LOCUS       NZ_CP101913            22313 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Oceanobacillus oncorhynchi strain QA-1986 526 chromosome, complete
            genome.
ACCESSION   NZ_CP101913
VERSION     NZ_CP101913.1
KEYWORDS    RefSeq.
SOURCE      Oceanobacillus oncorhynchi
  ORGANISM  Oceanobacillus oncorhynchi
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Oceanobacillus.
REFERENCE   1  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 237.47x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024508135.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 08:40:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,482
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 13 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:15:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3426711
            Orig. end                         :: 3449024
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101913.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22313
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="8"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..22313
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22313
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:12313](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12313)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    1819..1832
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.45"
                     /tool="antismash"
     misc_feature    1841..1855
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.51"
                     /tool="antismash"
     gene            complement(1953..2261)
                     /locus_tag="NP440_RS16965"
                     /old_locus_tag="NP440_16965"
     CDS             complement(1953..2261)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NP440_RS16965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NP440_16965"
                     /product="hypothetical protein"
                     /protein_id="WP_193063617.1"
                     /transl_table=11
                     /translation="MAGVFSFLSIVLFAFNFWKNRKELRATYSKLHAVQIIGVIISYLV
                     TIAIAFVLIYYAGNWLVSFIPFAFLRSAAFFVTIAVVLFFCLDLLHKVLTRITKGIL"
     gene            complement(2316..3020)
                     /locus_tag="NP440_RS16970"
                     /old_locus_tag="NP440_16970"
     CDS             complement(2316..3020)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NP440_RS16970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NP440_16970"
                     /product="hypothetical protein"
                     /protein_id="WP_193063615.1"
                     /transl_table=11
                     /translation="MKEVIKYSLHDYIRSHKYFPPVSTFIVLIAVFYTYKPNPVVDSYA
                     ITALFLFVVSAWLCMSVLSLDSPVQRQLLVLNLRSSYRYYLSKLAAVWLIALLLAVFAF
                     LYPIIFHLFSDSVSMTVGFVSIVNHLLLAALGICIAAFFSSVMMESAINAYGGLALTVI
                     LSIAALGIENALPASLKYITWIIPPATITQKPLFQWEEENLTYLSGFPFLWIMIYTGII
                     MVLFLIMANRKR"
     gene            complement(3068..3904)
                     /locus_tag="NP440_RS16975"
                     /old_locus_tag="NP440_16975"
     CDS             complement(3068..3904)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="NP440_RS16975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_16975"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_256704944.1"
                     /transl_table=11
                     /translation="MNQLVELKSIKKKYGKKTILYDMSLRLEEREVIAVLGGNGSGKST
                     FLRMAAGVERPDSGQVVYTDKNIRIGYVPERFPNYLRFTPYEYLHYTGRIGDVSEAELK
                     QRISSLLHRFQLDKWSDRRISTLSKGNIQKVGIIQAILPQPELLVLDEPLSGLDFSAQQ
                     ELLLILDELKEQGTAILLTYHEAPIFEEVVDQTYYIKDGQLTEEKLVEKDAVKVIEVKG
                     LTDIDVQEWNELINKERRENSLLLYVNSENSDQLLSRILELQGSIEYVGNIDYKME"
     gene            complement(4151..4480)
                     /locus_tag="NP440_RS16980"
                     /old_locus_tag="NP440_16980"
     CDS             complement(4151..4480)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009334902.1"
                     /locus_tag="NP440_RS16980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_16980"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_212928776.1"
                     /transl_table=11
                     /translation="MIGEKIKQLRQEKRMSLSELAEKAGVAKSYLSSIERDLQSNPSIQ
                     FIEKISQVLEVSVKDLIIENNSIHETELDSEWVNIVKEAMESGVSKEHFREYLEFNKWR
                     QEKGN"
     misc_feature    4493..4507
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.69"
                     /tool="antismash"
     misc_feature    4954..4968
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.11"
                     /tool="antismash"
     gene            complement(5259..6947)
                     /locus_tag="NP440_RS16985"
                     /old_locus_tag="NP440_16985"
     CDS             complement(5259..6947)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709237.1"
                     /locus_tag="NP440_RS16985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_16985"
                     /product="SLC13 family permease"
                     /protein_id="WP_256704945.1"
                     /transl_table=11
                     /translation="MNYVTSAWNELWRQHKRTKSLLNIFALHKNVTEKDVEKHKQTSAK
                     DSTVQGQPEDSGDEPPKKPYSRAQIVGLILGPLLFVLTMLFFQPEGLSAEGKAVLATTL
                     WVATWWITEALPIPATSLLPLILIPITGAMEGGDVASAYGNDIIFLFLGGFFIATAMEK
                     WNLHKRIALFIIAVIGTSVQRILLGFMAATGFLSMWVSNTAAVMMMVPMGLAITVQVAS
                     VLKGTPEEKELPKFEKSLIFGIGYAGTIGGLGTLIGTPPNIILAGQLDELFGVNISFAT
                     WMLFAFPVVVFMLFFTWLYLGKVAFKTSIKQLPGGKELIQRERSKLGKTGYEEKMVAVV
                     FVFAAFMWITRDFLWTDGNFSIVNVPGISDGMIAIVAAVLLFAIPAKKEERILSWKDSR
                     DIPWGVLLLFGGGLAIAAGFTETGLSDWLGEQLTVLDGFHILVVIAAATLMIMALTEIT
                     SNTATATMILPVVGALAVALNIHPFALMIPCALAANCAFMLPVGTPPNAIIFGTGKLKI
                     IEMIKTGFWVNIVGTIIIILAVYYFVPIVWGIDLTSIPSEFLRGN"
     misc_feature    6965..6976
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.12"
                     /tool="antismash"
     misc_feature    7091..7100
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.35"
                     /tool="antismash"
     gene            complement(7359..8060)
                     /locus_tag="NP440_RS16990"
                     /old_locus_tag="NP440_16990"
     CDS             complement(7359..8060)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324351.1"
                     /locus_tag="NP440_RS16990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_16990"
                     /product="response regulator"
                     /protein_id="WP_193063607.1"
                     /transl_table=11
                     /translation="MIRIAIIEDEPLIAKFHQKYIEKIDGFCCTGSVETVEEGIELARR
                     PDVDLILLDIYLQQKNGLDVLKQIRSEEINVDVILITSANDQQSLQTGYRYGVVDYLLK
                     PFTFERFQASLLRYQQTIRVTEGKVHQSEVDRLFHPHEQPVVLKPEDLPKGITKETCRN
                     ILTVMMEHEDWLSAADLSAHANISHVSLRKYLRFFEEKRLLEKNVIYHSFGRPLQQYRL
                     SQTGKNNFRHF"
     gene            complement(8057..9670)
                     /locus_tag="NP440_RS16995"
                     /old_locus_tag="NP440_16995"
     CDS             complement(8057..9670)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /locus_tag="NP440_RS16995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_16995"
                     /product="ATP-binding protein"
                     /protein_id="WP_256704946.1"
                     /transl_table=11
                     /translation="MRKKDMPFMLQMLLFTSGLICLTLLVTGLLIGYNQATGTKEALAD
                     KASLSASHFARMPIVADALAEGEADDELRTIAAEVRENNNLQYIVLMNMEGIRLMHPVP
                     ERIGEHFVGGDIEDVFEGKSYTSEAVGTLGPSMRAFEPVYDQDGMQIGAISVGISTEVI
                     NQAVWDSVKITIYGTAVSLLLGLAGSYFLARRLKKTLFNLEPQEIAYLIEEREAMLDSV
                     SEGVIAINADGYIIIANPSAEIMLERAGLHGNIIGQKISEIWPELSLEVFVNGEKAVYD
                     KAVRFGELEMITNSVSFQVDKHNVGALVTFRDRNELDAMMKKLSGVESYAHTLRMQTHE
                     FMNKLHIIGAMVYTESYEELAEYVDSLSMIYQRETGIISAHIDDVAIAGYLLTKLERLE
                     QLQIDVDIQGENKWPSLSDPTAVDRWITIIGNSLENAVEAMVGQEEKKVTLIFDVFDGA
                     LFYEIRDNGEGFDVEDLPFILEKGYSTKGLNRGYGMTIMINAVIAGNGNYHIQSEIGAG
                     VCLQITMPYEWNDKEGNNSQ"
     gene            complement(10001..12313)
                     /locus_tag="NP440_RS17000"
                     /old_locus_tag="NP440_17000"
     CDS             complement(10001..12313)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018932486.1"
                     /locus_tag="NP440_RS17000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17000"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_256704947.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 3.1e-42, bitscore:
                     142.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQMDKDYFQTKQEQAKAYFQTLQAQIQEENFASALIETLHQYQQD
                     KKKTWVNIDHWKQQPSKLFISYLAKSGQLDAYLHRSISYIYFRDLGRDITSEAMQNRIT
                     AVTEGVKNYLNQKEQRVPFHLTELYQMAQKESLEEVFFWVSDKLKRVSAILPDGLDKNE
                     AKRKIMKIIAGVVMHELEELDGELSKDRRRQLLDQAVRTGYYYGLTYPLIDDVLDADML
                     TASEKKTFAYFIRQTLFTGEVLPVKADDWQKNHWEFISAVHQELSEAFHYLKSCQPEEN
                     RQLFLENAYLFFEAQELDRQKHLDNDRYTNEDLYTSVILKSASSRLIIRSFKPFSEVDN
                     IEANMFHYGMYNQLADDLTDLDEDLANRNVTPYTYFVTYREQNSNLTNPFAMYWSVIYY
                     FIHKLYPGKPEIRDVILDRAINGLKRLKARLGKERYQKLMQQFSFDPNFDKALEKTVHR
                     THNVAFFDKWIRDQFLDTLKQNQQLKNQFVSKMKEIKEQVEEQLPFESAKNSLEEAAND
                     SLSGNAKRLRPMMTWVLAKEGLRLPDHLVLPLVKSIEYMHTASLIFDDLPSQDNAPERR
                     GKQTLHEKYNHATAELTGLWLTQRAVEEQAVIKHQEPGRLLELIAYSTRTTQNMCQGQF
                     LDLQAKGKQLTLDELRELSVYKTSFGFEAALMMPMIMAGESDERKQAVQRFAYHAGIAF
                     QIKDDLLDITGDAKLLGKQPGRDERNNSSTFVSILGKEEAQKEMWRHYMAARKELATLP
                     DIAFFYQLLDYLILRDH"
     misc_feature    complement(12389..12406)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 20.02"
                     /tool="antismash"
     gene            complement(12540..15176)
                     /locus_tag="NP440_RS17005"
                     /old_locus_tag="NP440_17005"
     CDS             complement(12540..15176)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813165.1"
                     /locus_tag="NP440_RS17005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17005"
                     /product="cation-translocating P-type ATPase"
                     /protein_id="WP_193063602.1"
                     /transl_table=11
                     /translation="MEMYQKKAADVLEELEASQQGLSTEEAASRLDQYGYNEIADKEKV
                     PTWKLFLETFKDPMVIVLLAAAAVQILIGEWVESIIIFLVLLINSVISVVQTRKAESSL
                     EALRDMSAPDANVMRDGTEKTIAAKELVPGDIVYLEAGDYIPADGRMLESGSLRVNEGM
                     LTGESEAVEKNTDIIEDEAALGDRLNMVYSSSLVVYGRGTFVVTGTGNQTEVGKIADML
                     STAEQKETPLQQKLNSFSKKLGVGILILCIAIFAVQAARIWFGDSEADTTTALLNALMF
                     SVAIAVAAIPEALQSIVTIVLSVGTNKMAKQHAIIRKLPAVETLGSTRIICTDKTGTLT
                     QNKMTVTDDFIPFHKNERLPENPDEWDKSERFLVYIAALCNDSYINQENQEIGDPTEVA
                     LIQFADKHAHSYQELRKKYPREAELPFDSDRKLMSTVHHMDGERLLFAKGGPDVMFHRA
                     SHVLLDGEEQPLTDDLLERFEGANEDFSNQALRVLAYGYKRLPDNQTDITYEDERDFVL
                     VGLTAMMDPPREAVYGSIEEAKAAGIRTIMITGDHKTTAQAIGRDIGLMENDDIAITGK
                     DLDKMSEEELEDKIEQISVYARVSPENKIRIVRAWQQRGAVTAMTGDGVNDAPALKQAD
                     IGIAMGSGTDVSKDSSAMILTDDNFVSIVNAVQVGRTVFDNIKKAIGYLFAGNLGAILA
                     ILYAVIFNLPNPFTALQLLFINLVNDSLPAIALGVEKSEAGVMKRKPRPVNEGIFAGGT
                     LRTVLTRGILIAAAVVISFYIGLSHSNEMGVAMAFTTLIMSRTLQTFAARSNTQTSIKI
                     GFFSNKYVIGAVVIGFALYGITLLPFAREVFNIPASFGFQEWGIATGLAAAAVVCMEIA
                     KKIFHRA"
     misc_feature    15455..15468
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            complement(15564..16028)
                     /gene="nrdG"
                     /locus_tag="NP440_RS17010"
                     /old_locus_tag="NP440_17010"
     CDS             complement(15564..16028)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /GO_process="GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="nrdG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009329697.1"
                     /locus_tag="NP440_RS17010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17010"
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="WP_226894831.1"
                     /sec_met_domain="QueE (E-value: 1.2e-11, bitscore: 42.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.8e-36, bitscore:
                     123.5, seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMETARILSIYHDSVVDGEGLRTVIFFAGCPHYCKGCHNPRSWNI
                     RNGKEMDVQQIVEEAWNPLTDITLSGGDPFYQAQAAASIARQLKALGKNIWAYTGWTIE
                     ELRENKDPHQLELLNYVDVLVDGPFILAERDLTLSFRGSRNQRIIEMGNE"
     gene            complement(16025..17902)
                     /locus_tag="NP440_RS17015"
                     /old_locus_tag="NP440_17015"
     CDS             complement(16025..17902)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094695.1"
                     /locus_tag="NP440_RS17015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17015"
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="WP_226894830.1"
                     /transl_table=11
                     /translation="MAEVMTAPESTEADLMQDFQEIVSSTNQDLIQENANVDGKSPCGQ
                     MNKFASESAKLYAKEKMLSREAKQAMEDNYIHIHDLDYMPTGTATCCQIPLGKLLEKGF
                     NTGHGHMRSPQSILSAMALAAIVFQSNQNQQHGGQSFQAFDYDLAPFVKKSYQKRLHRL
                     QQYQLPLAPQELEKLAWEETDRECYQACEAFIHNLNSLHSRSGGQVPFTSINYGTDTSR
                     EGRLIIKNLLLATQAGLGKGETPIFPIQIFKMKKDVNFYKADPNYDLYQLALETTAKRL
                     FPNFSFIDAPFNFVYYEEGNPDSEVAYMGCRTRIMANRHGEENSLGRGNISFTSINLVK
                     IALTSSTVEAFMAKLEVYTEMVIRQLYERYVFQANKLVNNFTFLHGEGVWTGSDELNPE
                     DTLEEVLKQGSLSVGFIGLAECLKALTGKHHGESEQAQELGEEIVAFLRKKCDEAAEDY
                     DLNYSLIATPAEGLSGRFVKQDCKDFGKRDGITDRDYYTNSFHIPVYYPIRAVEKIKRE
                     AVYHPYTNAGHITYIEVDGDVSKNIKAIDTLVKTMAESGIGYGSVNHPVDRCQSCGYTG
                     TIDNECPSCQIADERQFDRIRRITGYLVGTMDKWNTAKASEERDRVRHV"
     gene            complement(18096..18710)
                     /locus_tag="NP440_RS17020"
                     /old_locus_tag="NP440_17020"
     CDS             complement(18096..18710)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /locus_tag="NP440_RS17020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17020"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_212928773.1"
                     /transl_table=11
                     /translation="MRMPLFLEVKNQTVIAVGGGAVAARKIRHFLDAGAAITIIAPKLH
                     PFLQQLYQKGKIKWQEREVHEGEHFNCLFLLLMTDKKELNVSLYENKQPHQLAYVANDA
                     MKSDVSFPAVLQRGALSLALSTGGASPIYAKQLKNKLADQLPAEIEADLIFLDQVRKRI
                     LTLPLTPEQKKQILQKITEEEFLRQGNRQQLLEEMLETVQK"
     gene            complement(18710..19486)
                     /locus_tag="NP440_RS17025"
                     /old_locus_tag="NP440_17025"
     CDS             complement(18710..19486)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728231.1"
                     /locus_tag="NP440_RS17025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17025"
                     /product="sirohydrochlorin chelatase"
                     /protein_id="WP_256704948.1"
                     /transl_table=11
                     /translation="MQAILYIGHGSRVQRGNEELLAFVDRAKKKNPQIQIQETCFIELA
                     SPSIQTGIDSCVRQGAAQIAVVPVLLLSAGHAKQDIPKEIGQAEKRYPDVTFTYGKVMG
                     VDPAIIDLLNKRLEMGGLKQTGKPPVYEEREPASVLLVGRGSSDPDANSDLAKIARLLW
                     EAAPVNEVNTCYLAATRPTLDEGLEQLLKSPNSVVYVVPYLLFSGVLMRELRQKLDELS
                     ERTDKQFILCPYLGFDDQLVTVLVDRVNTLLKGRVS"
     gene            complement(19571..21061)
                     /gene="cobA"
                     /locus_tag="NP440_RS17030"
                     /old_locus_tag="NP440_17030"
     CDS             complement(19571..21061)
                     /EC_number="2.1.1.107"
                     /GO_function="GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cobA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958294.1"
                     /locus_tag="NP440_RS17030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_17030"
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="WP_226894829.1"
                     /sec_met_domain="TP_methylase (E-value: 6.5e-44, bitscore:
                     148.4, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMTKPGCVYLVGAGPGDIGLMTLKGYTYLQQAEVVLYDRLVNPLL
                     LEYASPQAEFIYCGKMPKKHILRQEAINELLVKHGLEGKNVVRLKGGDPSVFGRVGEET
                     AALEQAGVPYEIVPGITAGIGAAAYAGVPVTHREHSLSFAVITGHDKSPSGEPLIDWHA
                     LANGIDTIAFYMGLGNLPYISEQLISYGRSSDTPVLLIQWGTTGKQRTLQGNLATIVQK
                     AKAAQFKNPAIILVGDVSTLKKGESWFESKPLFSQHLLLGRAGEEPSEMARHLREQGAE
                     VFEFPRMQKKKHLLKKADIPANRDLLFLSPASVDIFFESLQANKIDIRRIQGEFYGISK
                     KTVKAIESYHCFGKEIAERSPDASPLVILGDPEGFHRKEQRYPFVFEPDFIATHSFIRV
                     QQTEQTFSRLQEEERIETIIFPNARSVKTVTEGFQACGETPENISTYARVICFGEKSKQ
                     AAVKAGYAVSEVLSEPTEEALQEVLLQKEAPLAAAAFS"
ORIGIN
        1 catgtggata tatccaaaaa tcaatccgat gaggagaaaa accaccgtcc ccactgcaat
       61 gatgatattt cccttcatat tggctgttgt tttctttgtt aaaccaaaag cgtggatcaa
      121 ttttttaaaa tgagagtgca ttaggataat atcagaggtt tccactgcta tatccgtccc
      181 gcttcccatt gcaattcctc catcagctaa agcaatagaa gggctgtcat tcactccatc
      241 tccgataaaa gctaccgttc ttccttcttc ttgaagccgt tttataaagc tggacttatc
      301 ctctggaagg aaatccccat gaacctccgt tatacccaat tcatttccca ctatctctgc
      361 cgttgtctga ttatctcctg tcaacatcac aaaacgcttg atccccattt gttttaaaat
      421 acgcaacgtt ctttttgcat ctggccgaac ctggtctttg ataccaatca gcagtgttaa
      481 ttgacgattg attgctacaa atacggtaga attccctgac tgttgaagat aaagtaaatc
      541 ctgttttgcc tgattattta tccaaacagc atgattctcc atgaaggtct gattaccaat
      601 cattatttcc tccgaaccga cgtgagcaat aattccttgt cctttcacaa cctctgtttt
      661 atcaattttt gcatattcgg ttttccccac ataatctaag attgcttgcc ccaatggatg
      721 ctctgattct ctctccacac tcgcagctaa ccgaagtgct tcttgaaaat catccgtata
      781 tgctttcgct tcagaaactg tcggtttgcc actcgttaat gttccagttt tatcaaatac
      841 catcgtatct gccttgctga atgcagccat caattccccg ctcttgatta gaatgccatt
      901 ttttgctcca ttgccaattc cggcaacatt agagaccggg acaccaataa ccagcgcccc
      961 cggacatcct aaaattaaaa tcgtaattgc cagatggaca ttctgaaaaa tcaaaccgac
     1021 aataagcgca agcagcaaca caatcggcgt ataatatttg gcaaaccgat ccataaaacg
     1081 ttccgcttcg gatttattat cctgtgcttc ttccacaagt tcaataattt ttccaaaggt
     1141 cgtgtcttct ccaattttct ccgcctctat cttcaacgta ccattttcta aaattgtacc
     1201 ggcaaacact ttctctccgg ttgtttttcc tgctgtcatg gattcacctg tcacacttgc
     1261 ttcattgata tagccttttc cttcttgaat aataccatcc acaggaattt gcgctcctgt
     1321 cttaacaagg agcaaatccc ctttcgttac gtcatcaata ttaatttctt ctatattatc
     1381 accgttgatt cgtaaagcgc tggcgggagc cattttggta agttcccgaa tagcagagcg
     1441 tgttttttcc aatgtttttt gttccaataa ccctccgaac aaaaacagga aaataacaat
     1501 agcagcttca ctgaattcac cgatgataaa cgctccagta acagcaatgg ataccaacag
     1561 ttcaatactg attactttta ctttcagtgc ttggtacgct tggataaata tcggcggaat
     1621 cccgataatt gcagccattg caagtgaata taccggattc caatcaaata aaaattttga
     1681 aataaatgct gtaataatca atattccgca gattagtaca acgggatgct tatgtcccat
     1741 aaagaaattc tgcagcttca ttttctctcc ccctctccga ttacactgtc attttatcgt
     1801 gaaagaaaga gaaaataatt gatgcttatc aagataggaa aactcatttt gcaaaacggt
     1861 tatttgtaca aataaaaaaa gcatcctcga aagatacttt ctttacatat accgaaataa
     1921 atcccaaaca gtgggctatt ttgaacataa acctacaaaa ttccttttgt tatcctcgtt
     1981 aatactttat gaagcaaatc taaacaaaaa aagaggacga cagctatcgt tacaaaaaat
     2041 gcagccgaac ggagaaatgc aaatggaata aagctgacca gccagtttcc agcatagtat
     2101 attagcacaa atgctatcgc aatcgttacc aagtaggaga taataacacc gattatttga
     2161 acagcatgaa gttttgaata ggttgctctc agctcttttc tgtttttcca aaaattaaaa
     2221 gcgaatagaa ctatactcaa aaaagaaaat acacctgcca agattctcct cctttacttt
     2281 tatattgtaa atctcttatt gctttttcgt agtaatcaac gttttctatt tgccattatt
     2341 agaaatagca ccataataat cccagtataa atcattatcc ataaaaatgg aaatcccgat
     2401 aaataggtca agttttcttc ctcccattga aatagcggct tctgggtaat cgtggccggt
     2461 ggaataatcc atgtaatata tttgagagat gccggtaaag cattctcgat acctagtgca
     2521 gcaatggaaa gaatgaccgt cagtgctaaa ccgccatagg cattgatggc actttccatc
     2581 attacgctac tgaaaaaagc ggcaatacaa attccgagcg cagccaatag gagatgattc
     2641 actatcgata cgaagccaac tgtcatggag acagaatcag aaaatagatg aaatataatc
     2701 ggataaagaa aggcaaacac tgccaatagc aaggcaatca gccaaaccgc tgctaatttt
     2761 gacaagtagt atcgatagct gcttctcaga tttaaaacca gcagctgtct ttgtactggt
     2821 gaatccagag acaagacgct catacataac caggcagaga ccacaaacaa aaacaatgct
     2881 gtaatcgcat agctatcgac aaccggattc ggcttatacg tataaaaaac agcgattaac
     2941 acaataaagg tggagaccgg cggaaaatat ttatgagaac ggatataatc atgcaagctg
     3001 tatttaatga cttctttcaa ataaatctct ccttctatgt aaaacgaaca aacacatagt
     3061 ttgttcttca ctccatttta taatcgatgt ttccgacgta ctcaatgctt ccctgcaatt
     3121 caagaatcct ggacaggagc tgatcgctat tttctgaatt cacatatagc agcaagctat
     3181 tttctcttct ttctttattt attaattcgt tccattcctg cacatctata tccgttaaac
     3241 cctttacttc aatcaccttc acagcatctt tttccacaag tttttcttct gttaattgcc
     3301 catcttttat gtaataggtt tggtcaacaa cctcttcaaa aatgggagct tcatgatagg
     3361 ttaatagaat ggcggttcct tgttccttta attcgtctag aatcaaaagt aattcctgct
     3421 gcgcggaaaa atcgagcccg gataacggtt catctaaaac taacagctct ggctgcggaa
     3481 gaatggcttg aataatacct accttttgga tattcccttt agacaacgtg ctaattcgcc
     3541 ggtcactcca tttatccaat tgaaaccgat gcaataacga cgaaatacgc tgtttgagct
     3601 cagcctctga aacatcgcct atccttccgg tatagtgcaa atattcatat ggtgtaaaac
     3661 ggagatagtt tggaaacctt tctggtacat accctatccg gatattctta tcagtatata
     3721 ctacctgccc tgaatccggt cgttctactc ctgctgccat acgtaaaaag gtgctctttc
     3781 cactgccatt cccacctaag acagcaatga cctccctttc ttccaagcga agtgacatgt
     3841 cgtataaaat agttttcttc ccgtattttt tcttgatact ctttagctct acaagctgat
     3901 tcatgtaatt ctccttgtca tatcatttgg tatatactga catactcaat tctgcctttt
     3961 tacagttcct tcgctaaatg atctatgttc aaaggatatt attatagcta tttatcatat
     4021 tatatatatt tatcttacaa ttaaatataa atgataaata gaaacttcat taaagccagt
     4081 tacactgcga aaaattgatt ataaaataac tctcgatgat ttccataaag ctgctgaacg
     4141 atttactgac ttaattccct ttttcctgcc tccatttatt aaattctaga tattctctaa
     4201 aatgctcttt cgacacacca gattccattg cttcttttac aatattcacc cattcactat
     4261 ctaattctgt ttcatgtatc gagttgtttt caattattaa atctttcaca gaaacctcta
     4321 acacttggct gattttctcg atgaattgga tggaggggtt tgattgaagg tctcgctcga
     4381 tggaacttaa ataggatttt gctactcctg ccttttcagc cagttctgat agcgacattc
     4441 gcttttcttg acgtaattgt ttgatttttt caccaatcaa gttattcacc tgctttttct
     4501 gaaaattata tcataatatt gctggggagt tctattatta aaaattaaca gtttgttctc
     4561 agatagaggc taaatataat cagaagaaga ataaattaga tgaacaatcg ttccgttttt
     4621 aaaacaattt aagttattta gataaccgat atgattaatt catttaaaag gatttatttt
     4681 tacatataat ctttccatat aaatctgtcg aaaaaactca agtaacccac tgcggattat
     4741 acaatgtctc caagctttgt gttgtttatc tttattctct gtaatagact tcaagtgtcc
     4801 tgttagttgg gttgcattac ttgaagtgaa gactcttgct gatttttcat ttccgttatc
     4861 gtcatgattt ctctttttat tttacagaat tgaaatgaaa atatacaact catccgttca
     4921 ttctcttatg aaaacgcata gcctgcagtt acattttttc tgatatttgg ctcatactaa
     4981 ctaaaaagga ggtattggaa tgagcataga taaaggaaaa aagaagaaga aaaagaataa
     5041 agaagaatta aatgccatga agggcgggaa agaaaggcat cgcagaaatc gttcttaggt
     5101 tggtgtataa gaggaaggct gaatcaaagt tatttcatcg ctgagactcc atcatataac
     5161 gaaagcctcc ctgaattgaa ttccagggag gctttcgtta tgaactattt acaagtaaaa
     5221 atcgaataca taaataccag ccagctgcct aattccaatc aattccctct caagaattca
     5281 cttggaatgg aggttaaatc aattccccaa actattggaa caaaataata cacagctaaa
     5341 ataataataa ttgttcctac gatattgacc cagaatcctg ttttgatcat ctcaataatt
     5401 ttcagttttc ctgtaccgaa aataatggca ttcggcggtg ttccaaccgg tagcatgaat
     5461 gcacagtttg ctgccagtgc acatggaatc atcagcgcaa acggatgaat gtttaacgca
     5521 accgccaatg ctccaactac cggcaaaatc atggtcgctg ttgctgtatt ggaagtaatc
     5581 tccgtcagag ccataatcat caaggttgct gcagcaataa ccacaagaat atggaagccg
     5641 tctaaaacag tcagctgctc cccgagccag tcagataagc ctgtttctgt aaaaccagcc
     5701 gcaattgcca atccgcctcc aaacagcagg agcacccccc atggaatatc acgggaatcc
     5761 ttccaagata aaatacgttc ctcttttttt gctggaatag caaataataa tactgcggct
     5821 acaattgcaa tcatcccatc agaaatccct ggaacgttaa caatgctaaa atttccatct
     5881 gtccagagga aatctcttgt aatccacata aatgcagcaa aaacaaacac tactgcgacc
     5941 atcttctctt catagcctgt tttccccagt ttacttcgtt cacgctggat taattctttt
     6001 ccgccaggca gctgcttaat acttgtttta aatgcaacct tccctaaata taaccatgta
     6061 aagaaaagca taaaaacaac aacgggaaaa gcaaacagca tccaggtcgc aaaagaaata
     6121 tttacaccaa ataattcgtc cagctgccct gccagaataa tattcggagg tgttccaatc
     6181 aatgttccca aaccgccgat tgttccagcg taaccaatcc caaaaatcag ggatttttca
     6241 aactttggta attctttttc ttccggagtt ccttttaaca cacttgctac ctgtactgta
     6301 atcgccagcc ccattggaac catcatcatt accgcagccg tattggaaac ccacatagaa
     6361 agaaaaccgg tagctgccat gaaaccaagt agaattcgct gtacacttgt tccaattacc
     6421 gcaatgataa aaagggcaat acgcttatgc agattccact tttccatggc tgtggcaata
     6481 aaaaatccgc ctaaaaatag aaaaataata tcatttccat aggcagaagc aacatctccg
     6541 ccttccatcg ctcccgttat cgggattaat atcaatggca ggagagacgt agccgggata
     6601 ggcaatgcct ctgtgatcca ccatgtggct acccataatg tcgtagccag aacagccttt
     6661 ccttcggctg ataatccttc cggctgaaaa aataacatcg ttaaaacgaa taaaagcggt
     6721 cctaaaatta aaccgacgat ttgtgccctg gaataaggtt tctttggcgg ttcgtctcct
     6781 gaatcttcag gctgcccctg aaccgtgctg tcctttgcag aagtttgttt atgtttttca
     6841 acatcttttt cagtaacatt tttatggaga gcaaaaatat ttaatagact ttttgtgcgt
     6901 ttatgctgcc tccaaagttc gttccatgcc gatgttacat agttcatcgt tcatacccct
     6961 ctctgaaaac gcttaccaaa aatggttatt cttattttcg tctatttact atggtttgta
     7021 tatatttttt aactaatcga aactagaaat acgtgttcaa aaagttcgat aaataggacc
     7081 agccagctaa gaacgcgacg tcctgtcgca acgccgacac tagcacatcc tgtgcgtcga
     7141 aagttcaagg cgacgagcta tttacgaacg gactttcaca ggatgtgatg acttctgtgt
     7201 tgtccacagg acgtggacgt tcttaacaga agttctttcg tgctttataa tacgtgagta
     7261 acggtcttgg tggggcgagt aatcgcagac cccatcggaa atagctcgtc aacgtagaaa
     7321 ttcgccgtgt catttttatt ggactttttg aacatcctct agaaatgacg aaaattgttt
     7381 tttcctgttt gcgagagtct atactgctgc agcggacgtc cgaagctatg gtaaatcaca
     7441 tttttttcaa gcaatctttt ttcctcaaaa aaacgtaaat atttacgcag ggatacatgg
     7501 gaaatgtttg catgagcact taaatctgcc gctgacagcc aatcctcatg ctccatcatg
     7561 accgttaata tatttcgaca tgtttctttc gtgattcctt ttggcagatc ctccggtttt
     7621 aacacaacag gctgttcatg cgggtgaaat aaacgatcca cttctgattg atgcaccttt
     7681 ccttccgtca ctcttatggt ttgctgataa cgtaggaggg acgcctggaa acgttcgaag
     7741 gtaaatggct tcagcagata atctacgact ccgtagcggt aaccagtctg taacgattgc
     7801 tgatcattgg cggatgtgat taaaataacg tccacattta tttcttctga acggatttgt
     7861 tttaatacat ccagcccatt tttctgctgt aaataaatat cgagtaaaat caaatcaaca
     7921 tctgggcgcc ttgccagctc aataccctcc tcgaccgttt ctacactccc tgtacagcaa
     7981 aatccatcaa ttttttcaat atacttctga tgaaatttag caataagcgg ttcatcttca
     8041 ataatagcta tccgtatcat tgtgaattat tcccctcttt gtcgttccat tcatatggca
     8101 tcgtaatttg taaacaaacg cctgcaccaa tctcagattg aatatgataa tttccattgc
     8161 ctgctataac tgcattaatc ataatcgtca tcccgtagcc gcggttcaat ccttttgtcg
     8221 aatagccttt ttccaggata aaaggtaaat cctcgacatc aaatccttct ccattatcac
     8281 ggatttcata gaataacgct ccgtcaaaaa catcaaaaat caatgtgacc tttttttctt
     8341 cctgaccgac catcgcctcg actgcatttt ccaggctgtt tccaataatc gttatccagc
     8401 ggtcaacagc tgttggatca gaaaggctcg gccatttatt ttccccttgt atatccacat
     8461 caatttgaag ctgttccagg cgctccaatt tggtcaataa atatcctgca atggctacat
     8521 catcaatatg agcggaaata atccctgttt cccgctgata gatcatggaa aggctatcta
     8581 catattccgc gagctcttca taggattcgg tatagaccat tgctccgata atatgaagct
     8641 tattcataaa ctcatgcgtc tgcatccgta acgtatgggc ataggactct actccagaaa
     8701 gcttcttcat catggcgtcc agctcatttc gatcccggaa agtgacaagt gccccgacat
     8761 tatgtttatc cacttgaaag gaaacactgt tcgtgatcat ttctaactca ccgaagcgga
     8821 cagctttatc ataaacggcc ttttccccgt ttacgaatac ttccagtgac agttcaggcc
     8881 agatctcact tattttttgt ccaataatat tcccatgaag cccggcgcgc tccagcatta
     8941 tttccgcgga tggattcgca ataatgatgt aaccgtctgc attaatagcg ataactcctt
     9001 cacttacgct gtcaagcatt gcttcccgtt cctctatcag ataggcaatc tcctgaggct
     9061 ccaggttaaa taaggttttc ttcagcctcc ttgctaaaaa gtaagagcct gctaaaccga
     9121 gaagaagact tacagctgtt ccgtaaatgg ttatctttac actatcccat acagcctgat
     9181 taataacctc tgtggaaatc cctacagaaa tcgctccgat ctgcatacca tcttgatcat
     9241 aaaccggttc aaaagcgcgc atcgacggtc ctaaagtgcc aactgcctca gacgtatagc
     9301 tttttccttc aaacacatcc tcaatatccc cgccgacaaa atgttctcca atccgctccg
     9361 gaacaggatg catcagccgg atcccttcca tattcatcaa cacaatatat tgcagattat
     9421 tattctcgcg aacctcggct gcaatagtgc ggagctcgtc atctgcctcc ccttctgcaa
     9481 gcgcatccgc aacgataggc attcgggcaa aatgacttgc actcaagctc gctttatcag
     9541 caagcgcttc ctttgttcct gttgcttgat tatatccaat aagcagtcct gtaacaagca
     9601 gcgtcaggca gattaatccg ctggtgaaca gcagcatttg aagcatgaag ggcatgtctt
     9661 ttttccgcat accagctcgt cctcctccct gttcagtcaa actgcttctt tttttacaat
     9721 tgtataattt ttattcatat caaggagtgc agctttgcca gcacactcca taactgtccg
     9781 tatattttaa aataataaca cagattccgt agctatataa gttgaaataa agattttagc
     9841 ttctagtaat cactccaact ttttatatac tccctctttt tactaacatt tttacctgtt
     9901 ttctttctta tccctaaaaa aatccagata accttcacat acaataaagc gtatgtaaag
     9961 accatccaga tttacaaaat cgatagtaat atgaacaaac tcaatggtca cgcaaaatca
    10021 aataatctaa cagctgataa aagaaagcaa tatccggcag cgttgctaac tcttttctgg
    10081 ctgccatata atgtctccac atttccttct gcgcctcttc cttgccaagg atggacacaa
    10141 aggtcgagct gttattccgc tcgtctcttc ccggctgttt gcccaacagc ttcgcatccc
    10201 ctgttatatc taataaatca tctttgattt gaaaggcaat gcctgcgtgg tacgcgaaac
    10261 gctggactgc ctgtttccgc tcgtccgatt ctccagccat aatcatcggc atcatcaggg
    10321 ccgcttcaaa accaaaggag gtcttataaa ccgataattc tctcagctca tccagcgtta
    10381 attgctttcc ttttgcctgc agatccaaaa attgtccctg acacatgttt tgggtggtcc
    10441 gggtggaata agcaataagc tccagcagcc tgccaggttc ttgatgtttg attacagcct
    10501 gctcttctac agcgcgctgt gttagccaaa gtccagtcag ctccgccgtc gcatgattat
    10561 atttctcatg cagcgtctgc ttgcctcttc tttcgggtgc attatcctgt gacggcaaat
    10621 catcaaaaat aagcgaggcg gtatgcatat attctattga ttttactaag ggcaggacca
    10681 aatggtctgg aagacgaagc ccttcttttg ccagaaccca cgtcatcatc gggcgtaaac
    10741 gcttggcatt gcctgacagg ctgtcgtttg ccgcttcttc taatgaattt ttcgctgact
    10801 caaagggaag ctgttcttct acttgttctt tgatttcttt cattttcgat acaaactggt
    10861 tctttaattg ctgattttgt tttaatgtat ctaaaaattg atcacggatc catttatcaa
    10921 agaatgctac gttgtgcgta cgatgtactg ttttctctaa agctttgtca aaattcggat
    10981 caaaggaaaa ctgctgcatc aatttctgat agcgttcttt tcccaatctt gcctttagcc
    11041 gttttaaacc gtttattgcc ctgtccaaaa taacatctct tatctccggc tttcccggat
    11101 agagcttatg aataaaataa taaataacag accaatacat ggcaaaggga ttggttaaat
    11161 tagaattctg ttcacgatac gtcacaaaat acgtatacgg cgtgacattc ctattcgcca
    11221 ggtcttcatc taaatctgtt aaatcatccg caagctgatt atacatcccg taatgaaaca
    11281 tatttgcttc gatgttatcg acctctgaaa aaggcttaaa cgaacggata atcagtctgg
    11341 aggaagccga ttttaaaata acagatgtat acaggtcttc attggtatac ctgtcattat
    11401 ccaaatgctt ttgcctgtcc agttcctgtg cttcgaaaaa taaatacgca ttttccagga
    11461 aaagctgtct attctcctcc ggctggcagg actttaaata atgaaatgct tccgacagct
    11521 cctgatgcac agcagaaatg aattcccaat gattcttctg ccaatcgtca gcttttactg
    11581 gaagcacctc ccctgtgaat aacgtctgcc ggataaaata agcgaaagtc ttcttctccg
    11641 atgccgttaa catatccgca tccagcacat catcaatcag cggataggtc aatccataat
    11701 aatatcctgt acgaacagcc tgatctaaca gctgcctcct ccgatctttg gacagttctc
    11761 catccaattc ttccaattca tgcataacga caccagcaat aatcttcatt atttttcgtt
    11821 tcgcttcgtt tttatccaaa ccatccggaa gtatcgcgga gacacgttta agtttatccg
    11881 acacccagaa gaatacttct tccagcgatt ctttttgtgc catttggtaa agctctgtca
    11941 gatggaacgg cactcgctgc tccttctgat tcaagtaatt tttgacgcct tctgtgacag
    12001 cagttattct gttctgcatt gcttcagagg taatatccct tcccaggtca cgaaaataaa
    12061 tgtacgaaat acttcgatgc aaataagcat caagctggcc tgatttcgct aaataagaaa
    12121 taaataattt gcttggctgc tgcttccaat gatctatatt tacccatgtt ttctttttat
    12181 cttgttgata ttgatgaagc gtctctatca aggctgatgc aaaattctct tcttgtattt
    12241 gagcctgcag cgtttgaaaa tatgcttttg cctgctcctg ctttgtctga aaataatctt
    12301 tgtccatctg catgttccgt cctcattttt ttgttaagta attaagtata ccacgatgtt
    12361 ccgtacacaa taatgatgca agtaaaactt aacaaaatct ttacatttgt taaaaatatg
    12421 ccgtttacct gacctaaaaa tcccaagtaa aacggcatat ttttttaaga ggttattcaa
    12481 aaagcccgtt aaaaagtact ttttgaatac acactttaac ttatttctat tcatttcgtt
    12541 tatgctctat gaaaaatctt ttttgctatt tccatacaaa ccacagcggc agctgccagt
    12601 cctgttgcaa ttccccattc ctggaaacca aaggaagcag gtatattaaa cacctctctt
    12661 gcaaaaggaa gcagcgttat cccatataaa gcaaacccga ttacaactgc tcctatcaca
    12721 tatttattag agaagaagcc aattttaatc gatgtctgtg tattcgaacg tgcagcaaag
    12781 gtctgcaggg tacgtgacat aatcaacgtc gtaaatgcca tcgccacacc catttcattg
    12841 gaatgggaca aaccaatata gaaagaaata acaacagcag cagcaattaa aatccctctt
    12901 gtcaaaacag tccgcagcgt accgccggca aatatacctt cgttaaccgg acgcggtttc
    12961 cgcttcatca cgcctgcctc tgatttttcc acacctaacg caattgcagg cagcgagtca
    13021 ttgactaaat taataaacaa gagctgcaac gctgtaaatg gattcggcag attgaagata
    13081 acagcataca gaatcgcgag gatggctcca agattcccgg caaataaata accgattgct
    13141 tttttaatat tatcaaatac ggttctcccg acctgaacag cattaacaat agatacaaaa
    13201 ttatcatcgg tcagaatcat tgccgacgaa tccttggata catccgttcc gcttcccatc
    13261 gcaataccga tatccgcctg ttttaaagca ggagcgtcat tcactccgtc acccgtcatg
    13321 gctgttacag cacctctttg ctgccaggct cggacaattc ttattttatt ttcaggtgat
    13381 acgcgggcgt aaacggaaat ctgctcaatt ttgtcctcta attcttcctc tgacatctta
    13441 tcgagatctt ttcctgtgat tgcaatatca tcattttcca ttaagccgat atcccgccca
    13501 attgcctggg cagtagtttt atgatcgccg gtaatcataa tggtccggat cccggctgct
    13561 tttgcttctt caatcgatcc gtacaccgct tcccttggcg ggtccatcat cgcagtcagc
    13621 ccgactaaca caaaatcccg ctcatcctca tacgtaatat ctgtctgatt atcaggcaag
    13681 cgtttataac cataagccaa tacgcgcaga gcctggttgg agaagtcttc attggctcct
    13741 tcaaaccgct ccagcaaatc atcggtcaat ggctgttcct caccatcgag cagcacatga
    13801 cttgcgcgat gaaacatcac atctggaccg cccttggcaa ataacagacg ttccccgtcc
    13861 atatggtgaa cagtggacat cagcttgcgg tcagaatcaa agggcagttc tgcttcccgt
    13921 ggatattttt ttcgtaattc ctgataactg tgcgcatgtt tatccgcaaa ttgaattaaa
    13981 gcaacctccg tcggatcgcc aatctcttgg ttctcctgat taatatacga atcattacaa
    14041 agggctgcaa tatagacaag aaatctctcg gacttatccc attcatccgg attctccggt
    14101 aaacgttcat ttttatgaaa agggataaaa tcatcggtaa cggtcatttt attttgggtc
    14161 agtgtccctg ttttgtctgt acaaataatc cgtgtagatc caagcgtctc tacagctggc
    14221 agcttacgaa taatggcatg ctgctttgcc attttatttg tgccgacaga taagacaata
    14281 gtaacaatcg attgcaaagc ctcagggata gcagcaactg cgatagctac cgagaacatc
    14341 agcgcattta ataatgcagt ggttgtatct gcctcactgt cgccaaacca gatacgagct
    14401 gcctgaacag caaaaatagc aatacataaa atcaaaatgc cgacaccgag cttcttgctg
    14461 aagctgttta acttctgttg taacggcgtt tctttctgct ctgcagtaga aagcatatcg
    14521 gcaattttac cgacctctgt ttggtttcct gtaccggtaa ctacaaaggt tccccgtcca
    14581 taaacaacca aagagctgct gtacaccata tttagtctgt ctcctaaagc agcctcgtcc
    14641 tcaatgatat cggtgttctt ttctaccgcc tccgattcac cggtaagcat tccttcattt
    14701 actcgtaaag agccgctttc cagcatacgt ccatccgcag gtatatagtc tcccgcttct
    14761 aagtagacaa tatcaccggg aacaagctct ttggcagcga ttgttttttc ggttccatcc
    14821 cgcatcacat tcgcatcagg ggcagacata tcccgtaatg cctccagcga gctttccgct
    14881 tttctcgtct gtaccacgct gattaccgag ttaatcagca gaaccaaaaa gataataatg
    14941 gattctaccc attctccaat caatatttgt actgcagccg ccgctaacag gacaataacc
    15001 atcggatctt tgaaggtctc taaaaaaagc ttccacgtcg gtactttttc tttatccgct
    15061 atctcattat atccgtattg atccagtctg gatgctgctt cttcggttga tagcccctgt
    15121 tgggaagcct ctaattcttc caaaacatct gcagcttttt tttgatacat ttccattttc
    15181 aaaatctccc tgcttgttca tttttcaatt atagagtgca tttattgttt attttgcatt
    15241 cttcaacaca ttccatcccc attttcaaaa taatacagca atgaaaggtt tttctttatc
    15301 ttgagctatt gccttttttc attcgattga aaccttatta ctgcagaaaa gaaggaatct
    15361 ttacaaaatc ttcatattta tcttttaaaa atgatttaac ttatcctgct gctatggaag
    15421 ctattatata cttctttttc cttttatgct tgcttggata tactctaatc tgccaaaagc
    15481 caatatagac tttgcaacag tcagtttttg cccatcaaaa aaactgacct tgtctctggt
    15541 cagtttttct gccgatgtta cttctactca ttccccatct caataatccg ctgattccgg
    15601 ctgccacgaa aggataaggt taaatcccgc tcagccaata taaatggtcc gtccaccagc
    15661 acatctacat agttaagaag ctccagctga tgcggatctt tgttctcacg cagctcttcg
    15721 atcgtccatc cagtatacgc ccatatattt ttcccaagcg ccttcaattg tctggcaata
    15781 cttgctgcag cctgtgcctg gtaaaatgga tctccgccgc ttaacgtaat atctgtcaat
    15841 ggattccatg cctcctcgac aatctgctgc acatccatct ccttgccatt ccgtatattc
    15901 caggaccttg gattatggca tcccttgcag taatggggac agccggcaaa gaaaataacc
    15961 gtacgtaagc cctctccatc aacgactgaa tcatgataaa tggataaaat ccgtgctgtt
    16021 tccatcatac atgcctcact ctgtctcgtt cttccgatgc ttttgctgta ttccatttat
    16081 ccattgttcc taccagatag ccggtaatac ggcgaatccg gtcaaattgt ctttcatccg
    16141 cgatttgaca gcttggacac tcgttatcga tcgttccggt atagccgcag ctttgacagc
    16201 ggtcgaccgg atggtttaca ctgccatagc caatcccgct ttcagccatg gttttcacaa
    16261 gggtgtcgat agcttttata tttttgctga catctccgtc tacttcaata taagtgatat
    16321 gacccgcatt ggtatatggg tgatagacag cttcccgttt tattttctcc acagctcgaa
    16381 tcggatagta cacaggaata tgaaacgaat ttgtataata atctctgtct gtaattccgt
    16441 ctctttttcc aaaatccttg caatcctgct tcacaaatcg gccactcagc ccttctgccg
    16501 gtgttgcaat cagcgaataa ttcaaatcat agtcttctgc agcctcgtca catttcttcc
    16561 gtaaaaaggc aacaatctct tctcccagct cctgcgcctg ttctgattct ccatgatgct
    16621 tgcctgtcaa tgcttttaaa cactccgcca ggccaataaa accaacactt aaactcccct
    16681 gcttcaatac ctcttccaat gtatcctctg gatttaattc gtcactgcct gtccagacgc
    16741 cttctccatg caaaaaagtg aagttattca caagtttatt tgcctgaaat acataccgtt
    16801 catacaattg acgaatgacc atttccgtat agacctctaa tttcgccata aatgcctcga
    16861 cggttgatga ggtcagcgct attttcacta aattaatact ggtaaaggag atattgccgc
    16921 gcccgagact attttcttct ccatgacgat tagccataat acgcgtacgg cagcccatat
    16981 aagctacttc cgaatccgga tttccttctt cataataaac gaagttgaat ggcgcatcga
    17041 taaagctgaa gtttggaaaa agcctttttg ctgttgtttc caaagccagc tgatacaaat
    17101 catagtttgg atccgcttta taaaaattaa cgtctttttt cattttaaaa atttgaatcg
    17161 gaaaaattgg tgtctcccct tttcctaaac cagcctgcgt agctaaaaga agatttttta
    17221 taatcagtct tccttctctg cttgtatctg ttccataatt gattgacgta aatggaacct
    17281 gaccgccgct tctggaatgg aggctgttca aattatgaat aaacgcttca caggcctggt
    17341 agcattcccg gtccgtttcc tcccaagcta acttctccaa ttcctgtgga gcaaggggaa
    17401 gctgatattg ctgtaatcta tgcagccgct tttgatagga cttcttcaca aacggcgcta
    17461 aatcataatc aaacgcctgg aagctctgtc caccatgctg ctgattttgg ttgctttgaa
    17521 aaacgattgc cgctaatgcc atcgcactca gaatgctttg tggggagcgc atatgtccat
    17581 gccctgtgtt aaaccctttt tccaacaatt ttcctaaagg aatttggcag caggtcgctg
    17641 tccccgtggg catataatct aaatcatgaa tatgaatata attatcctcc attgcctgct
    17701 tcgcttctcg gctcaacatc ttttctttcg cataaagttt tgcactttca ctagcaaact
    17761 tgttcatctg cccgcatggt gatttcccgt ccacattagc attctcctgt attaaatcct
    17821 gattggtgct ggatacaatc tcttgaaaat cctgcattaa atctgcttct gtactttcag
    17881 gagcagtcat tacttccgcc atcttctatt cctccttctt cgtctccgca aatacgtttc
    17941 aacgcactca aaaactatat atagtatgtt taaaaacaaa tcagaactat atatagttct
    18001 atggtaaagg agggtagaaa gaagttcaaa tggatatttt atgactttta ccactttttc
    18061 ttctcattct ctatcccgat tatgaatttt ttaagtcatt tttgaaccgt ttcaagcatt
    18121 tcttccagaa gctgctgccg gttaccctgc cgcaggaatt cctcttctgt aattttctgc
    18181 agaatctgct ttttttgctc gggtgttaaa ggcagggtga ggattctttt tcttacctga
    18241 tcaagaaaga taagatcagc ttctatttct gctggcagct gatccgccag cttatttttt
    18301 aactgttttg cataaattgg actcgcaccg ccggtggaca acgcaaggct tagcgctccc
    18361 ctttgtagaa cagcaggaaa ggatacatca cttttcatgg catcatttgc aacataagcc
    18421 agctgatgcg gctgtttgtt ttcataaagg gacacattca gttctttctt atctgtcatc
    18481 aataaaagaa atagacaatt aaaatgttct ccctcgtgaa cctctctttc ctgccacttg
    18541 atttttcctt tttgataaag ctgctgcaaa aatggatgaa gcttcggcgc aatgatagtg
    18601 atagcagctc cagcatctaa aaaatgacgt atttttctcg ctgcaacagc tcctccgccg
    18661 actgcaatga cggtctgatt cttcacctct aaaaaaagcg gcatgcgcat cagctgactc
    18721 ttcctttcag caatgtattg actcgatcga ctaggactgt aacgagctga tcatcaaatc
    18781 ctaagtacgg acaaagaata aattgtttat ctgtccgttc agacagttca tctaatttct
    18841 ggcgcagctc cctcattaat acaccggaga atagcaaata ggggacaaca tacaccaccg
    18901 aattaggact tttaagcaat tgctccagcc cctcatccaa tgtaggacgg gttgctgcca
    18961 gataacatgt atttacttca tttaccggag ctgcctccca taataagcgt gcaattttgg
    19021 caaggtcgct attggcatcc ggatcactgc tgccccgccc gacaagtaat acagaagcag
    19081 gttcgcgttc ttcatacaca ggcggtttac cggtttgctt cagtcctccc atttccaggc
    19141 gtttgtttag taaatcaatg attgccggat ctactcccat cacctttcca taggtaaagg
    19201 ttacgtctgg atagcgtttt tctgcctgcc ctatttcttt tgggatatcc tgctttgcat
    19261 gcccagctga aagcaataga accggcacaa ctgcaatctg tgctgctccc tgccggacac
    19321 agctgtcgat gcctgtttgg atggatggtg aagcaagttc aataaagcag gtttcctgta
    19381 tctgaatctg cggatttttt ttcttcgctc tgtctacaaa agccagaagc tcctcattcc
    19441 cccgttgtac ccggcttcca tgcccgatat ataaaatagc ctgcatcctg tattcccctt
    19501 cctctcattc tctttcaagt ctagcaaatt tctgcttaat gaacgatatt aattgtaaaa
    19561 cttaaaaatt ttaagaaaag gccgctgcag ccagcggagc ttctttttgc aaaagaactt
    19621 cctgcagtgc ttcttcggtc ggttcagata atacctccga aacagcatac cctgctttga
    19681 cagcagcctg ctttgatttt tctccaaaac aaatcacgcg tgcataggta ctaatgtttt
    19741 ctggcgtttc tccacaagct tgaaaccctt ccgtcactgt tttcacagaa cgcgcattag
    19801 gaaaaatgat cgtttcgata cgctcctcct cctgaagacg gctgaacgtt tgctccgtct
    19861 gctgcacacg aataaagcta tgcgtagcaa tgaaatcagg ctcaaatacg aatggataac
    19921 gctgctcttt tcgatggaag ccttccgggt ctcctaatat taccagcggg gaagcgtcgg
    19981 ggctccgttc tgctatttct tttccaaaac aatgatagga ttctatggcc tttactgttt
    20041 ttttagaaat tccatagaat tcaccttgaa tccttcgaat atcgatttta tttgcctgca
    20101 gagactcgaa gaaaatgtcc acacttgccg gtgataaaaa aagaagatcc cgatttgccg
    20161 gaatatcggc ttttttcaac agatgctttt tcttttgcat ccgcggaaat tcaaaaacct
    20221 ctgctccctg ctcccgtaaa tgccgggcca tttcactcgg ttcttcacca gcccgcccga
    20281 gaagcaaatg ctgactaaac agcggcttgc tttcaaacca gctttctcct tttttcagtg
    20341 tgcttacatc tcctactaag atgatagctg gatttttaaa ctgtgccgct tttgctttct
    20401 gtacaatagt tgcaagattt ccctgcagcg ttctctgttt tccggtcgtg ccccattgaa
    20461 ttaataaaac tggcgtatcc gaagaccgcc catatgagat gagctgttct gaaatgtaag
    20521 gcagattgcc caatcccata tagaacgcaa tggtgtcgat cccgttcgct aaagcatgcc
    20581 agtcaatcag cggttcacct gaaggcgact tatcatgacc tgtaataaca gcaaaggata
    20641 agctatgctc acgatgtgta actggcacgc ccgcataagc agcagctccg attcccgccg
    20701 ttatccctgg aacaatctcg taaggcacac cggcctgttc cagtgcagct gtctcctcgc
    20761 ctacccggcc aaatacgctt gggtcgccgc ctttcaaacg cacaacattc tttccttcta
    20821 agccatgctt tactaataac tcattaattg cttcctgacg cagtatatgt tttttaggca
    20881 ttttaccaca atatataaat tctgcttgcg gtgaagcata ttccagtaag agagggttaa
    20941 ccagcctatc gtataaaaca acttctgcct gctgcaaata cgtataacct tttaaagtca
    21001 ttaacccaat atctccagga ccagctccga ctaagtacac acatcctggt tttgtcatca
    21061 ttcgtcaccc tcatttcatc catttctaag ctttttcgta tcaaatcggc ttttcaagtc
    21121 aatagccctg cttttcttaa agttaaagaa gagcagggcg ataaccgcag ccctgtctct
    21181 tctttaaagt tttcaacctt aaaacgttta atcatgaaag tttactccaa aagtaacagc
    21241 cttcacatac tcttttcgaa ctacaaaatc accgaagtgt tcgccatcca gacgttcctt
    21301 tgcataatgc cgaataatcg gctgcagcgt ttctaaaatt tctttttctc caatgctttc
    21361 ccggtaaatc ttgcttaaac gctgtccggc aaatcctgct cctagataca tattatattt
    21421 tcctggggct ttcccaataa agccaatttc tcctaatgct gctcgtgagc atccgttcgg
    21481 gcagccagac atccgaatga caatttcttc atcacgtagc ccttcttctt ccagcagtgt
    21541 ttccagtttc tcaatcaagg aaggcagata acgctctgat tcagccatcg ccaagccgca
    21601 ggtcggaaaa gcaacacaag ccatggaatt acggcgtaat gcagattgct cctttccatc
    21661 tgacagtcca tactgctcga taagtgcgat gatttccttt ttcttctgac tggtgacatt
    21721 tccgattacc aggttttggt ttgccgttaa acggaaatct ccggtatgaa ttttggcaat
    21781 ttcccgcagt cccgtcatca gctgatagtc ttctttatcc tgaatacgtc cattttgaat
    21841 aaaaagcgta aaattccagc gattcttcgt ccctttcacc cagccatagc ggtcgccgtt
    21901 atggtcaaaa tgaaagtcgt gtgcttcgtc cagctgccag cctaaacgtt ccgtcagctc
    21961 atttttaagc caatcaatcc ctctggcatc aatcgtatat ttaaaacgtg cgtattttcg
    22021 ttcggaacgg tttccataat cccgctggat ggtaatgatt ttttcagcaa catccgtgat
    22081 ttggtccggc ttacagaaac caatcactct tcccagctgc ggatacgtat tggtatcgcc
    22141 atgtgtcatg cccataccgc cgccaaccga tacattgaat ccttgaagct gtccatcctc
    22201 cagaacagca ataaaaccta aatcctgcga aaagatatct acatcattgg atggcggaac
    22261 tgcaattcca atcttaaact ttcttggcag ataaaccgtt ccatacattg gct
//
