LOCUS       NZ_AP026073            10999 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5723002
            Orig. end                         :: 5734001
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10999
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="21"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10999
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10999
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5999](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5999
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    9..24
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    complement(1000..1013)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.82"
                     /tool="antismash"
     gene            1170..2228
                     /gene="sepH"
                     /locus_tag="HEK616_RS25545"
                     /old_locus_tag="HEK616_52180"
     CDS             1170..2228
                     /GO_process="GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /gene="sepH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093446.1"
                     /locus_tag="HEK616_RS25545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52180"
                     /product="septation protein SepH"
                     /protein_id="WP_261955272.1"
                     /transl_table=11
                     /translation="MPELRVVAVSNDGTRLVLKAADSTEYTLPIDERLRAAVRNDRARL
                     GQIEIEVESHLRPRDIQARIRAGASAEEVAQLAGIPVDRVRRFEGPVLAERAFMAERAR
                     KTPVRRPGENTGPQLGEAVAERLLLRGADKDSVQWDSWRRDDGTWEVLLVYRVATEPHS
                     ASWTYDPPRRLVQAVDDEARALIGEADDTPEPSFPFVPRIARLPRDRPLDRTLDRHSER
                     GERGAQAGGEDDARGAEEATGERERDSLTSLLEAVPSFRGDMVVPESAKAPQADEESEA
                     EVEEPPAPAASAGAGSAYADVLMPRAVAGHRDRLTGTTDRQAEADGVRPGRRAAVPSWD
                     EIVFGTRRKKPE"
     gene            complement(2325..3164)
                     /locus_tag="HEK616_RS25550"
                     /old_locus_tag="HEK616_52190"
     CDS             complement(2325..3164)
                     /EC_number="2.8.1.-"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602638.1"
                     /locus_tag="HEK616_RS25550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52190"
                     /product="sulfurtransferase"
                     /protein_id="WP_261955273.1"
                     /transl_table=11
                     /translation="MNVIITATELASELAQSAPPVVLDVRYQLGGPPGRPEYEAGHVPG
                     AVYVDLNAELAAPPGPAGRHPLPDLGVFAKAMQAAGVRADHPVVVYDGGQGWAAARAWW
                     LLRWSGHQDVRVLDGGLAAWRAAGGELSTAQPAPEPGDFTPVPGALPLLTADEAAALAR
                     RGVLLDARAGERYRGEVEPLDRVAGHIPGAVSAPTTENVAEGGTVFRDRAELAERFAAL
                     GATPDAEVGVYCGSGVSAAHQVLALAHAGIPAALYVGSWSEWSADPARPVATGPQPG"
     misc_feature    complement(3149..3163)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.55"
                     /tool="antismash"
     misc_feature    complement(3150..3165)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.93"
                     /tool="antismash"
     misc_feature    complement(3254..3271)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.15"
                     /tool="antismash"
     gene            3411..4199
                     /locus_tag="HEK616_RS25555"
                     /old_locus_tag="HEK616_52200"
     CDS             3411..4199
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571513.1"
                     /locus_tag="HEK616_RS25555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52200"
                     /product="VOC family protein"
                     /protein_id="WP_261955274.1"
                     /transl_table=11
                     /translation="MTTEAATRRMPGAPCWVSLLAHSLTATQEFYGALFGWEFRPGPQH
                     FGPYARAFLDGREVAGVGELAPDRHLPVAWTTYLASDDADVTAEQVRCCGGTVGVGPLD
                     ADHDAGRMAIASDPQGAIFGIWQGRTMMGTAVTGEPGTPVWNELVTQETGAVSPFYEHV
                     FGFEAEPAPADGTDSGSDYLTLRLKGHPVAGIHGVGNAPLRDRGPHWMTYFAVPDTDAA
                     ARRVTELGGHVLQPAHESAHGRAATVADSEGAVFTIVQQR"
     gene            complement(4248..4892)
                     /locus_tag="HEK616_RS25560"
                     /old_locus_tag="HEK616_52210"
     CDS             complement(4248..4892)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988493.1"
                     /locus_tag="HEK616_RS25560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52210"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_261955275.1"
                     /transl_table=11
                     /translation="MAKQERAIRTRKACLVAAAEIFNEYGYEAATIAAIIDRAKLTRGA
                     LYFHFTSKEELARGVLDEAVTSEGLVPQVLKLQEWVDISLLLAYRLPREPLLSASIRLS
                     VDSKARSLFGTRWPDWIVLGRELLIEAQRRGELLPHVDPTDTARLFVGAWTGVQLVAEA
                     VPHGPDLSAEISRLLELFLPNVAVPGVLARLDTSPFRAERLFEARSAQPVS"
     gene            5001..5999
                     /locus_tag="HEK616_RS25565"
                     /old_locus_tag="HEK616_52220"
     CDS             5001..5999
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="HEK616_RS25565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52220"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_410011254.1"
                     /sec_met_domain="AfsA (E-value: 8.3e-18, bitscore: 63.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEAAARIWELPQDRGSPGPVLRVFGAQDRVDAVDPTVVPHEYAH
                     LHHTDRVFITGWNRRGPGAFSLTARWPAAQGDGRCDPRVLAQTFRQSGLVIAHAEYGVP
                     LSHHMLLHDLNFTANPDFRLPEGRSTVLDVEVTVAQTRRSGRCASALGMVVRIHQGEVT
                     VAQAESEFAWSSPAVYRRLRGEHLGVDWGAWPLPAPVAPELVGRVDEADVVLAAGDRPQ
                     RWQLRNDPGNTWLFDHPVDHVPGLALLEAAYQAAHATVAPAHFAPATVTSNYERYVEFD
                     EPCWLETELRPATPSGRLTVLVTGTQGGRTVFRAGLSGAACQGSGAGRLTG"
     misc_feature    5868..5870
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5989..6768)
                     /locus_tag="HEK616_RS25570"
                     /old_locus_tag="HEK616_52230"
     CDS             complement(5989..6768)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (14-176). E-value: 2.4e-15.
                     Score: 48.8. Matches aSDomain:
                     nrpspksdomains_HEK616_RS25570_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047022.1"
                     /locus_tag="HEK616_RS25570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52230"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_261955277.1"
                     /sec_met_domain="adh_short (E-value: 3.5e-36, bitscore:
                     123.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.9e-30, bitscore:
                     105.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSDLLSGKLLSGKIVLVTGASSGIGAAAARVFSDEGATVVLVAR
                     REERLAELAGALRAEGAEAAYVAADVSVAEDAARAVDFAVRTYGRLDAAFNNAGIGGDR
                     TPLHLMSDDVYDAIMDTNVRGVWNCLRHEIAAMLPQGAGTIVNNSSVAGLIAIPAAAPY
                     IASKHAVVGLTRAAADEYARQGIRVNAVAPGTTRSEITTDWFARNPGLEEMANSATPQG
                     RTAAPEEIAAAAAWLLSDRCPFLTGTVLPVDGGLVNQ"
     CDS_motif       complement(6214..6357)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS25570_0002"
                     /evalue="1.50E+01"
                     /label="PKSI-KR_m1"
                     /locus_tag="HEK616_RS25570"
                     /protein_end="185"
                     /protein_start="137"
                     /score="-1.6"
                     /tool="antismash"
                     /translation="LPQGAGTIVNNSSVAGLIAIPAAAPYIASKHAVVGLTRAAADEYA
                     RQG"
     aSDomain        complement(6241..6726)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS25570_PKS_KR.1"
                     /evalue="2.40E-15"
                     /label="HEK616_RS25570_PKS_KR.1"
                     /locus_tag="HEK616_RS25570"
                     /protein_end="176"
                     /protein_start="14"
                     /score="48.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="IVLVTGASSGIGAAAARVFSDEGATVVLVARREERLAELAGALRA
                     EGAEAAYVAADVSVAEDAARAVDFAVRTYGRLDAAFNNAGIGGDRTPLHLMSDDVYDAI
                     MDTNVRGVWNCLRHEIAAMLPQGAGTIVNNSSVAGLIAIPAAAPYIASKHAVVGLTRA"
     CDS_motif       complement(6631..6723)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS25570_0001"
                     /evalue="7.30E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="HEK616_RS25570"
                     /protein_end="46"
                     /protein_start="15"
                     /score="12.2"
                     /tool="antismash"
                     /translation="VLVTGASSGIGAAAARVFSDEGATVVLVARR"
     misc_feature    complement(6724..6744)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            complement(6805..7473)
                     /locus_tag="HEK616_RS25575"
                     /old_locus_tag="HEK616_52240"
     CDS             complement(6805..7473)
                     /EC_number="2.7.1.21"
                     /GO_function="GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018520599.1"
                     /locus_tag="HEK616_RS25575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52240"
                     /product="thymidine kinase"
                     /protein_id="WP_261955278.1"
                     /transl_table=11
                     /translation="MSELVFFSGTMDCGKSTLALQIGHNRAARGLEGVIFTRDDRAGEG
                     RLSSRLGLVTDAVEVEEGMDLYAYLVAHLSRGGRADYVIVDEAQFLAPGQIDQLARVVD
                     DLDLDVFAFGITTDFRTRLFPGSQRLIELADRIEALQVEALCWCGARATHNARTVDGAM
                     VVEGAQVVVGDVRQTTAEVGYEVLCRRHHLRRMTSATARAGALSPDVLPVAPTPQDATG
                     "
     misc_feature    complement(7448..7464)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(7451..7466)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.68"
                     /tool="antismash"
     gene            complement(7595..8785)
                     /locus_tag="HEK616_RS25580"
                     /old_locus_tag="HEK616_52250"
     CDS             complement(7595..8785)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093442.1"
                     /locus_tag="HEK616_RS25580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52250"
                     /product="alkaline phosphatase family protein"
                     /protein_id="WP_261955279.1"
                     /transl_table=11
                     /translation="MVDIAPAWPEPEPLDPLGAPVPQYGTGSLADLLPTLAAGLGMTEL
                     ATGMDLAPADRACVFLIDGLGWDLLRAHPEEAPFLTSLLGTSFNGSGRPLTAGFPSTTA
                     TSLASVGTGLPPGVHGLPGYTCQDPATGELMNQLRWYPWTDPYAWQPYPTVFQLADAAG
                     IHTCQVSAPDFQHTPLTKIALSGGSFHGRLSGEERMDLAAEQLGAADRSLVYTYYSEVD
                     GKGHRYGVNSDAWRGQLMYVDRLAQRLAEQLPPRSALYITADHGMIDIPFGPESRIDFD
                     EDWELRAGVRLLGGEGRARHVYAHPGAAADVLAVWREVVGDQMWVASREEAIAAGWFGP
                     YVDDRVRDRIGDVVAAAHADMVIIATEREPRESEMVGMHGSMTSMEQLVPLLEVRT"
     gene            complement(8785..9378)
                     /locus_tag="HEK616_RS25585"
                     /old_locus_tag="HEK616_52260"
     CDS             complement(8785..9378)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571516.1"
                     /locus_tag="HEK616_RS25585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_52260"
                     /product="DUF5998 family protein"
                     /protein_id="WP_030417303.1"
                     /transl_table=11
                     /translation="MAKTGTTTQGLRAAIERSGYYPTLVAEAVQAAVGGEPVVSYLVHQ
                     ETTFDANEVRRHVTVLVLTGTRFIVSHTDEQAADATSPSPYATTSTESVKLGRISSVVL
                     SRVVANPESYTPGQLPREVVLTIGWGAVARLDLEPAACGDPNCEADHGYTGSSTADDLS
                     LRVSEAGDGPDSVRETLAFAQALSEATAATTTPR"
     misc_feature    9694..9708
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.68"
                     /tool="antismash"
     misc_feature    10600..10614
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 ccccgcccat ggcgagggcg aacaccgcga gcgcgccgag tgccggccgc ttccagccgc
       61 gcagccgcgt gccgtacgtg gtggcgctga cctcctcggg ccggccgacg cgcgacccgg
      121 ccgggggcag gacggcatcc gggcgggcgg gcgcctgcac cggtgtcatg gtggggacgt
      181 cgaggcgcgg caccatttga gtccggtcga tcgggtgggc gttcgcagga tcgcgccgga
      241 gggcctgcac ggcccggggg atcacctgcg tacgggccgt gtcgggcccg gaggtcttgg
      301 cggcgacgga ggtgacgtgg tcctccgtgc cctgcttgtc gaaggtgggc agcaccatcg
      361 tggggtcggg ccgccctgtc tgccgggtgg tggatcgagt gcgggaggag gagcggcgcg
      421 gcctcggccg ggtgctgacc gaagcctcct tgatctgctc gccggtacgc cggaagaggt
      481 gctggaatat cgacccgccg gtcgtggcca cgacactgac cagacccgca ccgatgatgg
      541 ttccgtacac accgagttga ccggcgagat acgcggcgac ggccgcggcg agtgcactgc
      601 cggacacctg agcgacgctc aggtctatcc gcttctcctt cttcttcgtg cctgtttcgg
      661 tttcttcgtc catctccaac ctttgatagc cccttcgatc catcttgcac ggagaagtga
      721 ccaattggcg gagtcaaagg ttccgaatct ggggaatatg tgaaactcgc cacccgtgcg
      781 tcaggtcgag agatcgacaa ggtgaccgac ccccgctgtt cccccacaag ttcggcggcg
      841 cggcggtcca cctcggtggt ccgaatggag tactgtgacg aaccctggcc ccggttcccg
      901 tccggctgcc cggaacccat gggaggggcc agatgacggc actcaccccg gggcgccgcc
      961 gctcggcacg ggtgatcggc gacagggcaa gcggcatggt cactgagcgt tgcgaactgg
     1021 taaccgtgtc ataacggcgt ttgagggcca agcctcgaca cgccgggcaa ctcggcaatg
     1081 ttgtggcagg ctgcacccgg gcaggccaca ctcgactagc ggaagcagcg acgcacgtga
     1141 cgtcggcagg caccacccgg gaggtcccca tgcccgaact gcgtgtcgtg gccgtctcca
     1201 acgacggcac acggctggtg ctcaaagctg cggacagcac cgagtacacg cttccgatcg
     1261 acgagcggct gcgcgccgcc gtccgcaacg accgcgcgcg gctgggccag atcgagatcg
     1321 aggtcgagag ccacctgcgg ccgcgggaca tccaggcccg gataagagcc ggtgcctccg
     1381 ccgaagaggt cgcgcagctg gccggcatcc ccgtcgaccg ggtgcgccgc ttcgagggcc
     1441 cggtgctggc cgagcgcgcc ttcatggccg agcgggcccg caagacgccg gtgcgccggc
     1501 ccggcgagaa caccggcccg cagctcggcg aggcggtcgc ggagcggctg ctgctgcgcg
     1561 gcgccgacaa ggacagcgtc cagtgggact cctggcgccg ggacgacggc acctgggagg
     1621 tgctgctggt ctaccgggtc gccaccgaac cgcactcggc gagctggacc tacgacccgc
     1681 cgcgccggct cgtccaggcc gtggacgacg aggcccgggc actgatcggc gaggccgacg
     1741 acacgcccga gccgagcttc ccgttcgtgc cgcggatcgc ccggctgccg cgcgaccggc
     1801 cgctggaccg cacgctggac cggcactccg agcgcggtga gcgcggtgcg caggccggcg
     1861 gcgaggacga cgcccggggc gcggaggagg ccaccggcga gcgggagcgg gattcgctga
     1921 ccagcctgct ggaggcggtg ccgagcttcc gcggcgacat ggtcgtaccg gagagcgcca
     1981 aggcgccgca ggccgacgag gagtccgagg ccgaggtcga ggagccgccg gccccggcgg
     2041 cgagcgcggg ggcgggttcc gcgtacgccg atgtgctgat gccgcgggca gtggccggcc
     2101 accgcgaccg gctgaccggg acgacggacc ggcaggccga ggcggacggc gtccggcccg
     2161 gccgccgggc cgcggtgccc agctgggacg agatcgtctt cggcacccgg cggaagaagc
     2221 cggagtagcg gcctcccgcc tcgtgacccc gtccgtccgc ccggtcccgc tcaggggccg
     2281 ggcggacggc cgcgtacggg gccgtgcagg gcccgtggcg gggctcagcc gggctgcggg
     2341 ccggtggcca ccggccgggc cgggtcggcg gaccattcgc tccaggaacc gacgtacagg
     2401 gccgcgggga tgcccgcgtg cgccagcgcc agcacctggt gggccgcgga gaccccggag
     2461 ccgcagtaga cgccgacctc ggcgtccggg gtggcgccca gcgccgcgaa ccgctcggcc
     2521 agctcggcgc ggtcccggaa gaccgtgccg ccctcggcga cgttctcggt cgtcggcgcg
     2581 gacaccgcac cggggatgtg gccggcgacc cggtcgagcg gctccacctc gccccggtag
     2641 cgctcgccgg cccgggcgtc cagcaggacg ccgcggcggg ccagggcggc cgcctcgtcc
     2701 gcggtcagca gcggcagcgc gcccggcacc ggcgtgaagt cgcccggttc gggagccggt
     2761 tgggcggtgc tcagctcgcc gcccgcggcc cgccaggcgg ccagcccgcc gtccagcacc
     2821 cgtacgtcct ggtggccgct ccagcgcagc agccaccagg cgcgggcggc cgcccagccc
     2881 tgtccgccgt cgtagacgac caccgggtgg tccgcgcgga ccccggcggc ctgcatcgcc
     2941 ttggcgaaga cgccgagatc ggggagcggg tgccggccgg ccgggccggg cggcgcggcc
     3001 agctcggcgt tgaggtcgac gtagaccgcg ccgggcacat ggccggcctc gtactcgggg
     3061 cggccgggcg ggcccccgag ctggtagcgg acgtccagga ccaccggagg agcggactgc
     3121 gccagctcgc tcgcgagttc ggtagcggtg atgatgacat tcatgggact catccttgcg
     3181 tagcggcgac tacgtacaga aggcgggtgc gcaccctccg tatgacggag ttacgctctg
     3241 cagtaacggc tccgaaacgg actcgaaaca tcaaaacggg cattctcctt tgcgaacgcg
     3301 ccgtggacgg cgcggccggg gcgcacacca ggacacaggg tgcgagcatc tgctcgggac
     3361 ccgcgcccgt acccccatgg tcagcccccg atcctccgag gagagtgacg atgacgaccg
     3421 aggcagcgac tcggcggatg cccggcgcgc cctgctgggt gagcctcctg gcccacagtc
     3481 ttaccgcgac ccaggagttc tacggcgcgc tgttcggctg ggagttccgc ccggggccgc
     3541 agcacttcgg tccgtacgcg cgggccttcc tcgacggccg ggaggtcgcc ggggtcggtg
     3601 agctggcgcc cgaccgtcat ctaccggtcg cctggaccac ctacctcgcc agcgacgacg
     3661 ccgatgtgac cgccgaacag gtccgttgct gcggcgggac ggtgggcgtc ggcccgctgg
     3721 acgcggacca tgacgcgggc cggatggcga tcgcctccga cccgcagggg gcgatcttcg
     3781 ggatctggca gggccggacg atgatgggca ccgcggtcac cggcgagccc ggcaccccgg
     3841 tgtggaacga gctggtcacc caggagaccg gcgccgtcag cccgttctac gagcacgtct
     3901 tcggtttcga ggccgagccg gctcccgccg acggcaccga ctccggctcc gactatctga
     3961 cgctgcgcct caagggccat ccggtggccg gcatccacgg cgtgggcaac gcaccgctcc
     4021 gcgaccgcgg cccccattgg atgacgtact tcgcggtccc cgacacggac gccgcggccc
     4081 gccgcgtcac cgaactgggc ggccacgtac tgcagccggc ccatgagtcc gcccacggcc
     4141 gggcggccac ggtcgccgac agcgagggcg cggtctttac gatcgttcag cagcgctgag
     4201 cgggcagcgc aaaaagtacc gcgcggtctt tttgcagtcg tcccgaatca ggagaccggc
     4261 tgcgcactcc tcgcctcgaa gagtcgctcg gcacggaacg gcgaggtgtc cagccgcgcc
     4321 agcaccccgg gaacggcgac gttgggcagg aagagctcca ggagccggga gatctcggcg
     4381 gagaggtcgg ggccgtgcgg cacggcctcc gcgacgagct gcacaccggt ccaggcaccg
     4441 acgaacagcc gtgccgtatc ggtcggatcg acgtgcggca gcagttcgcc ccgccgctgg
     4501 gcctcgatga gcagctcacg gcccagcacg atccagtcgg gccagcgggt accgaagaga
     4561 ctgcgcgcct tggagtcgac ggacaggcgt atcgaggcac tcaacagggg ctcccggggc
     4621 aggcggtagg ccagcaggag cgagatgtcc acccattcct ggagcttcag aacctgcggc
     4681 accaggccct ccgaggtcac cgcctcgtcg agcacaccgc gggcgagctc ctccttggag
     4741 gtgaagtgga agtacagcgc accacgggtg agcttggccc ggtcgatgat ggcggcgatg
     4801 gtggcggctt cgtagccgta ttcgttgaag atctccgcgg cggccaccag gcaggccttg
     4861 cgtgtgcgga tcgcccgttc ctgttttgcc aactcgcgtc tcccgtgacc acgtgatttc
     4921 cactgcattg aaaaagaccg cggacccggt attttacagc gcgtcacttc cttcacgcgt
     4981 gtcacttcta gggggaccgc atgcccgaag ccgccgcccg tatatgggag ttgccccaag
     5041 accgcgggtc tcccggcccc gtgctccgcg ttttcggtgc tcaggacagg gtggacgccg
     5101 ttgatccgac ggtagtgcct cacgagtacg cccatctcca ccacacggac cgggtgttca
     5161 tcactggctg gaaccgtcgc ggacccggcg cattctcgct caccgcccgg tggcctgccg
     5221 cccagggtga cgggcggtgc gatccgagag tgctggccca gaccttccgg cagagcgggc
     5281 tggtgatcgc ccacgcggaa tacggcgtac cgctctccca ccacatgctg ctgcacgacc
     5341 tgaacttcac ggccaatccg gatttccgcc tcccggaggg ccggtccacg gtgctcgacg
     5401 tcgaggtcac cgtcgcacag acccggcgca gcggccgttg tgccagcgct ctcggcatgg
     5461 tcgtccggat ccaccagggc gaggtcaccg tcgcacaggc cgagtcggag ttcgcctggt
     5521 cctcaccggc ggtgtaccgg cggctgcgcg gcgagcacct gggcgtcgac tggggtgcct
     5581 ggccacttcc cgcaccggtc gccccggagc tggtggggcg ggtcgacgag gcggacgtgg
     5641 tgctcgccgc cggagaccgc ccgcagcgct ggcagctgcg caacgacccc ggcaacacct
     5701 ggctgttcga ccaccccgtc gaccacgtcc ccggcctcgc cctcctcgaa gccgcctatc
     5761 aggccgccca cgccacggtc gcaccggccc acttcgcccc ggcaaccgtc accagcaact
     5821 acgagcgcta cgtcgagttc gacgaaccct gctggctcga aacggagtta cgccccgcca
     5881 ccccctcagg ccgcctcacc gtccttgtca ccggcaccca gggcggtcgg accgtcttcc
     5941 gcgcggggct gagcggtgcg gcctgccagg gctcgggggc gggacggctc actggttgac
     6001 caatccgccg tccaccggca gcacggtccc ggtcaggaac gggcagcggt cgctgagcag
     6061 ccatgccgcg gccgccgcga tctcctccgg agcggcggta cggccttgcg gcgtcgcgga
     6121 gttggccatc tcctccaggc cggggttgcg ggcgaaccag tcggtggtga tctcgctgcg
     6181 ggtggtgccg ggcgccaccg cgttcaccct gatgccctgg cgggcgtact cgtccgccgc
     6241 ggcccgggtg agccccacca cggcgtgctt ggaggcgatg tagggggcgg cggccgggat
     6301 cgcgatcaga ccggccacgc tgctgttgtt gacgatggtg ccggcgccct gcggaagcat
     6361 cgcggctatc tcgtggcgca ggcagttcca gacgccgcgc acattggtgt ccatgatggc
     6421 gtcgtacacg tcgtcggaca tcaggtggag cggggtgcgg tcgccgccga tgccggcgtt
     6481 gttgaaggcc gcgtccagcc gcccgtacgt cctgacggcg aagtccaccg cccgggcggc
     6541 gtcctcggcc acggacacgt cggcggcgac atacgcggcc tcggcgccct cggcccgcag
     6601 ggcgcccgcc agttcggcga gccgttcctc gcggcgggcg accaggacga ccgtcgcccc
     6661 ctcgtcggag aacacccgcg ccgccgcggc gccgatccca ctgctcgccc ccgtgaccag
     6721 gacgattttg ccggagagaa gtttgcccga gagaagatcg ctgttcatgg gacgagtctg
     6781 gcaggggccg ccccgggcga ggggtcagcc cgtcgcgtcc tgcggcgtcg gggcgaccgg
     6841 caggacgtcc ggggagagcg cgcccgcacg ggccgtggcg ctggtcatcc ggcgcaggtg
     6901 gtggcggcgg cagagcacct cgtagcccac ttccgcggtc gtctggcgga cgtcgccgac
     6961 cacgacctgg gcgccctcga cgaccatcgc gccgtccacc gtacgggcgt tgtgcgtggc
     7021 ccgggcgccg caccagcaca gcgcctcgac ctgcaacgcc tcaatgcggt cggcgagttc
     7081 gatcaggcgc tgggagccgg ggaacagccg ggtgcggaag tcggtggtga tgccgaacgc
     7141 gaagacgtcc aggtcgaggt cgtcgacgac gcgggccagc tggtcgatct ggccgggggc
     7201 gaggaactgg gcctcgtcca cgatcacgta gtccgcccgg ccgccacggg agaggtgcgc
     7261 gacgaggtag gcgtacaggt ccatgccctc ttcgacctcg acggcgtcgg tgaccaggcc
     7321 cagccgcgag gagagccggc cctcgccggc ccggtcgtca cgggtgaaga tgaccccctc
     7381 caggccccga gcggcgcggt tgtgcccgat ctgcagcgcc agggtgctct tcccgcaatc
     7441 catcgttccg gagaaaaata ccaattcgga catgggtcgg aggagggcct ttcgggtcgg
     7501 agtcgtcgcc cgcccaaggg agggcacggc ccgcgcgggg ccaacaggcg gagggcatgg
     7561 ggaagtgacg ggcggggtcg cgggcgggct gtggtcaggt gcggacttcc aggagcggca
     7621 ccagctgctc catggaggtc atcgacccgt gcatcccgac catctccgac tcccggggct
     7681 cccgttcggt cgcgatgatc accatgtcgg cgtgcgcggc cgcgaccacg tcgccgatcc
     7741 ggtcgcgcac ccggtcgtcg acgtacggcc cgaaccaccc ggcggcgatg gcctcttcgc
     7801 gactggcgac ccacatctgg tcgccgacca cctcgcgcca gacggcgagc acatcggcgg
     7861 ccgcgcccgg atgggcgtag acgtgccggg cccggccctc gccgcccaac aggcggacac
     7921 cggcgcggag ttcccagtcc tcgtcgaagt cgatccggga ctccgggccg aacgggatgt
     7981 cgatcatgcc gtggtcggcg gtgatgtaga gcgcgctgcg cggcggcagt tgctcggcca
     8041 gtcgctgggc cagccggtcg acgtacatca actgcccgcg ccaggcgtcg gagttgacgc
     8101 cgtagcggtg ccccttgccg tcgacctcgg agtagtacgt gtagaccagc gagcggtcgg
     8161 cggcgcccag ctgctcggcg gccaggtcca tccgctcctc gccggagagc cggccgtgga
     8221 aggagccgcc gctgagcgcg atcttggtga gcggggtgtg ctggaagtcc ggcgcggaga
     8281 cctggcaggt gtggatgccc gcggcgtccg cgagctggaa gaccgtcggg tacggctgcc
     8341 aggcgtacgg gtcggtccac gggtaccagc gcagctggtt catcagctca ccggtggccg
     8401 ggtcctggca ggtgtagccg ggcaggccgt ggacgccggg cggcaggccc gtgcccaccg
     8461 aggcgagcga ggtcgcggtg gtcgagggga agccggcggt gagcggacgg ccgctgccgt
     8521 tgaacgacgt ccccaggagg gaggtgagga acggtgcctc ctccgggtgc gcgcggagca
     8581 ggtcccagcc gaggccgtcg atcaggaaga cacaggcccg gtcggcgggc gcgaggtcca
     8641 tgccggtggc gagctcggtc atgccgaggc cggcggcgag ggtgggcagc agatcggcga
     8701 gcgagccggt gccgtactgc gggaccgggg cgcccagcgg gtcgagcggt tcgggttccg
     8761 gccaggcggg ggcgatgtcg accatcagcg gggggtggtg gtggccgcgg tggcctcgga
     8821 gagcgcctgg gcgaaggcga gcgtctcgcg caccgagtcg gggccgtcgc cggcctcgct
     8881 gacccgcagc gagaggtcgt cggcggtgga ggagccggtg tagccgtggt ccgcctcgca
     8941 gttggggtcg ccgcaggcgg cgggctccag gtccagccgg gccaccgcgc cccacccgat
     9001 ggtcaggacg acctcgcggg gcagctggcc gggggtgtac gactccgggt tggcgaccac
     9061 gcggctgagc accacggagg agatccggcc gagcttgacg gactcggtgg aggtggtggc
     9121 gtacggcgac ggggaggtgg cgtcggcggc ctgctcgtcg gtgtggctga cgatgaagcg
     9181 ggtgccggtc aggacgagga cggtgacgtg gcggcggacc tcgttggcgt cgaaggtcgt
     9241 ctcctggtgg acgaggtacg acaccaccgg ctcaccaccg accgcggcct gcaccgcctc
     9301 ggccacgagc gtcgggtaat agccactgcg ctcgatcgcc gcgcgcagcc cctgggtcgt
     9361 cgtaccggtc ttagccatgg ggtccatcct acggggctcg gcgggcacca cagcccccgt
     9421 ggggcggggt ccgtgcggtc cgggcggctc agtaggcggg caggcggcgg gggcccaggt
     9481 cggtggtggc gggcggccgg gccaggcgga cggcggcgtc caggacggac agaccgcgcg
     9541 gggcgacgac gaccggttcg aggtccaccc ccaccacctc ggggtggtcg tcgacgagcc
     9601 gggagacccg gagcagcagt tcggcgaggg cggcggtgtc caccggctgg gagccgcgcc
     9661 agccgaagag cagcggggcc gcgcggatcg agcggatctg ctcggcgacc tcacggtcgg
     9721 tggcggggat gagacggtgg gcgacgtcgc cgagcagctg ggagggggcg ccggccagac
     9781 cgaaggagag cacggcgccg gcggccgggt cgatggcggc gcggatgacc gtgtcgacac
     9841 cgcgcggcac catggactgg acgaccggcc ggagttcttc gggcgagccg aggaaatcgg
     9901 tcaattcggc gtagacgcgg cggagttcgg actcgccggc gatgtccagc cggactccgc
     9961 cgaggtcggc gcggtgccgg aggtggggcg cggtggtctt gagggccacc gggtagccga
    10021 ggcgggcggc ggcgcgcacg gcggcgtcgg cgtcgggggc gggcagcacg gggcgggtgt
    10081 ggatgccgta gcgggcgagc agggccgcgg cgtcgtcgtc cggcacccgc accgagcggc
    10141 cgtcggggac gtcggcgctg agccggtcga ggagccgctc gatgtcggca ccggccgcgg
    10201 cctcctggat gtcgtcgtac tcgggcaccc ggccggggtc ggccgcctcc cggcgccagg
    10261 cggcgtagcg gacggcctcg gcgagcgccc gtacggcccg ctcggccgcg gggtaggcgg
    10321 ggatgcgcag cggggcggcg cggcggcggg gcggggcggc ggcctcggcg gcgcggagcg
    10381 ggggtgtctg cgccgcggcg cgggctcccg ggggcgggcc cggcgggaac ggtggacggg
    10441 gcggcgggta cgggggtcgg ggtggcgggg cgggggctgc cgtcgtcggt gaggtggggg
    10501 cggccgtggc cggcgggtcg ggtgcggcgg gggccggcgg ggcgggccga tccgccgccg
    10561 gggagtcggg gccggggctg gggctgggcg gcggggcggc gagggtctcg gccagggcgt
    10621 ccatggcgag gtggacgacc gtgaccggct tctccgggtg ggccgctgcg gccgtgcgca
    10681 gggccgcggc gagcgcggcg ccctcgctca cgcccgggga ggcgccgtcc tcctccccca
    10741 cccaggggat ggccgtgacg atcaccgcgt cgcagccgtc gccggagagc gtctcgtcga
    10801 gcgcggcgcg gaagtcgtcc ggtgtcgcgg cggagggcag cacccggggg cgcagcggcc
    10861 ggaggcgctc ggtcagacag gcgtcgtagg cgagcagggc cagggactcg gagttgccga
    10921 ggatggcgat gcgcgggccg gtcggcagcg gctgggaggc cagcagcagc ccggcgtcga
    10981 tcagctcggt gacggtgtc
//
