LOCUS       NZ_CP102332            10537 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces changanensis strain HL-66 chromosome, complete genome.
ACCESSION   NZ_CP102332
VERSION     NZ_CP102332.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces changanensis
  ORGANISM  Streptomyces changanensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Streptomyces changanensis sp. nov., an actinomycete isolated from
            soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) College of Forestry (Academy of Forestry),
            Northwest A&F University, Northwest A&F University, Yangling,
            Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 187.45x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024600715.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 19:18:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,071
            CDSs (total)                      :: 5,982
            Genes (coding)                    :: 5,812
            CDSs (with protein)               :: 5,812
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 70 of 170
            Pseudo Genes (incomplete)         :: 131 of 170
            Pseudo Genes (internal stop)      :: 9 of 170
            Pseudo Genes (multiple problems)  :: 37 of 170
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:13:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3696258
            Orig. end                         :: 3706795
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10537
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="10"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10537
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10537
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5537](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5537
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(846..1502)
                     /locus_tag="NRO40_RS16455"
                     /old_locus_tag="NRO40_16455"
     CDS             complement(846..1502)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132116.1"
                     /locus_tag="NRO40_RS16455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16455"
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="WP_058940921.1"
                     /transl_table=11
                     /translation="MPNTPPPAAPPGTPVKATLPAGTPLFRVHRSEWAATSFNPVPADR
                     LWGGGRFDATEDDPYRFLYAGATAAAAVCESFLRSVPYTDPGPRIVPRAAVRGRRLAFL
                     RLAVDVDVVSLMSGRDLAAVAQDSWLVQAEAHEYAFTRAWGHWIRRHTDPWARGFVWPS
                     KREPADRVTVLFGDRCPPGVVEETGTPAVDLAGRDGEEWLNGLLAPYHARVGGPR"
     gene            complement(1508..2275)
                     /locus_tag="NRO40_RS16460"
                     /old_locus_tag="NRO40_16460"
     CDS             complement(1508..2275)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984705.1"
                     /locus_tag="NRO40_RS16460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16460"
                     /product="hypothetical protein"
                     /protein_id="WP_058940868.1"
                     /transl_table=11
                     /translation="MSGEEHRTADPYEVLARAAAEWDDLEPRLDAAGRARLGDALRGLR
                     EGPRPWDAARDAVRVLAAALPGGAGARFVAPTVPDVPPVPGRPPVEGGSGAPGGSPVEG
                     GSGAPGAGAGAPYSASYAGFRAVDLAALVLDGTRMVGPVLGPVRDRLLAVPALSEDAVR
                     ERGGDPYGPGLVRLRGPGGAVRLPRFQFAGDALPWPVVLEVNRLLDADDDPWAAADWWL
                     SRDAWLAAPPAALLGTGRDGTLVDAARALVDGG"
     gene            complement(2414..3475)
                     /locus_tag="NRO40_RS16465"
                     /old_locus_tag="NRO40_16465"
     CDS             complement(2414..3475)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838032.1"
                     /locus_tag="NRO40_RS16465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16465"
                     /product="hypothetical protein"
                     /protein_id="WP_058940869.1"
                     /transl_table=11
                     /translation="MPTGPLPDHASLLGEVRAVRRAGLVRLRELDLPALSRAAAAFDRG
                     PGTTPVERLLRAAVSRLDAGTLRDAAAYALGLAQGTRDWPAADRRRRAAEVYGVSVERF
                     RKHHEPLALGQVAEQIAAVAAERAPAAAPTAHPGATHRVLAAGGHRVTLHVHPVDLLND
                     VDVVVSPANVWLALPEPYKSSVPASLRRAGAVRGPTGELVEDLVHDGLRAWAARHGAAG
                     RPVQPATVAATEAGALAAQGVRRIYHAAVAVPRAGTNDYDVLPADVTRAVTRVFALLRE
                     ESARFDPPLRSLCLPLLGAGRGGLTAATSAAAVWAAVGAELARGGRWEVHLVVLDPADA
                     DALARVVEGGGGA"
     gene            complement(3546..4280)
                     /locus_tag="NRO40_RS16470"
                     /old_locus_tag="NRO40_16470"
     CDS             complement(3546..4280)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239817.1"
                     /locus_tag="NRO40_RS16470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16470"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_232790970.1"
                     /transl_table=11
                     /translation="MATTRDTNTGSVPAPSPAPDGAPGAPGAPTGRATVPASRRGTGPA
                     AGPRDADGATGAGRAFAWLLVVTGAAGVLAAWVITLDKFRLLEDPHYTPGCSLNPVVSC
                     GSVMKSAQATVFGFPNPMLGLVTYGMVVAVGAGLLAGARHRRWFWLGLQAGTLFGVGFC
                     AWLAQQSLYVINALCLWCCLAWVATAVMFWAVTAYNVGRGHLPAPRGVRRFFGEFPWIP
                     PVLHLGVVGMLVLTRWWDFWTG"
     gene            complement(4304..4807)
                     /locus_tag="NRO40_RS16475"
                     /old_locus_tag="NRO40_16475"
     CDS             complement(4304..4807)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889043.1"
                     /locus_tag="NRO40_RS16475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16475"
                     /product="DUF5949 family protein"
                     /protein_id="WP_058940870.1"
                     /transl_table=11
                     /translation="MTSSPAVTPDRRHSPLGTLTVIPWCSEPTDDDPGEPMLMVYSLGD
                     GRDGPEAGEEAMRAQLERLGLSIGERLVDLGRDSRIPITLLVEAEQAVLTLPFMKVQCP
                     VPPEWQRAAHTKGQVYLIFAVRPWPEAVPGVEVSEERLRAFVTGDGLLEGSAHCLAPVL
                     RVRT"
     gene            5001..5537
                     /locus_tag="NRO40_RS16480"
                     /old_locus_tag="NRO40_16480"
     CDS             5001..5537
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028525.1"
                     /locus_tag="NRO40_RS16480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16480"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_257375437.1"
                     /sec_met_domain="melC (E-value: 1.7e-46, bitscore: 156.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAKSPQPLLAARLRRRGPLRRGPQRGGPVRRGVLRALFTAAVAS
                     FTGGALARVATTPHDGPGGPPTGGSGPGRAPAPGAGRPVHGAFDETYRGRRIQGRPVTA
                     AVPEFEVLVDGRPLHLMRCAGGGYLTLVNHYETYPTPLAATRAAVDELGGARLARSVGH
                     GAALRREGGGHGAHA"
     gene            5521..6387
                     /locus_tag="NRO40_RS16485"
                     /old_locus_tag="NRO40_16485"
     CDS             5521..6387
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="NRO40_RS16485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16485"
                     /product="tyrosinase family protein"
                     /protein_id="WP_058940871.1"
                     /transl_table=11
                     /translation="MAHTRKDQRDLSAAERRRFVAAVLELKRAGRYDDFVRTHIAYYGA
                     DGESGLRTAHMAPSFLPWHRQYLLEFERALQEVDAGVTVPYWDWTRDRSPVGQPWGEDF
                     LGGNGRRGDLRVTTGPFAYATGDWTVNVGVTEDRFLTRDFGHPKDPVELPTRDELAWAT
                     RETVYDVAPWDSTSPGGFRNRLEGWVPGRGSARLRNHNRVHRWVGGLMLGAASVNDPVF
                     WLHHAFVDAVWSRWQRRNPGARYLPAAPPPRGSAQHGRVVARNEPMPPWDVTPAQLADH
                     SRLYRYA"
     gene            complement(6479..6811)
                     /locus_tag="NRO40_RS16490"
                     /old_locus_tag="NRO40_16490"
     CDS             complement(6479..6811)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672799.1"
                     /locus_tag="NRO40_RS16490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16490"
                     /product="chaplin"
                     /protein_id="WP_058940872.1"
                     /transl_table=11
                     /translation="MSRIAKAAVIIAGTGAVVLSGAGMALADAEANAAAVNSPGVLSGN
                     VLQAPIHIPVNICGNTINAVGLLNPAFGNVCINNGGKDHKEKSDDGKGGHHGGHYDGKG
                     ENGYGS"
     misc_feature    7015..7030
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.58"
                     /tool="antismash"
     gene            complement(7049..7567)
                     /locus_tag="NRO40_RS16495"
                     /old_locus_tag="NRO40_16495"
     CDS             complement(7049..7567)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239813.1"
                     /locus_tag="NRO40_RS16495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16495"
                     /product="hypothetical protein"
                     /protein_id="WP_257375438.1"
                     /transl_table=11
                     /translation="MKSQVGAGILATCLASAAAAALATPAAAAEAPVDLPLQSLGGVLP
                     VTPPTVSTAMPLLTPGMPQDLGHVGEGGLPELTLLPRTPIGSEFPETRVGAPMPSLLGG
                     RPLGTALLTSPRSDVETSAPGASLGEPLSLPRGGEPGLPGVRLPKAAALSPTLSGVLES
                     DLGLSPQEG"
     misc_feature    7602..7617
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.52"
                     /tool="antismash"
     misc_feature    7653..7667
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.08"
                     /tool="antismash"
     misc_feature    7707..7718
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.49"
                     /tool="antismash"
     gene            complement(7747..8022)
                     /locus_tag="NRO40_RS16500"
                     /old_locus_tag="NRO40_16500"
     CDS             complement(7747..8022)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529518.1"
                     /locus_tag="NRO40_RS16500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16500"
                     /product="hypothetical protein"
                     /protein_id="WP_058940874.1"
                     /transl_table=11
                     /translation="MKPTKVAAVVAGSVMAMGMAAPAFAADTLTPSSLNGAVDTLGKRG
                     LTEAVPSGGVDKVADNKVVGKVADTAGGLNAADKAAPLLGGLPLGK"
     gene            complement(8308..8868)
                     /locus_tag="NRO40_RS16505"
                     /old_locus_tag="NRO40_16505"
     CDS             complement(8308..8868)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NRO40_RS16505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NRO40_16505"
                     /product="hypothetical protein"
                     /protein_id="WP_058940875.1"
                     /transl_table=11
                     /translation="MRLLTSRRVALAAVCAALTLGTAGPALADAPQTRPDAPTAVAADT
                     DATDDATAAVQAAVDELIASMTKDQATTGMPENLPESVQKLIDAVLGTVGGLTGVSMPD
                     LPVDVPSTQLPQAPGTDTPATQDPAAGTTTPGIALPEITVPEVVRPDVPITGVSSDWFP
                     DADFFTPQAPVTLPQPPAATTTG"
     misc_feature    complement(8951..8964)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.87"
                     /tool="antismash"
     gene            complement(9137..9376)
                     /locus_tag="NRO40_RS16510"
                     /old_locus_tag="NRO40_16510"
     CDS             complement(9137..9376)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="NRO40_RS16510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_16510"
                     /product="chaplin"
                     /protein_id="WP_058940876.1"
                     /transl_table=11
                     /translation="MSRIAKAAVLTSAVAASVAGAAGVANADADAHAAAVGSPGVISGN
                     IVQVPIHIPINLCGNTIDIIGLLNPTFGNVCIND"
     misc_feature    complement(9474..9489)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    9589..9603
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 13.73"
                     /tool="antismash"
ORIGIN
        1 tgaccggccc ggccgacgcc cggcgggtcg cccgctgcct cgtccacctc gcccgctggc
       61 accagttgca cgccctggac aacccggcct cgcccctcgc cggcctcgtc cgcgtcgagg
      121 tcagcccgtg gacgccaggc cggtacgcgc ccgccctcac ccccgacgcg gccggtgagc
      181 tcgtcctcca ctacgccggc cgcccgggcg cctggcaccg ccccgaggtc gcgatccgcg
      241 tccacaacca cgggccccgc ccgctgtggg cggtgctcgt cgacctgacc gacagccacg
      301 ccgcccacac cggtctgtac gagggacact tcatcggccc gcacggcacg gcccacgtct
      361 tcgacggcga cccggtcgaa ctgtccctcc cgccgacccg ccccgagcgc ccgggcgccg
      421 aggtccgcga ctggttcaag ctgatcgtcg ccgaggggga gctcaacacc gcgcccttcc
      481 gcctgccccc gtgggacccg cacgccgacg cccgcgacgc gccctccggc ccgcccgtcg
      541 acggcgtcct ccacctcgca ggggccggcg gaggcggttc cggcggtacc ggtggtgcgc
      601 gcgacaccgg gccgtcccgg cgtccggcgc ccggccagtg gtggacgacc accgtgcccg
      661 tccgcacggt cgtcccacgg ccctccggcc ggggcacctg agacccggcc cgccctcgcc
      721 cggcggcgag cccggcccgt cgtccccggt ggcatccggc ccccgacgcc cgtatcggcg
      781 cccgtccccg acgcccgtcc cggccgtccg tcccgcccgt cccggccgcc cgccgccggc
      841 acggttcagc gggggccgcc gacccgggcg tggtacgggg cgaggagacc gttcagccac
      901 tcctcgccgt cccgcccggc gaggtccacg gcgggcgtac cggtctcctc gaccaccccg
      961 ggcgggcagc ggtcgccgaa gaggaccgtg acccggtcgg cgggctcccg cttcgacggc
     1021 cacacgaagc cccgcgccca cgggtcggtg tggcgccgga tccagtgccc ccacgcgcgc
     1081 gtgaaggcgt actcgtgcgc ctccgcctgg accagccagg agtcctgcgc gaccgccgcc
     1141 aggtcccgcc cggacatcag cgacacgacg tccacgtcca cggcgagccg caggaacgcc
     1201 agccggcggc cgcgcaccgc ggcgcgcggc acgatccggg gtcccgggtc ggtgtacggc
     1261 accgaccgaa ggaacgactc gcacaccgct gcggcggccg tggcaccggc gtacaggaag
     1321 cggtacgggt cgtcctcggt cgcgtcgaac cggccaccgc cccacagccg gtcggcgggg
     1381 acggggttga aggaggtggc ggcccactcc gaccggtgca cccggaacag cggggtgccg
     1441 gcggggagcg tggccttcac gggcgtaccg gggggcgcgg cgggcggcgg cgtgttgggc
     1501 acggccctca ccccccgtcc acgagggcgc gggcggcgtc cacgagcgtg ccgtcccgac
     1561 cggtgccgag cagcgcggcg ggcggtgcgg cgagccaggc gtcgcgggac agccaccagt
     1621 cggcggcggc ccacgggtcg tcgtcggcgt ccagcaggcg gttgacctcc agcaccacgg
     1681 gccagggcag cgcgtccccg gcgaactgga agcgcggcag ccgcaccgcg ccccccggcc
     1741 cgcgcagccg gaccagcccg ggcccgtacg ggtcgccgcc ccgctcccgc accgcgtcct
     1801 ccgacagcgc gggcaccgcc agcagccggt cgcgcaccgg cccgagcacg ggaccgacca
     1861 tccgcgtgcc gtcgagcacc agcgcggcga ggtccacggc acggaacccg gcgtacgagg
     1921 cggagtacgg cgccccggcc ccggcccccg gtgcccccga accgccctcc acaggggagc
     1981 cccccggcgc ccccgaaccg ccctccacag gtggccggcc cggcaccggc ggcacgtccg
     2041 gcaccgtcgg ggcgacgaac cgcgcccccg ccccgcccgg cagcgcggcg gccagtacgc
     2101 gcaccgcgtc gcgggccgcg tcccacgggc ggggcccctc acgcaggccc cgcagcgcgt
     2161 cgcccagtcg tgcccggccg gcggcgtcga gccgcggctc caggtcgtcc cactcggcgg
     2221 ccgcccgcgc cagcacctcg tacggatcgg cggtccggtg ctcctcaccg ctcatgccgc
     2281 accccttcca gcggccccac ccggggctga ccgctctcgc cccgccccac ccgagccccc
     2341 ctccggcccc actggcggca taccgctcac gccccccacc cgccccccgc cggccccacc
     2401 gacagctcac cactcacgcc ccgccgcccc cctccaccac acgcgccagc gcgtcggcgt
     2461 cagccgggtc caggaccacc aggtgcacct cccaccggcc gccgcgcgcc agttccgccc
     2521 cgacggccgc ccagacggcg gcggcgctgg tcgcggcggt cagtccgccc cgcccggccc
     2581 cgagcagggg caggcacagc gatcgcagcg gcgggtcgaa gcgcgcggac tcctcccgga
     2641 gcagggcgaa gacccgggtc accgcgcggg tcacgtcggc gggcaggacg tcgtagtcgt
     2701 tggtgcccgc cctcggcacg gccaccgccg cgtggtagat ccggcggacc ccctgcgcgg
     2761 cgagcgcgcc cgcctccgtg gcggcgacgg tcgccggctg gaccggccgg ccggcggcgc
     2821 cgtgccgggc ggcccaggcc cgcagcccgt cgtggaccag gtcctcgacg agctccccgg
     2881 tcgggccgcg caccgcgccg gcgcgccgca gcgacgcggg gacggacgac ttgtacggct
     2941 cgggcagcgc gagccacacg ttggccggcg acacgacgac gtcgacgtcg ttgagcaggt
     3001 ccaccgggtg gacgtgcagc gtgacccggt gcccgcccgc cgccagcacg cggtgcgtgg
     3061 ccccggggtg cgcggtcggc gcggcggccg gggcccgttc ggcggccacg gccgcgatct
     3121 gctcggcgac ctgaccgagc gccaggggtt cgtggtgctt gcggaagcgt tcgacgctca
     3181 cgccgtagac ctcggcggcc ctgcgccgcc gatcggcggc gggccagtcg cgggtgccct
     3241 gggcgaggcc cagggcgtag gcggcggcgt cccgcagcgt ccccgcgtcc agccgggaca
     3301 ccgcggcgcg caacagccgt tccaccggag tggtgccggg gccgcggtcg aaggcggcgg
     3361 cggcgcggga gagcgcgggc aggtccagct cgcggaggcg gacgaggccc gcgcgccgta
     3421 cggcccgcac ctccccgagg agcgatgcgt ggtcaggcag ggggccggtc ggcatggacg
     3481 gaagcatccc aacacgccga gcggtcccac aacccgcttc gcgtacctcg tcccgccggg
     3541 ggccgtcagc cggtccagaa gtcccaccag cgggtcagca ccagcatccc cacgacgccg
     3601 aggtgcagca ccggggggat ccacgggaac tcgccgaaga agcgccgtac cccccgcggg
     3661 gcgggcaggt ggccgcggcc gacgttgtac gcggtgacgg cccagaacat gacggccgtc
     3721 gccacccagg ccaggcagca ccacaggcac agggcgttga tcacgtacag ggactgctgc
     3781 gcgagccacg cgcagaagcc gacgccgaac agcgtcccgg cctggaggcc cagccagaac
     3841 cagcgccggt ggcgggcccc ggcgagcagg ccggcgccga ccgcgacgac cattccgtac
     3901 gtgacgaggc ccagcatggg gttggggaat ccgaagaccg tggcctgcgc gctcttcatg
     3961 acgctgccgc acgacacgac ggggttcagg ctgcaccccg gcgtgtagtg cgggtcctcc
     4021 aggagccgga acttgtcgag ggtgatgacc caggccgcga gcacccccgc cgcgcccgtc
     4081 accaccagca gccaggcgaa cgcccgcccc gctcccgtcg ccccgtccgc gtcacgcggc
     4141 ccggcggccg ggccggtgcc gcgacgggag gcggggacgg tcgcccggcc ggtgggggcg
     4201 ccgggagcgc cgggagcgcc gtccggggcg ggggaggggg cggggacgga tcccgtgttc
     4261 gtgtcgcgcg tggtcgccac ggtgctcacc ggtcctctcg gtgtcaggtc cgcacccgca
     4321 gcacgggggc gaggcagtgg gcgctgccct ccaggagccc gtcgccggtc acgaaggcgc
     4381 gcagccgctc ctccgacacc tcgacgcccg gcaccgcctc cggccacggg cgcaccgcga
     4441 agatcaggta gacctggccc ttggtgtgcg cggcgcgctg ccactccggc ggcaccgggc
     4501 actgcacctt catgaacggc agtgtcagga cggcctgttc ggcctccacc agcagggtga
     4561 tggggatccg gctgtcgcgg ccgaggtcca cgaggcgctc gccgatcgac aggcccagcc
     4621 gctccagctg cgcccgcatc gcctcctcgc cggcctccgg gccgtcccgc ccgtcgccga
     4681 gcgagtacac catgagcatc ggctcacccg ggtcgtcatc ggtcggctcg ctgcaccagg
     4741 ggatgacggt gagcgtcccc aggggggaat gtcgcctgtc cggggtcacg gcgggggaag
     4801 aggtcatggc gccggatact agtggggcga atgagctcac tcccggcaca atccacccgt
     4861 tcggccgata cccgttcggc tcgattcacc ttgacgtgtc ggggattcgg ggcctcgcgc
     4921 tcggaacggt tcgtggatgg cgtcgttgtc ccccggggga gggagtccac cgtccgcacg
     4981 agccgtacgg gagatcgccc atgatcgcca agagcccgca gccactcctc gccgcccgcc
     5041 tcaggcgccg ggggcccctg cgccgcggac cacagcgcgg gggaccggtg cgccggggcg
     5101 tcctgcgcgc gctgttcacc gccgccgtcg cgtcgttcac cggtggcgcg ctcgcgcgtg
     5161 tcgccaccac cccgcacgac ggcccaggcg ggccgccgac cggcggctcc gggcccgggc
     5221 gcgcgccggc cccgggcgcc gggcggcccg tgcacggcgc cttcgacgag acgtaccggg
     5281 gccgccgcat ccagggcagg cccgtcacgg cggccgtacc ggagttcgag gtgctcgtcg
     5341 acgggcggcc gctgcacctg atgcgctgcg cgggcggcgg ctatctcacc ctggtgaacc
     5401 actacgagac gtacccgacg ccgctcgccg cgacccgcgc cgccgtcgac gagctgggcg
     5461 gggcgcggct ggcccggtcc gtcgggcacg gcgcggcgct gcgccgggag gggggcggcc
     5521 atggcgcaca cgcgtaagga ccagcgcgac ctgagcgcgg ccgagcggcg caggttcgtc
     5581 gccgcggtgc tggagctgaa gcgcgccggc cggtacgacg acttcgtccg cacgcacatc
     5641 gcgtactacg gcgcggacgg cgagagcggg ctgcggaccg cgcacatggc gccgtcgttc
     5701 ctgccgtggc accgccagta cctgctggag ttcgagcggg ccctgcagga ggtcgacgcg
     5761 ggcgtgaccg tgccgtactg ggactggacc cgcgaccgca gcccggtggg ccagccgtgg
     5821 ggcgaggact tcctcggcgg caacggccgg cgcggcgacc tccgcgtcac gaccggcccc
     5881 ttcgcgtacg ccacgggcga ctggaccgtc aacgtcggcg tcaccgagga tcggttcctc
     5941 acgcgcgact tcggccaccc caaggacccc gtcgagctgc ccacgcgcga cgagctggcg
     6001 tgggcgactc gggagaccgt gtacgacgtg gcgccctggg actccaccag ccccggtggc
     6061 ttccgcaacc ggctggaggg gtgggtgccc ggccgcggca gcgcccggct gcgcaaccac
     6121 aaccgcgtgc accgctgggt gggcggactc atgctgggcg cggcgtcggt caacgacccg
     6181 gtgttctggc tgcaccacgc gttcgtggac gccgtctggt cgcgctggca gcgccgcaac
     6241 ccgggcgccc gctacctgcc cgccgccccg ccgccgcgcg ggtcggcgca gcacgggcgg
     6301 gtcgtggcgc ggaacgagcc gatgcccccg tgggacgtga cgccggccca gctggccgac
     6361 cacagccgcc tgtaccggta cgcctgacgg gtgcgggtac ggaagtggcc cccggcggtg
     6421 cgccgggggc cacttccgta ccggggagcg gcacggggag caggagggaa cgggagcgtc
     6481 agctgccgta gccgttctcg cccttgccgt cgtagtggcc gccgtggtgg ccgcccttgc
     6541 cgtcgtcgct cttctccttg tggtccttgc cgccgttgtt gatgcagacg ttgccgaacg
     6601 cggggttgag gagaccgacg gcgttgatgg tgttgccgca gatgttgacc gggatgtgga
     6661 tcggcgcctg gagcacgttg cccgacagga caccgggcga gttgacggcg gcggcgttgg
     6721 cctccgcgtc ggcgagggcc atgccggccc cgctcaggac gacagcgccc gtgccggcga
     6781 tgatgacggc agccttcgcg atgcgagaca ttcaggaact ccttgtcgat gggaacacga
     6841 ccgcggacca gcgggcgtca ccccgcttca acgcggattc tcacgtatgg taacggcttg
     6901 ctcgctctcc gtaccgtaac gggttggtgc ggagcgcccg cggacgggcc gcgggtcggt
     6961 gaccatgcga caagccgtcg ggtgtaacga gcggcggtcg gacgggtggc gggaagaacg
     7021 ctttcttcac tcgcaccctc cggcgtgttc acccctcctg cggggacagt ccgaggtcgg
     7081 actccaggac gccggagagg gtgggggaga gggcggccgc cttcggcagc cggacgcccg
     7141 gcaggcccgg ctcccctccc cgcggcaggg agagcggctc gccgagggag gcgcccggcg
     7201 cggacgtctc cacatcggag cgcggcgagg tcagcagggc cgtaccgagc gggcggccgc
     7261 cgaggaggct cggcatcggc gctccgacgc gggtctcggg gaactcgctg ccgatcggcg
     7321 tgcgcggcag cagggtgagc tccgggaggc cgccctcgcc gacgtggccg aggtcctgcg
     7381 gcatgccggg cgtgagcagc ggcatggcgg tgctcacggt cggcggggtg accgggagga
     7441 cgccgcccag ggactgcagc gggagatcca ccggcgcttc cgcggcggcc gcgggggtgg
     7501 ccagtgccgc ggcggccgcc gaggcgaggc acgtggcgag gatccccgca ccgacctgag
     7561 acttcacgtt catcttctgc gtacgccttc ccggacgtgt gaacgggaat tcgtttctgc
     7621 gttgcggtag cgagcaccgc cgcggaaggg cgtggaattc gtctgcttgc agtagtgccc
     7681 ggaacacggg aacggggggc cgggcggaaa acgttgtcgt ccccgtcagg cccccgttcc
     7741 ctcgggttac ttgccgagcg gcaggccgcc caacagcggt gcggccttgt cggcggcgtt
     7801 caggccgccg gccgtgtcgg ccaccttgcc gacgaccttg ttgtcggcca ccttgtcgac
     7861 cccgccggag ggaaccgcct cggtcagacc ccgcttgccc agcgtgtcga ccgccccgtt
     7921 gaggctggag ggcgtgaggg tgtcggcggc gaaggcgggg gcggccatgc ccatcgccat
     7981 cacggagccg gcgacgaccg cggcaacctt cgtgggcttc atcgggagag tccttcctgg
     8041 aaaggcatcg tgcgtggcaa ctcgcgccgc cgaaccggta cttggccggg gcgggtcggc
     8101 gctctctgcc ctctgtaacg agccctcggc gcgccggaaa ctccgtcggc ggttatttct
     8161 cgccactgca cccgaatgaa gtgcggcagt cggataatcc gaccagtgtg aaaggacgtc
     8221 aaacgaaacg gagggccggg tcccgcgccg tctcggcgga acgggacccg gcccggtacg
     8281 gcgtcgtgcc cgagcgcgga cccaccgcta accggtcgtg gtggcggccg ggggctgggg
     8341 gagcgtgacg ggcgcctgcg gggtgaagaa gtccgcgtcc gggaaccagt cgctggagac
     8401 gcccgtgatc gggacgtccg gccgcacgac ctcggggacg gtgatctccg gcagggcgat
     8461 ccccggcgtc gtggtacccg cggcggggtc ctgggtcgcg ggcgtgtcgg tgccgggggc
     8521 ctgcgggagc tgcgtgctcg gcacgtcgac ggggaggtcg ggcatcgaga cgcccgtgag
     8581 gcccccgacg gtgccgagga cggcgtcgat gagcttctgc accgactccg ggaggttctc
     8641 gggcataccg gtcgtcgcct ggtccttggt catggaggcg atcagctcgt ccaccgccgc
     8701 ctgcacggcg gcggtggcgt cgtccgtggc gtcggtgtcg gcggcgacgg cggtgggcgc
     8761 gtccgggcgg gtctgcggtg cgtcggccag ggcggggccc gcggtgccga gggtgagcgc
     8821 ggcgcagacg gcggcgagcg cgacgcggcg ggatgtcagg aggcgcatgc gcgagttctt
     8881 ccttccgggt gggacggcgg actggtgtcc tcaccgtgcg ccgcccggcg ggcgcccgca
     8941 agcgcgtacg acacggaggg tgactgcgtc ccacgggtga acgcacccgc cctgagctgc
     9001 ggtgacgcgc cgccgcaccg gtcgtggcgg ccggtgcggg cgcgccgcca agagggtggc
     9061 cggacacacg gcggcgccgc cccggcaagg atcacggggc ggcgccatcg cgtgggggga
     9121 gatcccggcg gggtcgtcag tcgttgatgc agacgttgcc gaaggtcggg ttcagcaggc
     9181 cgatgatgtc gatggtgttg ccgcagaggt tgatcgggat gtggatcggc acctggacga
     9241 tgttgcccga gatgacaccg ggggagccca cggccgcggc gtgcgcgtcg gcgtcggcgt
     9301 tcgcgacgcc ggcggcaccc gccacggacg cggctacggc ggaggtcagg acggcagcct
     9361 tcgcgatacg ggacatggtg gagaactcct aggtttcggg tgaagcggtg cggccgacgc
     9421 ggccggcggc atgaacaacg catgagcgct cccgccgttc cgccaccgaa cgggtgacgc
     9481 ggacgtgccg ggccaggccg ggagcgcggt gccggggtgt gtaccgcgtg atgcaagggg
     9541 tgccgcgtgg tgtcgaggga tgccgtggag tgaccggggt ggtggcgaac gtgtcagata
     9601 aacggtcgtg ttgctcccta tatgttctca atgcttcgtg ttttgctcat ggggttataa
     9661 cgtgcgtgct gttgcgtcga tccatcccgg tgggatccgt ttcgtcactc ctcaacgcgg
     9721 agaaccccta tgcggaatcc agtggtgccc gccgtgcggc gcgggctggt ctgcgtcctt
     9781 ttttgtgccg ccctgtcagc cgtactcccc gccaccgccg cgccccgcgt gaaggagcag
     9841 cagcgcattc cgttcgccca gcggtaccac gcggtccagc acggcggtct ggcgcgcgcc
     9901 gcgaattcgg cgatcacctg tagggaatcc gccgcgaacg accgtgccgc gaacctcccc
     9961 tgcctttccg cgcggggcgg caagagcgct gccgcgaacc acgccttcga catgttctac
    10021 acggacgtgg acgacgaccc gaacaccttc aactccagcc gcgcggagct cgtcctgccg
    10081 aaggacgccc aggtcacgta cgcccggctg tactggggcg gcaacctccg cgtgggcgag
    10141 cagaagccgc cggaggacaa cgggcgggtc ctcgtcgcgg aacccggcgg cgcctacaag
    10201 gccgtcctcg ccgattccgt catcggccac cgcacggccg acggggccga cgccttccaa
    10261 gccaccgcgg acctcacgga actcgtccgg agctccggcc ccggcatgta caccgtcgcc
    10321 cagatcaacg tcgccatggg gcgttcggga gtgggcgcct ggggtggttg gacgctcgtc
    10381 gtggcgtacg cgaagaaagg cgccccgctg cgccacctcg ccctctggga cggattcgag
    10441 acactcaccc gcgaccggcg ttccgtggac gtggaactcg gccgcatgcg cgttcccgtg
    10501 ggcggatcgg gacgcctcgg gatcgtcgcc tacgacg
//
