LOCUS       NZ_CP076385            11314 bp    DNA     linear   CON 27-AUG-2024
DEFINITION  Streptomyces sp. G11C(2021) chromosome.
ACCESSION   NZ_CP076385
VERSION     NZ_CP076385.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. G11C(2021)
  ORGANISM  Streptomyces sp. G11C(2021)
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7137224)
  AUTHORS   Undabarrena,A., Serna,N., Zamora-Leiva,L., Cumsille,A., Gonzalez,V.,
            Valencia,R., Ugalde,J., Reyes,F., Olano,C., Salas,J.A. and Camara,B.
  TITLE     Towards deciphering Streptomyces albidoflavus metabologenomics:
            insights into a new member of the tetrahydroisoquinoline family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7137224)
  AUTHORS   Undabarrena,A., Serna,N., Zamora-Leiva,L., Cumsille,A., Gonzalez,V.,
            Valencia,R., Ugalde,J., Reyes,F., Olano,C., Salas,J.A. and Camara,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2021) Laboratorio de Microbiologia Molecular y
            Biotecnologia Ambiental, Universidad Tecnica Federico Santa Maria,
            Av. Espana 1680, Valparaiso 2340000, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024499195.1-RS_2024_08_27
            Annotation Date                   :: 08/27/2024 07:37:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,192
            CDSs (total)                      :: 6,097
            Genes (coding)                    :: 5,931
            CDSs (with protein)               :: 5,931
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 66 of 166
            Pseudo Genes (incomplete)         :: 127 of 166
            Pseudo Genes (internal stop)      :: 16 of 166
            Pseudo Genes (multiple problems)  :: 38 of 166
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:57:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1148779
            Orig. end                         :: 1160093
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP076385.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11314
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11314
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11314
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6314](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(186..1105)
                     /locus_tag="KNZ81_RS04665"
                     /old_locus_tag="KNZ81_04675"
                     /pseudo=""
     CDS             complement(186..1105)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998876.1"
                     /locus_tag="KNZ81_RS04665"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04675"
                     /product="sigma factor"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSGRTAGADPGEVTGERRQLLNGAYRLLGSLAEAEDAVQEAYARW
                     YALPDPRRDEIASPGAWLTTVTGRVCLDVLGSARARRERYVGAWLPDALPGHPGRPAGP
                     SYDPADQLVLDESVTMAFLVVLEAMTPAERVAFLLHDVFRYPVAEIAGVLDRTPAACNI
                     SPPPPGAGYGTAPPHRPADRPRPCGASRRRGRPRTSRLSSTSSTRPP"
     gene            1209..1584
                     /locus_tag="KNZ81_RS04670"
                     /old_locus_tag="KNZ81_04680"
                     /pseudo=""
     CDS             1209..1584
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641248.1"
                     /locus_tag="KNZ81_RS04670"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04680"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTSQATPRTTVRTHFSFLRTALALQALTIFVQGLGGAAAQHRVR
                     RTPARCRGAGDVRGVDAVPARRGPRLEAGRRLAEGRLARVRLPRAGLRPGGVGDRAPVL
                     APCAAGGADVRGESGGAGPAV"
     gene            1742..3106
                     /locus_tag="KNZ81_RS04675"
                     /old_locus_tag="KNZ81_04685"
     CDS             1742..3106
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="KNZ81_RS04675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04685"
                     /product="PhoX family protein"
                     /protein_id="WP_023416413.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLSELFAGTAAAKGMGKAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGQVPSNHDGMAAFGGRRGQVHLVRNHENR
                     PTAPIRVPPVRGLTYDPEGMGGCTALTLDPAHRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPVDPHRSGAVPLKAMGRFQHEAIAIDPRHGIVYE
                     TEDAFERPFGLFYRFLPEKPRGGLGSLRAGGALQAMRVPGVPDLSAIQETGASFDRVEW
                     VDVPDPLAEETPIRFQDFGPKGITHAQKLEGCYWGGRSVYFVSSFARSSEGSAADHYGQ
                     IWRYDPDRRRLTLVIVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVYGVTTR
                     GEVYPMARGRQNTGTPEEPAWGEFAGVCFSPDYDTMFVNCYTPGTTFAVTGPWHR"
     gene            3276..4145
                     /locus_tag="KNZ81_RS04680"
                     /old_locus_tag="KNZ81_04690"
     CDS             3276..4145
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="KNZ81_RS04680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04690"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_215222188.1"
                     /transl_table=11
                     /translation="MVRKAKLRTGTALGALMAVGAVAGCGQSSPDPHATAPGPRASHSA
                     SPAAPPAPPTLAPGPGGLAPVFANGPRNEGKTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRRLKVPATVFMTGRWAEEYPDQARAIGADPLFEIANHSYSHHAFTGDCYGLPRV
                     AESEMRADVERAYTAFRTAGVKKAMPYFRFPGGCYDDTALREVSAAGVTAVQWDVVSGD
                     AFATDAGAVAQQVLDGVRPGSVVVMHCTRSAAPATERALRRIVPALREQGYRFVKVSEL
                     IGAASRKG"
     gene            4176..4889
                     /locus_tag="KNZ81_RS04685"
                     /old_locus_tag="KNZ81_04695"
     CDS             4176..4889
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="KNZ81_RS04685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04695"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_164183218.1"
                     /transl_table=11
                     /translation="MTSEPLPDSPFRDERTARDEAPQFVLPLVVRLERAEPPALTDALQ
                     TAARAVLTLLSDPRSEGDGVWAEAVRDWQDARIRKVVRRARGAEWRKAGALPGITVEDG
                     GAEVRVYPPVPLDGWPRELAKLQVSGTELEDPVEPGEPAAGVPVLWLNPDVPMSAGKAM
                     AQAGHGAQLAWWQLSGAERNAWREQGFPLAVRTAGAERWKELVESGLPVVRDAGFTEIA
                     PGSCTVVAEHPALVG"
     gene            5001..6314
                     /locus_tag="KNZ81_RS04690"
                     /old_locus_tag="KNZ81_04700"
     CDS             5001..6314
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="KNZ81_RS04690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04700"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_164183216.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-94, bitscore: 314.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPAIAEAVEAMAGIEWVEVVAENVCPGHLPDSLRR
                     LRERGVTVVPHGVGLGLGGAERPDPVRLAALAERAEALDSPLVTEHIAFVRAGGERTAS
                     RGLEAGHLLPVPRTREGLAVLCENVRIAQDQLPVPLAVENIAALFSWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNQGEDPAAALDALPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPDPVLAVLAELASRVRPPGVLLERDERFPSAAGLEAEVAKIRRTVAAAGRDRVLV
                     GAGARPGSVPASGSPVADEAARTRLALGQTALLSALVAGTPAPEGFDRGRLRVQNRMLT
                     AKRGSVVASLAPELERILGDTYRPRFLRYAAGRPLTGGYRADALAFALHTLREEPPAER
                     AVRRALRDWWIERSGRASLVGRPWARWARVARFVLLGR"
     misc_feature    6437..6455
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.23"
                     /tool="antismash"
     gene            6438..8078
                     /locus_tag="KNZ81_RS04695"
                     /old_locus_tag="KNZ81_04705"
     CDS             6438..8078
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-479). E-value: 1e-21.
                     Score: 69.6. Matches aSDomain:
                     nrpspksdomains_KNZ81_RS04695_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="KNZ81_RS04695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04705"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_215222186.1"
                     /sec_met_domain="PF00561 (E-value: 5.2e-25, bitscore: 87.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLILTAAGALLLTPLAAAPQAPAAGAPAPSGGPEVRGVALAA
                     ERAARTPIAFGPCPDEEGLPRSLECGTVRVPLDYARPDGEQIDLTVSRAKAAGPKGERQ
                     GALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GSWQAPEHPTEKQKAGRRAEAEAYARGCAERGGKRLRHIHSLNNARDLDVVRASLGERK
                     LTFMGASYGSYFGALYAQLFPSHVRRMVFDSAVDPSPSRVWYRNNMAQSAAFEKRWADF
                     RGWIASHDAAYGMGTDAAQVLAGYRRAREKLAAEPAGGTVGPAQLQAVMLRAQYYEDYW
                     PSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECNDAPWPTSWETWDRD
                     HTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTLVLAGERDAATPYA
                     GALELRRRLPDAALVTERGAGTHGLAGGPNACVNRHVEAYLIDGKVPARSTDCAARPAP
                     EPEAQEAGTRAGGSGPALARAAGWPPAAR"
     aSDomain        6753..7874
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KNZ81_RS04695_Abhydrolase_1.1"
                     /evalue="1.00E-21"
                     /label="KNZ81_RS04695_Abhydrolase_1.1"
                     /locus_tag="KNZ81_RS04695"
                     /protein_end="479"
                     /protein_start="105"
                     /score="69.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGSWQAPEHPTEKQKAGRRAEAEAYARGCAERGGKRLRHIHSLNNAR
                     DLDVVRASLGERKLTFMGASYGSYFGALYAQLFPSHVRRMVFDSAVDPSPSRVWYRNNM
                     AQSAAFEKRWADFRGWIASHDAAYGMGTDAAQVLAGYRRAREKLAAEPAGGTVGPAQLQ
                     AVMLRAQYYEDYWPSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECND
                     APWPTSWETWDRDHTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTL
                     VLAGERDAATPYAGALELRRRLPDAALVTERGAG"
     gene            complement(8092..8808)
                     /gene="hemQ"
                     /locus_tag="KNZ81_RS04700"
                     /old_locus_tag="KNZ81_04710"
     CDS             complement(8092..8808)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="KNZ81_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04710"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_215222185.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDISGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARRHVREEVPF
                     YTGRRKSVADLVAELA"
     gene            complement(8813..10285)
                     /gene="hemG"
                     /locus_tag="KNZ81_RS04705"
                     /old_locus_tag="KNZ81_04715"
     CDS             complement(8813..10285)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="KNZ81_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KNZ81_04715"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_164183212.1"
                     /sec_met_domain="DAO (E-value: 2e-09, bitscore: 36.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETEGNPAGAGGASPRVVVVGGGVAGLTAALRLLDAGAEVTLL
                     EADERLGGKLRTGEIGGVRVDLGAESMLARRPEAVGLAREAGLAGALQPPATASASLWT
                     RDALRPMPKGHVMGVPADPASLAGVLSKEGVARIAEDRTLPRTEVGEDIAVGEYVATRV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAAREHTSLTEGVRAIQGRTAADPR
                     TDPVFMGIDGGVGRLPLAVADTVRARGGRIRTGTPVTGLARTPGGWRLTTGTPGAPEAL
                     EADAVVLAVPAPQAARLLAAELPAASADLAEIEYASMALMTFAFRAGEAELPQGSGFLV
                     PPVEGRTVKAATFAGRKWRWIAEQEPGLHVVRTSAGRHREEEILGRDDADLLALARRDL
                     KAATGVDAEPVDAHVTRWDQGLPQYPVGHLARVARVRDQVAKAPGLALCGAAYDGVGIP
                     ACVASATEAARQVLAPRREGDPASGSSGHAGE"
ORIGIN
        1 gggggtggct gccgacggtc agcgcgggga atccggcgtc ggccgccgtc cgcgcggacc
       61 agacgtccca gacggtcggc agcacgagcg gctgatggtc ggcgtgcagc tgctggaggc
      121 gctgagcgcg ctcgacggtg gttcgcaagt ccatgccggg gaagctaccc cccggatcgc
      181 cgcgatcaac gtgccgacgg ggcgccgtcc tccgcccagc cccgcagctt ctccggattg
      241 cggaccaccc agatgcgggc gacccggctg tcggcggcga ggtcgaaggc ggccacggtc
      301 aggacggtcc cggcgcggcg ggcgaccaga cccggggtgc cgttgaccgg acgttccagg
      361 agttcgaggc cgggggccct gtccgcgata ccggccatgt accgggcgat gtgagcgccg
      421 cccgccacgg ggcgcagggc ggcgccggcc aggccgccgc cgtcggcggt catcacggcg
      481 gccgggtcga ggaggtcgac gagagccgcg atgtccttgg cctcccacgc ctccttgacg
      541 cgccgcacgg tctcggcctg tccgccggcc ggtgcggggg agccgtcccg tacccggcgc
      601 cgggcggagg cggcgagatg ttgcaggcgg cgggggtccg gtcgaggaca ccggcgatct
      661 cggcgacggg gtagcggaag acgtcgtgga ggaggaacgc gacgcgttcg gccggggtca
      721 tcgcctccag gacgacgagg aaagccatgg tgacggactc gtccagcacc agctggtcgg
      781 cggggtcgta gctcggcccg gccggccggc cggggtggcc ggggagcgcg tcgggcagcc
      841 aggcgccgac gtagcgctcg cggcgggcgc gcgccgagcc gagcacgtcc aggcagacgc
      901 ggccggtcac cgtggtgagc caggcgccgg ggctggcgat ctcgtcccgg cgcgggtccg
      961 gcagcgcgta ccagcgggcg tacgcctcct ggacggcgtc ctcggcctcg gcgagtgaac
     1021 cgagcaaccg gtaggccccg ttcagcagtt ggcgccgttc gccggtgacc tcgccggggt
     1081 ccgccccagc cgtgcgaccg ctcacccgtc ccccctcgcc tgacctttcc ggggcccgtg
     1141 tcgtcgggct ggtgagacct ttatcgacat cggccgccgc cctcgggcgc ggaagagggg
     1201 gacccggcat gacgacgagc caggcgacgc cccgcacgac cgtccggacc cacttctcgt
     1261 tcctgcggac cgcactcgcc ctccaggccc tgacgatctt cgtgcagggt ctcggcgggg
     1321 ctgctgctca gcaccgggta cggcgaactc ctgcacggtg tcggggcgcg ggtgatgtac
     1381 ggggcgtcga tgctgtacct gctcgccgcg gtcctcgcct ggaagccggg cggcggctcg
     1441 ccgaaggccg tctggcacgc gtccggcttc ctcgtgctgg cctccgtcca ggtggtgtcg
     1501 gggatcgcgc acctgtcctc gctccatgtg ccgctggggg tgctgatgtt cggggcgagt
     1561 ctggcggcgc tggcccggcg gtgaggaccg ggccagcggc cgtaccccgg acccttcgtg
     1621 gcaggccagg tgggctgtga cggccgtgtc cagggccacg cctggttccg gtggggctgg
     1681 gtgcgtggta cacacgtgcg ggtcacattc ctgtccgcag agccgcagca gggagttgcc
     1741 catgtccgcg acacgccgtc aagttctcgc ccgtaccggt gccgtcgccg gtatcgcctt
     1801 caccggcagt ctctccgaac tcttcgcggg cacggccgcc gcgaagggga tggggaaggc
     1861 cgggtacggt ccgctggtgc ccgacccgaa gggcctgctg gacctgccga aaggtttccg
     1921 ctaccgggtg ctgtcccggg agggcgaccg gatgctctcc ggcgagggcc aggtgccgag
     1981 caaccacgac gggatggccg ccttcggcgg gcgccggggc caggtccacc tcgtccgcaa
     2041 ccacgagaac cggcccaccg ccccgatccg cgtcccgccc gtccgcgggc tgacgtacga
     2101 cccggagggg atgggcggct gcacggcgct caccctcgac cccgcgcacc gcgtcctcgg
     2161 cgagcgggtc gccatcgccg gtacctcgac caactgcgcc ggcgggccga ccccctgggg
     2221 cacctggctc acctgcgagg agaccgagga caaggccggg accaacggct acaccaagga
     2281 tcacggcttc atcttcgagg tcgacccggt cgacccgcac aggagcggcg cggtgccgct
     2341 gaaggcgatg ggccgcttcc agcacgaggc gatcgcgatc gacccgcgcc acgggatcgt
     2401 ctacgagacc gaggacgcct tcgagcggcc gttcgggctc ttctaccgct tcctgcccga
     2461 gaagccgcgc ggcggcctcg gctcgctgcg ggccgggggc gcgctccagg cgatgcgggt
     2521 gcccggggtg cccgacctct cggcgatcca ggagacgggc gcctcgttcg accgggtgga
     2581 gtgggtggac gtgccggacc cgctcgccga ggagacgccg atccgcttcc aggacttcgg
     2641 cccgaagggc atcacccatg cccagaagct ggagggctgc tactggggcg gccgctcggt
     2701 ctacttcgtc tccagcttcg cccggagcag cgagggctcg gccgccgacc actacgggca
     2761 gatctggcgc tacgacccgg accggcggcg cctcaccctg gtcatcgtct tcggccccga
     2821 caccgatgtg cagctcccgg gcgaggagcc ggacaacatc tgcctcgccc cgagcggcgg
     2881 cctgatggtc gccgaggacg gcgagggcgc ccagcacgtc tacggcgtga ccacgcgggg
     2941 cgaggtctat ccgatggcgc ggggccgcca gaacacgggc acgcccgagg agccggcgtg
     3001 gggcgagttc gcgggcgtct gcttctcccc ggactacgac acgatgttcg tcaactgcta
     3061 cacaccgggg acgaccttcg cggtcaccgg gccctggcac cggtgacgtg agcgggggcg
     3121 gggccgggcc gtcggcctcg ccccccatct cgcgggcccg gccaggcacc cggacggcgg
     3181 aaggcggcgg ggcgagacgg gtgaggccac accccccggc cggcattttg cccgatattc
     3241 ggcttttcgc atcctagggt cacggcgtga tcagcatggt gcgcaaggcg aagctccgga
     3301 ccggcacggc gctgggcgcc ctcatggcgg tgggcgcggt ggccgggtgc ggccagtcct
     3361 ccccggaccc ccacgccacg gcaccggggc cccgggcctc gcactccgcc tccccggccg
     3421 ccccgccggc gccgcccacg ctggcgcccg gccccggcgg cctcgccccg gtcttcgcca
     3481 acgggccccg gaacgaaggc aagaccgtcg cgctcacctt cgacgccgac atgacggccg
     3541 accagggcga gcgcgcggcc ggcggcgagc gcttcgacca cccggagctg atcgcctcgc
     3601 tgcgccgcct gaaggtgccc gccaccgtct tcatgaccgg acggtgggcc gaggagtacc
     3661 cggaccaggc gcgggccatc ggcgccgacc cgctcttcga gatcgccaac cactcctaca
     3721 gccaccacgc cttcaccggt gactgctacg gcctgccgcg ggtcgccgag agcgagatgc
     3781 gggccgacgt ggaacgggcg tacaccgcgt tccgcacggc cggggtgaag aaggcgatgc
     3841 cgtacttccg cttccccggc gggtgttatg acgacacggc gctgcgggag gtctcggcgg
     3901 ccggggtgac ggcggtgcag tgggacgtgg tgagcgggga cgccttcgcc accgacgccg
     3961 gcgcggtggc gcagcaggtg ctggacgggg tgcgccccgg ctcggtcgtg gtcatgcact
     4021 gcacccgcag cgccgccccc gccaccgagc gcgcgctgcg ccggatcgtg cccgccctgc
     4081 gcgagcaggg gtaccgcttc gtgaaggtgt ccgagctgat cggggcggct tcgcggaagg
     4141 gatgagggag tctcgccgtt tggcagactg gctccgtgac cagcgaaccc ctccccgaca
     4201 gtcccttccg tgacgagcgc accgcccgcg acgaggcacc gcagttcgta ctgccgctcg
     4261 tcgtccggct ggagcgcgcc gagccgcccg ccctcaccga cgcgctccag acggccgccc
     4321 gggccgtcct gacgctgctc agcgatcccc gctcggaggg ggacggcgtc tgggcggagg
     4381 cggtgcggga ctggcaggac gcgcgtatcc gcaaggtggt gcggcgggcc cggggcgcgg
     4441 agtggcgcaa ggccggggcc ctgccgggga tcacggtcga ggacggcggc gccgaggtgc
     4501 gggtctaccc gccggtgccg ctggacggct ggcccaggga gctggccaag ctccaggtct
     4561 ccggcaccga gctggaggac cccgtggagc cgggcgagcc cgcggccggc gtcccggtgc
     4621 tctggctcaa cccggacgtg cccatgtcgg cggggaaggc gatggcgcag gcgggccacg
     4681 gcgcgcaact ggcctggtgg cagctgtcgg gggcggaacg caacgcctgg cgcgagcagg
     4741 ggttcccgct ggcggtgcgg accgccggcg cggagcgctg gaaggagctg gtggagagcg
     4801 ggctgccggt ggtgcgggac gccgggttca cggagatcgc gcccgggtcg tgcacggtgg
     4861 tggcggagca tcccgcattg gtggggtgac ggcggcgaag ctcagatcct gctctgaagc
     4921 gggcggatga cggttccggg acggacggcg gcgggcaggg tggccgcagg gaccgggggg
     4981 tccctggagg gggtggcggt atggaacggc tcggaacggg gatcggctgg cggcccgcga
     5041 tcgcggaggc cgtcgaggcg atggcgggga tcgagtgggt ggaggtggtg gcggagaacg
     5101 tctgccccgg tcaccttccc gactccctgc ggcggctgcg ggagcgtggg gtgaccgtgg
     5161 tgccgcacgg ggtggggctg ggactgggtg gtgcggagcg gcccgatccg gtgcggctgg
     5221 cggcgctggc cgagcgggcc gaggcgctgg actcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtcagggc gggcggggag cggaccgcgt cgcgcgggct ggaggccggg cacctgctgc
     5341 cggtcccccg gacccgggaa gggctcgcgg tgctctgcga gaacgtgcgg atcgcccagg
     5401 accagctgcc cgtgccgctc gccgtggaga acatcgcggc gctcttctcc tggccgggcg
     5461 aggagctgac ggaggggcag ttcctctacg agctggccga ccggaccggg gtgcggctgc
     5521 tgatcgacgt cgccaacctg cacaccaacc acgtcaacca gggcgaggac cccgccgccg
     5581 ccctcgacgc gctgccgctg gaggcgctgg cgtacgtcca tgtggcgggc gggttcgagc
     5641 gggacggggt gtggcacgac agccacgccc acccggtgcc ggatccggtg ctggcggtct
     5701 tggccgagct ggcctcgcga gtgcggccgc cgggggtgct gctggagcgg gacgagcggt
     5761 tcccgtctgc ggccgggctg gaggcggagg tcgcgaagat ccggcggacg gtggcggcgg
     5821 cgggccggga ccgggttttg gtgggagcgg gggcgcggcc cgggtccgta ccggcgtcgg
     5881 gcagcccggt ggcggacgag gcggctcgga cgcggctggc cctcgggcag acggcgctgc
     5941 tttcggcgct ggtcgcgggg actccggcac cggaggggtt cgaccggggc cggctgcggg
     6001 tgcagaaccg gatgctgacg gccaagcggg gttccgtcgt ggcctccctc gccccggagc
     6061 tggagcggat cctgggcgac acgtaccggc cgcgcttcct gcggtacgcg gcgggccggc
     6121 cgctgaccgg cgggtaccgc gcggacgcgc tcgccttcgc gctgcacacc ttgcgggagg
     6181 aaccgccggc cgagcgggcg gtccggcgcg cgctgcgcga ctggtggatc gagcgctcgg
     6241 gccgcgcgtc cctggtgggc aggccctggg cccggtgggc cagggtggcg cgcttcgtgc
     6301 tgctggggcg ctgagaccgg cggccgtgtt gggccgatcc gagcaggtct gtccaacacg
     6361 cgccccggtg cttgcccttg ccgatcggcc tgcgaatcat ccctagggcc tgtcatcggc
     6421 cggacgaaag ggacgcgatg cgagcgccgc tcatcctcac cgccgccgga gccctgctcc
     6481 tcaccccgct cgcggctgcc ccgcaggctc ccgcggccgg tgctccggcc ccgtccggcg
     6541 gccccgaggt gcgcggggtg gcgctcgcgg ccgagcgggc ggcgcggacc ccgatcgcct
     6601 tcgggccgtg cccggacgag gaggggctgc cccggtcgct ggagtgcggc acggtacggg
     6661 tgccgctgga ctacgcgcgg ccggacggcg agcagatcga cctgaccgtg agccgggcca
     6721 aggcggcggg cccgaagggc gagcggcagg gcgcgctggt cttcaacccg ggtggtcccg
     6781 gctcctcggg gatgctcttc ccgctggtcg ggatcttccc cgagtggaag cggctggcct
     6841 cggtctacga cctggtcggg tacgcgccgc gcggggtcgg ccggtcggcg ccgctctcct
     6901 gccgggaccc gcgcgagaag ccggacggga gctggcaggc ccccgagcac ccgaccgaga
     6961 agcagaaggc cgggcgcagg gccgaggccg aggcgtacgc gcgggggtgc gccgagcgcg
     7021 gcgggaagcg gctgcggcac atccactcgc tgaacaacgc ccgcgacctg gacgtggtgc
     7081 gggccagcct cggtgagcgc aagctgacgt tcatgggcgc gtcgtacggc tcgtacttcg
     7141 gggcgctgta cgcgcagctc ttcccctcgc acgtgcgcag gatggtgttc gactcggcgg
     7201 tcgacccctc gccctcgcgc gtctggtacc ggaacaacat ggcgcagtcg gcggcgttcg
     7261 agaagcggtg ggcggacttc cgggggtgga tcgcctcgca cgacgcggcg tacgggatgg
     7321 gtaccgacgc ggcccaggtg ctggcggggt accggcgggc gcgcgagaag ctggcggccg
     7381 agcccgccgg gggcacggtc ggcccggcgc agctccaggc ggtgatgttg cgggcccagt
     7441 actacgagga ctactggccc tcgcacgcgg aggcgctcgc ggcgtacctg aagggtgatc
     7501 cgcagccgct ggtcaatctg gcgaccccgc tggaggaggg ccgggtggag gcggagaacg
     7561 cgcgcgccgt ctacacggcg gtggagtgca acgacgcgcc gtggcccacc agttgggaga
     7621 cctgggaccg cgaccacacc cgtctcgccc gcacggcgcc gttcgagacc tgggagaacg
     7681 cctggatgaa cctgccgtgc gcgtactgga aggagccccg gcagacgccg atggacgtgc
     7741 gggtggcccc cggtgagctg ccgccgacgc tggtcctcgc cggggaacgg gacgcggcaa
     7801 ccccgtacgc gggcgcgctg gagctgcggc ggcgcctgcc ggacgccgcg ctggtgaccg
     7861 aacggggcgc gggcacgcac gggctggcgg gcgggccgaa cgcctgcgtc aaccggcacg
     7921 tggaggccta cctgatcgac gggaaggtcc ccgcgcgctc caccgactgc gctgcccggc
     7981 cggcccccga gccggaggcg caggaagccg ggacgcgggc gggcgggagc ggaccggcgc
     8041 tcgcccgggc cgccgggtgg cccccggcgg cccggtgagg cggagcgggg ctcaggcgag
     8101 ctcggcgacc aggtcggcga cggacttgcg gcgcccggtg tagaagggca cctcctcgcg
     8161 gacgtggcgg cgggcctcgg aggcgcgcag gtggcgcatg aggtcgacga tgcggtacag
     8221 ctcgtcggcc tcgaaggcga gaatccactc gtagtcgccg agggagaacg aggcgaccgt
     8281 gttggcgcgg acgtccgggt agccgcgcgc catcttgccg tggtcggcga gcatcgcccg
     8341 gcgctcctcg tcgggaagga ggtaccactc gtaggagcgg acgaacgggt agacgctgat
     8401 gtagtcgcgg ggcgtctcgt cggcgaggaa cgccgggatg tgcgacttgt tgaactcggc
     8461 gggccggtgc agcgccatgt tcgaccagac cggctccaga gcgcgcccga ggcgggtgcg
     8521 gcggaagagg ttgtacgcct cctggagggc gtcggcggtc tcggcgtgcc accagatcat
     8581 cacgtcggcg tcggcgcgca gaccggagat gtcgtaggtg ccgcggacgg tgacgtcctt
     8641 ggcggcgagc ttgtcgaaca ggtcctggac ctcgtcggcg tagccggacc ggtcctcggg
     8701 gagcacgtcg cggagcttga agacggacca cagggtgtag cggatgacct cgttgaggtc
     8761 cttggccttc ttgcccgcgt tggggatctt ctcgggagca gcactcatgg ggtcattctc
     8821 ccgcgtgccc ggacgagccc gacgccgggt cgccttcccg gcggggcgcg aggacctggc
     8881 gcgccgcttc ggtggcggag gcgacgcagg cggggatgcc gacgccgtcg taggcggcgc
     8941 cgcacagggc gaggccgggg gccttggcga cctggtcgcg gacgcgggcg acgcgggcga
     9001 ggtggccgac cgggtactgg ggcaggccct ggtcccagcg ggtgacgtgg gcgtcgaccg
     9061 gctcggcgtc gaccccggtg gcggccttga ggtcgcgacg ggcgagggcg aggaggtcgg
     9121 cgtcgtcacg gccgaggatc tcctcctcgc ggtggcggcc ggccgaggtg cggacgacgt
     9181 gcagccccgg ctcctgctcg gcgatccacc gccacttgcg gcccgcgaag gtggcggcct
     9241 tgacggtacg tccctcgacc ggcggcacga ggaagccgct gccctgcggc agctccgcct
     9301 cgcccgcgcg gaaggcgaag gtcatcaggg ccatcgaggc gtactcgatc tcggcgaggt
     9361 cggcggaggc ggccgggagt tcggcggcga gcagccgggc cgcctggggt gcgggtacgg
     9421 cgaggaccac ggcgtcggcc tccagggcct cgggcgcgcc gggggtgccg gtggtcagcc
     9481 gccagccgcc gggggtgcgg gcgaggccgg tgacgggcgt gccggtgcgg atgcggccgc
     9541 cgcgggcgcg gacggtgtcg gcgacggcga gcgggaggcg gccgaccccg ccgtcgatgc
     9601 ccatgaagac cgggtcggtg cgcgggtcgg cggcggtgcg gccctggatc gcgcggacgc
     9661 cctcggtcag ggaggtgtgc tcgcgcgcgg cggcgaagag ctgggggacg gcggcgcgca
     9721 gcgagatgcg gtacgcgttg ccggcgtaga cgccgccgag gaggggttcg acgagccggt
     9781 cgacgacctc acggccgacc cgggtggcga cgtactcgcc gacggcgatg tcctcgccga
     9841 cctcggtgcg gggcagggtg cggtcctcgg cgatccgcgc gacgccctcc tttgagagga
     9901 cccctgcgag gctggccggg tcggcgggga cgcccatgac gtggcccttg ggcatggggc
     9961 gcagggcgtc gcgggtccac agggaggcgc tggcggtggc ggggggctgg agggcgccgg
    10021 ccagtcccgc ctcacgggcg aggccgaccg cctcggggcg gcgggccagc atcgactcgg
    10081 cccccaggtc gacgcggacg ccgccgatct cgccggtgcg gagcttgccg ccgagccgct
    10141 cgtcggcttc caggagggtc acctcggccc cggcgtcgag caggcgcagc gcggccgtga
    10201 gccccgcgac gccgcctccg acgaccacca cacgggggct cgcgccaccc gctcccgccg
    10261 ggtttccctc tgtttccgcg cgcatggagc cactgtctca aaccggacgc ggcgggcggc
    10321 cgccggggca gcgcggttcc gttactcgtt cgcgacccgg gaggcgaaac ccaccggtgc
    10381 gccgggccgt cgtagcggca cgggcgcccc ggccggggcg cccgaaggac acaagggggg
    10441 cggcggatga gccggatgcg gggaacgagc ggcggccgga tcgtggggcg gcaggcggcg
    10501 gtggtgctgg cggcggcggt actgggtctc gcggggtgtt cggccgggcc ggactccggg
    10561 gcgtcgccgg cgggggaccg tgcggccgcg ccgtcggcgg ccgacggcgc gggcggcggt
    10621 caggccgaca acgcgaagcg ggagtcggcg gacgaggccg cggacgggaa ggcgcgcccc
    10681 gagctgaagg gcgtccacct gatccgtacg gccgagctgc atctgcgggt gaaggacgtg
    10741 ccctcggcgc tggcctcggc ccggtcggtg gtcgagggcg cgggcggcta cgtcggcaac
    10801 gagtcgaccc ggcgcgactc gcagggccac gagcggtcga ccgtggtggt ccgcgtcccg
    10861 caggagcggt acgaggcggt gctggactcg ctcggcgagg acggcgaggt ggtctcccgg
    10921 cggaccagcg cggaggacgt caccgaggag gtggtggacg tcgagagccg gctgaagtcg
    10981 cagcgggaca gcgtggcgcg ggtccgggag ctgatggacc gggcggagaa gctgagcgac
    11041 atcgtctcgc tggagggcga gctgagcagc cgccagatgg agctggaggc gttgctggcc
    11101 cagcagtcct cgctgaagga ccgcacctcg atggcgacgg tcaccctcca gctgaccgag
    11161 cggacggcga agacggagcc gaaggaggac gacggcccgg ccttcaccga cgcgctctcc
    11221 ggcggctggt cggcgttcgt ggcgacgctg cgctggctga ccgtggtggt cggggtggcg
    11281 ctgcctttcg tcctcaccct cgggctggcc ctgc
//
