LOCUS       NZ_CP102509            10969 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7098372
            Orig. end                         :: 7109341
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10969
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="28"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10969
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10969
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5969](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5969)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(274..2031)
                     /locus_tag="JIW86_RS33015"
                     /old_locus_tag="JIW86_33015"
     CDS             complement(274..2031)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /locus_tag="JIW86_RS33015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33015"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_257557526.1"
                     /transl_table=11
                     /translation="MTDQGGPGAERELSDARRDADASRLLARADPAEYLSGFALCLYRL
                     GALLMERPEQHQELAEVLAEAVEVTRRLADGDRGAWLPSLAMTLGQRALILGLLRRRGE
                     ARALAAEATDLYRELAHRDPGRYLPDLASALTNLGNQLAELGQHHEALDRMREGVEVRR
                     GLAARQPGSADLELASSLVELGVKLGEMGHHAEAAGVLRDAIGRYRRHTGSPLAQSLLA
                     EPPLAKDVADHWVALVSLTHELSELGRGDETGPYREEAAAVYRRLARTGPHLLAFVTAV
                     ARRNGITLGEDGRFEHPRSTAGRRPSDAPDPAVLARIRKLNEQGLRLASAGSLDLAVGM
                     LRDAVDLCRRAAEVTPGMSIALARSLHNLGLAEAWSGHRAEALAAVDEAVHLCRRTVEA
                     APHLVRPLMADALDSLGSRLSVLGRHHDALVPAEESIRLHRELAREDPTNRLPELARAL
                     NNLGVRLTDADRHEESLAVAREAVDLYRRLRSAEPEGDHLSGLIHGLSNLGLRLARAGY
                     DDEVLAPTTEAVDLLEDLALIHPGNDVAALAESLAWLGHRLSGNGHRREGRAATRAAED
                     LRRRSAVRR"
     misc_feature    complement(1957..1959)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2179..3120
                     /locus_tag="JIW86_RS33020"
                     /old_locus_tag="JIW86_33020"
     CDS             2179..3120
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890544.1"
                     /locus_tag="JIW86_RS33020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33020"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_257557531.1"
                     /transl_table=11
                     /translation="MPVPIIIDCDPGHDDALAIMLAAGDPAVDLLAITTVAGNQTLAKT
                     TLNARRVCTVAGITGVPIAAGCDRPLVQPLVVADDVHGESGMDGPRFPEPTVDVVPEHA
                     VELIHRILTTHPEPVTLVPTAPLTNIALLLTRYPDAAAHIREIVLMGGSTERGNRTPAA
                     EFNIQADPEAADIVFRSGVPITMCGLNVTHQALATPEVLARFEGLGTELGRTCAGLMSF
                     FAATYRRLWGFESPPLHDPVAVARVIEPGLVHCVPASVVVELRGQYTRGATVVDLHRSL
                     DLPVNAQVAVTLETELFWDRLVDAVAALGSRT"
     misc_feature    2194..2208
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     gene            complement(3133..4782)
                     /locus_tag="JIW86_RS33025"
                     /old_locus_tag="JIW86_33025"
     CDS             complement(3133..4782)
                     /GO_function="GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556920.1"
                     /locus_tag="JIW86_RS33025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33025"
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="WP_257559528.1"
                     /transl_table=11
                     /translation="MACGLGATLLGAAAVLSAPGSAFGANLLTNGGFEAAGTPTGWSCT
                     GGLGSVVASPVHGGTKALAGAAGNSDNAKCTQTVAVQPNTTYTLSAWVRGNYVYLGVTG
                     GSSTWTPSATAYTKLTVSFTTGASQTSAEIYVNGWYGQGTYYADDISLDGPGGGADTVA
                     PTTPGSLTSTGKTSSSVSLAWGASTDNVGVTGYDVYQGATKVATSTTTGATVTGLAANT
                     AYGFTVRARDLAGNTSPASNSVSVTTDNTTTPAGFKQAAPYLYLGWGNPPSATTVMNAT
                     GAKWFTMAFILSSGGCNPAWDGQRALTGGIDQSTISSIRAAGGDIVPSVGGWQGNKLGP
                     NCATPEALAGAYQKVIDAYGLKAIDVDIENTDEFENAVVQDRILGALKIVKQNNPGLKT
                     ILTFGTSTTGPTSWGNRLIEQARALGADIDVFTIMPFDFGNASTDMYAGTVSATEGLKT
                     KLKATFGWDDATAYSHIGISGMNGISDTQETTTLQNWTDIRNWANSHHIARLAFWSVNR
                     DRGCPGGGLQESCSGIAQNDWQFTSITAGFTG"
     misc_feature    4915..4930
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.19"
                     /tool="antismash"
     gene            complement(5001..5969)
                     /locus_tag="JIW86_RS33030"
                     /old_locus_tag="JIW86_33030"
     CDS             complement(5001..5969)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="JIW86_RS33030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33030"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_257557533.1"
                     /sec_met_domain="AfsA (E-value: 2.5e-23, bitscore: 80.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVSADHSASPVTALSSALPREYVHKSAHSEVLLTGWRAVAPDEY
                     VITAQWPRAHSFYSPDASGHHDPLLLAESVRQAIPLLSHVAYDVPFGHRQIWDTFSYSM
                     NAEALAVGSTPADIVLRISCSGVTRRGRRLAGLTMHVTATRDGAFLGTAEARFTNQPPA
                     VYQRLRGSNADLDQVAARIIPLPPPLTPRRVGRDRFHDVVLSATSSTHRTQLRADINHP
                     ILFDHPVDHAPGMLLLEAVRQAAYGCTFPRRGVLTDLEIRFFRYAELNSPCWIETTPTP
                     DGTAAAGRLPVRVSARQNGEDVFTATATITAAPARSLCPAL"
     misc_feature    complement(5913..5915)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6021..6034
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.76"
                     /tool="antismash"
     misc_feature    6022..6035
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.62"
                     /tool="antismash"
     misc_feature    6042..6051
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            6074..6739
                     /locus_tag="JIW86_RS33035"
                     /old_locus_tag="JIW86_33035"
     CDS             6074..6739
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883955.1"
                     /locus_tag="JIW86_RS33035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33035"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_215149621.1"
                     /transl_table=11
                     /translation="MSDRKRQRAPQLRAVQTRAAILRAAAEVFDEFGFSGASISKIMKR
                     ADVTQGGMYFHFGSKEELAYAVMVGQGDGLEFPEGEDGLQHLVDITLYLAEQLQHNPVL
                     RAGVRLAVEQGEFGLRDDVAYQAWVLEFRQQLRFARAKGELQPDIDDHELAWVLVSSFT
                     GAQLFSQASTGRADLPQRIASLWRYLLPAVATDDTRANVRLTLRPAQPSQPAEPEQLV"
     gene            6736..7713
                     /locus_tag="JIW86_RS33040"
                     /old_locus_tag="JIW86_33040"
     CDS             6736..7713
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264897.1"
                     /locus_tag="JIW86_RS33040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33040"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_257557534.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 8.3e-11, bitscore:
                     40.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPHVVVTGATGFVGSAVLRRLAAQAPQAVVRAVSRTPPATAHG
                     VRWVDADLAAAESLRGVCDGADVLLSLASYIGPDAERCAAVNSAGTEALVAEARRAGVG
                     RIVQLSTCAVYGPGPHRGQDVGELTPAPVSPASRSRLAGEEPVLEAGGLVLRAGLVLGT
                     GDRWVIPALVDAFRRVPAGWGGGEGLASFVDVDDLARLIVAFALGGGSGGGSGSGDAAR
                     ASGVLHAHHPEPVRNRDLMTALAGHRVLPQRPAGDWPWPTCLDALAGTPGWVSERQFTL
                     LARDHWYRADEAWRLAGIAPGPGPLARLGRAAQWYGERGGLVPG"
     gene            7819..9186
                     /locus_tag="JIW86_RS33045"
                     /old_locus_tag="JIW86_33045"
     CDS             7819..9186
                     /GO_function="GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382473.1"
                     /locus_tag="JIW86_RS33045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33045"
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="WP_257557535.1"
                     /transl_table=11
                     /translation="MPDLSNIVKAYDVRGVVPDEWDESLAELFGAAFVEVAGASAIVIG
                     HDMRPSSPGLSAAFARGAAARGVDVTLIGLCSTDQLYYASGSMNLPGAMFTASHNPAKY
                     NGIKLCRAGAAPVGQDTGLSQIRELVEKWSDEGAPEIAAGTVAGTVTERDSLAGYAAHL
                     RALVDLTVIRPLKVVVDAGNGMGGHTVPTVFEGLPLDLVPMYFELDGTFPNHEANPLDP
                     KNIVDLQARVLAEGADLGIAFDGDADRCFIVDERGQGVSPSAITALVAARELARNGGTG
                     TVIHNLITSWSVPEVVRENGGTPVRTRVGHSFIKEEMAKTGAIFGGEHSAHYYFKDFWN
                     ADTGMLAALHVLAALGGQDGPLSALVASYDRYAGSGEINSTVADQAGRLAAVKAVYGGR
                     EDVTVDELDGLTATGADWWFNVRASNTEPLLRLNVEARDEATLAKVRDEILALIRA"
     gene            complement(9258..10541)
                     /locus_tag="JIW86_RS33050"
                     /old_locus_tag="JIW86_33050"
     CDS             complement(9258..10541)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133275.1"
                     /locus_tag="JIW86_RS33050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_33050"
                     /product="transposase"
                     /protein_id="WP_257557536.1"
                     /transl_table=11
                     /translation="MTAHTRSRALVAGDSALDEFTERLFGHLPRADQRRWARVYLQGLL
                     TTPGKKSVRRLAASVTESPTASQSLQQFINASPWDWDPARTELLRWVEERHTVHAWTIG
                     QVAFPKRGEHSVGVHRRFDPAAGRIVNCQYGFGLFLSLGGMSAPVDWRLLLPETWSADP
                     AMRRRARISPEVAHQTPESLVLELIDSIAARTSSVRAPVIADFSRLNGAATLIGMLGRR
                     GHDFLVSVPPTLPVSAAGLTHGAEPARSPSRAPVRTQARTPVRMPVRTAVHATTAGDFA
                     RLGNTSHPYAALHTGPGAPPQRLRIHSGLVRVPGGETGGPGAQQTYRLFGERRPGPGLR
                     GGPVWLTNMSRHRMDDLLFLARESARSAAALGTLADEFGLLDFEGRSFPGWHHHMTLMS
                     AAFAYKAVPVALRERQEYAPPLDVRRSA"
     misc_feature    complement(10458..10460)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10597..10610)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.56"
                     /tool="antismash"
     misc_feature    10605..10614
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
ORIGIN
        1 cacgagtggt cccccgcctc cgccgaggcc ggcggcggcg cctccgagga gcgggtcgtc
       61 aaggacgcgg tcggcaacgt actgactgat ggcgacaccg tgacggtcgt caagggtctg
      121 aaggtcaagg gcagcacgtc cggcatcaag gcgggcacca aggtgcgcaa catccgcctc
      181 gtcgacggtg tcgacggtca cgacatcgac tgcaagatcg aggggttcgg ccccatgcag
      241 ctgaagtcca gcgtggtccg gaagggctga tcgtcaccgc cggacggccg acctgcgccg
      301 caggtcctcg gcggcccggg tggcggcgcg tccttcccgg cggtggccgt tcccgctgag
      361 gcggtggccg agccacgcca ggctctccgc gagggcggcg acgtcgttgc cggggtggat
      421 caaggccagg tcctccagca ggtccacggc ttccgtggtg ggcgcgagca cctcgtcgtc
      481 gtacccggcc cgggccagcc ggaggccgag gttggacaga ccgtggatca gcccggacag
      541 gtggtcgccc tccggctcgg ccgaccgtag ccggcggtac aggtccaccg cctcccgcgc
      601 gacggcgagc gattcctcgt gacggtcggc gtcggtgagc cggacgccga ggttgttcag
      661 cgcccgggcc agctccggca gtcggttggt ggggtcctcc cgggcgagtt cccggtggag
      721 gcggatggac tcctcggccg gaacgagcgc gtcgtggtgg cggccgagca ccgaaagccg
      781 gctgccgagg ctgtcgagcg cgtccgccat gagcgggcgc accaggtggg gcgctgcctc
      841 gacggtgcgc cggcacaggt gcaccgcctc gtccacggcg gccagggcct cggcgcggtg
      901 accgctccac gcctcggcca gacccaggtt gtgcaaggag cgcgccagcg cgatcgacat
      961 gcccggcgtg acttcggcgg cccggcggca caggtcgacg gcgtcgcgga gcatgccgac
     1021 ggccaggtcg agactgccgg ccgaggccag gcgcaggccc tgctcgttca gcttccggat
     1081 gcgggccagg acggccgggt cgggggcatc ggagggacgc cggccggccg tggaccgcgg
     1141 gtgctcgaac cggccgtcct cgccgagggt gatgccgttg cgccgtgcca cggcggtcac
     1201 gaaggcgagc aggtgcgggc cggtgcgggc gagtcggcgg tagacggccg cggcctcctc
     1261 gcggtacggg ccggtctcgt ccccgcggcc gagttcggac agttcatggg tgaggctgac
     1321 gagggccacc cagtggtcgg ccacatcttt ggcgagcggg ggctcggcga gcagggactg
     1381 ggcgagcggg gagccggtgt ggcggcggta ccggccgatc gcgtcgcgca ggacgcccgc
     1441 cgcttccgcg tggtggccca tctcgccgag cttgacgccc agttccacca gcgacgaggc
     1501 gagttcgaga tcggctgagc ccggctgccg cgcggccagg ccacggcgca cctcgacgcc
     1561 ttcgcgcatc cggtcgagtg cttcgtggtg ctgtcccagt tcggccagct ggttgccgag
     1621 gttggtcaac gccgaggcca ggtcggggag atacctgccg ggatcccggt gcgcgagctc
     1681 gcggtacagg tcggtggcct ccgcggccag ggcgcgggcc tcgccgcggc ggcgcaggag
     1741 gcccaggatc agggcgcgtt ggccgagggt catggccagg ctcggcagcc aggccccccg
     1801 gtccccgtcg gcgaggcgcc gggtgacctc gacggcctct gccaggactt cggccaactc
     1861 ctggtgctgt tcgggccgtt ccatcagcag agcgccgagc cggtacaggc agagtgcgaa
     1921 cccggagagg tactcggcgg gatcggcacg ggccagtaag cgggacgcgt cggcgtcccg
     1981 ccgcgcgtcg ctcagctccc gctcggcgcc cggtccaccc tgatcggtca tctgcccctg
     2041 cccctcctcg cacggaacgt ccccgcttca tcacgtcctg gcgccggggg acggttccac
     2101 cggaccggac gcggcccggg aagaggcaga cttggtgcgg gggatgcccc tgatgccccg
     2161 gatgcccgag gagtgagtgt gcctgttccg atcatcatcg actgcgatcc cggccacgac
     2221 gatgccctgg cgatcatgct ggcggcgggt gaccccgcgg tcgacctgct cgccatcacc
     2281 acggtcgcag gcaaccagac gctcgccaag accaccttga acgcgcgccg cgtctgcacg
     2341 gtcgcgggca tcaccggcgt gcccatcgcg gccggctgcg accgcccgct ggtgcagccg
     2401 ctggtggtgg cggacgacgt ccacggggaa tcgggcatgg acgggccgcg gttccccgaa
     2461 cccaccgtgg acgtggtccc cgagcacgcc gtggaactca tccaccggat cctgaccacg
     2521 cacccggagc ccgtcaccct cgtgccgacg gcgcccctga ccaacatcgc cctgctgctg
     2581 acccggtacc ccgacgctgc cgcgcacatc cgcgagatcg tcctcatggg cggttccacc
     2641 gagcgcggca accgcactcc ggccgccgag ttcaacatcc aggccgatcc ggaggccgcg
     2701 gacatcgtct tccgcagcgg tgtgccgatc accatgtgcg gcctcaacgt gacccaccag
     2761 gcgctcgcca cccccgaggt gctggcccgc ttcgaggggc tcggcaccga gctcggccgt
     2821 acctgcgccg gactgatgtc gttcttcgcc gccacctacc gccgcctgtg gggcttcgag
     2881 agtccgccgc tgcacgaccc cgtcgcggtc gcccgggtca tcgagcccgg actcgtgcac
     2941 tgcgtaccgg cgagcgtggt cgtggagctg cgcggccagt acacgcgggg agccacggtc
     3001 gtcgacctcc accgttccct ggacctcccg gtcaacgcgc aggtcgcggt gaccctggag
     3061 accgagctgt tctgggaccg gctggtcgac gccgtggcgg cgctgggctc ccgtacgtga
     3121 atcagccgtg aatcagccgg tgaagccggc cgtgatggag gtgaactgcc agtcgttctg
     3181 ggcgatgccg gagcagctct cctgcagtcc gccgccgggg cagccgcggt cgcggttgac
     3241 ggaccagaag gcgagccgcg cgatgtggtg ggagttggcc cagttgcgga tgtcggtcca
     3301 gttctgaagg gtggtggtct cctgggtgtc gctgatgccg ttcatgccgg agatgccgat
     3361 gtgggagtag gcggtggcgt cgtcccagcc gaaggtggcc ttgagtttgg tcttgaggcc
     3421 ttcggtggcg ctgaccgtgc cggcgtacat gtcggtcgag gcgttgccga agtcgaacgg
     3481 catgatggtg aagacatcga tgtcggcgcc cagtgccctg gcctgctcga tcagccggtt
     3541 gccccaggag gtcgggcccg tggtgctggt gccgaaggtg aggatggtct tcaggccggg
     3601 gttgttctgc ttgacgatct tgagggcgcc caggatgcgg tcctggacca cggcgttctc
     3661 gaactcgtcc gtgttctcga tgtcgacgtc gatggccttg aggccgtacg cgtcgatcac
     3721 cttctggtac gcgcccgcca gggcctcggg ggtggcgcag ttggggccga gcttattgcc
     3781 ctgccagccg ccgacggacg ggacgatgtc gccgcccgcg gcccggatcg agctgatggt
     3841 gctctggtcg atcccgccgg tgagggcgcg ctggccgtcc caggccgggt tgcagccgcc
     3901 cgaggagagg atgaacgcca tggtgaacca cttcgcgccg gtcgcgttca tcaccgtggt
     3961 cgcgctcggc ggattgcccc atccgaggta gaggtacgga gcggcctgct tgaagccggc
     4021 cggggtggtc gtgttgtcgg tggtgacgct gacgctgttc gaggcgggtg aggtgttgcc
     4081 cgccaggtcg cgggcgcgga cggtgaagcc gtacgcggtg ttcgcggcga gtccggtgac
     4141 ggtcgcaccg gtggtggtgg acgtggccac cttggtggcg ccctggtaga cgtcgtagcc
     4201 ggtgacgccg acgttgtcgg tggaggcccc ccaggccagc gagacgctgg aggaggtctt
     4261 gccggtggag gtgagactgc ccggcgtggt cggggcaacg gtgtcggcgc cgccgccggg
     4321 gccgtccagg ctgatgtcgt cggcgtagta agtgccttgt ccgtaccagc cgttgacgta
     4381 gatctcggcg ctggtctggg aggcgccggt ggtgaaggag acggtgagct tcgtataggc
     4441 ggtggcggag ggcgtccagg tggaggatcc gcccgtgacg ccgaggtaca cgtaattgcc
     4501 gcggacccac gcggagagcg tgtaagtggt gttgggctgg acggcgacgg tctgggtgca
     4561 cttcgcgttg tcgctgttgc cggccgcgcc ggccagcgcc ttggtgccgc cgtggacggg
     4621 cgaggccacg accgagccca ggccgccggt acaggaccag ccggtcggcg taccggccgc
     4681 ctcgaagccg ccgttcgtga ggaggttggc accgaacgcc gatccgggcg cgctcagcac
     4741 ggcggccgcg ccgagcaggg tggcgcccag accgcaggcc aggcgagtgc gagtacgact
     4801 atgggaacgg gtgcgcgtgc tgctgcgggt gcgcaaggga gtgcctccgg gaggtgaggg
     4861 gtgcggcacg gggagcgtgg ccgggaacct attcctccgg atggaccgcc gtcaataggt
     4921 ctggaccaat tgggcggtgg gccggtcgcc cggtgttccg gcggctgcgc atgactcgtg
     4981 ccggaacacc ggacgccgcg tcagagggcg gggcagaggg agcgggcggg cgccgcggtg
     5041 atcgtcgcgg tggcggtgaa gacgtcctcg ccgttctgcc gggccgagac gcgaacgggg
     5101 aggcggcccg ctgcggccgt cccgtcgggg gtgggggtcg tctcgatcca gcagggcgag
     5161 ttgagctccg cgtagcggaa gaaccgtatc tccaggtcgg tcaggacccc tcgccgcggg
     5221 aaggtgcagc cgtacgcggc ctggcggact gcctccagga gcagcatgcc cggggcgtgg
     5281 tcgacggggt ggtcgaagag gatcgggtgg ttgatgtcgg cccggagctg ggtgcgatgg
     5341 gtggagctcg tcgcggagag gaccacgtcg tggaagcggt cgcggcccac gcggcggggc
     5401 gtcagcggcg gcgggagcgg gatgatgcgg gcggccacct ggtcgaggtc cgcgttgctg
     5461 ccgcgcaggc gctggtagac ggccggcggc tggttggtga agcgggcctc ggcggtgccc
     5521 aggaacgccc cgtcgcgcgt ggccgtgacg tgcatcgtga gcccggccag tcttcggccc
     5581 cggcgcgtga cgcccgagca gctgatccgc agcacgatgt cggccggcgt cgaaccgacc
     5641 gccagggcct ctgcgttcat cgagtaggag aacgtgtccc agatctgccg gtgcccgaag
     5701 gggacgtcgt acgcgacgtg gctcaggagc gggatggcct ggcggaccga ttcggcgagg
     5761 aggaggggat cgtgatggcc gctcgcgtcc ggcgagtaga agctgtgggc gcgcggccac
     5821 tgggcggtga tgacgtactc gtcgggcgcg acggcacgcc agcccgtgag caggacttcg
     5881 gagtgagcgc tcttgtgcac gtactccctc ggtaacgcgc tgctgagtgc ggtgacgggc
     5941 gaggcggagt ggtcagctga cacagtcatg gttgagcccc ccttgaaacg tcggacatga
     6001 gctggtgctg tgcggcggtg ggttctaaaa taaaggatgt tcgttttttt tgtcgaggaa
     6061 gttaggtacc ccgatgtcgg accgtaagcg gcaacgcgcg cctcagctaa gggcagttca
     6121 gaccagagcg gccatcctgc gcgcggcggc ggaggtcttc gacgagttcg gtttcagcgg
     6181 agccagtatc agcaagatca tgaagcgggc ggacgtcacg cagggcggga tgtacttcca
     6241 cttcggctcg aaggaggagc tcgcctacgc cgtgatggtc gggcagggcg acggcctgga
     6301 gtttcccgag ggggaggacg ggctacagca cctggtggac atcacgctct acctggccga
     6361 gcagctccag cacaatccgg tgctccgggc cggcgtacga ctggcggtgg agcaggggga
     6421 gttcggcctg cgcgatgacg tcgcgtacca ggcctgggtc ctcgagttcc gccagcagct
     6481 gcgcttcgcg cgggccaagg gggagctcca gccggacatc gacgaccacg agctggcgtg
     6541 ggtcctggtc agctccttca ccggggcgca gctcttctcc caggcgtcca ccggacgggc
     6601 cgacctgccg cagcggatcg cctcgctgtg gcgctacctg ctgcccgccg tcgccacgga
     6661 cgacacccgc gcgaacgtgc gcctgacctt gcggccggcg cagccgtcgc agccggcgga
     6721 accggagcaa ctggtgtgag cgcgcctcac gtcgtcgtca ccggggcgac gggcttcgtg
     6781 gggtcggcgg tgctgcgccg gctggccgcg caggcgccgc aggcggtggt gcgcgcggtg
     6841 tcccgtacgc ctccggccac ggcgcacggg gtgcgctggg tcgacgcgga tctggccgcc
     6901 gcggagtccc tgcgcggggt gtgcgacggc gcggacgtcc tgctgtcgct ggcctcctac
     6961 atcggaccgg acgccgaacg gtgcgccgcg gtcaactccg ccgggaccga agccctggtg
     7021 gccgaggcga ggcgggcggg cgtggggcgc atcgtccagc tgtcgacttg cgcggtctac
     7081 gggccggggc cgcatcgcgg acaggacgtc ggcgagctga cacccgcccc ggtatcgccg
     7141 gccagccgca gccggctggc gggcgaggaa ccggtgctcg aggcgggggg actcgtcctg
     7201 cgcgcggggc tggtgctggg gacgggcgac cggtgggtca taccggctct cgtcgacgcc
     7261 ttccggcggg taccggccgg gtggggcgga ggcgaggggc tggcctcgtt cgtcgacgtc
     7321 gacgacctgg cgcggctcat cgtggcattc gcgctcggcg gcggcagtgg cggcggcagt
     7381 ggcagtggcg acgcggccag ggcctcgggc gtcctgcacg cccaccatcc ggagccggtg
     7441 cgcaaccggg acctcatgac cgccctcgcc ggccaccggg tgctgccgca gcgccccgcc
     7501 ggcgactggc cgtggcccac gtgcctggac gccctggccg ggaccccggg ttgggtgagc
     7561 gagcgccagt tcacgctgct ggcccgggac cactggtacc gcgccgacga ggcatggcgg
     7621 ctcgcgggga tcgcaccggg gcccggaccg ctggcgcggc tcggccgggc ggcgcagtgg
     7681 tacggggaga ggggcgggct cgtcccgggg tgacggcgat ctgccaaagc ctcagcggga
     7741 tgggggcggg cgggccctgg cggggagggc ggcgggtagc gtccggaaca ccgacggtca
     7801 ccccctgaag gagtggaaat gcccgacctt tcgaacatcg tcaaggcgta tgacgtgcgt
     7861 ggtgtcgtgc cggacgagtg ggacgagtcg ttggcggagc tgttcggtgc cgcgttcgtc
     7921 gaggtggcgg gtgcttcggc gatcgtgatc ggtcatgaca tgcggccgtc gtcgccgggt
     7981 ctgtcggctg ctttcgcgcg tggtgcggct gcccggggtg tggacgtgac gctgatcggt
     8041 ctgtgctcga cggaccagct gtactacgcg tcggggtcga tgaatctgcc gggtgcgatg
     8101 ttcacggcct cgcacaatcc ggccaagtac aacggcatca agctgtgccg ggcgggtgcg
     8161 gctccggtgg gtcaggacac gggtctgtcg cagatccgtg agctggtgga gaagtggtcc
     8221 gatgagggcg ccccggagat cgcggcgggc acggtcgcgg gcacggtcac cgagcgggac
     8281 tcgctggccg gttacgcggc gcatctgcgc gctctggtgg acctgacggt gatccgtccg
     8341 ctgaaggtgg tcgtggacgc gggcaacggc atgggcggcc acaccgtccc caccgtgttc
     8401 gagggcctgc cgctggacct ggtcccgatg tacttcgagc tggacggcac gttccccaac
     8461 cacgaggcga acccgctgga tccgaagaac atcgtggacc tgcaggcccg tgtgctggcg
     8521 gagggcgcgg acctgggcat cgcgttcgac ggggacgcgg accgctgctt catcgtggac
     8581 gagcgcggcc agggcgtctc cccgtcggcg atcacggcgc tggtggcggc gcgtgagctg
     8641 gcccgcaacg gcggcacggg cacggtgatc cacaacctga tcacctcctg gtcggtgccg
     8701 gaggtggtcc gggagaacgg cggcaccccg gtccgtaccc gggtggggca ttccttcatc
     8761 aaggaggaga tggcgaagac gggtgcgatc ttcggtggtg agcactcggc gcactactac
     8821 ttcaaggatt tctggaacgc ggacaccggg atgctggccg cgctgcacgt gctggcggcc
     8881 ctgggcggcc aggacggtcc gctgtcggct ctggtggctt cctatgaccg ttacgcgggc
     8941 tcgggggaga tcaattcgac ggtcgcggac caggcgggcc ggctggcggc ggtcaaggcc
     9001 gtctacggcg gccgtgagga cgtcacggtg gacgagctgg acggcctgac cgccaccggt
     9061 gcggactggt ggttcaacgt ccgtgcctcc aacaccgaac cgctgctgcg gttgaacgtg
     9121 gaggcccgcg acgaggccac cctggccaag gtccgcgacg aaatcctcgc gctgatccgc
     9181 gcctgacgat ccgcgcctga cttcgcacgc ggcggggctg cgagcagccc cgccgcaccc
     9241 ggacacgtcc ggctcgttca ggcggaccgg cgtacatcga gcggcggtgc gtactcctgc
     9301 cgctcccgca gggccaccgg aacggccttg tacgcgaacg cggcggacat cagcgtcatg
     9361 tggtggtgcc agccagggaa ggaccggcct tcgaagtcga ggaggccgaa ctcgtccgcc
     9421 agcgtgccga gggccgcggc ggaccgggcc gactcccggg cgaggaacag caggtcgtcc
     9481 atccggtggc ggctcatgtt ggtgagccag accggaccgc cccgcagacc gggtcccggg
     9541 cgcctttcgc cgaacagacg gtaggtctgc tgcgcccccg ggccccccgt ttccccgccc
     9601 ggcacccgga ccagacccga gtggatgcgc agccgctgcg gtggcgcgcc ggggccggtg
     9661 tgcagcgcgg cgtacggatg gctggtgttc cccagacgag cgaagtcgcc cgcggtggtc
     9721 gcgtgcacag ccgtccgtac gggcatccgt accggcgtcc gggcctgcgt ccgcaccgga
     9781 gcccgggaag gactccgggc tggctcggcc ccgtgcgtca gcccggcggc gctgaccgga
     9841 agggtcggcg ggacggacac caggaagtcg tggcctcgtc ggccgagcat cccgatcagc
     9901 gtggcggcgc cgttcaggcg gctgaagtcg gcgatcacgg gggcgcgcac cgacgaggtg
     9961 cgggctgcga tcgagtcgat gagctcgagg acgagggatt ccggggtctg gtgcgcgacc
    10021 tccggtgaga tgcgtgcccg gcggcgcatc gcgggatccg ccgaccaggt ctcgggaagc
    10081 agcagccgcc agtcgacggg cgcgctcatg ccgcccaggg acaggaagag gccgaagccg
    10141 tactggcagt tgacgatgcg tccggcggcg gggtcgaagc ggcggtgcac gccgaccgag
    10201 tgctcgccgc gcttggggaa ggccacctgc ccgatcgtcc aggcgtgcac cgtgtgtcgt
    10261 tcctccaccc agcggagcag ttcggtgcgc gccggatccc agtcccaggg gctcgcgttg
    10321 atgaactgct gcaaggactg ggaggcggtg ggggactccg tcacggacgc ggcgagcctc
    10381 cgtacggact tcttgcccgg ggtggtcagc agtccctgca ggtagacccg ggcccagcgg
    10441 cgctggtcgg ctctcggtaa gtggccgaag aggcgttcgg tgaactcgtc cagggcgctg
    10501 tccccggcaa ccagagctct gctcctcgtg tgtgccgtca tctgtctccc ccttcgctgc
    10561 ggacgccggc gtgcgtcgta ggtaccatca gaatacagaa cgttcgtttt ttttctagcg
    10621 taggagtggc gccctccccg cgacttgggc acgttggtag ccggggtacg tcgtgaggac
    10681 ggcggtggcg ggggtgcggg tgcttcttcg tcaagtcggc gttgtgcgtg gtcggcaccc
    10741 ggggcgacgc tggaggtcgc catcccgacc taggacgtat tggcgagtca ggaacgacct
    10801 ggatggcagt gattgtgcag tggtaaagca agagcgcggc gtgcgcaccc gaaatcatct
    10861 gatctcggtg gccgccgctg aattcgaccg caacggatat gacgggacgt cattatcccg
    10921 gctgagccgc tccgccggca tatccatagg agccctcacc tttcacttt
//
