LOCUS       NZ_AP026074            10465 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 plasmid SNP1.
ACCESSION   NZ_AP026074
VERSION     NZ_AP026074.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1073703)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 473817
            Orig. end                         :: 484282
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026074.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10465
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="5"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10465
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10465
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5465](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5465)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(73..507)
                     /locus_tag="HEK616_RS38780"
                     /old_locus_tag="HEK616_78890"
     CDS             complement(73..507)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985426.1"
                     /locus_tag="HEK616_RS38780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78890"
                     /product="ester cyclase"
                     /protein_id="WP_261957935.1"
                     /sec_met_domain="PF07366 (E-value: 1.9e-42, bitscore:
                     139.9, seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAAGATDNTATLHRFHSAVNSGDPEVISKAIDEFVAPDVVFHAP
                     VPMGGTGAEALKRVWEVLVRAFPDIHVTVEETIAEGDKVVSRNTVTGTHRGEYQGLPPT
                     GKTVTYGEIFIMRFTDGRIVEIRGIVDVLTQLRQLGALPS"
     misc_feature    678..693
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.18"
                     /tool="antismash"
     gene            953..1693
                     /locus_tag="HEK616_RS38785"
                     /old_locus_tag="HEK616_78900"
     CDS             953..1693
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (30-143). E-value: 3.4e-07. Score:
                     20.5. Matches aSDomain:
                     nrpspksdomains_HEK616_RS38785_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152071.1"
                     /locus_tag="HEK616_RS38785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78900"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_261957936.1"
                     /transl_table=11
                     /translation="MSVQQYDEIGEAFEGFKGLPLAQYGEVPSFLDMVGDVRGKTVLDL
                     ASGTGFYSREFKRRGAAEVLGIDISGEMVAAARTFEERDPLGVRYEVGDVSQLRPLDAH
                     FDIATGVQLLNYAKDIAAMEGMCRNVRRSLKTGGEFFVLAQTPDYRFDGPSLAKYGFLC
                     EATGEEIETGPRVRITALLDPPISFVGACPRREVYEQCLRAAGFSELTWVPLEVSEAGV
                     RKFGPDFWADLLANPPLEMLRCRA"
     aSDomain        1043..1381
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS38785_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="3.40E-07"
                     /label="HEK616_RS38785_oMT.1"
                     /locus_tag="HEK616_RS38785"
                     /protein_end="143"
                     /protein_start="30"
                     /score="20.5"
                     /tool="antismash"
                     /translation="LDMVGDVRGKTVLDLASGTGFYSREFKRRGAAEVLGIDISGEMVA
                     AARTFEERDPLGVRYEVGDVSQLRPLDAHFDIATGVQLLNYAKDIAAMEGMCRNVRRSL
                     KTGGEFFVL"
     gene            1766..3151
                     /locus_tag="HEK616_RS38790"
                     /old_locus_tag="HEK616_78910"
     CDS             1766..3151
                     /NRPS_PKS="Domain: AMP-binding (37-449). E-value: 9.3e-79.
                     Score: 256.8. Matches aSDomain:
                     nrpspksdomains_HEK616_RS38790_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576529.1"
                     /locus_tag="HEK616_RS38790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78910"
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="WP_261957937.1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-78, bitscore:
                     260.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASMLDGCTPWPEEFVDRYWAAGHWRGNTLDNLLRGWALQYGPRT
                     ALVYGGTRITYANLNRRVGRMAAGFRLRGLRPGQRVVVQLPNVPEFVITAFALLRVGAI
                     PVFCPVAHRAPEVSHLVRVAEAVGYVGPSTYQGFDHTAMAADIAAQGPFLRRVFTLDPP
                     GGSSPYGGLATDPSGCHYFPLSSIDAPPDPAHAQSADQVAFFLLSGTTAPPQLIPRTHN
                     DYAYQARAAAELVSLTENDVYLAALPAEFNLTFGCPGIVGTLSVGGTVVLLDNPEPTAC
                     LAAIERERVTITSVVPAVVQTWLDERPTAQVELSSLRLVQIGGGPLHQATAERIGPEWG
                     CRPQQVFGMAEGPLTLTRPADPDETVLTTQGRPLSPDDEIRIVDIDGKDVPDGETGELL
                     ARGPYTLRGYYRAPDHNARSFTADGYFRTGDLAMRTPDGNLVVTGRLKDVPQRADHKHP
                     PGW"
     aSDomain        1877..3112
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS38790_AMP-binding.1"
                     /evalue="9.30E-79"
                     /label="HEK616_RS38790_AMP-binding.1"
                     /locus_tag="HEK616_RS38790"
                     /protein_end="449"
                     /protein_start="37"
                     /score="256.8"
                     /specificity="substrate consensus: Hpr"
                     /tool="antismash"
                     /translation="ALQYGPRTALVYGGTRITYANLNRRVGRMAAGFRLRGLRPGQRVV
                     VQLPNVPEFVITAFALLRVGAIPVFCPVAHRAPEVSHLVRVAEAVGYVGPSTYQGFDHT
                     AMAADIAAQGPFLRRVFTLDPPGGSSPYGGLATDPSGCHYFPLSSIDAPPDPAHAQSAD
                     QVAFFLLSGTTAPPQLIPRTHNDYAYQARAAAELVSLTENDVYLAALPAEFNLTFGCPG
                     IVGTLSVGGTVVLLDNPEPTACLAAIERERVTITSVVPAVVQTWLDERPTAQVELSSLR
                     LVQIGGGPLHQATAERIGPEWGCRPQQVFGMAEGPLTLTRPADPDETVLTTQGRPLSPD
                     DEIRIVDIDGKDVPDGETGELLARGPYTLRGYYRAPDHNARSFTADGYFRTGDLAMRTP
                     DGNLVVTGRLKDV"
     CDS_motif       2936..3025
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS38790_0001"
                     /evalue="3.00E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="HEK616_RS38790"
                     /protein_end="420"
                     /protein_start="390"
                     /score="19.6"
                     /tool="antismash"
                     /translation="PDGETGELLARGPYTLRGYYRAPDHNARSF"
     gene            complement(3102..3938)
                     /locus_tag="HEK616_RS38795"
                     /old_locus_tag="HEK616_78920"
     CDS             complement(3102..3938)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS38795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEK616_78920"
                     /product="hypothetical protein"
                     /protein_id="WP_261957938.1"
                     /transl_table=11
                     /translation="MAFALVGLKAACSREPSDKKLRAQATSSEAQSRRAAEEQRQTALI
                     QRLAAVDGLEHVLTRFVDSCVRPYKRSVFENRSSPYLMTCSMRAVAYFGVHDDMANVLR
                     RIREAHIATWGPQDGEGRDLPQAAGTVTYALSYHRARGRAPDGSPMPAPTLEATGLRID
                     WDRTTTPVPHRVADPTPCPPPEFGLYQHCSTISETPKSLAAARAHYGTLLSFTLSGSSA
                     SSTDNYFTVPAGSDHPAIAARMRPLICSIRSRIGRRPSPPVTSPVDVCGQPAAGRP"
     gene            complement(4142..4960)
                     /gene="melC2"
                     /locus_tag="HEK616_RS38800"
                     /old_locus_tag="HEK616_78930"
     CDS             complement(4142..4960)
                     /EC_number="1.14.18.1"
                     /GO_function="GO:0004503 - tyrosinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="melC2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="HEK616_RS38800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78930"
                     /product="tyrosinase MelC2"
                     /protein_id="WP_261957939.1"
                     /transl_table=11
                     /translation="MAVRKNQANLTATEKRNFVDAVLELKRAGRYDEFISTHNAFIIGD
                     TDEGERTGHRSPSFLPWHRRFLLQFEQALQSITPSVTLPYWDWTVDRSPRSSLWGDDFL
                     GGDGRSGDGRVTSGPFAFSGGRWPMNLRVDGRTFLRRSLGTGVRDLPTRAEVDRVLAMD
                     VYDAPPWNSASDGFRNNIEGWRGPNLHNRVHVWVGGQMTTGASPNDPVFWLHHCFIDKL
                     WADWQKLHPASGYLPTGDTPDVVDLHDTMKPWNDVTPADMLDHTKFYTYA"
     gene            complement(5001..5465)
                     /gene="melC1"
                     /locus_tag="HEK616_RS38805"
                     /old_locus_tag="HEK616_78940"
     CDS             complement(5001..5465)
                     /codon_start=1
                     /gene="melC1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608161.1"
                     /locus_tag="HEK616_RS38805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78940"
                     /product="apotyrosinase chaperone MelC1"
                     /protein_id="WP_261957940.1"
                     /sec_met_domain="melC (E-value: 7.5e-36, bitscore: 119.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRPTRRQLLHGSAASLAGVVLAAPVAHADRAVARSAGHGHPGAG
                     GHHSDGHAMPEPFDEIYQGRHIQGWPADQGDAHGSRGDHGRRAQRLAGPHDGMDFVVRI
                     DNDDLHVMQNVDGTWISLVNHYEKFSTPHTLARAAVRELQGADLVPVITG"
     gene            complement(6059..7210)
                     /locus_tag="HEK616_RS38810"
                     /old_locus_tag="HEK616_78950"
     CDS             complement(6059..7210)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038577.1"
                     /locus_tag="HEK616_RS38810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78950"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_261957941.1"
                     /transl_table=11
                     /translation="MTVSLSRTAVFTGVLALAAVPALTSCSSASDESTGGGKSAATATT
                     AQAFGGIGALADAAKKEGTLHAIALPRDWANYGALIDGFKKKYGIKVEVENPTGASQDE
                     ISAVTSRKGKDNAPDVLDLGSSFAQSAAQEGLLAPYMVTGFADIPDVQRDSIGRWANDY
                     GGYISFGCDAKRVQTCPTSFKDLLKPQYKGQIALNGDPTKSGSAFGAVYAAALANGGSF
                     DDIQPGMDFFAKLKKSGNYLPIASTPATIERGRTPISIDWDYLNAGYTDEFKSKGLDWT
                     VAVPSDGKFSQYYSQAINKDAPHPAAARLWMEYLYSTEGQNLRLKGYARPALMNAMKKA
                     GTLDKAAAAKLPKVSGTPSFPTETQQSDAKVVISQSWGSAATN"
     misc_feature    complement(7302..7316)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.91"
                     /tool="antismash"
     misc_feature    complement(7543..7557)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     gene            complement(7889..8026)
                     /locus_tag="HEK616_RS38815"
                     /old_locus_tag="HEK616_78960"
     CDS             complement(7889..8026)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS38815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEK616_78960"
                     /product="hypothetical protein"
                     /protein_id="WP_261957942.1"
                     /transl_table=11
                     /translation="MSAVFRGFTPAELAQFRHMCLRLVENQQRIEHYLRTGRPDQPASA
                     "
     gene            8305..8880
                     /locus_tag="HEK616_RS38820"
                     /old_locus_tag="HEK616_78970"
     CDS             8305..8880
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HEK616_RS38820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HEK616_78970"
                     /product="hypothetical protein"
                     /protein_id="WP_261957943.1"
                     /transl_table=11
                     /translation="MTDNYAAIVMSVDAGLLVVGAVQSHGTINTIRERIRAVNQRHAAA
                     MRVIADRLRAGEEPTDAELQRAHGLARKATGDLRRRFERPATVGLMWVIMSFWLVVSVM
                     ATLWWSATEGHGQASWLAEGTLAATALATLVLLAETVLRFWWAGEAATDEWEQEQLQRQ
                     VAAVEMNDDAVSQRVRQRLEEFARGGQG"
     gene            complement(9058..9789)
                     /locus_tag="HEK616_RS38825"
                     /old_locus_tag="HEK616_78980"
     CDS             complement(9058..9789)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943593.1"
                     /locus_tag="HEK616_RS38825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_78980"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_261957944.1"
                     /transl_table=11
                     /translation="MKCAVVGGTGLIGSRVVENLNAAGHQAVPHALTTGVDIITGQGLD
                     AAVEGADVVINLTNSPTFDDASLAFFQTSMDNLLAASEKGRVRHFVILSIVGVDQVPDL
                     DYYRAKTLQENILKAGPIPYSIVRATQFMEFMDATLAMTTDGDTVRLPRTPVQPIAAAD
                     VSAIVAEVAAGSPLNGVLNIAGPDVYPLDELGRLTLTAKGDGRSVVTDNSAGMFAAAHG
                     DVLTAPNDARIAPTHYTDWLS"
ORIGIN
        1 ccgtggtgaa ccctgccggc gacgccggtc acccgcggta ggtcgttgcg gcgttgctgc
       61 tcatgggcct cgtcaggacg ggagggcgcc gagctgacgt agctgcgtga ggacgtcgac
      121 gatgccccgg atctcgacga tccgcccgtc ggtgaagcgc atgatgaaga tctcgccgta
      181 ggtgacggtc tttccggtgg gcggcaggcc ctggtactca ccccggtggg ttccggtcac
      241 cgtgttcctg gagacgacct tgtcgccctc cgcgatcgtc tcctcgacgg tgacgtggat
      301 gtcggggaac gcgcgcacga gcacctccca cacccgcttc agggcctccg ctcccgtccc
      361 gcccatcggt accggcgcgt ggaagaccac gtccggtgcg acgaactcgt cgatggcctt
      421 cgagatgact tccgggtcgc cgctgttcac ggcggagtgg aagcggtgga gcgtcgctgt
      481 gttgtctgtt gccccggctg ccgacatgca ccttctcctc tgctctgggc cttcgccgcg
      541 tgctggacgg cgtctgagca gtaagacggc acggcctccg gaaatgtgac acgacgcgcg
      601 gacgacaggc cagggagtcg ctgccatggc cggcggaccg gggatgacga gtgggcctga
      661 gatgactggc ggaccggatg accggcaggt cgatgtgcac ggcaggccgc cgagccaggc
      721 gaccggaccg aggtcgagga gcggcgtcga agaacgcacc gaggtcgtcc atcacccgct
      781 cggatccgta cgcccgcccg tgcacgagac cgggacggcg tccggatctt tgtggcttct
      841 gccagtacga cgggccgccg gggtgcgggg agactgcccg gcagaggccg cggcgtgccc
      901 agggagggag gccgaggccg aggacacgac gaattacggg gagggcggca cggtgagcgt
      961 gcagcagtac gacgagatcg gtgaggcatt cgagggcttc aagggcctgc cgttggcgca
     1021 gtacggggag gtgcccagtt tcctggacat ggtcggggac gtgcgtggca agacggtcct
     1081 cgacctggcc tccggcaccg gcttctacag cagggagttc aagcggcgcg gcgccgcgga
     1141 ggtgctcggc atcgacatct ccggtgaaat ggtcgccgcg gcaaggacgt tcgaggagcg
     1201 cgatccgctg ggcgtgcgct acgaggtcgg cgatgtctcc caactacggc ccctcgacgc
     1261 gcacttcgac atcgccacgg gtgttcagct gctcaactac gcgaaggaca tcgcggccat
     1321 ggaggggatg tgccgcaacg tgcgccggag cctgaagacc ggtggtgagt tcttcgtgct
     1381 cgcccagacc cccgactacc gcttcgacgg gccgtccttg gccaagtacg gctttctctg
     1441 cgaggcgacc ggcgaggaga tcgagaccgg accgcgggta cggatcacgg cgctgctcga
     1501 cccgccgatc tcgttcgtcg gcgcctgtcc gcgccgtgag gtctacgaac agtgtctgcg
     1561 ggcggccgga ttcagcgagt tgacctgggt cccgctggag gtgtccgagg ccggcgtgcg
     1621 caagttcggt ccggacttct gggcagacct cctcgccaac cctccgctgg aaatgctgcg
     1681 ctgccgcgcc tgacgaccgg ccgaacgtcc cgcggaacac ggcccgcacc gctcccgatg
     1741 cagaatccgg cacagaagaa agcgaatggc atccatgctc gacggctgca cgccgtggcc
     1801 cgaggagttc gtcgaccgct actgggcggc cgggcactgg cgtggcaaca ccctggacaa
     1861 cctgctgcgg ggctgggccc tgcagtacgg accgcggacc gcgctcgtgt acggcggcac
     1921 ccgcatcacg tacgcgaacc tgaaccggcg cgtgggtcgc atggccgccg gcttccggct
     1981 gcgtggcctc cggcccgggc agcgggtcgt cgtccagcta ccgaacgttc ccgagttcgt
     2041 catcactgcc ttcgcgctcc tgcgcgtcgg cgctatcccc gtgttctgcc cggtcgcgca
     2101 ccgcgctccc gaggtgtccc acctcgtgcg ggtcgccgag gccgtcggct atgtcggccc
     2161 ctcgacgtac cagggcttcg accacaccgc gatggccgcg gacatcgcgg cccaagggcc
     2221 tttcctgcgg cgggtgttca ccctcgatcc gccgggcggc tcgtccccgt acggcggcct
     2281 cgccaccgac ccctcgggct gccactactt cccactgagc tccatcgacg ccccgcccga
     2341 cccggcgcat gcgcaaagcg ccgaccaggt ggcgttcttc ctgctctccg gaaccaccgc
     2401 cccgccccag ctcattccgc ggacccacaa cgactacgcc tatcaggcgc gtgccgctgc
     2461 cgagctggtg tcgctcaccg agaacgacgt gtatctcgcc gcgctgcccg ccgagttcaa
     2521 cctcaccttc ggctgcccag gcatcgtcgg caccctctcc gtcggcggca ccgtcgtgct
     2581 gctcgacaac ccggaaccca ccgcatgcct tgcggccatc gaacgggagc gggtcaccat
     2641 cacctcggtg gtgcccgccg tcgttcagac ctggctcgat gaacgtccca cggcacaggt
     2701 cgagttgagc agcctgcgtc ttgtgcagat cggtggcggg cccttgcacc aggcgaccgc
     2761 cgaacggatc ggccccgaat ggggctgtcg cccgcagcag gtcttcggca tggccgaggg
     2821 cccgctcacc ctcacccggc ccgccgatcc ggacgagacc gtgctgacca cccagggccg
     2881 cccgctctcg cccgacgacg agatccgcat cgtcgacatc gacggcaagg acgtgcccga
     2941 cggggagacc ggcgaactcc tcgcccgcgg tccgtacacc ctcaggggct actaccgggc
     3001 acccgaccac aacgcacgct ccttcaccgc cgacggttac ttccgcaccg gcgacctcgc
     3061 catgcgcacc ccggacggca acctggtggt gaccggccgc ctcaaggacg tcccgcagcg
     3121 ggctgaccac aaacatccac cgggctggtg acgggcgggg acggccgccg accgatcctg
     3181 gaacggatcg agcagatcag cggccgcatc cgcgcggcga tggccgggtg gtcacttccg
     3241 gcgggcacgg tgaagtaatt gtccgtggag gaggccgagg agccactgag ggtgaagctc
     3301 aggagggttc cgtagtgggc ccgcgcggca gccaggctct tcggggtctc cgaaatggtc
     3361 gaacagtgct ggtagaggcc gaattcgggc ggagggcagg gggtcggatc cgcaacccgg
     3421 tgggggaccg gcgtggttgt ccggtcccag tcgatccgca ggcccgtggc ctccagtgtg
     3481 ggggccggca tggggctgcc gtccggggcc cggccgcgag cacggtggta gctgagcgcg
     3541 taggtcaccg tgccggccgc ttgtggcagg tcccggcctt cgccgtcctg aggcccccag
     3601 gtcgcgatgt gtgcctcacg gatccggcgt agcacgttgg ccatgtcgtc gtggacgccg
     3661 aagtaggcaa cggcccgcat gctgcaggtc atgaggtacg gggaactcct gttttcaaag
     3721 accgagcgtt tgtagggcct gacgcaggag tcgacgaacc gtgtgagaac gtgctccaga
     3781 ccatcgacgg cggccaggcg ctgaatgagt gccgtctgcc gctgctcttc cgccgccctg
     3841 cgggactgtg cttcggacga cgtggcctgc gcccgcagtt tcttgtccga aggctcgcgt
     3901 gagcacgccg ctttcagtcc taccagtgcg aatgccaaca gcaatccgcc ggcttgagag
     3961 caccgcccgc tgatgcggtg ccaccgggcc gccacagtcc ccttcatctg cgcaacggcg
     4021 cggcggcagg ccatggagtc gctgccgccg cgccggatct cgcccccgga tgcggactgc
     4081 ctctgcaggg aactcgcccg cgatgcaacg gacgtgaggg gctgtcgctg tcaggtacct
     4141 gtcatgcgta agtgtagaac ttggtgtggt ccagcatgtc cgccggtgtc acgtcgttcc
     4201 acggcttcat cgtgtcgtgg aggtcgacga cgtcgggggt gtcgccggtc ggcagataac
     4261 ccgaggcggg gtgcagcttc tgccagtcgg cccagagctt gtcgatgaag cagtggtgca
     4321 gccagaagac cgggtcgttg ggggaggcgc cggtggtcat ctggccaccg acccagacgt
     4381 gcacgcggtt gtgcaggttg gggccgcgcc agccctcgat gttgttgcgg aagccgtcgg
     4441 aggcgctgtt ccacgggggc gcgtcgtaga cgtccatggc gagcacccgg tcgacctcgg
     4501 cccgggtcgg caggtcgcgg acgccggtgc ccagggagcg gcgcaggaac gtccggccgt
     4561 ccaccctgag gttcatcggc cacctgccgc cgctgaaggc gaacgggcca gaggtcaccc
     4621 ggccgtcgcc gctccgcccg tcacctccga ggaagtcatc gccccacagg gagctgcggg
     4681 ggctgcggtc gacggtccag tcccagtagg gcagggtgac cgagggggtg atgctctgca
     4741 gggcctgctc gaactgcagc aggaacctgc ggtgccaggg gaggaaggac ggcgaccggt
     4801 ggccggtccg ttccccctcg tcggtgtcgc cgatgatgaa ggcgttgtgg gtgctgatga
     4861 actcgtcgta acggccggcg cgcttgagct ccagcaccgc gtcgacgaag ttccgcttct
     4921 cggtggcggt gaggttcgcc tggttcttcc taactgccat gtcgaggact cctcggtggg
     4981 gggcgggcag gggcgcggcc tcagccggtg atgacgggga ccaggtcggc gccctggagt
     5041 tcccgtacgg cggcgcgcgc cagggtgtgc ggggtgctga acttctcgta gtggttcacc
     5101 aggctgatcc aggtgccgtc gacgttctgc atcacatgca ggtcgtcgtt gtcgatgcgg
     5161 acgacgaagt ccatgccgtc gtgcgggccg gcgaggcgct gcgcccgccg tccgtggtct
     5221 cctcggctgc cgtgcgcatc gccctggtct gccggccagc cctgtatgtg gcggccctgg
     5281 tagatctcgt cgaacggctc gggcatggcg tggccgtcgc tgtggtgccc gcctgccccg
     5341 gggtgtccgt ggccggcgga tcgtgcgacg gcgcggtcgg cgtgggcgac cggtgcggcc
     5401 agcaccactc ctgcgagcga tgcggcactg ccgtgcagca gctggcgccg ggtgggacgc
     5461 gacatcttcc actcctctgg tcgggcctcg gctcgcctcc ggggcgctgc cgcgccggag
     5521 ggcgggagcc ggcgtgccga tcgtcggtac gcgccgaccc atgctgacag ggacgatcag
     5581 ggcatcaagt cggcccgcgt gatcgggcca gagtggaaca acacccgtac cgaggtacgg
     5641 gattcaacgg cgttgatcgt ccttgcgcgg cggtcgggaa gctctccgga agcggatatt
     5701 ctgcggtttc ggctgccatc cgccgcagat atttttctcc gcgcagcaat ccgtgatgcc
     5761 gtacgcggcc cgaactcggc gcaaacggag ggggtttggg tgctcggggg tttgcgggcg
     5821 gcacccgttc gtcttccgcg gtccggcgcc ggaccggtgc ggacgtggcc gcaagggggc
     5881 gccctgtgcg cgagcgggcg gacggtgacc ggaggggtcc ggctggcggt cgagagtcat
     5941 gcggagaccg gcaggtacca cctttgggtc gtcggcgatg aggccggtca gccggccgcg
     6001 cggtgccgcc gctgggagga agcggcgggc accacgtcgg tccgggtgcg ggtcgcggtc
     6061 agttggtggc ggccgagccc cagctctggc tgatgacgac cttggcgtcg ctctgctggg
     6121 tctcggtcgg gaagctcggg gtgccggaga ccttcgggag cttcgccgcc gcggccttgt
     6181 cgagcgtgcc ggccttcttc atggcgttca tcagagccgg gcgggcgtat cccttgagtc
     6241 tgaggttctg accctcggtg ctgtagaggt actccatcca caggcgcgcg gccgcggggt
     6301 gcggcgcatc cttgttgatg gcctgcgagt agtactggga gaacttgccg tccgagggaa
     6361 ccgcgaccgt ccagtcaagg cccttggact tgaattcgtc ggtgtacccg gcgttgaggt
     6421 agtcccagtc gatgctgatc ggcgtccggc ccctctcgat ggtggccggg gtggacgcga
     6481 tgggcaggta gttgccgctc ttcttcagct tggcgaagaa gtccataccg ggctggatgt
     6541 cgtcgaagga gccgccgttg gccagagcgg ccgcgtacac ggcgccgaag gccgaaccgg
     6601 acttggtggg gtcgccgtta agggcgatct gccccttgta ctgcggtttg aggaggtcct
     6661 tgaagctggt cgggcaggtc tgcacacgct tggcgtcgca gccgaaggag atgtagccgc
     6721 cgtagtcgtt cgcccagcgg ccgatcgagt cccgctggac gtcgggaatg tcggcgaaac
     6781 cggtgaccat gtacggcgcg agcagcccct cctgggcggc gctctgtgcg aaggagctgc
     6841 cgaggtcgag gacgtcgggc gcattgtcct tgcccttgcg cgaggtgacc gcgctgatct
     6901 cgtcctggct ggcgccggtc gggttctcca cctcgacctt gatgccgtac ttcttcttga
     6961 agccgtcgat cagggcgccg tagttggccc agtcgcgggg cagtgcgatc gcatgcagcg
     7021 tgccctcctt cttggccgcg tcggccaggg cgccgatgcc gccgaaagcc tgggccgtgg
     7081 tcgcggtggc cgcgcttttt ccgcccccgg tcgactcgtc ggaagccgag ctacaggagg
     7141 tgagggcggg tacggcggcg agggcgagga cgccggtgaa aacggcagtc cttgacaggg
     7201 acacggtcac ggtctctcca gaggggtacg cacagagcat tgaggggata catgcggggc
     7261 cacacagggg atgcacgtgg ggggtggcac ctgtccgacg accttgtctg aacaagttgg
     7321 gcctcagtag gcctaactat ggtgtcctac gggtaaacat tggtaaaact aatgactccg
     7381 attccctcac aatcgcagct cggaggggtc actgcggcag ctcgcccaga ctggccgctc
     7441 tacacacttt gcgcacaatt gcgggcgaga gggagcgcgg cggcacggcc tgcgcaaggt
     7501 cgccgcgtag tccttgcccg ccactggacc gacctggccg cgatcgacga cgtcgatgac
     7561 tggttgccct cgtcggtgcg gctcgggtgg tgatcccgag atcctggacc agccgtactg
     7621 agccacctgt cgtcacatat ggggcgcggc ctgatcgcga tccggggcga ttgcggccat
     7681 aacggcctgc cattgtgagc ggcggcggcc gcgacgtctc cgcggaaccg ggcgtcgtga
     7741 taggccctga cggtacgccg ggtcggtgaa tcggtggccg gtgaatcggt ggccggtgaa
     7801 tcggtggccg gtgaatcggt ggtcgcggaa tcggcggtca tggcgtcctc gggagccgtc
     7861 ggtgcgggaa agggatcgat cggccggttc aagcagaggc cggctggtcg ggccgccccg
     7921 tgcggaggta gtgctcgatc cgttgctggt tctcgaccag gcgcaggcac atatgccgga
     7981 attgggccag ttcggccggg gtgaatcccc ggaagaccgc agacatggcg cgttgtgtcg
     8041 gctggcggaa ggactccatc cagtcgcgtc cggccggtgt gatccgcact cggaccgacc
     8101 ggcggtcgtg ccggtcctgg acccgctgaa cgaatggtcg cgctccaggg tgtcgaccag
     8161 gccggtgacg ttgcgggagc tcaccccgcc ggcgcgcgcc agttcgccga tgctcaagcc
     8221 gacaggagca ctcctgttgg ctgccccggg cggcaggcgc ttctgtaggc tcccttgtac
     8281 cggggggagc tcatgcgctt tgtcatgacc gacaactatg ccgcgatcgt catgtccgtc
     8341 gacgcgggac tgctggtggt gggtgcggtg cagtcccacg gcaccatcaa caccatcaga
     8401 gaacgcatca gagcggtgaa tcagcgccat gcggcagcga tgagagtgat cgccgatcgg
     8461 cttcgtgcgg gtgaagagcc gacggacgcc gaactgcaac gggcccatgg tttggcccga
     8521 aaggccaccg gagacctgcg gcggcggttc gagaggccgg caaccgtggg cctgatgtgg
     8581 gtcatcatgt cgttctggct cgtggtctcc gtgatggcga cactctggtg gtcggcaacg
     8641 gaaggacatg gccaagcctc atggctggcc gagggcacgt tggcggctac ggccctcgcc
     8701 accctcgtcc tgctggcaga gactgtcctc cggttctggt gggcgggcga agcggcgacg
     8761 gacgaatggg aacaggaaca actccaacgc caggtcgccg cagtcgagat gaacgacgac
     8821 gcggtgtccc agcgggtgag gcaacggctc gaggagttcg cccggggcgg ccaaggctga
     8881 ggctgccgag gccgctcagt cacaggctgt tcagccgcag aggccggcct cggtcacgta
     8941 ctggtccgcg atctgcccac gccaggtgac gcatgcgcag cgcgcgggga tgtacaccgg
     9001 cccggcatag gacgtgtagg cgcccggcgg aacgagggcg gaacgggaag ggccctttca
     9061 ggacagccaa tcggtgtagt gggtgggggc gatgcgggcg tcgttcgggg cggtgaggac
     9121 gtcgccgtgg gcggcggcga acatgccggc gctgttgtcg gtgacgacgg agcgcccgtc
     9181 gcccttggcg gtcagggtga gtcggccgag ttcgtcgagg gggtagacgt cgggacctgc
     9241 gatgttgagc acgccgttca gcgggctgcc tgcggcgact tcggcgacga tggcggacac
     9301 gtccgcagcg gcgatcggct ggacgggtgt gcgtgggagg cggacggtgt cgccatcggt
     9361 ggtcatggcc agggtcgcgt ccatgaactc catgaactgc gtggcgcgga cgatggagta
     9421 cgggatcggt ccggccttga ggatgttttc ctgaagcgtc ttcgcccggt agtagtccag
     9481 atcggggact tggtccacgc cgacgatcga gaggatgacg aagtgtctga cccggccctt
     9541 ctcgctcgcg gcgagaaggt tgtccatcga tgtctggaag aaggcgagcg aggcgtcgtc
     9601 gaaggtcggg gaattcgtca ggttgatgac gacgtcggcg ccctccacgg ccgcatccag
     9661 cccctgacca gtgatgatgt ccacgcctgt ggtcagggcg tgcggtacgg cctgatgccc
     9721 ggccgcgttg aggttctcca ccacccgtga cccgatcagc ccggttccgc cgacgactgc
     9781 gcacttcatg gcccaacctt ctttccgtgg aattgcccga tatgcgttgc atgcggcctt
     9841 agctgcattt cgtccatact cgcactgggg cgccgttgcc gcggtgatca cggcacgctc
     9901 gccctcgctc gcgtcgtcta ctcctggtgg gccgagccga agtcgaattc tgcgattcct
     9961 gcccgatatc gggcagaaat atggacgttg cgtggttccc ggcctagggt gatcgctcat
    10021 gatcaccctg gaagcaatgg ggactttcgc cgtgatagtg ggagttctga ccctcactcc
    10081 tggactggac accgcactca tcctgcgcac cgcggcattc gggcaccgtc gtcgtgcctg
    10141 gggcgtggtc ctcggcatcc aaagcggcac gttggtctgg ggcgtgctca cctcgctcgg
    10201 tgtgaccgcg ctgctgacgg cgtctcacct cgcgtacacc actcttcgtt ggatgggagc
    10261 cgcgtacctg gtgtggatgg gcgccaagat gctgtgggcc acctggcgcc gctcggcgag
    10321 ggagagtcca cagacagcgg agagcgggag cgagggcggg accgggggtg ggcccagggc
    10381 caggaccggc gccggcgaca ccctgctcgg tggctggcgg caagggatgc tgaccaatct
    10441 cctcaacccg aagatgggcg ccttc
//
