LOCUS       NZ_CP102512            11917 bp    DNA     linear   CON 11-FEB-2024
DEFINITION  Streptomyces sp. CA-210063 chromosome, complete genome.
ACCESSION   NZ_CP102512
VERSION     NZ_CP102512.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. CA-210063
  ORGANISM  Streptomyces sp. CA-210063
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10859970)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Jorgensen,T.S., Klitgaard,A.K.,
            Mourched,A.S., Blin,K., Oves-Costales,D., Genilloud,O., Larsen,T.O.,
            Tanner,D., Weber,T., Gotfredsen,C.H. and Charusanti,P.
  TITLE     Identification of the Biosynthetic Gene Cluster for Pyracrimycin A,
            an Antibiotic Produced by Streptomyces sp
  JOURNAL   ACS Chem Biol 17 (9), 2411-2417 (2022)
   PUBMED   36040247
REFERENCE   2  (bases 1 to 10859970)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Assembly Name                     :: M642_aaa1.6.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 55.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 11:12:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,501
            CDSs (total)                      :: 9,410
            Genes (coding)                    :: 9,051
            CDSs (with protein)               :: 9,051
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 359
            CDSs (without protein)            :: 359
            Pseudo Genes (ambiguous residues) :: 0 of 359
            Pseudo Genes (frameshifted)       :: 141 of 359
            Pseudo Genes (incomplete)         :: 302 of 359
            Pseudo Genes (internal stop)      :: 23 of 359
            Pseudo Genes (multiple problems)  :: 101 of 359
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:19:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1482364
            Orig. end                         :: 1494281
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102512.1.
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11917
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11917
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11917
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6917](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6917)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    187..201
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    complement(663..678)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.44"
                     /tool="antismash"
     gene            complement(1910..2719)
                     /locus_tag="JIX56_RS06460"
                     /old_locus_tag="JIX56_06460"
     CDS             complement(1910..2719)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009193050.1"
                     /locus_tag="JIX56_RS06460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06460"
                     /product="YhjD/YihY/BrkB family envelope integrity protein"
                     /protein_id="WP_257537793.1"
                     /transl_table=11
                     /translation="MKLCRFLHARGARVWRRGKEVELMHRAMGFAALGLVTFAPLLIVV
                     AAAAPIEERGFALWIVDGMGLSGRPADAVQDLFSAPRKVLGTISVLSMVLLAVFGVTFA
                     GSVQTGYEKIWDLSAGRWHTVWRRAVWLVVLTAYLFAEAQSSALLGSGTLRSWARLVLS
                     SVLGVLFFWWGQRFLLGGRVSWRALLPGAIATMVGLGGLRLFSHLVFSPLIVSNAASYG
                     AVGTVLIVTSWLIGVGFVVFGGALVGRYWYLHEPHHIPHPHNRSRKH"
     misc_feature    complement(3203..3218)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.74"
                     /tool="antismash"
     gene            complement(3273..4439)
                     /locus_tag="JIX56_RS06465"
                     /old_locus_tag="JIX56_06465"
     CDS             complement(3273..4439)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="JIX56_RS06465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06465"
                     /product="cytochrome P450"
                     /protein_id="WP_257537794.1"
                     /sec_met_domain="p450 (E-value: 2.5e-17, bitscore: 61.9,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGNILFRKTLDERTISRGSADPAATIVPMRMGTAGVGLLINDH
                     SEATRILSDVETYGSAGELMASVFDEPAESHPDSMSEMVFAVNQTDGEEHRRVRRALRR
                     VIDARVRDVSDQLIQDVQHSIESALTLGRLDAVASIGVPIADSVMSRLLGLDAHLVLAL
                     RTAVYQGYPVRELDATLVDWVRDRRARPADDLVSDLMAELPSLDDRHVAANTRLLALSG
                     VEVLAALITNGILCLAQDPGTQSLVRDDETLLPGLVHEVQRYASPIARGIYRMTKESSV
                     IGQYTVPAGTLLVIGVDVCNRDTSAFPDAHRFDPTRGRDVEYLTFGRGRHSCLGIPVTR
                     LIAAEALRAFLSGTTSFGLTVEPSELRFHDTAVMNGLEELPIWVECAN"
     gene            complement(4432..5004)
                     /locus_tag="JIX56_RS06470"
                     /old_locus_tag="JIX56_06470"
     CDS             complement(4432..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="JIX56_RS06470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06470"
                     /product="DUF5825 family protein"
                     /protein_id="WP_257537795.1"
                     /transl_table=11
                     /translation="MTQQTAERRSNRRRLFASVNLHSMQSREDLVTLTRSGYAGVRLVG
                     HFAMSEMGDRELVSLIALLRDARGVGLRVSWSGDCGALEVGCLRHLDPPRQSDGTFAWS
                     AQQGESLVVRRGPTFLAVEDTRYGERRRIDIDRSEPAAAVLDASGWGHTITPVEAASLH
                     ALELHDLVFRSGDHCVGIAVRQGVWCV"
     gene            complement(5001..6917)
                     /locus_tag="JIX56_RS06475"
                     /old_locus_tag="JIX56_06475"
     CDS             complement(5001..6917)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="JIX56_RS06475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06475"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_257550763.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.7e-152, bitscore:
                     508.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQIGHKRPVLDLNPPVVHEDALSVALVCMPWAVVDMPSLALSTIA
                     PLFEQRGEVEKLDVVYANIRWLDFLVREVGLEREHYELIVDAEVFGVGEWVFSSCFRPG
                     TDPRHTSFYDLVSDSEHVDAYCAMFRAAPAFVDELARDLVTSGVDVLGLTTTFDQNIAS
                     FALAHRVKELSPSIVTMLGGANCDGVQGTAVHRARAEFDYVIRGEAERSIAPLIAHIAG
                     RRSGDENGHDKLLRAVPGLCWRSDIDGASVSNPIGPTPVSMNAVPEPVYDHYFRDLRKS
                     PAAQLIDVKIPLEASRGCWWGAKHHCTFCGLNGSAMAHRAKPAERVQAEASRAVRRHNV
                     LDLVFSDNILAPDHVVRLTTDMALPAEWDVRLFAEVKSNLNFGQLESLARAGFTQLQPG
                     VESLCTPILRIMRKGVTGWQNVRFLRDCATSRIYPSWNILVGFPGEQDRDYAALIDSLP
                     SLHHLTPPAGVFPIKLERFSPYFDDPELGLQVLGPSRNLVSAYEGVVDALDDVVYVFES
                     APAGISPEMYEMLREAVADWRANASISRLVVLPAPDDSLVVVDDRSTWPSREVVLHPGL
                     EAEAYRALAKGKTLPALASTMSSLGIAAPEGQLEELLKNWMELGIIFHEDATYLALATG
                     LRW"
     misc_feature    complement(6907..6920)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.92"
                     /tool="antismash"
     gene            complement(6952..8217)
                     /locus_tag="JIX56_RS06480"
                     /old_locus_tag="JIX56_06480"
     CDS             complement(6952..8217)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057931.1"
                     /locus_tag="JIX56_RS06480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06480"
                     /product="MFS transporter"
                     /protein_id="WP_257537796.1"
                     /transl_table=11
                     /translation="MTTTEALDGSAAVDDAARSIARPFYVYAVLANSLFQRGVFVIFLY
                     QRGFSAGQVALLQTLIYLVSGLAEMPTGIIADRIGRRASIVIGQVLIAGCLLGQVTSSN
                     YWVFLALFMLQGVGMACVSGSDTALLYDLLVRRGATAGYVKIKSRFTMLGTVTSGVAIV
                     LGGQLQQFSWGIVYVGSAACLVLAVVVLMSRVPEIRGADAVDEQDGAEAHSDATAWRAM
                     LRVATPALVTLVVVSGLMHATLTPYIIFTQKTLSDQGAGTAVVSVVIAAGFFVGGLVPL
                     LSDRANRRIGYRIVIPVSLVTLAVALGLSGLGLVWVTIAAFLVLAGIPEITAVLVDNVF
                     NEAVPSRHRASLLSMIAFVESVLIGIGYLVLGTLMDGFGSSVGMATYAAVPLLACLLWL
                     PVLFRGARVTTEIEKPADQKAS"
     misc_feature    complement(8491..8502)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(8499..9737)
                     /locus_tag="JIX56_RS06485"
                     /old_locus_tag="JIX56_06485"
     CDS             complement(8499..9737)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579138.1"
                     /locus_tag="JIX56_RS06485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIX56_06485"
                     /product="cytochrome P450"
                     /protein_id="WP_257537797.1"
                     /sec_met_domain="p450 (E-value: 1.4e-13, bitscore: 49.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSQANVPDLESTAVPFFPIPRASGCPLDPAPGLAELQERAPLV
                     RVRLWDDSTPWLVTRHAEQCALLSDRRVSADWMQPGYPFHSQFLRDHHEEGQFLTAMEG
                     PEHLRLRRMIARPFTPRKVEQLRPTVQQVVDDHIDALLAGPKPADFVADFALPIPSITI
                     CHVLGVPYEDHDFFQHAIRTMTGSDVPDDVMNKSQRELYHYLAKLIGRKLAAPEDDLLS
                     QLATERLATGQLNRHQLVMLVLFLLVSGHETTASMIALGTLALLQHPDQITVLKDSDGP
                     EVAAAVDELLRYLTTVQPGRRRVAVEDIEIAGQVIRAGEGLILPEEIGNRDESVFPDAG
                     ELDLRRDASQQLAFGHGVHKCTGQPLARLELQVVFAALFRRIPTLALAADLVDLPFMDD
                     GLGYGVKKMPVSW"
     misc_feature    complement(10218..10232)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     misc_feature    complement(10662..10671)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.04"
                     /tool="antismash"
     gene            10668..10829
                     /locus_tag="JIX56_RS06490"
                     /old_locus_tag="JIX56_06490"
     CDS             10668..10829
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIX56_RS06490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIX56_06490"
                     /product="hypothetical protein"
                     /protein_id="WP_257537798.1"
                     /transl_table=11
                     /translation="MTQALTERSVTMTDEAKPDMVLFTDEEGLDLDDDVELIALSSATL
                     GAIVARRC"
     gene            complement(10998..11522)
                     /locus_tag="JIX56_RS06495"
                     /old_locus_tag="JIX56_06495"
     CDS             complement(10998..11522)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIX56_RS06495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIX56_06495"
                     /product="hypothetical protein"
                     /protein_id="WP_257537799.1"
                     /transl_table=11
                     /translation="MHVEDVRGRQPDVVAHRDGVIRGHRHEVQVRGRLGPAGHGLAVLA
                     RPEAAYRRGIGPVGNGIREGGDRELSLAAAHEVDAVGEAWEGVGEDTERPGSTENHRDI
                     GPEALDLTPDEKARREGVRETGEAENVGIVGEEASAGLVEPVSITGLEGTDPVLVLQVD
                     DDGGMTVRHQW"
     misc_feature    11649..11662
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
ORIGIN
        1 ggaagaccag acccgacacc ccgcgcacga ccgtctcgcg ataggccacg cgaggccggc
       61 cgaccgcgac ctccaggcca ccccgggtct gccggatctt ctccaccgcc acctccagat
      121 gcagttcgcc cctcccggac agcaccgtct gaccggtctc ggggtcggtc cgtacgacca
      181 gcgaggggtc ctcctcggcc agccgcgcca gagccgtcgc cagccggtcg gtgtcggtgc
      241 tccggcgtgc ctcgacggcc accgacacca ccggttcggc gacggacggc ggttcgagga
      301 gcagcggcgc cgtcggcgcg cacagggtcg ccccggcgcg ggccgacttc agcccgacca
      361 ccgcgacgat gtccccggcc accgcccggt ccagctcggc gtgccggtcg gcctggaccc
      421 gcaggatccg cccgatccgc tcgccgcgcc gcgtccccgc gtccagcacc gtgtctccct
      481 tccggatggt tcccgagtac agccgcagat aggtcagccg tccggtggcc gtcgcgctca
      541 ccttgaacgc cagggccgcc aacggagcct gcgcatcggc ggcccgctcc tgttccgtac
      601 cgtcgtgcgt accgcgtacg gccggtacgt ccagcggcga gggcagatac gccacgaccg
      661 cgtcgagcag cggttcgatg ccccggttgc ggtaggcgga gccgcacagc acgaccacgc
      721 cctcgccggt gcgggtcagg tcgcgcagtg ccgaggccag cgtccgcgcg gagagtgtcg
      781 actccgcgca gaactcctcc agcgccaggg gatgcagttc cgccaccgcc tcctccagcc
      841 gccgccggcg tcgttccgcc tcgtcccgca gggcgtccgg caccggcccc tcctcggccg
      901 tgtcgccgcc gtccgcccag accagcgcgc gcatgcgcag caggtccacg acgccggtga
      961 acccgtcctc cgtaccgatc ggcaactgca ccaccagcgg tgccggatgg agccgctgcc
     1021 ggatcgactc gaccgccgtg tcgaggtcgg cgcccgcgcg gtccagcttg ttgacgaagg
     1081 cgatgcgcgg tacgccgtgg cggtcggcct gccgccacac cgactcgctc tgcggctcga
     1141 cacccgcgac cgcgtcgaac acggcgatcg cgccgtcgag cacccggagc gagcgctcca
     1201 cctcgtcggc gaagtcgaca tggcccggcg tgtcgatcag gttgatccga tggccgtccc
     1261 aggcacagct caccgccgcc gcgaagatcg tgatcccgcg atctcgctcc tgcgggtcga
     1321 agtcggtgac cgtcgtgccg tcgtggacct cgccgcgctt gtgggtggtc ccggtggcgt
     1381 acaggaaccg ctcggtgacg gtggtcttcc cggcgtcgac gtgggcgagg atgcccaggt
     1441 tgcggacggc ggtcaggggg ttgtggatgg tgctgcgcat ggcccgtggc ctttcagctg
     1501 agctgtttca gggcagcgcg attcccgtac gaggacggcg gacggcaagg gcatggcgcg
     1561 gacggagctg tcgctccgtc acggcaagag gcttcgacgt caggcgttcg tcacgggcat
     1621 ccggtaccgg ccgcgcagcg ggcaccggac ggacgaggac acgaggatca cctggtacag
     1681 ggaacgggga acgacgacag cgctgcggtc acacatggct gggctcccct cgatcggtgt
     1741 gcgtcgactc gtcacgacgc gcgcgccacg gtgtcgtgcg gcgtgcgctg tgtggcgatc
     1801 ctagggaagc ggacgcgggc ggagcaccac atttttcagc gggctgatcc acggcgcccg
     1861 acaagccggt gcacacccga ccgcggcagc gcttcagtgc ttcagtgctt cagtgcttcc
     1921 gtgagcggtt gtgggggtgc ggaatgtggt gcggttcgtg gaggtaccag tagcgaccga
     1981 cgagggcgcc gccgaagacg acgaagccga ccccgatgag ccatgacgtc acgatcagga
     2041 cggtgccgac cgcaccgtac gaggccgcgt tgctcacgat gagcggggag aagacgaggt
     2101 gggagaacag gcgcaggccg cccagcccca ccatcgtggc gatggcgccc ggcaggaggg
     2161 cgcgccagga gacccggccg ccgagcagga aacgctgccc ccaccagaag aagagcacgc
     2221 cgagcaccga ggacagcacg agccgggccc atgagcgcag ggttcccgat cccaggaggg
     2281 cgctgctctg cgcttcggcg aagaggtagg ccgtcagaac gacgagccac accgcgcgtc
     2341 gccatacggt gtgccagcgt ccggccgaca ggtcccagat cttctcgtaa ccggtctgaa
     2401 cactcccggc gaatgtcacg ccgaagaccg cgagcagcac catgctcagg acactgatcg
     2461 tgcccaggac cttgcggggc gcggagaaga ggtcctgcac ggcgtcggca gggcgcccgg
     2521 acagccccat accgtccacg atccacaggg cgaagccgcg ttcctcgatg ggtgcggccg
     2581 ccgcgacgac gatcagcagc ggggcgaacg tcaccagtcc cagcgccgcg aagcccatgg
     2641 cccggtgcat cagctccact tccttgccgc gccgccatac gcgcgcgccc ctggcgtgga
     2701 ggaagcggca cagtttcatc gagcggtgcc cgcggaggtg cctcctggtc ttgggcgacc
     2761 cggaagaccg cgcagcaccc gatgaaccgg acggctcgga ccgacccggt ggtccgacgg
     2821 gttccatggg ttccaccacg ggctctacag gtccgacggg cggacaggcc ggccgctccg
     2881 ctcctggccg agtgggccac gacgaatctg ccgcagtggt ggcaccaccc catggcgcat
     2941 acgtgctcac ctcgtcttgt tcccatgcac cggcatgggg actcgtcccc gcaccgggcc
     3001 cgcccgtacg tcacgagtgg ctgcttccag cgtgccccgc cgactccaga tgtgcgaccg
     3061 gctcagcggc cggagtgcgc agacgccggg gtgagggcga cgtacttacg ccccgtggag
     3121 gcgacggggg tcggacaagg tccctcccct cgtagcgtag ggcctggatc gccaccacag
     3181 acgagatcct cgtcaaggcc cgactcgtcc agaccagcgc gaagaaaatc gtcaataaca
     3241 acgcgaagta acgcaatcag gatcacgaga cactagttcg cgcactcaac ccaaatcggg
     3301 agttcctcga gaccgttcat gaccgcagta tcgtggaaac gcagctctga cggctccacg
     3361 gtcaggccga aactcgtggt tcccgagaga aaggcgcgca gggcctccgc cgcaatcaga
     3421 cgtgtgactg gaataccaag gcatgaatgc cgcccgcgac cgaacgtcag atattcgaca
     3481 tcccgaccgc gagtggggtc aaacctgtga gcatcgggaa atgctgacgt gtccctgttg
     3541 caaacgtcca ccccgatgac gagcaaagta cctgcgggaa ctgtgtactg gcctatcacg
     3601 gatgactcct tcgtcatccg atagatcccc cgcgcaatcg ggctcgcgta ccgctgcacc
     3661 tcatggacga ggccaggcag cagcgtctcg tcatcgcgta cgagactctg agtacctgga
     3721 tcttgcgcca ggcagaggat tccattcgta atcagtgccg ccaacacctc gacacccgat
     3781 agcgcgagga gtcgagtgtt cgccgcaacg tgtcgatcgt cgagagaagg cagttccgcc
     3841 atcaggtccg acacgaggtc atcggcgggg cgagcacgcc tgtcgcgcac ccagtcgacg
     3901 agcgtggcgt cgagttcgcg caccggatat ccctggtaaa ccgcggtcct cagggccaag
     3961 acgaggtgtg cgtcaagtcc aagcaatcgc gacatgacgc tgtcggcgat gggcacgccg
     4021 atcgaggcga cggcgtcgag cctccccagg gtgagagcgc tctcgatcga gtgttggaca
     4081 tcctgaatca actggtccga gacatcgcgc acgcgagcat cgatgaccct ccgaagtgcg
     4141 cgccgtacgc gacgatgctc ttccccgtcg gtttggttga ccgcgaagac catctcggac
     4201 atcgagtccg gatgcgactc ggccggctcg tcgaaaacgc tggccatgag ttcgcccgca
     4261 ctgccgtagg tttcgacgtc ggacaagatg cgggtggctt cgctgtgatc gttgatgagc
     4321 agtcccactc ctgccgttcc catgcgcatc ggaacgatgg tggctgctgg atccgccgac
     4381 cctcgcgaaa tagtccgctc atcgagtgtc ttgcggaaga gaatgttgcc gtcagacaca
     4441 ccagactcct tgccttacgg caatacccac acagtggtcg ccggaacgaa agacgaggtc
     4501 gtgcaattca agagcgtgca gactggccgc ctctacgggg gtgatcgtgt gcccccaacc
     4561 gcttgcgtca agaacggctg ccgccggttc cgagcggtcg atatcgatac ggcgacgctc
     4621 cccgtagcgt gtgtcctcaa cggctaggaa cgtcggaccg cgtcgaacga caagggactc
     4681 tccctgctgc gccgaccacg cgaaggtgcc gtccgactgc cgtggcgggt cgaggtggcg
     4741 aaggcacccg acctcgaggg caccacagtc gccgctccac gaaacccgca aaccaacgcc
     4801 gcgcgcgtcg cggaggagcg cgatcaggga gacgagttcg cgatcgccca tctccgacat
     4861 ggcaaaatga ccgaccagtc gcaccccggc gtatcccgac cgggtcaggg tgacgagatc
     4921 ctccctgctc tgcatcgagt ggagattcac tgacgcgaag agccttcgtc ggttcgaccg
     4981 ccgctctgct gtctgttgcg tcaccagcgc agtcctgtcg cgagcgccaa ataggtggca
     5041 tcctcatgaa aaatgattcc gagctccatc caattcttga ggagctcttc gagctggcct
     5101 tccggcgctg caatcccgag cgaggacatc gtggaagcga gtgcgggaag cgttttaccc
     5161 ttggccagtg cacggtaggc ctcggcctcg agacccggat gaagcaccac ctcacgggag
     5221 ggccaggtgg accggtcgtc gacgacgacg agggagtcgt ccggcgccgg cagaacgacc
     5281 agccgagaaa tggatgcgtt ggccctccag tccgcgaccg cttcgcgcaa catctcatac
     5341 atttcaggtg atatgccggc gggcgcggac tcaaacacgt agacgacgtc atcgagggca
     5401 tccacgaccc cctcgtaagc gctcaccagg ttgcgcgagg gaccgagcac ctggagaccg
     5461 agttctggat cgtcgaagta ggggctgaag cgttccagtt tgatcgggaa caccccggcg
     5521 ggcggcgtca gatgatggag ggatgggagc gagtcgatca acgccgcata gtcgcggtcc
     5581 tgctccccgg ggaagcccac aaggatgttc cacgacggat agatcctgct ggtggcacag
     5641 tctcgcagga atcgcacgtt ctgccacccg gtcaccccct tgcgcatgat gcgcaggatg
     5701 ggtgtacaga ggctttcgac acccggttgc agttgagtga aaccggcgcg cgcaagggac
     5761 tcgagttgac cgaaattaag gttcgacttc acctcggcga acaaccggac gtcccattca
     5821 gcgggcagcg ccatatccgt ggtcagtcga accacatggt cgggcgccaa aatgttgtca
     5881 gaaaacacca ggtcaagcac gttgtgccga cggacagccc ggctggcctc cgcttgcacg
     5941 cgctcggcag gctttgcgcg atgggccatg gccgacccgt tcaagccgca gaatgtgcaa
     6001 tggtgcttcg caccccacca gcatccgcgg ctggcctcca gaggaatctt gacatcgatg
     6061 agttgggcgg ccggagactt ccgcagatcg cggaaatagt gatcgtagac gggctccggc
     6121 accgcgttca tcgagacggg tgtcggtccg atggggttgc ttaccgaggc tccatcgatg
     6181 tcggatcgcc agcacaggcc aggcacggca cgcagcagtt tgtcgtgtcc gttctcgtca
     6241 ccgctacgac gtcctgcgat gtgggcgatg agtggcgcga tcgagcgctc cgcctcgccc
     6301 cggatcacgt agtcgaactc cgcgcgagcg cggtggactg ccgtaccttg aacgccgtcg
     6361 cagttcgccc cgccgagcat cgtgacgatg ctcggggaca actccttgac gcggtgcgcg
     6421 agcgcgaacg aggcaatgtt ctgatcgaac gtcgtcgtca accccaggac atcgacgcct
     6481 gacgttacga gatctcgggc caactcgtcg acgaaggcgg gcgccgcgcg gaacatcgca
     6541 caataggcat caacgtgctc actgtcgctc acgaggtcat agaaactcgt gtgacgtggg
     6601 tcggtaccgg ggcggaagca gctggagaag acccattcgc cgaccccgaa cacctcggca
     6661 tcgacgatca gttcgtagtg ctcacgttcg aggcccacct cccggaccag gaagtcgagc
     6721 caccgaatat tggcatagac gacgtcgagc ttctcaactt ctccgcgctg ctcgaacaac
     6781 ggagcgatcg tgctcagcgc cagtgagggc atgtccacca cggcccaggg catgcagacc
     6841 aacgcaaccg aaagggcatc ctcgtgcacg accggcgggt tgagatccag caccggtcgt
     6901 ttgtgcccga tctgcatcaa ttccacttcg gctcctcatc gttggcctgt ttcaggatgc
     6961 cttttggtcg gcgggcttct cgatctcggt ggtcacccgc gccccgcgga agagcaccgg
     7021 gagccacagg agacatgcca gcagggggac cgcggcgtag gtggccatgc ccacgctgga
     7081 tccgaacccg tccatgagcg tgccgaggac gagatagccg atgccgatga gcaccgactc
     7141 cacgaatgcg atcatcgaca gcaggctcgc ccggtggcgc gacggcacgg cctcattgaa
     7201 cacgttgtcc acgagcaccg cggtgatctc gggaatcccg gccaggacca ggaacgcggc
     7261 gatggtgacc cagacaaggc cgaggccgct caggccgagt gccacggcga gcgtcacgag
     7321 tgacaccggg atgacgatcc gatacccgat gcgccggttc gcgcggtccg acagcaaggg
     7381 aacgagtccg ccgacgaaga atcccgccgc tatgaccacg ctcaccactg cggtgcccgc
     7441 tccctgatcg gagagggtct tctgcgtgaa aatgatgtac ggcgtcaaag tcgcgtgcat
     7501 caatccggac accacgacaa gcgtcacgag cgcgggcgtg gcgacccgaa gcatcgcccg
     7561 ccatgctgtg gcgtcgctgt gcgcctcggc tccgtcctgc tcgtccaccg cgtccgcgcc
     7621 gcggatctcg ggcacccgtg acatcagcac caccacggcc aggacgaggc acgccgccga
     7681 accgacgtag acgattcccc aggagaactg ctgcagttgg ccgccgagga cgatggcgac
     7741 tcccgaggtg accgtcccga gcatggtgaa ccgggacttg atcttgacgt atccggccgt
     7801 tgcaccacga cgcacgagca ggtcgtacag cagggcggta tccgaaccgg atacacatgc
     7861 catgcccacg ccctggagca tgaacagtgc caggaacacc cagtagttgg aggacgtcac
     7921 ctgaccgagc aggcatccag ctatcagcac ctggccgatc acgatgctgg cacgcctgcc
     7981 gatccggtca gcgatgattc ccgtcggcat ctccgcgagt ccgctgacca ggtagatcag
     8041 agtctgaagg agggccactt gcccggcgga gaagcctcgc tgatagagga agatgacgaa
     8101 tacgccacgc tggaacagcg agttggcgag cacggcgtag acgtagaacg ggcgcgcgat
     8161 gcttcgtgct gcgtcgtcga ctgcagccga cccgtcaagg gcctccgtgg tggtcatgct
     8221 gcgggatccc cctggtgact ccggtgatca ttggcgttgg gcctccgccg tcgaccggaa
     8281 cttaccgtcc ttacttcgcg caggacatcc ggccgaccct ccgggaaccg ttgccggcgc
     8341 cgctgcgatc ctcggtggag gtgccaagtg ccggaaccgg tagcgtgcat ctcctggtaa
     8401 ccctccacca ccggcagact cggctccggc tccggtcggc cgtgcccgat ctgcaacaag
     8461 tccactccgg ctcctgtcac cggctcagcc tcgttcactt accagctcac gggcatcttc
     8521 ttgacgccgt atccgagccc gtcgtccatg aacgggaggt cgacgaggtc ggcggccagg
     8581 gccagtgtcg gaatgcgccg gaacaaggcg gcgaacacga cctggagctc caggcgggcc
     8641 agcggttggc cggtgcactt gtgcacaccg tgcccgaagg cgagctgctg gctggcatcg
     8701 cggcgtaggt ccagctcccc ggcatcgggg aacacgcttt cgtcgcggtt gccgatctcc
     8761 tccgggagga tcagaccctc gccggcgcgg atcacctgcc cggcgatctc gatgtcctcc
     8821 acggccacgc ggcgacggcc gggctggacg gtggtcaggt accgcagcag ctcgtcgacg
     8881 gccgcggcga cctcgggtcc gtcggagtcc ttcaggacag tgatctggtc gggatgttgc
     8941 agcagggcca gcgtgcccag cgcaatcatg ctggctgtgg tctcgtgccc cgagaccagc
     9001 aggaacagga ccagcatcac cagctgatgc cggttcagct ggccggtggc cagccgttcg
     9061 gtggccagct gggacagcag gtcgtcttcg ggggcggcga gcttgcggcc gatcagcttc
     9121 gccagatagt gatacagctc gcgctgggac ttgttcatga cgtcgtccgg gacgtcgctg
     9181 ccggtcattg tcctgatggc gtgctggaag aagtcgtggt cctcgtaagg gacgccgagc
     9241 acgtggcaga tggtgatgga cggaatcggc agggcgaaat ccgccacgaa atccgccggc
     9301 ttcgggccgg ccagcagagc atcgatgtgg tcgtcgacga cttgctggac tgtgggccgc
     9361 agttgctcga ccttgcgggg ggtgaacggc cgggcgatca tccggcgcag ccggagatgc
     9421 tcggggcctt ccatggcggt caggaactgg ccttcttcat ggtggtcccg cagaaactga
     9481 ctgtggaacg ggtagcccgg ctgcatccag tccgcgctga cccggcggtc cgacaggagc
     9541 gcgcactgct ccgcatgcct cgtcaccaac cacggagtgc tgtcgtccca aagccgaact
     9601 cggaccagcg gcgcccgttc ctgtagctcg gcgagtccag gtgccggatc cagcggacaa
     9661 ccagacgccc tgggaatcgg aaagaacggg actgccgtgg attccagatc aggaacgttg
     9721 gcctgcgaag aggtcacaat cctagctccg tcccgaatgt cacataacgg aagacacatt
     9781 aaaagtgggg gccgatggca cccacagcct caagagacct catgaccgcc ggcaggaaga
     9841 ccctgccgga tgccctgaat gactgggctc gctgaatgga gtgatcgccg aatcggcttc
     9901 cacgacctgc ggattccggt ctttgatctt ccgcgccaca tggcgggaaa cgatccctgt
     9961 gcagcccttg tccgctgacg gtgagcagtc tcaaacggca gcctctccgc ttggcactgt
    10021 tccggaccgg cagcagccta cccagattcg atcaagccct acaaggggcg ggaaaggcct
    10081 ggcaaccgtc ggccagggga catcggggca cgtgcgcgat gcgccggaac cgcacgggcc
    10141 gtcggcagtc ccgccccacg aaatgccctc aaccggaaca gtggagtacc actcccccat
    10201 cggcatgaag agggcactga acaggacatt cacgacctac cgcgctgacg actgggggag
    10261 aagcggaatc gattgaccgt cagcggtcct ctcgctcccc cggatcaagc agacccggtg
    10321 tcatcgcgtc cgccgctcag ggagaggtcc ccaccagcaa ggtcgccacc ccccgtacgc
    10381 gatcagattc ggatcatcga acagcgctgc gaaggctccc cgcggcgtgg ggagatcgca
    10441 tcttgtgaac tgccctcgcg atcttgcgtg ctggaagact gaagaactcc catcgtcgca
    10501 ggccacaggg cacttcctca ttcctggcaa catcacagcc acacaacggc cgatgcgtct
    10561 gggaccaggc gtggcaccgg gaagcgtgcg cactgcccat tgacgagacg ccaaagactg
    10621 tatgagataa ggatgttgga accagtcaag gtaaagaggg gtcaaaaatg acgcaggcac
    10681 tgacggagcg ttcggtgacc atgacggatg aggccaaacc ggacatggtg ctcttcaccg
    10741 acgaggaagg tcttgacctt gacgatgacg tcgagttgat cgccctgtca tcggctactt
    10801 tgggcgccat cgttgcgcgg cgctgctgat cgcatctgat gccgactcag aaacgactgg
    10861 ccctctcgtt tgctggagag ggccaggtgt cggttcgatc gtccacgcgg catgctcgcg
    10921 tgacgctcgg ccggcccctg atcaactccg atgtcaattc tccgttcttc gttcctctcg
    10981 gactgctctg catcgcctca ccactgatgg cgcacggtca tcccgccgtc gtcgtcgacc
    11041 tggagtacga gcaccggatc ggtgccttcg agcccagtga tgctgactgg ctcgaccaga
    11101 cctgcgctcg cttcctcgcc gacgatcccg acattctcgg cctcacctgt ctcgcggaca
    11161 ccctcccggc gtgccttctc atcgggcgtc aggtcaagcg cctccggccc gatgtcacgg
    11221 tggttctcgg tggacccggg acgttcggtg tcttccccga cgccctccca cgcttcgccg
    11281 actgcgtcga cttcgtgtgc cgcggcgagg gagagctcgc gatcaccgcc ctcgcggatg
    11341 ccattgccga cgggaccgat cccgcggcgg tacgcggctt ctggtcgtgc aagaacggcc
    11401 aggccgtgtc cagcgggccc cagccgacca cgaacctgaa cctcgtgccg atgcccgcgt
    11461 atgacgccgt cccgatgggc gactacatcc ggctgtcgac cccgcacatc ttcgacgtgc
    11521 acatcggctc cgggtgcacc tactcgtgca agttctgcat gacgtcgacg ttctgggacc
    11581 gcgacttccg gatcaaggat cccgcggtca tcctcgatga gcttcgcttc ctgcaccgca
    11641 cgtacggggt gacccagttc aacttcctgc acgacaactt cgccaacaag cgcacgtatc
    11701 ttgacgagtt cgtgtcctac ttctccgagc acaacgacat gttcgagtgg ggctgcgcgg
    11761 tccgaccgga caatgtcaac ccgtcccttc tgcggcgcat ggccgacgcg ggatgcttca
    11821 acatcttctg cggcacggac gccgggtcgg agaagatcct caaagccatg ctgaagatgc
    11881 cctcgacgaa gcggacctac gagttcttcc ggtccgc
//
