LOCUS       NZ_CP102332            10399 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces changanensis strain HL-66 chromosome, complete genome.
ACCESSION   NZ_CP102332
VERSION     NZ_CP102332.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces changanensis
  ORGANISM  Streptomyces changanensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Streptomyces changanensis sp. nov., an actinomycete isolated from
            soil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6817869)
  AUTHORS   Wu,H. and Han,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) College of Forestry (Academy of Forestry),
            Northwest A&F University, Northwest A&F University, Yangling,
            Shaanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 187.45x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024600715.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 19:18:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,071
            CDSs (total)                      :: 5,982
            Genes (coding)                    :: 5,812
            CDSs (with protein)               :: 5,812
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 70 of 170
            Pseudo Genes (incomplete)         :: 131 of 170
            Pseudo Genes (internal stop)      :: 9 of 170
            Pseudo Genes (multiple problems)  :: 37 of 170
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:13:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1375565
            Orig. end                         :: 1385964
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP102332.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    439..452
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(892..2400)
                     /locus_tag="NRO40_RS06085"
                     /old_locus_tag="NRO40_06085"
     CDS             complement(892..2400)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.5"
                     /locus_tag="NRO40_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06085"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_198549431.1"
                     /transl_table=11
                     /translation="MELRQLEYFVAVAEEHGLGRAAERLRTTAEAVAGRIGALEGDLGL
                     RLFEPAAPGHVRLTAAGERLLPEARAALAAAGRVRETAAGIAGGTDGLVRLGTSRAFSD
                     RVYRALGVLAERRPRLRVRQERAPQDARLAAVRSGTFDAALVRAVRRVDGMVLYPVWTE
                     PLLVALPAGHPLAAADELHPEQLARLPLRLAPRAVNPAFHDLVTGALPHWTPGPPFTTL
                     RATLDDLAGAAEPSWTVFHPVGPLPRDRRLAFRALRAPRVPVSLAVRAGGLVPAVQALL
                     EALTGTTDPPPAPRPVSVPTPLAAPPRPAPRTAPRPAPLPATVRAAPARSAAPAAGAAG
                     VSRPPCGTPVAAPAPALRASGDRRDATARTGTPGATHAPPPQGPAPTGMPVPAPGGAAT
                     QGPVPASTHVPADMPAAAQAPVPAQVPVPVPASAQAPADMPVPAQGPVVAQAPVPAQIP
                     APVQAPVPVQAPVPARGGLPVHAPVPADAPGRSCPDAACPDPPP"
     misc_feature    2616..2632
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.12"
                     /tool="antismash"
     misc_feature    complement(2745..2760)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.64"
                     /tool="antismash"
     misc_feature    complement(2773..2788)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.21"
                     /tool="antismash"
     misc_feature    2782..2797
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            2952..3479
                     /gene="ectA"
                     /locus_tag="NRO40_RS06090"
                     /old_locus_tag="NRO40_06090"
     CDS             2952..3479
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718915.1"
                     /locus_tag="NRO40_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06090"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_058944214.1"
                     /transl_table=11
                     /translation="MTAAPTDLVPARSEFIDSPRVEDGAALWRIARDSEVLDLNSSYSY
                     LLWCRDFAATSVVARGADGEPVAFVTGYVRPDRPGTLVVWQVAVDHGHRGRGLAGTLLD
                     ALVARLADRGIHTVETTVTPDNTASDRLFTSFAERRGASLEREVLFDGELFPEGGHEPE
                     VLYRIRPVAAAG"
     gene            3622..4869
                     /gene="ectB"
                     /locus_tag="NRO40_RS06095"
                     /old_locus_tag="NRO40_06095"
     CDS             3622..4869
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 7.6e-71.
                     Score: 230.8. Matches aSDomain:
                     nrpspksdomains_NRO40_RS06095_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="NRO40_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06095"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_058944230.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.2e-76, bitscore:
                     254.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAFENLESEVRSYCRGWPTVFDHARGARLTDEDGRSYLDFFAGA
                     GSLNYGHNNPVLKRALIDYLERDGVTHGLDMATTAKRAFLETFHDLVLRPRDLPYKVMF
                     PGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHG
                     TPMPFDNYLDGRVPDFLWFERLLQDSGSGLNQPAAVIVETVQGEGGINVARPEWLRALR
                     ELCDRRDMLLIVDDIQMGCGRTGGFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFRPE
                     LDVWGPGEHNGTFRGNNPAFVTATAALRAYWADGGTQRQTLARGEQVEQALLTICDENS
                     ADGAHYRGRGLVWGLEFADKERATRICRRAFELGLLLETSGPESEVVKLLPPLTVTPEE
                     LDEGLTTLARAVRETA"
     aSDomain        3688..4695
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NRO40_RS06095_Aminotran_3.1"
                     /evalue="7.60E-71"
                     /label="NRO40_RS06095_Aminotran_3.1"
                     /locus_tag="NRO40_RS06095"
                     /protein_end="358"
                     /protein_start="22"
                     /score="230.8"
                     /tool="antismash"
                     /translation="DHARGARLTDEDGRSYLDFFAGAGSLNYGHNNPVLKRALIDYLER
                     DGVTHGLDMATTAKRAFLETFHDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYLDGRVPDFLWFERL
                     LQDSGSGLNQPAAVIVETVQGEGGINVARPEWLRALRELCDRRDMLLIVDDIQMGCGRT
                     GGFFSFEEAGITPDIVTLSKSISGYGLPMSLCLFRPELDVWGPGEHNGTFRGNNPAFVT
                     ATAALRAYWADGGTQRQTLARGEQVEQALLTICDENSADGAHYRGRGLVWGLEFA"
     gene            5001..5399
                     /locus_tag="NRO40_RS06100"
                     /old_locus_tag="NRO40_06100"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="NRO40_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06100"
                     /product="ectoine synthase"
                     /protein_id="WP_058944213.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-54, bitscore:
                     181.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLKDIENTERHVRAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGETHWIEPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5403..6320
                     /gene="thpD"
                     /locus_tag="NRO40_RS06105"
                     /old_locus_tag="NRO40_06105"
     CDS             5403..6320
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477733.1"
                     /locus_tag="NRO40_RS06105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06105"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_058944212.1"
                     /sec_met_domain="PhyH (E-value: 5.5e-38, bitscore: 129.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTPNPTAATTVPSAAAGVPDLYPTRGATEVVTPRQDPVVWGP
                     LTPELESFERDGFLAVDRLLTPDEVATYRAELDRLVTDPAVRADERSIIEPKSQDVRSV
                     FEVHRISDVFARLVSDERVVGRAREILGSDVYVHQSRVNVKPGFGASGFYWHSDFETWH
                     AEDGLPRMRTVSVSIALTENRDTNGALMIMPGSHRTFLGCAGETPKDNYKKSLQMQDAG
                     TPSDEALTRFADAHGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENT
                     AVEPFAAPVRRPEFIGARDFTPVR"
     gene            complement(6595..7665)
                     /locus_tag="NRO40_RS06110"
                     /old_locus_tag="NRO40_06110"
     CDS             complement(6595..7665)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045205.1"
                     /locus_tag="NRO40_RS06110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06110"
                     /product="alkene reductase"
                     /protein_id="WP_058944211.1"
                     /transl_table=11
                     /translation="MTTAFDPVDLAGTPLRNRVVMAPMTRSRAPGRVPTELMAEYYAQR
                     ASAGLIISEGIQPTPEGQGYPDTPGLHFAEQVAAWRRVTDAVHAAGGRIFAQLMHAGRI
                     GHPSVLADLPGDLHPVAPSAVAAAGQLYTAEGLKDYPEPRELTGAQVRQVIEGFAAAAR
                     NAVEAGFDGVELHGANGYLIHQFLAPGTNRRTDEWGGSVDDRVRFAVEAVKAVAAAVGA
                     ERTALRISPGNPFNDIDEPDPLPAYTALVAELDPLGLAYLHVLEASPEVRDVTTQLRKQ
                     YSGTLVLNPATDGPTDHGVLTLVEDGTADLLSFGALFLANPDLPARLAAGGPYNEPDRA
                     TLYGGDHRGYTDYPTL"
     gene            7795..8439
                     /locus_tag="NRO40_RS06115"
                     /old_locus_tag="NRO40_06115"
     CDS             7795..8439
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238580.1"
                     /locus_tag="NRO40_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06115"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_079047359.1"
                     /transl_table=11
                     /translation="MPARPPAAASHRSAPAAARRDGTPSAARGGPVSMTLSRVARLHRV
                     AAGRFLRAAGLYPGQEFVMMHLWDHGPVRQSELIRVLDLDPSTVTKMLQRLEQGGHVRR
                     APDPADRRAVLVAATDAGCALHAQVAAAWTELEDHSLAALTPDERRELARLLGKVEGGL
                     CKPAPLCPDPQGPGSPGAGPPPEPTAQPADAGAPGSPPADAGAAGPAADAR"
     gene            complement(8483..9562)
                     /locus_tag="NRO40_RS06120"
                     /old_locus_tag="NRO40_06120"
     CDS             complement(8483..9562)
                     /NRPS_PKS="Domain: Aminotran_5 (15-342). E-value: 3.6e-22.
                     Score: 70.7. Matches aSDomain:
                     nrpspksdomains_NRO40_RS06120_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019885285.1"
                     /locus_tag="NRO40_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06120"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_058944229.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.1e-16, bitscore:
                     56.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METPLGGDEFAPAQTYLNTSTCGLLPRRAVEAVKRLAEENAAGGP
                     DGVGSFDTVAAARAAFARLAGVDPGRVALGASVSAHVGLVAASLPPGAEVLYAEGDFSS
                     VMTPFVVRGDLRTRSAPLEELAGAVREDTHLVAFSSVQSADGRVADMPAIRAAAAAHGA
                     RTLLDASQSAGWLPLDAGAYDYTVAGAYKYLVCPRGASFLTVTEEAQESLVPIHAGWFA
                     GEDRYGSCYGPVQRLAPDARRFDEPPSYLAYHGAEQALEVLLEVGVEEIGRHATALAAR
                     FREGAEALGYAPVPAASVVVAVPGLGHLRPALAREGVVTSARAGNLRVSFHLYNTAADV
                     DRALDALDAVGGLDAVAAG"
     aSDomain        complement(8537..9517)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NRO40_RS06120_Aminotran_5.1"
                     /evalue="3.60E-22"
                     /label="NRO40_RS06120_Aminotran_5.1"
                     /locus_tag="NRO40_RS06120"
                     /protein_end="342"
                     /protein_start="15"
                     /score="70.7"
                     /tool="antismash"
                     /translation="YLNTSTCGLLPRRAVEAVKRLAEENAAGGPDGVGSFDTVAAARAA
                     FARLAGVDPGRVALGASVSAHVGLVAASLPPGAEVLYAEGDFSSVMTPFVVRGDLRTRS
                     APLEELAGAVREDTHLVAFSSVQSADGRVADMPAIRAAAAAHGARTLLDASQSAGWLPL
                     DAGAYDYTVAGAYKYLVCPRGASFLTVTEEAQESLVPIHAGWFAGEDRYGSCYGPVQRL
                     APDARRFDEPPSYLAYHGAEQALEVLLEVGVEEIGRHATALAARFREGAEALGYAPVPA
                     ASVVVAVPGLGHLRPALAREGVVTSARAGNLRVSFHLYNTAADVDR"
     gene            complement(9632..10339)
                     /locus_tag="NRO40_RS06125"
                     /old_locus_tag="NRO40_06125"
     CDS             complement(9632..10339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019765416.1"
                     /locus_tag="NRO40_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NRO40_06125"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_058944210.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEEGLAAFAHRDDVEVVYRSFELDP
                     GRARGDVAPVVDMLAAKYGRTREEARAMEEHVASVARGQGLEYRVEGREHGGTFDVHRL
                     LHLAKARGRQEALLDLAYRANFAEDRSVYETEVLVRLAVEAGLDEDEARAVLADAAAYA
                     DDVRADEREAAELGATGVPFFVLDRRYGVSGGQPAEVFTRALEQAWEGRVPQPVDTASG
                     DAAACGADGTCAV"
ORIGIN
        1 tccacacggc ggccaccgcc gagcaggtcg ccggggcgct ggagcggcac ccggagatcg
       61 acttcgtgtc gctggtgcac gcggaagccg cgaccggcaa caccaacccg gtcgcggaga
      121 tcggcgaggt ggtccgggcg cacggcgcgc tgttctacct ggacgcggtg gcctcgatcg
      181 gcgcggagcc ggtgctgccg gacgcgtggg gcgtcgacct gtgcatcatc ggcgcgcaga
      241 aggcgatggg cggcccggcg ggtgtctccg ccgtctcggt cagcccgcgc gcgtgggagc
      301 ggttcgcggc gaacccggcc gcgccgcgcc gctcgtacct ctccctcctg gactggaagg
      361 agcgctggat cgacggcggc cgcagggcgc tgctccacgc gcccgcgcag ctggagatgc
      421 tggcgctgga ggcgtgcctg gaccgtatcg acgcggaggg actggacgcg ctcaccgcgc
      481 ggcacgcgtc ggccgcggcg gccacccgcg cgggcgtgct cgcgctgggg ggcggcctgg
      541 agccgtacgt gcacgaggcg cgggaggccg cccccgtcgc gacgacgctg cgcaccccgg
      601 ccggggtgga cgcctccctg ctggtggccg ccgcgctgga ggcggcgccc gcctccggcc
      661 ccggcggtgc ggccccggcg ctgcccctgg cggcgggcgg cggggcgctg gccgcggaga
      721 tggtccgggt gaaccactac gggcccgccg ccaccccggg caccgtgcgg gcgtgcctga
      781 cggccctggg cgccgccctg gccgcgacgg gccgggaggt cgacacggac gcggcgctgc
      841 gggcggcggc ggaggcgtgg cggtagggac ggctcgtccg ccgggggcgt gtcagggcgg
      901 cgggtcaggg caggccgcgt cagggcagga ccggcctggc gcgtcggccg ggaccggggc
      961 gtgtaccggg agcccgcccc gagccgggac cggggcctga accgggaccg gggcctgaac
     1021 cggggccggg atttgcgccg ggaccggggc ctgcgccacg accgggccct gcgccgggac
     1081 cggcatgtcg gccggggcct gtgccgaagc cgggaccggg accgggacct gcgccgggac
     1141 cggggcttga gccgcggccg gcatgtcggc cgggacgtgc gtcgaagccg ggaccgggcc
     1201 ctgcgtcgcg gctccgcccg gcgccgggac cggcatgccg gtcggagcag gtccctgtgg
     1261 gggcggggcg tgtgtcgcgc ccggggttcc ggtccgcgcg gtggcgtcgc gccggtcacc
     1321 ggacgcccgg agcgcgggtg cgggggccgc gaccggggtg ccgcacggcg gccgtgacac
     1381 gccggcggcc ccggcggccg gggccgcgga ccgggccggg gcagcccgta ccgtcgccgg
     1441 aaggggtgcc ggccggggtg ccgtacgcgg agccggccgg ggcggtgccg ccaggggggt
     1501 cggcacggac accggccgcg gcgcgggcgg cgggtcggtg gtgccggtga gcgcctccag
     1561 cagggcctgt accgccggaa cgagaccgcc cgcccgtacg gcgagcgaga ccgggacccg
     1621 gggcgcgcgc agggcgcgga aggccagccg gcggtcgcga ggcaggggcc ccaccgggtg
     1681 gaagaccgtc caggacggtt ccgcggcgcc cgccaggtcg tcgagggtgg cccgcagggt
     1741 ggtgaagggg gggccgggcg tccagtgcgg gagggcaccc gtgaccaggt cgtggaaggc
     1801 cggattcacc gcgcggggcg ccagtcgcag cgggaggcgc gccagctgct ccggatggag
     1861 ctcgtccgcc gcggcgagcg ggtgccccgc gggcagggcg acgagcagcg gttcggtcca
     1921 caccgggtac agcaccatcc cgtcgacgcg gcgcacggcc cgcaccagcg cggcgtcgaa
     1981 cgtcccggag cggaccgcgg cgaggcgcgc gtcctgcggg gcgcgctcct gccgcacccg
     2041 cagccggggg cgccgttcgg cgaggacgcc gagggcccgg tacacgcggt cggagaaggc
     2101 gcggctggtg ccgaggcgca ccaggccgtc cgtcccgccg gcgatgccgg ccgccgtctc
     2161 ccgcacgcgt cccgccgcgg cgagcgcggc ccgtgcctcg ggcagcaggc gttcgcccgc
     2221 ggcggtgagc cgtacgtgcc cgggggccgc cggttcgaag agccgcagcc ccaggtcgcc
     2281 ctccagcgcg ccgatccgcc cggccaccgc ctccgcggtg gtgcgcagcc gttcggcggc
     2341 gcgaccgagg ccgtgctctt cggcgacggc cacgaagtac tccagctgac gcagttccac
     2401 cgccgcgccc acccgcccct cactgatcgc tgccctgccg tccggtccgg tcaacgaccg
     2461 ggcggggacg gtctcttccc catgtgtacg ggcggacggc gtcgatgtgc gaacacgacg
     2521 cgcgggaggg tcgcggaacc ggacgcgggg gcccggcgcc gggcgggacg cgtccggcgg
     2581 caccggcgcc ccccaacgcg cgcccctatt gctgcggacg aagaattcgg cacttatttc
     2641 cgacgtactt cggctgtttt caccgaggta ttcgggtggg cagctcccca ctttctgctg
     2701 cccacatccg atgatgccaa acgccaagat tcgcgcacgc tcctgtgaac gaatcatgga
     2761 ggcccgacgg gcgtttcgag gtgtgatcga ttccacatcc gcagcattat gacccaattt
     2821 atcccggcaa atcggccaca accacaggcg ccgccggatc gtcccgtgta cgcgcgataa
     2881 cacagatgcg ccagccaccc aacaccttcc ggccatgcaa tccgaagaat tgctgggtaa
     2941 gttccatccg catgaccgcc gcaccaacag acctcgtacc tgcccgatcc gaattcatcg
     3001 acagcccacg agtggaggac ggcgccgcac tgtggcgcat cgcccgcgac tccgaggtgc
     3061 tggacctgaa ctcctcgtac agctacctgc tgtggtgccg ggacttcgcg gccacgtccg
     3121 tcgtcgcgcg cggcgcggac ggcgaaccgg tcgccttcgt gaccggctac gtccgccccg
     3181 accggcccgg gacgctcgtc gtctggcagg tcgccgtcga ccacggccac cgcggccggg
     3241 gcctggcggg cacgctgctg gacgccctcg tggcgcggct ggccgaccgc gggatccaca
     3301 ccgtggagac gaccgtcacg cccgacaaca cggcgtccga ccggctcttc acctcgttcg
     3361 ccgagcgccg gggcgcctcg ctggagcggg aggtcctctt cgacggggag ctgttcccgg
     3421 agggcggcca cgagcccgag gtgctctacc gcatccggcc ggtcgccgcg gcgggctgac
     3481 cgccgccgcc cccgcccccg ctcccccgcc cacgccccgc gacccccgca ggagggacca
     3541 gctgtgacca tcaccccgtc cgcccgaccg accgccaccc cgagccccgc cccgaccgcc
     3601 tacgacggcg gcgcgtcccc gatggccgcg ttcgagaacc tggagtcgga ggtgcgcagc
     3661 tactgccgcg gctggccgac cgtgttcgac cacgcgcggg gcgcccgcct caccgacgag
     3721 gacggccgct cgtacctgga cttcttcgcc ggcgcgggct ccctcaacta cggccacaac
     3781 aaccccgtgc tgaaacgagc gctgatcgac tacctggagc gggacggcgt cacgcacggg
     3841 ctggacatgg cgaccaccgc caagcgcgcc ttcctggaga cgttccacga cctcgtcctg
     3901 cgcccccggg acctgccgta caaggtgatg ttccccggcc cgaccggcac caacgccgtg
     3961 gagtcggcgc tgaagctggc ccggaaggtg aagggccgcg agtccatcgt gtcgttcacc
     4021 aacgccttcc acggcatgtc gctcggctcg ctcgccgtca ccggcaacgc cttcaagcgg
     4081 gccggcgccg gcgtcccgct ggtccacggc acgccgatgc cgttcgacaa ctacctcgac
     4141 ggccgggtcc cggacttcct gtggttcgag cggctgctgc aggacagcgg ctcggggctc
     4201 aaccagccgg ccgccgtcat cgtcgagacc gtgcagggcg agggcggcat caacgtcgcg
     4261 cggcccgagt ggctgcgcgc gctgcgggag ctgtgcgacc gccgcgacat gctgctgatc
     4321 gtcgacgaca tccagatggg ttgcggccgc accggcgggt tcttctcctt cgaggaggcc
     4381 ggcatcaccc cggacatcgt caccctgtcg aagtccatca gcggctacgg actgcccatg
     4441 tcgctctgcc tgttccggcc ggagctggac gtgtggggac ccggcgagca caacggcacc
     4501 ttccgcggca acaacccggc cttcgtcacc gcgacggcgg ccctccgcgc gtactgggcc
     4561 gacggcggca cgcagcggca gaccctcgcc cggggcgagc aggtggagca ggcgctgctg
     4621 accatctgcg acgagaactc cgcggacggc gcccactacc ggggccgcgg cctggtctgg
     4681 ggcctggagt tcgccgacaa ggagcgcgcg acgcggatct gccggcgcgc cttcgagctc
     4741 ggcctgctgc tggagacgtc cggccccgag agcgaggtcg tgaagctgct cccgccgctg
     4801 acggtcacgc ccgaggaact ggacgagggg ctgaccacgc tggcgcgtgc cgtccgcgag
     4861 accgcctgac cccggaagtc cgcaaccgcg ggaccgcacg ggaaccccgg cgccccgagc
     4921 gcgccgggcc ccgggagccg cgaaggcccc ggcccccgcg gtcccgcccc gtacgccgta
     4981 cgaaaggaag tagcaccacc gtgatcgtcc gatcgttgaa ggacatcgag aacaccgagc
     5041 gccacgtgcg ggcggcctcc ggcacctggg agtccaagcg catcgtgctc gccaaggaga
     5101 aggtcggctt ctcgctccac gagaccgtgc tgtacgccgg gaccgagacg tccatgtggt
     5161 acgcgaacca catcgaggcc gtgctgtgcg tcgagggcga ggcggagctg accgacgacg
     5221 agacgggcga gacccactgg atcgagcccg gcacgatgta cctgctcgac ggccacgagc
     5281 ggcacaccct gcgtccgaag accgacttcc ggtgcgtgtg cgtgttcaac ccgccggtca
     5341 ccgggcggga ggaccacgac gagaacggtg tgtacccgct gctcacggag gagggctgaa
     5401 ccatgaccac gaccaccccg aaccccaccg ccgccaccac cgtcccgtcc gccgccgcgg
     5461 gcgtaccgga cctgtacccg acccggggcg cgaccgaggt ggtcaccccc cggcaggacc
     5521 cggtggtctg ggggccactg acgcccgaac tggagtcgtt cgagcgtgac ggcttcctcg
     5581 cggtcgaccg gctgctcacc ccggacgagg tcgccacgta ccgcgccgag ctcgaccggc
     5641 tcgtcaccga cccggcggtc cgcgccgacg agcgctcgat catcgagccg aagtcccagg
     5701 acgtgcggtc ggtcttcgag gtgcaccgga tcagcgacgt gttcgcgcgg ctggtcagcg
     5761 acgagcgggt ggtgggccgg gcccgcgaga tcctcggctc ggacgtgtac gtccaccagt
     5821 cgcgcgtcaa cgtgaagccg ggcttcggcg cctcgggctt ctactggcac tcggacttcg
     5881 agacctggca cgcggaggac gggctgccgc ggatgcggac ggtctccgtg tcgatcgcgc
     5941 tgaccgagaa ccgcgacacc aacggcgccc tgatgatcat gccgggctcg caccggacgt
     6001 tcctgggctg cgcgggcgag acgccgaagg acaactacaa gaagtcgctg cagatgcagg
     6061 acgccggcac cccgtcggac gaggcgctga cgcggttcgc ggacgcgcac ggcatccggc
     6121 tgttcaccgg cgaggccggc tcggcgacgt ggttcgactg caacgcgatg cacggctcgg
     6181 gcgacaacat cacgccctat ccgcgcagca acgtcttcat cgtcttcaac agcgtcgaga
     6241 acacggccgt ggaacccttc gcggcgcccg tccgccggcc ggagttcatc ggggcgaggg
     6301 acttcacgcc ggtccgctga cccgccgcgg ccgccggccg ccgacgccgg ccgccgagac
     6361 gacgaacggg tcgtcagggc ccgcctcccg cacgtgccgg ggaggcgggc cctgacgcgt
     6421 gcgggggcgg gtccggccgg gtaccgcgcc gggaccccgg gtacggggca ggaccggccg
     6481 ggtacggggc agccggccgc ataccgcgct gggaccggtc gtccacggtc cccgggcagc
     6541 gggtccccgc cgtgcgggac gccgggtccg gccgtgcggg cccggcgcgg cgggtcagag
     6601 cgtcgggtag tcggtgtagc cgcggtggtc gccgccgtac agggtggccc ggtcgggctc
     6661 gttgtacggc ccgccggcgg cgagccgggc ggggaggtcc gggttggcca ggaagagcgc
     6721 gccgaaggac aggaggtccg cggtgccgtc ctcgaccagc gtgagcaccc cgtggtcggt
     6781 cgggccgtcg gtcgccgggt tgaggacgag ggtgccggag tactgcttgc gtagctgcgt
     6841 ggtgacgtcc cgcacctcgg gcgacgcctc caggacgtgc aggtaggcca ggcccagcgg
     6901 gtcgagctcg gcgacgagtg ccgtgtacgc gggcagcggg tccggctcgt cgatgtcgtt
     6961 gaaggggttc ccgggcgaga tccgcaacgc ggtccgctcg gctccgacgg cggcggcgac
     7021 ggccttgacc gcctcgacgg cgaaccggac gcggtcgtcc accgacccgc cccactcgtc
     7081 ggtgcgccgg ttggttccgg gcgccaggaa ctggtggatc aggtagccgt tggcgccgtg
     7141 cagctccacg ccgtcgaagc cggcctcgac ggcgttgcgg gcggcggcgg cgaagccctc
     7201 gatcacctgc cgcacctgtg cgccggtcag ctcccggggt tcggggtagt ccttgagccc
     7261 ctcggccgtg tacagctgcc cggcggcggc gacggcggag ggggccacgg ggtggaggtc
     7321 gccgggcagg tcggcgagga cggaggggtg gccgatgcgg cccgcgtgca tgagctgcgc
     7381 gaagatccgg ccgcccgcgg cgtggacggc gtcggtgacc cggcgccagg cggcgacctg
     7441 ctcggcgaag tgcaggccgg gggtgtccgg gtacccctgc ccctccgggg tgggctggat
     7501 cccctcgctg atgatcagcc cggcggaggc gcgctgcgcg tagtactcgg ccatcagctc
     7561 cgtggggacg cggccggggg cgcggctgcg ggtcatgggg gccatcacga cgcggttgcg
     7621 caggggcgtg ccggcgaggt cgacggggtc gaaggcagtg gtcacgaagg gctccagaag
     7681 gctcgggtcg gtcggccgag tagaagaacc gtcgtccgcg cgacggcatt ccttggtcag
     7741 ccaaattctt ccggacgcgg cctagagtgg aaccagtcca cccgaggagc ccccatgccc
     7801 gcccgtcctc cggccgccgc atcccaccgg tcggcaccgg ccgccgcccg ccgggacggc
     7861 acgccgtcgg cggcgcgggg cgggccggtc agcatgacgc tgtcccgggt ggcgcggctg
     7921 caccgggtgg cggcgggccg cttcctgcgg gcggcggggc tctacccggg ccaggagttc
     7981 gtgatgatgc acctgtggga ccacggcccg gtgcgccagt cggagctcat ccgggtgctg
     8041 gatttggacc cgtcgacggt caccaagatg ctccagcgcc tggaacaggg cgggcacgtg
     8101 cgccgcgccc ccgacccggc ggaccggcgg gcggtgctcg tggcggcgac ggacgccggc
     8161 tgcgcgctgc acgcgcaggt cgcggcggcg tggacggagc tggaggacca cagcctcgcc
     8221 gccctgaccc cggacgaacg gcgggaactg gcgcgtctgc tcggcaaggt ggagggcggc
     8281 ctctgcaagc cggcaccgct ctgcccggac ccgcaagggc ccggctcccc cggggccggc
     8341 ccgccccccg agccgaccgc gcagccggcg gatgcggggg cgccgggctc gccgcctgcg
     8401 gacgcggggg ccgcgggacc ggcggcggac gcaaggtagg cgcgtccggg cccggcgggc
     8461 cctcccccgg aggtggcggt cgtcagccgg ccgccacggc gtcgagccct ccgacggcgt
     8521 ccagcgcgtc cagggcccga tccacgtccg cggccgtgtt gtacaggtgg aacgacaccc
     8581 gcaggttccc ggcgcgggcc gaggtgacga cgccctctcg ggccagcgcg ggccggaggt
     8641 ggccgaggcc cgggacggcg acgaccaccg acgcggcggg cacgggggcg tacccgagcg
     8701 cctcggcgcc ctcccggaac cgggcggcga gcgcggtggc gtggcggccg atctcctcca
     8761 ccccgacctc cagcagcacc tccagcgcct gttcggcgcc gtgataggcg aggtaggacg
     8821 gcggctcgtc gaagcgccgc gcgtcggggg cgaggcgctg gacgggcccg tagcagctgc
     8881 cgtaccggtc ctctcccgcg aaccagcccg cgtggatcgg gaccagcgac tcctgcgcct
     8941 cctcggtgac ggtgaggaag gacgcgccgc gcgggcacac gaggtacttg taggcgccgg
     9001 cgacggtgta gtcgtacgcc ccggcgtcca gcggcagcca gcccgccgac tgcgaggcgt
     9061 cgaggagcgt acgggcgccg tgggcggcgg cggccgcgcg gatggcgggc atgtcggcga
     9121 cgcggccgtc ggcggactgc accgaggaga aggcgacgag gtgcgtgtcc tcccgcacgg
     9181 cgccggccag ctcctccagc ggcgccgagc gggtccgcag gtcgccgcgg acgacgaagg
     9241 gcgtcatgac ggagctgaag tcgccctcgg cgtacagcac ttcggccccg ggcggcaacg
     9301 acgccgcgac gagcccgacg tgcgccgaga cggaggcgcc gagcgccact cgccccgggt
     9361 cgaccccggc gagccgggcg aacgcggccc gggcggcggc cacggtgtcg aagctgccga
     9421 caccgtcggg cccacccgcg gcgttctcct ccgccaggcg cttcaccgcc tccaccgcgc
     9481 ggcggggcag cagcccgcag gtggaggtgt tcaggtacgt ctgcgcgggc gcgaactcgt
     9541 cgccccccag gggggtttcc atgacgccac cctggaacac ccccgccccc aagtcaatga
     9601 ccgattccag ccgccttgcc ccaagggatg cttataccgc gcaggtgccg tccgcgccgc
     9661 aggccgccgc gtccccggag gcggtgtcga ccggctgcgg gacccggccc tcccacgcct
     9721 gctccagggc ccgggtgaac acctcggcgg gctggccacc ggagacgccg taccgccggt
     9781 cgagcacgaa gaacggcacg cccgtcgcgc ccagctcggc cgcctcccgc tcgtccgcgc
     9841 gcacgtcgtc ggcgtaggcc gcggcgtcgg cgagcacggc ccgcgcctcg tcctcgtcga
     9901 gccccgcctc gacggcgagc cgcacgagca cctcggtctc gtagacggac cggtcctcgg
     9961 cgaagttggc ccggtaggcc aggtcgagca gcgcctcctg gcggccgcgc gccttggcga
    10021 ggtgcagcag ccggtggacg tcgaaggtgc cgccgtgctc ccgcccctcg acgcggtact
    10081 ccagcccctg gccgcgggcg acggaggcga cgtgctcctc catcgcccgc gcttcctcgc
    10141 gggtccggcc gtacttggcg gcgagcatgt cgacgaccgg cgcgacgtca cccctggcgc
    10201 gccccggatc gagttcgaag gaccggtaca ccacctcgac gtcgtcccgg tgcgcgaacg
    10261 ccgcgaggcc ctcctcgaac cgggccttgc cgatgtagca ccaggggcag gcgatgtcgc
    10321 tccagatctc gacgcgcatg tgtggtcgtc cctccagggt cgtcggcgcg ggcacgcaag
    10381 cggtacggag gccccctcc
//
