gcgggatgcg ccagtcgacg ccgaccacgt cggcgcccgc gtcgcgcatg gccaccagca
     9961 gctcgccggt gccgacgccg aagtggatcc ggggcacgcc gtagcgcgcg accgcggaca
    10021 ggaccttcgc cgaatgcggc aggacgaact cgcggtagtc ccgctccgag agcgcgcccg
    10081 cccacgagtc gaacagctgg atcgcgtcgg ccccggcgtc gagctgcgcg gacaggaagg
    10141 tgatcgccat atcggcgaga cgcccggcca ggtcgtgcca cagctcgggc tcggagtgca
    10201 tgagcgcctt ggtgtgctcg tggttgcggc tcggcccgcc ctcgatgagg tacgaggcca
    10261 gggtgaacgg cgcgcccgcg aacccgatca gcggcgtctc gcccagcgac ccgaccagca
    10321 gccggacccc ttcggcgacc cgctccacct gctccggctc cagcaccggc agcgcgcgca
    10381 cgcccgcggc gtcgcggatc ggctcggcga cgaccgggcc ggtgcccgcg acgatgtcga
    10441 tgtcgagacc cgccgccttg agcgggacga cgatgtcact gaagaggatg gccgcgtcga
    10501 cgccgtgacg gcggatcggc tggagcgtga tctcggcgag catctcgggg tcgaagcagg
    10561 cctcgaacat cgaggtgccc tcgcgcagcg cccggtactc gggcagcgac cggcccgcct
    10621 ggcgcatgaa ccacacgggg gtgtgagccg ggcgttcgcc gcgcgcggcg gccaggaacg
    10681 gcgcaccagg cagggcgcga cgggccgccg ggacggtctg cgcgggagaa gcaactgaag
    10741 gagacatcac ggttcatggt gccatgtgcg ctcggaggcc ttactctcgg cgcgcctgcg
    10801 ccccgaaccg gacgat
//
