GCF_024496245.1 - 8 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP101740.1 (Sphingomonas qomolangmaensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	terpene-precursor	93,222	114,121
Region 2	t3nrps-iterative	618,990	679,727
Region 3	T3PKS,terpene	2,542,997	2,606,658	Low	carotenoid	terpene
Region 4	NRPS,other	2,927,907	3,006,082
Region 5	lassopeptide	3,068,032	3,090,497
Region 6	lassopeptide	3,153,588	3,178,086	Low	ambactin	NRPS:Type I
Region 7	azole-containing-RiPP,lassopeptide	3,215,062	3,256,951
Region 8	hserlactone	3,279,013	3,299,618

NZ_CP101740.1 - Region 1 - terpene-precursor

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Location: 93,222 - 114,121 nt. (total: 20,900 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

NMP03_RS00560: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Ligases: ligase synthase synthetase

NMP03_RS00580: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

NMP03_RS00590: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

NMP03_RS00620: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000307.4	0.56	NRPS	AbT1	Aureobasidium pullulans
BGC0001160.5	0.52	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0000779.3	0.45	saccharide	lipopolysaccharide	Aeromonas piscicola
BGC0002405.2	0.45	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001137.5	0.42	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0002711.2	0.41	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000307.4	0.56	NRPS	AbT1	Aureobasidium pullulans
BGC0001160.5	0.52	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0000779.3	0.45	saccharide	lipopolysaccharide	Aeromonas piscicola
BGC0002405.2	0.45	saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001137.5	0.42	other (other)	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans
BGC0002711.2	0.41	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

NMP03_RS00580: PT_FPPS_like

Domain 1 (37 - 298): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.70 out of 23.79

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.28 out of 19.52

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

Additional weak hits (9):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.90 out of 26.12

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.37 out of 32.74

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.19 out of 24.78

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.47 out of 30.98

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.61 out of 33.14

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.73 out of 27.04

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.86 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism

NZ_CP101740.1 - Region 2 - t3nrps-iterative

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Location: 618,990 - 679,727 nt. (total: 60,738 nt) Show pHMM detection rules used

t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

NMP03_RS03065: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

NMP03_RS03215: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: aminotransferase glycosyltransferase

NMP03_RS03130: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

NMP03_RS03180: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

NMP03_RS03185: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

Hydrolases: deacetylase hydrolase

NMP03_RS03120: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

NMP03_RS03140: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

NMP03_RS03145: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: cyclase decarboxylase

NMP03_RS03090: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

NMP03_RS03155: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

NMP03_RS03160: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

NMP03_RS03250: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

NMP03_RS03255: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Ligases: ligase synthase

NMP03_RS03085: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

NMP03_RS03165: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002382.3	0.48	ribosomal	grimoviridin	Grimontia marina
BGC0000885.3	0.46	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001374.5	0.45	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001880.3	0.44	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0000254.5	0.44	PKS	oxytetracycline	Streptomyces rimosus
BGC0001369.3	0.43	PKS	metatricycloene	uncultured bacterium
BGC0000694.3	0.43	saccharide	butirosin	Bacillus circulans
BGC0001591.4	0.43	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002499.2	0.42	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0000781.3	0.42	saccharide	O-antigen	Pseudomonas aeruginosa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002382.3	0.48	ribosomal	grimoviridin	Grimontia marina
BGC0000885.3	0.46	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001374.5	0.45	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001880.3	0.44	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0000254.5	0.44	PKS	oxytetracycline	Streptomyces rimosus
BGC0001369.3	0.43	PKS	metatricycloene	uncultured bacterium
BGC0000694.3	0.43	saccharide	butirosin	Bacillus circulans
BGC0001591.4	0.43	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002499.2	0.42	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0000781.3	0.42	saccharide	O-antigen	Pseudomonas aeruginosa

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 23.52 out of 26.33

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.67 out of 25.88

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.82 out of 21.84

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.77 out of 29.72

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

Additional weak hits (14):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.05 out of 33.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.41 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.20 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.62 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.32 out of 26.12

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.67 out of 29.38

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.24 out of 25.88

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.52 out of 18.57

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.30 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.04 out of 21.55

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.88 out of 29.65

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

NMP03_RS03085: X

AMP-binding (16..406): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GSVLFDYGQNQSAGTNSGGYPMYGLTEA-FRSTY
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
MexT	Global virulence regulator
NtrC	Nitrogen regulatory protein C
PsrA	Oleic acid responsive regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP101740.1 - Region 3 - T3PKS,terpene

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Location: 2,542,997 - 2,606,658 nt. (total: 63,662 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

NMP03_RS12225: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

NMP03_RS12385: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

NMP03_RS12425: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Transferases: aminotransferase

NMP03_RS12295: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

NMP03_RS12305: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Isomerases: isomerase

NMP03_RS12300: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: synthase

NMP03_RS12235: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

NMP03_RS12325: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000656.5	0.44	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000647.3	0.38	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000630.3	0.25	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000640.3	0.25	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000638.3	0.24	terpene	carotenoid	Pantoea ananatis
BGC0000641.3	0.24	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.24	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.22	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000635.3	0.22	terpene	carotenoid	Paracoccus sp. N81106
BGC0000646.5	0.21	terpene	β-carotein	uncultured bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000656.5	0.75	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000647.3	0.70	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000630.3	0.62	terpene	(2R,3S,3'S)-2-hydroxyastaxanthin	Paracoccus haeundaensis
BGC0000640.3	0.61	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000638.3	0.60	terpene	carotenoid	Pantoea ananatis
BGC0000641.3	0.59	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000639.3	0.59	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000642.3	0.58	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000635.3	0.58	terpene	carotenoid	Paracoccus sp. N81106
BGC0000646.5	0.56	terpene	β-carotein	uncultured bacterium

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

NMP03_RS12375: PT_phytoene_like

Domain 1 (19 - 311): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → diapophytoene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

NMP03_RS12390: Lycopene_cycl

Domain 1 (9 - 379): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.12 out of 20.97

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

Additional weak hits (25):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.96 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.41 out of 20.97

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.11 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.24 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.27 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.44 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.58 out of 31.73

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.90 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.46 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.33 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.33 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.47 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.88 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.04 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.72 out of 29.65

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.17 out of 33.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.85 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.53 out of 26.06

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A

NZ_CP101740.1 - Region 4 - NRPS,other

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Location: 2,927,907 - 3,006,082 nt. (total: 78,176 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

NMP03_RS14185: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

NMP03_RS14195: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

Transferases: PPTase acyltransferase aminotransferase

NMP03_RS14055: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

NMP03_RS14110: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

NMP03_RS14135: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

NMP03_RS14170: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: deaminase dehalogenase hydrolase

NMP03_RS14125: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

NMP03_RS14140: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

NMP03_RS14205: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Lyases: decarboxylase

NMP03_RS14160: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Isomerases: mutase

NMP03_RS14095: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

NMP03_RS14065: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

NMP03_RS14130: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

NMP03_RS14155: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

NMP03_RS14175: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

NMP03_RS14200: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.94	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.89	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.88	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.87	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.86	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.83	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.82	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.81	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.80	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.79	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.78	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.78	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.76	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.73	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.73	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.86 out of 21.84

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.67 out of 25.31

Confidence: medium

Additional weak hits (19):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.89 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.00 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.37 out of 18.57

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.44 out of 36.54

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.93 out of 31.73

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.04 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.52 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.34 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.06 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.36 out of 24.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.67 out of 36.53

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.27 out of 39.62

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.55 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.09 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.44 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 6, location 2927906 - 3006082:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - Pro) + (Asp) + (NH2 - X - X - Leu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 2927906 - 2999492:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - Pro) + (Asp) + (NH2 - X - X - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

NMP03_RS14130: X

AMP-binding (505..906): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ATLGFDISIFDMTLVCGGEINAYGPTETTIWATY
Nearest Stachelhaus code(s):: Ph-Ser or Phe DAFTVAAIWK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

NMP03_RS14135: X - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (492..827): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IDPPGDRAAMVGTALSLREVQ---PLRAIADAAA
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1517..1919): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVSFQEAFLVAAGEYNQYGPTESHVVTEA
Nearest Stachelhaus code(s):: Pro DVQFVAQVVK (85% 8Å match); Hpr DVQFVAQVVK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

NMP03_RS14155: X

AMP-binding (19..406): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SAFLFQQRLMYAIARAAGK----------IFAKV
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

NMP03_RS14170: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (483..882): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YAVQVDSSNTMPKIVLGGEYIHYGPTETTVGVLT
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

NMP03_RS14175: NH2 - X - X - Leu

Search NORINE for peptide: strict or relaxed

CAL_domain (37..480): NH2

minowa: NH2

Prediction, score:

NH2: 204.4
Acetyl-CoA: 40.1
fatty_acid: 16.2
AHBA: 14.5
shikimic_acid: 1.8

AMP-binding (1145..1552): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAKAFDISVWQAEILLTGEINAYGPTECSDDVTH
Nearest Stachelhaus code(s):: Dpr DIWELTADDK (88% 8Å match); Dab DIWELTADDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2209..2607): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFVDSIWEGVLTVSGELNLYGCSEASGDSTA
Nearest Stachelhaus code(s):: bAla VDWVTSLADK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (3260..3655): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: CWMTFDPSVWEWYFICGGEHHSYGPTEASIAASE
Nearest Stachelhaus code(s):: Glu DVWHIGSIGK (76% 8Å match); Aad or Glu DVDHIGSIAK (44% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CatR	H2O2-responsive repressor
MexT	Global virulence regulator

NZ_CP101740.1 - Region 5 - lassopeptide

Download region GenBank file

Download region SVG

Location: 3,068,032 - 3,090,497 nt. (total: 22,466 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

NMP03_RS14570: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Ligases: synthase

NMP03_RS14600: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002312.2	0.52	ribosomal	ubonodin	Burkholderia ubonensis
BGC0001356.4	0.50	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0001496.3	0.47	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0001639.3	0.46	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000740.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000741.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002312.2	0.52	ribosomal	ubonodin	Burkholderia ubonensis
BGC0001356.4	0.50	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0001496.3	0.47	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0001639.3	0.46	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000740.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000741.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for NMP03_RS14555

NMP03_RS14555

Leader:

MELLGSFETLTK

Full core:

GGATGNALDAAFPTGTPFSELTFS

Putative macrolactam:

GGATGNALD

antiSMASH-DB 4.0 matches for NMP03_RS14555

100.0%

NZ_CP101740.1:

Lassopeptides: NMP03_RS14555_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GGATGNALDAAFPTGTPFSELTFS

1

GGATGNALDAAFPTGTPFSELTFS

24

75.0%

NZ_CP034767.1:

Lassopeptides: ELX51_RS02725_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

G +TG+ALDA FPTGTPF +LTFS

1

GASTGSALDATFPTGTPFGDLTFS

24

70.8%

NC_015730.1:

Lassopeptides: RLO149_RS23420_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GG+TG++LDAAFP GTPF++LT S

1

GGSTGSSLDAAFPAGTPFADLTLS

24

with 88 more sequence matches

Only the first 10 are displayed here.

70.8%

NZ_CP009122.1:

Lassopeptides: SKP52_RS26305_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GG++G+ LDA FP+GTPFS+LTFS

1

GGSSGSNLDADFPSGTPFSDLTFS

24

70.8%

NZ_CP020612.1:

Lassopeptides: allorf_0899068_0899265_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

G +TGNALD FP GTP S+LTFS

1

GASTGNALDRQFPVGTPKSQLTFS

24

70.8%

NZ_CP027407.1:

Lassopeptides: CEP88_RS08565_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GG+TG++LDAAFP GTPF++LT S

1

GGSTGSSLDAAFPAGTPFTDLTLS

24

70.8%

NZ_CP034444.1and 2 others:

NZ_CP034447.1 Lassopeptides: EJ070_RS06315_lassopeptideNZ_CP034451.1 Lassopeptides: EJ074_RS13805_lassopeptide

Lassopeptides: EJ067_RS13990_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GGA ALDAAFP TPF +LTFS

1

GGAGTTALDAAFPAHTPFDQLTFS

24

70.8%

NZ_CP034455.1:

Lassopeptides: EJ078_RS12825_lassopeptide

2

GATGN-ALDAAFPTGTPFSELTFS

24

GA+G ALDAAFP TP ELTFS

1

GASGTTALDAAFPAHTPVGELTFS

24

70.8%

NZ_LMIB01000001.1:

Lassopeptides: ASE00_RS23305_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GGA G LDAAF +GTP S+LTFS

1

GGADGTVLDAAFSSGTPVSDLTFS

24

70.8%

NZ_VDES01000002.1:

Lassopeptides: FG486_RS06170_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

G +TG+A+DA FP GTPFS+LTFS

1

GASTGSAIDANFPVGTPFSQLTFS

24

66.7%

NZ_AP023247.1:

Lassopeptides: JG743_RS01195_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GG+ ALDAAFP TPF +LTFS

1

GGSGTTALDAAFPAHTPFDQLTFS

24

66.7%

NZ_AP024109.1and 2 others:

NZ_CP083956.1 Lassopeptides: FJ974_RS01165_lassopeptideNZ_FOVT01000001.1 Lassopeptides: F3H96_RS01735_lassopeptide

Lassopeptides: MJ8_RS02965_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GGA ALDA+FP TP ELTFS

1

GGAGTTALDASFPAHTPVGELTFS

24

66.7%

NZ_ATYO01000004.1:

Lassopeptides: YU3_RS40500_lassopeptide

1

GGATGNALDAAFPTGTPFSELTFS

24

GGA ALDA+FP TP ELTFS

1

GGAGVTALDASFPAHTPVGELTFS

24

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.75 out of 18.57

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.12 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 20.47

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.99 out of 27.55

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.64 out of 29.72

Confidence: medium

Additional weak hits (13):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.62 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.76 out of 30.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 24.73 out of 32.74

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.58 out of 30.22

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.24 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.44 out of 29.65

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.78 out of 39.62

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 23.44 out of 30.12

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.26 out of 27.18

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.86 out of 27.18

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 22.86 out of 33.14

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.44 out of 36.53

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

NMP03_RS14555 - Class II: Cleavage pHMM score: -0.60; RODEO score: 23; Monoisotopic mass: 2310.1 Da; Molecular weight: 2311.5 Da; Number of bridges: 0

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 8 - 87; Score: 69.1

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP101740.1 - Region 6 - lassopeptide

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Location: 3,153,588 - 3,178,086 nt. (total: 24,499 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

NMP03_RS15030: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

Transferases: glycosyltransferase sulfotransferase

NMP03_RS14960: Sulfotransferase domain

extras: Sulfotransfer_1: Sulfotransferase domain

NMP03_RS15025: sugar transferase

smcogs: SMCOG1146: sugar transferase

NMP03_RS15035: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: hydrolase

NMP03_RS14985: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.48	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0000742.3	0.47	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000743.3	0.47	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000800.5	0.44	saccharide	xanthan	Xanthomonas campestris
BGC0000741.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000740.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001356.4	0.48	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0000742.3	0.47	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000743.3	0.47	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000800.5	0.44	saccharide	xanthan	Xanthomonas campestris
BGC0000741.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000740.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.43	saccharide	capsular polysaccharide	Streptococcus agalactiae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.12 out of 30.22

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.64 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.21 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.07 out of 28.19

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 1 - 84; Score: 63.3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_CP101740.1 - Region 7 - azole-containing-RiPP,lassopeptide

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Location: 3,215,062 - 3,256,951 nt. (total: 41,890 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

NMP03_RS15325: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.27	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0000573.3	0.24	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0000576.3	0.23	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0001786.3	0.22	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000580.3	0.20	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000570.3	0.20	ribosomal	astexin-1	Asticcacaulis excentricus CB 48
BGC0000574.3	0.18	ribosomal	caulosegnin I, caulosegnin II, caulosegnin III	Caulobacter segnis ATCC 21756
BGC0000568.5	0.15	ribosomal	microcin B17	Escherichia coli
BGC0001174.4	0.14	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0002115.3	0.14	ribosomal	streptamidine	Streptomyces albidoflavus J1074

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000577.3	0.61	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0000573.3	0.59	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0001786.3	0.56	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000576.3	0.56	ribosomal	rubrivinodin	Rubrivivax gelatinosus IL144
BGC0002150.2	0.53	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.53	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0000580.3	0.52	ribosomal	xanthomonin II, xanthomonin I	Xanthomonas gardneri ATCC 19865
BGC0000570.3	0.52	ribosomal	astexin-1	Asticcacaulis excentricus CB 48
BGC0000574.3	0.50	ribosomal	caulosegnin I, caulosegnin II, caulosegnin III	Caulobacter segnis ATCC 21756
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for NMP03_RS15320

NMP03_RS15320

Leader:

MRIEDTNTIELGVASTDTR

Full core:

GSTMGFSDGETGLMWHPGLYDD

Putative macrolactam:

GSTMGFSD

antiSMASH-DB 4.0 matches for NMP03_RS15320

100.0%

NZ_CP101740.1:

Lassopeptides: NMP03_RS15320_lassopeptide

1

GSTMGFSDGETGLMWHPGLYDD

22

GSTMGFSDGETGLMWHPGLYDD

1

GSTMGFSDGETGLMWHPGLYDD

22

77.3%

NZ_CP098774.1:

Lassopeptides: NDN01_RS20480_lassopeptide

1

GSTMGFSDGETGLMWHP

17

GSTMGFSDGETGLMWHP

1

GSTMGFSDGETGLMWHP

17

50.0%

NZ_CP098774.1:

Lassopeptides: allorf_4456163_4456282_lassopeptide

1

GSTMGFSDGETGLMWHPG

18

GST+GF+D ETGL G

1

GSTIGFADVETGLQPKVG

18

with 2 more sequence matches

40.9%

NZ_CP101740.1:

Lassopeptides: NMP03_RS15335_lassopeptide

1

GSTMGFSDGETGLMWHPG

18

GS++GF+D E GL + G

1

GSSIGFADTEVGLQPNAG

18

36.4%

NZ_CP076556.1:

Lassopeptides: GTV57_RS00510_lassopeptide

1

GSTMGFSDGETGLMWHPG

18

GS ++DG GL PG

1

GSGNRYNDGIGGLQNQPG

18

Lassopeptide(s) for NMP03_RS15335

NMP03_RS15335

Leader:

MKYEHENTIELGVVSADTK

Full core:

GSSIGFADTEVGLQPNAG

Putative macrolactam:

GSSIGFAD

NMP03_RS15335

Cleaved off residues:

LSDD

antiSMASH-DB 4.0 matches for NMP03_RS15335

100.0%

NZ_CP101740.1:

Lassopeptides: NMP03_RS15335_lassopeptide

1

GSSIGFADTEVGLQPNAG

18

GSSIGFADTEVGLQPNAG

1

GSSIGFADTEVGLQPNAG

18

72.2%

NZ_CP098774.1:

Lassopeptides: allorf_4456163_4456282_lassopeptide

1

GSSIGFADTEVGLQPNAG

18

GS+IGFAD E GLQP G

1

GSTIGFADVETGLQPKVG

18

44.4%

NZ_CP098774.1:

Lassopeptides: NDN01_RS20480_lassopeptide

1

GSSIGFADTEVGLQ

14

GS++GF+D E GL

1

GSTMGFSDGETGLM

14

with 2 more sequence matches

40.9%

NZ_CP101740.1:

Lassopeptides: NMP03_RS15320_lassopeptide

1

GSSIGFADTEVGLQPNAG

18

GS++GF+D E GL + G

1

GSTMGFSDGETGLMWHPG

18

38.9%

NZ_CP042239.1:

Lassopeptides: FPZ54_RS13295_lassopeptide

11

VGLQPNAG

18

VGLQP AG

10

VGLQPKAG

17

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.61 out of 33.14

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.73 out of 36.53

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.92 out of 25.88

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.08 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.83 out of 29.25

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.78 out of 32.93

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.27 out of 29.25

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

Lasso peptides

RREFinder

TFBS Finder

Azole RiPPs

Core peptide predictions

NMP03_RS15320 - Class II: Cleavage pHMM score: 8.40; RODEO score: 18; Monoisotopic mass: 2354.0 Da; Molecular weight: 2355.5 Da; Number of bridges: 0
NMP03_RS15335 - Class II: Cleavage pHMM score: 6.70; RODEO score: 21; Monoisotopic mass: 1700.8 Da; Molecular weight: 1701.8 Da; Number of bridges: 0

RRE predictions

: RRE type: Lasso_Fused_RRE; Location: 0 - 61; Score: 38.0

TFBS definitions

Regulator	Description

Core peptide predictions

No core peptides found.

NZ_CP101740.1 - Region 8 - hserlactone

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Location: 3,279,013 - 3,299,618 nt. (total: 20,606 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001217.5	0.55	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0000802.5	0.41	saccharide	succinoglycan	Sinorhizobium meliloti
BGC0001786.3	0.35	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000577.3	0.34	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001603.3	0.33	saccharide	gentamicin	Micromonospora echinospora
BGC0000682.3	0.33	terpene	terretonin	Aspergillus terreus NIH2624
BGC0000714.5	0.32	saccharide	sisomicin	Micromonospora inyonensis
BGC0001926.3	0.32	PKS	chrodrimanin B	Talaromyces verruculosus
BGC0000573.3	0.32	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0000696.5	0.31	saccharide	gentamicin	Micromonospora echinospora

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001217.5	0.55	other (other)	PreQ0 Base	Streptomyces qinglanensis
BGC0000802.5	0.41	saccharide	succinoglycan	Sinorhizobium meliloti
BGC0001786.3	0.35	ribosomal	subterisin	Novosphingobium subterraneum
BGC0000577.3	0.34	ribosomal	sphingonodin II	Sphingobium japonicum UT26S
BGC0001603.3	0.33	saccharide	gentamicin	Micromonospora echinospora
BGC0000682.3	0.33	terpene	terretonin	Aspergillus terreus NIH2624
BGC0000714.5	0.32	saccharide	sisomicin	Micromonospora inyonensis
BGC0001926.3	0.32	PKS	chrodrimanin B	Talaromyces verruculosus
BGC0000573.3	0.32	ribosomal	caulonodin I, caulonodin II	Caulobacter sp. K31
BGC0000696.5	0.31	saccharide	gentamicin	Micromonospora echinospora

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

Additional weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.81 out of 20.01

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.09 out of 30.22

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.54 out of 20.97

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.52 out of 24.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
MexT	Global virulence regulator