LOCUS       NZ_CP102332            11449 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces changanensis strain HL-66 chromosome, complete genome.
ACCESSION   NZ_CP102332
VERSION     NZ_CP102332.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces changanensis
  ORGANISM  Streptomyces changanensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Streptomyces changanensis sp. nov., an actinomycete isolated from
            soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) College of Forestry (Academy of Forestry),
            Northwest A&F University, Northwest A&F University, Yangling,
            Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 187.45x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024600715.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 19:18:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,071
            CDSs (total)                      :: 5,982
            Genes (coding)                    :: 5,812
            CDSs (with protein)               :: 5,812
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 70 of 170
            Pseudo Genes (incomplete)         :: 131 of 170
            Pseudo Genes (internal stop)      :: 9 of 170
            Pseudo Genes (multiple problems)  :: 37 of 170
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:13:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5388021
            Orig. end                         :: 5399470
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11449
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11449
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11449
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6449](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6449
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    375..389
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    731..746
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.24"
                     /tool="antismash"
     gene            complement(980..1651)
                     /locus_tag="NRO40_RS23545"
                     /old_locus_tag="NRO40_23545"
     CDS             complement(980..1651)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888536.1"
                     /locus_tag="NRO40_RS23545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23545"
                     /product="DUF3000 domain-containing protein"
                     /protein_id="WP_058942564.1"
                     /transl_table=11
                     /translation="MAAAQEQFSDHSDGTEGTGGGDGDALPPVFRLAVDALRAARLRPE
                     LEVEPSPPPRRLAPYAYALEATAVQDGQDLADGRLVLLHDPAGHEAWHGTFRLVTLVRA
                     ELEPEMAADPLLPEVCWSWLTGALQARGLSYAEASGTVTRAGSHYFGGLAERRPATQIE
                     IRASWTPQEGRNGAPDTAAHLTAWGDLLCQVAGLPPSGPGPAEGTAGVVSLPQRRGPHH
                     P"
     gene            1757..2821
                     /gene="hemE"
                     /locus_tag="NRO40_RS23550"
                     /old_locus_tag="NRO40_23550"
     CDS             1757..2821
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972892.1"
                     /locus_tag="NRO40_RS23550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23550"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_058942563.1"
                     /transl_table=11
                     /translation="MSANTRPSGPPTQTYDSAFLRACRREPVPHTPVWFMRQAGRSLPE
                     YLKVREGIPMLESCMRPELVTEITMQPVRRHRVDAAIFFSDIVVPLKAIGVDLDIKPGV
                     GPVVAEPIRRRADLERLRALTPDDVAYVTEAIGMLTGELGATPLIGFAGAPFTLASYLV
                     EGGPSRNHENTKALMYGDPELWADLLDRLADITAAFLKVQIEAGASAVQLFDSWVGALS
                     PADYRRAVMPASAKVFDAVAGYGVPRIHFGVGTGELLGLMGEAGADVVGVDWRVPLDEA
                     VRRVGPGKALQGNLDPAVLFAPREAVEEQADRVLRAAADLEGHVFNLGHGVLPTTDPDA
                     LTRLVEHVHTRTAR"
     gene            complement(2893..3102)
                     /locus_tag="NRO40_RS23555"
                     /old_locus_tag="NRO40_23555"
     CDS             complement(2893..3102)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491669.1"
                     /locus_tag="NRO40_RS23555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23555"
                     /product="hypothetical protein"
                     /protein_id="WP_058942562.1"
                     /transl_table=11
                     /translation="MDESRGHADTYRGTPDAPQAPAEAGARGGPPFADCVLCGEPTEYP
                     ESTRGATLCPVCEWREAERTACSG"
     misc_feature    complement(3103..3118)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.97"
                     /tool="antismash"
     gene            3166..3903
                     /locus_tag="NRO40_RS23560"
                     /old_locus_tag="NRO40_23560"
     CDS             3166..3903
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018554825.1"
                     /locus_tag="NRO40_RS23560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23560"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_107115108.1"
                     /transl_table=11
                     /translation="MADWQTGAVTSNDTDRPASDTRDAAPQFVLPLVVRVEKAAPPART
                     DALQTAARAVLVLLTDERSTGDGAWARAVSDWQDARIRKVVRRARGAEWRRAERLPGIT
                     VTGEEAEVRVFPPVPLDGWPKDLARLQVSGTELDDPAGPPAPPPASGPGRAVLWLNPEL
                     RMSTGKEMAQVGHGAQLLWWAMDEGTRAAWREAGFPLAVRTAPPARWAALVGSDRPVVR
                     DAGFTEVAPGSATVVSDFPLGAA"
     gene            4062..4823
                     /locus_tag="NRO40_RS23565"
                     /old_locus_tag="NRO40_23565"
     CDS             4062..4823
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890188.1"
                     /locus_tag="NRO40_RS23565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23565"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_198549354.1"
                     /transl_table=11
                     /translation="MRHINGTALIIAALVATVAALAFPVWSYADRSGTGQANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAVERAPTQAIRDAGDHLVVGHTDLDQRAR
                     DVAAKLGVELPNQPTEQQQGWLRELSAASGVEYETKFANLLRNAHGKVFGLVAQVRHTT
                     RNSLIRQLASDANQTVLDHITMLEATGRVDFDAIANEAAGQATASPTGPPAPNGNLPPA
                     PVPAVPAPGQTFTSLPSARPGPPTSIETTRP"
     misc_feature    4079..4094
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.8"
                     /tool="antismash"
     gene            5001..6449
                     /locus_tag="NRO40_RS23570"
                     /old_locus_tag="NRO40_23570"
     CDS             5001..6449
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890187.1"
                     /locus_tag="NRO40_RS23570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23570"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_058942560.1"
                     /sec_met_domain="DUF692 (E-value: 6.4e-93, bitscore: 309.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLGIGIGWRPEIADAVERLPGVEWVEAVAENLCAGHLPASLVR
                     LRERGVTVVPHGVSLGLGGAGRPDPGRLARLAETATALGAPLVTEHVAFVRAGGPLTAS
                     PAMEAGHLLPVPRTREALDVLCENVRIAQEALPVPLALENVAALFSWPDDELTEGRFLT
                     ELVDRTGVRLLVDVANLHTNGVNRGDDPYEALAALPAGAIAYVHVAGGVQRDGVWHDTH
                     AHPVPPAVLDLLAELASRAAPPGVLLERDDDFPPEDELAAELATIRGTVTAPGRAAGGG
                     AAGGGAGRAVAGRAVTGRVVGTARGRAAGGGAAVSGPVAGGAALRDGVTAPPEGTAALP
                     EGTAVVRDGARERLGLAQAALLSALVAGTPAPEGFDGARLRVQAGALVRKRAGVVAKVA
                     PELPGLLGDGYRDAFAAYAGSRPMPGGYRQDALAFARYALDAGLPGDAAGRRRLYRWWA
                     ERSAPRPPGRAARLVHAARAALVR"
     gene            complement(6740..7453)
                     /gene="hemQ"
                     /locus_tag="NRO40_RS23575"
                     /old_locus_tag="NRO40_23575"
     CDS             complement(6740..7453)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="NRO40_RS23575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23575"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_058942559.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFRLREALPEDRSGYADE
                     VQDLFDQLAAKDVTVRGTYDLSGLRADADLMIWWHAETSDELQEAYNLFRRTRLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGFPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKAVADLVAGLA"
     misc_feature    7453..7466
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 11.63"
                     /tool="antismash"
     gene            complement(7464..8909)
                     /gene="hemG"
                     /locus_tag="NRO40_RS23580"
                     /old_locus_tag="NRO40_23580"
     CDS             complement(7464..8909)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470506.1"
                     /locus_tag="NRO40_RS23580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23580"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_058942558.1"
                     /sec_met_domain="DAO (E-value: 4.1e-10, bitscore: 38.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVDNVDTPHGRVVVVGGGIAGLAAAHRLLLAGRRVTLLEATGRL
                     GGKLHAGRIADAPVDLGAESILARRPEALELARAVGLGDRLQPPATTRASVWTRGALRP
                     MPQGHVMGVPGDARGLGDLLSAEGLRALEGDRAQPPTELGDDVAIGAFVAERMGREIVD
                     RLVEPLLGGVYAGDSYRISMRAAVPKLFEAARRHATLTDAVRAVQGAAAQVPAGPVFMG
                     VEGGVGRLPLAVADAVTALGGEIRTATPVDALTRRAGRDGGAGTWEVRTGTEVLHADAV
                     VLATPAPAASRLLAAVSPAAAAELGAIEYASMALVTLAFRRADLAGLPEGSGFLVPPVD
                     GRTIKASTFSGHKWKWVDEGSGDLFVLRASVGRYGEEEHLERDDADLVTASLRDLGDAV
                     GLAARPVATEVTRWVGGLPQYPVGHLGRVARIRDAVAALPALRVCGAAYDGVGVPAVVA
                     DAWRAADEIIATPTLARSTESDE"
     gene            9045..10331
                     /locus_tag="NRO40_RS23585"
                     /old_locus_tag="NRO40_23585"
     CDS             9045..10331
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981756.1"
                     /locus_tag="NRO40_RS23585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_23585"
                     /product="DUF4349 domain-containing protein"
                     /protein_id="WP_079047106.1"
                     /transl_table=11
                     /translation="MPNRTRPRHACAAVLIGATLALAGCGAGADGGTSSTADRAAAPEA
                     ARGDADAGSAYGPESARGGAGGAKGAAPAAVQQHVIRTAELSVEVVDAGEALARARDAA
                     AKAGGHVADESTRRRQGPDGADGMTSRIVLRVPQAAYEQVLGELSGAGRLLSRKAEAKD
                     VTDQVVDVGSRVATQRASVERVRKLMDQATRLSDVVTLEQELSTRQAALESLLAQQAAL
                     KDRTALATITLELSERRTPQKPAEKDDPGPLDALAGGWDALAATVKWVVIVLAALAPWL
                     AVAALLYVLWRVFGRPWRARRAARRPAPAAAARPVPGRGPVPAYASAPTHAPVPAQAPT
                     HAPTPAAAPVPASASTSGTDADAGTGTSTGTGTEGGPDAGTDAGSGTSTGTGTGTEGRP
                     GAGTDADAGAGTGAKSTDAGRPGTGRGAD"
     misc_feature    complement(11105..11120)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
ORIGIN
        1 gcgggcgagg tggctcttca ccgtgagggc ggacaggccc atggagacgc cgatcgcctt
       61 gttcgactgg ccctccgcca ccagtcggag cacctcgacc tcgcggccgg acagctcgcg
      121 gtagccgccc gggtggctcg gggcacccgg ggggcggcgg tggaggcggg cggccgcgga
      181 gccgatgggg gcggcgccgg gccgggtcgg gtggccgatg ttggtgcggg tgccggtgac
      241 gacgtatccc ttgacgccgc cggcgagggc gttgcggacg gcgccgatgt cgtcggcggc
      301 ggacagggcc aggccgttgg gccagccggc ggcacgggtc tcggacagga gggtgagtcc
      361 ggaaccgtcg ggcaggtgga cgtcggccac gcagatgtcg cgcgggttgc cgattcgggg
      421 gcgggcctcc gcgatggacg aagcctcgat gacgtcacgc acgcccaggg cccacagatg
      481 gcgggtcacg gtggagcgga cgcgcgggtc ggccacgacg accatggccg tcggcttgtt
      541 cgggcggtag gcgaccaggc ttgcgggctg ctcgaggaga acggacacca ggcctcctgg
      601 gaaggtgcgg aacactgccg gcccgggatg gccggggcga gccgtgtgga agggtcacag
      661 acctcttcgg cagcaatcgc gtccgccttt agaggaagat cacgatttgg tgagtaacaa
      721 ttggggcaat tcggacgggt gatcgatcat cctacgaatg agccgtcacc cgtaccccga
      781 ccgcctccct ctcaccagga ggcgcccagg ctccctccgg acgaggggcg cacgaccgcc
      841 cggaccgtca tccgccggtt gacgcgacga ccggcgcacc gcgaggtccg cccgcccgcc
      901 ggctccctgg acggcggcgc gggtcccaag ggcggggcgg gtcccgaggg cggggcgggt
      961 cccgagggcg gggcgggtcc tacgggtggt gcgggccgcg gcgctggggg agggagacga
     1021 ccccggccgt gccctccgcc gggcccggcc ccgacggcgg cagccccgcc acctggcaca
     1081 gcaggtcacc ccacgccgtc aggtgcgccg ccgtgtccgg cgcgccgttg cggccctcct
     1141 gcggcgtcca ggacgcccgg atctcgatct gcgtcgccgg acggcgctcc gccagtccgc
     1201 cgaagtaatg ggacccggcc cgcgtgaccg tcccgctcgc ctccgcgtac gacagccccc
     1261 gtgcctggag cgcacccgtc agccacgacc agcacacctc cggcagcagc gggtccgccg
     1321 ccatctccgg ctccagctcg gcccgcacca gcgtcaccag ccggaacgtc ccgtgccagg
     1381 cctcgtgccc cgcggggtcg tgcagcagca ccagccggcc gtccgcgagg tcctggccgt
     1441 cctggacggc cgtcgcctcc agcgcgtacg cgtacggggc gagccgccgg ggcggcggcg
     1501 acggctccac ctcgagctcc gggcggagcc gcgccgcccg gagcgcgtcg accgcgagcc
     1561 ggaaaacggg cggcagcgcg tcaccgtccc cgccgcccgt gccctcggtg ccgtcggagt
     1621 gatcggagaa ctgttcctga gccgcagcca tgcggggaag agtaggcgga acggggcccg
     1681 cgcgcaggga gggacacccg cccccgcgcg ggccgccggg cacggccgcg cgcccgcccg
     1741 tgcgaagatt ctgggcgtga gtgccaacac ccgcccctcg ggccccccga cgcagacgta
     1801 cgactccgcc ttcctccggg cgtgccggcg cgagccggtg ccgcacacgc cggtctggtt
     1861 catgcgccag gcggggcgct cgctccccga gtacctgaag gtgcgcgagg gcatccccat
     1921 gctggagtcc tgcatgcggc ccgagctggt cacggagatc acgatgcagc ccgtgcgccg
     1981 gcaccgggtg gacgcggcga tcttcttcag cgacatcgtc gtgcccctca aggcgatcgg
     2041 tgtcgacctc gacatcaagc ccggggtcgg ccccgtcgtc gccgagccga tccgccgccg
     2101 cgcggacctg gagcggctgc gcgcgctcac gccggacgac gtcgcctacg tcaccgaggc
     2161 gatcggcatg ctcaccggcg agctcggcgc caccccgctg atcggcttcg cgggtgcgcc
     2221 cttcaccctc gcgagctacc tcgtcgaggg cggcccctca cgcaaccacg agaacaccaa
     2281 ggccctgatg tacggcgacc ccgagctgtg ggccgacctg ctggaccggc tcgccgacat
     2341 caccgccgcc ttcctcaagg tccagatcga ggccggcgcc tccgccgtgc agctcttcga
     2401 ctcctgggtg ggcgccctct ccccggccga ctaccgccgc gcggtcatgc ccgcctcggc
     2461 gaaggtcttc gacgcggtcg ccgggtacgg cgtgccccgc atccacttcg gtgtcggcac
     2521 cggcgagctg ctgggcctca tgggcgaggc gggcgccgac gtcgtcggcg tcgactggcg
     2581 cgtcccgctg gacgaggccg tccgccgcgt cggccccggc aaggcgctcc agggcaacct
     2641 cgacccggcc gtcctcttcg ccccgcgcga ggccgtcgag gagcaggccg accgggtgct
     2701 gcgggcggcg gccgacctgg agggccacgt cttcaacctc ggccacggcg tactgccgac
     2761 gaccgacccc gacgcgctga cgcggctcgt ggagcacgtc cacacccgca ccgcgcggta
     2821 gcccgtcccg ggccgagccc gtcgcgcccc cgggccagcg gaaacgtcat gtcggcctga
     2881 tgggccgcgc tcctagcccg agcaggcggt gcgctcggcc tcccgccact cacagacggg
     2941 gcagagcgtg gcgcccctgg tcgactccgg gtactcggtc ggctccccgc agagcacgca
     3001 gtcggcgaac ggcggcccgc cccgggcgcc cgcctccgcc ggtgcctgcg gcgcgtccgg
     3061 cgtgccgcgg tacgtgtccg cgtgacctcg gctctcgtcc atgtgtacga ctgtagtcct
     3121 ccgccctctc ccggcgcggg tcccggagcc gtctccggac gcccggtggc cgactggcag
     3181 actggggccg tgaccagcaa cgacaccgac aggcccgcga gcgatacccg ggacgccgcg
     3241 ccccagttcg tcctgcccct ggtggtgcgg gtggagaagg ccgccccgcc cgcccgcacc
     3301 gacgcgctgc agaccgcggc gcgggccgtg ctggtgctgc tgacggacga gcgctccacg
     3361 ggcgacggcg cgtgggcccg ggcggtcagc gactggcagg acgcccgcat ccgcaaggtc
     3421 gtccggcggg ccaggggcgc ggagtggcgc cgtgccgagc ggttgcccgg catcacggtc
     3481 accggggagg aggccgaggt gcgggtcttc ccgcccgtgc ccctcgacgg ctggccgaag
     3541 gacctcgccc gcctccaggt ctccggcacc gagctcgacg accccgcagg gccgcccgcg
     3601 cccccgcccg cgtcggggcc gggacgtgcc gtgctgtggc tcaacccgga actgcgcatg
     3661 tcgaccggca aggagatggc gcaggtcgga cacggcgcgc agctcctgtg gtgggccatg
     3721 gacgagggga cccgcgccgc ctggcgggag gcgggcttcc cgctcgcggt ccgtacggca
     3781 ccgcccgccc gctgggccgc cctggtgggc agcgaccgcc ccgtcgtgcg cgacgcgggt
     3841 ttcacggagg tcgcccccgg ctcggcgacc gtggtctccg acttcccgct cggcgcggcc
     3901 tgacggcgcg cggtccggtg gcgcggtccg gtggcgcccg ggccggtggc gcgctgcggc
     3961 gggccgtgga gcgccggcgt cgggggcgtt gaacgccccg cccgtgccgt acgtacctcc
     4021 cagcgccgtg aggcaggtcg aacgggtcgg gaggcacagt gctgcggcac atcaacggaa
     4081 cggcactcat catcgcggcg ctcgtcgcca cggtcgcggc gctcgcgttc cccgtctggt
     4141 cgtacgccga ccggtcgggc accgggcagg ccaacctcaa cgcgtcgtcc gtggccacgc
     4201 agtggggccc gctgtcggcc accgaccggg acttcctcgt gaaggtgcgg ctggccggac
     4261 tgtgggagct gcccgccggg cagcaggccg tcgagcgggc cccgacgcag gccatcaggg
     4321 acgcgggcga ccacctcgtg gtcggccaca ccgacctcga ccagcgcgcc cgcgacgtcg
     4381 cggcgaagct cggcgtggag ctgccgaacc agcccaccga gcagcagcag ggctggctgc
     4441 gggagctgtc cgcggcgagc ggggtcgagt acgagaccaa gttcgccaac ctgctgcgca
     4501 acgcccacgg gaaggtcttc ggcctggtcg cgcaggtccg gcacaccacc cgcaacagcc
     4561 tgatccgcca gctcgcctcg gacgccaacc agaccgtcct ggaccacatc accatgctgg
     4621 aggcgaccgg cagggtcgac ttcgacgcca tcgccaacga ggccgccggc caggccaccg
     4681 ccagtcccac cgggccgccg gcccccaacg gcaacctgcc gcccgcgccg gtccccgccg
     4741 tgcccgcgcc cgggcagacg ttcacgtccc tgccgtcggc ccggccgggc ccgccgacct
     4801 ccatcgagac cacccgcccg tgagacccgg ggcgcccccg cgcccgccgg tccgccggcc
     4861 cttcgtcctc cgccgccggt ccgccgcccc gtcggccgtt cccgccgaag ctcaaatgaa
     4921 gctctgaaag cggcggggcg cggttggcgg cccccgtgac ggggccatga cccctccagc
     4981 ggtacggacg agggggagac atgacggggc tcggcatcgg catcgggtgg cgtccggaga
     5041 tcgcggacgc ggtggagcgg ctgcccgggg tggagtgggt ggaggccgtc gcggagaacc
     5101 tctgcgccgg acacctcccc gcctccctgg tgcggctgcg cgagcggggc gtcaccgtgg
     5161 tcccgcacgg cgtgtcgctc gggctgggcg gcgccgggcg gcccgacccg ggacggctgg
     5221 cgcggctcgc ggagacggcg accgccctgg gcgcgccgct ggtgaccgag cacgtcgcgt
     5281 tcgtccgggc cggtgggccg ctcaccgcgt cgccggcgat ggaggccggc cacctgctgc
     5341 cggtgccgcg gacccgggag gcgctggacg tgctctgcga gaacgtccgc atcgcccagg
     5401 aggcgctgcc cgtgccgctc gccctggaga acgtggcggc gctgttctcc tggcccgacg
     5461 acgaactgac ggagggccgg ttcctcacgg agctggtcga ccgcaccggc gtccgcctcc
     5521 tcgtcgacgt ggcgaacctg cacaccaacg gggtcaaccg cggcgacgac ccgtacgagg
     5581 cgctcgccgc gctgccggcc ggtgccatcg cgtacgtgca cgtggcgggc ggcgtgcagc
     5641 gcgacggcgt gtggcacgac acgcacgccc atcccgtgcc gccggccgtc ctggacctgc
     5701 tggcggagct ggcgtcgagg gcggcgccgc ccggggtgct gctggagcgc gacgacgact
     5761 tcccgccgga ggacgagctg gcggcggagc tggccacgat ccgcggcacg gtcaccgcgc
     5821 cgggacgggc ggcgggtgga ggggcggcgg gtggaggggc cggacgggcg gtggcagggc
     5881 gggcggtgac gggtcgcgtg gtgggaacgg cgaggggccg ggcggcgggt ggaggggcgg
     5941 cggtgtcggg gccggtcgcg ggtggcgcgg cgctgcggga cggcgtgacg gcgccaccgg
     6001 aagggacggc ggcactgccg gagggcacgg cggtcgtgcg ggacggggcc cgggagcggc
     6061 tggggctggc ccaggcggcg ttgctgtcgg cgctcgtggc ggggacgccc gcgccggagg
     6121 ggttcgacgg ggcgcgtctg cgggtgcagg ccggggcgct ggtgcgcaag cgggccgggg
     6181 tggtggcgaa ggtcgccccc gagctgcccg ggctgctcgg cgacggctac cgtgacgcgt
     6241 tcgcggcgta cgcgggctcc cgcccgatgc cgggcggcta ccggcaggac gccctggcct
     6301 tcgcccggta cgcgctcgac gcgggtctgc ccggggacgc ggccgggcgg cgccggctgt
     6361 accggtggtg ggcggagcgg tcggcccccc gcccgccggg ccgggcggcg cggctggtgc
     6421 acgccgcccg cgcggcgctg gtgcggtgac acccctcaac cgcacgaaaa tcggtgacac
     6481 cctcggccgg gcgggggagc ggcggcgcgg gggaggggcg ggtgggacga ccctggtgcg
     6541 aggccgtggg agtgcaaagt gccctggcgc ggaggccctg gggggtgacg ccggtgcggc
     6601 gcgcagggaa ctgcgcgccg cacccggagc cggacccggt gtcgctggag cggcggacgg
     6661 ttcgcgtccg ccgtctgatc ttccgggtcg gggggcggcg gccggctgga tgcccggccg
     6721 ccaccgtgct gtcgggccgt caggccaggc ccgccaccag gtccgcgacc gccttgcggc
     6781 ggccggtgta gaacgggacc tcctcgcgga cgtgcatccg cgcctcgctg gcccgcaggt
     6841 ggcgcatgag gtcgacgatg cggtacagct cgtccgcctc gaaggcgagg atccactcgt
     6901 agtcgccgag ggagaacgag gcgaccgtgt tggcgcggac gtcggggaag ccgcgggcca
     6961 tcttgccgtg gtcggcgagc atgcggcggc ggtcctcgtc gggcagcagg taccagtcgt
     7021 acgagcgcac gaacgggtac acgctgacgt agtcgcgggg cgtctcgtcg gcgaggaacg
     7081 ccgggatgtg cgacttgttg aactcggccg ggcggtgcag cgccatgttc gaccacacgg
     7141 gctccagcgc gcggccgagg cgggtgcggc ggaagaggtt gtacgcctcc tggagttcgt
     7201 ccgaggtctc cgcgtgccac cagatcatca ggtcggcgtc ggcgcgcagt ccggagaggt
     7261 cgtaggtgcc gcggacggtg acgtccttgg cggccagctg gtcgaacagg tcctggacct
     7321 cgtcggcgta gccgctccgg tcctcgggca gagcctcgcg cagtcggaag acggaccaca
     7381 gggtgtagcg gatgacctcg ttgaggtcct tcgccttctt accggcgttc gggatctttt
     7441 cgggcgcact catagggcta ttctcactcg tcgctctcgg tgctccgcgc cagggtcggc
     7501 gtggcgatga tctcgtccgc ggcccgccac gcgtccgcga ccaccgccgg gacgcccacg
     7561 ccgtcgtacg ccgccccgca cacccgcagc gcggggagcg cggcgaccgc gtcgcggatc
     7621 cgggcgaccc ggcccaggtg accgacgggg tactgcggca ggccgccgac ccaacgggtg
     7681 acctcggtgg ccaccggccg cgcggcgagc ccgacggcgt cccccaggtc ccgcagcgac
     7741 gccgtgacca ggtcggcgtc gtcccgctcc aggtgctcct cctcgccgta ccggccgacg
     7801 gaggcgcgca ggacgaagag gtcgccggac ccctcgtcga cccacttcca cttgtgcccg
     7861 gagaacgtgg acgccttgat cgtgcggccg tcgaccggcg gtaccaggaa gccggagccc
     7921 tcgggcaggc cggcgaggtc ggcgcgccgg aaggcgaggg tgacgagggc catcgaggcg
     7981 tactcgatcg cgcccagctc cgcggcggcg gccggcgaca ccgcggcgag gagccgcgac
     8041 gcggccgggg cgggggtcgc gaggaccacc gcgtcggcgt gcaggacctc cgtccccgta
     8101 cggacctccc acgtcccggc gcctccgtcc cgcccggcac ggcgggtgag ggcgtcgacg
     8161 ggggtggcgg tgcggatctc gccgccgagg gcggtgaccg cgtcggcgac ggcgagcggc
     8221 aaccgcccca ccccgccctc gacgcccatg aagaccgggc cggcgggcac ctgggccgcc
     8281 gcgccttgca cggcgcggac ggcgtcggtc agggtggcgt ggcggcgggc ggcctcgaac
     8341 agcttcggca cggcggcccg catggagatc cggtacgagt cgcccgcgta caccccgccg
     8401 aggaggggtt cgacgagccg gtcgacgatc tcgcggccca tccgctcggc gacgaacgca
     8461 ccgatggcga cgtcgtcgcc gagctcggtg ggcggctgcg cgcggtcgcc ttcgagcgcg
     8521 cgcagcccct ccgccgagag caggtcgccg agcccgcggg cgtcgccggg gacgcccatg
     8581 acgtgcccct ggggcatcgg ccgcagcgcg ccccgggtcc agacggacgc acgggtcgtg
     8641 gcgggcggct ggagccggtc gccgaggccg acggcgcggg ccagttcgag cgcctcggga
     8701 cggcgggcca ggatcgactc ggccccgagg tcgacggggg cgtccgcgat ccgcccggcg
     8761 tggagcttgc cgccgagccg accggtggct tccagcagcg tcacgcgccg ccccgcgagc
     8821 aggaggcggt gggcggcggc gagtccggcg atgccgccgc ccacgacgac gacgcgtccg
     8881 tggggcgtgt ccacgttgtc cacgttcatg ggtccactct ctcaaacgcc gtgacgcggc
     8941 ccgaccgtga ccctttcgga accgggaaag ggcaaccacg tcccccgtcc cgcgcgtcga
     9001 accggcgtca ctggacacgg cacgagacac cttgggggac atccatgccg aaccgaaccc
     9061 gaccgcggca cgcctgcgcg gccgtcctga tcggcgccac gctggccctc gccgggtgcg
     9121 gcgccggcgc ggacggcggc acgtcgtcga ccgccgaccg ggccgccgcg cccgaggcgg
     9181 cccgggggga cgcggacgcc ggctccgcgt acgggcccga gtcggcccgc ggcggcgcgg
     9241 gcggcgcgaa gggggcggcg ccggccgcgg tgcagcagca cgtcatcagg acggcggagc
     9301 tgtcggtgga ggtggtggac gcgggcgagg cgctggcgcg ggcgcgggac gccgcggcga
     9361 aggcgggcgg ccacgtcgcc gacgagtcga cacggcgccg ccagggcccc gacggggccg
     9421 acgggatgac gtcgaggatc gtgctgcgcg tcccgcaggc ggcgtacgag caggtgctcg
     9481 gcgagctcag cggcgcgggc cggctgctgt cgcggaaggc ggaggcgaag gacgtcaccg
     9541 accaggtggt ggacgtgggc agccgcgtcg ccacccagcg ggccagcgtg gagcgggtcc
     9601 ggaagctgat ggaccaggcc acgcggctga gtgacgtggt gacgctggaa caggagctga
     9661 gcacccgcca ggccgcgctg gagtcgctcc tcgcccagca ggccgccctg aaggaccgta
     9721 cggcgctggc cacgatcacc ctggagctct cggagcggcg gaccccgcag aagccggcgg
     9781 agaaggacga ccccggcccg ctggacgccc tggcgggcgg ttgggacgcg ctggccgcga
     9841 cggtgaagtg ggtggtgatc gtcctggcgg cgctggcccc gtggctggcg gtggcggcgc
     9901 tgctgtacgt cctgtggcgg gtgttcggcc gcccgtggcg ggcccgcagg gcggcccggc
     9961 ggccggcccc cgctgcggcc gcccggcccg tcccgggacg cggtcccgta ccggcgtacg
    10021 catccgcacc cacccacgca ccggtgccgg cccaggcgcc gactcacgcc ccgaccccgg
    10081 ccgcggctcc cgttcccgcc tccgcgtcca cgtccggcac ggacgcggac gccggtacgg
    10141 gcacgagcac gggcacgggc acggaggggg gcccggacgc cggcacggac gcgggctccg
    10201 gcacgagcac cggcaccggc acgggcacgg aggggcgtcc gggcgccggc acggacgcgg
    10261 acgccggcgc gggcacgggc gccaagagca cggacgccgg gcgcccgggg acggggcgcg
    10321 gggcggactg aacggccccg accccgtagc gtgttccgca tgggacgtgt gggacggcga
    10381 cagcggttgg tggtggtcgg gggtgacgcg gcggggatgt ccgccgcgtc acaggcccgg
    10441 cggctgaagg gggtcgacga cctggagatc gtcgcgttcg agcgcggcgg cttctcgtcg
    10501 tactcggcct gcggcatccc gtactgggtg ggcggtgacg tggaggggcc cgatcggctc
    10561 gtcgcgcgca ccccgcagga gcaccgcgcc cgcgacatcg acctgcggct gcggaccgag
    10621 gtgaccgagc tcgacctgga cggacggcgc gtccgggccc gggacctcga ctccggggag
    10681 gagcagtgga cgggcttcga ccggctcgtg ctcgcgaccg gggcgcggcc ggtgcgcccg
    10741 ccgctgccgg gcatcgacgc gccgggcgtg cacggcgtgc agaccctcgg cgacggacag
    10801 gccctgctcg acaccctggc cgcgacgccc gggcgccgcg cggtcgtggt cggcgccggg
    10861 tacatcggcg tggagatggc cgaggcgctg ctcaagcgcg gctacgaggt gaccgtcctc
    10921 aaccggggcg agcagcccat ggcgaccctc gacccggaca tgggccggct ggtgcacacg
    10981 gccatggagg ggatgggcat cgacgtcgtc gccggcgccg gggtcaccgc gatccggacc
    11041 ggagcggacg gccgggtgcg ggcggtggag gccggggacg ccacgtaccc ggccgacgtc
    11101 gtcgtgctcg gcatcggcgt cgagccggag acgtccctgg cccgggcggc gggcctgccg
    11161 ctcggcgtgt acggcgggct gctgaccgac ctgtcgatgc gggtgcgcgg gcacgaggac
    11221 gtgtgggcgg gcggcgactg cgtggaggtg ctcgacctgg tctccggccg cgaacggcac
    11281 gtcccgctcg gcacgcacgc caacaagcac ggccaggtga tcggggcgaa cgtgggaggc
    11341 ggctacggca ccttcccggg cgtggtcggc acggcggtga gcaaggtctg ctcgctggag
    11401 atcgcccgca cgggcctgcg cgaacgcgac gcgcgcgagg tggggctgc
//
