LOCUS       NZ_AP026074            21053 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 plasmid SNP1.
ACCESSION   NZ_AP026074
VERSION     NZ_AP026074.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1073703)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 706792
            Orig. end                         :: 727845
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026074.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21053
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="9"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21053
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21053
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11053](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11053
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    21..36
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.45"
                     /tool="antismash"
     gene            complement(<249..349)
                     /locus_tag="HEK616_RS42090"
                     /pseudo=""
     CDS             complement(<249..349)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057789.1"
                     /locus_tag="HEK616_RS42090"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGVHGHDPRYFAAVAEELNGTRAAERLSSPSPR"
     gene            458..>610
                     /locus_tag="HEK616_RS39865"
                     /old_locus_tag="HEK616_81150"
                     /pseudo=""
     CDS             458..>610
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057790.1"
                     /locus_tag="HEK616_RS39865"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81150"
                     /product="short-chain dehydrogenase/reductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTENGKVWLVTGARSGFGRVIAEAAVAAGDTVVGTARRTEAQADP
                     AAAHPD"
     misc_feature    727..745
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            728..2143
                     /locus_tag="HEK616_RS39870"
                     /old_locus_tag="HEK616_81160"
     CDS             728..2143
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358509.1"
                     /locus_tag="HEK616_RS39870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81160"
                     /product="MFS transporter"
                     /protein_id="WP_410011389.1"
                     /transl_table=11
                     /translation="MKSLLNSSSASGAAADTPQAARGGSTVVLGAALLGFFLISLDALI
                     VTVALPAIGHSLGGGMSGLQWVVDGYTLIFAALMLSAGSLSDRIGARQAYGGGLVLFAL
                     ASAACGFAPNLGVLVVARLVQGGAAAVMMPASLALVGQGFPDQAKRARAIALWTVGGAV
                     AVAAGPVLGGALTASVGWRWIFFVNLPAGLLALALLARVPASPRLPARLDVVGQVTAVV
                     AMGALTYGVIEGGDKGFGRPLVVVSLLVAAAAAAAFLTAQARGAHPMLPLPLFRSRVVA
                     VSLVVGFMLNAAYYGGVFIFSLYLQQERGQSALHAGLMFIPMTALVAVVNLASAKLAGL
                     FGPRVPMVAGQLLGTAGLLALLTVGAHTNVWVVAALMVPVGLGGALTVPALTAMLLDAV
                     PADRAGTAAAVLNTGRQVGGAIAVAVFGALLAGSETFLAGMRWSMLIAAAGLVLTAGAT
                     FTLPRDGRRGEEA"
     misc_feature    complement(2167..2182)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            2505..3476
                     /locus_tag="HEK616_RS39875"
                     /old_locus_tag="HEK616_81170"
     CDS             2505..3476
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146354.1"
                     /locus_tag="HEK616_RS39875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81170"
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_261958134.1"
                     /transl_table=11
                     /translation="MDQRRIVFVIFEGFQPLDLFGPYEVFEHAHQHTGRYSCQVTAPRP
                     GPVRSGSGLPVDVPYGVAELDPAGIDTLVVAGGGGVDQARHDRALTGWITDAGAGARRV
                     ASVCSGVFLLAAAGLLQGRRVTTHWAREQQLIREHPELVVDCDPIFFRDGRVWTSAGVT
                     AGMDLALALVEDDVGREVAHAIAQEMVLFLRRPGNQSQFSVPLWSGQPSTDPIRAVVSA
                     IHADPGARHGIADLASHAGLSPRHLQRRFTAELGIPPATYVEGVRIEAAKRALAESGHP
                     VETIARRYGFGTAETLRRAFHRHLGIAPSDYRDRFRSTREYL"
     misc_feature    2530..2539
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.29"
                     /tool="antismash"
     gene            3473..4078
                     /locus_tag="HEK616_RS39880"
                     /old_locus_tag="HEK616_81180"
     CDS             3473..4078
                     /EC_number="4.2.1.-"
                     /GO_function="GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627740.1"
                     /locus_tag="HEK616_RS39880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81180"
                     /product="DJ-1/PfpI family protein"
                     /protein_id="WP_261958135.1"
                     /transl_table=11
                     /translation="MTTYGLLTFDGAEELDFAGPWEVFTASSMLRDHADTALLIAERPG
                     AVRCNKGMRVLPDHTLDDHPPLDVLLVPGGNGTRREVSNPVLIDWIRKTAAGTAWTAGV
                     CTGALLLHEAGPARGRRVATHYLFEDTLQARGDITVVRDARYVVDGNLITSQGVSAGID
                     MALWLIGRLHGRDHARAVRRYIQYEPAPPYLADEPSAG"
     gene            4350..4559
                     /locus_tag="HEK616_RS39885"
                     /old_locus_tag="HEK616_81190"
     CDS             4350..4559
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1265: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670436.1"
                     /locus_tag="HEK616_RS39885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81190"
                     /product="ferredoxin"
                     /protein_id="WP_261958136.1"
                     /transl_table=11
                     /translation="MRTVVDLTRCQGYAQCVFLAPEVFQLHGEEGLLYAPAVPDDQIER
                     VRQAAVACPVQAILLGEEVSAGAR"
     gene            4549..5916
                     /locus_tag="HEK616_RS39890"
                     /old_locus_tag="HEK616_81200"
     CDS             4549..5916
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982021.1"
                     /locus_tag="HEK616_RS39890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81200"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_261958137.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 9.6e-45, bitscore:
                     148.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGDLADGRVVIVGASLAGLRAAETLRTEGFTGSLTVVGDEPHPP
                     YDRPPLSKQVLLGKAAADTTGLPMRRDPDADWRLGTPATGLDLLGKRVVLDDGESLPYD
                     RVLIATGTRARPWPNPEEAALDGVFTLRTRDEAGRLAECLAAGPQRVLVIGAGFTGSEI
                     ASACRERGLAVTVAERGPAPLVGALGGTLSRLAAVMQRNHGVDLRCGVTVTALRGDGNG
                     RFTGADLSDGTRVDADLCVVALGAVRNVEWLAESGLAAGPRGIACDAGCRAFTMYGIVT
                     DDVFVAGDVSRFPHPLFGYQMLSLEHWGNAVEQAEVAAHNMVNPGPLQRPHLAIPTFWS
                     TQFGFNIKSVGVPTYSDHVVIAQGSLEARRLAMVYGYQGRVTAAVTVDMAKSLDYYRSL
                     IETAAPFPPPTGAADRAIAADIPIPSDVPDPKGLSHGPTVALTGYLPDRRLTVVRP"
     gene            5954..7138
                     /locus_tag="HEK616_RS39895"
                     /old_locus_tag="HEK616_81210"
     CDS             5954..7138
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670438.1"
                     /locus_tag="HEK616_RS39895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81210"
                     /product="cytochrome P450"
                     /protein_id="WP_261958138.1"
                     /sec_met_domain="p450 (E-value: 3.8e-28, bitscore: 94.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEPDTLLARITDYTSRPYPYPLYAELREAGPVVRQADGSCLVGTY
                     HEIAALLHDPRMSVDPRTRGEVTHKPPFLRLDDPEHHRLRTLAMRPFGPPHSPGRVDAM
                     RGEIDRITEGLMEPFEAGSQVDIVDDFAYPLPVTVICRLLGVPREDEPLFRAWSDALVA
                     AADVRPEADTTEADKAGDQARIEMGGYLVNLAEQRRGRPGDDMLSAFVNEPDPALRLTQ
                     EELAETAVLLLIAGHETTVNLITNGVLTLLRQPEHLDHLRREPALLPRAVEELLRYEPP
                     VHMRERVPHADIEVSGTTISPGTSVVLVLASGNRDPKRFPDPDRFDPTRPDIEHLGFGG
                     GIHLCYGAPLARIEAESALGALIPWLGSARLSQDPPPYRQNAMLRGPRHLPVDL"
     misc_feature    7648..7663
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.69"
                     /tool="antismash"
     gene            7669..8169
                     /locus_tag="HEK616_RS39900"
                     /old_locus_tag="HEK616_81220"
     CDS             7669..8169
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015824.1"
                     /locus_tag="HEK616_RS39900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81220"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_261958139.1"
                     /transl_table=11
                     /translation="MAELVQERGAELHRYGFLLCGDRMEAADLVQEALVRVFSRPRRAW
                     ELGEAEAYVRRTMLNRYLDLHRRRARWIRALPRLVSTAPVADHSTDVMHRIDLMDAMRG
                     LSPRQRACVVLRYYEDLSTADIAARLGCGEGTVKRHLSDALARLGARLRTRAHHVAEER
                     LET"
     gene            8166..8900
                     /locus_tag="HEK616_RS39905"
                     /old_locus_tag="HEK616_81230"
     CDS             8166..8900
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS39905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEK616_81230"
                     /product="hypothetical protein"
                     /protein_id="WP_261958140.1"
                     /transl_table=11
                     /translation="MTPEELREQLLAAAHRTPPPEIDTDRVRSRVRRRRSVRYTALAGA
                     GLAVTAAAVLNTLPSTSPSETRTPTGSVHRQPTATGGTPPGGNQTGVPPLPHYTCGTRI
                     PASSGRGTVTVRISGVRKAPDGAPRVSYAATATAPSELSGAPRILVLKEGRVVAGQDPA
                     GPPANRPTGEENTARRTTVAPDRPQHVRLAPVPGRPCAGTTWSAMWKGGYEVAVVLATQ
                     RAAPPGTWAPDALVVARAPLVG"
     gene            8947..9573
                     /locus_tag="HEK616_RS39910"
                     /old_locus_tag="HEK616_81240"
     CDS             8947..9573
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS39910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEK616_81240"
                     /product="hypothetical protein"
                     /protein_id="WP_261958141.1"
                     /transl_table=11
                     /translation="MRRTLLPAVPVLLLVLAGCGSRSDAVTVDPAHPDSTTTSPSLREQ
                     DRSLPCGRTVDRPVVVSGPDEPLQLTVTSLRRQGSGLRTAYRITSGDRKEMLSLPIGEV
                     PPTARLLKHGVVVGRQKPAADKGPDGSPAIGYPVGRRPYTGTLAIDSLCDGTTWSDVDR
                     HPGDYQVAVVMSVQPHGEPSDSRPAGGVLPLPLLEATRDLSAKGR"
     gene            9730..10008
                     /locus_tag="HEK616_RS39915"
                     /old_locus_tag="HEK616_81250"
     CDS             9730..10008
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971253.1"
                     /locus_tag="HEK616_RS39915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81250"
                     /product="polyprenyl synthetase"
                     /protein_id="WP_261958142.1"
                     /transl_table=11
                     /translation="MTTEAGRREGLDEQAVLLVAGLADLALSTVGSVVGAVRGLLRRSD
                     AAQLAAEAEQDLRARGRLVVDRYTAVPPAHLEVLAQHVLARQARDGA"
     gene            10001..11053
                     /locus_tag="HEK616_RS39920"
                     /old_locus_tag="HEK616_81260"
     CDS             10001..11053
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240939.1"
                     /locus_tag="HEK616_RS39920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81260"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_261958143.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 5.7e-35, bitscore:
                     116.6, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEQWDASAFKARVDAVLREVVSEEADQLTAIDPALGPVAGQLES
                     AVAQGKRLRAAFCYWGWRASGQPDSDAMVRAAASMELVHAAAIVHDDLIDDSPLRHGRP
                     TAHLALRSTVRRRPRAAHAARSLAMLVGDALMSLAGQLFATSGLPAAYLSRARPLWGVL
                     ARELIAGECLEILHTAGAPDTVASLKVIRYKTAKYTVEQPLLIGGALAGAGERMRAGYR
                     AYGVPLGEAFQLRDDLLGLFGDPHVTGKANIDDLRGYRPTALLAETWRLADETERELLR
                     GLLGRSDPDGRCLQAARELMFRLKAPDRVEAMISQRVDEALAAVDDLRLPPPAAKALQT
                     LAHSATHREY"
     gene            11091..12254
                     /locus_tag="HEK616_RS39925"
                     /old_locus_tag="HEK616_81270"
     CDS             11091..12254
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019066651.1"
                     /locus_tag="HEK616_RS39925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81270"
                     /product="oxygenase MpaB family protein"
                     /protein_id="WP_261958144.1"
                     /transl_table=11
                     /translation="MTYTEASLDALRQVGDELADATVAALFERGEVGTFNTLMRYVSTA
                     GAPLPDGLPDVAREYLQATSVPPAWVDWGEMEKARLFFLDNNVHISTALSFASMPACYV
                     VPHVAKLLSATHSLKYPAKRMAETGQFTVYLMQPGAFEAGSRFIPAAQKVRLLHASIRH
                     HLQREDRWDIASLGVPICLEDMIGGQMFFSLLVLDSLHRLGIHMSEEGAQAYFYAWRVV
                     GAMLGVDQNAVPKTLQEARQFLDLYMVRHMGPSEEGAHLTRQLIDLYEEVVPGTFFDPI
                     VSALIRYLIGDTCADWLKVPRTPWDTVVKAVPHLLGVLETVEDRSPFGAWALDRLGHLT
                     TIFELSSLTRGRVMHYAIPEHLKKDYGISDTVPASRRWTPPAATLTP"
     gene            complement(<12354..>12524)
                     /locus_tag="HEK616_RS39930"
                     /pseudo=""
     CDS             complement(<12354..12524)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037627.5"
                     /locus_tag="HEK616_RS39930"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPPAEDHAGGHRGERGQGRFAELCATGAVATHREDHRTIDHPSVG
                     PITVDCDVLTDG"
     gene            complement(12511..12711)
                     /locus_tag="HEK616_RS39935"
     CDS             complement(12511..12711)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS39935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261958145.1"
                     /transl_table=11
                     /translation="MTSANAGLDRRSLLRLGIGGLAAVLAGCAAESGPVEQLRFATGRR
                     AGRTTPSVRHWPKASPRAATG"
     gene            12938..14167
                     /locus_tag="HEK616_RS39940"
                     /old_locus_tag="HEK616_81290"
     CDS             12938..14167
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018851955.1"
                     /locus_tag="HEK616_RS39940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81290"
                     /product="PP2C family protein-serine/threonine phosphatase"
                     /protein_id="WP_261958441.1"
                     /transl_table=11
                     /translation="MDYAALFAATPSPYLVLGPDLVIVEVNQAYLDATVRTREDLIGKY
                     VFDAFPDNPADPQADGVRNLSASLHRVLTTGEPDPMALQKYDIPIMGRPGVFEERWWSP
                     INTPILAPDGTVVWIIHRVEDVTAFVQARTAGARPPTGQLTEREALEAELYARARELQR
                     LNEELRQAHGRERQVAVTLQEGMLQSPDLARHQNVAVRYLPAAGSLNVCGDWYDVADLT
                     DGRFSVAVGDVVGHGLEAAAVMGMLRSALSAAIRALERPAQALEVLGLYARSVEGALNT
                     TAVKALVDPASCLIIYSNAGHPPPILVHGDGTCELLDRATDPPLGVRPLHVPRPQATAI
                     YSPGDTLVLYTDGLIERRDEDIDTGLARLTDTLSQSHQLAPEHLADTLLARLGLAGGGR
                     DDVALIIVRL"
     gene            complement(14233..14751)
                     /locus_tag="HEK616_RS39945"
                     /old_locus_tag="HEK616_81300"
     CDS             complement(14233..14751)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741238.1"
                     /locus_tag="HEK616_RS39945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81300"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_261958146.1"
                     /transl_table=11
                     /translation="MTQSTPEAGNGPAEIGQEATVPVPAAASGGPVSHAIFRLARLHRM
                     FAGQLLRRIGLHPGQELVMMHLWELGPQRQTDLVRLMDSDAATMTRTVRRLEQAGFVRR
                     RPSPTDKRASLIEPTAASHALRDQVEQVWSQLEDLSTAGLTDDERTAALATLERLEHNL
                     VQAATHPQS"
     misc_feature    14820..14833
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    14855..14870
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.55"
                     /tool="antismash"
     gene            15002..16072
                     /locus_tag="HEK616_RS39950"
                     /old_locus_tag="HEK616_81310"
     CDS             15002..16072
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387028.1"
                     /locus_tag="HEK616_RS39950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81310"
                     /product="alkene reductase"
                     /protein_id="WP_261958147.1"
                     /transl_table=11
                     /translation="MTTAFDPIVLGGKPLANRVVMAPMTRSRAYGPGAEPTELMATYYA
                     QRASAGLIVTEGIQPSPVGQGYPDTPGLHTPGQVRAWRTVTDAVHREGGVIFAQLMHTG
                     RIGHPSLLPDGLVPVGPSPVAAKGQVYTHEGPKEFVTPKELSEAEIRQTIADFAAAARN
                     AVEAGFDGVEIHGANGYLIHQFLAPNSNQRTDAWGGTTEGRIRFAVEVVTAVAEAIGSD
                     RVGLRISPGNVYNDMAEDDPAEVYGALLERLAGLELAYLHLMEGPDRSLTARLRKDWPG
                     TFVLNPFTHPDVTGPDALQLIEDGSADMVAYGALFLANPDLPRRLATGGPFNTPDKATF
                     YGGDHRGYTDYPTHTA"
     gene            16156..17061
                     /locus_tag="HEK616_RS39955"
                     /old_locus_tag="HEK616_81320"
     CDS             16156..17061
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (14-299). E-value: 4.8e-30.
                     Score: 96.7. Matches aSDomain:
                     nrpspksdomains_HEK616_RS39955_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605535.1"
                     /locus_tag="HEK616_RS39955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81320"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_261958148.1"
                     /sec_met_domain="ADH_N (E-value: 5.1e-08, bitscore: 28.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.5e-11, bitscore:
                     39.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQAITFSAYGPPEVLQLSEVTLPEPGPGQVRIRVRAASVNPLDI
                     KIRSGLMAGAVPARFPVIPGLDAAGVVDAVGEGADASVGDEVLGGTVGGSYGEHALLDR
                     PVAKPAALSWEVAASLVTVGRTAFRVLGQLGAQAGQTLLIHGAGGSVGTIAVQLAVARG
                     ITVVGTAGEHDIERVTALGATAVRYGDGWVERVTAAAAQGVDFVFDASGAGVLADSVAL
                     TGDSAHVITIADMSAAQHGVAFSAGSADQAEESLPELVQLAAGGHLDVPVWRAYPLDEA
                     ATAHADLEARRNQGKVVLLP"
     aSDomain        16198..17052
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS39955_PKS_ER.1"
                     /evalue="4.80E-30"
                     /label="HEK616_RS39955_PKS_ER.1"
                     /locus_tag="HEK616_RS39955"
                     /protein_end="299"
                     /protein_start="14"
                     /score="96.7"
                     /tool="antismash"
                     /translation="VLQLSEVTLPEPGPGQVRIRVRAASVNPLDIKIRSGLMAGAVPAR
                     FPVIPGLDAAGVVDAVGEGADASVGDEVLGGTVGGSYGEHALLDRPVAKPAALSWEVAA
                     SLVTVGRTAFRVLGQLGAQAGQTLLIHGAGGSVGTIAVQLAVARGITVVGTAGEHDIER
                     VTALGATAVRYGDGWVERVTAAAAQGVDFVFDASGAGVLADSVALTGDSAHVITIADMS
                     AAQHGVAFSAGSADQAEESLPELVQLAAGGHLDVPVWRAYPLDEAATAHADLEARRNQG
                     KVVL"
     CDS_motif       16348..16383
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS39955_0001"
                     /evalue="7.80E-03"
                     /label="PKSI-ER_m2"
                     /locus_tag="HEK616_RS39955"
                     /protein_end="76"
                     /protein_start="64"
                     /score="9.4"
                     /tool="antismash"
                     /translation="GLDAAGVVDAVG"
     CDS_motif       16570..16632
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS39955_0002"
                     /evalue="3.10E-06"
                     /label="PKSI-ER_m5"
                     /locus_tag="HEK616_RS39955"
                     /protein_end="159"
                     /protein_start="138"
                     /score="19.7"
                     /tool="antismash"
                     /translation="GQTLLIHGAGGSVGTIAVQLA"
     misc_feature    17253..17267
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            17271..17516
                     /locus_tag="HEK616_RS39960"
                     /old_locus_tag="HEK616_81330"
     CDS             17271..17516
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013777.5"
                     /locus_tag="HEK616_RS39960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81330"
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="WP_261958149.1"
                     /transl_table=11
                     /translation="MERHHPPHSGPRPARYGELHTAIGSISTKVLADRLRELVEAGLVT
                     HDRLPPGPATYTLTAEGRALLPALEQIRSWAHHRTT"
     misc_feature    complement(17546..17560)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.95"
                     /tool="antismash"
     gene            17562..18356
                     /locus_tag="HEK616_RS39965"
                     /old_locus_tag="HEK616_81340"
     CDS             17562..18356
                     /NRPS_PKS="Domain: Abhydrolase_1 (23-248). E-value:
                     2.4e-24. Score: 78.2. Matches aSDomain:
                     nrpspksdomains_HEK616_RS39965_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740968.1"
                     /locus_tag="HEK616_RS39965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81340"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_261958150.1"
                     /sec_met_domain="Thioesterase (E-value: 2.8e-07, bitscore:
                     26.8, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.6e-17, bitscore:
                     60.7, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 1.2e-18, bitscore: 63.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSARALPEATLARTVLGSGPGLALAHGAGSSVAGTYGPILEGLAA
                     HHTVVGVDYPGSGDTPRSATPLSLDDLADQLVAAAVAEGLDTFAVSGYSLGGPVAIRAA
                     ARHPERVTALVLTATFPHRDNRLALALPIWRKLAEPGNRALLAEFLTMMALGADALEAM
                     PNEQLQQALAFTAASVADGTPEHAELAPRVDVREDLPGIQAPTLVISTTQDWFTSTRLH
                     RQLADAIPGAQLVEIRTGHLPMVERPEEWQKLITEFLDQHSA"
     aSDomain        17631..18305
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS39965_Abhydrolase_1.1"
                     /evalue="2.40E-24"
                     /label="HEK616_RS39965_Abhydrolase_1.1"
                     /locus_tag="HEK616_RS39965"
                     /protein_end="248"
                     /protein_start="23"
                     /score="78.2"
                     /tool="antismash"
                     /translation="LAHGAGSSVAGTYGPILEGLAAHHTVVGVDYPGSGDTPRSATPLS
                     LDDLADQLVAAAVAEGLDTFAVSGYSLGGPVAIRAAARHPERVTALVLTATFPHRDNRL
                     ALALPIWRKLAEPGNRALLAEFLTMMALGADALEAMPNEQLQQALAFTAASVADGTPEH
                     AELAPRVDVREDLPGIQAPTLVISTTQDWFTSTRLHRQLADAIPGAQLVEIRTGHLPMV
                     ERP"
     CDS_motif       17832..17879
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS39965_0001"
                     /evalue="1.80E-03"
                     /label="NRPS-te1"
                     /locus_tag="HEK616_RS39965"
                     /protein_end="106"
                     /protein_start="90"
                     /score="10.9"
                     /tool="antismash"
                     /translation="SGYSLGGPVAIRAAAR"
     gene            complement(18523..19290)
                     /locus_tag="HEK616_RS39970"
                     /old_locus_tag="HEK616_81350"
     CDS             complement(18523..19290)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018089363.1"
                     /locus_tag="HEK616_RS39970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81350"
                     /product="carbonic anhydrase"
                     /protein_id="WP_261958151.1"
                     /transl_table=11
                     /translation="MDRAGHPDRRALLGIGLAGAAAALAGCSSTRTAAPTSASPEARAA
                     QTTTASPQARPTTPAAAFARLMEGNKRWVSGDLHHPDRDPNRRQLVAQHQEPFGSILSC
                     IDSRVPPELLFDTGLGDLYVMRTGGEAIDPVVTGSVEYGPMTSGTPLVVVLGHQRCGAV
                     AAAYKSLRDGKPLPGNLQAIVKALEPAYRQAVRQGGADPVETMARAQVTVTAADLRSNQ
                     DLGPLVAKGTLAVVGAYYSLDTGKVEVLSGAPS"
     gene            complement(19603..20169)
                     /locus_tag="HEK616_RS39975"
                     /old_locus_tag="HEK616_81360"
     CDS             complement(19603..20169)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602219.1"
                     /locus_tag="HEK616_RS39975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_81360"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_261958152.1"
                     /transl_table=11
                     /translation="MSKPPGTRQSIIDAVLRIIGEDGVAAVTNRRIAKEANVSLGSVTY
                     HFATQHEMLRESLLHFVAEETRRLTELAAQCQTDGMDADRAAATVSQVAGGTPFDSRHI
                     APFELYIQAGRDERLRSAAAESFAAYDQLAARILTGLGVPDAERLASSAIALVFGLQLR
                     SLATGQPAEELVDAFMLLARGAQHS"
     misc_feature    complement(20163..20177)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.1"
                     /tool="antismash"
     misc_feature    20349..20367
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.93"
                     /tool="antismash"
     misc_feature    20771..20780
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 acttgagcct tatttggccc attgaagccc attctcttcg caacgagagc cgcgagggcg
       61 aggaggagaa cggagaagaa ccactgcagg atgcgctggg acttccttcg gagtgtcact
      121 gtgtcctctt gccgcttcat tccggtctct ccttcttctc tgcccgctcc gtcagggtgg
      181 ccgtcccatg gtcggccacc ctgacgacta ggtgatcggc acgttttcgg gatctgcggc
      241 caggctaaga gcgcgggctg ggagacgaca gcctctcggc ggcccgtgtg ccgttcaact
      301 cctcggctac tgcggcgaag tagcgcggat cgtgtccatg gacacccata acgcatagct
      361 atcacgactg gtcttggacg tgaccccgga tcccggcgaa ggatggtggc tgtcgacggg
      421 cgggtcccga acggcagttg accagggaga cgaagagatg accgagaacg gcaaggtctg
      481 gctggtcacc ggcgcgcgca gcggattcgg gcgagtcatt gccgaggctg cggtcgccgc
      541 cggtgacacg gtggttggca cggcccgacg gaccgaggcg caggccgacc cggccgccgc
      601 acatcccgat ccgcttactt gaccgcgccg gggtgctccc gctcccagcc ggccagtcat
      661 tggtcgttcc tgacctccac ctcccctctc gattgcacgc attcaagacc gtttcaggag
      721 tgccctcatg aaatcgctac tcaattcctc ctctgcctcc ggcgccgcgg ccgatacgcc
      781 gcaggcggcc cggggtggtt ccaccgtggt gctcggtgcc gccctgttgg gcttcttcct
      841 catctcgctg gacgcgttga tcgtgaccgt cgcactgccc gccatcggcc acagcctcgg
      901 cggcggcatg tccggcctgc agtgggtggt ggacggatac acgctgatat tcgccgccct
      961 gatgctctcc gcgggttccc tctccgaccg catcggggcg cgccaggcgt acggcggcgg
     1021 cctggtgctt ttcgcgctgg cctcggcggc gtgcgggttc gcgcccaacc tcggtgtgct
     1081 ggtggtggcg cggctggtgc aggggggtgc ggcggcggtg atgatgccgg cgtccctcgc
     1141 cctggtcggg cagggctttc ccgaccaggc gaagcgggct cgggccatcg ccctctggac
     1201 cgtgggcggc gcggtcgcgg tggcggccgg tccggtgctc ggcggggccc tcaccgcttc
     1261 cgtcggctgg cggtggatct tcttcgtcaa cctcccggcg ggcctgctgg cgctcgctct
     1321 gctggcccgg gtgccggcct ccccgcggct gcccgcacgg ctggacgtgg tggggcaggt
     1381 gacggcggtg gtcgcgatgg gcgcgctgac gtacggcgtg atcgagggcg gcgacaaggg
     1441 cttcggccgg ccgctcgtgg tggtgtcgct gctggtggct gccgccgcgg cagccgcctt
     1501 cctcaccgcg caggccaggg gcgcgcatcc gatgctcccg ctgccgctgt tccgttcgcg
     1561 ggtggtggcg gtgtccttgg tggtcggctt catgctcaat gccgcctact acggaggggt
     1621 gttcatcttc agcctgtatc tgcagcagga gcgcgggcag tccgccctgc acgcgggcct
     1681 gatgttcatt ccgatgactg cactggtcgc cgtggtgaat ctggcctcgg ccaagctggc
     1741 cgggctgttc ggcccgcggg tgccgatggt cgcaggacag ctgctcggga cggccggcct
     1801 gctggcactg ctcaccgtcg gcgcgcacac caacgtgtgg gtggtggcgg cgctgatggt
     1861 gcccgtcggc cttggcgggg cgctgacggt gccggcgctg accgcgatgc tgctcgacgc
     1921 cgtgcccgcg gaccgggcgg gtaccgcggc cgcggtgctc aacaccggcc gccaggtggg
     1981 cggagcgatc gcggtggcgg tcttcggggc gctgctggcg gggtcggaga ccttcctggc
     2041 cggtatgcgg tggagcatgc tgatcgcggc ggccgggctc gtactgacgg ccggcgcgac
     2101 gttcacgctg ccgcgggacg ggcgtagggg agaggaagcg tgaggttggt tgcgagcctg
     2161 ggggccggga cgcgggggtg actggtgctg ggtgtcgcgt gacggcgcag cgggagccgg
     2221 cggacaaccc aacaccaatt ccccttccca ttcccattcc ccactccaac tccacgaacc
     2281 ccgctccgtg aacagccgta cggcatcgga tagttgccac gcgctacggg caactcgccg
     2341 tccgcgggga gtgccctcgc ccttcgtggc cacccggtgg cggtcgtgtc ggcgggccgg
     2401 accgtgccaa gccgtcccac tgctgctgtc acacccctgg tatggcgcaa agcgtggggt
     2461 atctgtcgtt gacgccatcc gatgccggat cgcagggtgg agaggtggat cagcgccgca
     2521 tcgtcttcgt gattttcgag gggttccaac cccttgacct cttcggtccg tacgaggtgt
     2581 tcgagcatgc ccatcaacac accggcaggt actcctgcca ggtcacggca ccgcgaccgg
     2641 ggccggtacg gtccggcagc ggccttccgg tagacgttcc gtacggtgtg gccgagctgg
     2701 accctgcggg gatcgacacg ctggtggtcg ccggtggcgg aggagtcgac caggcccgtc
     2761 acgatcgggc gctgacaggc tggatcaccg acgcgggggc cggcgcccga cgcgttgcct
     2821 cggtgtgtag cggggtgttc ctgctggccg ccgccggact gctccaggga cggagggtga
     2881 ccacccactg ggcacgtgag cagcaactca tacgggagca tcccgaactg gtcgtcgact
     2941 gcgatccgat cttcttcagg gatgggcggg tgtggacatc ggccggggtg acggcgggga
     3001 tggacctggc actcgccctg gtggaggacg acgtgggccg ggaggtcgcc cacgccatcg
     3061 cgcaggagat ggtgctgttc ctgcgccgcc caggcaacca gtcgcagttc agcgtgccgt
     3121 tgtggtccgg gcagccgtcc actgatccca tccgcgcggt ggtctcggcg atccacgccg
     3181 accccggtgc ccggcacggc atcgccgacc tcgcctcgca cgccgggctg agcccccgtc
     3241 acctccagcg caggttcacg gccgaactcg gcatcccgcc cgccacgtat gtcgagggcg
     3301 tacgcatcga ggcggcgaaa cgggccctcg ccgagtccgg ccaccccgtc gagaccatcg
     3361 cacgacggta cggcttcggc accgcggaga cgctgcgccg cgcgttccac cggcacctgg
     3421 gcattgcacc gtccgactac cgcgaccgat tccgttccac cagggagtac ctatgaccac
     3481 ctacggactg ctgacgttcg acggtgcgga ggagttggac ttcgccgggc cttgggaggt
     3541 gttcaccgca tcctccatgc tgcgcgacca tgccgacacc gcgctgctca tcgccgaacg
     3601 ccccggcgcc gtccgctgca acaagggcat gcgggtgctg ccggaccaca ccctggacga
     3661 ccaccctccg ctggacgtcc tcctggtccc cggcggcaac gggacccgcc gcgaagtgtc
     3721 caaccccgtg ctcatcgact ggatccgcaa gaccgccgcc ggcaccgcct ggaccgccgg
     3781 ggtgtgcacc ggagccctgc tgctgcacga ggcaggcccg gcccgcggcc gccgggtcgc
     3841 gacccactac ctgttcgagg acaccctgca ggccaggggc gacatcaccg tggtccgcga
     3901 cgcccgttac gtcgtcgatg ggaacctgat caccagccag ggcgtctcgg ccgggatcga
     3961 catggccctg tggctcatcg gccgcctcca cggccgcgac cacgcccgcg ccgtccgccg
     4021 ctacatccag tacgagccgg ctccgcccta cctcgccgac gagccgtccg ccggctgaca
     4081 ccccgtgacg gacgaggccg agggtgtgcc gccggccgga gcgaacggtg ctcaggccac
     4141 cacttccgat acccgaccgg tgaatgcctg aaggcctcgg tgtcaccacc gccacgggca
     4201 tcggtgatga cggccgcgag gtagcccggg tccgacgagc cggtgggccg gcccttcgtc
     4261 accggcgggc gccgctcgct gggtgcgggc gtccggatct cgccccatgc tggaagtgag
     4321 gggggtaggc gacggaggaa gtggtgagga tgcggaccgt tgtggacctc acgcgctgcc
     4381 agggctatgc gcagtgcgtg ttcctcgcgc cggaagtgtt ccagctgcac ggggaggagg
     4441 gattgctgta cgcccctgcc gtccccgacg accagatcga gcgggtgcgc caggccgcgg
     4501 tggcctgccc ggtacaggcg atcctcctcg gtgaggaggt gagcgccggt gcccggtgac
     4561 ctcgcagacg gccgtgtcgt catcgtcggc gcgtcgctgg ccgggttgag ggccgcggag
     4621 acgctgcgta cggagggctt caccggctca ctgaccgtgg tcggcgacga gccccatccg
     4681 ccctacgacc ggccgccgct gtccaagcag gtgctcctcg gcaaggcggc ggcggacacc
     4741 accggactgc cgatgcggcg ggacccggac gccgactggc ggctggggac acctgccacg
     4801 gggctggacc tgctcgggaa acgggtggtg ctggacgacg gcgagtcact gccgtacgac
     4861 cgggtgctca tcgccaccgg gacccgcgcg cggccctggc ccaacccgga ggaggccgcc
     4921 ctggacgggg tgttcacgct ccgtacccgg gacgaagccg gaaggctggc cgagtgtctg
     4981 gccgccgggc cgcagcgggt gctggtgatc ggtgccggtt tcaccggctc ggagatcgcc
     5041 tcggcctgcc gggaacgggg gctggcggtc acggtcgccg aacgcgggcc cgcacccctg
     5101 gtgggcgcgc tgggcggcac cctgtcgagg cttgccgctg tcatgcagcg caaccacggc
     5161 gtcgacctgc gctgcggggt cacggtcacc gccttgcgcg gcgacgggaa cggccggttc
     5221 accggtgcgg acctgtccga cggcacccgc gtcgacgcgg acctgtgcgt cgtggcgctg
     5281 ggagccgtcc gcaacgtcga atggctcgcg gagtccgggc tggccgccgg cccgcggggg
     5341 atcgcctgtg acgccggctg ccgggccttc accatgtacg ggatcgtcac cgacgacgtc
     5401 ttcgtggccg gcgacgtgtc ccgctttccc cacccgctgt tcggatacca gatgctctcc
     5461 ctggagcact ggggcaacgc ggtcgagcag gccgaagtgg cggcccacaa catggtcaat
     5521 ccagggccac tgcagcgccc gcacctggcc atcccgacgt tctggtcgac tcagttcggg
     5581 ttcaacatca agtcggtggg ggtgcccacc tactccgacc atgtggtcat tgcccagggc
     5641 tctctggagg cgcgccgtct ggcgatggtc tacggctacc aagggcgggt caccgccgcg
     5701 gtcaccgtcg acatggccaa gtcgctcgac tactaccgaa gcctgatcga gacggccgct
     5761 ccgttcccgc ccccgaccgg agcggcggac cgtgcgatcg cggccgacat cccgatcccg
     5821 tccgacgtcc cggatccgaa ggggctgtcc cacggcccca ccgtggcact cacgggctat
     5881 ctgcccgatc gccgactgac cgtggtacgg ccctgaggct gagccacgtc ccgctccacc
     5941 acgaggagtc accatggagc ccgacacctt gctggcacgg atcaccgact acaccagtcg
     6001 cccctatccc tacccgctgt acgcggaact gcgcgaggcc ggtcccgtgg tgcggcaggc
     6061 ggacggcagc tgtctggtcg gcacctacca cgagatcgca gctctgctcc acgacccgcg
     6121 gatgagtgtc gatccccgta cccgtggcga ggtgacacac aagccgccgt tcctgcggct
     6181 cgacgacccg gagcaccacc ggctgcgcac tttggccatg cggccgttcg gtcccccgca
     6241 cagtccgggc cgggtcgacg ccatgcgcgg cgagatcgac cggatcaccg agggactgat
     6301 ggagccgttc gaggcgggca gccaggtcga catcgtcgac gatttcgcct accccctgcc
     6361 cgtcacggtg atctgccgtc tgctcggcgt gccgcgtgag gacgagccgc tgttccgggc
     6421 ctggtccgac gccctggtcg cagccgccga cgtcaggccc gaagcggaca ccaccgaggc
     6481 ggacaaggcg ggcgaccagg cgcggatcga gatgggcggg tacctggtga acctcgccga
     6541 gcagcgccgt ggccggccgg gcgacgacat gctctccgcc ttcgtcaacg aaccggaccc
     6601 ggccctgcgg ctcacccagg aggaactcgc ggaaaccgcc gtgctgctcc tcatcgccgg
     6661 acacgagacc acggtcaacc tgatcaccaa cggtgtgctc accctgctgc gccaacccga
     6721 gcatctggac cacctgcggc gcgagcccgc cctgctgccg cgagcggtcg aggaactgct
     6781 gcgctatgaa cccccggtcc acatgcggga gcgcgtcccc cacgcggaca tcgaggtcag
     6841 cggtaccacg atctccccgg gcacgtccgt cgtcctggtg ctggcctcgg gcaaccgcga
     6901 ccccaagcga ttccccgacc ccgaccggtt cgaccccacc cgcccggaca tcgagcacct
     6961 cggcttcggc ggcggcatcc acctgtgcta cggcgcaccc ctcgcccgca tcgaggccga
     7021 gtccgcgctc ggcgcgctga tcccgtggct cggctcggca cgcctgtccc aggacccgcc
     7081 cccctaccgg cagaacgcca tgctccgcgg cccccgccac ttgcccgtcg acctctgagg
     7141 ccgccacggg gcggtgcgcc tctgatgcgg gctcagtcgt gggcgcgggc cggccgggat
     7201 gcctgggccg gatccggtcg gtggtgtgtg actgattcct tcggcggact ttggccggac
     7261 tgactttggc cggactcccg acccgcacac gccagttgcc tgcgctgcac gcaagacggt
     7321 cggccgaatc cgggccggcg tgagggcgat tcccgcggat cgagcaccac ccgatacgcg
     7381 cctggacgga gtacggcccg aaccggcccc acgccgtgac ggccgcgagc gtgagcagca
     7441 gtacggtcac gatgatcatc tgcgtctcgc cgcgagttcg ccgacgaccg ccgcagggtg
     7501 ctcgcggcgc ggaggatctc ccggccgatt caccgatcgt ggacggatga tggcccaggt
     7561 cggccgcgct cgaccatctt ctcgcggcaa gcccccaacc ggacgggcga gttgttcgtg
     7621 ataccgggtg aggggatcgg agggaatgtg gccgaatggt cgaggcttgt ggccgagctc
     7681 gtgcaggagc gcggcgcgga gctgcatcgg tacggcttcc tgctgtgcgg cgaccgcatg
     7741 gaggctgccg acctggtgca ggaagcactg gtacgggtct tctcccggcc ccgccgtgcg
     7801 tgggaattgg gggaggcgga ggcgtacgta cgccgcacga tgctcaaccg ctacctggat
     7861 ctgcaccggc gccgtgcccg ttggatccgc gcgcttccac ggctcgtgtc cacggcaccg
     7921 gttgccgacc acagcacgga cgtcatgcac cggatcgacc tcatggacgc catgcgcgga
     7981 ctgtcgccga ggcagcgggc ctgcgtggtg ctccgttact acgaggactt gtccaccgcg
     8041 gacatcgcgg cgcgcctcgg ctgtggtgaa ggcacggtca agcgtcatct gagcgacgcg
     8101 ctggcacggt tgggcgcccg gttgcgaaca cgcgcacatc acgtcgcgga ggaaaggctg
     8161 gagacatgac gccggaggag ctgcgcgaac agttgctggc cgccgcgcac cgcacaccgc
     8221 cgccggagat cgacaccgac cgcgtgcgga gccgggtgcg ccggcgcaga agcgtccggt
     8281 acaccgccct ggccggggca ggcctggccg tgaccgccgc ggcggtgctg aacacgttgc
     8341 cgtccacctc gccatcggag accaggacac cgaccggctc ggtccaccgg cagcccacag
     8401 cgactggcgg cacaccaccg ggcggaaacc agaccggcgt accaccgctc cctcactaca
     8461 cctgcggtac gagaatcccg gcaagttccg gccggggaac cgtgaccgta cgcatctccg
     8521 gtgtgcgcaa ggctcccgac ggcgccccgc gggtcagcta cgcggcgacc gcgaccgcac
     8581 cctccgaact gtccggtgcg ccgcggatcc tcgtcctgaa agaaggccgg gtggtggccg
     8641 gtcaggatcc ggccggacca ccggccaacc gaccgacggg agaggagaac accgcgcgcc
     8701 ggacgaccgt cgcccccgac cgtccccagc acgtccggct cgcacccgta ccgggccgcc
     8761 cctgcgcggg aaccacctgg agtgcgatgt ggaagggcgg ctacgaagtg gccgtcgtcc
     8821 tcgccaccca gcgcgcggcg ccccccggaa cctgggcccc cgatgcgttg gtagtggccc
     8881 gcgcccccct ggtcggatga atgctcggca ccgctctcga cagctcacga cttcacggag
     8941 aaacccatgc gccgcaccct gctgcccgcc gtccccgtcc tgctgctcgt cctcgccgga
     9001 tgcgggagcc ggtccgacgc ggtgaccgtc gatcctgccc atcctgactc caccaccacg
     9061 agcccctcac tgagagagca ggaccgctcg ctgccctgcg gccgcaccgt cgatcgcccc
     9121 gtcgtcgtca gcggccccga cgagcctctg caactgaccg tcacgtccct gcggcgccaa
     9181 ggctctgggc tgcggaccgc ctaccgcatc acctccggcg accggaagga aatgctcagc
     9241 ctgccgatcg gtgaggtccc gcccacggcc cgcctgctca agcacggcgt cgtcgtcggc
     9301 cgccagaagc ccgccgcaga caagggcccg gacggcagcc cggccatcgg gtatcccgtc
     9361 ggtcggcgcc cgtacacggg gacgttggcg atcgacagcc tctgcgacgg caccacgtgg
     9421 tccgacgtgg acaggcaccc cggcgactac caggtcgccg tcgtcatgtc cgtccagccg
     9481 cacggagaac cctccgacag ccggccggca ggcggtgtcc tcccgctgcc cctcctcgaa
     9541 gcaacccgcg acctctccgc gaagggccgg tgaacgcgac agacacccgc cgagcgggaa
     9601 gcgccgcggt cgcggacgca tgtgcgaggt gatccctcgt tggtgagttc tgcgactgcc
     9661 cccgcaaccg ggtggtcacc atgtgagggg gcgcgaccgg catcgcgcgg gggttcatga
     9721 gggggaagcg tgacgactga ggctggacgg cgtgagggtc tggacgaaca ggccgtgttg
     9781 ctggtggcgg gtctggccga tctggcgctg agcacggtcg ggtcggtcgt gggggcggtg
     9841 cgcgggttgc tgcgtcgttc ggatgccgcg cagctggcgg ccgaggcgga gcaggacttg
     9901 agggcacgtg gacgactggt ggtggaccgg tatacggccg tgccgccggc ccatcttgag
     9961 gttctggccc agcacgtcct ggccaggcag gcgcgtgacg gtgcctgagc agtgggatgc
    10021 gtcggccttc aaggcccgtg tggacgccgt gctgcgagag gtcgtcagcg aagaggccga
    10081 tcagttgacg gccatcgatc cggccctcgg cccggtcgcc gggcagttgg agtcggcggt
    10141 cgcgcagggc aagcggctgc gggcggcgtt ctgctactgg ggctggcggg cgtccggcca
    10201 gcccgacagc gacgccatgg tgcgggcggc ggcctccatg gagctggtac acgccgccgc
    10261 gatcgtccac gacgacctca tcgacgacag cccgctacgc cacggccggc ccaccgctca
    10321 cctcgccttg cgctcgacgg tgcgccgccg ccctcgtgcc gcccatgcgg cacgatcact
    10381 ggcgatgctg gtcggcgatg cgttgatgtc gctggcagga cagctcttcg ccaccagtgg
    10441 gctgccggcg gcctatctca gccgggcccg tcccttgtgg ggagtgctgg cccgggagct
    10501 gatcgcgggt gagtgcctgg agatcctgca caccgcaggc gccccggaca cggtggcctc
    10561 gctgaaggtg atccgctaca agaccgccaa gtacaccgtc gaacagcccc ttttgatcgg
    10621 cggtgcgctg gccggggccg gtgagcggat gcgggcgggt taccgcgcct acggcgtgcc
    10681 gctgggggag gccttccaac ttcgcgacga tctgctcggc ctgttcgggg acccgcacgt
    10741 cacgggcaag gcaaacatcg atgacctccg cggttaccgc cccaccgcgc tgctcgccga
    10801 gacatggcgc ctggccgacg agaccgaacg ggaactgctg cggggcctgt tgggccgttc
    10861 ggatcccgac ggtcggtgtc tgcaggcggc ccgggagctg atgttccggc tgaaggcgcc
    10921 ggaccgcgtc gaagccatga tctcccagcg cgtcgacgaa gccctggccg ccgtggacga
    10981 cctgcgcctt cccccgcccg ccgccaaagc cctgcagacc ctggctcact cggccacgca
    11041 tcgcgagtac tgacaaggca aggcggcaca caccaaggga gtcttctgtc atgacctaca
    11101 ccgaggcgtc gctggacgcc ctgcgccagg tcggcgacga actcgcggat gcgaccgtgg
    11161 ccgctctctt cgaacgtggt gaggtgggca cgttcaacac cctgatgcgg tacgtctcca
    11221 ccgcgggcgc gccactgccc gacgggttgc ccgacgtggc ccgcgagtac ctgcaggcaa
    11281 ccagcgttcc gcctgcctgg gtggactggg gggagatgga gaaggcgcgg ctgttcttcc
    11341 tcgacaacaa cgtgcacatc tccacggcgc tgtcgttcgc ctccatgccg gcctgctacg
    11401 tcgtgccgca cgtggcgaag ctgctgtcgg cgacccactc gctgaagtac ccggccaagc
    11461 ggatggcgga gaccgggcag ttcaccgtct acctgatgca gcccggtgct ttcgaagccg
    11521 gcagccgctt catccccgcc gcgcagaagg tccgcctgct gcacgcctcc atccgccacc
    11581 acctgcagcg ggaagaccgc tgggacatcg cgtcactggg tgtgccgatc tgcctggagg
    11641 acatgatcgg cgggcagatg ttcttctcct tgctggtcct cgacagcctg caccgcctgg
    11701 gcatccacat gtcggaggag ggcgcgcagg cctacttcta cgcctggcgg gtggtcggcg
    11761 ccatgctcgg cgtcgaccag aacgccgtgc ccaagaccct tcaggaagca cggcagttcc
    11821 tcgacctgta catggtccgg cacatgggtc cctccgagga gggcgcccac ctgacccggc
    11881 agctgatcga cctgtacgag gaagtcgtcc cgggaacatt cttcgacccg atcgtctccg
    11941 ctctcatccg ctacctcatc ggcgacacct gcgccgactg gctgaaagtc ccgcgcaccc
    12001 cgtgggacac cgtcgtcaaa gcagtcccgc acctgctggg tgtcctggag accgtcgaag
    12061 accgctctcc gttcggtgcc tgggccctgg accgcctcgg ccacctcacc acgatcttcg
    12121 aactgtcctc cctcacccgc ggccgcgtca tgcactacgc catccccgaa cacctgaaga
    12181 aggactacgg catctccgac acggtgcctg cgagccgccg ctggacccca ccggccgcca
    12241 cccttacccc ctgacgcacg aggccacaac gcaggtcggc gcaactcacg ggcgccgccc
    12301 tcgtcctcac ttccgggcac ggtggtcatg acgacgatct tgagctcgga gtcgccgtcg
    12361 gtcaggacgt cgcagtccac cgtgatcggg cccaccgacg ggtggtcgat ggtcctgtgg
    12421 tcctcacggt gcgtggccac cgctccggtt gcgcacagct cggcgaacct cccttgtcca
    12481 cgctcgccgc ggtgcccacc ggcatgatct tcagccggtg gccgctcgtg gcgacgcctt
    12541 cggccagtgc ctgaccgaag gcgtggtacg gcccgccctg cggcccgtgg cgaaccgcag
    12601 ctgctccacg ggaccggact ccgccgcgca cccggccagg acggcggcga gcccgccgat
    12661 gccgagcctc agcagactcc ggcggtcgag cccggcattc gcgctggtca cggcgtgaat
    12721 gtaaccgcgg accagacacg gtggccgtgc ttcgaggcgg tcggcggaaa cgggcggggc
    12781 cggctgcccc gcccgctgtc ccgcaagcgg tcgggcgcga tggagcggcc gacgttgtcg
    12841 cagcaggcgg gtgagcggga gggatcgtag cgtgagagga gcgggcagag ggggtgccgg
    12901 gaggaaggcc ggagaaggga cggtggccgt ggcggggatg gattacgcgg cgctgttcgc
    12961 cgccacgccg agcccgtatc tggtgctggg gccggatctg gtgatcgtgg aggtcaacca
    13021 ggcatacctc gacgcgactg tccggacgag ggaagacctg atcgggaagt acgtcttcga
    13081 tgccttcccg gacaacccgg ccgatcccca ggccgacggg gtgcggaact tgagcgcctc
    13141 gctgcaccgg gtcctgacca cgggcgagcc cgacccgatg gcactgcaga agtacgacat
    13201 ccccatcatg ggccggccgg gggtcttcga ggagcggtgg tggtccccga tcaacacgcc
    13261 gatcctcgcc ccggacggca cggtggtgtg gatcatccac cgggtggagg acgtgacggc
    13321 gttcgtccaa gctcgcaccg ccggggccag gccgccgacc ggccagctga ccgagcgcga
    13381 agcgttggag gccgagctgt acgcgcgggc ccgggagcta cagcggctga acgaggagct
    13441 gcggcaggcc cacggccgcg agcgccaggt ggccgtcact ttgcaggagg gcatgctcca
    13501 gtcacctgac ctggcccggc accagaacgt cgccgtgcgc tatctgcccg cagccggatc
    13561 gctgaacgtg tgcggcgact ggtatgacgt ggcagacctg accgacggcc gcttctcggt
    13621 ggcggtgggc gacgtcgtcg ggcacggcct ggaggccgcc gccgtcatgg gaatgctccg
    13681 cagcgcactg agcgcggcca tccgcgccct ggagcggccg gcccaggccc ttgaggtact
    13741 ggggctgtac gcccgctcgg tcgagggcgc gctgaacacc accgccgtca aagcgctggt
    13801 cgaccccgca agctgcctga tcatctacag caacgccggc cacccacccc cgatcctggt
    13861 gcacggcgac ggcacctgcg agctgctgga ccgggccacc gacccgcccc tgggggttcg
    13921 ccccctgcac gtcccccgtc cccaggccac ggccatctac agccccggcg acaccctcgt
    13981 gctctacacc gacggcctga tcgaacgccg cgacgaagac atcgacaccg gcctggcccg
    14041 gctgacagac accctcagcc agtcccacca gctcgccccg gagcacctgg ccgacacctt
    14101 gctggcccgc ctcggcctgg ccggcggcgg ccgcgacgac gtcgcactga tcatcgtccg
    14161 gctgtgacca gcgcccttcc tcacccgaga ggtctgcgcg cttggccgcg cagcctgcta
    14221 cctgccccgt tgctatgact ggggatgggt ggcggcctgt acgaggttgt gctccaggcg
    14281 ttcgagggtg gccagggccg cggtccgctc gtcgtcggtg aggcccgctg tcgagaggtc
    14341 ttcgagctgg ctccagacct gctcgacttg gtcgcgcagg gcgtggctgg ccgctgttgg
    14401 ttcgatgagt gaggcgcgtt tgtcggtggg ggaggggcgg cggcggacga agccggcctg
    14461 ctccaggcgc cggacggtgc gggtcatggt ggcggcgtcg gagtccatca gccgtaccag
    14521 gtcggtctga cgctgggggc cgagttccca gaggtgcatc atgaccagtt cctggcccgg
    14581 gtgcaggccg atgcggcgca gcagctgtcc ggcgaacatg cggtgcaggc gggccaggcg
    14641 gaagatcgcg tgactgaccg gtccgccact ggccgcggcc ggcacgggga cggtggcttc
    14701 ttgcccgatc tctgcggggc cgttcccggc ttccggcgtg gactgcgtca tccgtttctc
    14761 acttccctgt ttaggcagtg tgatcctacc ttgctgactg ggctgccgcg gagtcgatgc
    14821 gcatgtacat gcacgtatga gcaggtagtc gcagtgttat gtgcttcaca tcgggaagcg
    14881 agagtgggct gaagggcatt acctgttcgg acaagtgctt tacttgttcg gacaggcatc
    14941 tgctggtgcg ggcaccacag cgtcccggcc gcggagtacc actcacccgg aggaagagac
    15001 catgaccact gctttcgacc cgatcgtcct gggcggcaag cccctggcaa accgcgtcgt
    15061 gatggcgccg atgacccgca gccgcgccta tgggcccggt gccgagccga ccgagctgat
    15121 ggcgacctac tacgcgcagc gcgccagtgc ggggttgatc gtcaccgagg gcatccagcc
    15181 ctcgccggtc ggccagggct accccgacac ccccggcctg cacacccctg ggcaggtgcg
    15241 ggcgtggcgc acggtcaccg acgccgtgca ccgcgagggc ggggtgatct tcgcgcagct
    15301 gatgcacacc ggccggatcg gacacccgag cctgctgccc gacggcctgg tgccggtggg
    15361 gccgtcgccg gtggccgcca aggggcaggt ctacacccac gagggaccga aggagttcgt
    15421 gacgccgaag gagctgagcg aggccgagat ccggcagacg atcgccgact tcgccgcggc
    15481 ggcccgcaac gcggtcgagg ccgggttcga cggcgtggag atccacggcg ccaacggcta
    15541 cctgatccac cagttcctcg cgcccaactc caaccagcgc accgatgcct ggggcggcac
    15601 caccgagggc cggatccgct tcgccgtcga ggtcgtcacg gccgtggccg aggcgatcgg
    15661 cagcgaccgg gtcggcctgc ggatctcgcc cggaaacgtg tacaacgaca tggcggagga
    15721 cgacccggcc gaggtctacg gggcactgct ggagcggctc gccggcctcg aactggccta
    15781 tctgcacctg atggagggcc cggaccgctc cctgaccgcg cggctgcgca aggactggcc
    15841 cggcaccttc gtcctcaacc cgttcaccca ccccgacgtc accggccccg acgcgctgca
    15901 gctcatcgag gacgggagcg cggacatggt ggcctatggg gcgctgttcc tggccaaccc
    15961 cgacctgccc cggcgcctcg ccaccggcgg tccgttcaac acccccgaca aggccacctt
    16021 ctacggcggc gatcaccgcg gctacaccga ctaccccacc cacaccgcct gagcggcgac
    16081 cctgccgagg tggccgcctg gtgagccaca gcgcctgcgg ttcccctctc ctcacctcta
    16141 cccgggagtt ttccgatgtc ccaagcgatt actttctccg cgtacggccc gcccgaggtg
    16201 ctgcaactgt cggaggtcac ccttcctgag cccggccccg gccaggtccg catccgggtg
    16261 cgggccgcct ccgtgaaccc tctcgacatc aagatccgat ccggtctgat ggccggcgcg
    16321 gttcccgccc gctttcccgt gatccccggc ctggacgcgg ccggtgtcgt cgacgccgtc
    16381 ggcgagggag ccgacgcgtc cgtgggggac gaggtcctcg gcggcaccgt cggtggcagc
    16441 tacggcgaac acgccctgct cgatcggccg gtggccaagc ccgctgccct gtcgtgggag
    16501 gtcgccgcct cgctggtcac ggtcggccgg accgcgttcc gtgtccttgg gcagctgggg
    16561 gcgcaagcgg gtcagaccct tttgatccac ggtgccggcg gcagcgtggg caccatcgcg
    16621 gtgcagctgg ccgtcgcccg cggcatcacc gtcgtcggaa cggccggcga gcacgacatc
    16681 gagcgcgtca ccgcgctcgg agccactgcg gtccgctacg gcgacggctg ggtggagcgg
    16741 gtgacggccg cggccgcgca gggagtcgac ttcgtcttcg acgcctccgg agccggcgtg
    16801 cttgccgact ccgtcgccct gaccggcgac agcgcacatg tcatcaccat cgccgacatg
    16861 tccgccgcgc agcacggcgt cgccttcagt gccggaagcg ccgaccaggc cgaagaatct
    16921 ctgccggagc tcgtgcaact ggccgccggc ggccatctcg acgtgcccgt ctggcgcgcc
    16981 tacccgctcg acgaggccgc caccgcccac gccgaccttg aggcccgacg caatcagggc
    17041 aaggtcgttc tccttccctg acgcgccgtg ctgccacctc gccctgtcgt ggagcagaca
    17101 aggcggcaac ggggccgcag ccggaacctc tcggccggct gcggcccatc cccccgcatc
    17161 gccgcacaac cagggacgga gtaccaggac ccgatgacag cgtggcccac gctcacgccg
    17221 ctgaccgctg atgcggacct catcgacgcg ttcgaggtcc tcggccacaa gtggaacggc
    17281 atcatcctcc acactctggc ccccgtcccg cccgctacgg cgaactgcac accgccatcg
    17341 gctcgatcag caccaaggtg ctcgccgacc ggctgcgcga actcgtcgag gccgggctcg
    17401 tcacccacga ccggctcccg cccggacccg ccacctacac cctcaccgcc gagggccgag
    17461 ccctcctacc ggccctggag cagatccgct cctgggcgca ccaccgcacc acctgatccc
    17521 gtccctctcc tccgctccct ctccgaagaa cggaacttcc catgtccgca cgcgcccttc
    17581 ccgaggcgac cctcgcccgc accgtcctgg gttccggccc cggcctcgcc ctcgcgcacg
    17641 gcgccggcag cagcgtggcc ggcacctacg gtccgatcct ggaaggtctg gccgcccacc
    17701 acaccgtcgt cggcgtcgac taccccggca gcggcgacac tccccgctcc gccacgccgc
    17761 tgtcgctgga cgacctggcc gaccagctcg tcgccgccgc cgtcgccgag ggcctggaca
    17821 ccttcgcggt atccggctac tccctcggcg gacccgtggc gatccgtgcg gccgcccggc
    17881 accccgagcg cgtcaccgcc ctcgtactga ccgcgacctt cccccaccgg gacaaccggc
    17941 tcgcgctcgc cctgccgatc tggcgcaagc tggccgagcc gggcaaccgc gcactgctgg
    18001 ccgagttcct cacgatgatg gccctcggcg ccgacgcgct ggaggcgatg ccgaacgagc
    18061 aactccagca ggccctcgcc ttcaccgccg ccagcgtcgc cgacggcacc cccgagcacg
    18121 ccgagctggc cccccgggtc gatgtccgcg aagacctgcc gggcatccag gcacccaccc
    18181 tggtcatctc caccacccag gactggttca cctccacccg cctgcaccgc cagctcgccg
    18241 atgccatccc cggcgcccag ctcgtggaga tccgcaccgg ccacctgccc atggtcgaac
    18301 ggcccgagga atggcagaag ctcatcaccg aattcctcga ccagcacagc gcctgacgcc
    18361 agtcggccct cgcgcccgga ccgcagagct caactgcgat ccgggcgttc ggccttcagc
    18421 acacctgggc gggcagcgga cggcgtctca ggccggcggt cgggcgtgcg acacgaaccc
    18481 tcgctctgtc acgagaccct tccgtttgcc tcggccggcc ggtcaggagg gcgcgccgct
    18541 caggacttcg accttgcccg tgtcgagtga gtaataggcg ccgaccacgg ccagggtgcc
    18601 cttcgccacg agcgggccca ggtcctggtt ggagcgcaga tccgccgcgg tcacggtgac
    18661 ctgggcgcgg gccatggtct cgaccgggtc ggcgccgcct tgccggactg cttgtctgta
    18721 tgccggctcc agggccttga cgatcgcctg caggttgccg ggcagcggtt tgccgtcccg
    18781 aagggacttg tacgccgccg cgacggcgcc gcagcgctga tgcccgagga ccacgacgag
    18841 cggggtgcca ctcgtcatgg gcccgtactc gacggaacct gtgaccaccg ggtcgatcgc
    18901 ctccccgccc gtgcgcatca cgtaaaggtc gcccaggccg gtatcgaaga ggagttcagg
    18961 cggcacgcgg gagtcgatgc acgagaggat cgacccgaaa ggttcctggt gctgggccac
    19021 gagctgacgc cggttcggat cccggtcggg gtggtgaagg tctccgctca cccagcgctt
    19081 gttgccttcc atcagcctcg cgaacgccgc ggcgggtgtg gtcggccggg cctggggcga
    19141 tgcggtggtc gtctgggcgg cccgcgcttc gggcgaagcg ctggtcggcg cggcagtcct
    19201 cgtcgaggag cacccggcaa gggctgccgc ggcgccggca agtccgatgc cgagcagcgc
    19261 tctacggtcc ggatgtccgg ctctgtccat cgctcgtacc tcccgaagcc acgcagacac
    19321 gtcgttgtgt cctgaccagg ctgattgttc gcgtggtccg ggtgacctgg ctgtaggcgc
    19381 aagcgggcgc cggctacgtt gccgccaacg gcccacacca ccgcaccgga cggcagggga
    19441 gtgccggacg tcgggccgcc ctcacggacg gcccggcgcc ggtgcgctcg tcaggtctgt
    19501 cgttcctggc tgcggggggc gtcgccccgg ggccacgcga cgtggatgcc tcgtacgtgg
    19561 cgggcgccag gggcctggcg taccccgagc cggccccgag ggtcaggagt gctgcgcccc
    19621 gcgtgccagc agcatgaagg cgtcgacgag ttcctcggcg ggttgaccgg tggccaggct
    19681 gcgcagttgc agcccgaaga ccagcgcgat ggcactgctc gccagccgct cggcgtcggg
    19741 tacgccgaga ccggtgagga tccgggcggc caactggtcg tatgcggcga aggactccgc
    19801 ggccgcgctg cgcaaccgct cgtcgcggcc ggcctggatg tagagctcga agggtgcgat
    19861 gtgcctgctg tcgaacggcg tacccccggc gacctggctg actgtggcgg cggcccgatc
    19921 ggcgtccatt ccgtcggtct ggcactgggc ggcgagctcg gtgaggcgcc gggtctcctc
    19981 tgcgacgaag tgcagcaggc tctcgcgcag catttcgtgc tgggtcgcga agtggtaggt
    20041 gaccgatccg agggagacgt tggcctcctt ggcgatccgc cggttggtga ccgcggcgac
    20101 gccgtcctca ccgatgatgc gcaggacggc gtcgatgatg ctctgccggg ttccgggggg
    20161 cttggacatg gcagtcattg tgccagcggc gccttcctgg ctggtgggaa gggccgggag
    20221 ccgttccggc cagagcagac cgacctcagg ccgggcggca agcggatcgg tgctccaggc
    20281 ggatcagcga ctccatccgc tgttgacagg gtgacccacc ctccatactg ttcgttcgaa
    20341 cgaacagttt ccgggaggct cgtcttatgc gcatcgcagg atcaaccgtt ctcctgaccg
    20401 gggccgtggg cggtctcggt caggccatcg cccgtgaact ggctgccaag ggagccgagt
    20461 tggtcctctc cgggcgccgt gccgatgccc tggaggcgct cgccgacgag ctcggtgccc
    20521 gagcggtcac cgccgatctc gccgacccgg atcaggtcga ccgcctcgcc gaggcatgcc
    20581 ccgacgtgga catggtgatc gccaacgccg ctctgccctc cagcggcgaa ctgctcgact
    20641 acacgcccga gcagatcgac cgcgcgctga ccgtgaacct gcgcgccccc gtcatgctga
    20701 cccgcgcgct cgcgccccgg atggtcaagg caggtcgggg ccatatcgtc ttcgtcgggt
    20761 cgatctccgg gaaggcggcc accaagtact cctcgctcta cagcgcggcc aagttcggac
    20821 tgcgcggctt cgcgctctcc ctccgccagg acctgcacgg tacgggggtg ggggtgtcgc
    20881 tggtccaacc cggcgccgta cgcgacgcag gcatgttcgc cgtcacgggc gccaccccgc
    20941 cggccggcgt gcgcacggtc tccccgggac aggtggtggc gggagtggtt cgggccgtcg
    21001 agcgcgatct gtgcgaagtc aatgtcgcgc cggtggagtt gaaggtgctc agc
//
