LOCUS       NZ_AP026073            10984 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5574980
            Orig. end                         :: 5585964
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="19"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            986..1837
                     /locus_tag="HEK616_RS24950"
                     /old_locus_tag="HEK616_50930"
     CDS             986..1837
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536997.1"
                     /locus_tag="HEK616_RS24950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50930"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_261955177.1"
                     /transl_table=11
                     /translation="MTDQQTQRTPRSLIVTFYGAYGREEPGPADQSAGPVPVAALIRLL
                     GVLGVDPPSVRSAVSRLKRRGLLVPGRTVTGAAGYGLSDAGRQLLDDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAHLYEETRHTLERLEL
                     APYVDLFTGTHAGFAPTARSVARWWDLDVIAAQHRAFLAEQEPVLRRWSRRRTVPGEAA
                     YHDYLLALDAWRRLPYADPGLPSPLLPEDWPGGRAAEVFGRLHGTLQAAGARYVREVTA
                     GP"
     gene            complement(1907..2656)
                     /locus_tag="HEK616_RS24955"
                     /old_locus_tag="HEK616_50940"
     CDS             complement(1907..2656)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574418.1"
                     /locus_tag="HEK616_RS24955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50940"
                     /product="HAD family hydrolase"
                     /protein_id="WP_261955178.1"
                     /sec_met_domain="HAD_2 (E-value: 1.3e-27, bitscore: 95.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGARGTGVDTLRAAVFDLDGTLANTLPAISKLLVKVAAEQGCSV
                     TARQAAAAIGKPPGPAFGRLLGRPEDDGRVQAAINRYRELFSYEVLCQGPQLLFPGVSA
                     GLSALRAHGVELAVATSKPTRSAEALLDVMGIRDLVGPVIGQDMVRRGKPHPEMVLRAA
                     GRLGVAAWQTAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAAVAGTRMCSSFKDVV
                     SVIAAARPRPAMAAGAAGAARAVQRRR"
     misc_feature    complement(2851..2865)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.22"
                     /tool="antismash"
     misc_feature    complement(2877..2891)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            3140..3784
                     /locus_tag="HEK616_RS24960"
                     /old_locus_tag="HEK616_50950"
     CDS             3140..3784
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602522.1"
                     /locus_tag="HEK616_RS24960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50950"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_261955179.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQSLIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDSALATLQEIEDAFTRSPEPLPHAVRDFAVELFGRVRTDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGSPGGPAAADAPDAEPRRTAQLLT
                     SVVVGLLHLGGEDGTWWDEEAVAGLWALLPEAPDRVPAAGIPAPAQLPAAG"
     gene            4255..4872
                     /locus_tag="HEK616_RS24965"
                     /old_locus_tag="HEK616_50960"
     CDS             4255..4872
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="HEK616_RS24965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50960"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_261955180.1"
                     /transl_table=11
                     /translation="MVKQERALRTRRTVLEAAAHVIGHRGYQAATMAEIIQRAGVTKGA
                     VYFHFTSKDALARAVITEQAEPFLPQVSASRLQDAIDFTHQVSLALRSDPMLQAGTRIA
                     VETTFSEEPLMPYQVWTDIITTMFREARDNGELLPGVTPERAAEFFVAAYMGVQLFSRA
                     ATNRADLPERVTALWKHTLPGLAAPGALSQLDPHGRRTQIRI"
     misc_feature    4663..4665
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5984
                     /locus_tag="HEK616_RS24970"
                     /old_locus_tag="HEK616_50970"
     CDS             5001..5984
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="HEK616_RS24970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50970"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_261955181.1"
                     /sec_met_domain="AfsA (E-value: 2.9e-23, bitscore: 80.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLMPSSSPDQHHRQRRWVGGSRPSSSHTPVLGEYGHPTADS
                     EVFLTNWRRGSGDSWVVSAQWPRAHGFYAPAGGLHDPLLLVETVRQAGILLSHVAHRVP
                     LDHPIAWQHVRYALAPHALRTAGAPAEVELRITDHDVVRRGKRLISARQEFRIRCDGAH
                     LAHARLISSCHSPAVYRRLRGRYSDLAFATSRRLPVPEPVGARLVGRDHDRDVVLSPTD
                     LPDRWQLRVDTSHPVLFDHPVDHAPGMLMVEAARQAAQAVTPAVPTLPVALECSFERYA
                     ELDAPSWVRARTPEPATAGGARRVEVDVEQHGRPVAAARITSVPVP"
     misc_feature    5283..5285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6033..8495)
                     /locus_tag="HEK616_RS24975"
                     /old_locus_tag="HEK616_50980"
     CDS             complement(6033..8495)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602527.1"
                     /locus_tag="HEK616_RS24975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50980"
                     /product="oxidoreductase"
                     /protein_id="WP_410011249.1"
                     /transl_table=11
                     /translation="MAGEIAVRVAVIGGGPGGLYAAALLKRLDPTREITVFERNAPDDT
                     FGFGVVLSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRR
                     LLAVLHRRCRSLGVELRFRTEAPPAAELAREYDLVIAADGVHSATRTAHADVFRPQLTT
                     HRCRYIWLAADFAFDAFRFEIAETEHGVVQLHAYPYAAPAPAPAPAPAPAPAPAPAPAP
                     APAPAPAPAPAPAPASADTGPTPPHTRPAAESAGPASAPGASTVIVEMREEVWRAAGLD
                     RADERTSVEWCGKVFTDALRGRALRSNNSSWIAFRTVVNERWSHGNTVLLGDAAHTAHF
                     SIGSGTKLAVEDALALAACLQEQPDIPRALAAYEAERRPVVASTQRAARASLAWFEELA
                     GYVDQPPRQFAFNLLTRSRRVTHDNLRLRDPGFVAAVEDEAGVPAGTPPMFTPFRLRGL
                     TLRNRVVVSPMDMYSSEDGVPGDFHLVHLGARALGGAGLVMTEMVCVSENGRITPGCAG
                     LYTPEQAAAWRRVTDFVHTRAPGTALGVQLGHSGRKGSTKLMWDGIDEPLDTGNWPLVA
                     ASPLPYKPGSQRPHELTTGELADIRREFTDAARRAADCGFDLLELHCAHGYLLSGFLSP
                     LTNQRTDAYGGSLAGRLRFPLDVFDAVREVWPDDRPMTVRISATDWSDGGTTAEDAVAI
                     AGAFAEHGADAIDVSTGQVVSDERPDYGRSYQTPFADRIRNALGVPVIAVGAISSWDDV
                     NSLVLAGRTDLCALARPHLYDPHWTLHAAAEQGYTGPGAPWPLPYQAGSRTPPTGRTDA
                     PKPRLTLR"
     gene            complement(8480..9307)
                     /locus_tag="HEK616_RS24980"
                     /old_locus_tag="HEK616_50990"
     CDS             complement(8480..9307)
                     /NRPS_PKS="Domain: ECH (18-267). E-value: 2.8e-49. Score:
                     159.6. Matches aSDomain:
                     nrpspksdomains_HEK616_RS24980_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="HEK616_RS24980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50990"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_030811271.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTEAWQHIRVTREDGVATVTLARPEKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVV
                     RAVREAPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDAVRAPEAERLGLISELADEGRADEAAAALARRLAEGPALAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSADYAEFHAAFTEKRTPKWQGK"
     aSDomain        complement(8507..9253)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS24980_ECH.1"
                     /evalue="2.80E-49"
                     /label="HEK616_RS24980_ECH.1"
                     /locus_tag="HEK616_RS24980"
                     /protein_end="267"
                     /protein_start="18"
                     /score="159.6"
                     /tool="antismash"
                     /translation="VTREDGVATVTLARPEKLNALTFEAYADLRDLLAELSRERSVRAL
                     VLAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVVRAVREAPFPVIAAVHGVA
                     AGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGD
                     AVRAPEAERLGLISELADEGRADEAAAALARRLAEGPALAHAQTKALLTAELDMPLAAA
                     VEMDAATQALLMNSADYAEFHAAFTEK"
     misc_feature    9375..9384
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.04"
                     /tool="antismash"
     misc_feature    complement(9385..9405)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    9621..9636
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     gene            9913..>10164
                     /locus_tag="HEK616_RS24985"
                     /pseudo=""
     CDS             9913..>10164
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612071.1"
                     /locus_tag="HEK616_RS24985"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLKSPPSSNWRIALPRGATAVPIARAMVRCALQDLGTTADRTTAQ
                     LLTAELVANALKHTSGDHPIELVVERGPDGCQVEVHDGD"
     gene            <10492..10611
                     /locus_tag="HEK616_RS24990"
                     /pseudo=""
     CDS             <10492..10611
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005481794.1"
                     /locus_tag="HEK616_RS24990"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="RNRGLLLVRSLSSASGCRRTQDGKAVWFTLPEVHQPRRS"
ORIGIN
        1 gcgcaggtgc tgcggcccac cccggacgac gtgttcgcgg gcagcccgcc gctgggcttc
       61 accttcggcc tcggcggcct ggtcgtcttc ccgctgcggg ccggcgcctc ggccctgctc
      121 gccgactggg gcggccccga gcgactgctc ggcgacatcg cccggcaccg gatctcggtg
      181 ctgttcaccg cgccgaccgc ctaccgcgcg atgctgcccc ggctcgccgg acacgacgtc
      241 tcctcgctgc gccgctgcgt gtcggccggg gagaacctgc ccgccgccac ctggcagtcc
      301 tggtacgagg cgaccggcct gcggatcatc aacgggatcg gcgccacgga actgctgcac
      361 atcttcatcg cggccgccga cgaggcgatc cggcccggca cgacgggcct gccggtgccg
      421 ggcttcgagg cgcgggtggt ggacgcggac ggcgccccgg tgccggacgg cgagcccgga
      481 ctgctggcgg tccgcggccc gacgggctgc cgctacctcg cggacccgcg gcagaccgag
      541 tacgtaaggg acggctggaa cctcaccggc gacacctacg tccgcgagcc ggacggctac
      601 ttccgctatg tggcccgcgc ggacgacatg atcatctcgg ccgggtacaa catcgccggc
      661 cccgaggtgg aggacgcgct gctgcaccac cccgacgtga ccgaggcggc ggtggtcgga
      721 cggccggacg aggagcgcgg gcaggtggtg gtcgcccacg tcgtcctgcg ggccggcgtg
      781 ccccgggggc cggacaccgt cgccgcgctg cgcgccttca ccaagtcccg catcgccccg
      841 tacaaatgcc cgcgagagat cgtcttccac agcgcgctgc cgcgcacccc gaccggcaag
      901 ctccagcgct tccggctgcg cgcgccgcac ggggcggacg ccccggcccg cgggccggac
      961 ccggtcgccg atctacagtg atccggtgac cgaccagcag acacagcgca ccccacggtc
     1021 cctgatcgtc accttctacg gtgcgtacgg gcgcgaggag cccggcccgg cggaccagtc
     1081 ggccgggccg gtgccggtcg ccgcgctgat ccggctgctc ggcgtgctgg gcgtcgatcc
     1141 gccgtcggtg cgttccgcgg tctcccgcct caagcggcgc ggcctgctcg tccccgggcg
     1201 caccgtcacc ggggcggccg gttacgggct gtcggacgcc ggccgccagc tgctggacga
     1261 cggcgaccgg cggatcttcg gccggcccgc cgcgcggctg tccgacggct gggtgctggc
     1321 cgtcttctcc gtccccgagg aggaacggca caaacggcac ctgctgcgct cgcgactggc
     1381 ccggctgggc ttcggtaccg ccgccccggg tgtgtggatc gcccccgcgc acctctacga
     1441 ggagacccgg cacaccctgg agcggctcga actcgcaccg tacgtcgacc tgttcaccgg
     1501 tacgcacgcg ggtttcgcgc cgacggcccg gtcggtggcg cgctggtggg acctggacgt
     1561 catcgccgcg cagcaccgcg ccttcctggc ggagcaggag ccggtgctgc gccggtggtc
     1621 acggcggcgc acggtgccgg gcgaggctgc ctaccacgac tatctgctgg ccctggacgc
     1681 ctggcgccgt ctgccgtacg cggatcccgg actgccctct cccctcctgc cggaggactg
     1741 gccgggcggt cgcgccgcgg aggtcttcgg ccggctgcac ggcaccttgc aggcggcggg
     1801 cgcccgttac gtgcgtgagg tgacggccgg gccgtgaagg acacagggcc ggtgaagggc
     1861 cgtcagcgcg cctgaccacg cggcgcaccg tgcgccgtac cgtgcctcag cggcggcgct
     1921 gcacggcccg cgcggcgccc gcggccccgg cggccatggc gggacgcggc cgggcggcgg
     1981 cgatcaccga caccacgtcc ttgaacgaac tgcacatccg ggtgccggcg accgcggcca
     2041 actcgccgaa cgtgccgacc ccgtacgtca ccgcgaccgg ctccatccgc gcggtgaccg
     2101 ccatcctcat gtcccccacg gtgtccccca cgtacgcggt ctgccaggcc gcgaccccca
     2161 gccggccggc cgcccgcagc accatctccg ggtgcggctt gccgcgccgg accatgtcct
     2221 ggccgatgac cggcccgacc aggtcgcgga tgcccatgac gtcgagcagc gcctcggcgc
     2281 tgcgggtggg cttggacgtg gcgaccgcca gctcgacacc atgggcgcgc agtgcggaca
     2341 gtcccgcgct cacgccgggg aagagcagct gcggcccctg gcagagcacc tcgtaggaga
     2401 acagttcgcg gtagcggttg atggcggcct gcacccggcc gtcgtcctcg gggcggccca
     2461 gcagccggcc gaaggccggg ccgggcggct tgccgatggc cgccgccgcc tgccgcgcgg
     2521 tcaccgagca gccctgctcc gcagccacct tgaccaacag tttgctgatg gcgggcaagg
     2581 tgttggcgag ggtgccgtcc aggtcgaaga cggcggcccg gagcgtgtcg actccggtac
     2641 cccgtgcgcc tctcacggga cgttcgcggg cttccgttgc tacgcgcatg gctggagacc
     2701 tttcgggttc cggtgggggt gcgggttcgg gtgacgttgc cggctctgct ggtggggcgg
     2761 cccgcggtcg acgactcgtt acggttgagg cggtctcgcc atggcgcacc gctgtccaac
     2821 ggcggcacgc gaccgacggg gcggctgcaa cggaagggaa cgtcccgttt accgccttcg
     2881 gagatttaaa aaccgcctgc gctgtttgtc aatgcgcctt ggtcactccc tgacgggtcg
     2941 acaacgccct tcctggcgtt cagactgggg acacacgctt cacaggggcc gccgatgccc
     3001 cgaacgggcg gcggaccgtc gacttcccgt caggagacgc gagttccggc caaacccctg
     3061 aagcatatcg aaccgcatgg ttggttttct atgtagactg caatcagctc gcagccgtac
     3121 tagagacggg acggacaccg tgcccaccca gcatcgcgcc attcagagcc gccagtcact
     3181 catccgctcg gccgccgaga ccttcctgga gaagggggtc ccggccgcgg gcatggtcga
     3241 gatcagccgg cgggcccgcc tgagcaaggg cgccctctac ttccacttca cgtccaagga
     3301 cgacctgacc ctcgcggtgc gggactccgc gctcgccacc ctccaggaga tcgaggacgc
     3361 cttcacccgc tccccggagc cgctgccgca cgccgtacgg gacttcgccg tcgagctgtt
     3421 cggccgggtg cggaccgacg ccgtactgcg cgccggactg cggctgcggc ccgagaccgc
     3481 gccgggcctc ggcatctacg ccctggagca gcgctggtac gcgctcttcc tcgacaaggc
     3541 ggtcacctgc ggcaccggca gcccgggcgg accggccgcg gcggacgcgc cggacgccga
     3601 accgcggcgc accgcccagc tgctgacctc ggtcgtggtg gggctgctgc acctcggcgg
     3661 cgaggacggc acctggtggg acgaggaggc cgtcgccgga ctgtgggcgc tgctgccgga
     3721 ggccccggac cgggtgccgg ccgccggcat tcccgcaccg gcacaactgc ctgcggccgg
     3781 ctgaccccgg gccaccgcgc cgcaccagac cgccgcgcca caccgggccg cacccgcggc
     3841 ccgtcttcat acctccggcg aacgcacgcc acgccgatgc caccgggtgt ccgcgtcacc
     3901 gggtgtccgc gtgaacactc cacgaacacc gcgccgatgc cggcacgttc gataccgtcc
     3961 ggcgccagcg gccaccgccg tccccctcct cggcggtgaa ctcccgtcat ccgcaaggac
     4021 ggtggccagg tccaccgggc atgcagccgc cgtgacccgc gagccgccgc gcgtgcggcg
     4081 gcgggaccgg ccctcctccg cgaagtgccc cacgcccggc gggccttggg cacgggcggc
     4141 gcgcgggttc aataccgcat ggttggcttc tttatgatgg cgcaaataga ctgcgcggcc
     4201 accagcagcg gtcgaaggct caacaactcc aagagtgcat gcggaggatg atctgtggtc
     4261 aagcaggaac gcgcactgcg cacccgacgc acggtcctcg aagcagcggc tcatgttata
     4321 ggacatcgcg gttaccaggc ggccaccatg gccgagatca tccagcgtgc cggcgtcacc
     4381 aaaggggcgg tgtacttcca cttcacgtcc aaggacgcgc tggcccgtgc cgtcatcacg
     4441 gagcaagctg aaccgttcct cccgcaggtc agcgcgtccc gcctgcagga cgccatcgac
     4501 ttcacgcacc aggtgtccct ggcgctgcgc agcgacccga tgctgcaggc cggcacccgg
     4561 atcgcggtgg agacgacctt cagcgaggaa ccgctgatgc cgtatcaggt gtggacggac
     4621 atcatcacga cgatgttccg cgaggcccgg gacaacgggg aattactgcc cggcgtgact
     4681 cccgaacggg cggccgagtt cttcgtcgcc gcctacatgg gcgtgcagtt gttctcgcgg
     4741 gccgcgacca atcgcgccga cctccccgag cgggtcacgg cgctgtggaa gcacaccctg
     4801 ccggggctgg ccgcgcccgg cgcgctgagc cagctcgatc cgcacggtcg ccggacgcag
     4861 atccgcatct gatccgtgcc gcactccgac atgtggcccc tggcaaacaa accacatggt
     4921 ccgtatctta gttcctctgg gccgtcgtcg gtcgttcgcc cgtgagggcg ctgagcgccg
     4981 caccgcagaa agggacgcct atggccagcg ccaccctgat gccctcctcc tcgcccgatc
     5041 aacaccaccg gcagcgccgg tgggtgggag gaagccgacc gtcctcgtcg cacaccccgg
     5101 ttctcggcga gtacggacac cccaccgccg actccgaggt gttcctcacc aactggcggc
     5161 gcggttccgg cgacagctgg gtggtgagcg cccagtggcc gcgggcccac ggcttctacg
     5221 caccggccgg cgggctgcac gatccgctgc tgctggtcga gaccgtacgg caggcgggga
     5281 tattactgag ccatgtcgca caccgggtgc cgctcgacca ccccatcgcc tggcagcacg
     5341 tccgctacgc cctcgccccg cacgcactgc gcaccgccgg cgcaccggcc gaggtggagc
     5401 tgcggatcac cgaccatgac gtggtgcgcc gcgggaagcg tctgatcagc gcccgccagg
     5461 agttccgcat ccggtgcgac ggcgcccact tggcccatgc ccggctcatc tcctcctgtc
     5521 acagcccggc ggtctaccgc cggctgcgcg gcagatacag cgacctggcg ttcgccacct
     5581 cccgtcggct gccggtgccg gagccggtcg gcgcgcggct ggtcggccgg gaccacgacc
     5641 gcgatgtggt gctctccccc accgacctcc cggaccgctg gcaactgcgg gtcgacacct
     5701 cccatccggt gctcttcgac catcccgtcg accacgcccc cggcatgctg atggtcgagg
     5761 ccgcccggca ggccgctcag gcggtgacgc ccgccgtgcc gacgctgccg gtcgcgctgg
     5821 aatgctcctt cgagcggtac gccgagctgg acgcgccgag ttgggtgcgg gcgcgcaccc
     5881 cggaaccggc cacggccggc ggcgcgcggc gggtcgaggt cgacgtggaa cagcacggaa
     5941 gaccggtggc cgccgcgcgg atcaccagcg tgccggtgcc ctgagggtcc ggctccccag
     6001 cggccccgcc cgcgctgccc gtgcctcccc cgtcaccgca gcgtgaggcg gggcttcggt
     6061 gcgtcggtgc gcccggtggg cggggtgcgg ctgcctgcct ggtagggcag cggccagggg
     6121 gcgcccgggc cggtgtagcc ctgctcggcg gcggcgtgca gggtccagtg cgggtcgtac
     6181 aggtgcggtc gggccagcgc gcacaggtcg gtgcgcccgg cgaggacgag ggagttcacg
     6241 tcgtcccagg aggagatcgc gccgaccgcg atgaccggca cgccgagggc gttgcggatc
     6301 cggtcggcga acggcgtctg gtacgagcgt ccgtagtcgg ggcgctcgtc ggagacgacc
     6361 tgcccggtgg agacgtcgat ggcgtcggcg ccgtgctcgg cgaacgcgcc cgcgatggcc
     6421 accgcgtcct cggcggtggt gccgccgtcg gaccagtcgg tggcggagat ccggacggtc
     6481 atcggccggt cgtccggcca cacctcgcgc accgcgtcga agacgtcgag cgggaagcgg
     6541 agccggccgg cgagggaacc tccgtaggcg tcggtgcgtt gattggtcag cggggagagg
     6601 aatccggaca gcagatagcc gtgggcgcag tgcagttcga ggaggtcgaa gccgcagtcg
     6661 gcggcccgtc gcgcggcgtc ggtgaactcc cgccggatgt cggcgagttc gccggtggtc
     6721 agctcgtggg ggcgctggct gccgggcttg tagggaaggg gggatgcggc gacgagtggc
     6781 cagttgccgg tgtcgagcgg ttcgtcgata ccgtcccaca tcagcttggt ggagcccttg
     6841 cggccggagt ggccgagctg cacgccgagc gccgtgccgg gcgcccgggt gtgcacgaag
     6901 tcggtgacgc ggcgccaggc cgccgcctgt tcgggcgtgt agagaccggc gcagcccggg
     6961 gtgatccggc cgttttcgct gacgcacacc atctcggtca tcacgagccc ggccccgccg
     7021 agggcccggg cgccgaggtg tacgaggtgg aagtcaccgg ggacgccgtc ctcggaggag
     7081 tacatgtcca tcggtgacac caccacgcgg ttgcgcaggg tgagtccgcg cagccggaag
     7141 ggcgtgaaca tcggtggggt gccggccggg acgcccgcct cgtcctcgac ggcggcgacg
     7201 aacccggggt cgcgcagccg caggttgtcg tgggtgacgc ggcggctgcg ggtgagcagg
     7261 ttgaaggcga actggcgcgg cggctggtcg acgtacccgg cgagctcctc gaaccacgcg
     7321 aggctggcgc gggcggcccg ctgggtggag gcgacgacgg gccggcgctc cgcctcgtag
     7381 gcggcgaggg cccgggggat gtcgggctgc tcctggaggc aggcggccag ggccagggcg
     7441 tcctcgacgg cgagtttggt gccggagccg atggagaagt gcgcggtgtg cgcggcgtcg
     7501 ccgagcagca cggtgttgcc gtgcgaccag cgctcgttga cgacggtgcg gaaggcgatc
     7561 cagctggagt tgttggaccg cagggcccgg ccgcgcaggg cgtcggtgaa gaccttgccg
     7621 caccactcca ccgacgtgcg ctcatccgcc cggtccagcc cggcggcccg ccagacctcc
     7681 tcccgcatct cgacgatgac ggtgctggca ccgggggcgg atgcggggcc ggcagactcc
     7741 gccgcagggc gggtgtgggg cggggtgggg ccggtgtcgg cggaagcggg agcgggagcg
     7801 ggagcgggag cgggagcggg agcgggagcg ggagcgggag cgggagcggg agcgggagcg
     7861 ggagcgggag cgggagcggg agcgggagcg ggagcggcgt agggataggc gtggagttgg
     7921 acgacgccgt gctcggtctc ggcgatctcg aagcggaagg cgtcgaaggc gaagtccgcg
     7981 gcgagccaga tgtagcggca gcggtgggtg gtcagctgcg gccggaagac gtcggcgtgc
     8041 gcggtgcggg tcgcgctgtg caccccgtcg gcggcgatga ccaggtcgta ctcgcgggcc
     8101 agttcggcgg cgggcggcgc ctcggtacgg aagcgcagct ccacgccgag gctgcggcag
     8161 cgccggtgca gtacggccag cagccggcgc cggccgagtg cggcgaagcc gtgcccgccg
     8221 gaggtgaggg tgcggccgcg gtgcacgatg tcgatgtcgt cccagcggac gaactccgcc
     8281 tggagcgcgc ggtagacctc cgggtcggcg tgctcgatgc cgccgagcgt ctcgtcggag
     8341 aggacgacgc cgaagccgaa ggtgtcgtcg ggggcgttgc gctcgaacac ggtgatctcg
     8401 cgagtggggt cgagccgctt gaggagcgcc gcggcgtaca gtccgcccgg tccgccgccg
     8461 atgaccgcga cccgcacggc tatttcccct gccacttggg cgtccgcttc tcggtgaagg
     8521 cggcgtggaa ttccgcgtag tcggcgctgt tcatcagcag tgcctgggtc gcggcgtcca
     8581 tctcgacggc ggcggcgagc ggcatgtcga gttcggcggt gagcagcgcc ttggtctgtg
     8641 cgtgcgccag cgcgggcccc tcggcgagcc gccgggcgag cgccgcggcc gcctcgtcgg
     8701 cccggccctc gtcggccaac tcgctgatga ggccgagccg ttcggcttcc ggtgcccgga
     8761 ccgcgtcgcc gagcatcagc agccgggtgg cgtgtccgag gccgatgacc cgcggcagca
     8821 gataggccgc gcccatgtca ccgccggaca gcccgacccg ggtgaagagg aacgcgaagc
     8881 gggccgaggg gtcggcgacc cggaagtccg cggccagtgc gagcaccgct ccggcaccgg
     8941 ccgccacgcc gtgcaccgcc gcgatgaccg ggaacggggc ctcccgcacg gcccgcacga
     9001 cctgcccggt catccggttg aagtccagca gctggccggt gtccatgccc agggtggcgc
     9061 cgatgatctc ctcgacgtca ccccccgagc agaacccgcg cccctccccc gccagcacga
     9121 gggcgcgtac cgagcgctcc cgggacagct cggcgagcag atcacggaga tcggcgtacg
     9181 cctcgaaggt gagcgcattg agcttctccg gtcgcgccag ggtgaccgtg gcgaccccgt
     9241 cctccctggt cacacggatg tgctgccagg cctcggtact acgggcggat cctgcgaacg
     9301 gactcatcgg actgcctgcc cccttcggcg ggtcggccgg tggtgctctt ccggatgccc
     9361 ttcgaagcta tcactgttct gtgactgtcg tcatgagggc gcgatactgg gaaatggtgc
     9421 ggccgtcggg aatgccgcgc ccctcgggga caccacgccc gtcggagatg ccgcgcccgc
     9481 cggaaatgcc gagcccctcg ggaatgccgc gtcccgccct cgcgttgtcg gccccgaggg
     9541 ggcgcgcgcg gccactaggg gatctgttcc cctgccccct aaggcccgcc cccgaccacc
     9601 cctgatcgga ccagcgggac ggcaccaccg ggtcacccct cgcccgccgc ctgctggtca
     9661 ccgatcgccc gcacgggccc gtacgcccgc cccgccccca ttacccgtac ggcccgtacg
     9721 cccgcgccac ccccgtacga gcccgagacc ggtccgccgg gtcgaaggtc ccgcccgttc
     9781 gggactttcg tcctgcgtcg cgtatgacct gcgtcacttg ccttgcggcc gcccggccct
     9841 gccccggcca cccccgcgct tcctacggtg gaaccgtcca agccggcccc tcactaacgg
     9901 aacgtcgcgc gcatgctgaa atcgcctccg tcgtcgaact ggcgcatcgc gcttccccgg
     9961 ggagcgacgg ccgtccccat cgcccgtgcg atggtccgct gcgccctcca ggacctgggg
    10021 accacggcgg accgcaccac cgctcagctg ctgaccgccg agttggtcgc caacgcactc
    10081 aagcacacca gtggcgatca ccccatcgag ctggtggtgg agcgggggcc ggacgggtgc
    10141 caggtggagg tgcacgacgg cgacacggtg ctcgtcgagg ggctgggaga gccgatgccg
    10201 atggcgatgc cgatgccgat gccgatggcg atgccggcgg tcggaccggg cgagccgctg
    10261 ttgatgaccg ggccgggcgc gaccggatcg ggcacaccag tcccgttggc cgaaccgggg
    10321 gacgtaccgc tcgcaacgcc ccccgcagcg gaaacggaac cggacgtgga accggacgtg
    10381 gaaggggaag cagaagcgac gggacccgac gcagcccccg ccactgcggc cgtcgctcca
    10441 gccgccccca ccgttccggt agccgcaacc gcaccccccg cccccgccat ccggaaccgc
    10501 gggctgctcc tggtgcgttc cctcagctcc gcctccggct gtcggcgtac ccaggacggt
    10561 aaggcggtgt ggttcaccct tcccgaagtg caccagccgc gccggagctg accgccgccc
    10621 cacgctcctt ctcgtcgtcc ctctcgacgt ccttcgcgtc ggccttctcg tcgtccttcg
    10681 cgtcggcctt cgcgtcggcc ttcgcgccgt tcttctcgtc gcccccgtgg ccgtcccgct
    10741 cgccgtccgc ggtcggcgcg gagaccgctt cgagcccggc gccctggccg aggcgggaat
    10801 ggcgacgtcc gtagagcgcg tagaccagcg cgccggccac caggaacgcc acgaactgca
    10861 gccaggtcgc cggtccggtc ccgtagatca ggtagccgca gaacgcgatg ccgaggatcg
    10921 ggctgagcgg gaagaacggg acgcggaagc ggcgcggcag gtcgggccgg gttcggcgga
    10981 gcac
//
