LOCUS       NZ_CP101913            10303 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Oceanobacillus oncorhynchi strain QA-1986 526 chromosome, complete
            genome.
ACCESSION   NZ_CP101913
VERSION     NZ_CP101913.1
KEYWORDS    RefSeq.
SOURCE      Oceanobacillus oncorhynchi
  ORGANISM  Oceanobacillus oncorhynchi
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Oceanobacillus.
REFERENCE   1  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 237.47x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024508135.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 08:40:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,482
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 13 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:15:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3554664
            Orig. end                         :: 3564967
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101913.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10303
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10303
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10303
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5303](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5303)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(42..61)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 20.7"
                     /tool="antismash"
     misc_feature    complement(731..751)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.24"
                     /tool="antismash"
     gene            complement(1216..2100)
                     /locus_tag="NP440_RS17545"
                     /old_locus_tag="NP440_17545"
     CDS             complement(1216..2100)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066836.1"
                     /locus_tag="NP440_RS17545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17545"
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="WP_042530402.1"
                     /transl_table=11
                     /translation="MKLVAYRKNKDENYRVGVLTDAESIADVESLSQQFGGKSLPANPS
                     EFYAHASEYIEALQKILPETGRYENLSLQDVQLGMPLGEARKVICVGKNYQEHVHEMKS
                     DIPDFPVLFAKFDNAIIGPEDDIYYPSITEKLDYEAELTIVIGKTASKVKKEDAFDYIA
                     GYTLANDTSARDLQKRTPQWLQGKSLDHTTPVGPWVVTKEEVDNPHELAIRSYVNGEVR
                     QSSNTAHLIFDIPFLINFISEIMTLEPGDIILTGTPHGVGLAMEPSGLLQEGDRVKLEV
                     EGIGTMENQVKKV"
     gene            2242..2745
                     /locus_tag="NP440_RS17550"
                     /old_locus_tag="NP440_17550"
     CDS             2242..2745
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066840.1"
                     /locus_tag="NP440_RS17550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17550"
                     /product="FixH family protein"
                     /protein_id="WP_042530400.1"
                     /transl_table=11
                     /translation="MKKKIFFILIVLMAALAACSNNEADTAEEDDTDSMELPVLEVDFD
                     VPEHVEVGETIDLTAHVTFDDEPVEDAREVLFEVWTQGHADDSVEIEGVHQEDGTYTAS
                     FTFEEEAVYEMYAHTTARELHVMPLKTVIAGDAEPSEEEAHDEMDHEHHEHGHDADEHN
                     QEEE"
     gene            2853..3248
                     /locus_tag="NP440_RS17555"
                     /old_locus_tag="NP440_17555"
     CDS             2853..3248
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017797643.1"
                     /locus_tag="NP440_RS17555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17555"
                     /product="RNase H family protein"
                     /protein_id="WP_042530397.1"
                     /transl_table=11
                     /translation="MIQVYTDGATNVNTEKSGAGIYIKAGEKKLEYTVPLPYMSNHEAE
                     FHAIIEALKICKMNFPEEILSFRTDSKVAADVIDKGRTKNKQFLPLLEIIQAEAADFPF
                     LFIKWIPEKENSRADQLARKAIHQQSS"
     gene            3277..3819
                     /locus_tag="NP440_RS17560"
                     /old_locus_tag="NP440_17560"
     CDS             3277..3819
                     /EC_number="2.7.1.71"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0004765 - shikimate kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155758.1"
                     /locus_tag="NP440_RS17560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17560"
                     /product="shikimate kinase"
                     /protein_id="WP_042530395.1"
                     /transl_table=11
                     /translation="MREIPLKEQSIVLIGFMGVGKTTIGQAVAKKLYRDFIDIDEEIER
                     RFGMPPKEIFAKYGEAVFRQHEKEIISEICHKKLKVISLGGGAFLQKEVQDVCLATTTI
                     FFLDLSWDSWKDRISLLIDSRPVLQGKNMEEIKQLFDDRQPIYGQHHSRVVTDNQEIEE
                     IADYIADSLKLAWELYA"
     gene            complement(3896..4480)
                     /gene="clpP"
                     /locus_tag="NP440_RS17565"
                     /old_locus_tag="NP440_17565"
     CDS             complement(3896..4480)
                     /EC_number="3.4.21.92"
                     /GO_component="GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA]"
                     /GO_function="GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene="clpP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017797649.1"
                     /locus_tag="NP440_RS17565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17565"
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="WP_040978891.1"
                     /transl_table=11
                     /translation="MNLVPTVIEQTNRGERAYDIYSRLLKDRIILLGSGIDDNVANSIV
                     AQLLFLEAEDPGKDISLYINSPGGSITAGMAIFDTMNFIKSDVSTICIGMAASMGAFLL
                     NAGEKGKRFALPNSEIMIHQPLGGTQGQATDIEIHANRIIEIKKRMNRIMSENTGQPLE
                     VIERDTERDKFMSAEQSKEYGIIDDIFERKA"
     tRNA            4751..4826
                     /anticodon=(pos:3559449..3559451,aa:Arg,seq:ccg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="NP440_RS17570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="NP440_17570"
                     /product="tRNA-Arg"
     gene            4751..4826
                     /locus_tag="NP440_RS17570"
                     /old_locus_tag="NP440_17570"
     misc_feature    4787..4800
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: weak, score: 15.81"
                     /tool="antismash"
     gene            complement(5001..5303)
                     /locus_tag="NP440_RS17575"
                     /old_locus_tag="NP440_17575"
     CDS             complement(5001..5303)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_II"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013850.4"
                     /locus_tag="NP440_RS17575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17575"
                     /product="leucocin A/sakacin P family class II bacteriocin"
                     /protein_id="WP_256706332.1"
                     /sec_met_domain="Bacteriocin_II (E-value: 9.4e-15,
                     bitscore: 52.1, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKRMKLGLAVLTLGLSAISTSGVQAASTDYGNGVHCDEKTCSVN
                     WSETFQQSTDRAIESWATGLTSAPAKAKSAIESNYKSSNDSNRPESGTTTGSPSF"
     gene            complement(5712..5969)
                     /locus_tag="NP440_RS17580"
                     /old_locus_tag="NP440_17580"
     CDS             complement(5712..5969)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331997.1"
                     /locus_tag="NP440_RS17580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17580"
                     /product="HPr family phosphocarrier protein"
                     /protein_id="WP_042530267.1"
                     /transl_table=11
                     /translation="MVEKTVKIELDSGLQARPAAYFVQEANRFDSHIFLENDHKKVNAK
                     SIMGLMSLALTKGEEIKLIIEGEDEEEALEYLTSLVREKV"
     gene            complement(6097..7047)
                     /gene="whiA"
                     /locus_tag="NP440_RS17585"
                     /old_locus_tag="NP440_17585"
     CDS             complement(6097..7047)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="whiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019377716.1"
                     /locus_tag="NP440_RS17585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17585"
                     /product="DNA-binding protein WhiA"
                     /protein_id="WP_256704975.1"
                     /transl_table=11
                     /translation="MSFASDIKKELTAIQSDSCCQFAELAALVRMNGVISISKQGYVLD
                     VQTENAAIARRIYTLIKQNYTLPVELLVRKKMKLKKNNVYIVRMKNGVQSLLVDLNLME
                     NQYQIVRTISEKYLRKECCRKAYLRGAFLAGGSMNNPETSSYHLEIFNYYEEHAEALKT
                     LFNAYDLHARTLERKNGYIVYIKEAEKITTFLSVIGAHQALLKFEDVRIVRDMRNSVNR
                     LVNCETANLNKTIGAAFRQIENIKLIERTVGLDQLPEKLQEIAKLRIQYEEVSLKELGE
                     LVTSGTISKSGVNHRLKKIDEFAEKIKRGELQLGN"
     gene            complement(7185..8135)
                     /locus_tag="NP440_RS17590"
                     /old_locus_tag="NP440_17590"
     CDS             complement(7185..8135)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066860.1"
                     /locus_tag="NP440_RS17590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17590"
                     /product="YvcK family protein"
                     /protein_id="WP_042530263.1"
                     /transl_table=11
                     /translation="MSDIPKITAIGGGTGMPVLLRGLKDLPVDLTAVVTVADDGGSTGR
                     IRSEMEMPAPGDIRNVIAALSDAEPMLLELFQHRFQQGNGLTGHSLGNLLLAAMTSMTG
                     NFNQGIKEISRVLNVKGNIYPISNDNMSLHAIMEDGEIIHGESNIPLSGKRIKRVFLEP
                     QPLQPLPNAVESIQKADLIVISPGSLYTSIMPNLIVPEVKEALLNTKAKIVYVCNIMTQ
                     YGETTNYSAADHVQAIHDHVGDDCVDYVIVHDQPIDAETQEIYKEEQAFPVVADSSRLE
                     AMNVQVFPGNIVVKHKDGTLRHNNDKIAKMLYKFL"
     gene            complement(8132..9019)
                     /gene="rapZ"
                     /locus_tag="NP440_RS17595"
                     /old_locus_tag="NP440_17595"
     CDS             complement(8132..9019)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="rapZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009435.1"
                     /locus_tag="NP440_RS17595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17595"
                     /product="RNase adapter RapZ"
                     /protein_id="WP_084612854.1"
                     /transl_table=11
                     /translation="MNQEKETKLVIITGMSGAGKTVAVQSFEDLGYYCVDNLPPALLPK
                     FLELMKDASNTIQKVALVMDLRGREFFDSLYKALDAISHTPWIQSHILFLDAEDETLVT
                     RYKETRRSHPLAVGGLPLNGIHQERIIIDELRGRSQRIIDTSKLKPRDLREKILQLYKE
                     EKQEVFSTHFVSFGFKYGIPIDADLVFDVRFLPNPHYVSALQPLTGLNQEVNSYVFKWS
                     DTKLFVEKLNDLFRFMLPQYKKEGKSQLVIAIGCTGGQHRSVALAEYFSKQFSDDYITH
                     VSHRDIEKRKAKTS"
     misc_feature    9813..9826
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.08"
                     /tool="antismash"
ORIGIN
        1 ttcctccgca attatttgct gtacatattg cttcgcaacc acacgggcga ctcctccccc
       61 tttccgggat tcacgctgca ataaatattt gactcggagg ataccaaaac ttgtctgcag
      121 atatttattg ctgatggtcc ggctgatcgt tgaaacatgc agatccaata catccgctat
      181 ctcctgaagg gttaacggtt ttaatgccgt tatcccttct tcaaaaaagg ataattgttt
      241 attgacaata atatgcaaaa cctgctccaa ggtcttccca cggtattgga tcgcctgctc
      301 cagcatctct atctccttct tcttctcctt caaaaagtct gctccctctt tcggcatatc
      361 agcctgcagg atatcatccc gaagctgaat agtagggctt gaccaattga aaaaggacag
      421 ctgccactct cccttttcct tcaacactgc cgcttccgga ataatatatt ctgtctcttc
      481 ttcctccaat aatttacccg gattcggatg acaaagtttc atattcgaaa ttaatctgcc
      541 agcttcctct tctgtaatcc cgtaggcttc agctaaagcc tgggcatcct cctcggctat
      601 caggaccaaa tcattttcaa aaagctcctc taaaaaggga taatcacaat ccatttcttt
      661 taattgcagc tggatacact cctgtaagct ccgggctcca attcccggcg gctcaaggga
      721 ttgtaaatag gatagagcct gttctatttc ttccatggat aactgacagc cggaagccca
      781 ctcttccata gtaatatcca gatagccatt ttcatttaag gaatcaattc caaaaagaac
      841 cgcctttttt aaattttcct ccgaaataga aagcatgtac acattctctt ttaattgctc
      901 gtacatattt tttttcgaag cattcatatt ttcaaatgcg tttgtctctc catgaaattg
      961 actgagcacc tgctgatcaa tgggacggat atcctcaatc aaaggattct cttcagcaat
     1021 tctttgtaca tgatcatata attcaatacc agagagctgc aggatttcaa tggattgatg
     1081 aagcgattga ttcattttcc aagctaatgc ttgctttaac accagactct gctgcatgct
     1141 ttaacctcca tgcatttcag taggaaatca tttttttcgt attgctgccg ttccctgtat
     1201 taccctatac agatattata ctttcttcac ctggttttcc atagttccaa ttccttctac
     1261 ttctaacttc acccggtctc cttcctgaag caggccggat ggctccattg ctaaaccgac
     1321 accatgcggc gttccggtta aaataatatc tcccggctcc agcgtcatga tttcagaaat
     1381 aaaatttata aggaaaggga tatcaaaaat aagatgcgct gtattagaag actggcgcac
     1441 ctccccattt acataggagc ggatagctaa ttcatgcgga ttatccactt cctcctttgt
     1501 cacaacccat ggccctaccg gcgtggtatg atccagtgat ttcccctgca gccattgcgg
     1561 cgtccgtttt tgcaaatcac gtgcggaggt atcattggca agggtatagc cggcaatata
     1621 atcaaaagca tcttcttttt tcactttaga agctgttttt ccaatgacaa tggttaattc
     1681 tgcttcataa tctaattttt cagttattga aggataataa atatcatcct ccggcccaat
     1741 aatagcatta tcaaatttcg caaataaaac aggaaaatca ggtatatctg atttcatttc
     1801 atgaacatgc tcttgatagt tttttccaac gcaaataacc tttctcgctt ctccgagcgg
     1861 cattcccaat tgcacatcct gaagggaaag gttttcatat ctgcccgttt ctggaagtat
     1921 tttctgcaac gcttctatgt attcggatgc atgtgcataa aattcactag gattcgcagg
     1981 aagtgatttt cctccaaatt gctgcgacag gctttccaca tctgcaatgg attccgcatc
     2041 tgttaaaaca ccaacacgat aattttcatc tttgtttttc cgataagcta ctagtttcat
     2101 gtctattcct ccctttccct cattatagta gagttagagt tggaaaggaa taaatatgtc
     2161 ataatttatt caacctctcc tttggctggt catgctatac taggtgtcgg gagacaataa
     2221 tagagagggg tatacataat aatgaagaaa aaaatatttt ttatcctcat cgttttgatg
     2281 gctgctttag ctgcttgctc aaataatgaa gcggatacag cagaggaaga tgacacggat
     2341 tccatggaac tgcctgttct ggaagtagat ttcgatgtgc cggagcatgt tgaagtcgga
     2401 gaaaccattg atttaacagc tcatgtcacc tttgacgacg agcctgtaga agatgctcgt
     2461 gaagtgttat ttgaggtatg gacacagggg catgcggatg acagtgtgga aatcgaaggc
     2521 gttcatcaag aggacggcac ctatacagcc agctttacct ttgaagaaga agcagtttat
     2581 gaaatgtatg ctcatactac cgcaagggag ctgcatgtca tgccattgaa aacggttatt
     2641 gccggtgatg cagagccatc ggaagaagaa gcccatgatg agatggatca tgagcaccat
     2701 gaacacggtc atgatgcaga cgaacataat caggaagagg aataatttag catcattttt
     2761 caagccattc ctggcccgca cgcattcctt tttgatttaa aattcgttat aatatgaata
     2821 tagaaaagaa cgatttggcg gggggatatg ctttgattca ggtttataca gacggcgcta
     2881 caaacgtgaa tacagaaaaa agcggagccg gcatttatat aaaggctggg gaaaagaagc
     2941 tggaatatac tgtcccctta ccttatatgt ccaatcatga agccgagttt cacgctatca
     3001 tagaagcatt aaaaatttgt aagatgaact tccctgaaga aatcttatct tttcgaactg
     3061 actctaaagt agccgcagac gtgattgata aaggacgaac caaaaacaaa caatttcttc
     3121 ctttgcttga aatcatccag gctgaagcag cagactttcc attcttattt attaaatgga
     3181 taccggaaaa agaaaatagc agagcagacc aattggcaag aaaagcaatc catcagcaat
     3241 catcgtaacg tgctggatga cgagggggaa tgtcatatga gagaaatacc attaaaggaa
     3301 caaagtattg tactaattgg atttatgggt gttggaaaaa caaccattgg tcaagctgtt
     3361 gccaaaaaac tgtatcgtga ttttattgat atcgatgaag aaattgagcg cagattcgga
     3421 atgcctccga aagagatttt tgcgaaatat ggagaagccg tcttccgtca gcatgaaaaa
     3481 gaaattattt ctgaaatatg ccataagaaa ttaaaggtta tctctttagg cggcggcgct
     3541 tttcttcaaa aagaagtaca ggatgtctgt ctcgcaacca ctacaatatt ctttctcgac
     3601 ctgtcttggg acagctggaa ggatcgaatc agcctgctga ttgacagccg ccccgttcta
     3661 caggggaaga atatggagga aatcaaacag ctttttgatg ataggcagcc aatttatgga
     3721 cagcatcact cccgtgtagt gacagataat caagaaattg aagaaattgc agattatatt
     3781 gccgattcct taaaattagc atgggagctt tacgcttaaa aacaagaaat tgcacgtaaa
     3841 aaacaggcta aggtcaccct agcctgtttt tttcgatttc atgatttatt aatgtttacg
     3901 ctttgcgctc aaagatatca tctatgatgc catattcttt agactgttct gcactcatga
     3961 atttatcacg ctctgtatca cgttcaataa cttctaaagg ctgaccagta ttttcagaca
     4021 tgatgcggtt catacgtttt ttaatctcga taattcgatt tgcatgaatc tcaatatctg
     4081 ttgcctgtcc ctgcgtaccg cctaaaggct ggtgaatcat aatctcactg tttggcaatg
     4141 caaaacgttt gcctttctct ccggcattaa gcaggaaagc acccatagaa gctgccatac
     4201 cgatgcagat ggtagataca tctgacttaa taaagttcat ggtatcaaaa atagccatac
     4261 cagctgtgat agaaccacca ggagaattaa tatataaaga gatatctttt cctggatctt
     4321 ctgcttctaa gaataaaagc tgtgctacaa tagagttggc tacattgtca tcaatgccgc
     4381 tgccaagtaa aataatccgg tcttttaata agcgagagta aatatcatat gcgcgttcac
     4441 cacgatttgt ttgttcaata actgtaggta ctaaattcat ttaaaaatcc tccttaagtt
     4501 gccaacgtta atggctgatg attttttcta tactttatat gatagctcat tggtcaatga
     4561 aggtcaaaca aaaagtttca ccaaatttct actttaacca tccttcccga ttttatgttt
     4621 gttaaacatg aaccatttaa acttattgaa agacgcataa aataattttt catctaaatt
     4681 atataaaaag gacttgtctc aaaggaactt cctatgtata attaagattg tgtttttaat
     4741 tcaatgagat gcgcccatag ctcagctgga tagagcactg gtttccggta ccaggtttgc
     4801 cgggggttcg aatccctctg ggcgcgctag ataaagttaa taaaaagaca agggataaag
     4861 atgtttgctt cccttgtctt ttttttctgt cttccagaag tttggtgcta tctttcctca
     4921 aacaccgcca ggcttttcca ctcggttgcg aatgaatact ctattttcct atatgtgata
     4981 ccgtgatacc ccttgcctaa ttaaaaagag ggactgccag ttgtcgtacc tgactctggc
     5041 cggttagaat cattacttga tttatagttg gactcaatag cagatttagc tttagcaggc
     5101 gctgatgtca gtcccgttgc ccagctttct atcgctctat ctgttgattg ttgaaatgtt
     5161 tcgctccagt ttactgaaca agttttttca tcgcaatgaa caccatttcc ataatctgtc
     5221 gaggctgctt gaactcctga ggttgaaata gcggataatc ctaacgttaa gactgctaac
     5281 cctaatttca ttctcttttt catcacaggc actcccttta tataatgatt tgtctctcaa
     5341 cctaacataa cttttgcgaa gattcaacaa gttaccatga agaatggaaa gcaaaatagt
     5401 ccgccgcagc ggcgctttac acttcacctt ctattattta gatagggatt tatccaagat
     5461 acggtcaact cctgtttcta tggaaatgaa gtcacttttt aataacagaa aattaagttt
     5521 atttatattt gaagcagtca aattgtaata cttataggtg caccagaaat tatataatgc
     5581 tatataaata aaagccccct taaaattgag caatgtaact cgcctttaag aaggactttt
     5641 ttagctgggg ttttactatt tagtgctttg tttatctgtt aaataaagca aagttcgtta
     5701 tacggatagc tttaaacttt ttcacgtaca aggcttgtta aatattccag cgcctcttct
     5761 tcatcttctc cttcgataat aagcttgatt tcttctcctt ttgtcagagc caggctcatt
     5821 aatcccataa tgcttttggc attaaccttt ttatgatcat tctctaaaaa tatgtgtgaa
     5881 tcaaatcgat tggcctcttg tacaaaatag gcagctggcc tcgcttgtag tcctgaatct
     5941 aattcgattt tcactgtttt ctcaaccaat aaattcactc tcctgtctta agtaaacgcc
     6001 tccatttttt taggaagcag aaaaaattct gcgtctttct ttcggcgatg ttccatgata
     6061 tttactgcat tatatcatat tcttcggata ctagcattaa ttaccaagct gcagttcacc
     6121 acgtttgatt ttctctgcaa attcatcaat ttttttcaaa cgatgattca cgcctgattt
     6181 agaaatcgtc ccgctggtaa caagttcccc taattctttt aaggaaactt cttcatattg
     6241 aatacggagc ttagctattt cctgtaattt ttccggcagc tgatccaatc caactgtccg
     6301 ttcaatcaat ttaatgtttt caatttgacg gaatgctgcg ccgatggttt tatttaagtt
     6361 cgcggtttca caattcacca gtctattgac agaattacgc atatcacgga caatgcgtac
     6421 atcttcaaat tttaataatg cttgatgtgc tccaatgaca cttaaaaacg tagtaatttt
     6481 ttcggcttct ttgatgtaga cgatataacc atttttgcgc tctaaggttc ttgcatgtaa
     6541 atcatatgca ttaaacaatg tttttaatgc ttctgcatgc tcctcataat aattaaaaat
     6601 ctccaaatga taggaggagg tttccgggtt attcattgac ccgccagcta aaaacgctcc
     6661 gcgtaaatat gcttttcggc agcattcttt tcttaagtat ttctctgaaa tggtccggac
     6721 aatctggtat tgattttcca ttagattcaa atccactaat aaagattgaa caccattttt
     6781 catacggacg atatatacat tatttttctt cagcttcatt tttttacgga caagcagctc
     6841 caccggcaac gtataatttt gtttgattaa tgtgtaaata cgccttgcaa ttgctgcatt
     6901 ctctgtttga acatccagta cataaccttg ctttgatata gaaataacgc cattcattcg
     6961 aactaatgca gctagttcag caaactgaca gcaagaatcc gattgaatag cggttagttc
     7021 tttcttaata tctgatgcga aggacattgt atcacgctcc ttcccctttt cgaacacgcc
     7081 ttgatacgtt tattcaatga aactgtattc agcggaataa tcctgcatac cccgctgaat
     7141 ctgacttcct tgtctttttc atcgtacttt ttgaacactc actactataa aaatttataa
     7201 agcattttag ctattttatc attattatga cggagagttc catctttatg cttcacgaca
     7261 atattaccag gaaaaacctg gacattcatc gcttctaaac gggaactatc tgctactacc
     7321 ggaaacgcct gctcttcttt ataaatctcc tgcgtttctg catcgattgg ctgatcatga
     7381 acaatcacat aatcaacaca atcatcgccg acatgatcat gaatcgcttg gacatgatct
     7441 gctgcagaat aattcgttgt ttctccatat tgtgtcatga tattacaaac gtacacaatc
     7501 tttgcttttg tatttaataa tgcttccttc acttctggaa caattaaatt cggcataatt
     7561 gaggtataaa ggctccctgg agaaattacg attaaatctg ctttttgaat tgattccaca
     7621 gcgttaggta atggctgcag cggctgcggc tctagaaaga cccgtttaat ccgtttgccg
     7681 gacagaggaa tattggattc cccatgaata atctctccat cctccataat cgcatgcaaa
     7741 gacatgttat cgtttgaaat cggatagata tttcctttta cattaagcac ccgggaaatt
     7801 tcttttattc cttgattaaa attaccagtc atggaagtca ttgctgccaa taatagatta
     7861 cctaatgaat gccctgtcaa cccgtttccc tgctgaaacc gatgttgaaa aagctctaaa
     7921 agcatcggct ctgcatcaga cagagctgca atcacattcc ggatatctcc tggtgccggc
     7981 atttccatct cggaacgaat tcttcctgta cttcccccgt catctgctac cgtcacaaca
     8041 gcagtcagat ctacgggcaa atccttcaac ccgcgaagca aaaccggcat tcctgtccct
     8101 ccgccaatcg ctgttatttt cgggatatcg ctcatgaggt tttggccttt ctcttttcaa
     8161 tatctctgtg gctgacatgg gtaatataat catcgctgaa ttgcttggag aagtattcgg
     8221 ctaaagcaac agaacggtgc tgtccgcccg tacagccaat ggcaataact aactgtgact
     8281 tcccctcttt tttatactgc gggagcataa aacggaataa atcatttaat ttctctacaa
     8341 acaatttcgt atctgaccat ttaaacacat aggaatttac ttcctgattt agtccggtca
     8401 atggctgcag tgcagataca taatgcggat tcggcagaaa acgtacatcg aaaactaaat
     8461 ccgcatcaat tggaatgcca tatttaaacc caaaggatac aaaatgagtg gaaaatactt
     8521 cttgtttttc ttccttgtac aattgaagaa tcttttcacg caaatctctc ggcttgagtt
     8581 ttgacgtatc aataatccgc tgggaacggc cgcgcagctc atcgataata attcgctctt
     8641 gatggatgcc gtttaaagga agtccgccga cagctaatgg atgagagcgt ctggtttctt
     8701 tatatcttgt caccagcgtt tcatcctccg catctaaaaa caggatatgg ctttgaatcc
     8761 atggtgtatg gctgattgca tccaatgcct tgtataagga atcaaaaaat tctcttcccc
     8821 gcaggtccat gacgagcgcc actttttgaa tggtgttcga agcatccttc attaattcta
     8881 gaaatttggg aagaagcgca ggaggtaaat tatctacaca ataataacct aaatcttcaa
     8941 agctttgcac tgcaactgtt tttccggctc ctgacatccc cgtaataata acgagctttg
     9001 tttctttttc ctgattcact aaactctccc ctttcagtcc tcttccaaca cacaattgct
     9061 ttttacaggt ataggtactg cagccccaaa gaagttatct gaggttttag cagcccgccc
     9121 cttgttatcg tgcttcttac atcttagttc aattatatta gaaaacctga cattcgccaa
     9181 gcctttgagt gaatgccttc gtttgggctg cgaacaccgg tacgaagata tcccttctct
     9241 tatatagctg acctgtgaaa cttcgccgca gctgagacac tctccctttt tccatttaaa
     9301 caaaaaaatg gacaggtgag tcacctgtcc caaaactaat ctatctatta aaagggggtc
     9361 aattagttaa ctctataata ccctttcatt gtttcatgcg tgttacagca acgttaagat
     9421 gcagttacca tagattaatt tttttataca aatcctaacc aaagatggat atgatttgta
     9481 ttttcgacaa tttccaataa ctatcgtatt atgcatctcc taaaatacga caattttgct
     9541 aattaatctt ctaatgtttc tacatattta attgctgttt ccgctgcgat acttccatcg
     9601 cctgtagctg taacaatttg tctgaggtct tttgcacgga tatctccagc tgcaaaaata
     9661 ccaggtattt tcgtttccat attttcagat gtaggaatat atccttcttc atccgtaatc
     9721 cctaaatccg taaatggatc atttaaagga agcataccga cataaacaaa tacaccgctt
     9781 gttgggaagt catatacttc atccgttttt acatttttta atgaaatgct gctgactttt
     9841 ccctctggac cgttaatttt ttcagctatc gtatcccaaa taaattcaat tttatcgttg
     9901 ttaaatgcgc gatcctgaag aatgcgctgt gcccgcaatt catcacggcg gtgtacaatg
     9961 gttactttgc ttgcgaatct tgtcagatac acgccttctt ctactgcaga gtctcccccg
    10021 ccgacaacaa ccagttcttt ctctttaaag aaagcaccgt cacaaacggc acaataagac
    10081 acaccgcgtc cgccaagctc ttcttcgccc ggaatcccga gtttcttata ttgtgctcca
    10141 gttgcaataa tcaaagcttt agtatgataa actttttttc ctgcataaat ggttttatat
    10201 ttgccatgat cttcaatcga cttaatatca ccgtatgcat attctgcgcc aaacttttta
    10261 gcatgatcga acattttatt cgacaagtcc ggtcctaaaa tat
//
