LOCUS       NZ_CP102509            20708 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 985685
            Orig. end                         :: 1006393
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20708
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="4"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20708
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20708
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10708](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10708
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(245..4173)
                     /gene="fxsT"
                     /locus_tag="JIW86_RS05325"
                     /old_locus_tag="JIW86_05325"
                     /pseudo=""
     CDS             complement(245..4173)
                     /codon_start=1
                     /gene="fxsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743959.1"
                     /locus_tag="JIW86_RS05325"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05325"
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTVSRDSDSRDGRIVTFYSYKGGTGRTMALANTAWILAANGKRVL
                     AVDWDLEAPGLHRFFHPFLDPSTLGATTGVIELISEYAWAATSPVQRADAWHEDYARIQ
                     PHAVSLTPEALGWEFPDGGALDFVSAGRQNREYSATVSTFDWDNFYDRLGGGLFFDALR
                     ADMKRNYDYVLIDSRTGLSDTADICTVHLPDLLVDCFTLSDQSIDGAAAVARQIDERFN
                     DRGITIYPVPMRVDESDPDKADAGRALARTTFDRFPHGLVGDELTSYWRAVEIPYRSSY
                     AYEEILATFGDEAGLSDSLLSAFERLTAVITEGEVTSMPAMEEQTRLRIRDAFTRRPPG
                     LPTDLFLSYVPENRMWADWAESVLTRAGCRVIRMDASAGDPPAGADMSGDTATRTVVLL
                     SGAYLNSARAVDVWERAAAVDPTGGRRRLIPLRVGDLRLSTPYIDHDPVDLFRLDEVHA
                     TAALLRAVDRPMALPDRVAGSSQPAPRFPGAIPKVWNAPPRNPGFTGRHTVLERMRDQL
                     GGGMAVVLPQPQTLYGLGGVGKTQVVLEYAHRFMADYDLVWWIPSEETDEIVAALAELA
                     ERLGAQTGEDMSAASQEAIDLLRRGFRPRGGCWSSTTPTIRRPSSGSSRRADRGTYS"
     misc_feature    complement(3688..3690)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4148..4160
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.96"
                     /tool="antismash"
     gene            complement(4255..5253)
                     /locus_tag="JIW86_RS05330"
                     /old_locus_tag="JIW86_05330"
     CDS             complement(4255..5253)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102828.1"
                     /locus_tag="JIW86_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05330"
                     /product="MASE1 domain-containing protein"
                     /protein_id="WP_257552739.1"
                     /transl_table=11
                     /translation="MRTEGFRRLCMTVLLNLIVAAAYFAGGRIGLLQQVVIQGAVVTPL
                     WPPTGIALVCLLWMGLRVWPGIALGACLVIQSISGSIDPVGFGIVAGNTLAPVCAYLML
                     RRVGFRVTLDRLRDGVALVFLGGLVPMLISATIGAWLLVVTDSLPDSGFWIVWWTWWAG
                     DAMGVLVIAPLLLVLRMARLPSGAYRWAEAAALLVTAFAVTLLATRSDMALLFLVFPLL
                     IWAALRFQLEGSAPCMLLVSAMANSAAIAQEGPFDHHTLVEAMVNLQALNGSAALTSLL
                     LSAIVTEQNIIRARIEQACQDLAEVVERLAPGKATSGWPPRDEDGVETGGG"
     gene            complement(5319..6152)
                     /locus_tag="JIW86_RS05335"
                     /old_locus_tag="JIW86_05335"
     CDS             complement(5319..6152)
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459354.1"
                     /locus_tag="JIW86_RS05335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05335"
                     /product="PP2C family protein-serine/threonine phosphatase"
                     /protein_id="WP_215141072.1"
                     /transl_table=11
                     /translation="MSHHRSTRSATADDLLTRLGRLTAQAREGAELQQARVELAEALQR
                     EILPASLPGVPGLRTAARYAPARHGLSIGGDWYDGFLLPEGALGFSIGDVEGHDVEAAA
                     FMGQVRIAVRAVAASAADPGEVLSRANDLLLSVDCDLFATCTFLRFDPITWEIQTARAG
                     HVPGVWATTDGEHGIVEDEGGLPLGMLPGSQYPVTRRRLATGGSFVLVTDGVVEGPSFP
                     IDRGLARVAEVVRAKAGADPGELAAEVMRVADSTGHEDDAAVLVLRHEAVRGGTG"
     misc_feature    6207..6221
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.54"
                     /tool="antismash"
     gene            complement(6249..8450)
                     /locus_tag="JIW86_RS05340"
                     /old_locus_tag="JIW86_05340"
     CDS             complement(6249..8450)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134889.1"
                     /locus_tag="JIW86_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05340"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_257552740.1"
                     /transl_table=11
                     /translation="MTRTTGSRLTAFVAAAVSVLTVVQPTQAATPQASAQAAAALPTGW
                     STVVNAGSGKCLDARSAATANGTAVQQYTCNNSSAQQWSFTPTTDGFVRIGNRNDAAQV
                     VDVSDTSSADNAAVHLWTYGGGANQQWRAVDEGGGAYHFVSRHSGKCLDVPSASTADSV
                     QLVQYTCNGTAAQRFQVAPVNTAPGDVDLGPNVVVFDPSMPSSTIQSRLDSIFRQQETN
                     QFGAQRYAVMFKPGTYANDVNVGFYTQVLGLGQSPDSVTINGAVHVEADWFPPHNATQN
                     FWRGAENLSVNPTGGTDRWAVSQAAPYRRMHVRGNLALDDGGWASGGFMADSKIDGQVR
                     SGTQQQWLTRNSQLGGWTGSNWNMVFVGSQGVPATSFPNPPYTTVNQTPTVREKPFLYV
                     DGTGAYKVFVPSLRTDSSATSWANGNAAGSSLGMDQFYVVKPGASAAQIDAALAEGKNL
                     LVTPGVYHLDRTLRVTRPDTVVLGLGLATFIPDNGVTAMTVADVDGVKIAGVLFDAGTT
                     TSRTLMEVGPAGAAANHSANPTSLHDVFFRVGGAAVGKATTSLVVNSDHVIGDHMWIWR
                     GDHGSGIGWNSNTADTGLVVNGDGVTMYGLFVEHYQKHQTVWNGNGGRTYFYQNEMPYD
                     PPNQAAWMNGSTQGYAAYKVADHVTSHQAFGLGSYCFFNVNSSVTAERAFEAPGNPNVR
                     FQSMVTVSLGGTGTIRHVINDRGGPSNASTNVANLVSYP"
     gene            complement(8447..9733)
                     /locus_tag="JIW86_RS05345"
                     /old_locus_tag="JIW86_05345"
     CDS             complement(8447..9733)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152519.1"
                     /locus_tag="JIW86_RS05345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05345"
                     /product="family 16 glycosylhydrolase"
                     /protein_id="WP_257552741.1"
                     /transl_table=11
                     /translation="MNFPHLSRRLLLSAVSLLALASLSTGLAQGVPPLHAPAPAAVAAE
                     GAASAAAVTFSDEFNGPAGSAVDGAKWQIETGDNVNNHERQFYTSGNRNAALDGQGNLV
                     ITARRENPANYQCWYGRCEYTSARLNTAGRFTTTYGRVEARMKVPRGQGMWPAFWMLGN
                     DIGQVGWPASGEIDVMENVGFEPSTVHGTLHGPGYSGSGGIGAGYTLPGGQAFVDAFHT
                     FTVDWSPNAITWSVDGAVYQRRTPADLGGRQWVFDKPFFLILNLAVGGYWPGDPDGSTV
                     FPGQLLVDYVRVTSGGGQPGGGGPITGLGGKCVDVAGANSANGTPVQLYDCNGTAAQQW
                     TVGSDGTIRALGKCLDVASGGTADGTPVQLYDCNGTPAQQWAVPAARDVVNPQANKCLD
                     VAGNSSANGTRLQIWTCTGAANQKWTVTR"
     misc_feature    9843..9858
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.02"
                     /tool="antismash"
     misc_feature    complement(9948..9967)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 24.04"
                     /tool="antismash"
     gene            10001..10708
                     /locus_tag="JIW86_RS05350"
                     /old_locus_tag="JIW86_05350"
     CDS             10001..10708
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889609.1"
                     /locus_tag="JIW86_RS05350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05350"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_257552742.1"
                     /sec_met_domain="CDPS (E-value: 2e-86, bitscore: 288.1,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATEVFTVQPFTPHCQVIHTEGDHAVIGISPGNSYFSAQRVTD
                     LAHWGLRNFRQVDLIYTDMHVAEMYEALGYPADEARRKAVKNLRGVRAKVNNAAAEADP
                     TGTRLRARPMSSLTDIPAYRRLHTHLNTLLGTDPEFRETCNALVDAFLSSKVLGGKTAT
                     VRQREVCLEYVCAEAPLFLDTPAILDVPSSLNCYHQLLPMAELLYSRGSGLRASRNQGH
                     AVITPAEGESDAH"
     gene            10698..11882
                     /locus_tag="JIW86_RS05355"
                     /old_locus_tag="JIW86_05355"
     CDS             10698..11882
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003999282.1"
                     /locus_tag="JIW86_RS05355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05355"
                     /product="cytochrome P450"
                     /protein_id="WP_257552743.1"
                     /sec_met_domain="p450 (E-value: 7.9e-17, bitscore: 60.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTDTLLDFPFSARGDQLPHEVEQLRTEPVKRVRTIAGDEAWLVS
                     SYDLCKQVLEDPRFSLKDTSAPGVPRQYALTIPPEVVNNMGNITGAGLRKAVLKAINPK
                     TDGLTDWMRAQATTLVDSLLTEGAPVDLRTQFTSPYAEQLHCRILGIPGEDAPRLAASL
                     DIAFMNSACPITGAKLNWDRDMAYMVERLDDPTVTGLMAELAALRKDPDYAHLTDEMLA
                     TVGVTLFGAGVISTMGFLTMALVSLIQHPEVWQQIRQDPSKIPAAVDELLRINLSIGDG
                     LPRLALEDVTLGDVEVKKGELLLVLVEAANTDPDVYPDPHTVDIDRPNAGTHLSFGGGQ
                     HYCPATALGKRHTEIALEVLVEKMPGLQLAVPVEQLVWRTRFMKRLPERLPVLW"
     gene            complement(11953..13140)
                     /locus_tag="JIW86_RS05360"
                     /old_locus_tag="JIW86_05360"
     CDS             complement(11953..13140)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005474074.1"
                     /locus_tag="JIW86_RS05360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05360"
                     /product="MFS transporter"
                     /protein_id="WP_257552744.1"
                     /transl_table=11
                     /translation="MDGDRQGWRQCLLGAAVFAVCMAGTTLPTPLYGLYQEKFGFSELM
                     VTVVYAVYAFGVIGVLLLVGNASDAVGRQPVLLAGLGCAAASAVCFLCATGMGWLYAGR
                     LFSGLSAGLFTGAATAYVMELAPSGRAAKATLVATAANMGGLGCGPLLAGVLAQYAVAP
                     LYLPFAVHLALVAVSVAVLLRLAETVRERRPLSTVRPQRPGLPAEVRAVFVPAAIASFV
                     GFALFGVFTSVSPAFLAESLDVHNHAVSGLVVALAFFASTAGQLLVGRVGVTRSLPLGC
                     AGLLAGLALLAGALYWDLLSLVVLSALVGGAGQGLAFRGAMSAVTEASPVHQRAAVISS
                     LFVVAYAGISVPVIGVGLLAGPMGLEGAGLVFIACMAVLVSTAAVYLLRRPGRPG"
     gene            complement(13219..14676)
                     /locus_tag="JIW86_RS05365"
                     /old_locus_tag="JIW86_05365"
     CDS             complement(13219..14676)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010071125.1"
                     /locus_tag="JIW86_RS05365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05365"
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="WP_257552745.1"
                     /transl_table=11
                     /translation="MALSRRELLASGVTAAVGPLTPTGRDTATLTLRGTIAPGAPDWVY
                     LPLDVPPGTAELRVSYTYAKPPVPPGTPGNALDIGIFDQRGTGLGGHGFRGWSGGARSE
                     FFLRADAATPGYLPGPLEPGRWHVALGPYTMAPEGLTYEITATFTYGDPGTPAVPGYPP
                     QRARGRGRAWYRGDCHLHSVHSDGRRTPAELAALARAAGLDFINSSEHNTHAAHAAWGP
                     VAAADPGLLVLTGEEITTRTGHVVAVGTAPGTFTDWRYRARDQRFDRFAHHIRKAGGLV
                     VPAHPHATCIGCAWKFGFGEADAVEVWNGPWTPDDEVALASWDATLTAGRGQWLPVLAS
                     SDYHRDPDRVGSPQTVVLAEDLSREAILAGLRAGHSYAAESSAVSVTFDAVTARGAHAG
                     IGDRIRATADTEEVLIRLTVTGSGSYDLRLVTDQGVAFTAPAATSLEWRTTPSRTSYVR
                     AEARHPASAPPLPGAPAALTNPILLSA"
     gene            complement(14768..15349)
                     /locus_tag="JIW86_RS05370"
                     /old_locus_tag="JIW86_05370"
     CDS             complement(14768..15349)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101639.1"
                     /locus_tag="JIW86_RS05370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05370"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_257552746.1"
                     /transl_table=11
                     /translation="MTEEQAERMLAGMNEVIRAGEEMRKLRTEMIKVFVGFGWTQDKIA
                     RLTDMSQPAVSKQVTKYKTGDPLPPLELSLDQYDAPWLEGRLWGLAEEISETLDGAARC
                     TRTIDALARGRKRFTPRDVDELRRLVEEDLRLHRGQLTARHQAAYDQIGRALDVPAKAA
                     ATAAATGTASASVRRALAHRIQRDRLRGDS"
     gene            15466..16293
                     /locus_tag="JIW86_RS05375"
                     /old_locus_tag="JIW86_05375"
     CDS             15466..16293
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (21-259). E-value:
                     1.1e-30. Score: 99.0. Matches aSDomain:
                     nrpspksdomains_JIW86_RS05375_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158226.1"
                     /locus_tag="JIW86_RS05375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05375"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_257559224.1"
                     /sec_met_domain="PF00561 (E-value: 2e-24, bitscore: 85.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.8e-16, bitscore:
                     59.7, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTFSAADGTRLAYRTHGDGDPVVCLPGGPADSAYLGDLGGLSAH
                     RRLIVPDLRGTGRSAVPEDTSSYRCDRLVDDVEALRAHLGLPAMDLLAHSAGANLAAQY
                     AARFPHRVSRLALITPGTRAVGIEITGETRRELAQLRGNEPWFPAAFAALEAITEGTGS
                     DWDAIAPFFCGRWDDRARQHQAAGRPRNMEAVTVFAAEGAFDPETTRAALAAFAAPVLL
                     LAGEFDLNSPPRSTAEFAGLFPDASFVVQPGAGHYPWVDDADRFAATTAAFLG"
     aSDomain        15529..16242
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JIW86_RS05375_Abhydrolase_1.1"
                     /evalue="1.10E-30"
                     /label="JIW86_RS05375_Abhydrolase_1.1"
                     /locus_tag="JIW86_RS05375"
                     /protein_end="259"
                     /protein_start="21"
                     /score="99.0"
                     /tool="antismash"
                     /translation="PVVCLPGGPADSAYLGDLGGLSAHRRLIVPDLRGTGRSAVPEDTS
                     SYRCDRLVDDVEALRAHLGLPAMDLLAHSAGANLAAQYAARFPHRVSRLALITPGTRAV
                     GIEITGETRRELAQLRGNEPWFPAAFAALEAITEGTGSDWDAIAPFFCGRWDDRARQHQ
                     AAGRPRNMEAVTVFAAEGAFDPETTRAALAAFAAPVLLLAGEFDLNSPPRSTAEFAGLF
                     PDASFVVQPGAGHYPW"
     gene            16350..17303
                     /locus_tag="JIW86_RS05380"
                     /old_locus_tag="JIW86_05380"
     CDS             16350..17303
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016638277.1"
                     /locus_tag="JIW86_RS05380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05380"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_257552747.1"
                     /transl_table=11
                     /translation="MPPFTTVAAYAPPGVGMLAVGVVTEVFGPRAGLPRFDFALCADRP
                     GPVPTDLGVPLAVEYGLERMAVADLVVALPWAGFRTPLAPAVLEALSAAHERGALVAAH
                     CVGTYALAAAGLLDGRRATTHWRFAELLARRHPEVTVDPHALYVDEGRVVTGAGAAAGF
                     DLCLHLVRREHGAAAANAIARDLVLPSHREGGQAQYLAAPVPEDAQDTRLAEVLAWARE
                     HLHEQLPVAELARRAVMSKRSFARRFTTATGTTPHAWLVSLRLSRAEELLETTDLSVEE
                     IARAVGFASAAVLREQFVRRRGVPPRSYRRSFTRTP"
     misc_feature    complement(17406..17424)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.85"
                     /tool="antismash"
     gene            17506..18236
                     /locus_tag="JIW86_RS05385"
                     /old_locus_tag="JIW86_05385"
                     /pseudo=""
     CDS             17506..18236
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743471.1"
                     /locus_tag="JIW86_RS05385"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05385"
                     /product="VOC family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTGDLDSAQQFYGAVLGWHFRRTRLGDGFSIAFRDGAPVAGIGAL
                     AQRLALPAAWTPYFAVDDADVTAARIVERGATMAVGPSPSAPDAPRWPPTPTERSSGSG
                     RARSSPTGRSARAARPPGWNCAPVTPSPRPCSTVRSSSGPANGPAAARSPTNTTTSCCA
                     TGTTPWRASWAGPSRKPPIRRSDRAGTSISASPTSRRRWKWPPVSAVASSRPWTPRAPA
                     ARSPWPIPTARCSPWWPRRTG"
     gene            18272..18892
                     /locus_tag="JIW86_RS05390"
                     /old_locus_tag="JIW86_05390"
     CDS             18272..18892
                     /GO_function="GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716691.1"
                     /locus_tag="JIW86_RS05390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05390"
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_257552748.1"
                     /transl_table=11
                     /translation="MRKIIAGLFISLDGVVEAPDQWHFPYFNDEMGAAVDATLGSVDTM
                     LFGRKTYDSFAGAWPEREAAGGEDAAFAKILGDARKIVASNRPLDFSWRNSERLEGDLV
                     EAVTALKAEPGPGPIGMSGSVSVVRQLLAAGLLDELHLLLHPIAVRKGMRLFDEGEAPV
                     PLTLISSETFRTGVLNLHYAPAERVGQGTYEDAKTHLRRQPEG"
     gene            18981..20351
                     /locus_tag="JIW86_RS05395"
                     /old_locus_tag="JIW86_05395"
     CDS             18981..20351
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562557.1"
                     /locus_tag="JIW86_RS05395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_05395"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_257552749.1"
                     /transl_table=11
                     /translation="MGKPSIDQLRERARGAVVTPQDDGYDEARAVNNAMIDRRPAAVVR
                     CANAGDVMATVDFARENGLDLAVRGGGHSVPGFGTCDDGVVADLSAMRSVRVDPVRRTA
                     RAEGGATWGDFNAATHAFGLAATGGIISTTGVGGLTLGGGIGYLARGLGLSCDNLLSAD
                     VVTADGRLLIASEEENPDLFWALRGGGGNFGAVTSFEFRLSPVKDIYGGPILYELEDAR
                     TVLRAFRELIADAPEQLGGFPAFQIAPPLPFIPEDRHGEPFVLIVSCWAGPLDEGERAL
                     QPFHDIAPVVAEHVGPMPYPALNSAFDALVPPGLQHYWKANFVTELSDDAIEAHLVHAP
                     RLPAVNSTVHIYPINGACHRVAQDATAFAYRDATFATVIAGMWPDPADNEANIAWVRDY
                     YEATAPHSEEGGYINFMADDDQGRIRSNYKGNYDRLAAVKRAYDPGNLFHLNQNIKP"
ORIGIN
        1 ctcgccgagg tgctcaccgg tacgatctcc agccggcggc cgctcgcggc gacggcctgc
       61 gccagcgcct cgccgaacgc gttgtacgga ccgccctccg gccccgtcgc aaggcgaagc
      121 cgccgcaccg gaccggagtc ccccgagcat ccggccagcg ccgccgcgac cgcgccgagc
      181 ccggcaccca tcacccgtcg gcggctcacc tctgcgctgg tcacgcccga agcgtagcca
      241 gggatcagac ggccaggggc tccaggtcgc ggtggatccg ccgctcgtcg cgggcgtagc
      301 gggccaggcc gtggtccgcg ccgagcagtc ggctcagctc ggccaccatc tccgcccgca
      361 gtcgggccgc ctcctccttg cggcccatgg agtccaggct gatcgccatg ttggagctgc
      421 tcgccagggt ctcgggatgg tgcgccccga gcacctcgcg cagccgcgtc acggccggcc
      481 gctccagctc cagggcgcct tccggatcgc cctggtcggc ccgtgcgttg gccaggttca
      541 gcgtcgcgaa gaccgtgtgc ggatggtggt cgccgagcac gtcccgcatc agccggatcg
      601 tctgccgcag catcgcctcc gccgtcccgg ccgcacccgc gccccagtgg tagaccgcca
      661 ggttgttgac ggccgccagg gtgtacgggt gccgctcgcc cggcactttc atgtactcgt
      721 ccaccacctc ctgcgccagg accagcgccc cgcccgggtc tccggtcgcg aacaggtccg
      781 aggccaggtt cagttcgcag gagagcaggt ccgggttgtc gaaggtgtac ttggcccggt
      841 agcgggcccg ggtggccgtg gtcagccgga gcgcgtcctc cagccggtcc gcccggcgca
      901 gcgacaccgc caggttcttc gcggccgaca gggtgccggg gaaggcccgc cccagcgtcc
      961 gcttgtaggt ctcgtaggtc cggctgagca gctgcaccga gtcctcatac cggcccacct
     1021 cgcgcaggtc gcgggccagg ttctgcgcgg aggacagggt gtacgggtgc tccggcccga
     1081 gcacctccgt gcgcaggtcg tgcacctcct ggtcgatctc gcgggctctg gtgtactggc
     1141 cgaccagccg caggttcagc gccaggttgt gcgcggcggc cagcgtgcgc gggtgcgcct
     1201 cgtggaagat ctggccgaac ccctcgtgcg cgtcggtcgc cagctccatc gcctccgcgt
     1261 actggccgag cgtgcccagg gtgttggcga gaccgctcat ggtcgcgtat gtgtgcgggt
     1321 gcgaggggcc cagcaccgcg cgctggcgtt ccagggtgcc ctcgtccagc tccttcgcct
     1381 ccacgaaccg tccctgcgag cgcaggatgt tcgacaggtg gaagcgcagg tacaggtact
     1441 gcgggtcgtc gttgcccagc agctccttcc aggcgtcccg cagctccaca cccagcgcgt
     1501 acgcggcctt gaagtcgccg cgcttccaca ggtagcggac gcggtcgatc agcagccggc
     1561 gagtctccgg ctccttgcag taccgggcct cggaggggga caggtgcggc cagatggtgt
     1621 tgaacctggg ccaggtctcc gggttgtcga tcggctcgtc gtcgtccggc cgggcgcccg
     1681 ccaggatccg gtggaccgtg tgccgggcat cccgctgctc ctcctcgctc agctgcgccc
     1741 ggatcatggc ctggaccagg cggtgcacct ggatgctgtt gctcacctgg tcggccttgg
     1801 ccagcgcgaa ccggccgatc tcccggatca gccggccgag caccaccttc tcctggagcg
     1861 aggagtcgta cggcttcagc gcgtcgatca tctccttgcc gtagaggagg ttcgccgaga
     1921 tcggctcggg tccgaagaag gcgcacagct ccagcagtcg tgcggcggcg ggcgagcgga
     1981 accggagccg atccatggac acgttccagg tggcgggcag cggtttcggg tatccggcct
     2041 gccgggccag ctgctccagg tacgcggaca ccggtgtcgc ggtctccgcg atccaggcgc
     2101 ccgcctgctc caccgcgaac ggcagatcgc ccagcgccgc ggccacctga tcggcgtcct
     2161 ccgcgctgag cccgggggcc cggcgctgga ggtgctggat ggactcctcc cgcaggaaca
     2221 agtccatcgg cagcgagtca ccgtactggg accaggtctg gttgcgggag gtcacgagta
     2281 cgtgccccgg tccgcccggc gggaagaacc gcttgagggc ctccggatcg tcggcgttgt
     2341 cgaagaccag cagccaccgc gaggacggaa cccccgccgc agcaggtcga tcgcctcctg
     2401 cgaggcggcc gacatgtcct ccccggtctg cgcgcccagc cgttcggcaa gctccgcgag
     2461 cgcggcgacg atctcgtcgg tctcctccga ggggatccac cacaccaggt cgtaatcggc
     2521 catgaaccgg tgggcgtact ccagcaccac ctgggtcttg cccacaccgc cgagtccgta
     2581 gagggtctgc ggctgcggca gcaccacggc catgccgccg ccgagctggt cgcgcatccg
     2641 ctccagcacg gtgtggcgcc cggtgaaacc ggggttgcgg ggtggtgcgt tccagacctt
     2701 cgggatggct ccggggaagc ggggagcggg ctgggaggag cccgcgacgc ggtccgggag
     2761 cgccatcggc cgatccaccg cgcgcagcag ggcggcggtg gcgtgcacct cgtcgaggcg
     2821 gaacaggtcc accgggtcgt ggtcgatgta cggcgtggac aggcgtaggt cgcccacccg
     2881 cagtggaatc agccggcgtc ggcccccggt cgggtccacg gcggccgcac gctcccacac
     2941 gtccacggcg cgggcagagt tgaggtaggc gccggagagc agcaccaccg tccgggtggc
     3001 ggtgtcgccg ctcatgtccg ccccggcggg cgggtccccg gcggaggcgt ccatgcgtat
     3061 gacccggcag ccggcccggg tgagcaccga ctcggcccag tcagcccaca tccggttctc
     3121 ggggacgtag gagaggaaca ggtcggtggg caagcccggg gggcgacggg tgaaggcgtc
     3181 ccggatccgc aggcgcgtct gctcctccat ggcgggcatg gacgtgacct cgccctcggt
     3241 gatcacggcc gtgagccgtt cgaaggctga aagcagggag tcgctgagcc cggcctcgtc
     3301 gccgaaggtg gccaggatct cctcgtaggc gtaggaggag cggtacggga tctccaccgc
     3361 gcgccagtag gaggtgagtt cgtccccgac gagcccgtgg gggaagcggt cgaacgtggt
     3421 ccgggccagt gcgcggcccg catcggcctt gtccggatcg ctctcgtcga cgcgcatcgg
     3481 gacggggtag atcgtgatgc cccggtcgtt gaagcgctcg tcgatctgcc tggcgacggc
     3541 agcggcgccg tcgatggact ggtcggacag ggtgaaacag tccaccagga ggtccgggag
     3601 gtggacggtg cagatgtccg cggtgtcgga caggccggtg cggctgtcga tcaggacgta
     3661 gtcgtagttc ctcttcatgt cggcgcgtaa ggcgtcgaag aagagaccgc cgccgagccg
     3721 gtcgtagaag ttgtcccagt cgaaggtgga gaccgttgcg gagtactcgc ggttctgccg
     3781 gccggccgag acgaagtcga gggcgccgcc gtccgggaat tcccagccca gggcctcggg
     3841 ggtgagcgat acggcgtgcg gctgtatccg cgcgtagtcc tcgtgccatg cgtcggcgcg
     3901 ctgcaccggg ctggtcgcgg cccacgcgta ctcgctgatc agttcgatga cgccggtggt
     3961 ggcccccagc gtggagggat ccaggaaggg gtggaagaag cggtgcaggc cgggagcctc
     4021 caggtcccag tccaccgcga ggacccgttt gccgttggct gcgaggatcc aggcggtgtt
     4081 ggccagcgcc atggtgcggc cggtgccgcc cttgtacgag tagaaggtga cgatgcgtcc
     4141 gtcacggctg tcactgtcac gactcactgt catccgcgtc cccccggccg gtgtacacat
     4201 cctccattga acgggtccga gccctgcttg tgccagagcc cgggccgtcc ggggctatcc
     4261 gccccccgtc tccacgccgt cctcgtccct cggcggccat ccggacgtcg ccttccccgg
     4321 cgccagccgc tccaccacct cggccaggtc ctggcaggcc tgctcgatcc tcgcgcggat
     4381 gatgttctgc tcggtgacga tcgccgacag cagcagcgag gtcaacgccg cggagccgtt
     4441 gagcgcctgg aggttgacca tggcttcgac cagtgtgtgg tggtcgaagg ggccttcctg
     4501 ggcgatcgcc gccgagttcg ccatggcgga cacgagcagc atgcacggcg cgctgccctc
     4561 cagctggaag cgcagggccg cccagatgag gagcgggaag acgaggaaca ggagcgccat
     4621 gtcgctcctg gtcgccagca gggtgacggc gaaggccgtg accagcaggg ccgccgcctc
     4681 cgcccaccgg taggcgccag agggaaggcg ggccatgcgc aggaccagga gcagcggcgc
     4741 gatcacgagc acgcccatcg cgtcgcccgc ccaccacgtc caccacacga tccagaatcc
     4801 gctgtcgggc aggctgtcgg tgaccacgag cagccaggca ccgatggtgg cactgatcag
     4861 catcggaacc aggccgccca ggaagaccag cgccacaccg tcccggagcc ggtccagtgt
     4921 cacgcggaag ccgacccgtc gcagcatcag gtaggcacac acgggagcga gggtgttgcc
     4981 cgcgacgatc ccgaagccga cggggtcgat cgagccgctg atcgactgga tgacgagaca
     5041 ggcaccgagt gcgatacccg gccagacgcg gagccccatc cagagcaggc agacgagggc
     5101 gatgcccgtg ggcggccaca ggggtgtgac gaccgcgccc tggatcacca cctgctggag
     5161 caggccgatg cgtccgccgg cgaagtaggc ggcggcgacg atcaggttca ggagcaccgt
     5221 catgcacaga cgccggaagc cctcggtgcg catcacttca tcagacaaca gcccgcgccc
     5281 gccgacggag catgacacgc gctacggtgc cctgcgcatc atccggttcc gccccggacg
     5341 gcctcatggc gcagcacgag cacggcggcg tcgtcctcgt gaccggtgga gtcggcgacc
     5401 ctcatcacct cggcggccag ctcaccgggg tccgcgcccg ccttcgcccg gaccacctcg
     5461 gcgacgcgcg ccagaccccg gtcgatgggg aaggacggtc cttcgacgac gccgtcggtg
     5521 accaggacga acgagccccc ggtggccagc cggcggcggg tcacggggta ctgcgagccc
     5581 ggcagcatgc ccaggggcag accgccttcg tcctccacga tgccgtgctc gccgtcggtc
     5641 gtggcccaga ccccgggaac gtggccggcg cgagcggtct ggatctccca cgtgatgggg
     5701 tcgaagcgga gaaaggtgca ggtcgcgaag agatcacagt ccaccgagag cagcagatcg
     5761 ttggcccggc tgagcacctc gcccgggtcg gcggcggacg cggcgacggc gcgcacggcg
     5821 atgcggacct gccccatgaa cgcggccgcc tcgacgtcgt ggccctccac gtccccgatg
     5881 gagaagccca gagcgccctc ggggagcagg aagccgtcgt accagtcccc gcctatgctc
     5941 aggccgtgac gggcgggtgc gtaccgggcc gcagtgcgca ggcccggcac gccgggaagg
     6001 gacgccggca ggatttcccg ctgcagtgcc tcggccagct cgacccgggc ctgctggagc
     6061 tccgcccctt cccgggcctg ggccgtgagc cgcccgagcc tggtcagcag gtcgtccgcg
     6121 gtcgccgacc gcgtggaccg atggtggctc atggaccgct ccgacatacg tccgcttcag
     6181 ttccgacaca gatggccaga atcatcatat ttcggatatt cgctgatcac atcacgggcg
     6241 caggctcctc aggggtagct caccaggttg gccacgttgg tggacgcgtt cgaagggccg
     6301 cccctgtcgt tgatgacgtg ccggatggtg ccggtgccgc cgagcgagac ggtcaccatg
     6361 ctctggaagc gcacgttcgg gttgccgggt gcctcgaagg cgcgttcggc ggtcacgctg
     6421 ctgttgacgt tgaagaagca gtagctgccg agcccgaagg cctggtggct ggtcacgtgg
     6481 tcggccacct tgtacgccgc gtagccctgg gtggagccgt tcatccaggc ggcctggttc
     6541 ggcgggtcgt acggcatctc gttctggtag aagtacgtcc gcccgccgtt gccgttccac
     6601 acggtctggt gcttctggta gtgctcgacg aacagtccgt acatggtcac gccgtcgccg
     6661 ttgaccacga gtcccgtgtc cgccgtgttg ctgttccagc cgatgccgct gccgtggtca
     6721 ccgcgccaga tccacatgtg gtcgccgatg acgtggtcgc tgttgacgac gaggctggtc
     6781 gtcgccttgc ccacggcggc tccgccgacc cggaagaaga cgtcgtgcag ggaggtgggg
     6841 ttcgccgagt ggttggctgc ggcgccggcc gggccgacct ccatcagggt ccgcgaggtg
     6901 gtggtgcccg cgtcgaagag cacgcccgcg atcttcaccc cgtcgacgtc ggcgaccgtc
     6961 atggccgtga ccccgttgtc ggggatgaac gtggcgaggc cgagtccgag gacgacggtg
     7021 tcgggccggg tcacccgcag ggtccggtcg aggtggtaga caccgggcgt gaccagcagg
     7081 ttcttgcctt cggccagggc cgcgtcgatc tgtgcggcac tcgctccggg cttgacgaca
     7141 tagaactggt ccatgccgag cgaactgccc gcggcgttcc cgttcgccca gctggtggcc
     7201 gaggaatcgg tccgcaggga cggcacgaac accttgtagg cgcccgtccc gtccacgtac
     7261 aggaagggct tctcgcggac ggtcggcgtc tggttcaccg tggtgtacgg cgggttcggg
     7321 aagctggtgg cggggacgcc ctggctgccg acgaagacca tgttccagtt ggacccggtc
     7381 cagccgccga gctgcgagtt gcgggtcagc cactgctgct gcgtccccga ccgcacctgg
     7441 ccgtcgatct tgctgtccgc catgaatccg ccgctggccc agccgccgtc gtccagggcc
     7501 aggttgccgc gcacgtgcat gcggcggtac ggcgcggcct gggacaccgc ccagcggtcg
     7561 gtgccgcccg tcgggttcac cgacaggttc tcggcgccgc gccagaagtt ctgggtggcg
     7621 ttgtgcggcg ggaaccagtc cgcctccacg tgcacggcgc cgttgatcgt gaccgagtcc
     7681 ggtgactggc cgagaccgag gacctgggtg tagaagccga cgttgacgtc gttggcgtac
     7741 gtacccggct tgaacatcac cgcgtagcgc tgggcgccga actggttggt ctcctgctgc
     7801 cggaagatcg agtcgagccg gctctggatc gtggacgagg gcatggaggg gtcgaagacg
     7861 accacgttcg ggccgaggtc gacgtccccg ggggcggtgt tcacgggagc cacctggaag
     7921 cgttgggccg ccgtgccgtt gcaggtgtac tgcaccagct ggacgctgtc ggcggtcgag
     7981 gcggacggca cgtcgaggca cttgccgctg tggcggctga cgaagtggta ggcgccaccg
     8041 ccctcgtcga cggccctcca ctgctggttg gctccgccgc cgtacgtcca caggtgcacg
     8101 gccgcgttgt ccgccgagga cgtgtcgctg acgtccacga cctgagcggc gtcgttgcgg
     8161 ttgccgatcc ggacgaagcc gtccgtggtc ggggtgaaac tccactgctg cgccgaggag
     8221 ttgttgcagg tgtactgctg caccgcggtg ccgttcgcgg tcgcggcgga ccgcgcgtcc
     8281 aggcatttgc cgctgccggc gttgaccacc gtggaccagc ccgtgggcag ggcggcggcc
     8341 gcctgcgccg aggcctgcgg ggtggctgcc tgagtgggct ggacgacggt gagcaccgag
     8401 acggccgcgg ccacgaaggc cgtgagccgg ctcccggtgg tgcgggtcat cgggtcaccg
     8461 tccacttctg gttggcggct cccgtgcagg tccagatctg cagccgggtg ccgttggccg
     8521 agctgttgcc cgcgacgtcg aggcacttgt tcgcctgcgg gttgacgaca tctcgggccg
     8581 cggggacggc ccactgctgt gcgggggtcc cgttgcagtc gtagagctgg accggcgtac
     8641 cgtccgcggt gcctcccgag gccacgtcca ggcacttgcc gagcgcgcgg atcgtgccgt
     8701 cggatccgac ggtccactgc tgcgcggcgg tgccgttgca gtcgtagagc tgtacgggtg
     8761 tgccgttcgc gctgttggcg ccggccacgt cgacgcactt gccgccgagg ccggtgatcg
     8821 gacctccgcc gcccggctgg ccgccgccgg aggtgacccg gacgtagtcg accaggagct
     8881 gcccggggaa gaccgtgctg ccgtcggggt ctcccggcca gtagccgccg accgcgaggt
     8941 tgaggatcag gaagaagggc ttgtcgaaga cccactggcg gccgccgagg tctgcggggg
     9001 tgcgccgctg gtagacggcg ccgtctactg accaggtgat cgcgttcggg ctccagtcga
     9061 cggtgaaggt gtggaaggcg tcgacgaacg cctggccgcc gggcagggtg tacccggcgc
     9121 cgatgccgcc ggagccggag taaccggggc cgtggagggt gccgtggacc gtggacggct
     9181 cgaagcccac gttctccatg acgtcgatct caccagaggc gggccagccg acctggccga
     9241 tgtcgttgcc cagcatccag aaggccggcc acatgccctg cccgcgcgga accttcatcc
     9301 gggcctcgac gcgaccgtac gtggtggtga acctgccggc cgtgttgagc cgggcggagg
     9361 tgtactcgca ccttccgtac cagcactggt agttggccgg gttctcccgg cgggcggtga
     9421 tgaccaggtt gccctggccg tcgagggccg cgttgcgatt gcccgaagtg tagaactgcc
     9481 gctcgtggtt gttgacgttg tcgcccgtct cgatctgcca tttcgcgccg tcgacggccg
     9541 agcccgcagg cccgttgaac tcgtcggaga acgtgacggc cgcggccgac gcggcgcctt
     9601 cggcggcaac ggcggccggt gcgggggcgt gcagcggcgg cacgccctgt gccaggccgg
     9661 tggacagcga cgcgagggcg aggagcgaga cggcggacag cagcaaacgc cgcgataggt
     9721 gcgggaagtt catggctctc ccttccggga gtgtggggga aggccggaca ggatcgtcat
     9781 gaccatgcca tgcccttagt tcactacgtg atttaagaag tgaacaaatc ccctgtcaag
     9841 agcttggcct ggaccatata actggcttgt ccagcatgtg agttaccgca tgcaagacat
     9901 ggaacggacc gtcacgctca ggtaccttcc atcacgtcgt cgccacaccc ggggcacagg
     9961 gccccacggg actcgcggcg accaaaggaa ccgacgtctg ttgacgacag ccacagaagt
    10021 cttcacggtc cagcctttca ccccgcactg ccaggtgatc cacaccgaag gcgaccacgc
    10081 ggtgataggc atctcaccgg gcaacagtta tttctccgcg cagcgcgtca ccgacctcgc
    10141 gcactggggt ctgcgcaact tccggcaggt cgacctcatc tacacggaca tgcacgtggc
    10201 cgagatgtac gaggccctcg gctatcccgc ggacgaggcg cggcgcaagg cggtcaagaa
    10261 cctgcgcggg gtccgcgcga aggtcaacaa cgccgccgcc gaggccgatc ccacgggcac
    10321 ccgcctacgc gcccgcccca tgtcgtccct caccgacatc ccggcctacc gcaggctcca
    10381 cacccacctg aacaccctgc tcggcaccga cccggagttc cgcgagacct gcaacgccct
    10441 ggtcgacgcg ttcctctcct ccaaggtcct cggcgggaag accgccacgg tccggcagcg
    10501 cgaggtctgt ctcgagtacg tctgcgccga ggccccgctc ttcctcgaca ccccggccat
    10561 tctcgacgtg ccctcgtccc tcaactgcta ccaccaactc ctgccgatgg cagagctgtt
    10621 gtattcgcgc ggttcgggcc tgcgtgcctc gcgcaaccag ggacacgccg tcatcacccc
    10681 cgccgaagga gaatccgatg cccactgaca cacttctcga ctttccgttc tccgcgcgcg
    10741 gcgaccagct cccccacgag gtcgagcagc tccggaccga accggtcaag cgggtccgta
    10801 cgatcgccgg ggacgaagcc tggctcgtct cctcctacga cctgtgcaag caggtcctcg
    10861 aggatcccag attcagcctc aaggacacct cggctcccgg cgtaccacgc cagtacgccc
    10921 tgacgatccc gcccgaggtc gtcaacaaca tgggcaacat caccggcgcc ggactccgca
    10981 aggccgtcct caaggccatc aaccccaaga ccgacggcct gaccgactgg atgcgcgcgc
    11041 aggccaccac cctcgtggac agcctgctca ccgagggcgc gccggtcgac ctgcgcaccc
    11101 agttcaccag tccgtacgcc gagcagctcc actgccgcat cctgggcata cccggggaag
    11161 acgccccgcg cctggcggcg agcctcgaca tcgccttcat gaactcggcg tgccccatca
    11221 ccggagccaa gctcaactgg gaccgggaca tggcctacat ggtcgagcgc ctcgacgacc
    11281 ccaccgtcac cggcctcatg gccgagctgg cggccctgcg caaggacccc gactacgccc
    11341 acctgacgga cgagatgctc gccaccgtcg gagtcaccct cttcggcgcc ggcgtcatct
    11401 ccaccatggg cttcctcacc atggccctgg tctcgctcat ccagcacccc gaggtgtggc
    11461 agcagatccg ccaggacccc tccaagatcc ccgcggcggt cgacgagctc ctgcgcatca
    11521 acctctccat cggagacggc ctgccgcgcc tcgccctcga ggacgtcacc ctcggagacg
    11581 tcgaggtgaa gaagggcgaa ctcctcctcg ttctggtcga agccgccaac accgacccgg
    11641 acgtgtaccc cgacccgcac accgtggaca tcgaccggcc caacgcgggc acccacctct
    11701 ccttcggcgg cggccagcat tactgccccg ccaccgccct cggcaagagg cacacggaga
    11761 tcgccctcga ggtactcgtc gagaagatgc ccggtctcca gctggccgtg ccggtcgagc
    11821 aactcgtctg gcgcacccgc ttcatgaagc gcctgccgga gcggctgccc gtcctctggt
    11881 agcagtccga cggccgcggc gcgggcatcc gaggtcaccc ttcgacctcg gatgcccgcc
    11941 gcctgtggcg cgtcaccccg gccgtcccgg ccgccgcagc aggtagacgg cggcggtcga
    12001 gacgaggacg gccatgcagg cgatgaacac caggcccgcg ccctccaggc ccatcgggcc
    12061 ggccaggaga cccacgccga tcaccgggac cgagatgccc gcgtacgcga ccacgaacag
    12121 gctcgagatc accgccgcgc gctggtgcac gggagacgcc tcggtcaccg ccgacatcgc
    12181 cccgcggaac gcgagcccct gtccggctcc gccgacgagc gcgctcagga ccaccagcga
    12241 cagcaggtcc cagtacagcg cacccgccag cagcgccagc ccggcgagga gcccggcgca
    12301 gcccaggggc agcgagcgcg tcaccccgac ccggccgacc agcagctgcc cggcggtcga
    12361 ggcgaagaag gccagcgcga cgaccagccc gctcacggcg tggttgtgca cgtccaggga
    12421 ctcggcgagg aacgccgggc tgaccgaggt gaacaccccg aacagggcga accccacgaa
    12481 cgaggcgatc gccgcgggca cgaacaccgc ccggacttcc gcgggcaggc cgggccgctg
    12541 tggccgtacg gtgctcaggg gccgccgctc ccgtaccgtc tccgcgagcc gcagcaggac
    12601 ggcgaccgag acggccacca gggcgaggtg cacggcgaac ggcaggtaca gcggcgcgac
    12661 ggcgtactgc gcgagcactc cggcgagcag cggaccgcag cccagcccgc ccatgttggc
    12721 ggccgtcgcc accagcgtgg ccttggcggc ccggccggac ggggccagct ccatcacgta
    12781 cgcggtggcg gcgccggtga acaggccggc ggacagtccc gagaacagcc gccccgcgta
    12841 gagccagccc atcccggtgg cgcacaggaa gcagacggcg ctcgccgccg cgcatcccag
    12901 gcccgcgagc agcaccggct gccggcccac ggcgtccgag gcgttgccca ccagcagcag
    12961 cacgccgatg accccgaagg cgtacacggc gtacacgacg gtcaccatca gctcggagaa
    13021 cccgaacttc tcctggtaga ggccgtagag cggggtcggc agcgtggtgc ccgccatgca
    13081 cacggcgaac accgccgcgc cgagcaagca ctggcgccac ccttggcgat caccgtccat
    13141 gccgccgacg gtaaccccgc accccgtccc cgcccggcgc ggcgtgcccg gcgcgccgcc
    13201 ggggtacggg accgcgggtc aggccgacag caagatcggg ttggtcagcg ccgccggcgc
    13261 accggggagc ggcggggccg aggcgggatg gcgggcctcg gcgcggacgt aggaggtacg
    13321 ggacggggtg gtgcgccatt cgaggctcgt ggccgcgggg gcggtgaacg ccacgccctg
    13381 gtcggtgacc agccgcaggt cgtagctgcc gctgccggtc acggtcagcc ggatcaggac
    13441 ctcctcggtg tccgccgtgg cccggatccg gtcgccgatg cccgcgtggg cgccccgtgc
    13501 ggtcaccgcg tcgaaggtca ccgacacggc cgaggactcg gccgcgtagc tgtggccggc
    13561 gcgcagaccg gcgagtatcg cctcgcggga caggtcctcg gccagcacca ccgtctgcgg
    13621 gctgccgacc cggtcggggt cgcggtggta gtcgctgctc gcgaggaccg ggagccactg
    13681 cccgcgaccg gcggtgaggg tggcgtccca ggatgcgagg gccacttcgt catcgggggt
    13741 ccaggggccg ttccacacct ccaccgcgtc cgcctcgccg aagccgaact tccaggcgca
    13801 gcctatgcag gtggcgtgcg ggtgggcggg gacgaccagt ccgccggcct tgcgtatgtg
    13861 gtgtgcgaaa cggtcgaagc gctggtcgcg ggcccggtag cgccagtccg tgaaggtgcc
    13921 gggtgcggtg ccgacggcga cgacgtggcc ggtacgggtg gtgatctcct ccccggtaag
    13981 gacgagcagg ccgggatccg cggcggcgac cgggccccag gccgcgtgcg ccgcgtgggt
    14041 gttgtgctcg gaggagttga tgaagtccag acccgccgcg cgggccagcg cggcgagttc
    14101 ggccggggtg cggcggccgt cggagtgcac cgagtgcagg tggcagtcgc cccggtacca
    14161 ggcccggccc cggccacggg cccgctgcgg cgggtagccg gggacggcgg gcgtgcccgg
    14221 gtcgccgtag gtgaaggtgg cggtgatctc gtaggtgagg ccctcgggtg ccatcgtgta
    14281 cggcccgagc gccacgtgcc agcggccggg ctcgaggggt ccgggcagat agccgggggt
    14341 ggcggcgtcg gcccgcagga agaactcgct gcgggcgccg cccgaccagc cgcggaagcc
    14401 gtggccgccg agccccgtgc ctcgctggtc gaagatgccg atgtcgagtg cgttgcccgg
    14461 ggtgccgggt ggcaccgggg gtttcgcgta ggtgtaggag acgcgcagct ccgcggtccc
    14521 cggcgggacg tcgaggggga ggtacaccca gtccggggcg ccgggcgcga tcgtgccgcg
    14581 cagggtaagc gtggccgtgt cccggccggt gggggtgagc ggcccgacgg ccgccgtcac
    14641 cccggatgcc agtagttctc gtctactgag agccatacgg caacggtggc agccgcgggt
    14701 gaacccggag tgcacggcgc acgacgcgga gggacagcgc ttccgcggcg agcccgtcgg
    14761 gtgcgggtca ggagtcgccc cggaggcggt cgcgctggat ccggtgggcg agggcccggc
    14821 gcaccgatgc cgatgcggtg ccggtggcgg cggcggtggc ggccgccttg gcggggacgt
    14881 cgagagcgcg gccgatctgg tcgtacgcgg cctggtggcg tgcggtcagc tgcccccggt
    14941 gcaggcgcag gtcctcttcg acgagacgtc gcagttcgtc gacgtcccgg ggcgtgaacc
    15001 gcttccggcc ccgggcgagg gcgtcgatgg tgcgggtaca gcgcgcggcg ccgtccagcg
    15061 tctcggagat ttcctcggcg aggccccaga gacgtccttc gagccacggg gcgtcgtact
    15121 ggtcgaggga gagctccaac gggggcagcg gatcgcccgt cttgtacttc gtgacctgct
    15181 tggacacggc gggctggctc atgtcggtaa gccgggcgat cttgtcctgg gtccagccga
    15241 agccgacgaa caccttgatc atttcggtgc gcagcttccg catctcctcg cccgcgcgga
    15301 tgacctcgtt catcccggcg agcatccgct cggcctgttc ctcggtcaac gtctccgggt
    15361 ttcggggtac acccttttcg cctgccacaa ctcggttata cacccgagag ccggatcgcc
    15421 ataacccagt tgtacaacct ggttatggat ggcaggatcc gcggcatgca caccttctcc
    15481 gcagctgacg gaacccgcct cgcctaccgc acccacggag acggcgaccc cgtggtctgc
    15541 ctccccggcg gccccgcgga ctccgcctac ctcggcgacc tcggcggcct gtccgcgcac
    15601 cgccggctga tcgtcccgga cctgcgcggc accggccggt ccgcggttcc cgaggacacc
    15661 tcctcgtacc gctgcgaccg cctcgtcgac gacgtcgagg cgttgcgcgc acacctggga
    15721 ctccccgcga tggacctgct cgcccactcc gccggggcga acctcgcggc gcagtacgcg
    15781 gcccggttcc cgcaccgcgt gagcagactc gcactgatca cccccggtac ccgggccgtc
    15841 ggcatcgaga tcaccgggga gacgcggcgc gagctcgccc aactccgggg aaacgagccg
    15901 tggttcccgg ctgccttcgc cgccctggag gcgatcaccg agggcacggg cagcgactgg
    15961 gatgccatcg cccccttctt ctgcggccgt tgggacgatc gcgcgcggca gcaccaggcg
    16021 gccggccggc cgcgcaacat ggaggccgtc accgtcttcg cggccgaggg agccttcgac
    16081 ccggagacca cgcgcgccgc actcgccgcc ttcgcggccc ccgtcctgct gctcgccggg
    16141 gagttcgacc tgaacagccc gcctcggtcg acggccgagt tcgccgggct cttccccgac
    16201 gcctcgttcg tcgtacagcc gggggccggc cactacccct gggtggacga cgccgaccgc
    16261 ttcgcggcga ccacggccgc gttcctgggg tagttccccc gcccgaggcc ggagccaggc
    16321 gatcgcggga aatctgccag actccccgca tgccgccttt cacgaccgtc gccgcctacg
    16381 ccccgcccgg tgtcggcatg ctcgccgtcg gtgtcgtcac cgaggtgttc ggcccgcgtg
    16441 cggggctgcc ccgcttcgac ttcgcgctgt gcgccgaccg gcccgggccc gttcccaccg
    16501 acctcggcgt accgctcgcg gtcgagtacg ggctggagcg gatggccgtc gccgatctcg
    16561 tggtcgccct gccctgggcc ggcttccgga caccgctcgc accggccgtg ctggaggccc
    16621 tgtccgccgc tcacgaacgc ggcgccctgg tcgcggccca ctgcgtcggt acgtacgccc
    16681 tcgccgccgc cggactgctc gacgggcggc gggccacgac gcactggcgg ttcgcggagc
    16741 tgctggcccg ccgccatccg gaggtcacgg tcgatcccca tgccctctac gtcgacgaag
    16801 ggcgcgtcgt cacgggggcg ggagcggcgg cgggtttcga tctctgcctg cacctggtca
    16861 ggcgggagca cggtgccgcg gccgccaacg ccatcgcccg cgacctcgtg ctgccctccc
    16921 accgcgaggg cgggcaggcc cagtacctcg ccgcccccgt ccccgaggac gctcaggaca
    16981 cccgcctcgc cgaagtcctc gcctgggcgc gcgagcacct ccacgagcag cttcccgtcg
    17041 cggaactggc ccggcgtgcc gtgatgagca agcgctcctt cgcccgccgc ttcaccacgg
    17101 cgacggggac caccccgcac gcctggctgg tcagcctccg gctgagccgc gccgaggagc
    17161 tgctggagac caccgacctg tccgtcgagg agatcgcccg cgcggtgggg ttcgcgagcg
    17221 cggccgtgct ccgcgagcag ttcgtacgcc ggcgcggtgt gccgccccgc tcctaccggc
    17281 gctccttcac ccgcaccccg tagcgcccgt tcatggaaat ccgggaacgg agcaccacgt
    17341 acgggcaggt cacccgggcg gtcgcatcgg agccgagcct cgcgagcggg gacgcccgac
    17401 ggcgctaggg tgagccgaac ccggccgtag tgatcgcgag caaggtgggc agctgtgtcg
    17461 acgtcgagtc aagagatgtt cggcgcgccg tgctgggtca gcctgatgac cggggacctc
    17521 gactcggcgc agcagttcta cggggccgtc ctgggctggc acttccgccg caccaggctc
    17581 ggtgacggct tctccatcgc cttccgcgac ggcgcccccg tggccgggat cggcgctctg
    17641 gcccagcggc tggcccttcc ggccgcgtgg accccgtact tcgccgtcga cgacgccgat
    17701 gtcacggcgg cgcggatcgt cgagcgcggc gcgaccatgg cggtgggccc ctcgccttcg
    17761 gcaccggacg cgccgcgctg gcctccgacc ccgacggagc ggtcttcggg ttctggcagg
    17821 gccaggtcat ccccgactgg acggtcggcc agggcagcgc gcccgcctgg ctggaactgc
    17881 gcacccgtga cgccttcgcc gcggccgtgt tctacggtga ggtcctcgag tgggccggcg
    17941 aacggcccgg cggctgcacg gtctcctacg aacacgacca cgtcgtgctg cgccacggga
    18001 acgacaccct ggcgcgcatc gtgggcgggg ccctcgagga agcccccgat ccgcaggtcc
    18061 gaccgcgctg gcacgtccat ttccgcgtcc ccgacctcga ggcgacggtg gaaatggcca
    18121 ccggtctcgg cggtcgcgtc gtctcgcccg tggacaccac gagcaccagc cgctcggtcg
    18181 ccctggccga tcccgacggc gcgttgttca ccgtggtggc cccgcagaac cggtgacggg
    18241 acttcggcca ccgcagctct ggagggctga catgaggaag atcatcgcag ggctgttcat
    18301 ttcgctcgac ggcgtcgtcg aggcacccga ccagtggcat ttcccctact tcaacgacga
    18361 gatgggggcg gccgtcgacg ccaccctcgg ctcggtcgac accatgctgt tcggccgcaa
    18421 gacgtacgac agcttcgcgg gggcctggcc cgagcgggag gccgcgggcg gggaggacgc
    18481 cgccttcgcc aagatcctcg gcgacgcccg caagatcgtc gcgtcgaacc ggcccctcga
    18541 cttctcctgg cggaactccg agcggctcga gggcgatctc gtcgaggcgg tgaccgcgct
    18601 caaggccgaa ccgggcccgg ggcccatcgg aatgagcggc tcggtctccg tcgtgcgtca
    18661 gctgctggcg gcgggactgc tggacgagct gcacctgctg ctgcacccga tcgccgtccg
    18721 caagggcatg cggctgttcg acgagggcga ggctccggtc ccgctgacgc tgatctcctc
    18781 ggagaccttc cggacgggcg tgctgaacct ccactacgcc cccgcggagc gggtcggcca
    18841 gggaacgtac gaggacgcca agacccacct gcggcggcag cccgaaggct gagcggtgtc
    18901 gggttgaggc ttcgtggcac cggcgtagcg tggaatgtgc tcgagagtca gcggcacgac
    18961 gcacgcgagg agcaatcgcc atgggcaagc cctcgatcga tcagttgcgg gaacgggcac
    19021 gcggggcggt cgtcaccccg caggacgacg gttacgacga ggcgcgcgcg gtcaacaacg
    19081 ccatgatcga caggaggccg gcggccgtcg tgcgctgcgc caacgcgggc gatgtgatgg
    19141 ccacggtcga cttcgcccgc gagaacgggc tcgacctggc ggtccgcggc ggcgggcaca
    19201 gcgtgccggg cttcggtacc tgcgacgacg gcgtcgtggc cgatctctcc gcgatgcgtt
    19261 ccgtccgtgt cgatcccgtc cgccggacgg cccgtgcgga gggcggagcg acctggggcg
    19321 acttcaacgc ggcgacgcac gccttcggtc tggccgcgac cggcgggatc atctccacca
    19381 ccggtgtcgg cggtctcacc ctcggcggcg gcatcggcta cctcgcccgc ggactcggcc
    19441 tgagctgcga caacctgctg tcggccgatg tggtgaccgc ggacggccgg ctgctcatcg
    19501 cgagcgagga ggagaacccg gacctgttct gggcccttcg cggcgggggc ggcaacttcg
    19561 gcgcggtgac ctccttcgag ttccggctca gccccgtgaa ggacatctac ggcgggccga
    19621 tcctgtacga actggaggac gcgcgcaccg tcctgcgcgc cttccgcgaa ctgatcgccg
    19681 acgcgccgga gcagctcggc ggcttccctg ccttccagat cgcaccgccg ctccccttca
    19741 tccccgagga ccggcacggc gagcccttcg tcctgatcgt gtcgtgctgg gccggaccgc
    19801 tcgacgaggg agagcgcgcc ctccagccct tccacgacat cgcaccggtg gtcgccgaac
    19861 acgtcggccc gatgccgtac cccgcgctca acagcgcctt cgacgcgctc gtaccgccgg
    19921 gcctccagca ctactggaag gccaacttcg tgaccgagct cagcgacgac gcgatcgaag
    19981 cgcatctggt gcacgcgccg cggctgcccg ccgtgaactc gaccgtgcac atctatccca
    20041 tcaacggcgc ctgccaccgc gtcgcacagg acgcgacggc cttcgcctac cgcgatgcca
    20101 cgttcgccac cgtcatcgcc gggatgtggc ccgaccccgc cgacaacgaa gccaacatcg
    20161 cgtgggtccg ggactactac gaggcgaccg ccccgcactc cgaggagggc ggctacatca
    20221 acttcatggc cgacgacgac cagggccgga tcaggtccaa ctacaagggc aactacgacc
    20281 ggttggcggc agtcaagcgg gcgtacgacc cgggcaacct gttccacctc aaccagaaca
    20341 tcaagccctg atccgcctca gcgctccgac agttccgacg gggcctcctg gaccacccag
    20401 ccgttgccgt ccgggtcctc gaacgtcatg aacgagttcc aggtcccgcc cttgccgtcc
    20461 tcccagcccg tggcgccgac atggcggatc ggggagacgt ccacaccccg gtccaccagc
    20521 tcccctcggg ccgcctcgat gtccgtgacg cagatctgga gcccctggag cgtcccgggt
    20581 gccgtcacct tcgtaccggg cgccgcgggc atcccgtgga gcatcgcgat cgaacaccgg
    20641 gagccgggcg gggtcatctg gatgatgcgc acaccggggg ccacctcgtc gtccaggtcg
    20701 acgttgaa
//
