LOCUS       NZ_CP102509            10405 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1414780
            Orig. end                         :: 1425185
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="11"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            295..495
                     /locus_tag="JIW86_RS07290"
                     /old_locus_tag="JIW86_07290"
     CDS             295..495
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735981.1"
                     /locus_tag="JIW86_RS07290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07290"
                     /product="hypothetical protein"
                     /protein_id="WP_257553030.1"
                     /transl_table=11
                     /translation="MGGSQDKRREPDKSREEQERLRRPSQDPVHPDQGPPPRGKGPDEI
                     SRRRGEHPLQEERDLRDDEDF"
     gene            974..>1084
                     /locus_tag="JIW86_RS41630"
                     /pseudo=""
     CDS             974..>1084
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /locus_tag="JIW86_RS41630"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="diacylglycerol kinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNPRSGGGKVGRFGLAEKAEALGARVVLLDTAVRTDV"
     gene            1209..1592
                     /locus_tag="JIW86_RS07300"
                     /old_locus_tag="JIW86_07300"
     CDS             1209..1592
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS07300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_07300"
                     /product="hypothetical protein"
                     /protein_id="WP_257553032.1"
                     /transl_table=11
                     /translation="MGGRRTRLDLGVLGVVGIRVNNAAQAADVALRGARAAGLQVLTSQ
                     QVVIGSDTDSIPVAVDGEALTMPTPVTCSIRPGALRVLVPRIRPGAPAAGPPMHWREVV
                     SLAFSRRRPGRAAEDRADAERAP"
     gene            1701..1928
                     /locus_tag="JIW86_RS07305"
                     /old_locus_tag="JIW86_07305"
     CDS             1701..1928
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /locus_tag="JIW86_RS07305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07305"
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="WP_215150726.1"
                     /transl_table=11
                     /translation="MKDLKFEQKRSLSRLEAADQLSALAEALRHGGNAELELGPGTLTL
                     RIPDELRTEIEVEVGDGEIEMEIELKWPTA"
     gene            complement(1944..2738)
                     /gene="ppk2"
                     /locus_tag="JIW86_RS07310"
                     /old_locus_tag="JIW86_07310"
     CDS             complement(1944..2738)
                     /EC_number="2.7.4.1"
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ppk2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670118.1"
                     /locus_tag="JIW86_RS07310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07310"
                     /product="polyphosphate kinase 2"
                     /protein_id="WP_257553033.1"
                     /transl_table=11
                     /translation="MGLKKAVYEKELLRLQTELIKLQEWVRVEGARLVVVFEGRDAAGK
                     GGTIKRVAEHLNPRVARIAALPKPTERERTQWYFQRYVEHLPAAGEIVLFDRSWYNRAG
                     VEHVMGFCTPAEHQRFLRQCPVFEHMLVEDGILLRKYWFSVSDAVQEERFRRRTQDPLR
                     RWKLSPMDLESLTRWEAYSRAKDEMLVHTDTVDAPWYVVESDDKRSARLNMIAHLLSSV
                     SYEDVALPSLVLPPRPPSTGYERPPKELQTAVPDHAAGLRKA"
     misc_feature    2886..2903
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.88"
                     /tool="antismash"
     misc_feature    2933..2946
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.1"
                     /tool="antismash"
     misc_feature    2933..2947
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.92"
                     /tool="antismash"
     misc_feature    3033..3045
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3070..3630
                     /gene="ectA"
                     /locus_tag="JIW86_RS07315"
                     /old_locus_tag="JIW86_07315"
     CDS             3070..3630
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141822.1"
                     /locus_tag="JIW86_RS07315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07315"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_257553034.1"
                     /transl_table=11
                     /translation="MTAHAELARYGKILPSAPTTPTNPTTHIDRPRPGDGPLLWRLARD
                     SRTLDLNSAYSYLLWCRDFAGTSAVARDASGRAVGFVSGYLRPDSPGTLLVWQIAVDAS
                     ARGLGIAGALLDGLSARVAAEHGLSSLETTISPGNLASERLFASYAARHGATLTREVLF
                     TAEEFPDGGHAPEVLHRIGPLHF"
     misc_feature    3655..3670
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     gene            3696..4964
                     /gene="ectB"
                     /locus_tag="JIW86_RS07320"
                     /old_locus_tag="JIW86_07320"
     CDS             3696..4964
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.1e-71.
                     Score: 231.7. Matches aSDomain:
                     nrpspksdomains_JIW86_RS07320_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="JIW86_RS07320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07320"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_257553035.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.8e-74, bitscore:
                     249.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMTEPVLSVFETVESEVRSYCRAWPVVFERATGSRLYDEHGRGY
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGITHGLDMATTAKRAFLETFRSHVLEPRA
                     LPYKVMFPGPTGTNAVEAALKLARKAKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLGGRVPDFLWFERLLEDQGSGLNHPAAVIVETIQGEGGINVARA
                     EWLRALADLCRRRDMLLIVDDIQMGCGRTGEFFSFEEAGITPDIVTLSKSISGYGLPMS
                     LCLFKPELDLWEPGEHNGTFRGNNPAFVTATAALETYWGDGVLRERTLVQGGRIESALR
                     ELCGEHGQLGATYRGRGLVWGIEFAHKERAAEVCRRAFEHGLLVETSGPESEVVKLLPP
                     LTVTGDELDEGLAILARSVREAA"
     aSDomain        3780..4790
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JIW86_RS07320_Aminotran_3.1"
                     /evalue="4.10E-71"
                     /label="JIW86_RS07320_Aminotran_3.1"
                     /locus_tag="JIW86_RS07320"
                     /protein_end="365"
                     /protein_start="28"
                     /score="231.7"
                     /tool="antismash"
                     /translation="FERATGSRLYDEHGRGYLDFFAGAGSLNYGHNNPVLKRALLDYLE
                     RDGITHGLDMATTAKRAFLETFRSHVLEPRALPYKVMFPGPTGTNAVEAALKLARKAKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLGGRVPDFLWFER
                     LLEDQGSGLNHPAAVIVETIQGEGGINVARAEWLRALADLCRRRDMLLIVDDIQMGCGR
                     TGEFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFKPELDLWEPGEHNGTFRGNNPAFV
                     TATAALETYWGDGVLRERTLVQGGRIESALRELCGEHGQLGATYRGRGLVWGIEFA"
     gene            5001..5405
                     /locus_tag="JIW86_RS07325"
                     /old_locus_tag="JIW86_07325"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536875.1"
                     /locus_tag="JIW86_RS07325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07325"
                     /product="ectoine synthase"
                     /protein_id="WP_257553036.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.1e-53, bitscore:
                     176.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFRDIEGTDRHVRATSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVVCTAGEAELTDEETGRTYTITPGTMYLLDGHERHTLRVKHEFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDHS"
     gene            5482..6384
                     /gene="thpD"
                     /locus_tag="JIW86_RS07330"
                     /old_locus_tag="JIW86_07330"
     CDS             5482..6384
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="JIW86_RS07330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07330"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_257553037.1"
                     /sec_met_domain="PhyH (E-value: 8.8e-38, bitscore: 129.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYASTRTANDLYPTRGSEEVLTGRTDPVVWSEPGTPGPIWAHEL
                     ESFERDGYLSVDELITPDEVDVYRAEMDRLLRDPSTLADERSIVEPKSREVRSVFEVHR
                     ISEVFASLAADPRVVGRARQILGSEVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGL
                     PHMRTVSVSIALTPNYTTNGGLMIMPGSHRSFLGCAGATPKDNYKKSLQMQDAGTPSDE
                     ALTKFASACGIKLFTGKAGSATWFDCNAMHGSGDNITPHPRSNVFLVFNSVENRPSKPF
                     AAPVRRPGYIASQDFTPVR"
     gene            6856..8331
                     /locus_tag="JIW86_RS07335"
                     /old_locus_tag="JIW86_07335"
     CDS             6856..8331
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029980.1"
                     /locus_tag="JIW86_RS07335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_07335"
                     /product="FAD binding domain-containing protein"
                     /protein_id="WP_257553038.1"
                     /transl_table=11
                     /translation="MVAARITVNGKEAPISPAAPHTTVLDFLRERGLTGTKEGCAEGEC
                     GACSVLVARPGVHKPTDWVAVNACLVPVAALDGQEVVTSEGLATAGEPGMPATLHPVQE
                     EMAVRGGSQCGYCTPGFICSMASEYYRPGRCAHADSDAASADSTDSEHGPNGFDLHALS
                     GNLCRCTGYRPIRDAAFAVGAPAEDDALAQRREQSPPEPAATEYTQDDSAFLRPSTLAD
                     ALRLLRERPGAVVVAGSTDWGVEVNIRSRRANCVVAVDRLPEMRLLRVESDHIEIGAAL
                     TLTEIERRLDGSVPLLAELFPQFASRLIRNSATLGGNLGTGSPIGDSPPVLLALEASVV
                     LADADGEREVPLSDYFTGYRQSVRRPGELIRAVRVPLPLSPVVAFHKIAKRRFDDISSV
                     AVAFALDIEDGIVRKARIGLGGVAATPIRAIAAEAALEGKPWAVETVEGAARVLRAQGT
                     PMDDHRASSGYRSAMLGQSLLKLYAQTTEAVSS"
     misc_feature    9729..9742
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 tccccctgcc ctggcgggca acggaacccg cctctccccg gctcggctct gtagggccat
       61 catctcccac atccccggac acagccgtgc gggacgcaga cccgggtccg atacgtgcct
      121 tgacctcggc attcgcacga ccccgacaac ccggaacggc cgtaccgcag ggcacagtac
      181 gtccctctcg cgtcactccc ccgccacgat tcgcggtcga cgggatgcgc atcgtgacgg
      241 accgcgtgag tgtgggagca gaaggccttc acacgcatcg aaggagtcac cctcatgggc
      301 ggtagccagg acaagcgccg tgagccggac aagagccgcg aggaacagga gcggctgcgg
      361 cgccctagcc aggaccccgt ccaccccgac cagggcccgc caccccgtgg aaagggcccc
      421 gacgagatca gccggcgccg cggggaacac ccgctccagg aagagcgcga cctgcgtgac
      481 gacgaggact tctgaaccgg agcaccgcgg tcccacgtac gcagagcccc ggccggacac
      541 agccaccccg gccggggctc tgccatgact ctccgatcgg ccgtccgctc cccggagtcg
      601 ggcacggctg tcagccgggc tggccttggc ctgcgcactg ggcgcggtgg tggcactggt
      661 ggctgcgagc ggccccggcg gtctgttcat cctcgtggtc ggggtggtcg gcgcggcgct
      721 cgcggccgtg ggtacgtggt gggtgctgtc ccaccacggc gcggtgcggg tggtcggagc
      781 actgatcatg acgggcgcac cgctggccgt cctggtcatc tacgcccggg cggatctgtg
      841 gcccgtggcg ctgaccgcca tcgcgctgtg gggagccgcc gtggtgtgcg cgcgggcggc
      901 gctgcgcggg gtgcgacaac ccgagggaat gcaggcggtc gcctgcgccc tcccccggca
      961 tccggtgctg atcatgaacc cgaggtccgg cggtggcaag gtcggccggt tcggcctggc
     1021 cgagaaggcc gaggcgctgg gtgctcgggt cgtgctgctg gacaccgcgg tcaggacgga
     1081 cgtggccgaa atcgcccgtc aggcggtggc cgaaggcgcg gacctgctcg gggtggcggg
     1141 cgggcaccgg gccgggtcct tgtggtccag gccgagaacc ttggcgcgcc gcccccgaac
     1201 cgatgaccat gggcggccgc agaacccggc tcgacctcgg agtgctggga gtcgtcggga
     1261 tccgggtgaa caacgcggcc caggcggccg acgtcgcgct ccgaggtgcc cgggccgccg
     1321 gcctccaggt actgacctcc cagcaggtcg tcatcggatc ggacaccgac agcatcccgg
     1381 tcgccgtgga cggagaggcc ctgaccatgc ccacacccgt cacctgctcc atccgcccgg
     1441 gtgcgttgcg cgtactggta ccgaggattc gccccggggc tccggcggcc ggtccgccca
     1501 tgcactggcg tgaggtcgtg tcactggcct tcagccggcg gcggccgggg cgcgcagcgg
     1561 aggaccgggc cgacgcggag cgtgccccgt gactcgcccg gcccgaggat gcccgacctg
     1621 ccgcgcagcg tcacaatgag ccttaccgct gctttcgctg ttcccgctgt ttcgctgttc
     1681 gtagcggatt ggagacatcc gtgaaggacc tcaagtttga gcagaagcgt tccctgtccc
     1741 gcctcgaggc cgccgaccag ctctccgcgc tcgcggaggc gctgaggcac ggcggcaatg
     1801 ccgaactgga gctcggtccc gggaccctga ccctgcggat cccggacgaa ctccgcaccg
     1861 agatcgaggt cgaggtcggc gacggggaga tcgagatgga gatcgaactc aagtggccca
     1921 ccgcctgagt tcaggggcgg gcatcaggct ttccgaagac cggccgcgtg atcgggtacg
     1981 gcggtctgaa gctccttggg cgggcgctcg tatcccgtcg acgggggccg cggcggcagg
     2041 acgagcgacg gcagggccac gtcctcgtag gagaccgatg acagcaggtg ggcgatcatg
     2101 ttcagtctcg cgctgcgctt gtcatcgctc tcgaccacgt accagggggc gtccaccgtg
     2161 tccgtgtgga cgagcatctc gtccttggcc cgggagtacg cctcccagcg cgtgagggac
     2221 tccaggtcca tcggcgagag cttccaccgc cgcaacgggt cctgcgtgcg gcggcgaaag
     2281 cgttcctcct ggacggcatc gctgacggag aaccagtact tgcggagcag gatcccgtct
     2341 tccaccagca tgtgctcgaa gaccgggcac tggcgcagga agcgctgatg ctcggcgggc
     2401 gtacagaagc ccatgacgtg ttccactccg gcgcggttgt accagctgcg gtcgaagagc
     2461 acgatctcac cggcggccgg caggtgctcc acgtagcgct ggaagtacca ctgggtgcgc
     2521 tcccgctccg tcggcttcgg aagggccgcg atacgtgcca cgcgggggtt gaggtgttct
     2581 gcgacgcgtt tgatcgttcc gcctttgccc gctgcgtccc ggccttcgaa gacgacgacc
     2641 agtcgggcac cctccacacg cacccattcc tggagcttga tcagctccgt ctggagccgc
     2701 agcagttcct tctcgtagac cgccttcttg agacccatgg ccgtctcctc cccgtcgtag
     2761 tccggcgcga aatccgtcgc gccggccagg cgcgccgtcg cgtataaccg tgcagctatg
     2821 aacgaaaccc ccgaccggcc gacgaagaca tcacattcaa gtggaatata tgagacagaa
     2881 caaagacgtt atccgctcgt tatagaagcg ggggtggggt ggaaatatca gcatttttca
     2941 tagaactacg gggcacttgc cagcgtctcg cgatcccagg aagccgtctg caactatcac
     3001 gagtcatgcc agttccataa cccctcaggg ccatgcaatt tgcgccatcg ctgggtaact
     3061 tcggcccgca tgactgctca tgcagaacta gcccgttacg gaaagatcct gccgtccgct
     3121 ccgaccaccc cgaccaaccc caccacgcac atcgaccgcc cccgcccagg tgacggcccc
     3181 ctgctgtggc gcctcgcccg cgactccagg acgctggacc tcaactcggc ctacagctat
     3241 ctcctctggt gccgtgactt cgccggcacg tccgcggtgg cccgcgacgc ctccgggcgt
     3301 gccgtcggat tcgtcagcgg atacctgcgg cccgacagtc ccggaaccct gctcgtctgg
     3361 cagatcgccg tcgacgcctc cgcccgcggc ctgggaatcg ccggggccct gctcgacggc
     3421 ctctccgccc gcgtggcggc agaacacgga ttgagctccc tggagaccac catctccccc
     3481 ggaaacctcg cgtcggagcg actgttcgcc tcgtacgccg cacggcacgg cgccacgctc
     3541 acgcgcgaag tgctgttcac cgcggaagag ttcccggacg gcgggcacgc cccggaggtc
     3601 ctgcaccgca tcgggccgct ccacttctga tcccggttct gatcccggtt cgcacagccg
     3661 ataccgaaca cccccacatt cattggagag ttgccttgac catgaccgag ccggtcctgt
     3721 ccgtcttcga gaccgtggag tccgaggtgc gaagctactg ccgcgcctgg cccgtcgtct
     3781 tcgagcgcgc cacgggaagc cggctgtacg acgaacacgg ccgcggctac ctggacttct
     3841 tcgcgggcgc cgggtccctc aactacggcc acaacaaccc cgtcctgaag cgggccctgc
     3901 tcgactacct ggagcgggac ggcatcacgc acgggctgga catggcgacg accgccaaga
     3961 gggccttcct cgagacgttc cggtcccacg tcctcgaacc ccgcgcactg ccctacaagg
     4021 tgatgttccc gggacccacc gggaccaacg ccgtcgaagc ggcgctcaag ctggcgcgga
     4081 aggccaaggg ccgcgagtcg gtcgtgtcct tcaccaacgc cttccacggc atgtcgctcg
     4141 gctcgctcgc cgtcacgggc aacgccttca aacgggccgg cgcaggcatc ccgctggtgc
     4201 acggcacgcc gatgcccttc gacaactacc tcggcggccg ggtccccgac ttcctctggt
     4261 tcgagcggct gctggaggac cagggctccg gcctcaacca cccggcggcg gtcatcgtcg
     4321 agacgatcca gggcgagggc gggatcaacg tggcccgcgc ggaatggctc cgcgccctcg
     4381 cggacctgtg ccggcgccgc gacatgctgc tcatcgtcga cgacatccag atgggctgcg
     4441 gccggaccgg cgagttcttc tccttcgagg aggccggcat caccccggac atcgtcacgc
     4501 tgtccaagtc catcagcggc tacggactcc ccatgtccct gtgcctgttc aagcccgagc
     4561 tggacctctg ggagccgggc gagcacaacg gcaccttccg cggcaacaac ccggccttcg
     4621 tcaccgccac cgcggccctg gagacgtact ggggcgacgg cgtcctgcgc gagcggaccc
     4681 tcgtccaggg cgggcgcatc gagagcgcgc tgcgcgagct gtgcggcgaa cacggccagc
     4741 tgggcgcgac ctaccggggc cgcggactgg tctggggcat cgaattcgcc cacaaggagc
     4801 gcgcggcgga ggtctgccgg agggccttcg agcacggtct cctcgtcgag acctcgggcc
     4861 ccgagagcga ggtcgtgaag ctgctcccgc cgctcaccgt caccggcgac gaactcgacg
     4921 agggccttgc catcctcgcc cgctccgtcc gcgaagccgc ctgaccggca cccgcccccc
     4981 acaccacgaa gggcaccacc atgatcgtcc gctcgttccg ggacatcgag ggaaccgacc
     5041 gccacgtcag ggcgacatcc ggcacctggg agagcaagcg catcgtcctg gccaaggaac
     5101 gcgtcggctt ctccctgcac gagaccgtcc tgtacgccgg caccgagacg tccatgtggt
     5161 acgcgaacca tgtggaggcc gtggtctgca cggcggggga ggctgaactc acggacgagg
     5221 agacgggccg gacttacacg atcacgccgg gaaccatgta cctcctcgac ggccacgagc
     5281 ggcacaccct gcgcgtgaag cacgagttcc gctgcctgtg cgtcttcaac ccgcccgtga
     5341 ccggccggga ggaccacgac gagaacggcg tgtacccgct gctcaccgaa cccgaccaca
     5401 gctgacctgc ggaaacaccc agaaagaaag cacgcgagaa agcccagaga gaaagcacgg
     5461 agaggagagg caggcaccac catgacctac gcatccaccc gcactgcgaa cgacctgtat
     5521 ccgaccaggg gctccgagga ggtgctcacc ggccgcaccg accccgtcgt ctggtccgag
     5581 cccggcaccc cgggccccat ctgggcccac gagctggaga gcttcgagcg cgacggctac
     5641 ctgtccgtcg acgaactcat cacgcccgac gaggtggacg tctaccgggc cgagatggac
     5701 cggctgctgc gcgatccttc gacgctggcc gacgagcggt ccatcgtgga gccgaagtcc
     5761 cgggaagtac gctccgtctt cgaagtccac cggatcagcg aggtgttcgc gtccctcgcc
     5821 gccgatccgc gtgtggtggg gcgcgcgcga cagatcctgg gctccgaggt gtatgtgcac
     5881 cagtcccgga tcaacgtaaa gcccggtttc ggcgccagcg gcttctactg gcactccgac
     5941 ttcgagacct ggcacgccga ggacggcctg ccgcacatgc gcaccgtgtc ggtgtccatc
     6001 gccctgacgc cgaactacac caccaacggc ggcctgatga tcatgcccgg ctcccaccgg
     6061 agcttcctgg gctgtgccgg tgcgacgccg aaggacaact acaagaagtc cctccagatg
     6121 caggatgcgg gcaccccctc ggacgaggcg ctgaccaagt tcgcctcggc ctgcggcatc
     6181 aagctcttca ccggcaaggc gggttcggcc acctggttcg actgcaacgc gatgcacggc
     6241 tcgggtgaca acatcacgcc ccacccgcgc agcaacgtgt tcctcgtctt caacagcgtc
     6301 gagaaccgcc cgtcgaagcc cttcgccgcc ccggtgcgcc gccccgggta catcgcctcg
     6361 caggacttca cccccgtgcg ctgacgcgtc cggccgaccc ggcccggccc ggcatctgcc
     6421 cggccgggcc ggcctgtccc ccgcacccga ttggttgcgc gtacgaagac cggccgcacg
     6481 aagtggccgg tcttcgtgga atcgaccgac atcggcttga ccgatgaacc tgcggcaacg
     6541 taacttcggc cgcaccgatt cgcgagctac tggaaggagg cgaagccgga tgtcgaccat
     6601 ctccgagctg tctcgcctcg ctcaccagat cgcctgggct ccgggtcgtg tagcacacgg
     6661 ctgctgagca gcccctcttg ccggcgtgcc cgatcgcggc cccggcacct ttccatctct
     6721 gtcaccgttt acggcacgtc gttgtgtgcc gatttattgc gctctgggtt ctcgagctgc
     6781 gtcaccgaaa tagaaagctc ctgaaatggc gactaggaca gtcagatcga ccgcgcgccg
     6841 aaatgaggac gtgccatggt agcggcgcgg atcacggtca acgggaaaga ggcaccgatt
     6901 tcaccggctg caccccacac cacggtgctg gatttcctgc gcgagcgcgg cctcaccggc
     6961 accaaggagg gctgcgccga gggtgaatgc ggcgcctgct cggtcctggt ggcccgtccc
     7021 ggggtgcaca agcccaccga ctgggtggcg gtcaacgcct gcctggtccc ggtcgcggcg
     7081 ctcgacggtc aggaggtcgt cacctccgaa ggtctcgcca ccgccggcga acccggtatg
     7141 ccggccacct tgcaccctgt gcaggaggag atggccgtcc gcggcggttc ccaatgcggt
     7201 tactgcacac cggggttcat ctgcagcatg gcctccgagt actaccgccc cggccgctgc
     7261 gcacacgcgg actcggacgc ggcctcggcc gacagcaccg actccgaaca cggtccgaac
     7321 ggtttcgacc tgcacgcgct gagcggaaac ctgtgccgct gcaccggcta tcgcccgatt
     7381 cgcgatgccg cgttcgccgt cggtgcgccc gccgaggacg acgccttggc gcagcgccgc
     7441 gagcagtccc cgcccgaacc ggccgccacc gaatacaccc aggacgacag cgcgttcctg
     7501 cggccgagca ccctggccga tgcgctgcgg ctgctgcgcg agcggcccgg cgcggtggtg
     7561 gtcgccggaa gcaccgactg gggcgtggag gtgaacatcc gttcccgcag ggcgaattgc
     7621 gtggtcgcgg tcgaccggct gcccgaaatg cggttgctgc gagtcgaatc cgaccacatc
     7681 gagatcgggg cggcgctgac gctcacagag atcgaacgcc gcctcgacgg cagcgttcca
     7741 ctgctggcag agctgttccc gcagttcgca tcccggctca tccgcaacag tgcgaccctc
     7801 ggcggcaacc tgggtaccgg gtcccccatc ggtgacagtc cgccggtgct gctcgcgctc
     7861 gaggcgtcgg tggtactcgc cgacgccgac ggtgagcgcg aagtccccct gtccgactat
     7921 ttcaccggct accggcagag cgtgcgccgt cccggcgagc tgatccgcgc ggtacgcgtc
     7981 ccgctgccgc tgtcgccggt cgtggccttc cacaagatcg ccaaacggcg cttcgacgac
     8041 atctccagcg tggcggtcgc gttcgcgctc gacatcgagg acgggatcgt acgcaaggca
     8101 cgcatcggcc tgggcggcgt ggccgccacc ccgatccgcg ccatcgccgc cgaggcggcc
     8161 ctggagggca agccgtgggc ggtggagacc gtcgagggcg cggcccgggt gctgcgggcc
     8221 cagggcacgc cgatggacga tcaccgcgcc agctccggct accgctccgc gatgctcggc
     8281 cagagcctgc tgaagctgta cgcacaaacc accgaggcgg tgtcgtcatg agccatttgt
     8341 ccgagcgccc cgaaatgccg gtcgtcggcg tttcgatgcc acacgagagc gccaacctgc
     8401 acgtcaccgg caccgcgctc tacaccgacg acctggtcca tcgcaccaag gacgtgctgc
     8461 acgcctaccc ggtccaggtc atgaaggccc acggcaggat cacggcgctg cgcaccgggc
     8521 ccgcgctcgc cgtgcccggt gtggtccgcg tgctgaccgg cgccgacgta cccggtgtga
     8581 acgatgccgg aatgaagcac gacgaaccgc tgttccccga cgaggtcatg ttctacggcc
     8641 acgcggtcgc ctgggtgctc gccgagaccc tggaggcggc ccggctcggt gcggcggccg
     8701 tcgaggtgga actcgacgaa cagccctccc tgatcacgct gcaggacgcc atcgcggcgg
     8761 acagttttca cggcgcccgg cccgtgatgc tcgccggcga cgtcgacgcc ggtttcggcg
     8821 actccgcgca cgtgttcacc ggcgagttcc agttctccga tcaagagcac ttctacctcg
     8881 agacgcacgc ggcgctggcc cacgtcgacg agggcgggca gatgttcgtc cagagcagca
     8941 ctcagcatcc ctcggagacg caggagatcg tcgcgcacgt gctcggcctg cacagccacg
     9001 aggtgaccgt gcagtgcctg cggatgggtg gcggcttcgg cggcaaggag atgcagccgc
     9061 acgggttcgc ggccatcgcc gcgctcggcg ccaggctgac cggccggccg gtgcgcttgc
     9121 ggctcaaccg gacccaggac ctgaccatgt ccggtaagcg ccacgggttc cacgccgagt
     9181 ggaagatcgg cttcgacgcc gacggccgca tccaggccct ggacgccacc ttgaccgcgg
     9241 acggcggctg gagcctggac ctgtccgagc cggtggtggc ccgcgcgctg tgccacatcg
     9301 acaacaccta ctggattccc aacgcgcgta tcgccggtcg catcgccaag accaacaagg
     9361 tctccaacac cgccttccgc ggcttcggcg gaccgcaggg catgctggtg atcgaggaca
     9421 tcatgggccg gtgcgcgccg ctgctcggcc tggatccgat ggagctgcgc gagcgcaact
     9481 tctaccggca gggccattcg acgccgtacg ggcagccggt cctccacccc gagcggatct
     9541 cgaccatctg gcagcaggtt caggaagacg ccgccatcgc cgatcgcaag ctcgagatcg
     9601 cggccttcaa caccgcgcac ccgaacacca agcgggcgct cgcgatcacc gggatcaagt
     9661 tcggaatctc gttcaacctc accgccttca accaggcggg cgcgctggtg ctgatctaca
     9721 aggacggctc ggtcctgatc aaccacggcg gcaccgagat ggggcagggc ctgcacacca
     9781 agatgctgca ggtggccgcg accactctgg gtatcccgct gcacaaggtg cggctggccc
     9841 cgacgcgcac ggacaaggtt cccaacacct ccgccaccgc cgcaagttcc ggggcggatc
     9901 tcaacggtgc ggcggtgaag aacgcctgcg agcagttgcg cgagcggctg ctgcaggtcg
     9961 cgggcaccca actgggtgtg agcgcctcgg acgtgcgcat cgtcgagggc gtcgcgcggg
    10021 ccctgggcaa cgacaaggag ctggcctggg acgacctggt gcgcaccgcg tacttccagc
    10081 gagtccagct gtcggcggcc ggtttctacc ggaccgaggg tctgcactgg gacgcgaaga
    10141 ctttccaagg ctccccgttc aagtacttct cctacggcgc cgccgctacc gaggtggagg
    10201 tggacggctt caccggcgcg taccgcatcc ggcgggtgga catcgtgcac gatgtcggcg
    10261 acagcctctc cccgatgatc gacatcggtc aggtcgaggg cggtttcgtg cagggcgcgg
    10321 gctggctgac gctggaggac ctgcgctggg acagcggtga cgggccgaac cgcggccggc
    10381 tgctgaccca ggccgcgagc accta
//
