LOCUS       NZ_CP102509            20849 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 756752
            Orig. end                         :: 777601
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="3"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20849
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10849](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     misc_feature    complement(762..775)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     misc_feature    772..788
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
     gene            complement(890..1303)
                     /locus_tag="JIW86_RS04155"
                     /old_locus_tag="JIW86_04155"
     CDS             complement(890..1303)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126290.1"
                     /locus_tag="JIW86_RS04155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04155"
                     /product="hypothetical protein"
                     /protein_id="WP_257552552.1"
                     /transl_table=11
                     /translation="MVTDAEWARIRRGLRFGQVFEGTVAQVPRPGAIGIFVDIGLSVGG
                     FVDALWLPDESEDWPAEGTVADFEIWWADSRQQIRLMPSDSRYLRNDFTALVERFRPNW
                     SSDIGQPVRDPGPATPDELRTALRSDGPPTNSP"
     misc_feature    complement(1303..1316)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    complement(1542..1553)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.84"
                     /tool="antismash"
     misc_feature    1711..1726
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.03"
                     /tool="antismash"
     gene            1895..2974
                     /locus_tag="JIW86_RS04160"
                     /old_locus_tag="JIW86_04160"
     CDS             1895..2974
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04160"
                     /product="hypothetical protein"
                     /protein_id="WP_257552553.1"
                     /transl_table=11
                     /translation="MALIPATLTTASASEAPTPAVPGVTFGKQTTDPQKVRDFWTPERI
                     ARALANEKTDQAGIARDAAVAKAAGEAPEDAARSTAPRLTAEAPQILPAKKASVSTMGA
                     AADVPEMPVAQKVPFPQNAPATVVGKIVFIDDAGKEHGCSGASIAADGNNTVWTAGHCV
                     HPGDGRGSDGFYDLVLFIPGYKESLDTPGDYDAPWGEWVAQSFVAPQAWTRDKDYDEGD
                     LAAFTVKAPTGYTNLSDTVGALGYKFGYGSDWSDIIDSGFPGEGYNRTDMDGYTQFYCT
                     GDVVDAVDWYPWDNRLEMNCDMGAGASGGPMATTEGQIVGANSHVDTDDQDQRINDHLY
                     SSNHGDQAVAVINAINDAN"
     gene            complement(3023..3352)
                     /locus_tag="JIW86_RS04165"
                     /old_locus_tag="JIW86_04165"
     CDS             complement(3023..3352)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04165"
                     /product="hypothetical protein"
                     /protein_id="WP_215148602.1"
                     /transl_table=11
                     /translation="MTRRQVWAIAAATFVAGGALGFAVAAVQEDELPPVTVGMPQPAES
                     KVPETRAGLVKAWTAELEHAGKELPDGWEVLTTDEIRGRYLHQRILNAPTAEDIDPDMG
                     RRVDG"
     gene            complement(3612..4016)
                     /locus_tag="JIW86_RS04170"
                     /old_locus_tag="JIW86_04170"
     CDS             complement(3612..4016)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016639900.1"
                     /locus_tag="JIW86_RS04170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04170"
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="WP_257552554.1"
                     /transl_table=11
                     /translation="MTTESTSHAADIEAISRLVATVERSQQNKDPEEFLGLFHPDAIWT
                     TAHGKVLIGLDAISEFTRKVLRAATWDGKVTYEVIHVLFIRPDVAAVKVRQRYLAPGEE
                     SEGAPLYVISKQADGRWLLTACQNTGVVAD"
     gene            complement(4028..4267)
                     /locus_tag="JIW86_RS04175"
                     /old_locus_tag="JIW86_04175"
     CDS             complement(4028..4267)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04175"
                     /product="hypothetical protein"
                     /protein_id="WP_257552555.1"
                     /transl_table=11
                     /translation="MLLAAKIVPDMTVVRDYDRVEFRGTGPGVPADVQSRVFDPCVDSQ
                     VVHDRPGVTVPGPVEEGISHRHVITRLTSISLEV"
     gene            complement(4623..5147)
                     /locus_tag="JIW86_RS04180"
                     /old_locus_tag="JIW86_04180"
     CDS             complement(4623..5147)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676093.1"
                     /locus_tag="JIW86_RS04180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04180"
                     /product="hypothetical protein"
                     /protein_id="WP_257552556.1"
                     /transl_table=11
                     /translation="MKATRSLFNRITSVAVTILLALSATACSDDGTPKARTGGPVWVEG
                     QGAPEAAGFMRVQTPTGATEVKGAVQRGFQDTIYLISFTASAAEAETFIADLRPQEALR
                     ANTPRDPKAYTTITPWSHLGLPEPETEQKVRTASVCPPCIKDERRSTVQSITIFIQDTG
                     DGRVRVYLKAF"
     gene            complement(5144..6553)
                     /locus_tag="JIW86_RS04185"
                     /old_locus_tag="JIW86_04185"
     CDS             complement(5144..6553)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239012.1"
                     /locus_tag="JIW86_RS04185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04185"
                     /product="hypothetical protein"
                     /protein_id="WP_257552557.1"
                     /transl_table=11
                     /translation="MTTDFTTLGTAATAWDAMAGKFESLRTTYEKKVPSTTTSGVWLGQ
                     AQQVAAPRFAVTRNEYSNAQIQARAVAALLRDAETLFTELRGKVKAAVDEAVAAGMKVS
                     EGGNASFDFSKVDAATAKAARHDPDLAEVERSWTRRIQQAVDAFEEADHGVKLALVDAV
                     TGTNFNDGTFGGFNGSRPYPSLAAAAKAESMPKDRAKVADWWRSLDAVTRGILVEEQGD
                     ALKKAGILDPAYQWRQADPGAGGFGTEEPTAHDSYLLAQGEAMLAGGDFIGNTEASRNL
                     AHYLGGTGTTLRLDIDRAMSDDGSFRASIVSDISNHQEQWKQEALDAFEKSGGAPVSIP
                     VETTAVGGTFEDRNWYLGVGSHMRNVSGNVSVVPDADGQPKITMDYQVNVWDRYNWDAG
                     KETPIGPFTVRDTDMARLHTVGLAQEFDMQGSSSAKQHDLGGADAPVVPAGDPGRDGTR
                     KDPSRGEEENR"
     gene            complement(<6922..7125)
                     /locus_tag="JIW86_RS41570"
                     /pseudo=""
     CDS             complement(<6922..7125)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039977.3"
                     /locus_tag="JIW86_RS41570"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF6069 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVLVTGAVGAAVVNLIVLAVATLAGASLVVVDGGAEHPITVGGVI
                     GASVVPLVVGTGAALLMALWKPV"
     misc_feature    complement(7328..7343)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.67"
                     /tool="antismash"
     gene            7392..8891
                     /locus_tag="JIW86_RS04195"
                     /old_locus_tag="JIW86_04195"
     CDS             7392..8891
                     /EC_number="3.1.1.-"
                     /GO_function="GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605510.1"
                     /locus_tag="JIW86_RS04195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04195"
                     /product="lipase family protein"
                     /protein_id="WP_257552559.1"
                     /transl_table=11
                     /translation="MATDANQPTVPQVTMTLSAIAATAATPRPSGETLAEQSARIILGI
                     NQQLADTSLATQGTWVLSWLGVSPGNANMAYLARNTDSSNSFAVVIRGTVGSVTDLLED
                     LDVGTVVPFNLVGMPSSVSVSKGAMKAFTQVVTMSTAGVTLAQALNTALSQAEPNPTVY
                     VTGHSLGGCIATMVAPYLQTLTWPKGKPNFELHTFAAPTAGGANFAAYVDSLDWQANER
                     HFNINDMVPQAWNNLEVTKQWYLTPGPAADDDVKAVISALVALPGPNVYVQPGKDDPLN
                     DSYDPVDPNLVKASLQDFMGQVAFQHANSTYLGLLGAPDVQSGPVVTVVTPVGTAGAPV
                     EIVGSGFGEGVMVDFGTVPISKEYVLRDDVIVVWAPEGVGVVDIRATNNLGTSPAVPRG
                     QFSYGGPAAVVATGISPDTGRAGDQVQINGVNFASDCQVYFGDNLAELVDYAPEQLLVN
                     APKPTIGVGKSATVNVTVLSNGYSSPATPADEFTYLEWHRP"
     gene            complement(9584..9793)
                     /locus_tag="JIW86_RS04200"
                     /old_locus_tag="JIW86_04200"
     CDS             complement(9584..9793)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /locus_tag="JIW86_RS04200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04200"
                     /product="cold-shock protein"
                     /protein_id="WP_010221054.1"
                     /transl_table=11
                     /translation="MSNRQTGTVKWFNDEKGFGFITPQSGDDLFVHFKAIKADGFKSLK
                     EGQAVSFIATRGQKGMQAEEVEVI"
     misc_feature    9872..9887
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.02"
                     /tool="antismash"
     gene            10001..10849
                     /locus_tag="JIW86_RS04205"
                     /old_locus_tag="JIW86_04205"
     CDS             10001..10849
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR04539.1"
                     /locus_tag="JIW86_RS04205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04205"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_257552560.1"
                     /sec_met_domain="CDPS (E-value: 3.3e-16, bitscore: 58.5,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGCRIGSPGSSYVIWRVKERFCARCHQTEQLPLPDNYFRRKAPVD
                     NQTVPSAPTPSSTHPEGRRRTRYRTAIHSVSPAANRNTFEDHEACFLGVSLDNSSFTPA
                     KFDSMLKWVSRHFSRCTVLIGDSIHRLTLASTRGMNPDQALEHALDLGRQFIHAQQPVL
                     DSYRAVTDFTVVRCSQVQETEEYAEFHYELRTLFAKDDAFRASVEAFGRAYHAKHSPRS
                     VPRNATSASIRPAPTSSKSSPSSATSHAATCPSWSTPDSSSPSPKSPPASTPKHRTNSA
                     I"
     misc_feature    11268..11283
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.96"
                     /tool="antismash"
     misc_feature    complement(11291..11305)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            11296..11760
                     /locus_tag="JIW86_RS04210"
                     /old_locus_tag="JIW86_04210"
     CDS             11296..11760
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04210"
                     /product="hypothetical protein"
                     /protein_id="WP_257552561.1"
                     /transl_table=11
                     /translation="MNLTRVRRRIVNTVAASALAAGALLGAAPTASAGEACDGGCSQTY
                     NDTGLSMLVARNWCWGYWDGWQRSGDDAVRQCNSDDKYSLLSPWTRTPGTVDWDTFRVD
                     GGWCYRMYFYNDWTGRKSYYTLDRTGTNGLWVKVANNESAHIYSQTYGHC"
     gene            12078..12395
                     /locus_tag="JIW86_RS04215"
                     /old_locus_tag="JIW86_04215"
     CDS             12078..12395
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04215"
                     /product="hypothetical protein"
                     /protein_id="WP_257552562.1"
                     /transl_table=11
                     /translation="MNVDTMFASCTYAGSRALAGESSDHGGIGWVALTRDDDHNTLEWI
                     AVSCSSNPFTEVTLDATTVTATSTLGRIWAFPRNTPQQVEITEDPAHPWPLPSQTGGPA
                     G"
     gene            complement(12624..13010)
                     /locus_tag="JIW86_RS04220"
                     /old_locus_tag="JIW86_04220"
     CDS             complement(12624..13010)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04220"
                     /product="hypothetical protein"
                     /protein_id="WP_257552563.1"
                     /transl_table=11
                     /translation="MTNRYADEQLAIVAKMVRRTVTRRERARFDKNFDRWMREEWNGSE
                     MRSFVACLAAVSTACGGARGEWGTLAEDEQQAMVHYFGMAYLPTRDEAYADAEEFVESE
                     VRRATVGMKEFAQYLVRMFSDPRG"
     gene            complement(13022..14263)
                     /locus_tag="JIW86_RS04225"
                     /old_locus_tag="JIW86_04225"
     CDS             complement(13022..14263)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04225"
                     /product="hypothetical protein"
                     /protein_id="WP_322975486.1"
                     /transl_table=11
                     /translation="MRALLTGPLCGTWPEERVSFVGNRARLGELPHELVLGLRDAVDVA
                     LFYYVGHGQYDNDDRLCLALGDSSPDAVLRTTTSLTFDAVRQAFRASKAATKIAILDCC
                     FAGLAASQDGRLAGADDMPRSPGFCLLMASGEFSTAWFENTTESPQPQTYFTKYLADLI
                     EHGVPGGPAGLALGPIFQDAAHALVRDGKPPPRSRFSDHAAEFVFARNRAHPQFESSQR
                     SSLPGPSRRTPSGPTVELPAPTGAPADSSALRPDADEMYVAALALEEGSGGAKRLAEIE
                     SLYRTAADLGQPDAMARLGLVVEGRTRARLQGELFGENSDGDIEAAMYWYRKAADHGSV
                     FGAFWLGLLYEDRLGDTSEAMKWYETAARGGHQGARTALDGLRDRLSRGLGPLTRSAGF
                     KGAPRSNHPTGNRR"
     misc_feature    complement(14042..14044)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(14367..14699)
                     /locus_tag="JIW86_RS04230"
                     /old_locus_tag="JIW86_04230"
     CDS             complement(14367..14699)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040404.3"
                     /locus_tag="JIW86_RS04230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04230"
                     /product="hypothetical protein"
                     /protein_id="WP_215149462.1"
                     /transl_table=11
                     /translation="MGEVSLLAEVSVSDPGELRSLREHLRLIPGVDVDQAPGVPGEGEL
                     GVWDVLQVTAVGSGALAVAVRTLPEFIRSRRSSVTVTVKTAGKTVTVTAQNIDEAMPIV
                     EKALDA"
     misc_feature    complement(14778..14796)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.68"
                     /tool="antismash"
     misc_feature    14794..14807
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.92"
                     /tool="antismash"
     misc_feature    complement(15132..15146)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.49"
                     /tool="antismash"
     misc_feature    complement(15132..15147)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.28"
                     /tool="antismash"
     misc_feature    15133..15147
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.49"
                     /tool="antismash"
     misc_feature    complement(15460..15476)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.45"
                     /tool="antismash"
     gene            complement(15685..16116)
                     /locus_tag="JIW86_RS04235"
                     /old_locus_tag="JIW86_04235"
     CDS             complement(15685..16116)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468255.1"
                     /locus_tag="JIW86_RS04235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04235"
                     /product="subtilase-type protease inhibitor"
                     /protein_id="WP_257552564.1"
                     /transl_table=11
                     /translation="MSSVVRHRIAAVSTATLLTLAGATGLAEAQPASLYAPSAVVLTVA
                     QGEYADTVVRASTLSCAPTAQGTHPDPKAACAALAATAGTLDELLAAPDSNRACPMHYA
                     PVTVTADGVWQGSRISWKHTFSNACTMSATFNGNTVFAF"
     misc_feature    16107..16121
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    complement(16195..16208)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.8"
                     /tool="antismash"
     gene            complement(16710..18254)
                     /locus_tag="JIW86_RS04240"
                     /old_locus_tag="JIW86_04240"
     CDS             complement(16710..18254)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04240"
                     /product="DUF6056 family protein"
                     /protein_id="WP_257552565.1"
                     /transl_table=11
                     /translation="MQARKPIARPAGQTSAAGTGPGPAGPPGKSAGAGLLRGTGGVLTA
                     AAGALLGIAGFLGTFLRPTSDDWCMAWKTRDMGVLGVTSDFYLTQNGRITNAFLSGVVY
                     SHGMLGPQVLSTVLIIALGLGLYLLAREMLGALDVRAPLAVLAPAVLVVEALLFLGGTR
                     TYQMLWAPAAISHILPSVLGIWALLAAIRAARSPRRRPRQAALVGALVTGFAIGTLSEP
                     FLAVSGVTAATAALLTLPRLPLALRLARTWYPFTWSTLYCAGLTAGLAVLYTSPGAAWR
                     RAQRPPREFSARRLARDWVHIWDTILSQWAYLGAAAAGVLLGLALTLTSSQPQPPVTRR
                     RGLPQPAARLLLILPIPVLILSSLAVAHGLRLGYGGNGWTYARTWTSFLIPYLATLTLY
                     GALLGHWVGQRAHTPDKPRLPRAAALLAGTFTLAALAALIPAAQQLTSQTVTRALRWDA
                     QDARIRDEVTEGAGSVTYGAMPIGSLAEPYFSRQEGKDWVAPCTAKYYQVQQIHKP"
     gene            complement(18344..18949)
                     /locus_tag="JIW86_RS04245"
                     /old_locus_tag="JIW86_04245"
     CDS             complement(18344..18949)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS04245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_04245"
                     /product="hypothetical protein"
                     /protein_id="WP_257552566.1"
                     /transl_table=11
                     /translation="MEGDAQLAAVAAQVRTALGPAQPPKSPEDAVGAQTAAVLRECADA
                     SASALHARLIAAARTAAPGIRITVHGSASPWATGAFAPLGPRVAEGIDAVVANCWAADS
                     GSGSGSRPEPGPASGGGPVAALRDLAPPATAVGGYFRPETLPAGLGEAHLYHLGLVPLA
                     GPRRRPAARVSRTVCGNGSSESHANDHGTSPDRSPGRP"
     gene            <18920..19351
                     /locus_tag="JIW86_RS04250"
                     /old_locus_tag="JIW86_04250"
                     /pseudo=""
     CDS             <18920..19351
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436191.1"
                     /locus_tag="JIW86_RS04250"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04250"
                     /product="pectate lyase"
                     /pseudo=""
                     /transl_table=11
                     /translation="DRGKLRVTLHHNLFENVVERAPRVRFGKVDAYNNHFVVPGSAHSY
                     SLSIGIESQLVAEKNAFTLADGVQAGEILKKWKDAPVTTAGNFVNGRQVDLLAAHNAQF
                     PGEILRADAGWTPTLRTRVDVPQAVPALVDHRAGAGRLH"
     gene            19543..20346
                     /locus_tag="JIW86_RS04255"
                     /old_locus_tag="JIW86_04255"
     CDS             19543..20346
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007453013.1"
                     /locus_tag="JIW86_RS04255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_04255"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_257552567.1"
                     /transl_table=11
                     /translation="MHMYKLRWVVPSLLTLVAATLGLTHPASAATAAGERYVALGDSYS
                     SGVGAGSYLSDSGSCQRSSKAYPSLWAAANAPASFSFTACSGATTSTVKSSQLTPLNSS
                     TTLVSVTAGGNDVGFANVMQTCVLQSESTCVNAVNNAIAQVNNTLPGSLSSLYGTIRSK
                     APQAHVVVLGYPRFYQLNGSCIAGLSETERAAINRGSDALNGMLAKQAANAGFSYSSVV
                     DEFTGHEICSGDAWLHSVSVPVSNSYHPTATGHASGYLPAFRSAV"
     misc_feature    complement(19550..19564)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    19579..19581
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(20581..20596)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.32"
                     /tool="antismash"
ORIGIN
        1 tgtgtccttc ccggccgcgt accgcgcgta tctcaaagag acgaagcccg ccaagccggt
       61 gttccggccc cgtcgaaaag agtggggctg ggattggggc gcggaccgtc agaccctggc
      121 agagctgctg cccctgccct ttccacaccc cgactcattc gtgaacgcga acgccgaact
      181 ggacgcacgc gaaccccgcc tggaggactt caccaacggg caggcccacg cagttgcctg
      241 gcaggcctgg gacggggaat gcgaggtctt cgaagaccag aagaccgccg gagccgtgat
      301 cctgcaggac cacggctgcg gcttctacac gctgctggcg gtcaccggtc cactggccgg
      361 cacggtgtgg tgggatggtc gcgccacctg tgacctgatc atcccgctgt ccctcgatca
      421 catgggcagc gcccgcccgg tcacgttcag cgagtggctt cgccacgatt cctgggactt
      481 gctgccgccg ggctggggta ggtcccctcg ctccacctca taggccgatt tttgggatca
      541 cctagctgac cagatcatga cggcggcgag gtggagccgt gggatcgaag gtcggcgaga
      601 gccgaccttc gcgggcgcga gccgagcctt ggagtatgcc cgggcctgat ggccctgttg
      661 acaagacaag gctggctcgc gctcggcagt gtgggcatga agcgtgcaga acccggtgcc
      721 gtgttgtcgg catggccatg gaaacggtcg tacgcccggg agcaaggctg atgcccatgt
      781 ctggcgcgag tcggacctgc cggccgcggc ggccgggacg gcggcgatcg cggacggcgc
      841 ctacatcggc accgggctcc ctccaaggcc gtcggccact tctccagcct caaggggagt
      901 tggtcggcgg cccatcagag cgaagcgcgg tccgcaactc atcgggtgtt gcaggccctg
      961 ggtcacgcac aggctgaccg atgtcggatg accagttggg acggaagcgt tcgacgaggg
     1021 ccgtgaagtc attccgcagg taccgagaat cggacggcat cagccggatc tgttggcggc
     1081 tgtcagccca ccagatctcg aaatcggcaa ccgtgccttc cgccggccag tcctcactct
     1141 cgtccggcag ccacagggcg tcgacgaagc ctccgacgct caggccgatg tccacgaaga
     1201 tcccaatcgc accgggccgg ggaacctgcg ccacagtgcc ctcgaagacc tggccaaagc
     1261 gcaggcccct ccgaatccga gcccactcgg catctgtcac cacgaacgaa tgatcgcact
     1321 ttcgtccgga tcgtgaacag ctggcgctca aggtggactg cacgcagcgt cccgtcacca
     1381 taccgatcga cctgtccgcc tcggcgacac ggctctgccg ttacttctcg ggttctggca
     1441 caccttccgt gagcgatctc gctgcctctt cgacagcagg atgtgcacac cacgtgagct
     1501 gtgcggattg gatgtcacgg gaagccggtg gagcctcagc cgagatcttt ttcagcatcg
     1561 ggatgctcct ggtgaaaggt gctggccatg ccgcaaatgc gtccagcacg tgcagcacgt
     1621 ccttttcgtc cacaggcagc aaggtcgagc acgagcactc gggggccctt ccacgatgcg
     1681 cttcctgtga aagcgcaggg cgcccgcgag ttggcgggat ggtcagccca ttggggccaa
     1741 ctaccggatg atcacaggag gttcacaggt ctaccacggt gattgtggtt cactaccggg
     1801 gcgcgacagg acataaacga tctgtcaact ggaaggcata gaaccggtgc atatacgacg
     1861 cacctgcaaa gctgccaccg ccttactgtt cgcggtggcg ctgatacctg ccaccctcac
     1921 gaccgcctcg gcgtccgagg cacccacgcc cgccgtgccg ggcgtgacgt tcggcaagca
     1981 gaccaccgat ccgcagaagg tccgggactt ctggaccccc gagagaatcg cccgcgcgct
     2041 ggcgaacgag aagacggacc aggccggcat cgcccgcgac gccgcagtcg ccaaggcagc
     2101 gggcgaggcg cccgaggacg cggcaaggag caccgccccg cgactgacgg ccgaggctcc
     2161 gcagatcctc cccgcgaaga aggcgagcgt gtcgacgatg ggggcggcgg ccgacgtccc
     2221 cgagatgccc gtcgcacaga aggttccgtt cccgcagaac gcgcccgcca ccgtcgtcgg
     2281 caagatcgtg ttcatcgacg acgcgggcaa ggaacacggt tgcagcggcg cctcgatcgc
     2341 ggctgacggc aacaacaccg tctggacagc gggtcattgc gtgcacccgg gcgacggacg
     2401 cgggtccgat ggcttctacg acctggtgct cttcattccg ggctacaagg aaagccttga
     2461 cacccctggt gactacgatg cgccgtgggg cgagtgggtc gcccagtcgt tcgtggcccc
     2521 gcaggcgtgg acccgcgaca aggactacga cgagggcgac ttggccgcct tcaccgtcaa
     2581 ggccccgacc ggctacacga acctcagcga caccgtgggc gcgctcggct acaagttcgg
     2641 gtacggctcc gactggtcgg acatcatcga ctccgggttc ccgggcgagg gctacaaccg
     2701 caccgacatg gacggctaca cgcagttcta ctgcaccggc gacgtcgtgg acgcggtgga
     2761 ctggtacccg tgggacaacc ggctcgagat gaactgcgac atgggagcgg gcgcgagcgg
     2821 cggacccatg gccaccaccg aggggcagat cgtcggagcg aactcccacg tggacaccga
     2881 cgatcaggat cagcggatca acgatcacct gtactcgtcc aaccacggcg accaggccgt
     2941 agcggtgatc aacgcgatca acgacgcgaa ctgatccacc cggcgcacag cgggccccgg
     3001 cccaccaggc cggggcccgt acctacccgt ccacgcggcg gcccatgtcc gggtcgatgt
     3061 cctcggcagt cggagcgttg agtatccgct ggtgcaggta gcggccccgg atctcatccg
     3121 tggtcagcac ttcccacccg tccggcagct ccttgcccgc atgctcgagc tcggccgtcc
     3181 acgccttgac gagtccagcc cgagtctccg gtaccttcga ctccgccggc tgcggcatgc
     3241 cgaccgtcac cggaggcaac tcgtcctcct gaacagccgc caccgcgaac cccagcgcgc
     3301 cgcccgccac gaacgtcgcc gcggctatgg cccacacctg cctgcgtgtc atcttgctcc
     3361 cctcgcgatg cgcacccacc tgtaccgtgc cacagccggg cgaggagggc gacaaccggg
     3421 gatcgaccgg ctcaccccca acggcaccat cttcgagacc ggcacggact cctaccgcct
     3481 cggccggcac cggggcccga gccgatcaga ccgcagtggg cacgcccgtc tgcgtccaga
     3541 cctggcgtga cctcgtcgaa gccactgcgg cagccgtcac gctctggctc cgaacatgag
     3601 cgacgggcaa gctagtcggc gacgaccccc gtgttttggc aggcggtcaa caaccagcgg
     3661 ccgtcggctt gcttcgagat cacgtacagc ggggctccct cgctctcctc gcccggagcc
     3721 aggtaacgct gacggacctt gactgccgcg acgtcggggc ggatgaagag cacgtggatc
     3781 acctcgtagg tgaccttgcc gtcccaggtc gccgcgcgga ggactttgcg ggtgaactcc
     3841 gagatcgcgt cgaggccgat gaggaccttg ccgtgggccg tcgtccagat cgcgtccggg
     3901 tggaacaggc cgaggaactc ctcggggtcc ttgttctgct gggagcgctc gacggtggcg
     3961 acgagccggc tgatcgcttc gatatctgcc gcgtgcgatg tggattccgt ggtcatgcgg
     4021 atcatcgtca cacctcaagc gagattgagg tcaagcgtgt gatcacatgg cgatgtgaga
     4081 tcccttcttc caccgggccg ggcacggtga cgcccgggcg gtcgtgcacc acctgactgt
     4141 cgacgcacgg gtcaaagacc cggctctgca catcggcggg tacgccgggg ccggtaccac
     4201 ggaactcgac tcggtcgtag tcgcgcacga ccgtcatgtc cgggacgatc ttcgctgcga
     4261 gcagcaaacc cgaaggggac cgcgccgggc aggttggtga cctgcggggt gtcggacgcg
     4321 gcgccgaagg gcgtaacggc gaaaacgtcg aggttacggt gccgtccgcg cagagaagtg
     4381 ccgacgggcc gcctgaaacc agccgtctgc cgagtcctgt gaataggctc ttgtcgccac
     4441 caaggtgacc ggagccgagt acgcgagtgt ccgccagtgg gccgcggaac ccagagcacc
     4501 accttggttc cgcgtggtgg tgcatcccgg cgccatatca cccggtggag acgcgcgtgc
     4561 atgctccccc gcactcgtgg gatgtccccc accgtggacg tccaggcgat ctgcgagtga
     4621 cgtcagaatg ccttcaggta gacgcgtaca cggccgtcgc cggtgtcttg gatgaagatg
     4681 gtgatggact ggacggtgga cctgcgctcg tccttgatgc agggcgggca caccgaagcg
     4741 gttcggacct tctgctcggt ttccggctcc ggcagcccga ggtgggacca cggggtaatg
     4801 gttgtgtacg ccttggggtc gcgcggggtg ttggcccgca gggcttcctg cgggcgcagg
     4861 tcggcgatga acgtctcggc ctcggctgcc gatgccgtga aggaaatcag gtagatggtg
     4921 tcctggaagc cgcgttgcac agcgcctttg acctcggtgg cgccggtcgg tgtctggacg
     4981 cgcatgaacc cggcggcctc gggagcacct tggccctcga cccagacggg tcccccggtg
     5041 cgggccttcg gtgtgccatc gtcgctacag gcggtggccg acagggccag cagaatcgtc
     5101 accgccacag aggtgatgcg gttgaagagg gacctcgtag ccttcaccgg ttttcctcct
     5161 caccccggga ggggtccttg cgggtgccgt cgcggccggg atccccggca ggcaccaccg
     5221 gtgcgtcggc gcctcccagg tcgtgctgtt tggccgaact actgccctgc atatcgaatt
     5281 cctgcgccag accgacggtg tgcaggcggg ccatgtccgt gtccctgacg gtgaacggcc
     5341 cgatgggggt ctccttgccg gcatcccagt tgtagcggtc ccacacattg acctggtaat
     5401 ccatggtgat cttcggctgg ccgtccgcat cgggcacgac cgagacgttc cctgagacat
     5461 tgcgcatgtg ggagccgact ccgaggtacc agttgcggtc ctcgaaggtg ccgccgaccg
     5521 ccgtcgtctc cacagggatc gataccggcg cgccgcctga cttctcgaac gcgtccagtg
     5581 cctcctgctt ccactgctcc tggtggttgg agatgtccga gacgatcgac gcccggaaac
     5641 tgccgtcgtc gctcatggcc cggtcgatat cgagccgcag cgtcgtaccc gtaccgccca
     5701 gatagtgcgc gagattacgt gaggcctcgg tgttcccgat gaaatccccg ccggccagca
     5761 tggcctcgcc ctgcgcgagc aggtaggagt cgtgcgcggt gggctcctcg gtcccgaacc
     5821 cgcccgcgcc agggtcagcc tggcgccact ggtaggcagg gtcgagaatc ccggccttct
     5881 tgagtgcgtc gccctgctcc tccaccagta tgccgcgcgt caccgcatcc aggctgcgcc
     5941 accaatccgc gaccttcgca cggtccttgg gcatactctc ggccttggcc gcggctgcca
     6001 gggacgggta ggggcggctg ccgttgaacc cgccgaaggt gccatcgttg aaattggtgc
     6061 cggtgacggc gtcgacgagc gcgagcttca ccccgtggtc ggcctcctcg aaggcgtcca
     6121 cggcctgctg aatccggcgg gtccaggagc gttccacctc ggccagatcg ggatcgtgcc
     6181 gggcggcttt cgcagtggcg gcgtcgacct tggagaagtc aaacgaggcg ttcccgcctt
     6241 cgctgacctt catcccggcg gccaccgcct cgtccacggc ggccttgacc ttgccgcgga
     6301 gctcggtgaa gagcgtctcc gcgtcacgga gcagggcagc aaccgcccgc gcctggatct
     6361 gcgcgttgct gtattcgttg cgagtgacgg cgaagcgggg agcggcgacc tgctgggcct
     6421 ggcccagcca tacaccgctg gtcgtggtgg acggcacctt cttctcgtac gtcgtcctga
     6481 gagactcgaa cttccctgcc atggcatccc aggccgtcgc ggccgtgccc agggtggtga
     6541 agtcggtagt catgacttcg gggtaattca gcatcagctt cgcccctgcc cgcccagaag
     6601 ctgctggtat ccgtccctga cggccagatc ctgccgctgg aagaggaggc cggaaccgct
     6661 cagtgcattg gcctcaccgg taagccgccc caagagcagc ttgacctgac ccgaccaggt
     6721 ctcgtcgacc gttttcaggg cggcggccgt gctcctaccc ccggcttccc accggccgcc
     6781 acggacgagg tcctcgacga cacggcggac ctggacggcg gtgacctcgg cgacatcgcc
     6841 ctcggggccg aggcggaccg cctccagcag ctggcgccag gaggccaggc caccgcccac
     6901 gtactgcccg atccgcagga agacgggctt ccacagggcc atcaaaaggg cagcgcccgt
     6961 ccctacgacc agcgggacga cggaggcgcc gatgacgccc ccgaccgtga tcgggtgctc
     7021 tgcgccgccg tcgacgacga ccagcgaggc accggccagc gtggcgaccg caagaacgat
     7081 cagattgacc acggccgcgc cgaccgcgcc ggtgaccagc accatccacc agccgaggcc
     7141 ggaacggcgg accgagggca cggcggacac ggcgggtaca gcggggccgg agtttgccgg
     7201 ccctcccggc cctgcgaggt cagaacacaa agaacagctt ggaacctgtc cgccgaacag
     7261 gaacaacccc aggggctgct ggcccgatcg cgtgaacccg ggacccgcct cccgtcggga
     7321 tcccacggtg aaaagtacgt tggacgcgaa cctgctccgg ccggaccggg cgatcccaga
     7381 ggagagcgag catggcgacc gacgcgaacc agccgaccgt cccgcaggtg accatgacgc
     7441 tctctgcgat cgcggcgacc gccgccaccc cgcgcccctc gggcgagacc ctcgcggaac
     7501 agagcgcccg gatcatcctc ggcatcaacc agcaactggc cgacacctcg ctcgcgaccc
     7561 agggcacctg ggtcctctcc tggctcgggg tgagtccggg caacgccaac atggcttacc
     7621 tcgccaggaa caccgacagt tccaactcgt tcgccgtggt gatccgcggc accgtcggca
     7681 gtgtgaccga tctgctggag gacctcgacg tcggcacggt agtcccgttc aacctcgtcg
     7741 ggatgccgtc ctcggtctcg gtctccaagg gagcgatgaa ggcgttcacc caggtcgtca
     7801 ccatgagcac ggcgggtgtc acgctggcgc aggcgctcaa cacagccctg agccaggcag
     7861 agccgaaccc gacggtctac gtgaccggcc acagcctggg cggctgcatc gcgaccatgg
     7921 tcgcccctta cctccagaca ctcacctggc ccaaggggaa gccgaacttc gagctgcaca
     7981 ccttcgctgc ccccaccgcc ggtggagcaa acttcgccgc ctacgtcgac tccctggact
     8041 ggcaggcgaa cgagcgccac ttcaacatca acgacatggt gccgcaggcc tggaacaacc
     8101 tcgaggtgac caagcagtgg tacctcacac cgggcccggc agcggacgac gacgtcaaag
     8161 cagtcatctc ggcgctcgtc gccctccccg gtcccaacgt ctacgtccag ccgggcaagg
     8221 acgacccgct gaacgacagc tacgaccccg tcgacccgaa cctggtgaag gcgtcgctcc
     8281 aggacttcat gggccaggtg gccttccagc acgccaactc cacctatctg ggcctcctcg
     8341 gcgcgcctga cgtgcagtca ggtccggtgg tgaccgtcgt gactcccgtc ggcacggccg
     8401 gagcccctgt cgagatcgtc ggcagcgggt tcggcgaggg tgtcatggtc gatttcggca
     8461 cggtgccgat ctccaaggag tacgtcctcc gcgacgacgt gatcgtcgtc tgggcacccg
     8521 agggcgtcgg cgtggtcgac atccgggcca ccaacaatct gggcacctcc ccggccgtcc
     8581 cgcgcggcca gttctcctac ggcggccccg ccgccgtggt ggccactggc atcagcccgg
     8641 acacgggccg ggccggcgac caagtccaga tcaacggcgt caacttcgcc tcggactgcc
     8701 aggtctactt cggcgacaac ctcgccgagc tcgtcgacta cgcccctgag cagctgctcg
     8761 tcaatgcacc caagccaacc atcggcgtcg gcaagtccgc gaccgtcaac gtcaccgtcc
     8821 tctcgaacgg ctactcgtcc cccgcgaccc cggcggacga gttcacctac ctggagtggc
     8881 accggccctg atccggttcc tgacccctaa ggcgatatcc gtgggagaac ctcacggccg
     8941 gccgacagca gctgcctgcg gattgaccga aaacaccgcc tccacgtgaa gctgcggtgg
     9001 aagtggccgc cggcgaacca gtggaaggca atctccgcac gggtgtcact ccgccaggtg
     9061 gccgcctcct gaagtgacgc cttcgagccg gcctgcccta catggttgcg tacggcaagg
     9121 gcacggtggc tgagatgcag cgggccacgg gcatcggctg gacagacgtc catgagacgc
     9181 gtgcttctcc cctggtctcg catgtggtcg acagcgacat cgcgacagtt caggcagcga
     9241 gtcccgatgg cctgtcatcg ccatgtgctt tgtcgccgga tatggcgctg ggccgaggaa
     9301 cggggcctgg cgcgcgttgc gcttcccgcg gttgcggggc agtaatgccg cagcggtccc
     9361 gcgcctgaag cctgagggac tccggaccga acctgcgttg ctggggcgcg gccaagcaac
     9421 cactccctgg ctcggagtgg gccacccacc tggcgcagct gtcacgcatg aacggtcggg
     9481 ccagccgcgg cgccaggagg acaccggacc gttgtgactc gcggcagccg gccattctgg
     9541 cgagctgccg cgcacagtca cgggtagcgg ctcgggtgga gggtcagatg acctcgactt
     9601 cctctgcctg catgcccttc tgcccccggg tggcgatgaa cgagaccgcc tgcccctcct
     9661 tgagggactt gaacccgtcc gccttgatgg ccttgaaatg cacgaagaga tcgtcgccgg
     9721 actgcggcgt gatgaagccg aagcccttct cgtcgttgaa ccacttcact gttccggtct
     9781 gccgattgct catgtccacc ccaagggagt cgtggcggcc tcgtgccacc gatccgatct
     9841 tggcccgtcg tgaccggatc cacacaggat gccgcttgga gcgaacaccg cacatccgtg
     9901 gcaagccgct ttcggagtga gcgaagccgt gcgggccgcc acccggtgtg ggtggcggcc
     9961 cgcccctcag cggggtgacg gcactcagcc ctgctgcggt atggggtgtc gcatcgggtc
    10021 accaggctct tcgtacgtga tctggagggt gaaggaacgt ttctgcgcca gatgtcatca
    10081 aacagagcaa cttcccctac cagacaacta cttcagacga aaggctccgg tggacaacca
    10141 aactgttccc tctgccccta ccccctcctc cacgcacccg gaggggagac gccgaactcg
    10201 ctacaggacc gcgatccact cggtctctcc cgcagccaac cggaacactt tcgaagacca
    10261 cgaggcgtgc ttcctcggcg tcagcctgga caacagcagc ttcaccccgg ccaagttcga
    10321 cagcatgctc aagtgggtct cccgccactt cagccgctgc accgtgctga tcggcgacag
    10381 catccaccgg ctcaccttgg cctcgacccg cggcatgaac cccgaccagg ctcttgagca
    10441 cgcactcgat ctgggccggc agttcatcca cgcccaacaa ccggtcctgg attcctaccg
    10501 tgcggtgacc gacttcaccg tcgtcaggtg cagccaggtc caggagaccg aggagtacgc
    10561 ggagttccac tacgaactgc gcacactgtt cgccaaagac gatgccttcc gcgcttcggt
    10621 ggaagccttc gggcgcgcct accatgccaa acactcccca cggtcagtgc cgcggaacgc
    10681 caccagcgca tcgatacgtc cagcgcctac ttcctcgaag agttcgccgt cttctgctac
    10741 ctcacacgcc gcgacctgcc cgtcatggtc taccccggac agttcgtcac cctcgccgaa
    10801 atcgccgccg gcctccaccc ccaagcaccg gacgaactcc gcgatctgac cctcgtctcc
    10861 ctacgcgtcc aaggccgata gcgtcccctc cctcacccct ggcgagcctg gcaaagcgct
    10921 gcgaatcccg gcccagcgga atgcggtggt gagcgtgacg gtcgtgggca tccccacggg
    10981 aacggcggcc tcggtgaccg tggccgtcgt ccccacggcc ggcccaccgg ataaccgtcg
    11041 aggcgcagtt cggctaccgt cccgacagcg cacgcaacat cctggggtgc cgggaacggt
    11101 gtcaccgggc gcaaacggca tccccagaag gcgaaacgcc gcaagcagcg tcgtcccaac
    11161 ccggtggaac gctgcaggtg acggccctga aaacgtccca ctgtcccgga tccggcgctg
    11221 gtccttgccc gctgccctcg gccgatggga cgttcacccc gacccactcg aagttctgtg
    11281 ccagggaggc cgaaaatgaa tctgacccgt gtccgccgcc ggatcgtcaa tacggtggcg
    11341 gcttccgcac tcgccgccgg agcactgctc ggtgcggcac ccaccgcgag cgccggcgag
    11401 gcgtgcgacg gcggctgctc ccagacctac aacgacacgg ggctcagcat gctggtcgcc
    11461 cgcaactggt gctgggggta ctgggacggg tggcagcgct cgggggacga tgcggtccgc
    11521 cagtgcaaca gcgacgacaa gtactccttg ctgtccccct ggacgcgcac gcccggcacc
    11581 gtggactggg acacgttccg ggtcgacggg ggctggtgct accggatgta cttctacaac
    11641 gactggaccg ggcggaagtc ctactacacg ctcgacagga ccggaacaaa cggtctctgg
    11701 gtcaaggtgg cgaacaacga gtccgcgcac atctattccc aaacatacgg ccactgctga
    11761 acgaccccgc acccagggcc ggcaactcat cggccaccct gcgcggttga ccgttgcagc
    11821 cctctcctcg cgccgttcct cgatgcaatc attgcgggtg tgagctcccc cgacgcgaca
    11881 cccaacgctg gcctggtgca tgaccgttgg cgtgcccggg aggcgcccca cggagacttc
    11941 atcatgttct ccgacggatc cctgtcggtg atgagactcg agcggctctc cccgcaggcc
    12001 agcgccatga gacgcgggcc gagaactggc agtggtggga ggtcctacgg gccaccgagt
    12061 ggacccccag cgactggatg aacgtcgata cgatgtttgc ctcatgcact tacgccggaa
    12121 gccgtgccct ggcaggtgaa tcatcggatc acggcggtat cggctgggtc gccctgactc
    12181 gggacgatga ccacaacacc ttggaatgga tcgcagtctc ctgctcgtcc aatccgttca
    12241 ccgaagtcac cttggacgca accacggtga ccgccaccag caccctgggc cgcatctggg
    12301 ccttccccag aaacaccccc cagcaggtgg agatcaccga ggacccggcc catccctggc
    12361 ccttgccatc tcagacaggc ggtcccgccg gctaaccggg gtgcggttcg cggcgggtga
    12421 gaagaccgtg gtgagcgcga aggacttgtg gatgagcctg aggtcggtgg aacgccggcg
    12481 ccgggcctgg cgcgagggaa gcatggaggg cctgcacacg gtcggaccgg ccaacgcacc
    12541 gaccgtcgcc gatgcccggt gcgacctgct cgacctgctc gaccctatgt ataggcccgg
    12601 caggctggct tgctcatggg cggttacccg cggggatctg agaacatccg gactaggtac
    12661 tgggcgaatt ccttcatgcc aactgtggcg cgacggacct cggattccac gaactcctcc
    12721 gcatcggcat aggcttcgtc cctcgtcggc agatacgcca tgccgaagta gtggaccata
    12781 gcctgctgtt catcctcggc gagcgtcccc cactcgccgc gggcaccgcc acacgccgtg
    12841 gaaaccgcgg ccaggcatgc gacaaaggag cgcatctcac tcccgttcca ctcctccctc
    12901 atccaccggt cgaagttctt gtcgaaacga gcgcgttccc gccgcgtcac cgtacgcctc
    12961 accatttttg ccacgatggc gagctgctcg tccgcgtacc tgttggtcat gccgatccct
    13021 tctaccgcct gtttccggtc gggtggtttg aacgtggggc ccccttgaag ccggccgagc
    13081 gggtcagcgg gccgagcccg cgtgacagcc tgtcccgcag accgtccagc gccgtcctcg
    13141 cgccttggtg tccgccgcgg gcggccgtct cgtaccactt catcgcctcg gaggtgtccc
    13201 ccagccggtc ctcgtacagg agaccgagcc aaaacgctcc gaacacgctg ccgtggtcgg
    13261 cggctttcct gtaccagtac attgccgctt cgatgtcccc gtcggagttc tcgccgaaga
    13321 gttctccttg gagccttgcc cgtgttcttc cttcaacaac gaggccgagg cgggccatcg
    13381 catcgggctg tcccagatcg gcggccgtac ggtacagcga ctcgatttct gcgagtctct
    13441 tcgcaccgcc cgaaccttct tccagggcaa gggcggcgac gtacatttcg tcggcgtccg
    13501 ggcggagtgc ggaactgtca gccggtgcac cggtgggcgc ggggagttca accgtcggcc
    13561 cggatggtgt ccgtcgggac ggtccgggca ggctggaccg ctgtgaggat tcgaattgcg
    13621 ggtgggcgcg gttccgggcg aagacgaatt cggctgcgtg gtcgctgaag cggcttctgg
    13681 gcgggggttt gccgtcgcgg accagcgcgt gggcggcatc ctggaagatc gggccgaggg
    13741 cgaggccggc cggtccgccc gggacgccgt gttctatgag gtcggccagg tacttggtga
    13801 agtaggtctg gggctgtggg ctctccgtcg tgttctcgaa ccaagcggtg ctgaactcgc
    13861 cgctggccat caggaggcag aatcccgggg agcggggcat gtcgtccgct ccggcgagcc
    13921 tgccgtcctg actggcggcc aggccggcga agcagcagtc cagtatcgcg atcttcgtgg
    13981 ccgctttgct ggcgcggaag gcctggcgca cggcgtcgaa ggtcaggctg gtggttgtcc
    14041 gtaagaccgc atccgggctg gagtcgccca acgcgaggca gagcctgtcg tcattgtcgt
    14101 actggccgtg gccgacgtag tagaacaagg cgacgtccac tgcgtcgcgc aatcccagca
    14161 cgagctcgtg tggcagttca ccgagcctgg cccggttccc gacgaaggac acccgctcct
    14221 cgggccaggt gccgcacagc ggcccggtca gcaaggcccg catccgctgg aggctgtgct
    14281 ccgctgcggg tactggtcgc aggtggcggt agtcccaagt gcccatcagg acagcccgtg
    14341 accgcgagaa gtcacggtcc aaggcgtcag gcatccagcg ccttctccac gatgggcatc
    14401 gcctcgtcga tgttctgggc ggtgacggtc accgtcttcc cggccgtctt caccgtgacc
    14461 gtgacgctgg aacggcgtga gcggatgaac tccgggaggg ttctgacggc cacagcgagc
    14521 gccccgctgc cgacggccgt cacctgcagg acgtcccaga cacccagttc cccctccccg
    14581 gggacgcctg gagcctggtc gacgtccacg ccggggatca gccgcagatg ttcgcgcagc
    14641 gaacgcagct cgcccgggtc cgacacggac acctcggcaa gaagtgaaac ttcccccacc
    14701 agcctgcccc ctcacttctt caccagatga cgtaccggac gggaccgtta ccgcctcaac
    14761 cacgatccgg gccagctggc ataccgtcaa ctctttctag aaaagaatct agaaacagcg
    14821 tcctgcgctg tccgcgacgc agagcaacag gatcagccgc gggctgccgt tccccgtttg
    14881 ccgccctcgc cccggttgtc ccgggccagg acacggtcct gctggcgggg gcgaggcgga
    14941 tcgcaggagt ggggcgggct gcggtggtga ctgaggtttt cgggttgtcg tcggatggcg
    15001 agcccgcgag ctggccaacc ttgtcgccgg gctctcggcc agggcccggg aaacgatccc
    15061 ggccaccgag cggttcaccc gccccgagct cgcccccgca agcggggagg cgccccctaa
    15121 gcccgcagtc ccggaccaat tggtccggga ctgcccgccc cctgcgcttg cgccatctac
    15181 tgtgggccgc cctcggcgtt gccaccccga cggtgcctgc cgtaaccagc ctcgtccggg
    15241 tggctggcca gccggacagc ttcccaggct gcgtctgcgc ccaggcccgg cccggactcc
    15301 gccatgaggc gtctggcccg ccaggccgta cgcggagtga gaacccaccg ggcaaaggac
    15361 aacccgaggc cgaccgtggt gcgatcagcc ggccgcttcg catgccggcc gcgggtattc
    15421 ggggcgcgct catccggagg tggtgatgtc tctgccacgt cgcgagtgtc acgcaagagc
    15481 cttccggagt cggccgaagc acgggaagac caccggattg agtgagcgca ccgcggagct
    15541 ccctgccgat cggcagatgc ggagcgacgt accgccattc ctcgtccgtc aactgcacgc
    15601 gcgtcctgtg gcagttcgcg cgtccgcccc cgcccgggga ggagcggggg cggacgcgca
    15661 gggcggacga cccaggagga gcggtcagaa tgcgaagacg gtgtttccgt tgaaggtcgc
    15721 ggacatggtg cacgcgttgg agaaggtgtg cttccaggag atgcggctgc cctgccagac
    15781 gccgtctgcg gtgacggtga cgggggcgta gtgcatcggg caggcgcggt tcgagtccgg
    15841 ggcggccagg agctcgtcga gggtgccggc ggtggcggcg agggccgcac aggcggcctt
    15901 ggggtcggga tgcgttccct gggcggtcgg cgcgcagctg agggtcgacg cccgcacgac
    15961 ggtgtccgca tactcgccct gggcgacggt caggaccacg gcggaggggg cgtacaggct
    16021 ggcgggctgt gcctccgcga ggccggtggc gccggccagg gtcaggaggg tggcagtgga
    16081 gacagcagcg atgcggtgac gcacgacgga actcactttc aattcgaagg gaagaagcag
    16141 cagttggctg cgatatgccg actggacacc gctgagtctg tcgggtgtga agggtgcatg
    16201 cacatcgaac gccgatttac agacatctcg ttcgattatc ggcgaccgaa cggtcgttca
    16261 ggcagctcgg accgggtgcg tgcccttcgt gatcacgcac aaacggacgg tgacagccgg
    16321 cgggacagcc gtacgtgggg cgttgacctg ctgttggctg cgcgcggttc atcgaacgtc
    16381 tatccaggct gaccggatag tgccggtgcc tcccggtgcc tcccggggga cgcgggtggt
    16441 caagggtggc cttcgtggtg tggcattgga aggccgagaa gcaggatgcg tcgccgccgc
    16501 tgccgcacgc tcgcgggcgg cgccggtacc gatctgccgc tgctgtgctg gggcttcagg
    16561 tacggcatgg ccggtgcggg gagggcgaag gcgtgagcga cgaccggcag caccggggac
    16621 tgctcgatcg tccgcccgtg cttcagggcg ccaatgaccc ttcgccttcc ctgccaagtc
    16681 cagcctgttg atccgacgtt ggagtcaggt cagggcttgt ggatctgctg gacttggtag
    16741 tacttggcgg tgcacggcgc gacccagtct tttccttcct gacgggagaa gtagggctcg
    16801 gcgagggatc cgatgggcat cgccccgtag gtcacgctgc cggctccttc ggtgacctcg
    16861 tcgcggatac gggcgtcttg ggcgtcccag cgcagtgccc gggtgactgt ctgagaggtg
    16921 agttgctggg cggcggggat cagggcggcc agggcagcga gagtgaaggt gcccgccagg
    16981 agggccgctg cccgagggag acgtggcttg tcgggcgtgt gtgcgcgttg tccgacccag
    17041 tggccgagga gtgccccgta cagggtgagg gtggccaggt acgggatgag gaaactggtc
    17101 caggtgcggg cgtaggtcca gccgttgccg ccgtatccca gacgcaggcc gtgagcgact
    17161 gcgagggacg acaggatgag gacggggatc gggaggatca gcaggagccg tgccgccggc
    17221 tgcgggagac cacgccggcg ggtgacgggt ggctgcggtt gactggaggt gagggtgagc
    17281 gcgaggccga ggagtacgcc cgcggcagcg gcgccgaggt aggcccactg gctgaggatg
    17341 gtgtcccaga tgtggaccca gtcgcgtgcc aggcgccgtg cgctgaattc tcgtggtggg
    17401 cgctgtgcgc ggcgccacgc ggcgccgggc gaggtgtaga gcacggccag gccggcggtc
    17461 aggcccgcgc agtacagggt ggaccaggtg aacgggtacc aggttcgcgc gagccgtagg
    17521 gccaatggca gccggggcag ggtcaggagc gcggcggtgg ctgcggtcac gccactgacg
    17581 gcgaggaacg gctcgctcag tgtgccgatc gcgaagcctg tcaccagggc gccgacgagc
    17641 gccgcctgtc gtggccgtcg cctcgggctc cgggccgcgc ggatggcggc gagcagggcc
    17701 cagatgccca ggacgctggg caggatgtgc gagatggcgg ccggggccca cagcatctgg
    17761 taagtgcggg taccgccgag gaacagcagc gcctccacca ccagcaccgc gggcgccagg
    17821 actgccagag gtgcccgcac gtccagcgcg ccgagcattt cacgggccag caggtacagg
    17881 ccgagcccga gggcgatgat cagaaccgtg gagagcacct gcgggccgag catgccgtgg
    17941 gagtagacga ccccgctgag gaaggcgttg gtgatccgcc cgttctgtgt caggtagaag
    18001 tcggacgtca cacccaggac gcccatgtcc cgggtcttcc acgccatgca ccagtcatcg
    18061 gaggtgggcc gcaggaaggt ccccaggaag ccggcgatgc ccagaagcgc gccggccgct
    18121 gccgtcagaa ccccaccggt tccgcgcaac agcccggcgc cggcgctctt gcccggcggc
    18181 ccggcagggc cgggtccggt gccggcggcg ctggtttggc cggccggccg cgcgatcggc
    18241 ttccttgcct gcacgggtag taccgcccgg tcaggcaggt cccgtgtcgg gctccgttcg
    18301 gcacggtcct gcgcgtcaga ggttgtcacg ggttgtgaac gagttacggc cgaccagggg
    18361 aacggtccgg tgatgtgccg tggtcgttcg catgactttc gctcgatccg tttccacaca
    18421 ccgtacggct cacgcgcgcc gcagggcggc gacgagggcc cgcaagcggg accaggccca
    18481 ggtggtagag gtgtgcctcg ccgagccccg cgggcagggt ctcgggacgg aagtagccgc
    18541 cgacggcggt ggcgggtggc gcaagatcgc ggagggcggc aaccggacct ccgccggacg
    18601 cgggcccggg ctcgggcctg gacccggacc cggaacctga gtcggccgcc cagcagttgg
    18661 cgaccaccgc gtcgatgccc tcggcgaccc tcggccccag gggcgcgaag gccccggtgg
    18721 cccaggggga cgcggagccg tgcacggtga tgcggatgcc gggcgcggcg gtacgcgcgg
    18781 cggcgatcag gcgcgcgtgc agagcggatg cggacgcgtc ggcacactcg cgcagcacgg
    18841 cggccgtctg tgccccgacg gcgtcctcgg ggctcttcgg cggctgcgcc ggcccgaggg
    18901 ccgtacggac ttgagccgcg accgcggcaa gctgcgcgtc accctccacc acaatctctt
    18961 cgagaacgtc gtcgagaggg cgccacgggt ccgcttcggc aaggtcgacg cgtacaacaa
    19021 tcacttcgtg gtgcccggtt cggcacactc ctacagcttg agcatcggaa tcgagtccca
    19081 gctcgtcgcg gagaagaacg ccttcacgct cgccgatggc gtccaggccg gagagatcct
    19141 caagaagtgg aaggacgccc cggtcaccac tgcgggcaac ttcgtcaacg gcaggcaggt
    19201 cgacctcctc gcggcccaca acgcccagtt ccccggcgag atcctgcgcg ccgacgcggg
    19261 gtggacgccg acgctgcgca cgcgggtcga cgtgccgcag gccgtgcccg ccctcgtcga
    19321 ccaccgcgcg ggcgcgggcc gactgcactg accttccgcc cgccgctctc gcccacttcc
    19381 gcagcgaaga cgctccccct gccctcgcac cacccacgag cctcagcagt gcagaaagaa
    19441 gcattccgct gcacacgcct atcgcgaggg cgtcgatgac tgctagaaga gtgacgcttc
    19501 gacaactcct gatccccaca aagccgcaag gagttctttg tcatgcacat gtacaaattg
    19561 agatgggtcg tgccctcgtt actgaccctg gtcgccgcga ccctcgggct cacccacccc
    19621 gcctccgccg ccacggcagc aggggagcgc tacgtcgctc tcggtgactc ctactcctca
    19681 ggggtgggcg ccggaagcta cctgtccgac agcggcagct gccagcgcag cagcaaggcc
    19741 tacccgtccc tgtgggcagc agccaacgcc ccggcctcct tctccttcac cgcctgctcg
    19801 ggagcgacga ccagcaccgt caagagcagc cagctgacgc cgctcaacag ctccaccacg
    19861 ctcgtcagcg tcaccgccgg cggcaacgac gtcggattcg cgaacgtcat gcagacctgc
    19921 gtcctgcaga gcgagtcgac ctgcgtcaac gcggtgaaca acgccatcgc acaggtcaac
    19981 aacaccctcc ccggcagcct ttcgtccctc tacggcacca tccgcagcaa ggcgccgcag
    20041 gctcatgtcg tcgtgctcgg ctatccgcgc ttctaccagc tgaacggcag ttgcatcgcc
    20101 ggcctgagcg aaaccgagcg ggctgcgatc aaccggggca gcgacgccct caacggcatg
    20161 ctcgcgaagc aggccgccaa cgcgggattc tcctactcca gcgtcgtgga cgagttcacc
    20221 ggccacgaga tctgctcggg cgacgcctgg ctgcacagcg tctcggtgcc cgtctccaac
    20281 agctaccacc ccacggccac gggccacgcc agcggatacc tgccggcctt ccgctccgcc
    20341 gtctgatccc cccaggctcc ccagactgca tccccggggt cttccgcgcc cgagggtcga
    20401 cgcgggtaga cccagcggct ctccgctcag tgcctccgca cccatccgcc gaatccgccg
    20461 aatccgcacc aggaggaaag ctcatgacgt catcccaggc cggcgacacg gccacaagca
    20521 gcacacgacg aaacagactc cgctccgtcc tcaccgccct cgtcctcgtc ctcggcgcgc
    20581 tcttcgcccc cggcgtcggc gtcctcggcg gcgccggtga ggcccacgcg gccaccaaga
    20641 tcagccacgc caccgcgacc tcgatgttcc gcaactccgc catcacctgg tcctcgtccg
    20701 gcggctgctc ggaccgcaac aacccgacct gcacgtcctt cgagcagctc aacctcgcca
    20761 ccgcgcaagg cgcccagacc ctcaagagcg ccagcggctg cgccctgaac atcaccggcg
    20821 gcaccgagac cggccacgcc ggcggcacc
//
