LOCUS       NZ_CP102512            10399 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptomyces sp. CA-210063 chromosome, complete genome.
ACCESSION   NZ_CP102512
VERSION     NZ_CP102512.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CA-210063
  ORGANISM  Streptomyces sp. CA-210063
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10859970)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Jorgensen,T.S., Klitgaard,A.K.,
            Mourched,A.S., Blin,K., Oves-Costales,D., Genilloud,O., Larsen,T.O.,
            Tanner,D., Weber,T., Gotfredsen,C.H. and Charusanti,P.
  TITLE     Identification of the Biosynthetic Gene Cluster for Pyracrimycin A,
            an Antibiotic Produced by Streptomyces sp
  JOURNAL   ACS Chem Biol 17 (9), 2411-2417 (2022)
   PUBMED   36040247
REFERENCE   2  (bases 1 to 10859970)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Assembly Name                     :: M642_aaa1.6.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 55.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 11:12:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,501
            CDSs (total)                      :: 9,410
            Genes (coding)                    :: 9,051
            CDSs (with protein)               :: 9,051
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 359
            CDSs (without protein)            :: 359
            Pseudo Genes (ambiguous residues) :: 0 of 359
            Pseudo Genes (frameshifted)       :: 141 of 359
            Pseudo Genes (incomplete)         :: 302 of 359
            Pseudo Genes (internal stop)      :: 23 of 359
            Pseudo Genes (multiple problems)  :: 101 of 359
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:19:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8254379
            Orig. end                         :: 8264778
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102512.1.
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(244..258)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     misc_feature    complement(398..407)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            519..1127
                     /locus_tag="JIX56_RS36070"
                     /old_locus_tag="JIX56_36070"
     CDS             519..1127
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="JIX56_RS36070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36070"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_257546869.1"
                     /transl_table=11
                     /translation="MVVPVEPTLEQPSIDVELHRRLVYGDESALREAYAAYGALVRRVA
                     VRVTRSPAAAEDVAQEVFAQLWSRPYGYDARRGSLRTWLSMVAHRRAVDWVRSEARHRK
                     DARADDSALHAIPDTGPGPDEAVVDRERSLLLHTALAELPRPQREVVHLAYFAGRTYRQ
                     AAVELGIPEGTAKTRLRSALRKLAESLADPSDPATVRGA"
     gene            1204..2235
                     /locus_tag="JIX56_RS36075"
                     /old_locus_tag="JIX56_36075"
     CDS             1204..2235
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="JIX56_RS36075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36075"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_257546871.1"
                     /transl_table=11
                     /translation="MTNDHDGVRELLAAWAVGALPPGDDRTVPLHLAGCETCAAEAERL
                     RDTVRLLDGTPGPGAAVPGGSVNGSVDGVLATALRARRPRVPEVAPHAAPYAAAVAGLQ
                     ALVPELDGRWGTPVVHDWDAHATVAHLVAADEHLAVPLGVDARLPASHIPEETSAGDAW
                     ARRTADVIAHEHGRTPEETVATWAAQAAALLATPEARDPELAARAVTVMGLRLPVADHF
                     VVRAFEAWIHTDDLGRALGLPVPPPPDEHLERLVRLAVRILGLALKDAPPVLFEITGPT
                     TDTTWVFGSDAEPVTAELTLDPVDFCLLVGGRYAPEEVPRGITGDEAAARNVLERAASL
                     AWL"
     gene            complement(2331..2807)
                     /locus_tag="JIX56_RS36080"
                     /old_locus_tag="JIX56_36080"
     CDS             complement(2331..2807)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045206.1"
                     /locus_tag="JIX56_RS36080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36080"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_257546872.1"
                     /transl_table=11
                     /translation="MEATEKCATTLPSAARGGPVSHAVSRVARLHRNAAGRLLRGLGLH
                     PGQELVMMRLWEGGPARQSDLIRLLDLDPSTVTKMLQRLEQAGHVRRAADPADRRAVLV
                     EATEESGALLEQVERAWEELEAQTLAGLDVGERRQLLVLLGRVESNLCPESTGR"
     gene            2948..4009
                     /locus_tag="JIX56_RS36085"
                     /old_locus_tag="JIX56_36085"
     CDS             2948..4009
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="JIX56_RS36085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36085"
                     /product="alkene reductase"
                     /protein_id="WP_257546874.1"
                     /transl_table=11
                     /translation="MTTAYDPIDLSGTQLANRIAMAPMTRSRAAEGGRPTGLTAEYYAQ
                     RASAGLIVTEGIQPCHVGQGYPNTPGLHDEDQVAAWREVTDAVHAAGGKIFAQLMHTGR
                     IGHPVLLPEGLHPVGVSAVAAQGQVYTHVGPKDFVTPRELSHDEVLDTVQEFAAAARRA
                     IEAGFDGVELHGANGYLIHQFLAPGSNLRTDDWGGSDENRIRFAVETVKAVAEAIGAHR
                     TGLRVSPGNPFNDISEPDPEPTYTALVQALEPLGLAYLHIGEAPGQRELTQRLRKLFSG
                     TLILNPATDGPTGPDDLALVEDGTADIVAFGQLFLANPDLPARLKAGGPYNEADPATFY
                     GGDAKGYTDYPSL"
     gene            complement(4098..4997)
                     /gene="thpD"
                     /locus_tag="JIX56_RS36090"
                     /old_locus_tag="JIX56_36090"
     CDS             complement(4098..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="JIX56_RS36090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36090"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_257551310.1"
                     /sec_met_domain="PhyH (E-value: 1.6e-40, bitscore: 138.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTTMTDITDLYPSRAATEVSVPRQDPVVWGSPGTPGPVSLADLQ
                     AYERDGFLAVEELIAPDEVAVYRAELERLVADPAIRADERSIVEPKSQEIRSVFEVHRI
                     SELFAQLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHRTFLGCAGATPQDNYKKSLQMQDAGTPSDEA
                     LTAMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="JIX56_RS36095"
                     /old_locus_tag="JIX56_36095"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="JIX56_RS36095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36095"
                     /product="ectoine synthase"
                     /protein_id="WP_257546876.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLARERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCVEGEAELTDQETGRSYTITPGTMYLLDGHERHTLRVKEDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5449..6720)
                     /gene="ectB"
                     /locus_tag="JIX56_RS36100"
                     /old_locus_tag="JIX56_36100"
     CDS             complement(5449..6720)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.5e-74.
                     Score: 241.8. Matches aSDomain:
                     nrpspksdomains_JIX56_RS36100_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="JIX56_RS36100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36100"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_257546878.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-77, bitscore:
                     260.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGAVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALETYWADGSVMEKQTRKRGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGIEFHEKARAGRVAQRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLRVLARAVRETV"
     aSDomain        complement(5623..6636)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JIX56_RS36100_Aminotran_3.1"
                     /evalue="3.50E-74"
                     /label="JIX56_RS36100_Aminotran_3.1"
                     /locus_tag="JIX56_RS36100"
                     /protein_end="366"
                     /protein_start="28"
                     /score="241.8"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGAVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSVMEKQTRKRGEQVEQALISITEENLADVKEYRGRGLVWGIEFH"
     gene            complement(6793..7323)
                     /gene="ectA"
                     /locus_tag="JIX56_RS36105"
                     /old_locus_tag="JIX56_36105"
     CDS             complement(6793..7323)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004616.1"
                     /locus_tag="JIX56_RS36105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36105"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_257546879.1"
                     /transl_table=11
                     /translation="MTAAQAEPLTEIVEGIRIDRPDVADGAALWRIAGDSGALDLNSSY
                     SYLLWCRDFADTSAVARNETGEPVGFVTGYVRPERPDTLLVWQVAVDEAHRGLGLAAAL
                     LDGLTARVARRHPLTAVETTITPGNTASERLFAAYAERHGASVERTVLFEAGDFPDGPH
                     QPEVLHRIGPLSP"
     misc_feature    complement(7348..7360)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7454..7468)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.31"
                     /tool="antismash"
     misc_feature    complement(7479..7494)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7489..7504
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(7670..8770)
                     /locus_tag="JIX56_RS36110"
                     /old_locus_tag="JIX56_36110"
     CDS             complement(7670..8770)
                     /NRPS_PKS="Domain: Aminotran_5 (74-310). E-value: 9.8e-28.
                     Score: 89.0. Matches aSDomain:
                     nrpspksdomains_JIX56_RS36110_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477727.1"
                     /locus_tag="JIX56_RS36110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36110"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_257546881.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.1e-21, bitscore:
                     74.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFASIEDRVGRLLATEQDVVITQGEALLPLE
                     GAIRGAAGPGTTALNVITGPYGQTFGNWLRDCGATVIDLAVPFHTAVTAERIRDAFAEH
                     PAIDFVSLVHAEAATGNTNPVAEIGEVVREQGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWARMAANPRAPRSSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACLERIEGEGLDAVMSRHARAAAATRAGTFALGGGLEPYVHAAADAAPVA
                     TTLRVPAGVDASELVAKALAADPALPLAAGGGALAAEMVRVNHYGPDATPGVVHSSLAG
                     LGAALAEFGVGVDLEGARRAAAQAWA"
     aSDomain        complement(7841..8548)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JIX56_RS36110_Aminotran_5.1"
                     /evalue="9.80E-28"
                     /label="JIX56_RS36110_Aminotran_5.1"
                     /locus_tag="JIX56_RS36110"
                     /protein_end="310"
                     /protein_start="74"
                     /score="89.0"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAVTAERIRDAFAEHPAIDFVSLVHAEAAT
                     GNTNPVAEIGEVVREQGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWARMAANPRAPRSSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACLERI
                     EGEGLDAVMSRHARAAAATRAGTFALGGGLEPYVHAAADAAPVATTLRVPAGVDASELV
                     AKALAADPALPLAA"
     misc_feature    complement(8780..8795)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.37"
                     /tool="antismash"
     gene            8981..9844
                     /locus_tag="JIX56_RS36115"
                     /old_locus_tag="JIX56_36115"
     CDS             8981..9844
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="JIX56_RS36115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_36115"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_257546883.1"
                     /transl_table=11
                     /translation="MNSVLGRRTRILAATTATAGLLLVAGCSSDGDGGSGGTKTAAGGV
                     ELVKGGQLTTCTHLPYPPFQSEIDGKVQGFDVSLIDLVAEDLGVKQEILDTPFENFKTG
                     AFLNSGECDLAAAGMTITDERKKNVDFSDPYFEATQAVLAAKKADIGSFADLKGDEDFK
                     VGTQAQTTGEDYAKSQGLDPVSFESSDAVLNGLRAGQVDAVVIDYPVVQGWLKDPVLAD
                     AFEVAEQVNTGEEYGFTVKKGNTKLLAAVNKALADAKSDGTYKKLYEQWIGPYDESAAT
                     ASPSAS"
     misc_feature    10205..10219
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
ORIGIN
        1 gcccgcgcgg ggtgaccttc ctcgtcgtcc cggaggacct cggcggactc acccccctct
       61 tcgccggctg ggtcgcgggg gaggagccgt gggacagctg ctacggcccg gtcgaggaac
      121 tcgcccgctc cgcccgccgg ttcgacgaga gccccagcct gttctcgtac gccggtgccc
      181 gccgctccct cgcgctgatc gaggaactgg gcgtggcgaa cgtacgggat cacgacctcg
      241 cgctggccga ccgcttccgc gcgggcctcg ccggactggg ccacgagccg gtgccggcgc
      301 ccggctcggc gatcgtgtcg gtgcccggaa tcggccaccg gcaggcggaa ttgagccggg
      361 cgggggtcga ggtctccgac cgtgcgggcc atctgcgggc cgccttccac ctgtacaacg
      421 cgcccacgga tgtcgaccgg atcctggacg tcctgtccgg ctgacttccg agacccactg
      481 gcgcggaccg cgcgaggacg ctaggaaggc ttcacgaggt ggtggtgccg gtggagccga
      541 cgctcgaaca gccgtccatc gatgtggagt tgcatcggcg gctggtgtac ggggacgagt
      601 ccgcgctgcg tgaggcgtac gcggcgtacg gggcactcgt ccggcgggtg gccgtccgtg
      661 tcacccgcag cccggcggcc gccgaggacg tggcgcagga ggtcttcgcc cagctgtgga
      721 gcagaccgta cggctacgac gcgcgccggg gctcgctgcg tacctggctg tccatggtcg
      781 cccaccggcg ggccgtggac tgggtgcgca gcgaggcccg gcaccgcaag gacgcccgcg
      841 cggacgactc ggcgctgcac gccatccccg acacggggcc cggcccggac gaggcggtcg
      901 tcgaccgcga gcgctccctc ctgctgcaca ccgccctcgc cgaactcccg cgcccccaac
      961 gggaagtggt ccaccttgcc tatttcgcgg gccgtaccta ccgccaggcc gccgtcgaac
     1021 tgggcatacc cgagggcacc gcgaagacgc ggctccgctc ggccctgcgc aaactggcgg
     1081 agtccctcgc ggacccatcg gaccccgcga cggtgagggg cgcgtgatgg gggcgtacgg
     1141 gaccgcccgg accgcgcggg tcctgcgtat cgtggacggg gcagggcgaa agggcggcgc
     1201 gcgatgacca acgaccacga cggcgtacgg gagctgctgg ccgcctgggc ggtcggcgcg
     1261 ctcccacccg gcgacgaccg gacggtcccc ctgcacctgg ccggatgcga gacctgcgcg
     1321 gcggaggccg agcggctcag ggacaccgta cgactgctgg acggcacgcc ggggccgggt
     1381 gccgccgtac cgggtggctc cgtgaacggg agcgtggacg gtgtcctcgc cacggccctc
     1441 cgtgcccgcc gcccccgtgt ccccgaggtc gccccgcacg ccgcgcccta cgccgccgcc
     1501 gtcgccggac tccaggcact ggtcccggag ctggacggcc gctggggcac gccggtcgtc
     1561 cacgactggg acgcgcacgc caccgtcgcg cacctcgtcg ccgccgacga gcacctcgcc
     1621 gtacccctcg gtgtcgacgc gcgcctgccc gcctcgcaca tcccggagga gacctccgcc
     1681 ggggacgcct gggcccggcg caccgccgac gtcatcgccc acgagcacgg ccgcacaccc
     1741 gaggagacgg tcgccacctg ggccgcgcag gccgccgcgc tgctggccac gcccgaggcc
     1801 cgcgaccccg aactcgccgc ccgcgccgtc acggtgatgg gcctgcggct gccggtcgcc
     1861 gaccacttcg tggtccgcgc cttcgaggcc tggatccaca cggacgacct cggccgcgcg
     1921 ctcggcctcc ccgtaccgcc accgcccgac gagcatctgg agcgcctggt ccgcctcgcg
     1981 gtccgcatcc tcggcctggc cctgaaggac gcgcctccgg tcctgttcga gatcaccggc
     2041 cccaccaccg acaccacctg ggtcttcggc tcggacgccg agcccgtcac cgccgaactc
     2101 accctggacc cggtcgactt ctgcctcctg gtgggtgggc ggtacgcccc ggaggaggta
     2161 ccgaggggca tcacgggaga cgaggccgcc gcacgcaacg tactggagag ggcggcgtca
     2221 ctggcatggc tgtgaggggg cctggtagct gccggtgagg gtcgcgcccc ttcaggggcg
     2281 cggggaaccg cgcgaccagc ccccacccac ccgcagcgtc cccgccaccc ctaacggccc
     2341 gtcgactccg gacacagatt cgactcgacg cggccaagca ggaccaagag ctgtcgacgc
     2401 tcgccgacat cgagccccgc cagggtctgt gcctccagtt cctcccaagc tcgctccacc
     2461 tgctccaaca gagccccact ctcctcggtc gcctcgacga gcacggcccg ccgatccgca
     2521 ggatcggccg cccgccgcac atgcccggcc tgctcaagcc gctgcagcat cttcgtgacc
     2581 gtggacggat cgaggtccag caaccggatc aggtcggact gccgcgccgg tccgccctcc
     2641 cagaggcgca tcatcaccag ctcctgcccg gggtggagcc cgagcccccg caacagccgc
     2701 ccggcggcgt tgcgatgcag ccgggccacc cgggagaccg cgtggctgac cggcccccca
     2761 cgtgccgcgg acggcagcgt ggtcgcacac ttctcggtgg cctccatcga aaactccctg
     2821 gtcagcgcgg cctctcgacc caagggtacc ggtcgcccca aaaaagttgg ccgaccaagg
     2881 aatgggctac agtgctcccc ggttggattg attggccgac cacataatcc actcatcggg
     2941 agttaccgtg accaccgcgt acgaccccat cgacctctcc ggcacgcagc tcgccaaccg
     3001 catagccatg gcgcccatga cccgcagccg cgccgccgag ggcggccgtc ccaccggcct
     3061 caccgccgag tactacgcac agcgcgcctc ggccggcctc atcgtgaccg agggcatcca
     3121 gccgtgccat gtgggccagg gctacccgaa cacgcccggg ctgcacgacg aggaccaggt
     3181 ggccgcctgg cgcgaggtca ccgacgccgt gcacgcggcc ggcggcaaga tcttcgccca
     3241 gctgatgcac accggccgga tcggccaccc cgtgctgctc cccgagggcc tgcacccggt
     3301 cggcgtctcg gcggtcgccg cgcagggaca ggtgtacacg cacgtgggcc ccaaggactt
     3361 cgtgaccccg cgcgagctga gccacgacga ggtcctcgac accgtccagg agttcgcggc
     3421 ggccgcgcgc cgcgcgatcg aggccggttt cgacggagtg gaactgcacg gcgccaacgg
     3481 ctacttgatc caccagttcc tcgcgcccgg ctccaacctg cgcaccgacg actggggcgg
     3541 ctccgacgag aaccgcatcc gcttcgccgt ggagaccgtg aaggccgtcg cggaggcgat
     3601 cggcgcgcac cgcaccgggc tgcgcgtctc gcccggcaac cccttcaacg acatcagcga
     3661 gcccgacccc gagcccacct acaccgcgct ggtccaggcc ctggagccgc tcggcctcgc
     3721 gtatctgcac atcggcgagg cccccgggca gcgtgagctg acccagcggc tgcgcaagct
     3781 gttctccggc acgctcatcc tcaacccggc gacggacggc cccaccggcc ccgacgacct
     3841 ggccctcgtc gaggacggca ccgccgacat cgtcgccttc ggccagctct tcctcgccaa
     3901 cccggacctc ccggcccggc tgaaggcggg cggcccgtac aacgaggccg accccgcgac
     3961 cttctacggc ggcgacgcga agggctacac ggactacccc tcgctgtagt acggccgtgg
     4021 ccgatcggga cgtgtgaagg gccgggcggt cgctcagggg gcagcgaccg cccggcccgg
     4081 tcagggcgac ggcgaggtca ccgcacgggc gtgaagtccc gtgcgccgat gaactccggc
     4141 cgccgtaccg gcgccgcgaa cggctcgacc gccgcgttct ccacgctgtt gaagacgatg
     4201 aagacgttgc tgcgcgggaa cggcgtgatg ttgtccccgg agccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgaacc ggccttgccg gtgaacagct tgatgccgta ctcggaggcc
     4321 atggccgtca gcgcctcgtc ggacggggtg ccggcgtcct gcatctgcag cgacttcttg
     4381 tagttgtcct gcggggtggc ccccgcacag ccgaggaacg tccggtgcga ccccggcatg
     4441 atcatgaggc cgccgttggt gtcgtagttc tcggtcaggg cgatcgagac ggacaccgtc
     4501 cgcatgttcg gcagcccgtc ctcggcgtgc caggtctcga agtcggagtg ccagtagaag
     4561 ccggacgcgc cgaagccggg cttcacattg atccgcgact ggtggacgta gacgtccgag
     4621 ccgaggatct gccgggcccg cccgacgacc cgctcgtcgc gcacgagttg cgcgaacagc
     4681 tcgctgatcc ggtgtacctc gaagaccgag cggatctcct gcgacttcgg ctcgacgatc
     4741 gagcgctcgt cggcgcggat cgccgggtcg gcgacgagcc gctccagctc cgcccggtag
     4801 acggcgacct cgtccggggc gatgagctcc tcgacggcga ggaagccgtc acgctcgtac
     4861 gcctggagat cggccaggga gacggggccg ggcgtgccgg gggagcccca gacgaccggg
     4921 tcctggcggg ggacggacac ctcggtggcc gcgcggctcg ggtagaggtc ggtgatgtcg
     4981 gtcatggtgg tcacgatggt tcagacctcc tcggtgagca gggggtagac gccgttctcg
     5041 tcgtggtcct cccgtcccgt gacgggcggg ttgaagacgc agaggcagcg aaagtcctcc
     5101 ttgacgcgca gcgtgtgccg ctcatggccg tcgaggaggt acatggttcc gggcgtgatc
     5161 gtgtaactcc gcccggtctc ctggtcggtg agctcggcct cgccctcgac gcacacgacg
     5221 gcctcgacgt ggttcgcgta ccacatcgac gtctccgtac cggcgtacag gacggtctcg
     5281 tggagcgaga acccgacccg ttcccgggcc aggacgatcc gcttgctctc ccaggtgccg
     5341 gacgccgact tcacatgccg gtcggttcct tcgatgtcct tgaacgaacg gacgatcacg
     5401 gtttcgcgat gcctcctaga taggtgtttc cggtggtcga ctgtggtctc agacggtctc
     5461 ccgcacggcc cgggcgagga cgcgcagacc ctcgtccagc tcctccgggg tgatggtgag
     5521 ggccggcagc agcttgacga cctcgctctc cgggcccgac gtctcgatca gcaggccgag
     5581 ttcgaaggcc cgctgggcca cccgccccgc gcgcgccttc tcgtggaact cgatgcccca
     5641 caccaggccg cgcccgcggt actccttcac atcggcgagg ttctcctcgg tgatcgagat
     5701 cagggcctgc tcgacctgct caccgcgctt gcgggtctgc ttctccatga cggagccgtc
     5761 cgcccagtac gtctccaggg cggccgtcgc cgtcacgaac gcggggttgt tgccgcggaa
     5821 ggtgccgttg tgctcgcccg gctcccagat gtccagctcc ggcttgaaca ggcacagcga
     5881 catcggcagg ccgtagccgc tgatggactt ggagacggtg acgatgtcgg gcgtgatgcc
     5941 cgcctcctcg aacgagaaga aggcgcccgt gcggccgcag cccatctgga tgtcgtcgac
     6001 gatgagcagc atgtcctgcc gctcgcacag ctcggcgagc gcccgcagcc actcggggcg
     6061 ggcgacgttg atgccgccct cgccctgcac ggtctccacg atcacggcgg cgggcttgtt
     6121 cagcccggag ccctggtcct ccaggagtcg ctcgaaccac aggaagtcct cgaccgcgcc
     6181 gtcgaagtag ttgtcgaacg gcatgggcgt gccgtgcacc agcgggatgc cggccccggc
     6241 ccgcttgaag gcgttcccgg tgacggcgag cgagccgagc gacatgccgt ggaaggcgtt
     6301 ggtgaacgac acgatggcct cgcgcccctt caccttccgg gccagcttca gcgccgactc
     6361 caccgcgttg gtgccggtcg gccccgggaa catgaccttg tacggcaggt cgcgcggccg
     6421 cagcacgagg ttctggaagg actccaggaa cgcccgcttc gccgtcgtcg acatgtcgag
     6481 gccgtgggtg acgccgtccc gctccagata gtcgatcaga gcgcgtttga gtaccgggtt
     6541 gttgtggccg tagttgagtg aaccggcgcc cgcgaagaag tccaggtacg tgtggccgtc
     6601 ctcgtcgaac atgcggctgc cctgcgcgcg gtcgaagacg gtgggccagc cgcggcagta
     6661 gctgcgcacc tcggactcca gggtctcgaa gacgctcagg tcgggctggg tgatggtcac
     6721 gtcgaatcgc tcctcgatgc gtggggggag gggagggagg gggaggggaa gtgtgtgggg
     6781 gtgagggagg gctcaggggg acagcgggcc gatgcggtgg agcacctcgg gctggtgcgg
     6841 accgtccggg aagtcacccg cctcgaacag gaccgtgcgc tcgacactcg cgccatgacg
     6901 ttcggcgtac gcggcgaaca ggcgctcgga cgcggtgttc cccggcgtga tggtggtctc
     6961 gacggcggtc agggggtgcc gtcgcgcgac ccgtgcggtg agcccgtcca gcagcgcggc
     7021 cgcgagcccg agcccccggt gcgcctcgtc gacggccacc tgccacacga gcagggtgtc
     7081 cggccgctcc gggcgcacgt acccggtcac gaacccgacc ggctccccgg tctcgttccg
     7141 cgccaccgcc gaggtgtccg cgaagtcccg gcaccacagc agatagctgt acgaggagtt
     7201 caggtccagg gctccggagt ccccggcgat acgccacagc gccgcgccgt ccgccacgtc
     7261 cgggcggtcg atccgaatcc cttccacgat ttccgtcagt ggttctgctt gtgcggcagt
     7321 catggggctt gaatttaccg aggggaattt cgaattgcat cgccgcgcgg ggttacgcat
     7381 gggccgccca cgtgttatca cgcgggcgtg agaggcccag cgagacatgg tcgatgtggg
     7441 ggtgtttgac cgtaaattac cgaacaaaag gggcgcgttg tggagtcggt cacacgcgcg
     7501 taaccctcac gagatctgct cgaattagcc ccgtcagtgt tcgcgaaacc tttgcgttta
     7561 ggcgagggga gagcgggcag aagaatacgg ggttctgccc tgcgaattcc atgtgaattc
     7621 ctaccggaaa aacttcccac aattcccccg gcggagccgt tccgttccgc tacgcccagg
     7681 cctgagccgc cgcccgccgg gccccctcca ggtccacccc caccccgaac tccgccagcg
     7741 ccgcccccag ccccgcgagg ctcgagtgca cgacgcccgg ggtcgcgtcg gggccgtagt
     7801 ggttcacgcg gaccatctcc gcggcgaggg cgccgccgcc cgcggccagc ggcagggcgg
     7861 ggtccgcggc gagcgccttg gcgaccagct ccgacgcgtc gaccccggcg ggcacccgca
     7921 gggtcgtggc gaccggcgcc gcgtcggccg ccgcgtggac gtacggctcc aggccgccgc
     7981 ccagggcgaa ggtgcccgcg cgggtcgccg cggcggcgcg cgcgtgacgg gacatcaccg
     8041 cgtccaggcc ctccccctcg atgcgctcca ggcaggcctc caacgcgagc atctccagct
     8101 gcgcgggcgc gtggagaagg gcgcggcggc caccgtcgat ccagcgctcc ttccagtcaa
     8161 ggagcgagag gtaggaactc ctgggcgccc gcgggttggc tgccatccgc gcccacgccc
     8221 gctcgctcac ggagatcgcc gacaccccgg ccggcccgcc catcgccttc tgcgccccga
     8281 tgacgcacag gtcgacaccc cacgcgtcgg gcagcaccgg ctcggcgccc acggaggcga
     8341 ccgcgtccag gtagaagagc gccccctgct cgcggaccac ctcgccgatc tccgccacgg
     8401 ggttcgtgtt gccggtcgcc gcctccgcgt ggaccagtga gacgaagtcg atcgccgggt
     8461 gctcggcgaa ggcgtcccgg atccgctcgg ccgtgaccgc cgtgtggaag gggacggcca
     8521 ggtcgatcac ggtcgcgccg cagtcccgca gccagttccc gaatgtctgc ccgtacggac
     8581 cggtgatcac gttcagcgcg gtcgtaccgg gcccggcggc cccgcgaatc gccccctcca
     8641 gcggcagcag cgcctcgccc tgtgtgatca cgacgtcctg ctccgtggcg agcagccggc
     8701 ccacccggtc ctcgatcgac gcgaagtgcg ccgcactcaa cggggccaga tccagaaacg
     8761 ggtgcgtcac ggggtgctct cttcgctcac ggggtagatg gtgtcgaggg taccgacggc
     8821 cccggctcgc ccctcgacga cgacatctga ggccgaacgg cccagtagcc atcggtttgg
     8881 tttgaggaag tcaaagatct ccttataatc ggatgtctgt gttccctgtc ttctccgtct
     8941 tctctgtgtt ccccgcagtt ccctcatagg aggtctcccc gtgaactcgg tcctcggacg
     9001 ccggacccgc atcctggccg ccaccaccgc gacggccggg ctgctgctcg tggccggctg
     9061 ttcctcggac ggcgacggcg gcagcggcgg caccaagacc gcggccggcg gggtggagct
     9121 ggtgaagggc ggtcagctga cgacctgcac ccacctgccg tacccgccgt tccagtcgga
     9181 gatcgacggc aaggtgcagg gcttcgacgt gtcgctgatc gacctcgtcg ccgaggacct
     9241 gggcgtcaag caggagatcc tggacacgcc gttcgagaac ttcaagacgg gtgccttcct
     9301 gaactccggc gagtgcgacc tcgccgcggc cggtatgacg atcaccgacg agcgcaagaa
     9361 gaacgtcgac ttctccgacc cgtacttcga ggccacccag gccgtcctcg ccgccaagaa
     9421 ggccgacatc ggctccttcg cggacctcaa gggcgacgag gacttcaagg tcggcaccca
     9481 ggcgcagacc accggtgagg actacgccaa gagccagggc ctcgacccgg tctccttcga
     9541 gtcctccgac gccgtcctca acggcctgcg cgccggccag gtcgacgccg tcgtcatcga
     9601 ctacccggtc gtccagggct ggctgaagga cccggtgctc gccgacgcct tcgaggtcgc
     9661 cgagcaggtc aacaccggtg aggagtacgg cttcacggtc aagaagggca acaccaagct
     9721 cctcgccgcc gtcaacaagg cgctcgccga cgccaagtcc gacggcacgt acaagaagct
     9781 gtacgagcag tggatcggcc cgtacgacga gagcgccgcc accgcctccc cgtccgcctc
     9841 atgagcgtga ccgacacgga cacaccgctc cagccgaaga agaaggggct gagccggacc
     9901 cagaagcgca ccctctcccg gggcgcgcag tacgcggtgt tcgtcgcggc ggtcatcgcc
     9961 ttcgcggtgt ccgccgactg ggaccagctc aagaaccagt tcgcccagtg ggacatcgcc
    10021 gagcggatgt tccccgacgt catcacgctg gcgctcaaga acaccgtgct gtacacgctg
    10081 tccggcttcg cgttcggcct cgtcctcggc atggtcatcg cgctgatgcg gctgtcgtcg
    10141 gtgggcccgt accgctggtt cgccggtgtg tacatcgaga tcttccgggg cctgcccgcc
    10201 ctgctgatct tcatcttcat cggtgtcgcc gtgccgctgg ccttccccgg cacggagatc
    10261 cccggcggca cgtacggcaa ggtcgccctc gcgctgggcc tggtggcggc ggcgtacatg
    10321 gcggagacga tccgcgcggg catccaggcc gtccccaagg gccagctgga agcggcccgt
    10381 tccctgggct tctcaccgg
//
