LOCUS       NZ_CP102509            10870 bp    DNA     linear   CON 06-JAN-2024
DEFINITION  Streptomyces sp. NBC_00162 chromosome, complete genome.
ACCESSION   NZ_CP102509
VERSION     NZ_CP102509.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. NBC_00162
  ORGANISM  Streptomyces sp. NBC_00162
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8635149)
  AUTHORS   Nielsen,J.B., Gren,T., Mohite,O.S., Joergensen,T.S., Klitgaard,A.,
            Mourched,A.-S., Blin,K., Larsen,T.O., Weber,T., Gotfredsen,C.H. and
            Charusanti,P.
  TITLE     Identification of the biosynthetic gene cluster for pyracrimycin A
            and elucidation of a key reaction during its biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635149)
  AUTHORS   Joergensen,T.S., Gren,T., Blin,K. and Weber,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2022) Novo Nordisk Foundation Center for
            Biosustainability, Technical University of Denmark, Kemitorvet,
            Building 220, Kongens Lyngby, Hovedstaden 2800, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-AUG-2022
            Assembly Method :: Flye v. 2.9-b1768; Polypolish v. 0.5.0; POLCA
            polisher from MaSuRCA v. 4.0.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina Nextseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 18:03:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,275
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,805
            CDSs (with protein)               :: 7,805
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 371
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 371
            Pseudo Genes (frameshifted)       :: 186 of 371
            Pseudo Genes (incomplete)         :: 231 of 371
            Pseudo Genes (internal stop)      :: 27 of 371
            Pseudo Genes (multiple problems)  :: 68 of 371
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:41:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 17969
            Orig. end                         :: 28839
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102509.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Tilmann Weber, Dr.
            Professor, Novo Nordisk Foundation Center for Biosustainability,
            Kemitorvet, 220, 327D 2800 Kgs. Lyngby, Denmark.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5870](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5870)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1131..2066)
                     /locus_tag="JIW86_RS00110"
                     /old_locus_tag="JIW86_00110"
     CDS             complement(1131..2066)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /locus_tag="JIW86_RS00110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_00110"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_257551922.1"
                     /transl_table=11
                     /translation="MGAAPARCAPRLEAAAEVRDLAEEFALDEQILSAVLDYVCRTTGL
                     LIREDRCYRLAKACRPYHRVAFHLEKFLGAYGPAVARTDEVLQDPGTGPALRDLNALAR
                     AFGQLQPHTPSLTAQVLQAWDVTSLLDLGCGAGTLLIELASADPRFQGYGADAGQDMVR
                     VANDRIHRAGLGHRLQVVLGDVRALGELTALPASEDVQALHGRSIMNEFFAGDGSSAIG
                     VLAGLKARFPGRLLFLEDYYGRLTHDSPADGSHGHTLAQDLAQVLSGQGVPPLDLPGWA
                     GVYQEADCTLVKAYEGDNDGVAWFIHVVAL"
     gene            complement(2124..2468)
                     /locus_tag="JIW86_RS00115"
                     /old_locus_tag="JIW86_00115"
     CDS             complement(2124..2468)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS00115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_00115"
                     /product="hypothetical protein"
                     /protein_id="WP_257551923.1"
                     /transl_table=11
                     /translation="MLRDIHACPEILDVLSAAPAPEAAEIPAGLRTQKGQYLLLPGDSE
                     HTVRNLTISGDSVRLLTFLEAPRSKSEIAQYIGVADSNAESLGALLGRLLDLGVLQHRE
                     LREPQPAAGA"
     gene            complement(2423..4117)
                     /locus_tag="JIW86_RS00120"
                     /old_locus_tag="JIW86_00120"
     CDS             complement(2423..4117)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="JIW86_RS00120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_00120"
                     /product="radical SAM protein"
                     /protein_id="WP_257551924.1"
                     /sec_met_domain="PF04055 (E-value: 4.2e-17, bitscore: 61.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVTFIDNLLLEQGADGYHFDLQPHLGLISLLAVLRQGGHQGTLI
                     DPKLEVSSGRLLLDASLYENIAELVLASQPDVVGMTSLGCNFMATAKIAAHLKRRSPDL
                     PVLLGGPHASILDVAVLQRFPQFDLVVRGEAELTLLPLLDSLHSRQFSGLLGITHRSAQ
                     GVHRSPDAPLVADLDTLAPAAYDHYPVAQAGQELLRVEAGRGCPFACTFCSTASFFGRR
                     YRLKSAERLCSDLDRLHETYGISHFALTHDLFTVNKTKVREFCEAVAPRGYTWTCSARM
                     DCVDSELLDAMARAGCRSIYYGVETGSPRMQKEVAKRLDLALFWPTLEATSRTGMESIA
                     SFITGYPQETAEDQQMTLDLIGEAWIRHADMLTTQLHLLTPEPGTELLDQYRDQLAYDG
                     HISDFNLPALEADDADITRNDPEIFVNHHYYRGVLPRQRHVLVTSVYRALCRLNRNVQR
                     HLLARYNNSLGGFFDDLLSWAGTDADAPSDAPELLQTYLAGRWGAEDYLLSLVRYLLAA
                     SDPVRGASGGAAGLPEPRSHSTHPLLQLSPVPPCSGTSMPVRRSWTC"
     gene            complement(4120..5016)
                     /locus_tag="JIW86_RS00125"
                     /old_locus_tag="JIW86_00125"
     CDS             complement(4120..5016)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS00125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_00125"
                     /product="hypothetical protein"
                     /protein_id="WP_257551925.1"
                     /transl_table=11
                     /translation="MPDLVNSDINSFVARCLLDPGYLDRLARNPHDEIAALPVSTGTRT
                     AIAGLDFERVRLFAGFISKVQHNDLWDDFPCTRSLLRYYRVELATFADYRAHHLELARA
                     GKPTRAAKTEAFLDFLESRLRRPVGPEYPGLLDVLTHERLHWEVTRSLLERPGPDQGGS
                     PVRGRPAPGPVRADRPDRRTVVACDVLRVAALSYDPLDVTARLHDGGRALADLAPNAVC
                     LCYWGRESAGTVSVLAVDPPTASVLAAVDNYRPVHQVLDEGQTLLPEADRGDLARVLDK
                     AVGQGLVRLIDIPAAVE"
     gene            complement(5001..5870)
                     /locus_tag="JIW86_RS00130"
                     /old_locus_tag="JIW86_00130"
     CDS             complement(5001..5870)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="JIW86_RS00130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_00130"
                     /product="DUF692 family protein"
                     /protein_id="WP_257551926.1"
                     /sec_met_domain="DUF692 (E-value: 3.3e-31, bitscore: 107.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGFLTSTRPEVGVAYSRSTPDFIRRNPGSVDYIEVPFELLLHDP
                     TVATRVGSTPVVLHCASLSIAGSVAPEAAVISSVEDWIGRTGTPWLGEHLSFITAEREK
                     PGRFADEYAPGEPWNIGYTVSPPMNSATVESVVRSAELAASAFSVPLILENPPVYFSMP
                     GSTMSQVEFISEVCARSEARLLLDLAHFYITSRTLGCNPMTELARLPLEKVVEVHVSGV
                     DTDAGGCWDHHASRAPQLELDLLELVIERAPVCAVTLEYNWSSRFPESVLLEEIARVRD
                     VIGACRTW"
     gene            complement(5928..6122)
                     /locus_tag="JIW86_RS00135"
                     /old_locus_tag="JIW86_00135"
     CDS             complement(5928..6122)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS00135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_00135"
                     /product="hypothetical protein"
                     /protein_id="WP_257551927.1"
                     /transl_table=11
                     /translation="MKHTEGPQVIVTPSSLLFTLTPEHREKARSCLETSGEIRISFEEV
                     SVTNLTEIRELGREGVIID"
     gene            <6313..>6672
                     /locus_tag="JIW86_RS00140"
                     /old_locus_tag="JIW86_00140"
                     /pseudo=""
     CDS             6313..>6672
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460478.1"
                     /locus_tag="JIW86_RS00140"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="JIW86_00140"
                     /product="ISAzo13 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGRIRAAGAGCERLRDTDPESPLRWTTKSTRKLAEELSGAGHRVG
                     ADSVAALLKAEGFSLQATSRTTEGARHPAGTRSSAYINERVAEHLDAGQPVVSVDAEKK
                     EVLGEYAVAGREWHRS"
     gene            complement(6961..7605)
                     /locus_tag="JIW86_RS00145"
                     /old_locus_tag="JIW86_00145"
     CDS             complement(6961..7605)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS00145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_00145"
                     /product="hypothetical protein"
                     /protein_id="WP_257551928.1"
                     /transl_table=11
                     /translation="MTSPTANVQNTPELSQEFQEMWVRRGEPFKRKNPFTPTLPVIIEG
                     EVVYDLERMNLEYPGELYVTPVVHRSTLALATFVDAKEMLRKAQEVTGDDELRQLLRAS
                     ALGCVADGDPVRLVLDCYDRQNLEGSKLRLDPEAMCPELYKLKRGNNFPPLNWDNAIRS
                     VDACAYPYSVSSDPWFHGTTHFINFRCSFNATTFGYTASSIRNWGTLPPEF"
     misc_feature    7788..7804
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     misc_feature    complement(8238..8253)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.12"
                     /tool="antismash"
     gene            <8573..8879
                     /locus_tag="JIW86_RS00150"
                     /old_locus_tag="JIW86_00150"
                     /pseudo=""
     CDS             <8573..8879
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377440.1"
                     /locus_tag="JIW86_RS00150"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="JIW86_00150"
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PAGRLLWISPALPPHTRSHCSPSRRPRLRGSRLVGDNTPQAPAGP
                     ELTPTRQPANRALSAARTPVERVARPKSWLIFCRSRCSESNVVHRSDDRHPGAATPI"
     misc_feature    9282..9296
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: strong, score: 20.76"
                     /tool="antismash"
     misc_feature    complement(9383..9397)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            9793..10299
                     /locus_tag="JIW86_RS00155"
                     /old_locus_tag="JIW86_00155"
     CDS             9793..10299
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JIW86_RS00155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JIW86_00155"
                     /product="hypothetical protein"
                     /protein_id="WP_257551929.1"
                     /transl_table=11
                     /translation="MSEGGNVLDGQRSGPEQVGPAPWLALACLVGLILAYVVLTNFTAE
                     LRAALWGEPGTVTEADCRTYDNTVANAGVSTSCSGAFTPDDGGSSFDVPVEGDIDSPPV
                     RAWLVDGSSGTAYVSPTVWAGVFPVGLSLLLAGLPAAVTVKHLRRVNAQRRREPLHGSD
                     TESSA"
     misc_feature    complement(10537..10552)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.6"
                     /tool="antismash"
     gene            10573..10854
                     /locus_tag="JIW86_RS00160"
                     /old_locus_tag="JIW86_00160"
     CDS             10573..10854
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158482.1"
                     /locus_tag="JIW86_RS00160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JIW86_00160"
                     /product="hypothetical protein"
                     /protein_id="WP_257551906.1"
                     /transl_table=11
                     /translation="MKSFPFPDDLIKLQQQWTAAYQRLADRPGQGGAELRRELIVLSCR
                     ISAHPYWEGSGWSTAGRVELRQAAEAADRAAGHRRGGFAAAGPGARSR"
ORIGIN
        1 gcagatctac ggggtcgagg tcagtcccga tctgatcagc aaggtcaccg atgccgtgat
       61 cgacgagctc gtgacctggc agaaccggcc cctggatgcg gtctggccga tcgtctacat
      121 cgacgctttg tgggtgaaga tccggtccgg ttcagtgacc tcgaagcccg tctatctggc
      181 cgtcggcgtg gacatggacg gccgcaagga cgtcctcggt ctgtgggtcg gagccgaggg
      241 cgaaggcgcc accacctgga tggcagtcct gtcggagctg cggaaccggg gcatcgagga
      301 tgtctgcatc gtggtctgtg acgggttgaa gggcctgcct gacgcggtca ccgcgacctg
      361 gcccaaggcc accgtccaga cgtgcgtgat ccacctgacg agagcctcgc tcaggctgtc
      421 gtcgtcgcgg gaccacccga agctggtgcc ggccctgaag gcgatctaca cggcaccgac
      481 cgagcaggcc gccgagcagg ccctcgatct gttcgcagcc tctgacctgg gcgagcgcta
      541 cccggccatc gtgcggacgt ggcgatctgc ctggccggag ttcacgccct acctggcgtt
      601 cccgccggcc ataaggacgg tggtctactc cacgaacatg gtcgaatcca tgaacgcccg
      661 cctccgcaaa gccacccgca accgcggcca cttcccctcg gaacaagccg cgttgaaggt
      721 cctctacctc gcggtccgcg aacagatcaa ccccaaagcg cgcgacgcga accacgtcgc
      781 cgcacactgg aaagaggcac tgaaccagtt ctcactcttc ttcgaggacc ggctcagcat
      841 ccaatgacac caaccgactt acacaaaatc gctgacacgc ccacctggcc agcacgactc
      901 ctctggagga cctcgcaacg gcctggacaa ccgcggagcg cgtacgggaa tgggccttgg
      961 acctgtgcac gcgcgtcgag gaggaactag ctgccgggcg ccggaggcgc acgccacgtg
     1021 tacctccgcg atcatgcacc gatggcaaac ctgctggtca caggattttg atcaacccgc
     1081 agaagatcta caccccgggc ggatcgcccc ctgaaccggc cacccggtcc tcagagggcc
     1141 acgacgtgaa tgaaccacgc gactccgtcg ttgtccccct cgtatgcctt caccagggtg
     1201 cagtcggctt cttgatagac cccggcccag ccgggcaggt ccaggggcgg gacgccctgc
     1261 ccactgagca cctgcgcgag atcctgcgcc agggtgtgcc cgtgagaccc gtctgcgggc
     1321 gagtcgtggg tgagccggcc gtagtagtcc tccaggaaga gcagacggcc cgggaaccgc
     1381 gctttgagcc cggccagaac gccgatggcg ctgctgccgt cgccggcgaa gaactcgttc
     1441 atgatgcttc tcccgtggag ggcctggacg tcctccgatg ccggcagcgc ggtcagctcc
     1501 ccgagcgccc gcacatcgcc gagcaccacc tggaggcggt ggccgaggcc agcacggtgg
     1561 atgcggtcgt tggcgacccg caccatgtcc tgaccggcgt cggcgccgta gccctggaac
     1621 ctggggtccg ccgaggcgag ttcgatgagc agggtgccgg cgccgcagcc cagatccagc
     1681 agcgaggtga cgtcccatgc ctgcagcacc tgagcggtca gcgacggggt atgtggctgc
     1741 agttgcccga acgcccgggc cagagcgttg aggtctctca gcgcagggcc tgttcccggg
     1801 tcctggagga cttcgtcggt ccgggcgacg gcaggtccgt aggcgcccag gaacttttcc
     1861 agatggaacg cgacgcggtg atagggacgg caggccttag cgaggcggta gcagcggtcc
     1921 tcccggatca gcaggccggt cgtccggcaa acgtagtcca gcaccgcgga gaggatctgc
     1981 tcatccaggg cgaactcttc ggcgaggtcg cggacttcgg ctgctgcttc gagccgggga
     2041 gcacaccgag ccggtgcagc gcccacacgg actgcgaggc gtagaaccct tccaccacgc
     2101 ccatgacgtt caatagcggc atgtcaggct cccgctgccg gttgaggctc cctgagctcc
     2161 ctgtgctgga gaacgccgag gtcgagcaga cggccgagga gcgcgccgag cgattccgcg
     2221 ttactgtccg cgacgccgat gtactgcgcg atttcggatt tgctgcgcgg cgcctccagg
     2281 aaggtgagca accgtaccga gtcgccactg atggtgaggt tgcgcaccgt gtgctcactg
     2341 tcgcccggca gcagcaggta ctggcccttc tgcgtgcgca gcccggctgg gatctccgcg
     2401 gcttccggcg caggggccgc gctcagcacg tccaggatct ccggacaggc atggatgtcc
     2461 ctgagcacgg cggcacgggg gatagctgca gcagcggatg agtggagtgc gaacgtggct
     2521 ccggcaggcc cgccgcgccg ccgctggccc cacgcacggg gtcggaagcg gcgagcagat
     2581 agcggaccag ggacaggagg tagtcctcgg cgccccatct gccagccagg tacgtctgca
     2641 gcaactccgg ggcgtccgag ggggcgtccg cgtcggtgcc cgcccacgag agcaggtcgt
     2701 cgaagaaccc accgagactg ttgttgtacc gcgcaagcag atgacgctgc acattgcggt
     2761 tgagccggca cagggcccgg tacacggagg tgacaagaac gtgccgctgc ctgggcagga
     2821 cacctcggta gtagtggtgg ttgacgaaga tctcgggatc gttacgggtg atgtccgcgt
     2881 cgtccgcctc gagagcgggg aggttgaagt cggaaatatg accgtcgtat gcgagctggt
     2941 cccggtactg gtcgagcaac tcggtgccgg gttccggtgt cagcaggtgc agctgtgtcg
     3001 tcagcatgtc ggcgtgccgg atccaggcct caccgatgag gtccaaggtc atctgctgat
     3061 cctcggcggt ctcctggggg tagccggtga tgaaggaggc gatcgattcc atcccggtcc
     3121 ggctggtggc ttcaagcgtc ggccagaaca gggcgaggtc gaggcgcttg gccacctcct
     3181 tctgcatgcg cggtgacccg gtctcgactc cgtagtagat ggagcggcag ccggctcggg
     3241 ccatggcatc caggagttcg ctgtcgacgc agtccatgcg cgcggaacag gtccaggtgt
     3301 agccccgtgg cgccaccgct tcgcagaact ccctaacctt ggtcttgttg accgtgaaca
     3361 ggtcatgggt cagtgcgaag tggctgatcc cataggtctc gtgcagacgg tccaggtcgg
     3421 agcagagccg ctccgcggac ttcagccggt accggcggcc gaagaaactg gcggtcgagc
     3481 agaaggtgca ggcgaagggg cagccgcgac cggcctccac ccggaggagc tcctgcccgg
     3541 cctgcgccac gggatagtgg tcgtaggccg ctggcgcaag cgtgtccagg tcggcgacca
     3601 gcggcgcatc ggggctccgg tgaacaccct gagcactgcg gtgggtgatg ccgagcagcc
     3661 cggagaactg ccgggaatgc agtgagtcga gcagcggcag cagggtcagt tccgcctcgc
     3721 ctcgtacgac gagatcgaac tgcgggaagc gttgcagcac ggcgacgtcg aggatgctgg
     3781 cgtgcggacc gcccagcagg accggcagat cggggctgcg gcgcttcagg tgtgccgcga
     3841 tcttggcggt cgccatgaag ttgcagccaa ggctcgtcat gccgaccaca tccggctgtg
     3901 aggccaagac cagttcggcg atgttctcgt acagcgatgc gtccaggagc agtcggcccg
     3961 agctgacctc cagcttgggg tcgatcaggg tcccctggtg gcccccctgc cggagaacgg
     4021 cgagcaggga gatgaggccg agatgcggct ggagatcgaa atggtacccg tccgccccct
     4081 gttcgaggag aaggttgtcg atgaaggtga cccgcatatt tactccactg ccgcagggat
     4141 gtcgatcagc cgtaccagcc cctgccccac cgctttgtca agtacccggg ccagatcgcc
     4201 gcggtcggcc tccggcagca gggtctggcc ctcgtcgagc acctgatgga ccggccggta
     4261 gttgtcgacg gcggccagga cggaggcggt gggcgggtcc accgccagaa cagacaccgt
     4321 tcccgcgctt tcgcgtcccc aatagcagag acagacggca ttcggtgcga ggtctgccag
     4381 cgcccgtccg ccgtcatgga ggcgggccgt gacgtccagt gggtcgtagc tcagcgcggc
     4441 gacgcgcagc acgtcgcatg cgactaccgt gcgacggtcg ggacgatcgg cgcggacagg
     4501 ccccggggcc ggcctgcccc ggaccggact gccgccctgg tccggaccgg ggcgttcaag
     4561 gaggctccgc gtcacttccc agtgcaggcg ctcatgggtg agcacatcca gaagcccggg
     4621 atactcgggc ccgaccgggc ggcggagacg actctccagg aaatccagga aggcctcggt
     4681 cttcgccgca cgcgtcggct tgcccgcgcg tgccagctcc agatggtggg cacggtagtc
     4741 cgcgaaggtg gcgagctcga cccggtagta cctgagcagg gaacgggtgc agggaaagtc
     4801 gtcccacaga tcgttgtgct gaaccttgct gatgaacccg gcgaacagcc gtacccgctc
     4861 gaagtcgagc ccggcgatcg cggtccgggt cccggtactg accgggagcg ccgcgatttc
     4921 gtcgtgcgga ttccgggcca gccggtccag atatcccggg tcgagcaggc atcgggccac
     4981 gaatgagttg atgtccgagt tcaccaggtc cggcatgcgc cgatcacatc ccggactcgg
     5041 gcaatctcct ccagcaggac actctcggga aaacgggagg accagttgta ctccagcgtc
     5101 acagcgcaca cgggggcgcg ctctatgacc agttcgagca ggtccagttc caattgcggc
     5161 gcacggctcg cgtggtggtc ccagcacccg ccagcgtcgg tatcgacgcc cgagacgtgc
     5221 acttccacca ccttctccag gggaaggcga gcgagctcgg tcatcggatt gcatcccagg
     5281 gtccgggagg tgatgtagaa atgtgcgaga tcgagaagca gacgggcctc cgaccgtgca
     5341 cacacctccg agatgaattc gacctggctc atggtggagc cgggcataga gaagtacaca
     5401 ggcgggttct caaggatcaa cgggacgctg aaagcggacg cggcaagctc cgccgaccgg
     5461 acgacggact cgacagtcgc ggagttcatc gggggactga ccgtataccc gatgttccag
     5521 ggctcgcccg gtgcgtactc gtcggcgaac cggccaggct tctccctctc ggccgtgatg
     5581 aacgacagat gctctcctag ccacggggtc cccgtccgcc cgatccagtc ctccacgctg
     5641 ctgatgaccg ctgcttccgg tgccaccgac ccggcgatgg aaaggcttgc gcagtgcaga
     5701 acgacgggtg tcgaaccaac tcttgtcgcg accgtcggat cgtgaaggag gagttcgaag
     5761 ggaacctcga tatagtccac cgagccggga tttcgccgga tgaaatctgg ggtagacctg
     5821 ctgtacgcga ccccgacctc aggcctggtc gatgtcagaa atcccggcac cttttcgtcc
     5881 gtttcccttt tatgtcggtc tcagtggaga ggcgagtccg atgggattca gtcgatgatt
     5941 acaccttccc tgccgagttc ccggatctcg gtcagattgg tcacggaaac ctcttcgaac
     6001 gagatccgga tctctccgga cgtctcgagg cagctgcgcg ccttttctcg atgttcgggt
     6061 gtgagcgtga agagcaggga tgacggtgtg acgatgacct gagggccttc ggtgtgtttc
     6121 ataacttcat tctctgccgg tctcctgacc ggatgtcaag tgagagtcgg cagaatgccc
     6181 cgtacggccc atacggcatt ttcgtaggat ggccatgcgg tatgtcgagc ggcgcgtcaa
     6241 ctaccgcccc gcgcgggcat cgatgtacag gcagtgcggt gagcagcgca agctggacgg
     6301 cgggcggtgc gcagcggtcg catccgggcg gcgggagcgg gctgcgagcg gttgcgggac
     6361 accgacccgg agtccccgct gcgctggaca accaagtcca cgcgcaagtt ggccgaggag
     6421 ctgagcggag ccgggcaccg ggtaggcgcc gactcggtcg ccgccctgct caaggctgag
     6481 ggattctcct tgcaggccac ctcccgcacc accgagggcg ctcgccaccc ggccgggacg
     6541 cgcagctccg cctacatcaa cgagcgggtc gccgagcatc tggacgccgg gcaaccagtg
     6601 gtcagcgtgg acgccgagaa gaaggaggtg ctcggagagt acgccgtggc ggggcgcgaa
     6661 tggcatcgct ccgaacgccg tctcgtacgg cgtctcacag ggctgattca aagtcggggt
     6721 gatcatgtgg cgtcgctggt cacgggcgac cggtgatctc ctggaggagc gtcacgtacg
     6781 caacgaagct ccggtttggg ggcatcgagc ggagtcacac atcccggcca gcggacggct
     6841 gcgatcttcg gcctgggcga gtttgaggca gagggccttg tccgggtcga cgaggcccga
     6901 ggctgacatc tgaaccccgc ccaagggcgc gccctgcgcg ggaccgcctg ctcccgctac
     6961 tcagaattcg gggggcagtg ttccccaatt cctgatcgac gaagcggtgt agccgaatgt
     7021 ggtcgcattg aacgagcagc ggaagttgat gaagtgggtg gtgccatgga accatggatc
     7081 actcgacacg gagtacgggt aggcgcatgc gtcgacagag cgaatggcat tgtcccaatt
     7141 aaggggaggg aagttgttgc cgcgcttgag cttgtacagt tcagggcaca tggcttccgg
     7201 gtccagcctc agtttgcttc cctcaagatt ctgcctgtcg tagcagtcga gaactagccg
     7261 cactgggtcg ccatcggcga cacagccgag ggcgcttgcc cgaagcaact gacgcagttc
     7321 gtcatcgccc gtcacttctt gggctttcct cagcatctcc ttggcatcga cgaaggtagc
     7381 caaagcgagc gtcgatctgt gcaccacggg cgtaacgtac agttctcctg gatattccaa
     7441 attcatgcgc tcaaggtcgt agactacctc tccctcgata atcactggca gggtcggggt
     7501 aaacggattc ttccgcttaa acggttcgcc gcgccgaacc cacatttcct ggaattcctg
     7561 cgacagttcc ggagtgttct ggacattggc agtaggggat gtcattgcaa cctccgatag
     7621 gagaagttgt agaaatctca gagagttcga ggggtgatac gcaaacagca cggagttctg
     7681 ttccgtttca ccgcagtgga gcgggagcta ccgtcagccg gccccgttcg cctgatggtc
     7741 actcggtcaa catcggagag cgcccagact ccgtcctgca gcgggatctg cgcatgactg
     7801 gtaaggacac ccacgggaca cccgccagca caccctttat ggagccacta tccacggcct
     7861 aggtgatagc ccttagggat gcgctttgcc gaccgtcgat gcgatccagg agaccgggtg
     7921 tcgaacgcca tcagccggtg tgcatccacc gcctcaccgg gacaaaggcg ccgaagccgc
     7981 caacatcttc cgttcacctt ccagttcaca cctggccggc cgtgatcgca ttctcgactg
     8041 atgccagagg tctctccgat agcggcaagc cgagcagccg ctcagcgcca ggccacctgc
     8101 tccgcaaaat ggtgaacgtc ggatcgagcc cttcgatctc gcctcgccag tcctcttcga
     8161 cacctcgttt gcggcgagcg aggaggtcct cctccagctc gttgaggcga gacagcacct
     8221 tcgggttgac gctgaggaat cgtcaagacc tgtggatcaa gtcagcttgg ggctgttgtg
     8281 gtcgttgggt tcggcggctc gccgcgttgc ggacacggcg gtcgcagtcg ccccccgctg
     8341 tcacctgtgc ccagacgagg accaggtcct cgccaacccg gccctcacgg ttaccttcat
     8401 tcgccttcag aatcgccatc cggccgcacc gcccagtcca ccggcgtcgt cgtcgacctg
     8461 atggaggccc tgcgccagcg ccgaacgggc aagcccgagt gactggcgag aaggccagcg
     8521 cctccagcac gagcgctgcc aggaggaagc ctcgcgcgaa gaagcgcgcc accccgctgg
     8581 acgtctgctg tggatctcac ccgcactgcc cccgcacaca cgatctcact gcagcccaag
     8641 ccgacgaccg cgcctacgtg gaagccggct cgtgggtgac aacacccctc aggcgcccgc
     8701 tgggcccgag ctcacgccga cccggcaacc cgccaaccgt gcgctgtccg ccgcacggac
     8761 accggtcgag cgcgtcgcgc gaccgaagtc gtggctgatc ttctgcaggt cccggtgcag
     8821 cgaatcaaat gtggtccatc gcagcgacga tcgtcaccct ggagcggcaa cgccaataag
     8881 gggccctgaa ggttttcata cgagtcccgt gcattctact tcgaagtcag tgccggtccc
     8941 ccagggaata cacaacagat gactgtacct tcgggtgtgc ctacgacttg gcagtcagct
     9001 cgtaggctca ctttatcttc tttggatttc cgggccttag tggacttgcg aatgctggat
     9061 gcggcgctag acaaggaggc ttgaagctct gagcacaagg ttagaaggaa tcccagatcc
     9121 tgagggacct cctcggggcg cttgaattta accatgacga gagggtgctc atggtcttgg
     9181 catttaaggt tcttcaagtg cgtgaacgac gccaccaaat cagtggggag cgtcagccac
     9241 ctttcgcatg tgacacgttg cgtgaaccta attaccgatg aatcactttc gtcgatcttc
     9301 accatgccga ctacgctgag ttctgtaggg tccggcaact cgttgtttga gagcctggaa
     9361 agaaactctc tcggttcgtg agttcgcatc ttgagcacct ccacaactcg gcttaaatcc
     9421 gcagcacgga aggcgttgca gccaacacgt cacttgggat tttccaagct tagttcggtc
     9481 acaccgcagg ggcatcccaa ctccccctcg ctctctcacc gccgtcgctg gcagcaacac
     9541 cgacgcagaa gtactgcttc ttcttgcccg agacgaacgc gcggcgtccg ccgcggtcgg
     9601 ccacgggcct gcggaccgct tcagttggcg ggattcgcca tgtacgacgc tcacccggag
     9661 gcggatccga cgcggccgac gacatccact ggccggtgtc ggtcgtgcga gcccaccagt
     9721 acgtcgtgga gggcttccga gggcacctcg gatcggtcac catgaaggtc cgtcggagat
     9781 ccgagggtgg gaatgagcga gggtgggaac gttctggacg ggcaacggag cgggccggag
     9841 caggtcggac ccgccccgtg gctggccctg gcctgtctgg tcgggctgat tctggcctac
     9901 gtcgtactga cgaacttcac cgcagagctg cgggcggcgc tgtggggcga gcccggcacc
     9961 gtgaccgaag cagactgccg gacgtacgac aacactgtgg ccaatgcggg agtgtcgacg
    10021 tcctgctcgg gcgccttcac cccggacgac ggcggctcgt cgttcgatgt ccccgtcgag
    10081 ggcgacatcg attcgccacc ggtccgcgcg tggctggtgg acggttcgtc cggcaccgcg
    10141 tacgtctcac caacggtgtg ggcgggggtg ttcccggtgg ggttgtccct cctcctggcc
    10201 ggtctcccgg cggcggtgac ggtgaagcac ctgcgccgcg tcaacgcaca acgccgtaga
    10261 gagccgcttc acggatcaga cacagagtcg agtgcgtgaa cgacgacgag cacagcgggt
    10321 acagcgcgca cgtcacctgg ctcgacatcg acaagccggg cctctggcca ggggcgcggc
    10381 ccgcgaccga gggcccggct cccgccgtgc gctccgcagc gccgccggcc tgccatggag
    10441 tcgtccgcca tctacgctgc tcagctccgc caaggagcgc gctcacacgc caactgaagc
    10501 gctcagtcac aggcgccacg agcacctcgt cgggactcgc acaataattc gaacacctga
    10561 ttgaataagt ggatgaagtc cttcccgttc cccgacgacc tgatcaagct ccagcagcag
    10621 tggaccgccg cctaccagcg cctcgccgac cggcccgggc aaggaggagc ggagctccga
    10681 cgggagctga tcgtgctgtc gtgccggatc agcgcgcacc cctactggga agggtctgga
    10741 tggtccacgg cgggccgggt cgagcttcgt caggcagccg aggcggcaga ccgcgcggcg
    10801 ggacaccgcc gtggtgggtt cgcggcggcc ggcccagggg cgcggtcgcg gtgagcaggg
    10861 tccaggcgcg
//
