LOCUS       NZ_CP102415            21098 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Amycolatopsis roodepoortensis strain ZEL-1 chromosome, complete
            genome.
ACCESSION   NZ_CP102415
VERSION     NZ_CP102415.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis roodepoortensis
  ORGANISM  Amycolatopsis roodepoortensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Biodegradation of petroleum hydrocarbons by Amycolatopsis orientalis
            ZEL-1, genome analysis and its potential in plant protection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9489959)
  AUTHORS   Gao,T., Li,J., Ni,W., Xiong,S., Qiu,H., Li,W., Xia,W., Tao,X.,
            He,X., Yong,B., Wu,B. and Shao,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2022) College of Life Sciences, Sichuan Normal
            University, No. 1819, Section 2, Chenglong Avenue, Longquan
            District, Chengdu 610101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON v. 0.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024628825.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 06:13:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,064
            CDSs (total)                      :: 8,994
            Genes (coding)                    :: 8,928
            CDSs (with protein)               :: 8,928
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 27 of 66
            Pseudo Genes (incomplete)         :: 44 of 66
            Pseudo Genes (internal stop)      :: 4 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:48:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 58049
            Orig. end                         :: 79147
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102415.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21098
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RRE-containing"
                     /region_number="2"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..21098
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21098
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11098](+)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11098
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     misc_feature    26..40
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            351..2393
                     /locus_tag="NQK81_RS00280"
                     /old_locus_tag="NQK81_00280"
     CDS             351..2393
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029608.1"
                     /locus_tag="NQK81_RS00280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00280"
                     /product="M3 family metallopeptidase"
                     /protein_id="WP_257486469.1"
                     /transl_table=11
                     /translation="MISQDNPFAAPSELPYALPPFDRIADEHFLPAFEAGLAEHAAEIE
                     EIAGSAEPPTFENTIVALEKSGRLLSRVSSVFFNLAGSNANDEIQRVQAEMAPRLAAHA
                     DAINLNPALFARINSLFERRDELGLDAESLRLLERHHTDVRRAGAGLPEADQKRLRELN
                     ERISTLSTRFQQNLLKDTNELAVVVDDVAELAGLGEDEIATAAETAGEEGKYVLKLTLP
                     TAQAALASLEDRALRERIFTASVSRGNRGNEFDNNEVVAQLVRLRAERAAVLGFPDHAS
                     YVIEDETAKTAEAAVGLLERLAPVAVANAKTEAEELQRYLEADIPGAVLRPWDWAFYAE
                     RVRKDRFEVDTAALRPYFELNRVLTDGVFFAASKLYGLSFTEREDLPKYHPEVRIFEVF
                     DADGAELGLFLLDYYARDSKRGGAWMNNFVDQTRLLGTKTVVVNVLNVAKPPAGEPTLL
                     TFDEVVTAFHEFGHALHSLLSDVEYPMFSGTNVPRDFVEYPSQVNEMWMLWPEVLANYA
                     KHHVTGEPLPQEQIDKLLASQQYGEGFSTTEYLAASLLDQAWHALGTEDNVEDVQRFEA
                     EALTKAGVALEAVPPRYRTTYFAHIFSGGYSAGYYSYIWSEVLDADTVQWFRENGGLTR
                     ENGDHFRRELLGRGGSVDPMAAFRAFRGRDPEIEPLLKRRGLAGA"
     gene            complement(2442..3665)
                     /locus_tag="NQK81_RS00285"
                     /old_locus_tag="NQK81_00285"
     CDS             complement(2442..3665)
                     /NRPS_PKS="Domain: Aminotran_5 (32-397). E-value: 9.2e-57.
                     Score: 184.5. Matches aSDomain:
                     nrpspksdomains_NQK81_RS00285_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635667.1"
                     /locus_tag="NQK81_RS00285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00285"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_257486470.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.6e-49, bitscore:
                     165.6, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 4.3e-06,
                     bitscore: 25.2, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLHDSATGNRAESLLDVPALRADTPGSEKVVHFNNAGCGLLAKP
                     VLAAMLDHLNLEAEIGGYEAAAARAAEVTGFYDEIAALINARPRNIAFAASATHAYSTA
                     LSSIRFEPGDVVLTTRNDFISNQIAFLSLRKRFGVDVVHAPDSREGGVDVEAMAALMRT
                     KKPKLVAATHIPTNSGLVQPVAEIGRHCRDLGLLYLVDACQSIGQYPVDVEEIGADLLT
                     STCRKFLRGPRGSGFLYVSDRFLDAGHEPLFIDMHGARWSAPGAYEPAKSASRFEEWEF
                     PYATVLGCAAAARYAREVGLEAISRRTPALAARLRDGLGAIPGVEVLDRGPELGALVTF
                     SIKGWEAVPFKEAMDARRINSALSYREFAQFDFGDKDVDWCLRLSPHYYNTEREVDDVA
                     AAVAELAA"
     aSDomain        complement(2475..3569)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS00285_Aminotran_5.1"
                     /evalue="9.20E-57"
                     /label="NQK81_RS00285_Aminotran_5.1"
                     /locus_tag="NQK81_RS00285"
                     /protein_end="397"
                     /protein_start="32"
                     /score="184.5"
                     /tool="antismash"
                     /translation="HFNNAGCGLLAKPVLAAMLDHLNLEAEIGGYEAAAARAAEVTGFY
                     DEIAALINARPRNIAFAASATHAYSTALSSIRFEPGDVVLTTRNDFISNQIAFLSLRKR
                     FGVDVVHAPDSREGGVDVEAMAALMRTKKPKLVAATHIPTNSGLVQPVAEIGRHCRDLG
                     LLYLVDACQSIGQYPVDVEEIGADLLTSTCRKFLRGPRGSGFLYVSDRFLDAGHEPLFI
                     DMHGARWSAPGAYEPAKSASRFEEWEFPYATVLGCAAAARYAREVGLEAISRRTPALAA
                     RLRDGLGAIPGVEVLDRGPELGALVTFSIKGWEAVPFKEAMDARRINSALSYREFAQFD
                     FGDKDVDWCLRLSPHYYNTEREVDD"
     gene            3664..5046
                     /locus_tag="NQK81_RS00290"
                     /old_locus_tag="NQK81_00290"
     CDS             3664..5046
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (161-456). E-value:
                     7.1e-26. Score: 83.1. Matches aSDomain:
                     nrpspksdomains_NQK81_RS00290_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635666.1"
                     /locus_tag="NQK81_RS00290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00290"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_257486471.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1e-23, bitscore:
                     83.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSSTSALVAQLRSLVRSAAAGERLPSSRELIERYRVGPGTVSR
                     AIALLAAEGTVVTRPGSGTYVATKPAAAGEPLDTAWQTVALTDRSVDTRSLTGVLGPPP
                     EGAILLDGGYPHRSLQATRFLSAALARAGRRPDAWDRAPAAGLAALRSVFAGPAGASPD
                     DVLITAGGQSGLSMAFRAIAAPGSPVLVESPTYPGALAAARAAGLRPVPVPIDDDGVRP
                     DLLAEAFAMTGARLFYCQPAFHNPTGTVLAAERRTRVLDVARAAGAFVLEDDFARYLSH
                     GAAAPRPLITDDRDGTVVHLTSVTKAAAPSLRIGALIARGPVLDRMKAIRRVDDFFVSR
                     PLQEAALELLSAPSWERHVRRLGSELRERCVKAAAALAGKCPEWTIARLPEGGLHLWVR
                     LPPGSDANALARVARERGVVVGAGERFFATEPSGPFLRLNFAAAADIAELTEGIRRLDP
                     NV"
     aSDomain        4147..5031
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS00290_Aminotran_1_2.1"
                     /evalue="7.10E-26"
                     /label="NQK81_RS00290_Aminotran_1_2.1"
                     /locus_tag="NQK81_RS00290"
                     /protein_end="456"
                     /protein_start="161"
                     /score="83.1"
                     /tool="antismash"
                     /translation="PDDVLITAGGQSGLSMAFRAIAAPGSPVLVESPTYPGALAAARAA
                     GLRPVPVPIDDDGVRPDLLAEAFAMTGARLFYCQPAFHNPTGTVLAAERRTRVLDVARA
                     AGAFVLEDDFARYLSHGAAAPRPLITDDRDGTVVHLTSVTKAAAPSLRIGALIARGPVL
                     DRMKAIRRVDDFFVSRPLQEAALELLSAPSWERHVRRLGSELRERCVKAAAALAGKCPE
                     WTIARLPEGGLHLWVRLPPGSDANALARVARERGVVVGAGERFFATEPSGPFLRLNFAA
                     AADIAELTEGIRRL"
     gene            complement(5065..5694)
                     /locus_tag="NQK81_RS00295"
                     /old_locus_tag="NQK81_00295"
     CDS             complement(5065..5694)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334437.1"
                     /locus_tag="NQK81_RS00295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00295"
                     /product="hypothetical protein"
                     /protein_id="WP_257486472.1"
                     /transl_table=11
                     /translation="MPTQATEAPGTRRRLARIATEVLAPWVWVLGLPLVVAWKVTGHHL
                     GETVLWGLVVGVTGSLIPMAVIVRGARKGKWDSHHVTDRARRLVPFAVCIGSLAAGFVI
                     LVAGGSPHEMIALAAAELVCLVAALAITFGLRFKISMHAMVAAGAAVMLIRVYGPWLAL
                     LFLGVGWVCWSRVELGDHTKAEVTAGAAAGIVLGGGVYLLLEAVLA"
     gene            complement(5765..7564)
                     /locus_tag="NQK81_RS00300"
                     /old_locus_tag="NQK81_00300"
     CDS             complement(5765..7564)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334436.1"
                     /locus_tag="NQK81_RS00300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00300"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_257486473.1"
                     /transl_table=11
                     /translation="MPEIRFFVSPEGRDDWAGTADRPFGTPKAAQEAVRAVTAGMTGDV
                     IVTFGTGVHHLEAPLVFDDALGDSGSNGFRVIYQADGYGTPEQAKVELSGGREITGWTT
                     GRDGIWTAPIGALAPRRLTVDGRPAVRAARTEGLPGTVTKTATGYVTDSTEPQSWPDPS
                     GLEFVYPGVYPWSEARLAVASITGDATSTTVTMGQPAFGHAKSLYDSKWYGAGGDGTWD
                     GLAAPSVLENNVAFLTEPGTFAVSRTEGDGHVLHYRPRPGEEIGKTRVVAPMLESLITG
                     QGVHDLVLRGFTFADATWTGVARTGGYLHYHGNARYERGEFQKIDLGAEVGYVHVPAEP
                     VQLPANVSLTSANRVTLEGNHFTRLGAGALLIEGGADVGIRGNVFDEISGSGIAVHAGT
                     RVSIEDNLVHHIGTEYRGSPAVLVVESEETTVAHNEIHAVPHNGIVITGGEKAKATQVL
                     SNLVERSMGKLMDGGGIYLSAPQGSSFAGGALVRGNVIRDVLTSYNFGLYADYGAAWIT
                     VLGNIVCRADTPIVLQVGPPMENVAFVGNFWDVLPDGYDTPPETVAVAGNTVLPKEGFD
                     EAVRADPAAADILARAGRRGLER"
     gene            7654..8523
                     /locus_tag="NQK81_RS00305"
                     /old_locus_tag="NQK81_00305"
     CDS             7654..8523
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635663.1"
                     /locus_tag="NQK81_RS00305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00305"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_257486474.1"
                     /transl_table=11
                     /translation="MAEDDAQPDPERAIELLWGTPEPERPGLSLGRIVAAAVEVADAEG
                     LAALSMRKVAERFGTTTMSLYRYVPGKAELVELMRDAVYGRFPMEPSDGLGWRAGLERW
                     ARRAWEMHRRHPWLVDSAGSRRLPGPNIMAGYDHALDVASRSGLAPAQVIAVVNLVGGF
                     VESVARQAGETAELQRRTGVSHERWWSERESLFERMDAYPALERLWKDGGMDAPLDPFE
                     FGLQRTLDGVQALVESRGREVMRDETERGECVVCGKELDGGGRGRPRDYCSRACQQRAY
                     RRRQAETS"
     gene            complement(8572..8805)
                     /locus_tag="NQK81_RS00310"
                     /old_locus_tag="NQK81_00310"
     CDS             complement(8572..8805)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164761.1"
                     /locus_tag="NQK81_RS00310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00310"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_126733114.1"
                     /transl_table=11
                     /translation="MNNDSSRPTLLGLSGWWKSSYSAGANGCVEVNDSVKGYVGVRDSK
                     LGENSPVLVFEPHEIKAFVLGAKNGEFDHLLS"
     gene            complement(8795..9664)
                     /locus_tag="NQK81_RS00315"
                     /old_locus_tag="NQK81_00315"
     CDS             complement(8795..9664)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164759.1"
                     /locus_tag="NQK81_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00315"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_257486475.1"
                     /transl_table=11
                     /translation="MGTLTPTAIKRWIALEMKRLREAAGYDRTAAAERIGKATTVIAHI
                     ETSRNLPAPADLEVLLDFYGVPERAPFFRDLTKRAKRGRDWWINFDHGVFDWFNLYLGL
                     ETGAARISTVDLVVLPGLFQTRDYALDIMREGRRWNNKAELETMVDLRMARQEILDRPG
                     DAPHIWSILDESVLRRQVGGPAVMRDQLEHLIELSERPNIDIQVLSFTAGAHGASDGGF
                     ILLDYPSEFGGDPGTVYVENRREGLYYEDTEDLRRFRSTFERLQAQAEHPGRTRSFIQN
                     LARELNEQ"
     misc_feature    complement(9691..9704)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.91"
                     /tool="antismash"
     aSDomain        10001..10270
                     /aSDomain="Pantocin_Microcin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing E1-like protein in a
                     pantocin/microcin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_NQK81_RS00320_Pantocin_Microcin_RRE.1
                     "
                     /evalue="8.80E-22"
                     /identifier="RREFam017.1"
                     /label="NQK81_RS00320"
                     /locus_tag="NQK81_RS00320"
                     /protein_end="90"
                     /protein_start="0"
                     /score="68.1"
                     /tool="antismash"
                     /translation="MRPRIKDEHRPVRFGDGWIRIGGEVFGIAAELRDSDGAVWALLEL
                     LDGTRTVSQVVTDLARLVPGTRPDEVTAAIEKLAELGYVENADEP"
     gene            10001..11098
                     /locus_tag="NQK81_RS00320"
                     /old_locus_tag="NQK81_00320"
     CDS             10001..11098
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635656.1"
                     /locus_tag="NQK81_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00320"
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_257486476.1"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 2.8e-19,
                     bitscore: 68.1, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="ThiF (E-value: 2.1e-46, bitscore: 157.3,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPRIKDEHRPVRFGDGWIRIGGEVFGIAAELRDSDGAVWALLEL
                     LDGTRTVSQVVTDLARLVPGTRPDEVTAAIEKLAELGYVENADEPTCTDLTERQLERHS
                     RSRSLYRWIDLTRRTSSWDFQVLLARARVVLVGVGGVGCTAALGLVMSGVGRLHCVEPD
                     VVELSNLGRQILYTEEHLGLPKVDVAVERLRAANSDVEVTGEPTRIGNAASLRRLAADC
                     DVLIMTADHPPEIRTWTNQACASTGTPWVYGGYHGPQVNLGVYRPGAGPCYECARLAER
                     DRDAARPPLATWRPAASLPAHAANAVTAGVAGNLVAHAVMRLITNAPKLALNCQYGFSL
                     ATMDHSFVTSLERPHAECRTCLAAG"
     misc_feature    10124..10126
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11104..12030)
                     /locus_tag="NQK81_RS00325"
                     /old_locus_tag="NQK81_00325"
     CDS             complement(11104..12030)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164754.1"
                     /locus_tag="NQK81_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00325"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_257486477.1"
                     /transl_table=11
                     /translation="MIAQRAGAHFASTPRKKIMPTSSPHPGSDRVRLATKLRELRAATG
                     LSGNQFAKNLGWPQSRVSKIETAVQFPTGDDIAGWLAAAGAADQEDSVLELLKRARVES
                     VSFRDEFRKPGGAAAKQRSVLELEQRASRVATFQPALLPGPVQTPGFMRELLALPSGPA
                     RTGGAADADIERMVAVRIERQAQLHDPGKAMPLVIGEAALWIRVGDLDTQLGQLDRLSS
                     LADLRTVDLRILPFSKAMPIAPLHGFRIYDAELVVVELFAGEHLIRDPEEIALYEHMFG
                     TLHAEALSGDAAARLIRSVSRRLTEDD"
     gene            12565..13698
                     /locus_tag="NQK81_RS00330"
                     /old_locus_tag="NQK81_00330"
     CDS             12565..13698
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164753.1"
                     /locus_tag="NQK81_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00330"
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_257486478.1"
                     /transl_table=11
                     /translation="MITEVLLEQPEEDIRTPVLFKGFVEPGAQAAQALLVLGKVARTRF
                     WMPPSMVNRLIAESDPVFTADRTTLRVEAFSPCCGVYARLDLLDVGDLGEGCTNVDLSD
                     ATRTVLAGVVAQDPLHLTVRGEGIRLRTLDGRNDESVVPLPDRWVAGFAEVAAVTEGAV
                     ERAKLDKAETRNFLRRLPTAVGRSWAVASGRGLRLTSRPGRDSIPATGLHRLRVIEPLV
                     RFAQGLTVYTRPDSEVATWVLQLDRARLVVTLSPEPSRGFSGEGGWLNSLAAGLEAPDL
                     VAGRAGHDVVDGTWFPRELPSGNAPANGRLAKARELVAAGAVRLLPDGGYEITRAGSVQ
                     RVRLSPAGCTCAWWAKHQETRGPCSHVLAARMSSAES"
     misc_feature    12580..12582
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(13681..16131)
                     /locus_tag="NQK81_RS00335"
                     /old_locus_tag="NQK81_00335"
     CDS             complement(13681..16131)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164751.1"
                     /locus_tag="NQK81_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00335"
                     /product="DUF6493 family protein"
                     /protein_id="WP_257486479.1"
                     /transl_table=11
                     /translation="MQLNALLTGQPSEVLTRVLALTRAERKALVPELLAAFKDSSEFIE
                     IKPNTYGVRNPLRGQQAATALWAALPASDPLLPKLWIASPTILERVLQDRPLAERKTIA
                     LRVIGHQTAWEAVRRMILDGDLKPVRTPEYHAGWFRWAGRFGGKLLAERLRTEPEALTE
                     LWELLTIEGNGDASFAAFDKYVRDQDKWSTALLELTKTGEVDRTRLLDLTLDILARDFA
                     AFRAQWYAALHEALAPTPEERTKAQHRYARLCGSGLPRTVSFAVKALSLVDKQGTLDDE
                     VALQHLPAAAAGRGATTAERALKLAGRIADRRPELSDLADEVFEAALTHEASDVRALAI
                     ARLGVTPAQAPAIETAEYPEPTRPPWDQRKTLTIPLDIPDTPAAVAEALSSLLADPDQA
                     DLLLAALDGAHRVADELPDALKPLVKRAARIDAQRYSPAVPKMIATLVLGLTGHDVPPP
                     PKVGDWRRPLHHRITALLEGTAAPVSAATHQGGWLDPVVFVSRMLEHPDAAEDDVVDAL
                     LRLAPDTRPEALALASELTGPHADNIRYALGGPAGRKTGWIALAAARARYPESADPAAA
                     LRGEPGPPLTWTAKAGPSGRHNLTTLDIEPAPEAASPKVPWLGVPFDGPATEWLGFTEC
                     HEAVASPYDRDHIEAAVAGYLFFNDTEFGNPVEKAALPPFLDSPSVPGFPGTMLLATTL
                     TLKRSTAVQLGTDAVLTLLDRRMLGPRALGAALGVLGPYLIAPRLASRLSIIANEHPVA
                     VLSTLDTLLPELEHARRGIFALLELAADLVERGAGTISDETKEWLARFKGSSKAAKAAS
                     RLRR"
     gene            complement(16361..17386)
                     /locus_tag="NQK81_RS00340"
                     /old_locus_tag="NQK81_00340"
     CDS             complement(16361..17386)
                     /NRPS_PKS="Domain: PKS_ER (9-262). E-value: 8.9e-11. Score:
                     33.4. Matches aSDomain:
                     nrpspksdomains_NQK81_RS00340_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659645.1"
                     /locus_tag="NQK81_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00340"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_257486480.1"
                     /sec_met_domain="ADH_N (E-value: 4.3e-32, bitscore: 109.3,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 5.2e-19, bitscore:
                     67.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAIQVTGYHEKLQLTEVAEPEPTGPYDVIVRIGGAGVCRTDLHI
                     LEGQWAEKSGVTLPYTIGHENAGWVHAVGSAVTNVTEGDKVIVHPLITCGLCRACRSGD
                     DVHCAQSQFPGIDTAGGYAEYLKTSARSVVRIDDTLEPADVAALADAGLTAYHAAAKAA
                     RRLRPGDRCVVIGAGGLGHIGIQVLKALTAAELVVVDRNPDAVELAVSIGADHGVIADG
                     TQIDQVLDLTGGEGAETVIDFVGEGGATKDGLAMLRRAGDYHVVGYGENIDVPTIDVIS
                     TEINLIGNLVGSYTDLCELMVLAAQGRVKLHTARYPLEEFQTALDDLDAGRIRGRAILV
                     P"
     aSDomain        complement(16601..17359)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NQK81_RS00340_PKS_ER.1"
                     /evalue="8.90E-11"
                     /label="NQK81_RS00340_PKS_ER.1"
                     /locus_tag="NQK81_RS00340"
                     /protein_end="262"
                     /protein_start="9"
                     /score="33.4"
                     /tool="antismash"
                     /translation="HEKLQLTEVAEPEPTGPYDVIVRIGGAGVCRTDLHILEGQWAEKS
                     GVTLPYTIGHENAGWVHAVGSAVTNVTEGDKVIVHPLITCGLCRACRSGDDVHCAQSQF
                     PGIDTAGGYAEYLKTSARSVVRIDDTLEPADVAALADAGLTAYHAAAKAARRLRPGDRC
                     VVIGAGGLGHIGIQVLKALTAAELVVVDRNPDAVELAVSIGADHGVIADGTQIDQVLDL
                     TGGEGAETVIDFVGEGGATKDGLAMLRRAGD"
     gene            complement(17383..18099)
                     /locus_tag="NQK81_RS00345"
                     /old_locus_tag="NQK81_00345"
     CDS             complement(17383..18099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234354.1"
                     /locus_tag="NQK81_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00345"
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="WP_257486481.1"
                     /transl_table=11
                     /translation="MREHDAYTALHAVYDPELDEPITDLGFVRSFTVDGTEVTVHLRLP
                     TSFCSPNFAYLMASDAKDVLTALDGVSRVHVVLDDHHDSDLINRGLAADAGYRGTFGDE
                     ADQDLDELRLTFRRKAHTAAMERALTRLLRENRELTEDRLHDITLADLPDHRTTHALLR
                     RRAALGLDTAPEAPVLVDDHGTPYPREEIPLRLRFARSVRISIDGNAHFCRGLLRTRYP
                     ESAADQTPRNQEVHAS"
     gene            complement(18101..19114)
                     /locus_tag="NQK81_RS00350"
                     /old_locus_tag="NQK81_00350"
     CDS             complement(18101..19114)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416142.1"
                     /locus_tag="NQK81_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00350"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_257486482.1"
                     /transl_table=11
                     /translation="MYTKDGESYFIVDAHVALWDARPENQANIHGKQFIDCFYDYHRNL
                     SPESELWSYEEYLYYGGERLMKDLFQDGYVDHAIFQPAHLGAFYNNGFGQTEEAFALTK
                     KHPGKLTYNHCFDPRLEQAGLDQLRRDAERFGLKGVKLYTAEWRDDSRGYKLDDPWCRR
                     YLETCLDLGIRNIHIHKGPTIRPLDRDAFDVADVDKVATDYTDLNFVVEHCGLPRLEDF
                     CWIATQEPNVHAGLAVAMPFIHTRPRYFAQIIGELLYWLDEDRIQFSSDYALWTPKWLV
                     ERFVDFQIPEDMTEYAPLTNDQKRKILGLNAAAMYDLPVPEELQVRRLETQAVSVA"
     misc_feature    19152..19163
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    19199..19213
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.98"
                     /tool="antismash"
     gene            19295..20731
                     /locus_tag="NQK81_RS00355"
                     /old_locus_tag="NQK81_00355"
     CDS             19295..20731
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678008.1"
                     /locus_tag="NQK81_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NQK81_00355"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_257486483.1"
                     /transl_table=11
                     /translation="MESANAVIAPRLRASWQRSARYGAPEDEVRPVFTGSLDTGSLLYE
                     CGNEVLRGLQATLANEPVSLMITDSEGLVLSRLCTDASIVRSLDRVHLAPGFYFAESNA
                     GTNGLGLALADRAPTLVRAAEHYCTGLRGYTCAAVPVLDPLTGALAGSVNLTTWSDQSS
                     ELLLALAQAAAGNTAALMLARASGRTPRPAPRGEVFRVHSDRFDPADVPSLSPGWSDAL
                     GEVGSALSRGRVVAVVGEAGAGKTALASAARRATRPRDRVLNARPPAPDDVDTWLTLWT
                     PELEKDGTCVIVSGVDTLPAWAAAELAGLFADARREGEPPPFVLTAEDAGAIPEALAPL
                     VEVVVEVPALRFRPDDIVPLAHHFARRDRGREVAFTPAAVRALTACDWPENAKQLRRVV
                     REAASRSDVIDSRHLPAEVFSGVGHRLSRLQAMERDEIVRCLAEPGATVVGAAEKLGMG
                     RATLYRKMGQYGIKARQFRS"
     misc_feature    21079..21088
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
ORIGIN
        1 gcgatgtgat caaggcgatc ctcgccgacg cgctcggcca gcacctcgat tccttccagc
       61 ggatcgtggt cgacccggct tcggtctcgg tcgtccggta caccgagact cgtccgttcg
      121 tgttgcgcgt caacgacaac ggcggcgacc tggccggtat cgccccaccc gaacccccga
      181 ccaaagccaa gcgcggcaag ggaaaacgca gctccgacgc ggtggtgggc ggtaccaccg
      241 gagcgtgacc acttccgctg tggcgtcacc gccacggacg gcatccaccg gcccctcgct
      301 gcccgtaagg tggccagacc agcagtttcg acgacccggg agcaacaccg atgatttcgc
      361 aggacaaccc gttcgccgcg ccgagtgagc tgccctacgc ccttccgccg ttcgaccgga
      421 tcgccgacga gcacttcctt cccgccttcg aagcgggcct ggccgagcac gccgcggaaa
      481 tcgaggagat cgccggttcg gccgagccgc cgaccttcga gaacaccatc gtcgcgctcg
      541 aaaagtccgg caggctgctg tcccgggtgt ccagcgtgtt cttcaacctc gccgggtcga
      601 acgcgaacga cgagatccag cgcgtgcagg ccgagatggc gccgcggctg gccgcgcacg
      661 ccgacgccat caacctgaac ccggcgctgt tcgcccggat caactccctg ttcgagcgcc
      721 gggacgaact cggcctcgac gccgagtcgc tgcgtctgct ggagcgtcac cacaccgacg
      781 tgcgccgtgc cggcgccggt cttccggaag ccgaccagaa gcgcctgcgc gagctgaacg
      841 agcggatttc gacgctgtcg acccggttcc agcagaacct gctcaaggac accaacgaac
      901 tggccgtggt cgtcgacgac gtcgccgaac tcgcgggact cggtgaggac gagatcgcca
      961 cggcggccga aaccgccggc gaggagggca agtacgtcct caagctgacc ctgccgaccg
     1021 cgcaggccgc gttggcgtcg ctggaagacc gcgcgctgcg cgagcggatc ttcaccgcgt
     1081 cggtctcccg tggcaaccgg ggcaacgagt tcgacaacaa cgaggtcgtg gcccagctgg
     1141 tccggttgcg tgccgagcgg gccgccgtgc tcggcttccc cgaccacgcc tcgtacgtca
     1201 tcgaggacga gaccgcgaag accgccgagg ccgccgtcgg tctcctggag cggctcgcgc
     1261 ccgtcgcggt cgccaacgcc aagacggagg ccgaagaact gcagcggtac ctcgaagccg
     1321 acatccccgg cgcggtcctg cggccgtggg actgggcgtt ctacgccgaa cgcgtccgca
     1381 aggaccggtt cgaggtcgac accgccgcgc tgcgcccgta cttcgagctg aaccgggtgc
     1441 tgaccgacgg cgtcttcttc gccgcgtcca agctgtacgg cctgagcttc accgagcggg
     1501 aagacctgcc gaagtaccac cccgaggtcc ggatcttcga ggtcttcgac gccgacggcg
     1561 cggaactcgg cctgttcctg ctcgactact acgcccggga ctcgaagcgc ggcggcgcct
     1621 ggatgaacaa cttcgtcgac cagacgaggc tgctgggtac caagacggtc gtggtgaacg
     1681 tgctcaacgt cgccaagccg ccggcggggg agccgaccct gctgaccttc gacgaggtcg
     1741 tcaccgcgtt ccacgagttc gggcacgcgc tgcactcgct gctttcggac gtcgagtacc
     1801 cgatgttctc gggcaccaac gtcccgcgtg acttcgtcga gtacccgtcg caggtcaacg
     1861 agatgtggat gctctggccc gaggtgctgg ccaactacgc caagcaccac gtgaccggtg
     1921 agccgctgcc gcaggagcag atcgacaagc tccttgcgtc gcagcagtac ggcgaaggct
     1981 tctcgaccac cgagtacctc gcggcgtccc tcctcgacca ggcatggcac gcgctgggca
     2041 ccgaggacaa cgtcgaggac gtccagcggt tcgaggccga ggcgctgacc aaggccggtg
     2101 tcgcgctgga agccgtgccg ccgcgctatc gcaccaccta cttcgcgcac atcttcagcg
     2161 gcggctacag cgccgggtac tactcctaca tctggagcga ggtcctcgac gccgacaccg
     2221 tccagtggtt ccgggagaac ggcgggctca ccagggagaa cggcgaccac ttccgccgcg
     2281 aactcctcgg ccggggcggc agcgtcgacc cgatggccgc cttccgcgcg ttccgtggcc
     2341 gcgacccgga gatcgagccg ctcctgaagc ggcgcggcct cgcgggcgcc tgaccgttcc
     2401 gccgcctggg cacccccagt gtccaggcgg cggaaccgcc ctcaggcggc cagttcggcg
     2461 acggccgcgg ccacgtcgtc cacctcccgt tcggtgttgt agtagtgcgg gctcaaccgc
     2521 aggcaccagt cgacgtcctt gtccccgaag tcgaactgcg cgaactcgcg gtagctgagc
     2581 gcggagttga tccggcgggc gtccatcgcc tccttgaagg gcaccgcttc ccagcccttg
     2641 atggagaacg tcaccagcgc gcccagctcc gggccccggt cgagcacttc gacgccgggg
     2701 atggcgccga gcccgtcccg cagtctcgcc gcgagcgcgg gggtgcgccg tgagatcgcc
     2761 tccaggccga cctcacgggc gtacctcgcg gcggcggcgc atccgagcac ggtggcgtag
     2821 gggaattccc attcttcgaa ccgggaagcg gacttcgccg gttcgtacgc gccgggcgcg
     2881 ctccagcggg ctccgtgcat gtcgatgaac agcggctcgt gcccggcgtc gaggaagcgg
     2941 tcggagacgt acaggaaccc cgaaccacgt ggcccgcgca ggaatttgcg gcacgtcgag
     3001 gtgagcaggt cggcgccgat ctcctcgacg tcgacggggt actggccgat cgactggcag
     3061 gcgtcgacca ggtagagcag gccgaggtcc cggcagtgcc gcccgatctc ggcgaccggc
     3121 tggacgaggc cggagttggt cgggatatgc gtggcggcga ccagcttcgg cttcttggtg
     3181 cgcatcaggg ccgccatcgc ttcgacgtcg acaccgccct cccgcgagtc cggcgcgtgc
     3241 acgacgtcga ctccgaagcg cttccgcagc gacaggaacg cgatctggtt ggagatgaag
     3301 tcgttgcggg tggtgaggac gacgtcgccg ggctcgaacc ggatcgacga caacgccgtg
     3361 gagtaggcgt gggtggcgct cgcggcgaag gcgatgttgc gaggccgcgc gttgatgagc
     3421 gcggcgatct cgtcgtagaa accggtgacc tcggccgcgc gggccgcggc ggcctcgtat
     3481 ccgccgatct cggcttcgag gttcagatga tcgagcatcg cggccaggac cggcttcgcc
     3541 agaagcccgc aaccggcgtt gttgaagtgg acgaccttct cgctgcccgg cgtatcggct
     3601 cgcagggctg ggacgtccag tagtgattcc gcccgatttc cagtagcact atcatgtagc
     3661 ttcatgtcgg atagtagcac ttccgcgctc gtcgcccagc tgcgttccct cgtccgctcg
     3721 gcggccgcgg gggagaggtt gcccagcagc cgggagctga tcgagcgcta tcgggtcggt
     3781 ccgggaacgg tctctcgcgc gatcgccctg ctggccgccg agggcacggt cgtgacccgt
     3841 cccgggagcg gaacgtacgt cgcgacgaag ccggcggccg cgggcgaacc gctcgacacg
     3901 gcatggcaaa cggtcgcgct gaccgaccgc agcgtcgaca ccagatccct caccggcgtc
     3961 ctcggcccgc caccggaggg cgcgatcctg ctcgacggcg gctacccgca tcggtcgctg
     4021 caggcgactc gcttcttgag cgcggcactg gcgcgggcgg gccgccgccc ggacgcctgg
     4081 gatcgcgcac ccgccgccgg tctggccgcg ctgaggtcgg tcttcgccgg cccggccggt
     4141 gcgtcaccgg acgacgtcct gatcaccgcc ggcgggcaga gcgggctgtc catggcgttc
     4201 cgtgccatcg cggcgcctgg cagcccggtc ctcgtcgaat cgccgacgta cccgggggcg
     4261 ctcgccgccg cgcgggcggc ggggctccgg ccggtgccgg tcccgatcga cgacgacggg
     4321 gtccgcccgg acctgctggc cgaggcgttc gcgatgaccg gagcccggtt gttctactgc
     4381 cagccggcct ttcacaaccc gaccgggacg gtgcttgcgg ccgaacggcg cacgcgggtg
     4441 ctggacgtcg cccgggcggc gggcgcgttc gtcctggagg acgacttcgc ccggtacctc
     4501 tcccacgggg ccgccgcgcc acgtccgctg atcaccgacg accgggacgg cacggtcgtc
     4561 catctgacgt cggtgaccaa ggccgccgcg ccgagcctcc ggatcggcgc gctgatcgcg
     4621 cgagggccgg tgctcgaccg gatgaaggcg atccggcggg tcgacgactt cttcgtctcc
     4681 cgcccactgc aggaggccgc cctcgaattg ctcagcgcgc cttcgtggga acggcacgtt
     4741 cgaaggctcg gttcagaact gcgggaacgg tgcgtgaaag cggccgcggc gctggcgggg
     4801 aagtgtcctg agtggacgat cgcgcggctc cccgagggcg gtctgcacct gtgggtgcgg
     4861 ctgccgccgg ggagcgatgc gaacgcgctc gctcgcgtgg cgagagagcg aggggtcgtg
     4921 gtcggggcag gggagaggtt cttcgccacc gagccgtccg gaccgttcct ccggctgaac
     4981 ttcgcggccg ccgccgacat cgccgaactc accgaaggca tccggcgcct cgacccgaat
     5041 gtataacgac ctatacggcg tggctcaggc caaaaccgct tcgagaagca ggtaaacacc
     5101 accgccaagg acgatgccgg cggccgctcc agccgtgacc tcggccttgg tgtgatcgcc
     5161 gagttcgacg cgggaccagc agacccagcc gacgccgagg aagaggagcg cgagccacgg
     5221 gccgtagacg cggatgagca tcacggccgc gcccgcggcc accatcgcgt gcatcgagat
     5281 cttgaacctc agcccgaagg tgatcgcgag cgcggcgacc aggcagacga gttcggcggc
     5341 cgcgagcgcg atcatctcgt gcggggaacc gccggcgacg aggatgacga atccggcggc
     5401 cagtgagccg atgcagaccg cgaacgggac gagccgtctg gcgcgatccg tgacgtggtg
     5461 actgtcccac ttgcctttcc gggcgccgcg cacgatcacg gccatcggga tcagcgagcc
     5521 ggtcacgccg acgacgagtc cccacagcac ggtctcgccg aggtggtggc cggtgacctt
     5581 ccaggcgacg accagcggca gaccgagcac ccagacccag ggggcgagca cctcggtcgc
     5641 gatccgcgcg aggcgccggc gggtcccggg ggcttccgtt gcctgggtgg gcatcgcgcg
     5701 tgttctcctc gtgaatcgtg gccggatcgg ctcctgccgg actcgcgccc ggcaggagcg
     5761 gtgatcatcg ttccagaccg cggcgccccg ctcgtgcgag gatgtcggcg gcggccggat
     5821 cggcgcggac ggcctcgtcg aagccttcct tcggcagcac ggtgttcccg gccacggcca
     5881 ccgtctcggg cggggtgtcg tagccgtcgg gcaggacgtc ccagaagttg ccgacgaagg
     5941 cgacgttctc catcggcggg cccacctgga gcacgatcgg ggtgtcggcc cggcagacga
     6001 tgttgcccag cacggtgatc cacgccgcgc cgtagtcggc gtacagcccg aagttgtacg
     6061 aggtgaggac gtcgcggatc acgttcccgc ggaccagcgc cccgcccgcg aacgacgagc
     6121 cctgtggggc cgagaggtag atcccgccgc cgtccatcag cttccccatg ctgcgttcga
     6181 cgagattcga gagcacctgg gtggccttgg ccttctcgcc gccggtgatc acgatgccgt
     6241 tgtgcgggac ggcgtggatc tcgttgtggg ccacggtggt ctcttcggac tcgacgacga
     6301 ggacggcggg ggagccgcgg tactcggtgc cgatgtggtg gacgaggttg tcctcgatgg
     6361 agaccctggt accggcgtgg acggcgatcc cactgccgga gatctcgtcg aagacgttcc
     6421 cccggatgcc gacgtcggcg ccaccctcga tcaggagcgc tcccgcaccg agccgggtga
     6481 agtggttgcc ctccaaggtg acccggttcg ccgacgtcag gctgacgttg gcgggcaact
     6541 ggaccggctc ggcaggcaca tggacgtagc ccacttcggc gccgaggtcg atcttctgga
     6601 actcgccccg ctcgtagcgt gcgttgccgt gatagtgcag gtaaccgccg gtgcgggcaa
     6661 cgccggtcca ggtcgcgtcg gcgaaggtga agccacgcag tacgaggtcg tgcacgcctt
     6721 gtccggtgat gagcgactcg agcatcgggg cgacgaccct ggtcttgccg atctcctcgc
     6781 cggggcgggg acggtagtgc aggacgtggc cgtccccttc ggtgcggctg acggcgaagg
     6841 tgcccggttc ggtcaggaag gcgacgttgt tctcgagcac ggaaggcgcg gcgagcccgt
     6901 cccaggtgcc gtctccgccc gcgccgtacc acttggagtc gtagagggat ttggcgtggc
     6961 cgaaagcggg ctggcccatc gtgacggtgg tggaggtggc gtcgccggtg atcgacgcga
     7021 ccgcgaggcg ggcttcggac cacgggtaga cgccggggta gacgaactcc agacccgacg
     7081 ggtccggcca cgattgcggc tccgtgctgt ccgtgacgta accggtcgct gttttcgtca
     7141 cggttccggg gaggccctcg gtgcgggccg ctcgtacggc gggccggccg tccacggtga
     7201 gccgtcgcgg ggcgagggct ccgatcggtg ccgtccagat gccgtcgcgg cccgtcgtcc
     7261 agccggtgat ttcgcggccg ccgctcagtt ccaccttcgc ctgttccggt gtgccgtagc
     7321 cgtccgcctg gtagatcacg cggaaaccgt tcgagccgga atcgccgagt gcgtcgtcga
     7381 agacaagggg tgcctcaaga tggtgcacac ctgtgccgaa ggtgacgatg acgtcgccgg
     7441 tcattccggc ggtgacggcg cggacggcct cctgtgctgc tttcggggtg ccgaacggcc
     7501 ggtcggcggt gcctgcccag tcgtctctac cctcggggga cacgaagaac ctgatctcgg
     7561 gcatgctgaa gcgctcctgt ggagtggttt atgggatata ctgaggttta tcagctaaac
     7621 acgaacgagc gacgagcgtc aaggggagtg cgggtggcgg aagacgacgc gcagccggat
     7681 ccggagcgtg cgatcgagct gctgtggggg actcccgagc cggagcgccc ggggttgagc
     7741 ctcggccgga tcgtcgcggc ggccgtcgaa gtcgcggacg cggaagggct ggccgccctg
     7801 tcgatgcgca aggtcgcgga acggttcggc accacgacca tgtcgctcta ccggtacgtg
     7861 cccggcaagg cggagctggt cgaactcatg cgcgacgccg tgtacggcag gtttccgatg
     7921 gagccttcgg acgggctcgg ctggcgggcg gggctcgaac ggtgggcgcg gcgagcatgg
     7981 gagatgcatc gccgccaccc gtggctggtc gacagtgccg ggagccggcg gctgccaggg
     8041 ccgaacatca tggccggata cgaccacgcg ctggatgtcg cctcacggag tggtctggcg
     8101 ccggcgcagg tgatcgcggt cgtgaacctg gtcggcggat tcgtggagtc cgtcgcgcgc
     8161 caggcggggg agaccgcgga actccagcgg cgcaccggcg tgtcccatga gcgctggtgg
     8221 agcgaacgtg agtcgctgtt cgagcggatg gacgcctacc cggccttgga gcggctgtgg
     8281 aaggacggag ggatggacgc tccgctcgac ccgttcgaat tcggattgca gcgcactctc
     8341 gacggcgtgc aggcgctggt cgaaagccgg gggcgtgagg taatgcgtga cgaaactgag
     8401 cgcggcgagt gtgtcgtctg tggcaaggaa ctggacggcg gggggcgggg caggcctcgg
     8461 gactactgct ccagggcttg ccagcagcga gcctatcgcc gccgtcaggc tgagacttcc
     8521 tgaggtgatc cgtcccgccg gtgggaaccg ggcgggacgg atcacgaccg gtcaggagag
     8581 caggtggtcg aactcgccgt tcttcgcgcc gagaacgaag gccttgatct cgtgcggttc
     8641 gaaaacaagg acggggctgt tctcgccgag cttcgaatcc cgaacgccca catagccctt
     8701 tacggagtcg ttgacttcga cgcaaccgtt cgcgccggcg ctgtagctcg atttccacca
     8761 gccggaaagg ccgagcaggg tgggacgaga agaatcattg ttcattgagt tcccttgcca
     8821 agttttgaat gaaagaccgt gtccgaccag gatgctccgc ttgtgcctgt agccgttcga
     8881 aagtactgcg aaatcggcgg aggtcttcgg tgtcctcgta atagaggcct tcgcgtcggt
     8941 tctcgacgta cacggttccg ggatcaccgc cgaattcgga cgggtagtcc aaaagaatga
     9001 acccgccgtc cgaggcgccg tgggcgcccg cggtgaagga gagcacttga atgtcgatgt
     9061 tcggccgttc cgacagttcg atcaagtgct ccagttgatc acgcatgacc gccggcccgc
     9121 ctacttggcg gcgtaggacg ctctcgtcca ggatcgacca gatatgcggt gcgtccccag
     9181 ggcggtccaa tatctcttgg cgcgccatcc gcaggtcgac catggtttcg agttcggcct
     9241 tgttgttcca gcgtctgcct tctctcatga tgtcgagcgc gtaatcacga gtctggaaca
     9301 aaccgggcag tacgaccagg tcgacggtgc tgatccgggc ggcaccggtt tccaggccca
     9361 aatagaggtt gaaccagtcg aacaccccgt ggtcgaagtt gatccaccag tcgcggccgc
     9421 gtttcgctcg tttggtcagg tcgcggaaga acggtgcccg ctcgggcact ccgtagaaat
     9481 cgagcaggac ttccagatcg gccggtgcgg gcaggtttcg cgaggtttcg atatgtgcga
     9541 tgaccgtcgt ggccttgccg atgcgctcgg ccgcggcggt ccggtcgtag cccgccgctt
     9601 cccggaggcg cttcatctcg agcgcgatcc agcgcttgat ggcggtgggg gtcagcgtgc
     9661 ccacgaactg accacctcct caaggtctcg tcacgaaagg tgaccccagt atcacccgta
     9721 tagcccagag agagcccgta gaccggagtg aatgtatagc tctacattta agccctctac
     9781 atcgggacag tactacattg tagagccggg cccgccactg ggcgggtcga aaaccgggga
     9841 ggggcggacg cgatgggtga ggccgcggtg gtccggtccc gtcccgcttg gtcgggggag
     9901 agggggtgtc cggcgatgat cgagatcgtg atggtggaac gtgctgacgc gatccgccac
     9961 gtctacagcg gagacaacgg ttgaccgagg aggggttccg gtgcgtccac gtatcaaaga
    10021 cgagcaccgc ccggtccggt tcggcgacgg ttggatccgg atcggcgggg aggtgttcgg
    10081 catcgccgcc gagttgaggg actcggacgg tgccgtgtgg gcgttactgg aactcctcga
    10141 cggaacgcga acggtgagcc aggtcgtcac cgacctggct cgtctggttc ccggcacccg
    10201 tcccgatgag gtcacagccg ccatcgaaaa gctcgccgaa ctcgggtatg tcgagaacgc
    10261 ggacgagccg acgtgcaccg acctgaccga gcggcaactc gaacgccact cgagaagccg
    10321 gtcgctgtac cggtggatcg atctgacgag aagaacgtcg agctgggatt tccaggtcct
    10381 tctggctcgc gcgcgggtcg tgctggtcgg cgtgggcggc gtcgggtgca ccgcggcact
    10441 ggggctggtc atgtcgggcg tgggacgcct gcactgcgtc gaaccggacg tggtcgaact
    10501 gtccaatctc ggccgccaga tcctttacac cgaggaacat ctggggctgc ctaaggtgga
    10561 cgtggcggtg gaaaggttgc gcgcggccaa ctcggacgtc gaggtcaccg gcgagccgac
    10621 gcggatcggg aacgcggctt cgctgcgaag gctggccgcc gactgtgacg tcttgatcat
    10681 gaccgccgat catccgccgg agatccggac gtggacgaac caggcgtgcg cctcgaccgg
    10741 tacgccctgg gtgtacgggg gttaccacgg cccgcaggtc aatctgggcg tctaccgccc
    10801 gggcgcgggg ccgtgttacg aatgcgcgcg gctcgccgag cgtgatcgcg acgccgcgcg
    10861 tccgccgctg gcgacgtggc gtccggccgc gagcctgccg gcgcacgcgg ccaacgcggt
    10921 gacggccggt gtcgcgggca atctggtggc gcacgcggtg atgcgcctga tcacgaacgc
    10981 gccgaagctg gcgctgaact gtcagtacgg cttcagtctc gccacgatgg accacagctt
    11041 cgtcaccagc ctggaacggc cgcacgcgga gtgccggacg tgcctggccg ccgggtgacc
    11101 gggtcagtcg tcttcggtca gtcgtcgtga gacgctccgg atcagccggg cggccgcgtc
    11161 gccggacagc gcttcggcgt gcagcgtgcc gaacatgtgc tcgtacaagg cgatctcctc
    11221 cggatccctg atcaggtgct cgccggcgaa cagttccacc acgacgagtt cggcgtcgta
    11281 gatgcggaat ccgtgcagcg gcgcgatcgg catggccttg ctgaagggaa ggatgcgcag
    11341 gtcgacggtg cgcaggtcgg cgagcgagga gagccggtcc agctggccga gctgggtgtc
    11401 gagatcgccg acccggatcc acagcgcggc ctcgccgatc accagcggca tagccttgcc
    11461 cgggtcgtga agttgcgcct gccgttcgat tcggacggcc accatgcgtt cgatgtcggc
    11521 atcggcggcg ccacccgtac gtgccgggcc cgaggggagt gcgagcagtt ctcgcatgaa
    11581 gccgggcgtc tgcacgggcc cgggaagcag cgccggttgg aacgtggcga cgcggctcgc
    11641 ccgctgctcc agttcgagga cggatcgctg tttggcggcg gctccaccgg gtttgcggaa
    11701 ctcgtcgcgg aacgacaccg attccacgcg ggcccgtttg agcagttcga gaaccgagtc
    11761 ctcctggtcg gcggcgcccg ccgcggcgag ccagcccgcg atgtcgtcac cggtcgggaa
    11821 ctgcacggcc gtttcgatct tggacacccg tgattggggc cagccaaggt ttttggcgaa
    11881 ctggttgccg gacaggccgg tggctgcccg tagctcgcgg agtttcgtgg cgagccggac
    11941 gcggtcggag ccgggatggg gcgacgaggt gggcatgatc ttctttcgcg gggtcgaggc
    12001 gaagtgggct cctgctcgtt gcgcgatcat gctactccca cacggggcta gagcgcgaat
    12061 agtacacgaa tagccgaatc agacgaggat agttgacgaa tagaatcgag gagccgcgct
    12121 ggccggacta ggggaggacc gatgttcgcc aactggatcc agatcgccga actcgcactg
    12181 ctgccactgg gctttgggat cggctacgcg gcggggcgga cctgtcgccg ggaggagcgg
    12241 gtgtccttcc gtgaaggggc cacgatcacc caccccggct tggtcgcgga atatcagcac
    12301 ccggccccgg gagcgggtgc cggctggaga tctgcccgat aggccgaacc caggggaggg
    12361 gagcacctca tacgggtgaa gcctccgggt gggggaggga gacggcaggc cccgaaaccc
    12421 gcgccggggc ctgccgtcga cgcccttggg tcgtcgtctg tcaaggtgta taacgaccta
    12481 ttcagcatgg cgttgaaaaa gatcatttca cctagccgga aaagatctgc aaagactgtc
    12541 gggggccctg gctagggttg gcgcgtgatc accgaggtct tactggagca gcccgaagaa
    12601 gatattcgga cgccggtgct tttcaagggt ttcgtggaac cgggagcgca ggcggcccaa
    12661 gcgctgctcg tgctggggaa ggtcgcgcgc acccggttct ggatgcctcc gtccatggtg
    12721 aaccggctca tcgccgagtc cgatccggtg ttcaccgcgg accggacgac cctgcgtgtc
    12781 gaggcctttt ccccgtgctg cggggtttac gccaggctcg atctgctgga cgtgggtgac
    12841 ctcggcgagg gctgcacgaa cgtcgatctg tccgacgcga cgaggaccgt cctcgcggga
    12901 gtcgtggccc aggatccctt gcacttgacc gttcgtggtg aaggaatccg ccttcgcacg
    12961 ctggacgggc ggaacgacga gtccgtggtg ccgctgccgg accgctgggt cgcgggattc
    13021 gccgaggtcg ccgctgtcac ggagggtgcc gtcgagaggg cgaagctgga caaggcggag
    13081 acgcggaact tcctgaggcg gcttcccact gccgtcggcc ggtcgtgggc ggtcgcgtcc
    13141 ggccgcgggc tccggctgac ctcacggccg ggacgtgact cgattccggc gaccggactg
    13201 catcggctgc gagtgatcga gcccctcgtc cggttcgccc aagggctgac cgtctacacg
    13261 cgaccggaca gtgaggtcgc cacctgggtg cttcagctgg accgagcgcg gctcgtcgtc
    13321 acattgtcgc cggagccgtc ccgtggtttc tcgggcgaag gcggctggct gaactcgctg
    13381 gccgccgggc tcgaagcgcc ggatctcgtc gcgggtcgcg ccggtcacga cgtcgtcgac
    13441 ggcacgtggt tcccgcgtga actcccgagc gggaacgctc cggcgaacgg ccgcctggcg
    13501 aaggcccgcg agctcgttgc cgccggagcc gtccggttgt tgccggatgg cggctacgag
    13561 atcacgcgcg ccggctccgt gcaacgcgtc cggctttcgc cggccgggtg cacgtgtgcg
    13621 tggtgggcca agcatcagga gacccggggc ccgtgttcgc acgtcctggc ggcccggatg
    13681 tcatcggcgg agtcgtgatg ccgccttcgc cgctttcgaa ctgcctttga agcgggccag
    13741 ccattccttc gtttcgtcgc tgatcgtccc cgctccgcgt tccacgaggt ccgcggcgag
    13801 ttccagaagc gcgaagatac cgcgtcgcgc atgctccagc tccggcagca aagtgtccag
    13861 tgtggacaga acggctaccg ggtgttcgtt cgcgatgatc gaaagccggg atgccagccg
    13921 gggtgcgatg aggtacgggc cgagcactcc cagtgccgcg ccgagcgcac gggggccgag
    13981 catccgtcgg tcgaggaggg tcagcacggc gtccgtgccc agttggacgg cggtggagcg
    14041 tttcagcgtg agggtggtgg cgaggagcat cgtgccgggg aagcccggga ccgaggggct
    14101 gtccaggaac gggggcagtg cggccttctc cacgggattg ccgaattcgg tgtcgttgaa
    14161 gaagaggtat ccggcgacgg cggcttcgat gtggtcccga tcgtaagggg aggcgaccgc
    14221 ttcgtggcat tcggtgaagc cgagccattc ggtggccggg ccgtcgaacg gcacgcccag
    14281 ccagggaact ttcggcgagg ccgcctccgg agccggttcg atgtccagtg tggtcaggtt
    14341 gtggcggccg ctggggccgg ctttcgccgt ccaggtgagc ggcggtccgg gttctccgcg
    14401 gagcgcggcg gcggggtcgg cggattccgg gtaccgggcg cgggcggcgg cgagggcgat
    14461 ccagccggtt tttcggccgg ccggtccgcc gagcgcgtag cggatgttgt cggcgtgagg
    14521 tccggtgagt tccgaggcga gggcgagggc ttcgggcctg gtgtccgggg cgagtctcag
    14581 gagggcgtcg acgacgtcgt cttcggccgc gtcggggtgt tcgagcatgc gggagacgaa
    14641 cacgacggga tcgagccagc cgccttggtg ggtggcggcg gaaacgggag cggctgtgcc
    14701 ttcgagcagg gcggtgatcc ggtggtggag cggacgtcgc cagtcgccga ccttgggtgg
    14761 gggcggaacg tcgtggccgg tgaggccgag gacgagggtg gcgatcatct tcggcacggc
    14821 cgggctgtat ctctgggcgt cgattctggc ggcgcgtttc accagtggtt tgagggcgtc
    14881 cgggagttcg tccgcgacgc ggtgggcgcc gtcgagggcg gccaggagga ggtcggcttg
    14941 gtcggggtcg gcgagcaggg acgagagggc ttcggcgacc gcggccgggg tgtcggggat
    15001 gtcgagtggg atggtcaggg tcttgcgctg gtcccatggc ggtcgcgtgg gttccgggta
    15061 ttcggcggtc tcgatggcgg gtgcctgggc gggggtgacg ccgagccggg cgatggcgag
    15121 agcgcggacg tcggaggctt cgtgggtgag tgccgcttcg aagacttcgt cggcgaggtc
    15181 gctgagttcg ggccgacggt cggcgatgcg gccggcgagt ttgagggcgc gttcggcggt
    15241 ggtggcgccg cgcccggccg ctgccgcggg gaggtgctgg agcgcgactt cgtcgtcgag
    15301 cgtgccttgt ttgtccacaa gggacagtgc tttgacggcg aaggagacgg tgcgggggag
    15361 gccgcttccg cacagccggg cgtagcggtg ttgtgccttc gtgcgttcct ccggggtggg
    15421 ggcgagcgct tcgtggagcg cggcgtacca ctgggcgcgg aaggcggcga agtcgcgggc
    15481 gaggatgtcc agggtgaggt cgagcagcct ggtgcggtcg acttcaccgg tcttggtgag
    15541 ttcgagcagg gcggtggacc atttgtcctg gtcgcggacg tacttgtcga aggcggcgaa
    15601 gctggcgtct ccgttgcctt cgatcgtgag tagttcccag agttcggtga gggcttcggg
    15661 ttcggttcgg agccgctctg ccaggagttt gcccccgaag cggccggccc agcggaacca
    15721 gccggcgtgg tactcgggtg tccggacggg tttgaggtcg ccgtcgagga tcatgcggcg
    15781 gacggcttcc caggcggtct ggtggccgat gacgcgtagc gcgatcgtct tgcgttcggc
    15841 gagtggccgg tcctggagga cgcgttcgag gatcgtgggt gaggcgatcc agagtttggg
    15901 gagcaggggg tcgctcgcgg ggagggcggc ccagagcgcg gtggcggctt gctggcccct
    15961 gagcgggttg cggacgccgt aggtgttggg cttgatctcg atgaactcgc tgctgtcctt
    16021 gaacgcggcc aggagttcgg ggacgagggc tttccgctcg gcgcgggtga gtgcgaggac
    16081 ccgggtgagg acttccgagg gttggccggt gaggagcgcg ttcagctgca ctggctcatt
    16141 gtgtcgtgag tggtggggac ggtcggagcc aaccgggagt tcgcgaccgg cgcgggctgc
    16201 tgagtgtcgc gaaagccact ttcgggacat cagacgtccc taaagtggct ttcgcgacat
    16261 cacttcgggc cggattgctc gcaccggcga tgtcacgtga ctggatggac gacacgccaa
    16321 ggcacggtcg gttccaaccg tccccaccac tcacgaggtg tcagggcacg aggatggcgc
    16381 ggccgcggat gcgtccggcg tcgaggtcgt cgagcgcggt ttggaactct tcgagtgggt
    16441 agcgcgcggt gtggagtttc acgcggcctt gtgcggccag gaccatgagt tcgcagaggt
    16501 cggtgtagga gccgacgagg ttgccgatca ggttgatctc ggtggagatg acgtcgatgg
    16561 tggggacgtc gatgttctcg ccgtagccga cgacgtggta gtcgccggcg cggcgcagca
    16621 tcgcgaggcc gtctttggtg gcgccgcctt cgccgacgaa gtcgatgacg gtttcggcgc
    16681 cttcgccgcc ggtgaggtcg aggacctggt cgatctgggt gccgtcggcg atgacgccgt
    16741 ggtcggcacc gatggagacc gccagttcga cggcgtcggg gttgcggtcg acgacgacga
    16801 gttcggcggc ggtgagggcc ttgagcacct ggatcccgat gtggccgagg ccgccggcgc
    16861 cgatgacgac gcagcggtcg ccgggcctga gtcgtcgggc ggctttcgcg gcggcgtggt
    16921 aggcggtgag gcccgcgtcg gcgagggcgg cgacgtcggc gggttcgagg gtgtcgtcga
    16981 tgcggacaac gctgcgggct gaggtcttga ggtattccgc gtagccgccc gcggtgtcga
    17041 tgccggggaa ctgggattgg gcgcagtgga cgtcgtcgcc ggatcggcag gcgcggcaga
    17101 ggccgcaggt gatgaggggg tggacgatga ccttgtcgcc ttcggtcacg ttggtgaccg
    17161 cgctgccgac ggcgtgtacc cagcctgcgt tttcgtggcc gatcgtgtac gggagggtga
    17221 cgccggattt ctcggcccat tggccttcga ggatgtggag gtcggtgcgg cagacgccgg
    17281 cgccgccgat gcggacgatg acgtcgtagg ggccggtggg ttcgggttcg gcgacctcgg
    17341 tcagctggag cttctcgtgg taaccggtga cctggatcgc tttcacgatg cgtggacctc
    17401 ctggtttcgg ggtgtctggt cggcggcgga ttcggggtag cgggtgcgca gcaggccgcg
    17461 gcagaagtgg gcgttgccgt cgatcgagat gcggacggag cgggcgaacc ggagcctgag
    17521 cgggatttct tcgcgggggt agggcgtgcc gtggtcgtcg acgaggaccg gtgcctccgg
    17581 tgcggtgtcg agtccgagcg cggcgcgccg gcgcaggagt gcgtgggtgg tgcggtggtc
    17641 gggcaggtcg gcgagggtga tgtcgtggag ccggtcttcg gtcagttccc ggttttcgcg
    17701 caggagcctg gtgagggcgc gttccatggc cgcggtgtgg gctttgcggc ggaaggtgag
    17761 ccggagttcg tcgaggtcct ggtcggcttc gtcgccgaag gtgcctcggt agccggcgtc
    17821 ggcggcgagg ccgcggttga tgaggtcgga gtcgtggtgg tcgtcgagga cgacgtggac
    17881 gcgggagacg ccgtcgagcg cggtgaggac gtccttggcg tcggaggcca tgaggtaggc
    17941 gaagttcggt gagcagaacg acgtcgggag cctcaggtgg acggtgacct cggttccgtc
    18001 gaccgtgaag gagcggacga agccgaggtc ggtgatgggt tcgtcgagtt cggggtcgta
    18061 gacggcgtgg agtgcggtgt aggcgtcgtg ttcgcgcatg tcaggccacc gagacggctt
    18121 gggtttcgag gcggcggacc tggagttcct cggggaccgg gaggtcgtac atcgcggcgg
    18181 cgttgaggcc gaggatcttg cgcttctggt cgttggtcag tggcgcgtat tcggtcatgt
    18241 cctcggggat ctggaagtcg acgaaccgtt cgaccagcca tttcggggtc cagagtgcgt
    18301 agtcgctgga gaactggatg cggtcttcgt cgagccagta gagcagttcg ccgatgatct
    18361 gggcgaagta gcgcgggcgg gtgtggatga acggcatcgc gacggcgagg ccggcgtgga
    18421 cgttgggttc ctgggtggcg atccagcaga agtcttcgag ccgggggagt ccgcagtgtt
    18481 cgacgacgaa gttgaggtcg gtgtagtcgg tggcgacctt gtccacgtcg gcgacgtcga
    18541 aggcgtcgcg gtcgaggggg cggatggtgg ggcccttgtg gatatggatg ttgcggatgc
    18601 cgaggtcgag gcaggtttcc aggtagcggc ggcaccaggg gtcgtcgagt ttgtagccgc
    18661 gggagtcgtc ccgccattcc gcggtgtaga gcttgacgcc tttgaggccg aagcgttcgg
    18721 cgtcgcggcg gagctggtcg aggcctgctt gttcgaggcg tgggtcgaag cagtggttgt
    18781 aggtgagttt gccggggtgc ttcttggtga gggcgaaggc ctcctcggtc tggccgaagc
    18841 cgttgttgta gaaggcgccg aggtgcgcgg gctggaagat ggcgtggtcg acgtagccgt
    18901 cctggaagag gtccttcatc aggcgctcgc cgccgtagta gaggtactcc tcgtaggacc
    18961 acagctcgga ttcggggctg aggttgcggt ggtagtcgta gaagcagtcg atgaactgct
    19021 tgccgtggat gttggcctgg ttctccggcc gggcgtccca gagcgcgacg tgcgcgtcca
    19081 cgatgaagta gctctcgccg tccttggtgt acatgggttt cctccgcgtt cgtgaccgcc
    19141 gttggcacgt tggaggtgaa cgctaggggt gtcggcgcag gtcagggagg cgcgcggtgt
    19201 ctcagtttga gacatcgacg ggcctgtgtg ccggtggccg cggacgtacg ctgtgcggag
    19261 gtccgaacgc agtgtggcgt cccgggaggt cgatgtggag agcgcgaacg cggtgatcgc
    19321 gccgcggttg cgggcgtcct ggcagcgcag cgcccggtac ggcgcgcccg aggacgaggt
    19381 gcggccggtg ttcaccgggt cgctggacac cggttcgctg ctgtacgagt gcggcaacga
    19441 ggtgttgcgc ggcttgcagg caacgttggc gaacgaaccg gtcagcttga tgatcaccga
    19501 cagtgaaggc ctggtgctga gcaggctgtg cacggacgcg tcgatagtgc ggtcgttgga
    19561 ccgggtccat ctggcgccgg ggttctattt cgcggagagc aacgccggga ccaatgggct
    19621 gggtttggcg ctggccgatc gcgcgccgac gctggtgcgg gccgcggagc attactgcac
    19681 gggtttgcgg gggtacacgt gcgcggcggt gccggtgctg gatccgctga ccggggcgtt
    19741 ggcggggagc gtgaacctga cgacgtggtc ggatcagtcg tcggagctgc tgctggcgct
    19801 ggcgcaggcg gcggccggga acacggcggc gttgatgctg gcacgggctt cggggcggac
    19861 tccgcggccg gcgccgcgtg gtgaggtgtt ccgggttcat tcggatcggt tcgatccggc
    19921 tgacgtgccg agtctgtctc cgggttggtc ggacgcgctc ggtgaggtgg gttcggcgtt
    19981 gagccgtggg cgggtggtcg ctgtcgtcgg tgaggccggc gccgggaaga cggcgctcgc
    20041 gtcggccgcg cggcgggcga cgcgcccgag ggatcgggtg ctcaacgcgc gtccgcccgc
    20101 gccggacgac gtcgacacct ggctgacgtt gtggacgccg gagctggaga aggacgggac
    20161 gtgcgtgatc gtctcggggg tggacacgct cccggcgtgg gcggccgcgg agctggccgg
    20221 gttgttcgcc gatgctcggc gggaggggga gccgccgccg ttcgtgctga cggcggagga
    20281 cgccggtgcg ataccggagg cgctcgcccc gctggtggag gtggtggtcg aggttccggc
    20341 gttgcggttc cggccggacg acatcgtgcc gctggctcat catttcgccc gccgcgaccg
    20401 ggggcgcgag gtcgcgttca ccccggccgc ggtgcgggcg ctgaccgctt gtgactggcc
    20461 ggagaacgcc aagcagctgc gccgggtggt gcgggaggcg gcgtcgcggt cggatgtgat
    20521 cgacagccgt catctgccgg cggaggtgtt ctccggggtg gggcatcggt tgagccggtt
    20581 gcaggcgatg gaacgggacg agatcgtgcg ctgtctcgcc gagccggggg ccaccgtggt
    20641 cggtgcggcg gagaagctcg ggatgggccg ggcgacgctg taccggaaga tgggccagta
    20701 cggcatcaag gcgcggcagt tccggtctta gagtgcggcc agcctggcga atccgtcgga
    20761 gccgtggccg gcgtcgatcg cctgcccgac ctgggctcgc gcagcgctga ggacaccgtt
    20821 gtcgaggcct tgcgctcgga tcgcgtcgag gatgtggtcc atcgtcgcgg cggtggactc
    20881 gagtgtgccg gcgtcgcccg agtagttctc ggtggcgacc tgctcggcga gcaggtcgat
    20941 catgtccggg aagaagccga gcagcgcctt ggcgtgcgtg gcgatgtcgg tggcgccgat
    21001 gttctccgcc ttggcgagcg cgagcatgtg gatcaccccg ctcagggagg tccagagcat
    21061 gtcctgcagg gccgcgtcga aggcggcggc ctggccgg
//
