LOCUS       NZ_CP101913            10387 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Oceanobacillus oncorhynchi strain QA-1986 526 chromosome, complete
            genome.
ACCESSION   NZ_CP101913
VERSION     NZ_CP101913.1
KEYWORDS    RefSeq.
SOURCE      Oceanobacillus oncorhynchi
  ORGANISM  Oceanobacillus oncorhynchi
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Oceanobacillus.
REFERENCE   1  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4669308)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C., Franklin,A.B. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 237.47x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024508135.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 08:40:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,482
            CDSs (total)                      :: 4,384
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 39 of 69
            Pseudo Genes (internal stop)      :: 13 of 69
            Pseudo Genes (multiple problems)  :: 13 of 69
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:15:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2018519
            Orig. end                         :: 2028906
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101913.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5387](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5387)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(152..322)
                     /locus_tag="NP440_RS10075"
                     /old_locus_tag="NP440_10075"
     CDS             complement(152..322)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651877.1"
                     /locus_tag="NP440_RS10075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10075"
                     /product="YfhD family protein"
                     /protein_id="WP_084612954.1"
                     /transl_table=11
                     /translation="MGRDEHKKRKNNFLSQTPKNQISDGIDVEFSDEFADAEDRKAQAR
                     SRAADKRAHEK"
     misc_feature    434..451
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            670..903
                     /locus_tag="NP440_RS10080"
                     /old_locus_tag="NP440_10080"
     CDS             670..903
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010651861.1"
                     /locus_tag="NP440_RS10080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10080"
                     /product="hypothetical protein"
                     /protein_id="WP_042533609.1"
                     /transl_table=11
                     /translation="MSNNEKRSSKSGKPNRNEERRTMLDEHEEQMFVDDVPLEDLKIEA
                     KDEKRKTKSKDDSQSEKKHKEKQNKDNDSKKP"
     misc_feature    1048..1064
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            1275..2798
                     /locus_tag="NP440_RS10085"
                     /old_locus_tag="NP440_10085"
     CDS             1275..2798
                     /GO_function="GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016913214.1"
                     /locus_tag="NP440_RS10085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10085"
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="WP_256706148.1"
                     /transl_table=11
                     /translation="MEHMGWLSLVPPILAVILAILTKNVIISLFLGAFVGVLIIVGGNP
                     LTATTETIGNFLFPQLTDSYNAAVLVLLFFIGGFVALIEKSGGGAALAAKTVKSINSKV
                     KAQLSAWLGGLIIFFSDLGTPLIVGPIFEKIFDKARISREKLAYIIDSTSSPVAVLIPF
                     IGWGVYIMGLIRSEFDALGITTSEFDTFVQVIPFQFYAISAVLMIPLMAVFKLDFGPMA
                     KAERRVRETGELYWKDSKPLRRPDKEADSNQEGKAILIWLPLSVLFVTLFGLLSTKGFP
                     FEPVDGNDFRVSLTTAYLFAAITIFVLLISFRLKKFAEIFDIYTSGMQKMVYVAATLVL
                     AWSLGTVISEMGTANYIVELMQGRVPAFIIPALLFIIGAIVSLASGSSWGTFAIMLPIA
                     IPMAVALDAQVLVCIGAVLSGGIFGDHSSPISDTSILSSTGAGADHIDHVKTQFPYALT
                     NAIIAIIGFVIAGVTGSPWTVVLTVVLLVFAAFVLSKLTKNKYESKMNI"
     gene            2866..3300
                     /locus_tag="NP440_RS10090"
                     /old_locus_tag="NP440_10090"
     CDS             2866..3300
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640391.1"
                     /locus_tag="NP440_RS10090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10090"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_256706149.1"
                     /transl_table=11
                     /translation="MQWKKPASEDYAALIELWEKSVLATHDFLASEDREALKKEIPAYF
                     PQLDMKMWFEDTTFVGFSGVNASQLEMLFLDPEQIGKGLGSRILNDLIEKDGIKTADVN
                     KDNTSALAFYLKNNFQVTGESEQDDQGRDYPILHLCLKAE"
     gene            complement(3434..4780)
                     /locus_tag="NP440_RS10095"
                     /old_locus_tag="NP440_10095"
     CDS             complement(3434..4780)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017811636.1"
                     /locus_tag="NP440_RS10095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10095"
                     /product="MFS transporter"
                     /protein_id="WP_256706151.1"
                     /transl_table=11
                     /translation="MRTVQTTQVLGNSKFNKFHLLIFLWCFFAIAFDGFDVAIYGIGLP
                     LMMEDFNISRIEAGAISSYTLISTMVGTFLLGSISDFIGRKKAIAICLILFSLFTFLAG
                     FAQNPTLFMIMRVIAALGLGGIMPILVAIMGEYSPVKKRPLTIATMYCGYSIGAIIASL
                     IGMYLMEMMGWRLLYWISIIPLLALPWFLKQFPESVSYYLTKKKGDKIAEILNKVDPGG
                     NYQASDNYEYESFKESTKEIPIKKVFSNKRTSSTIAFWIAVTCSMLVISGLTTWLPQIM
                     VDAGHGLTSSLSFNLMLAVGQISGSIFGGMLVNRLGHRSVLILMFFIGSLCFVLLSITT
                     SSLLLYLIITLTGACTVGTQNLVNPYISEFYPREIRTTGLSIAVGVGRIGGIMAPVAIA
                     LLLSTNLDPQHAFMAFAVPSLIGAVCFITVREKHASFDQAVPVKKHQIA"
     gene            complement(5001..5387)
                     /locus_tag="NP440_RS10100"
                     /old_locus_tag="NP440_10100"
     CDS             complement(5001..5387)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064922.1"
                     /locus_tag="NP440_RS10100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10100"
                     /product="ectoine synthase"
                     /protein_id="WP_042533613.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.5e-51, bitscore:
                     169.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKSLEDLIGTEDETKSENWESRRFILNKDGVGFSMNDTIIKAG
                     TDNFFWYKNHIEAVYCIEGEGEVEKVSTGEVHQLKPGTMYLLDENDKHRLRARTQMRMV
                     CVFNPPLVGTETHNKEGYYPLLTE"
     gene            complement(5419..6666)
                     /gene="ectB"
                     /locus_tag="NP440_RS10105"
                     /old_locus_tag="NP440_10105"
     CDS             complement(5419..6666)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-356). E-value: 2e-79.
                     Score: 259.0. Matches aSDomain:
                     nrpspksdomains_NP440_RS10105_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379253.1"
                     /locus_tag="NP440_RS10105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10105"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_193064157.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.3e-80, bitscore:
                     269.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTFENLESEVRSYSRGWPTVFEKSKGYKLWDTDGKEYIDFFAGA
                     GTLNYGHNDDAMQEKLIEYIKNDGVIHSLDMATIPRKDFLERFTDVILKPRNLDYKIMF
                     PGPTGTNTVESALKIARKITGRETVISFTNAFHGMTIGSLSVTGNSFKRHGAGVPLHHA
                     VSMPFDNYVEDNDSIAYLDRFLEDSGSGVALPAAIILETVQGEGGINAARMEWLKKVEA
                     LCRRWDILLIVDDVQAGNGRTGTFFSFEPAGIKPDVVCLSKSIGGIGLPMAITLIKSEY
                     DQWGPGEHNGTFRGNNMAFLAATEALKNWENDDFANAVQEKGAFLKSEINRLIDHYPEL
                     NGEARGRGLMQGIAVEKEGLAGEICAEAFERGLIVETSGPDDEVVKFLPPLIIDQEGLV
                     KGLDILEDSIKHVLKK"
     aSDomain        complement(5599..6603)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NP440_RS10105_Aminotran_3.1"
                     /evalue="2.00E-79"
                     /label="NP440_RS10105_Aminotran_3.1"
                     /locus_tag="NP440_RS10105"
                     /protein_end="356"
                     /protein_start="21"
                     /score="259.0"
                     /tool="antismash"
                     /translation="FEKSKGYKLWDTDGKEYIDFFAGAGTLNYGHNDDAMQEKLIEYIK
                     NDGVIHSLDMATIPRKDFLERFTDVILKPRNLDYKIMFPGPTGTNTVESALKIARKITG
                     RETVISFTNAFHGMTIGSLSVTGNSFKRHGAGVPLHHAVSMPFDNYVEDNDSIAYLDRF
                     LEDSGSGVALPAAIILETVQGEGGINAARMEWLKKVEALCRRWDILLIVDDVQAGNGRT
                     GTFFSFEPAGIKPDVVCLSKSIGGIGLPMAITLIKSEYDQWGPGEHNGTFRGNNMAFLA
                     ATEALKNWENDDFANAVQEKGAFLKSEINRLIDHYPELNGEARGRGLMQGIAVE"
     gene            complement(6714..7232)
                     /gene="ectA"
                     /locus_tag="NP440_RS10110"
                     /old_locus_tag="NP440_10110"
     CDS             complement(6714..7232)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379254.1"
                     /locus_tag="NP440_RS10110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10110"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_319022553.1"
                     /transl_table=11
                     /translation="MTIEKENDFYFREPAKDDGAAVWELIKATKVLDLNSSYSYLMWCD
                     IFSGTSIIAEKNGKTLGFISGYKNPNNPNKLFIWQVAVDASARGNGLATKMLRQLLKRE
                     ACKKIDYVEATVSPSNQPSQSLFKGLAKKLDTECHISDCYVTDDFPDEEEEHEDELLFT
                     IGPIATENK"
     misc_feature    7204..7218
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.67"
                     /tool="antismash"
     misc_feature    complement(7206..7217)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.88"
                     /tool="antismash"
     gene            complement(7607..8335)
                     /locus_tag="NP440_RS10115"
                     /old_locus_tag="NP440_10115"
     CDS             complement(7607..8335)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066496.1"
                     /locus_tag="NP440_RS10115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10115"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_042533616.1"
                     /transl_table=11
                     /translation="MKKKIFYAISYLLLTSCAFLFIQEQIYAKDRFDFEKDGDIFWDAK
                     TEEKVIALTFDDGPHSKYTPAILNILAEYEAAATFFVLGAHAEKFPDIIHRQVMEGHEL
                     GNHTYDHTMKLSRLSQEMKETSDILYAITGNRPKLFRPVGGSYNDAIVKKARENQLHVA
                     LWSWDQDTKDWDGVDYKTIADNVISNISPGDVVLFHDAGGKREQTVKALPLILDYLAEN
                     QYKTITVSEMMEMDEKLKEN"
     gene            complement(8713..8895)
                     /locus_tag="NP440_RS10120"
                     /old_locus_tag="NP440_10120"
     CDS             complement(8713..8895)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095651.1"
                     /locus_tag="NP440_RS10120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10120"
                     /product="hypothetical protein"
                     /protein_id="WP_042533617.1"
                     /transl_table=11
                     /translation="MDNNKRNFNRNERKNRNNENRNNQEINNVEFANENEFIDENAFED
                     NNNHNNERRNNRNRK"
     misc_feature    complement(8857..8868)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            complement(9043..9240)
                     /locus_tag="NP440_RS10125"
                     /old_locus_tag="NP440_10125"
     CDS             complement(9043..9240)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /locus_tag="NP440_RS10125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10125"
                     /product="amidase family protein"
                     /protein_id="WP_052485043.1"
                     /transl_table=11
                     /translation="MKGFEKAFKQVDILLGPNIPITPHIYSEQWVEQNLEVIRRCLPFV
                     PVNLTGTPSLAVPMGLDGKK"
     gene            complement(9237..9785)
                     /locus_tag="NP440_RS10130"
                     /old_locus_tag="NP440_10130"
     CDS             complement(9237..9785)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /locus_tag="NP440_RS10130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NP440_10130"
                     /product="amidase family protein"
                     /protein_id="WP_338112861.1"
                     /transl_table=11
                     /translation="MRLPASICGIYGLKTTYGLVSTHGIFPTAMTLDTASPMARSVSDL
                     VCMLYYMAGFDANNPGSLDVSVPNYIYDLNNGIQGIKIGTPAYFLQDLAPDIEILFKHA
                     METLLYLGAEIVDVEIPELALSTFSGYTIAAGEAAALHHQWLQTHSNEYSADIRSFFLA
                     GALTETPQYIRRSRPGDNW"
     misc_feature    complement(9810..9823)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: medium, score: 18.8"
                     /tool="antismash"
     misc_feature    complement(10341..10354)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.52"
                     /tool="antismash"
ORIGIN
        1 ttttcctaaa tacaacggaa atatacagga agaaagtaaa taaaaaatcg taatatttgt
       61 acgcggactt tgagaggtaa gaggtagaag tactaaagga taacgtaacg gagcgattcg
      121 gttattgaac cgagtcgctc cgttgtacca attatttttc atgtgcccgt ttatcagcag
      181 cgcggcttct tgcttgagct tttctgtctt ctgcatcagc aaactcatct gaaaattcaa
      241 catctatgcc gtcagatatt tgattttttg gagtttgtga caaaaaatta ttttttcttt
      301 tcttatgctc atctctgccc attgggaaca ctccttttag gttgtatata attggttagt
      361 cgaacattcg gccagacaga ttgaagattg caatacatcg agccgctctg tgaaggcagc
      421 tgctgcaccg ccttatcccc tagataacat aagggaaaac tgtactgaca tatctgtatt
      481 tttaacaggt ttacgatact tattcatttt ttccgctggt aaaattagaa catactgtac
      541 cgcttaaact tgtggcagta atctgtgacg gttattaaaa tttgttcaag cagaaatttt
      601 cgcataataa tgtttagtaa tctgtttaga aaggaaaagt attaaaaaga cacagagagg
      661 tggatgaaaa tgtccaacaa tgaaaagcgt tcttcaaaaa gcggaaaacc gaatcggaat
      721 gaagagcgaa gaaccatgct ggatgaacat gaagagcaga tgtttgtcga tgacgttcca
      781 ttggaagatt taaaaataga agctaaggat gagaaacgaa agacgaaatc gaaggatgat
      841 tcgcaaagtg aaaagaagca taaggagaaa caaaataaag acaacgacag taaaaaaccc
      901 tgagcattct tgctgagggt ttttttgata tggagagaat ctcttgaaat tcattagaag
      961 ctgccatcgc aatgaattaa gttctctata aataaataga cgggtttaac tgtgtagcca
     1021 gttattcagt ataattattc gttaaaatgc ataaatatta ggtattttgt ttcatttatt
     1081 tttatctttt ccgtgataga ataaggaaca tatattaatt agcaataatt tttattttca
     1141 atttatcatg gagaaaagca aaactccact gaatgaagtt tcgttatctt atgagatgaa
     1201 ataactgata aggatgtatg tacatatcat tcatacttca tttagcaaag catcaagaaa
     1261 gaaggagagg acagatggag catatgggat ggctatcgct agttccgcca attttggcag
     1321 tgattttagc gattctaacg aaaaacgtta ttatttcatt atttttaggc gcctttgtcg
     1381 gcgtacttat tattgtagga ggaaatccat taactgcgac aacagaaacg atagggaatt
     1441 ttctttttcc gcaactgacg gacagctaca atgcggcagt gcttgtcctg ctatttttta
     1501 tcggaggatt tgtagcttta attgaaaaat cagggggagg cgctgcactt gcagcaaaaa
     1561 ccgtcaaatc gattaattcc aaagtgaaag ctcaattatc tgcctggctg ggcggtttga
     1621 ttattttctt ctctgatttg ggaacacctt tgattgtagg ccctattttt gaaaaaatat
     1681 ttgataaagc aagaatttcc agagagaagc tggcgtatat cattgattcc acgtcatcac
     1741 cagttgcagt actgattcct ttcattggct ggggcgtcta tattatgggg ctgataagaa
     1801 gtgaatttga tgcgctggga ataaccacat cggaattcga tacctttgtt caagtcattc
     1861 catttcagtt ctacgctatt tcagcggtgt taatgattcc attaatggcc gtatttaaat
     1921 tagattttgg tccgatggca aaggcagaac ggagagtgag ggaaactgga gaactgtact
     1981 ggaaggattc caagccgctg cggagaccgg ataaggaagc agatagtaat caagaaggca
     2041 aggctatttt aatttggctc cctttatcgg tcttattcgt tacattgttt ggcttgcttt
     2101 ccacaaaagg atttccattt gagccggtgg atggaaatga ctttcgtgtc tcgttgacca
     2161 cagcttatct atttgcggct attaccattt ttgttttatt aatcagcttc cggctgaaaa
     2221 agtttgcgga gatattcgat atttatacct ccgggatgca aaagatggtg tatgttgctg
     2281 cgacattagt gcttgcatgg tcgcttggaa cggttatcag tgagatgggg acagcgaatt
     2341 atattgtaga attgatgcag ggaagagtgc cggcgtttat cataccagca ttgctcttta
     2401 ttatcggggc aatagtttca ctggcttccg gttcttcctg gggaaccttc gctattatgc
     2461 ttccgattgc tatcccgatg gcagttgcat tagatgccca agtgcttgtc tgtatcggcg
     2521 ctgtattgtc aggcggtatt ttcggagatc atagctcccc gatatctgat acaagtattc
     2581 tgtcatcaac aggagctggt gcagatcata tcgatcatgt gaaaacacag ttcccttatg
     2641 cattgacgaa tgcaatcatt gcgataatcg gttttgtgat tgcaggtgtg accggcagcc
     2701 cgtggaccgt agtactgaca gttgtattgc ttgttttcgc agcttttgta ttatcaaagt
     2761 tgacaaaaaa taaatatgaa tcgaaaatga atatataatt gaaacatgcc ttgctggtaa
     2821 agtgaggcat gttttatttc aattttttaa acggaggaag taataatgca atggaaaaag
     2881 cctgccagtg aggattacgc tgccttaatc gaattatggg agaaatctgt tttggcaaca
     2941 catgattttt tagcgtctga ggatagagag gcacttaaga aggaaattcc tgcttatttt
     3001 ccgcaattag atatgaaaat gtggtttgaa gatactacat tcgttgggtt ttccggtgtg
     3061 aatgcgtctc agttagaaat gctgttcttg gacccggagc aaatagggaa ggggcttggc
     3121 agcaggattt taaatgattt aattgaaaag gacggtatta agacagcaga tgtcaataaa
     3181 gataatacat cggctcttgc tttttacctg aaaaacaact tccaagttac tggagagtca
     3241 gaacaggatg accaggggag ggattatccg attctgcatt tgtgtttgaa agcggagtaa
     3301 tatatgggag tgaaattgca aggtgaggag agcgagtgtt ctttttaaca atagcatagg
     3361 aaaaaggacc attaaataaa gccgcttgct tcttcgtgaa gcagcggctt tatcttatag
     3421 taaattcttt tcgttaggca atttgatgct ttttcactgg tactgcctgg tcaaagcttg
     3481 catgtttttc tctgacggta ataaagcaga cagctccaat caagcttggt acggcaaatg
     3541 ccataaatgc atgctgcggg tctaaattgg ttgataacag aagggcgatg gctaccggcg
     3601 ccataatacc gccaatccgt ccaacgccga cagcaatact taacccggtc gtacgaatct
     3661 cccgcggata aaattccgaa atatacgggt tcactaaatt ttgagtacct actgtacatg
     3721 cgcctgttaa ggtaatgatt aaatacagta agagcgagct tgtagtgatg cttaataaaa
     3781 caaagcagag tgaaccgata aaaaacatca ggattaagac agaacgatga ccaagacgat
     3841 tcactaacat cccgccgaat atggaaccgg aaatttgtcc gacagcaagc atcaggttaa
     3901 aggataagct ggatgttaga ccgtgaccgg cgtccaccat aatttgcggc agccaggttg
     3961 tcagtccgga aataacgagc atggagcagg taactgcaat ccaaaatgca atggtactgc
     4021 tggttcgctt attagagaag acctttttaa tcggaatttc cttcgtgctt tctttaaatg
     4081 attcatattc atagttatca gacgcttgat agttaccgcc aggatcaact ttattgagaa
     4141 tctctgcaat tttatcgcct tttttcttgg tcagataata agaaacggat tccggaaatt
     4201 gctttaaaaa ccatggcaga gcaagaagcg gaataatact aatccaatat aaaagcctcc
     4261 agcccatcat ttccatcaaa tacataccaa tcaaggaggc aataatcgcg ccgatggaat
     4321 aaccgcaata catcgtagca atggttagcg gccgcttctt caccggagaa tattcaccca
     4381 taatagctac gaggattggc atgattcctc ccaatcccag cgccgcaatc actctcatta
     4441 tcataaaaag ggtggggttc tgtgcaaatc cggctaaaaa ggtaaagagg ctgaataaga
     4501 ttaagcaaat cgcaatcgct tttttacgcc cgataaaatc tgaaatagag cccagcagaa
     4561 aggtgccgac catagtactg atcagcgtat agctgctgat tgcaccagcc tcaattctgc
     4621 tgatattaaa atcctccatc atcaatggca gcccaatccc atagatggcc acatcaaagc
     4681 cgtcaaaggc aatggcgaaa aagcaccaga gaaaaataag taaatgaaat ttattaaatt
     4741 tactattccc taaaacctgt gttgtctgaa ccgtccgcat gtttaaaacg tcctttcctt
     4801 taattgatcg aagtgtaata agtaaataaa atattaccat gaaacaatgg ttataaggta
     4861 attgtaaaaa ggtatcgttc gctataggta aaagctatat caataaagga gaggatagaa
     4921 gcagttgttt gtaataaata aggtacgtaa aaaaatcctc tgatagaagc tattcttatc
     4981 agaggatttt agaaagtaat ttattctgtt aaaagcggat aatatccttc tttattatgc
     5041 gtttctgttc caactaacgg cgggttgaag acacatacca tacgcatttg ggtgcgtgcc
     5101 cgcagacggt gcttatcatt ttcatctagc agatacatcg tgccaggctt aagctggtgt
     5161 acctctccgg tggagacctt ttcgacttct ccttcacctt caatgcaata aaccgcttca
     5221 atatggttct tataccagaa gaaattatct gtccctgctt tgataatggt atcattcatg
     5281 ctgaagccga caccatcttt atttaaaata aagcggcggc tctcccagtt ttccgatttt
     5341 gtttcatctt ctgttccaat taaatcttcc agtgatttta cgatcattta taaattctcc
     5401 tctccgtaaa ccatatctct atttttttaa aacatgcttt atactgtctt ccagaatgtc
     5461 taaacctttt accaaacctt cctgatcaat aatcaaaggc ggcaggaatt tcaccacttc
     5521 atcatctgga ccagaggttt ctacaataag tccgcgttca aatgcctccg cacaaatttc
     5581 acctgctaaa ccttccttct ccacagcaat gccctgcatt aaacctctgc ctctagcttc
     5641 tccgtttaac tccggatagt ggtcaatcaa tctgttgatt tctgatttaa gaaatgcgcc
     5701 tttttcttgt acagcattgg caaaatcatc attttcccaa tttttcagtg cttcggttgc
     5761 agcaagaaag gccatattat tgccgcggaa ggttccatta tgctcgcccg gtccccattg
     5821 atcatattct gacttgatta acgtaatcgc catcggcaga ccaattcctc caatggattt
     5881 agacaggcag actacatctg gtttaattcc cgccggctca aagctgaaga aggtgcccgt
     5941 ccggccattt cctgcctgga catcatccac aatcaggaga atgtcccagc gtctgcatag
     6001 tgcttctact tttttcagcc attccatgcg tgcagcatta atcccgcctt ctccttgaac
     6061 ggtttccaga atgatggcag cagggagggc aacaccgctt ccgctgtctt ctaaaaagcg
     6121 atccagataa gcaatcgaat cattgtcctc cacataatta tcaaatggca tcgaaacggc
     6181 atgatgcaat ggaactccgg caccatggcg cttgaaggag tttcctgtca cagaaaggga
     6241 gccaatcgtc atcccgtgaa atgcattggt aaagctgata actgtttctc gtccagttat
     6301 ttttctggcg attttcagcg cgctttctac ggtattcgta ccggtagggc cagggaacat
     6361 aattttataa tccagatttc ggggcttgag aatgacatca gtaaagcgtt ccagaaaatc
     6421 ttttctggga atcgtagcca tgtccaggct gtgaatcacg ccgtcatttt taatatattc
     6481 aattaatttc tcctgcatcg catcgtcatt atgcccgtaa ttcaatgtac ctgcgcctgc
     6541 aaagaaatca atatattctt ttccatcggt atcccacagc ttatagcctt ttgatttttc
     6601 aaaaacagtc ggccatcctc tgctgtagct tcttacttca gattctaaat tttcaaacgt
     6661 tttcattttc gcatcattcc atgttgctgt tgtcaatgaa atcattcctt taattattta
     6721 ttttctgtag caatcggtcc aattgtaaac agtagctcat cttcatgttc ttcctcttca
     6781 tccggaaaat catccgttac atagcagtca ctgatatgac actccgtatc aagcttttta
     6841 gcgagacctt taaataaaga ctgagaaggt tgattggatg gagaaacggt tgcttctaca
     6901 taatcaatct ttttacaagc ttcgcgcttt aataattggc gcagcatttt cgttgctaat
     6961 ccgttacctc gagcggaagc atctacagca acttgccaga taaatagttt attcggatta
     7021 ttcggatttt tatacccgga aataaaaccg agcgttttcc cgtttttttc tgcaatgatg
     7081 gaggttcctg aaaagatatc gcaccacatt aagtaactgt aggaagaatt gagatcaagc
     7141 accttggtag ctttaatcag ttcccaaaca gctgccccgt catcttttgc aggttctcga
     7201 aaataaaaat cgttttcttt ttcaatagtc attgtcatat tatttgttgt gtttgtatta
     7261 atgctctcac ctcctgagtg gattttttac ttaccatatt agattaacat aaggaaaaat
     7321 aacgtcaaaa cgcatataac ggagaataac gcgattattc gtgataagaa ggggtgaggg
     7381 taaaatgatt gccattggct ataaatagct ttgaatataa cagaaataat ccgttttata
     7441 aacttttggg gaaaataaaa taatgaatta aatagataaa ccatattgtg gggatagaaa
     7501 aggataactg taagctaaaa atttattatt gaagaacaaa acagctgtcc gctcattttt
     7561 aggggagtaa atgattaagg gacagcaata ataaagcttg aattgattaa ttttctttta
     7621 atttttcatc catttccatc atttcagata ctgtaatggt tttatattgg ttttcagcta
     7681 aataatcaag aataagcggg agagctttaa ctgtttgctc tctttttcct cctgcatcat
     7741 gaaacagtac gacatccccc ggggagatat tgctgatgac gttatctgca atggttttat
     7801 aatctactcc gtcccagtct ttcgtatctt gatcccaaga ccacagtgca acatgtaatt
     7861 ggttttctcg ggctttcttt acaatcgcat cattataact gccgccgacc ggccggaaaa
     7921 gctttggccg atttcctgtg atggcgtaaa ggatatcaga ggtttctttc atttcttgac
     7981 taagtctgct gagtttcatg gtgtgatcat aggtgtgatt gccgagttca tgcccttcca
     8041 ttacttgccg atggatgata tctgggaatt tttctgcgtg agctccgagc acaaagaagg
     8101 tagctgcggc ttcgtattca gctaatatat tgagaatggc aggagtgtat tttgaatgcg
     8161 gaccatcatc aaatgttaaa gcaattacct tttcctccgt ttttgcatcc cagaaaatat
     8221 ctccgtcttt ctcaaaatca aatctatctt tcgcatatat ctgctcttgg atgaatagaa
     8281 aagcacaaga agtcagaagc aggtaactaa tcgcataaaa tatttttttc ttcatttcac
     8341 acctctcatt ttcaaaaaat aagttaaact ttaagtattt atggacgtaa ttagttatta
     8401 attaaatcta ttatttgcta agaagcacgt ttttatcata agcaaatttt ggagaagaaa
     8461 agaggaaggc taaaccaact tcttcatttt aaatatactg taagaaaagc tgtataaaat
     8521 ggatgactta aaaacgcatg atggaaagat gcttccaaga aaaggttttg aaaagcagga
     8581 gcagaaaggt gaaaaaatta attactaaaa aggcaaaact taattaattc ctccaaaagt
     8641 ttagctataa aaagatacct gaatcaggat tactctccaa ggattcaggt atctgacaaa
     8701 aacagggctt aactatttgc ggttgcggtt attgcgtcgc tcgttgttgt ggttgttgtt
     8761 atcttcgaaa gcattctcgt cgataaattc attttcattg gcaaattcca cgttattgat
     8821 ttcttgatta ttgcggttct cattattgcg gtttttgcgt tcatttcggt taaagttacg
     8881 tttattgtta tccatggaaa gccctcccat tattctttat gagaaaaaca ttcatttttg
     8941 aagcgttact tgaagcagaa gcttccagta caaatgtcgt cctcgaatta tattttaccc
     9001 ccttatcaaa aattcactcg ttatttcgaa agccatattt tgttattttt taccgtccaa
     9061 acccattgga acggcaaggc tcggtgtgcc ggttaaatta actggaacaa atggcagaca
     9121 tcttctgata acttccagat tttgctctac ccattgttca ctatagatat gaggcgtgat
     9181 aggtatgttt ggaccgagca atatatcgac ctgtttaaaa gctttctcaa aacctttcac
     9241 caattgtctc cgggcctgct gcgcctgata tattgcggtg tttcagttaa agcgccggcc
     9301 agaaagaaac tccgtatgtc tgcagaatat tcgtttgaat gggtctggag ccattggtgg
     9361 tgtaaagcag ctgcttcgcc agccgctatc gtatagccgg aaaaggttga taaagctaat
     9421 tcaggaattt ctacatccac tatttcagca cctaaataga ggagtgtttc catggcatgt
     9481 ttgaaaagga tttctatatc aggggccagg tcttggagaa agtatgcagg ggtaccaatt
     9541 ttgattcctt ggattccatt atttaaatcg tatatataat ttggaacgga cacatccaga
     9601 cttcccggat tatttgcatc aaaacctgcc atataataaa gcatacatac taaatcagaa
     9661 acagatcttg ccatgggact ggcagtatct aaagtcatag ctgtaggaaa aataccatgc
     9721 gtactgacta atccataagt tgtcttcaga ccgtaaatac cgcagatgga agcgggtaag
     9781 cgaatgtatt aaaatgtatc tgatccagct gcaagctcat gcaaattttg ctttccgagc
     9841 atattgccgc ttgctcttac caatttatct ccggttgtcg cattttttta gaaataaaat
     9901 ctataaaaag tctggaatgg agtatattcc ggcgtaaatg gaaggtagac agagaccgcg
     9961 tctccctttg gacgttctct ttaatcatgc tgagattttc gaatataact ttcgatgcag
    10021 taatccaaag gaatgatgtt tttaggaagt acaagggatt tctcatattg tagtaaaaat
    10081 agcgttctaa tttaagaaaa attacgtaaa tatagaaatg gtatgtatac tgaaaggttt
    10141 gaaagggtga ttgtgtggat gagaaatcgt caagaaatca acattctgat aatagagatg
    10201 ttaagcagga ccaactagat cagtttcgcg taaaggattc agggaagccg atgacaacac
    10261 aggaagggaa aaagagatca caggaccagg accaattaaa ggctggtata cgcgggcctt
    10321 ccttacgtca ggattatgag ttttttgaaa aaatgtcgca ttttgtccat gaagaaatac
    10381 cggaaag
//
