LOCUS       NZ_CP102509            11281 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2471094
            Orig. end                         :: 2482375
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11281
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11281
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11281
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6281](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6281
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1027..2280
                     /locus_tag="JIW86_RS11730"
                     /old_locus_tag="JIW86_11730"
     CDS             1027..2280
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550369.1"
                     /locus_tag="JIW86_RS11730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11730"
                     /product="MFS transporter"
                     /protein_id="WP_257553705.1"
                     /transl_table=11
                     /translation="MYLAANGRRPDAPPRTPGARRRVPAAVLALGAVSLVTDVSSEMVT
                     AVLPLYLVLGLGLSPLQFGFLDGMFNGATALVRLLGGRLADRGGRHKRVAGTGYLLSAL
                     SRLGLLLAGGATAGIAAALAADRLGKGIRTAPRDALISLSGPPEALGRSFGVHRAMDTT
                     GALLGPLAAFAVLWATADAYDAVFAVSFCVGLLGVLLLVAYVPAGPAAAAGPATPLKAT
                     ALRDPAFRRILYAAALLGAATIGDAFLYLLLLRGLDLPPALFTLLPLGAAAVYLLLAVP
                     AGRIADRFGRRPAFLLGHGALLCAYAVLLAPVSWPTVVAVLALLGIFYAGTDGVLMALA
                     APVLPAHGRAGGLAVLQTGQALARLLGAAGFGAAWTFWGQRPALWAAALALAGALAAAW
                     RILPARATSPATLSPEPS"
     gene            2277..3248
                     /locus_tag="JIW86_RS11735"
                     /old_locus_tag="JIW86_11735"
     CDS             2277..3248
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641835.1"
                     /locus_tag="JIW86_RS11735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11735"
                     /product="hypothetical protein"
                     /protein_id="WP_257553706.1"
                     /transl_table=11
                     /translation="MNLRHRRLRLALVVAAVLLLAGGSLGYVLHARNRDRPARQGEANA
                     SFSLAEPGLYFLDPATGRVARQPGPDVPRVTGGPSCDRFHAAGEHALCLRKLPGVPART
                     EARVLDRGLREIRRVTVPGIPNRARVSASGQLLSWTTFTTGDSYSTSAFSTRTSILDLR
                     TGYLIKSMEQIPLTVDGARHHDSDVNYWGVSFARDDNRFYATVSTRGLTHLVEGDLRNW
                     SAKALRTNAECPSLSPDDSRIAFKKKVSDDPRAPWRLYVLDLATMGEHPLAEPHSVDDQ
                     PAWLDGATVGYGLGGDIRSVPADGTGAPRLLVPGGSSPAYVR"
     gene            complement(3311..3982)
                     /locus_tag="JIW86_RS11740"
                     /old_locus_tag="JIW86_11740"
     CDS             complement(3311..3982)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267203.1"
                     /locus_tag="JIW86_RS11740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11740"
                     /product="AIM24 family protein"
                     /protein_id="WP_215142958.1"
                     /transl_table=11
                     /translation="MKSDLFASENLAQAAVAPGMTLQNAKSVKYAVNGEMLARQGSMVA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMSVRGQGEAWFAHQAGNCFIIDFEPGDALT
                     INGRNVLCFDPTLSYEIKMLKGAGMTGGGLFNSLFTGTGKLAVVCEGHPIIIPVTAQAP
                     VYVDTDAVVGWSAQLETGLHRSQSVGSMIRGGSGEAVQLMLRGEGFVIVRPSEVTETAA
                     H"
     gene            4054..4806
                     /locus_tag="JIW86_RS11745"
                     /old_locus_tag="JIW86_11745"
     CDS             4054..4806
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267202.1"
                     /locus_tag="JIW86_RS11745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11745"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_257553707.1"
                     /transl_table=11
                     /translation="MSSQHPNDTTDVPAVGADSPFRQEHVLRDEAPQFVLPLVVRIEKT
                     EPPARTDALETAARAVLVMLTDERSRGEGEWAEAVRDWQDARIRKVVRRARGAEWRKAQ
                     TLPGVTVHGDTSEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPVAPVTGPGLPVLWFN
                     PALDMSAGKEMAQAGHAAQLAWWELTDAERAAWQDSGFALAVRTAPRERWAELSGSGLP
                     VVRDAGFTEIAPGSATVIADHPALRARL"
     gene            5001..6281
                     /locus_tag="JIW86_RS11750"
                     /old_locus_tag="JIW86_11750"
     CDS             5001..6281
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267201.1"
                     /locus_tag="JIW86_RS11750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11750"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_257559292.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-88, bitscore: 295.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHLGVGIGWRPEIADAVERLPGLDWVEVVAENICPGHLPEALLR
                     LRERGVRMVPHGVSLGLGGSDRPDPGKLAALGERAVALGAPLVTEHIAFVRTSSPALEA
                     GHLLPVPRTRDALAVLCENVRIAQDALPVPLALENIAALFSWTGEELTEGQFLTELVER
                     TGVRLLIDVANLHTNRVNRGEDPAAVLDAIPLEALAYVHVAGGVERGGVWHDTHAHPVP
                     PVVLELLAGLRSRVDPPGVLLERDDDFPPEPELAAELAAIRAVVTAGPAVPADPRPACE
                     ADTRPAPPDEATRTRVGLGQAALLSALVAGTPVPEGFDRQRIRVQSRALAAKRAGVVAR
                     LAPELPAFLGGEEPYRTAFLAYAKGRPMTAGYRRDALDFAEHLLIQDLPADPAARRRLT
                     AWWRDRAGARPPRRVGRWARALVGRAA"
     gene            6278..7306
                     /locus_tag="JIW86_RS11755"
                     /old_locus_tag="JIW86_11755"
     CDS             6278..7306
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956906.1"
                     /locus_tag="JIW86_RS11755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11755"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_257553708.1"
                     /transl_table=11
                     /translation="MNLLALAIWIAVATSSGLLLLGLYRARPRSAGPAPVLHDLSEAAF
                     MAGGPGAVVDTALVSMLGDGRLMVGGPGIVHVRPGARAGDPAERAVLQAHRGAPSGWLY
                     QVRYAAMLDPAVQETGDALAARGLISPPGWGRTWRRWGLIQATVCGLLVPVSLPLTFVA
                     FVMDPDGQVPFIVKVLPVLLGGILVGALGAAAAGRRITPAGRRALREIRSHYVNDRTPH
                     VQTALFGLRGLRDPFLRGQLVPAARGTRLAAAQSGSRPAHRSDSSSWSSAEILPVLWCA
                     GSDGGGSGGGSGSGCGSGSSCGSSGGSSGGGCGSGSSSCSSGSSSSSSCSSSSGSSCSS
                     SS"
     gene            7467..9107
                     /locus_tag="JIW86_RS11760"
                     /old_locus_tag="JIW86_11760"
     CDS             7467..9107
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (108-326). E-value: 3e-20.
                     Score: 64.8. Matches aSDomain:
                     nrpspksdomains_JIW86_RS11760_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130503.1"
                     /locus_tag="JIW86_RS11760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11760"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_257553709.1"
                     /sec_met_domain="PF00561 (E-value: 1.9e-24, bitscore: 85.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTTPARHAAAAAALTLALCTAAAVTAAPAAAAAAPDAESAGADL
                     AARRAASTGLAFRACPAVEALPDPVRCATLRVPLDYARPGGPQIALTVSRVPASRAGGA
                     ARQGALVHNPGGPGASGMYFPLLAGLPAWQRIAAAYDLVGYAPRGVGRSAPLSCQDPAA
                     RTAGPTQVPVRPSPAYKEQRRAAARAYARGCAERAGAALPYYTTLNNVRDLHVLRAALG
                     EEKLTFAGASYGTYLGAVYATLHPGHVRRMVLDSAVNPDPARVWYRNNLDQAPGFERRW
                     LDFRAWVARHHAAYGLGSTPAAVQASYERVRDAVARTPAGGNIGTGELHAAYLETAYRD
                     EVWPERAAALAAFLRGDPDPLTGQAAPDPAAAGEAENATAVYTAVLCNDASWPADWATW
                     DRDHTELARTAPFETWSNAFLNLPCASWPVRARQRPVAVGTRSARLPRLPRTLIVAAER
                     DGATPYPGALELQRRLGTEAALVTEKGAGTHGVFGGRNDCVDRHVERYLLTGDTSGWRV
                     TCAPHPEPAPVSLDDRAARAQGAVLPPVV"
     aSDomain        7791..8444
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JIW86_RS11760_Abhydrolase_1.1"
                     /evalue="3.00E-20"
                     /label="JIW86_RS11760_Abhydrolase_1.1"
                     /locus_tag="JIW86_RS11760"
                     /protein_end="326"
                     /protein_start="108"
                     /score="64.8"
                     /tool="antismash"
                     /translation="ALVHNPGGPGASGMYFPLLAGLPAWQRIAAAYDLVGYAPRGVGRS
                     APLSCQDPAARTAGPTQVPVRPSPAYKEQRRAAARAYARGCAERAGAALPYYTTLNNVR
                     DLHVLRAALGEEKLTFAGASYGTYLGAVYATLHPGHVRRMVLDSAVNPDPARVWYRNNL
                     DQAPGFERRWLDFRAWVARHHAAYGLGSTPAAVQASYERVRDAVARTPAGGNIGT"
     gene            complement(9220..9933)
                     /gene="hemQ"
                     /locus_tag="JIW86_RS11765"
                     /old_locus_tag="JIW86_11765"
     CDS             complement(9220..9933)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072526.1"
                     /locus_tag="JIW86_RS11765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_11765"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_215142966.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLKDVLPEDRAGFADE
                     VQELFDQLAAKDITVRGTYDVSGLRADADVMIWWHAETADELQTAYNLFRRTKLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADETARDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWLLAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
ORIGIN
        1 ggcggcctac gcgaaggcgt accgcgtcga gatgtccgcc gacggcgcct cctggacgtc
       61 cgtcgccacc gaggccgcgg gcaacggcgg cgtcgacgac tggaccggcc tgtccggcaa
      121 ggggcggtac ctgcgcgtcc acggaaccgc gcgcggcacc gcgtacggct actcgctctt
      181 cgaggccgag gtgtacggca ccctcgacgg cggaccgccg cccggcggcg ccttcacggt
      241 ggtcgcggcc ggtgacatcg ccgcccagtg cacggcctcc gacagcggct gcgcgcaccc
      301 caagaccgcc gcgctcgccc agcggatcga cccgaagttc tatctgacga tgggcgacaa
      361 ccagtacgac gacgcccgga tctccgactt ccgcacctat tacgacaaga gctggggcgc
      421 cttcaaggcc aagacccggc cggtgccggg caaccacgag acctacgacc cggccggctc
      481 cctcgccggg tacaaggcct acttcggaag catcgccttc ccgcagggca agagctggta
      541 cagctacgac gagggcaact ggcacttcat cgccctcgac tccaactcct tcgaccaggc
      601 cgcccagacc gactggctca aggccgacct cgccgccaac accaggcagt gcgtcgccgc
      661 ctactggcac cacccgctgt tctcgtccgg cgggcacggc aacgacccgg tctccaagcc
      721 cgtctggaag atcctctacg ccgccaaggc cgacctcgtc ctgaacggcc acgaccacca
      781 ctacgagcgg ttcgccccgc aggacgccga cggcagggcg accgccgacg ggatcgtgga
      841 gatcgtcggc gggatgggcg gcgccgagcc gtacccgatc gaacaggtcc agcccaacag
      901 ccagaagcgg atcagcggtc cctacggcgt ggtgaagctg gagttctccg actccggcta
      961 cggatggacg tacgtcgcca cggacggcca ggtcaaggac acgagcccga agtacagctg
     1021 ccactgatgt acctggccgc caacggccgc cgcccggacg cgccgccccg caccccaggg
     1081 gcccggcggc gcgtccccgc cgccgtcctc gcgctcggcg cggtcagcct ggtcaccgac
     1141 gtctcctccg agatggtcac ggcggtgctg ccgctctacc tcgtcctcgg actcggcctc
     1201 tcccccctcc aattcggctt cctggacggc atgttcaacg gcgcgaccgc actcgtgcgg
     1261 ctgctcggcg gacgtctcgc cgaccgcggc ggccgccaca agcgcgtcgc ggggacgggc
     1321 tacctgctct ccgcgctgtc ccggctcggg ctgcttctcg ccgggggcgc caccgccggg
     1381 atcgccgccg cgctcgccgc cgaccggctg ggcaagggca tccgcaccgc accgcgcgac
     1441 gccctgatct cgctcagcgg ccccccggag gcgctcggcc ggtccttcgg ggtgcaccgc
     1501 gccatggaca ccacgggcgc cctcctcggg ccgctcgccg cgttcgccgt gctgtgggcc
     1561 accgccgacg cctacgacgc ggtgttcgcc gtcagcttct gcgtcggcct gctcggcgtg
     1621 ctcctgctcg tcgcgtacgt cccggccggg cccgcggcgg cggccgggcc cgcgactccc
     1681 ctcaaggcca cggcccttcg cgaccccgcc ttccgccgca tcctgtacgc cgccgccctg
     1741 ctcggggccg ccaccatcgg cgacgccttc ctctacctgc tgctcctgcg cggtctcgac
     1801 ctgccgcccg ccctgttcac gctgctgccg ctcggcgcgg ccgccgtcta cctgctgctc
     1861 gccgtcccgg cgggccggat cgccgaccgg ttcgggcgac ggcctgcctt cctgctcggg
     1921 cacggcgccc tgctctgcgc gtacgccgtg ctgctcgcgc ccgtctcctg gccgaccgtc
     1981 gtcgccgtac tggccctcct ggggatcttc tacgcgggca ccgacggcgt cctgatggca
     2041 ctcgccgctc ccgtgctgcc cgcgcacggc cgggcgggcg gactcgcggt cctgcagacc
     2101 ggccaggccc tggcccggct cctcggcgcc gccgggttcg gggcggcgtg gaccttctgg
     2161 gggcagcggc ccgccctgtg ggcggcggcc ctcgcgctgg ccggggcact cgcggccgcc
     2221 tggcgcatcc tgcccgcccg cgccacctcc cccgccaccc tctccccgga gccctcatga
     2281 acctccgtca ccggcggctc cgtctcgcct tggtcgtcgc cgccgtactc ctcctggcgg
     2341 gcggctccct gggctacgta ctccatgcga ggaaccgcga ccggccggcc cgacagggcg
     2401 aggccaacgc ctcgttcagc ctcgcggagc ccggcctcta cttcctggac ccggcgaccg
     2461 gccgggtcgc tcgccaaccc ggccccgacg tgccgcgcgt gaccggcggc ccttcctgcg
     2521 accgcttcca cgccgccggg gaacacgccc tgtgcctgcg caagctgccc ggcgtcccgg
     2581 cccgcaccga ggcccgcgtg ctcgaccggg gcctgcggga aatccgccgg gtgaccgtcc
     2641 ccggcatccc caaccgggcc cgcgtctccg cctcggggca gctcctgtcc tggacgacgt
     2701 tcacgaccgg cgactcctac tccacctccg ccttctcgac ccggacctcg atcctggacc
     2761 tgcgcaccgg ctacctgatc aagtcgatgg agcagatccc gctgaccgtc gacggggccc
     2821 ggcaccacga ttccgacgtc aactactggg gagtgagctt cgcccgcgac gacaaccgct
     2881 tctacgccac cgtctccacc cggggcctca cccacctcgt cgagggcgac ctgcggaact
     2941 ggtcggcgaa ggcgctgcgc acgaacgccg aatgcccctc gctgtcaccg gacgacagcc
     3001 gcatcgcctt caagaagaag gtctccgacg acccccgggc accctggcgg ctgtacgtcc
     3061 tggacctggc gacgatgggg gaacacccgc tggccgagcc gcacagcgtc gacgaccagc
     3121 ccgcctggct ggacggcgcg accgtgggct acggcctcgg cggcgacatc cggtcggtcc
     3181 cggcggacgg tacgggagcg ccccggctcc tcgtcccggg cggctcgtca cccgcgtacg
     3241 tgcggtgacc gggccccggc agtacgcgtg ggccggcgcg ggccgtggcg ccggcccacg
     3301 ccggccgggt tcagtgggcc gccgtctcgg tgacctcgct cgggcggacg atgacgaagc
     3361 cctcgccgcg cagcatcagc tggacggcct cgccggagcc gccgcggatc atcgagccga
     3421 ccgactggga acggtgcagg ccggtctcca gctgggcgct ccagccgacc accgcgtccg
     3481 tgtccacgta gaccggggcc tgggcggtga ccgggatgat gatcgggtgg ccctcgcaga
     3541 cgacggcgag cttgccggtg ccggtgaaga ggctgttgaa gaggccgccg ccggtcatcc
     3601 cggcgccctt cagcatcttg atctcgtagg acagggtcgg gtcgaagcac agcacgttgc
     3661 gcccgttgat ggtcagcgcg tcgccgggct cgaagtcgat gatgaagcag ttgcccgcct
     3721 ggtgggcgaa ccaggcctcg ccctggcccc gtacggacat cagcgccagg ccctcgcccg
     3781 tgacggcgcg cttgagcatg ccgcctatgc cctgcccctt gcgctcgaac tgcaggttgc
     3841 cgcggaaggc gaccatcgag ccctgacggg ccagcatctc gccgttgacg gcgtacttca
     3901 cggacttggc gttctgcagg gtcatgccgg gggcgacggc cgcctgggcc aggttctcgg
     3961 acgcgaaaag atcactcttc atggcgtcga tcctcgcccg gcccggaccc ccgcggcatc
     4021 atcctggagg cggagcctgg catccttggc gggatgagca gccagcaccc gaacgacacc
     4081 accgacgtcc ccgcggtcgg cgcggacagc cccttccggc aggagcacgt tctgcgcgac
     4141 gaggccccgc agttcgtcct gccgctggtg gtgcggatcg agaagaccga gccgcccgcc
     4201 cggacggacg ccctggagac ggcggcccgc gccgtgctgg tcatgctgac cgacgagcgg
     4261 tcgcggggcg agggcgagtg ggccgaggcg gtgcgggact ggcaggacgc gcggatccgc
     4321 aaggtggtcc ggcgggcgcg cggggcggag tggcgaaagg cgcagaccct gccgggtgtc
     4381 acggtgcacg gtgacacctc ggaggtacgg gtgttcccgc cggtgcccct ggacggctgg
     4441 cccaaggagc tggccaagct ccaggtgtcg ggcaccgacc tggacgaccc ggagccggtc
     4501 gccccggtca ccgggccggg tctgcccgtg ctgtggttca acccggcgct cgacatgtcg
     4561 gccggcaagg agatggccca ggccgggcac gcggcgcagc tggcgtggtg ggagctgacg
     4621 gacgcggagc gcgccgcgtg gcaggactcc ggtttcgcgc tggccgtacg gacggcgccg
     4681 cgtgagcggt gggcggagct gagcggcagc gggctgccgg tggtccgcga cgccggtttc
     4741 acggagatcg cgcccggcag tgcgacggtg atcgccgacc acccggcact gcgcgcgcgg
     4801 ctctgacgcc ctcccggtcc gggggacggt ccgggggacg gtccgggcaa aggtccgagg
     4861 aaggtccggg aggcgtcccg gggaacctca aatgttgctc tgaacgtctc tcttcgcgat
     4921 tggcgcgacc ccgccggggc catcacatcg ctacacggtg cgattcactg cgctgagagg
     4981 gggcggggga catgaagccc atggcacacc tgggggtggg catcggctgg cggccggaga
     5041 tcgcggacgc ggtcgagcgg ctgcccggcc tggactgggt cgaggtggtg gccgagaaca
     5101 tctgcccggg ccatctgccc gaggcgctgc tgaggctgcg cgagcgcggc gtccgcatgg
     5161 tgccgcacgg ggtctcgctg ggtctcggcg gctcagaccg ccccgacccg gggaagctgg
     5221 cggcgctcgg ggagcgggcg gtggcgctgg gggcgcccct cgtcaccgag cacatcgcct
     5281 tcgtccgtac ctcctcgccc gcgctcgagg ccggccacct gctgccggtg ccgcgcaccc
     5341 gcgacgcgct ggccgtgctg tgcgagaacg tgcgcatcgc acaggacgcg ctgcccgtgc
     5401 cgctggcgct ggagaacatc gccgcgctgt tctcgtggac gggcgaggag ctgacggagg
     5461 ggcagttcct gacggagctg gtcgagcgga ccggcgtacg gctgctcatc gacgtggcca
     5521 acctgcacac caaccgggtc aaccggggtg aggacccggc cgccgtgctg gacgcgatcc
     5581 cgctggaggc gctggcgtac gtgcacgtgg cgggcggtgt cgaacggggc ggtgtctggc
     5641 acgacaccca cgcgcacccg gtgccgccgg tggtgctcga gctgctggcc gggctccgct
     5701 cccgggtgga cccgccgggc gtcctgctgg agcgggacga cgacttcccg ccggagccgg
     5761 agctggccgc cgagctggcc gcgatccgcg ccgtggtgac ggccgggcct gccgtaccgg
     5821 cggacccccg gcccgcgtgc gaggcggaca cccggccggc gccgcccgac gaagccaccc
     5881 gcacccgggt ggggctcggg caggccgcac tgctgtcggc cctggtggcc ggtacgccgg
     5941 tccccgaggg gttcgaccgg cagcggatcc gggtgcagtc ccgggcgctg gccgccaagc
     6001 gggccggggt cgtggcccgg ctggctcccg aactgcccgc attcctgggc ggcgaggagc
     6061 cgtaccgcac ggccttcctc gcctacgcca agggccgccc gatgaccgcc ggctaccgcc
     6121 gggacgcgct ggacttcgcc gagcacctgc tgatccagga cctgccggcc gacccggccg
     6181 cccggcgccg gctgaccgcg tggtggcggg accgggccgg ggcgcggccg ccccgccggg
     6241 tcgggcgctg ggcccgggcg ctcgtgggga gggcggcatg aacctgctcg ccctggcgat
     6301 ctggatcgcc gtcgccacgt ccagcggcct gctgctcctc ggcctgtacc gggcccggcc
     6361 ccggtcggcc gggccggcgc cggtcctcca cgacctgtcc gaggccgcgt tcatggcggg
     6421 cgggcccggc gccgtcgtgg acaccgccct cgtctcgatg ctgggggacg gccggctgat
     6481 ggtcggtggc ccgggcatcg tccacgtacg ccccggggcg cgggcgggcg accccgccga
     6541 gcgcgccgtc ctgcaggccc accgcggtgc gccctcggga tggctctacc aggtgcggta
     6601 cgccgccatg ctcgacccgg ccgtccagga gaccggggac gctctggcgg cccgcgggct
     6661 gatctcgccg cccggctggg ggcggacctg gcggcgctgg ggcctgatcc aggccaccgt
     6721 gtgcggactg ctggtgccgg tgtcgctgcc gctgaccttc gtcgcgttcg tcatggaccc
     6781 cgacgggcag gtgccgttca tcgtcaaggt gcttcccgta ctgctaggcg gaatcctagt
     6841 gggggcgctg ggcgccgcgg cggccgggcg gcggatcacc ccggcggggc ggcgggcgct
     6901 gcgcgagatc cgctcccact acgtgaacga ccggaccccg cacgtgcaga ccgcgctgtt
     6961 cgggctgcgc ggcctgcggg atccgttcct gcgcgggcag ctggtgcccg ccgcgcgcgg
     7021 cacccggctg gccgcggccc agtcggggtc ccgtcccgcg caccgctccg actcctcgtc
     7081 ctggtcctcc gccgagatcc tgccggtgct gtggtgcgcc gggagcgacg gcggcgggag
     7141 cgggggcgga tcggggtccg gctgcggctc ggggtcctcc tgcgggagct ccggtgggag
     7201 ctcgggtggc ggctgcggaa gcggcagttc gagctgttcc agcgggagtt ccagcagttc
     7261 cagctgtagc agttcgtccg gttccagctg ttccagcagt tcctgacggc ggtcgccgtt
     7321 cccgggcggc gggcggcgcc ggggtgcggc aaccgggggt ggatcccacg gcaacggggc
     7381 cattgagggc gacaccgccc ggtgttcctt tacttacgtg cggcgccctg gtcgtctcat
     7441 cggaccatcc gcaagaaggg gccgccatgc ggacgacgcc cgcacgccac gcagccgccg
     7501 cggcggccct cactctcgcc ctgtgcacgg ccgccgctgt caccgccgcc ccggcggcag
     7561 cggccgccgc cccggatgcc gaatcggccg gcgccgacct cgccgcccgc cgtgccgcct
     7621 ccaccggact cgccttccgg gcctgtccgg ctgtggaggc actgcccgac cccgtgcgct
     7681 gcgcgaccct gcgcgtccca ctcgactacg cccgccccgg cggcccgcag atcgccctca
     7741 ccgtcagccg ggtaccggcg agccgcgccg gcggcgccgc ccgccagggc gcgctcgtcc
     7801 acaaccccgg cggccccggc gcctccggca tgtacttccc gctgctcgcc ggactccccg
     7861 cgtggcagcg gatcgccgcc gcctacgacc tcgtgggcta cgcgccgcgc ggcgtcggcc
     7921 gctccgcccc gctgtcctgc caggacccgg ccgcccgcac cgcgggcccc acccaggtcc
     7981 cggtccgccc ctcccccgcg tacaaggagc agcgccgcgc cgccgcccgg gcgtacgccc
     8041 gcggctgcgc cgagcgggcc ggcgcggccc tgccgtacta cacgacgctg aacaacgtcc
     8101 gcgatctgca cgtgctgcgc gccgcgctcg gcgaggagaa gctgaccttc gcgggcgcct
     8161 cgtacggcac ctacctgggc gcggtctacg ccaccctcca cccgggccac gtccgccgca
     8221 tggtcctcga ctcggcggtc aaccccgatc cggcccgcgt ctggtaccgc aacaacctcg
     8281 accaggcacc cgggttcgag cggcgctggc tcgacttccg cgcctgggtc gcccgccacc
     8341 acgccgcgta cggcctcggc agcaccccgg cggccgtgca ggcgagctac gaacgggtcc
     8401 gggacgcggt ggcccgtacc ccggcgggcg gcaacatcgg caccggtgag ctgcacgcgg
     8461 cgtacctcga gaccgcctac cgcgacgagg tctggccgga gcgggcggcg gccctggccg
     8521 ccttcctgcg cggtgatccc gacccgctga ccggccaggc ggccccggat ccggccgcgg
     8581 ccggcgaggc cgagaacgcg acggccgtgt acacggcggt gctgtgcaac gacgcctcct
     8641 ggcccgcgga ctgggcgacc tgggaccgcg accacaccga gctggcccgc acggcgccct
     8701 tcgagacctg gtccaacgcc ttcctgaacc tgccgtgcgc ctcgtggccg gtgcgggccc
     8761 ggcagcggcc ggtggcggtc ggcacccggt cggcccggct gccccggctc ccccgcacgc
     8821 tgatcgtcgc ggcggaacgg gacggggcga ctccgtaccc gggcgcgctg gagctccagc
     8881 ggcggctcgg caccgaggcg gcgctggtga cggagaaggg ggccggcacc cacggagtct
     8941 tcggcggacg caatgactgc gtggaccgtc acgtggagcg gtatctgctg acaggtgaca
     9001 cctcggggtg gcgtgtcacg tgtgcgccgc atccggagcc cgcaccggtg tcgctggacg
     9061 accgggcagc acgtgcccag ggggcagtgc tgccgccagt cgtctgaact tccgggcggg
     9121 atctccggct gcgtcgtttc gggggcaggg cctcccgatc cgccggaagc cactcttgtg
     9181 tcacgagtga tcctccggcc cttcccccca ggggaggtat caggcgagcc cggccaccag
     9241 atcggcgacg gacttgcggc gtccggtgta gaagggcacc tcttcacgga cgtgcatacg
     9301 ggcctcggag gcacgcaggt gacgcatgag gtcgacgatg cggtagagct cgtcggcctc
     9361 gaaggccagc agccactcgt agtcgcccag ggagaacgag gcgacggtgt tggcgcgcac
     9421 gtccgggtaa ccgcgggcca tcttgccgtg gtcggcgagc atacggcgac ggtcctcgtc
     9481 gggcagcagg taccagtcgt aactgcgcac gaaggggtac acgctgacgt agtcgcgggc
     9541 ggtctcgtcg gccaggaagg ccgggatgtg cgacttgttg aactcggccg ggcggtgcag
     9601 ggccatgttc gaccagaccg gctccaaggc gcggcccagc ttggtgcggc ggaacaggtt
     9661 gtacgcggtc tgcagctcgt ccgcggtctc cgcgtgccac cagatcatga cgtccgcgtc
     9721 ggcgcgcagg ccggagacgt cataggtgcc gcggacggtg atgtccttgg ctgccagctg
     9781 gtcgaacagc tcctggacct cgtcggcgaa gccggcgcgg tcctccggga ggacgtcctt
     9841 cagcttgaag acggaccaca gggtgtagcg gatgacctcg ttgaggtcct tcgccttctt
     9901 ccccgcgttg ggaatcttct ctggtgcagt catgtgccta ttgtcccgtg tgctgatcag
     9961 tggtctgcgc caggggtgcc gaacccgcac cgaaggcggc gatcaccgcg tccgccgcct
    10021 tgcccgcgct ggcgatgcac gccggaatgc ccacgccgtc gtagagcgcc ccgcagaccg
    10081 cgaggccggg cagggccgcg acggccgtgc ggatcccggc cacccggccg aggtggccga
    10141 ccgggtactg gggcagcccg ccctcccatc gggtgacggt ggaggccacc ggccgggccg
    10201 cgaggcccac ggccgcgccg aggtcggtga gcgagacgtc gaccagttcg gcgtcctcgc
    10261 gccccaggtc cttctcgtcg ccgtgccggc cgacggaggt gcgcagcagg aagagctccg
    10321 gatcggcccc ggcccaggcc cacttgttgc tggagaaggt ggaggccttg atggtccgcc
    10381 cgtcgacggg cggtacgagg aacccgctcg cgccgccctc ggcgatggcg gcgggcaggt
    10441 cggagcggcg gaaggccatg gtgaccaggg ccatggaggc gtactcgacc ccgcgcagct
    10501 cggccgcggc ggccggcgcg agcccgtcga gcagccgggc ggcggggccg gcgggggtcg
    10561 ccaggatcac gccgtcggcc tcgatgacct ccgcgtcggc gacgacccgc cagccctcag
    10621 gggtacggac gacctcgcgg acgggggttc cggtggcgat cctggccccg gccgcgcggc
    10681 aggcctcggc cacggcggcc gggagccgtc cgatcccgcc gtcgatgccg gcgaagaccg
    10741 gaccggccgg ctggggctgc gcctccgcgc ggtgctgcag ttcccgtacc ccgtcgccca
    10801 gcagtgtgtg cgtacgggcg gcctcgaaga gctggggcac ggcggcccgc atcgagatgc
    10861 ggtaggcgtt gcccgcgtac accccgccga gcagcggttc cacgagccgg tcgacgacct
    10921 cgcggcccag ccgggcggcg acgtactcgc cgacggcgac gtcctcgccg agctcggcgg
    10981 gcggcagcgt gcgctcggcc tcgatccggg cgaggccctc cggggagagc acccccgagg
    11041 cggcgagcgg ccgcaggtcg ccgggaaccc ccatgacgtg cccgcgcggc atcggccgca
    11101 gcgcgccgcg ggtccacagg tgggcggtgg cggtggcggg cggctggagg gccgcgccga
    11161 ggcccacggc ccgggcgagg tccacggcct cggggcggcg ggcgagcacg gattcggcgc
    11221 cgaggtcgac ggggacgccg gcgagctcac cggcgcgcag cttgccgccg agcagcgggc
    11281 c
//
