LOCUS       NZ_AP026073            11378 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5516865
            Orig. end                         :: 5528243
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11378
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11378
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11378
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6378](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6378
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..79)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(1215..1385)
                     /locus_tag="HEK616_RS24720"
                     /old_locus_tag="HEK616_50440"
     CDS             complement(1215..1385)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133761.1"
                     /locus_tag="HEK616_RS24720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50440"
                     /product="hypothetical protein"
                     /protein_id="WP_261955134.1"
                     /transl_table=11
                     /translation="MPHPPAAPDPAAPAPDAPVADCLHCGRPTAYPVDRPGIVLCPVCE
                     WQEAQRTACSG"
     gene            complement(1438..2106)
                     /locus_tag="HEK616_RS24725"
                     /old_locus_tag="HEK616_50450"
     CDS             complement(1438..2106)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088894.1"
                     /locus_tag="HEK616_RS24725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50450"
                     /product="ABC transporter"
                     /protein_id="WP_261955135.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLVGSHRWLPPVILYAAFLAIGIQSGGPILDAFGY
                     AAVGVLPVTVWLTRICVANEPPAARSCTAAAAGPGRVHLASVLTAAGAALLLGLLGTAL
                     VVLLSDPHSAKGRVAVPVLPATGAGLCAVLACLLTGLAIGALCTWPVLRVPGWGVPAGL
                     LGSLLLLVLGGSPANAAMTGLVTGSLHGTITVPWLPAAAAALLAAAATAAACALAPRRS
                     "
     gene            complement(2103..3059)
                     /locus_tag="HEK616_RS24730"
                     /old_locus_tag="HEK616_50460"
     CDS             complement(2103..3059)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="HEK616_RS24730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50460"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_261955136.1"
                     /transl_table=11
                     /translation="MPERMRLDGVGRRYGVRGPWVLREVHLTVPEGALLRIEGTNGTGK
                     STLLRLLAGIDRPSTGRITGRPRRAYVPERFPAAMPFTAVGYLTHLGRVHGLTGAAAAR
                     RAAEWLERFGAAGHARTRLAELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDRGAREV
                     LDRAVAERVADGGTVVFVDHDPQRLAGAADARYRVTDGRLLAQEDRPGTPADPGAATGP
                     RVTVEAEGPPGAAPPTGPPGTPPGAPRLPGGPEITPGPDGAFRLTVPAVHSDALLRALL
                     TARPPWHIRAVTAAPGPAAPPAAANPPAPDPDEAPLP"
     gene            3421..4173
                     /locus_tag="HEK616_RS24735"
                     /old_locus_tag="HEK616_50470"
     CDS             3421..4173
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602443.1"
                     /locus_tag="HEK616_RS24735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50470"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_261955137.1"
                     /transl_table=11
                     /translation="MTRTETAGQSGNDSTSPFRPDESDRDAALQFVLPLVVRLERADPP
                     ARTDALETAARAVLVLLSDERSAGDGEWAAAVRDWQDARIRKVVRRARGAEWRRAEALP
                     GITVTGRRAEVRVYPPVLLDGWPKDLVKLQVSGTELDDPEPPGPPADGTPVLWLNPALE
                     MSAGKEMAQAGHGAQLAWWELSEAERAAWRAAGFPLAVRSADPARWEALTRSGLPVVRD
                     AGFTEIAPGSCTVVADHPALRDALSRGR"
     gene            4181..4855
                     /locus_tag="HEK616_RS24740"
                     /old_locus_tag="HEK616_50480"
     CDS             4181..4855
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="HEK616_RS24740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50480"
                     /product="LysE family translocator"
                     /protein_id="WP_261955138.1"
                     /transl_table=11
                     /translation="MLTSLLGFTLVAAVLTVSPGPDFAVVLRTALGSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLAASTTAYDVLRVAGALYLMWLGIQALRSARRLRTDGAQEA
                     GAAPAAARGRSRDGEGPRPATPLRALRTGLLTNVLNPKVGVVYMSLLPQFIPHGAPVLA
                     TTLLLVAVHAALGVLWLGGIAVAVHRARAVFQRPRIRRRLDQATGGVLLALGAAVGFET
                     AR"
     gene            5001..6378
                     /locus_tag="HEK616_RS24745"
                     /pseudo=""
     CDS             5001..6378
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983658.1"
                     /locus_tag="HEK616_RS24745"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 6.1e-96, bitscore: 319.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRQGTDIERLGTGIGWRPEIAAEIAGLPGVDWVEVVAENICPGH
                     LPVALRELRERGTTVVPHGVSLGLGGADRPDAGRLAALAERAEVLGAPLVTEHIAFVRA
                     GGPLTASPLMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALVNWPDEEL
                     TEGQFLAELVERTGVRLLIDVANLHTNHVNRGEDPAVALDELPVEAIAYVHVAGGVERD
                     GVWHDSHAHPVTRPVLDVLAELCARITPPGVLLERDDDFPEAGELAGELDAIRTVLKEA
                     RHVQAA"
     gene            6378..7421
                     /locus_tag="HEK616_RS24750"
                     /old_locus_tag="HEK616_50510"
     CDS             6378..7421
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055541.1"
                     /locus_tag="HEK616_RS24750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50510"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_261955139.1"
                     /transl_table=11
                     /translation="MSFTQAVYVGLGASSLVLLLGTVLVRRGTPSVRRGEAQARNVWEM
                     AFLAGGPGRVVDAALAGMCEDGRLAVGGPGVVTVRQATAQHAVESAVLDAVARTPGGAL
                     AAVRAEAMRSPAVQGVGDRLAARGLMRHPRRGQGWRQFAGAQRAVCVALFVLGVLLSIA
                     DAGPDDGVDEGFGPPVVITIMPALIGGLIVASLCRRVFRNRVTKAGALAVRSARATHAQ
                     GGTGDAWQAAGLVVALGGTALIADELLRQQFEEAQRATALATAGGSSSSGSASSGYDSG
                     GGAGGDSSFWCGSSDGGSGSSCGSGSSCGGSSCGSGSSCGGGSSCGGGSSCGGGSSCGG
                     GSGCGSS"
     misc_feature    complement(7583..7598)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.08"
                     /tool="antismash"
     misc_feature    complement(7588..7603)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.08"
                     /tool="antismash"
     misc_feature    complement(7591..7608)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    complement(7605..7620)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.09"
                     /tool="antismash"
     misc_feature    complement(7632..7645)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.75"
                     /tool="antismash"
     gene            7651..8469
                     /locus_tag="HEK616_RS24755"
                     /old_locus_tag="HEK616_50520"
     CDS             7651..8469
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="HEK616_RS24755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50520"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_261955140.1"
                     /transl_table=11
                     /translation="MWLLFLLVAAVAAVFSCARVCRAAVAAARTGRPARPPAPAPAPDP
                     AGDDGHAPGLTLYEMAYLSGGPQRLADVVLVLMAQKRRLLLAHTGWATVVEPVAQDPVE
                     RATLSAIGPDGQRPIPAVREALVADEAVRAVADKLVADGLALPAAARPGIRSGIRQVWA
                     AALLVVLTAAAAYWMAPPGTDTALLLGWFSLPLVLCLGTLAVARFELHPYRDWATDEGE
                     QRLPGKADPPGSRLPSAPLAPLTSLISLALHGPSALTDPALRAALHSSGA"
     gene            8554..10239
                     /locus_tag="HEK616_RS24760"
                     /old_locus_tag="HEK616_50530"
     CDS             8554..10239
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (93-507). E-value:
                     5.1e-18. Score: 57.5. Matches aSDomain:
                     nrpspksdomains_HEK616_RS24760_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="HEK616_RS24760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50530"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_261955141.1"
                     /sec_met_domain="PF00561 (E-value: 7.6e-21, bitscore: 73.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLIVTALATAPAGGTTQAAGAPPAPVAHRIDFGR
                     CAPVEHLPSSVECGKLTVPLDYARPDGKKIQLTVSRARATAHRERQGSLVFNPGGPGAS
                     SLEFPLYGALPKWSRLARAYDFVGYAPRGVGRSAPLSCQDPKEFAKAPTDSRMYPDAAF
                     KRKKRAQAMAYARGCARRAGADLPFYNSVNNARDLDMLRAALGEKKLTFIGASYGTYFG
                     AVYATLYPGHVRRMIFDSVVNPEPRQIWYRNNLDQDIAFERRWGDWLGWVAKHDDHYHL
                     GTTKKAVQQAYDTVAGKLRRHPVDGKIGAGELQSAFLKTGYNDGFWPLRAEALASYLHG
                     DPKPLIAQALPQQTGAKDAENANAVYTAVECNDAPWPRSWRTWDRDNTDVAEIAPFETW
                     DNAWMNLPCAFWPERTQSAAAGVEAAARRVGQGSGQELADAAGHAAATELDGALHGRRL
                     PLDVHTEPGALPPVLLLAAERDAATPYRGALELQRRLPGSSLVTENGAGTHGIGFGEND
                     CVNRYAEAYLLHGKIPAHKVYCAPRAEPVPGEQTTRAQHARPGK"
     aSDomain        8833..10074
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS24760_Abhydrolase_1.1"
                     /evalue="5.10E-18"
                     /label="HEK616_RS24760_Abhydrolase_1.1"
                     /locus_tag="HEK616_RS24760"
                     /protein_end="507"
                     /protein_start="93"
                     /score="57.5"
                     /tool="antismash"
                     /translation="LVFNPGGPGASSLEFPLYGALPKWSRLARAYDFVGYAPRGVGRSA
                     PLSCQDPKEFAKAPTDSRMYPDAAFKRKKRAQAMAYARGCARRAGADLPFYNSVNNARD
                     LDMLRAALGEKKLTFIGASYGTYFGAVYATLYPGHVRRMIFDSVVNPEPRQIWYRNNLD
                     QDIAFERRWGDWLGWVAKHDDHYHLGTTKKAVQQAYDTVAGKLRRHPVDGKIGAGELQS
                     AFLKTGYNDGFWPLRAEALASYLHGDPKPLIAQALPQQTGAKDAENANAVYTAVECNDA
                     PWPRSWRTWDRDNTDVAEIAPFETWDNAWMNLPCAFWPERTQSAAAGVEAAARRVGQGS
                     GQELADAAGHAAATELDGALHGRRLPLDVHTEPGALPPVLLLAAERDAATPYRGALELQ
                     RRLPGSSLVTENGAG"
     gene            complement(10304..11056)
                     /gene="hemQ"
                     /locus_tag="HEK616_RS24765"
                     /old_locus_tag="HEK616_50540"
     CDS             complement(10304..11056)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="HEK616_RS24765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_50540"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_261955142.1"
                     /transl_table=11
                     /translation="MTDASTPASAPQNPAEPDKAPNAGKKAKDLNEVIRYTLWSVFKLR
                     DVLPEDRTGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADVMIWWHSESSDALQDAY
                     NLFRRTRLGRALDPVWSNMALHRPAEFNKSHIPAFLADERPRAYVSVYPFVRSYEWYLL
                     PDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGS
                     EARMHVREEVPFYTGRRKAVAELVAGLA"
ORIGIN
        1 ggtcctctcc cgggagggcg agccgctgcg ctccggcgag ggcagggtgc ccggcaactg
       61 cgacggcatg gccgccttcc ccgcccccgg ccggcgcggc gggacctgcc tcgtccgcaa
      121 ccacgagaac cgtacgggct cccgctccgc cgtcccccgg gtgcgcgagc tgacctacga
      181 ccccggtggc gaggggggct gtaccgccct ggaactggac cgcgacaaca cggtgcgctc
      241 ggaacgggtc gccatcgccg gcacctccac caactgcgcg ggcggcccca ccccttggca
      301 cacctggctg acctgcgagg agaccgagtt ccgggccggc gacgagaact acaccaagga
      361 ccacggcttc atcttcgagg tggacccgta cgacccgcgg cgcaccgggg cggtaccgct
      421 gaccgcgatg ggccgcttcc agcacgaggc gatcgccgtg gaccccggca ccggcaccgt
      481 ctacgagacc gaggacgcct tcgagaagcc cttcgggctc ttctaccgct tcctgcccga
      541 caagccgctg ggcggccggg gttcgctccg ggccggcgga cgcctccagg cgctccgggt
      601 gcccggcgtg cccgacctgt ccaccgtcca ggcgcccggc accgtcttcg accacgtcag
      661 ctgggtggac gtgcccgacc cgctggcccg gaccacgccg atccgcttcc aggacttcgg
      721 taccggcggc atcacccacg cgcagaaact ggagggctgc tactggggcg gttcctgcgt
      781 ccacttcgtc tccagcttcg cgcaccgctc cgccggttcg gcggccgacc actacggcca
      841 ggtctggcgg tacgacccgc ggcggcgccg gctcaccctc gtcgtcgtct tcggcccgga
      901 caccgacctc caactgcccg gcgagtcccc cgacaacatc tgcctggcgc ccagcggggg
      961 cctgatggtc tgcgaggacg gcgacggtgc ccagcacgtc ttcgggctca cccggggcgg
     1021 cgaggtctac ccgatggccc gtgggcggca gaacatcggc acgccccaga agccggagtg
     1081 gggcgagttc gcgggcgtga ccttcgcgcc cgaccaccgc acgatgtacg tcaactgcta
     1141 caggccgggc acgacgttcg cggtgaccgg gccctggcac cactgacgcc gccgcggccg
     1201 cgccgcccca cggatcagcc ggagcacgcg gtccgctgcg cctcctgcca ctcgcacacc
     1261 gggcagagca cgatccccgg ccggtcgacc gggtacgcgg tgggccggcc gcagtgcagg
     1321 cagtcggcta cgggggcgtc gggggccggg gcagcgggat cgggagcggc gggcgggtgc
     1381 ggcatggggc cgagggtacg cccaccggtc ccggggcggc ccgccctccc ccgtggttca
     1441 gctgcgacgg ggcgccaggg cgcaggcggc ggcggtggcg gcggccgcga gcagggcggc
     1501 cgcggcggcc gggagccagg ggacggtgat cgtgccgtgc agggagccgg tgaccaggcc
     1561 cgtcatggcg gcgttggcgg gcgagccgcc caggacgagc agcagcagcg agccgaggag
     1621 cccggccgga acgccccagc ccgggacgcg cagcaccggc caggtgcaca gcgcgccgat
     1681 cgccaggccc gtcagcagac aggccagcac ggcgcacagg ccggcgccgg tcgcgggcag
     1741 gacgggtacc gccacccggc ccttggcgga gtgcggatcg gacagcagca ccacaagggc
     1801 cgtaccgagc agcccgagca gcagcgcggc accggccgcg gtcagcacgg aggcgaggtg
     1861 cacgcggccg ggcccggcgg cggccgccgt gcaactgcgg gcggcgggcg gctcgttggc
     1921 gacgcagatc cgggtcagcc agacggtgac cggcagcaca ccgaccgcgg cgtagccgaa
     1981 cgcgtcgagg atcgggccgc cgctctggat gccgatggcc aggaacgccg cgtagaggat
     2041 gaccggcggc agccagcggt gcgagccgac gagcagcgcc gcctgatagc ggaggagagc
     2101 ggtcacggga ggggggcctc gtcggggtcg ggggcgggcg ggtttgccgc agccggcggt
     2161 gcggccgggc cgggggcggc ggtgacggcg cggatgtgcc acggcggacg ggcggtgagc
     2221 agggcgcgca gcagcgcgtc ggagtggacg gcggggaccg tgagccggaa cgcgccgtcc
     2281 ggaccgggag tgatctccgg gccgcccggt agccgcgggg ctccgggggg tgtcccgggc
     2341 ggcccggtcg gcggggcggc gcccggtggg ccctcggcct cgacggtcac ccgggggccg
     2401 gtcgcggcgc ccggatccgc gggcgtaccg ggccggtcct cctgggccag cagccgcccg
     2461 tccgtgaccc ggtagcgggc gtcggccgcg ccggccaaac gctgcgggtc gtggtcgacg
     2521 aacaccaccg tgccgccgtc cgcgacccgt tcggcgaccg cccggtcgag cacctcacgg
     2581 gcgccgcggt ccaggccggt ccacgcctcg tccaggacga ggagttcggg ctcggcgagc
     2641 agcgcctggg cgaccgcgac cttctggctg gtccccttgg acagctcggc cagccgggta
     2701 cgggcgtgcc cggccgcgcc gaagcgttcc agccactcgg cggcccgccg ggccgctgcc
     2761 gcgccggtca ggccgtggac gcggccgagg tgggtgaggt agccgacggc cgtgaagggc
     2821 atggcggccg ggaagcgttc ggggacgtag gcgcggcgcg gccggccggt gatgcgtccg
     2881 gtgctgggcc ggtcgatccc ggcgagcagc cgcaggaggg tggacttccc ggtgccgttg
     2941 gtcccctcga tgcgcagcaa cgcgccttcg ggcaccgtga ggtggacctc gcgcagcacc
     3001 caggggccgc ggaccccgta gcgccgcccc accccgtcca gccgcatccg ctccggcatc
     3061 cgctgcctcc ccgtgtcccc gcgctgcccg gcccggcatc cgatcgcccg tgccggcccg
     3121 cccgttggcg tacggcgtcg gtccaccgcc gtacggcatc ggtcccctgc cgtaccgcgt
     3181 cggtccgccc gccgtccccg acggtagcgg gaccggccgc cgggcgcccg ctcggcgacg
     3241 cggcgggcga cgcggtgcgg gcgggctggt ccgcgcgggc gagcgggctg gtccgcgcgg
     3301 gcgggcctgc gcgcgaaggg ggcagcatgg gcgggcccgc ggcggcgcgt caggggtgcg
     3361 cgggcgggcc tgcgcgggcc gggccggggc gttgtcagtg gcggctggca gactgatcgg
     3421 gtgaccagga ccgagaccgc cggacagtcc ggcaacgatt ccaccagccc gttccggccc
     3481 gacgagagcg accgggacgc ggccttgcag ttcgtgctgc cgctcgtcgt tcggctggag
     3541 cgggccgatc ccccggcgcg tacggacgcg ctggagacgg cggcccgggc ggtgctggtg
     3601 ctgctgtcgg acgagcggtc ggccggggac ggcgagtggg cggcggcggt gcgggactgg
     3661 caggacgcca ggatccggaa ggtggtgcgg cgggcccgcg gcgcggagtg gcggcgggcc
     3721 gaggcgctgc ccgggatcac ggtcaccggg cgccgggccg aggtgcgggt ctatcccccg
     3781 gtgctgctgg acggctggcc caaggacctg gtgaagctcc aggtctccgg cacggagctg
     3841 gacgaccccg agccgcccgg gccgcccgcc gacggcacgc cggtgctgtg gctgaacccg
     3901 gcactggaga tgtcggcggg caaggagatg gcgcaggccg gccacggcgc ccagttggcg
     3961 tggtgggagc tttccgaggc ggagcgggcg gcctggcgcg cggccgggtt cccgctggcc
     4021 gtccgctccg cggatcccgc ccgctgggag gcgctgaccc gcagcgggct gcccgtggtc
     4081 cgcgatgccg ggttcacgga gatcgcgccg ggctcctgca ccgtcgtcgc ggaccacccg
     4141 gcgctgcgcg acgcgctgtc gcgggggcgg tgagttcggc gtgctgacct ccctgctcgg
     4201 cttcaccctg gtggccgcgg tgctgaccgt ttcgcccggc cccgacttcg cggtggtcct
     4261 gcggaccgcg ctcggctcgg ggcggcgcgc cgcgctgtgc agtgcgctgg gcatagccgc
     4321 cggatgcttc gtgtgggggc tggccggtgc ggtcgggctg accgcgatgc tcgccgcgtc
     4381 gacgacggcg tacgacgtcc tgcgggtggc cggcgcgctg tatctgatgt ggctcgggat
     4441 acaggcgctg cgcagcgccc ggcgcctacg gaccgacggt gctcaggagg ccggggcggc
     4501 gcccgccgcg gcccgggggc ggtcccgtga cggcgagggg ccgcggccgg cgaccccgct
     4561 gcgggcgttg cggacgggcc tgttgaccaa tgtcctcaac cccaaggtcg gggtggtcta
     4621 catgtcgctg ctgccgcagt tcatcccgca cggcgcgccg gtgctcgcga cgacgctgct
     4681 gctggtcgcc gtgcatgccg cgctgggcgt gctgtggctc ggcgggatcg ccgtcgcggt
     4741 gcaccgcgcg cgggccgtct tccagcggcc gcgcatacgg cggcggctgg atcaggccac
     4801 cggcggggtg ctgctggcgc tgggggccgc ggtggggttc gagaccgccc gctgaccggc
     4861 ggaagcggcc ggcggattcc catggggcgg cgggggcccc ggcgggaagc tcaaatgaag
     4921 ctctgaactc ggcaggtgca gggttcggcc cgtcatgacg tggccatgag taccccgacc
     4981 acggagcgag agggggaacc atggtgcgtc aaggaacgga catcgagcgc ctggggacgg
     5041 gcatcggctg gcggccggaa atcgccgcgg agatcgccgg gctacccggt gtggactggg
     5101 tcgaggtggt ggcggagaac atctgccccg gccatctccc ggtcgccctg cgggagttgc
     5161 gcgagcgcgg gacgaccgtc gtcccgcacg gtgtctcgct cggtctcggc ggcgccgacc
     5221 gtccggacgc cggccggctg gccgccctcg ccgagcgcgc cgaggtactg ggcgccccgc
     5281 tggtcaccga gcacatcgcg ttcgtccggg ccggcgggcc gctgaccgcc tcgccgctga
     5341 tggaggccgg gcatctgctg ccggtccccc gcacccgcga cgcgctggac gtgctctgcg
     5401 agaacgtccg catcgcccag gacgccctgc cggtgccgct ggccctcgag aacatcgcgg
     5461 ccctggtcaa ctggccggac gaggagctca ccgaggggca gttcctggcc gaactggtcg
     5521 agcggacggg ggtgcggctg ctcatcgacg tcgccaacct gcacaccaac cacgtcaacc
     5581 gcggtgagga cccggccgtg gcgctggacg agctgccggt cgaggcgatc gcgtacgtcc
     5641 atgtggcggg cggcgtggag cgcgacgggg tctggcacga cagccacgcc catccggtga
     5701 cccggccggt gctggacgtg ctcgccgagc tgtgtgcccg gatcaccccg cccggtgtgc
     5761 tgctggagcg cgatgacgac ttccccgagg ccggcgagct ggccggggaa ctcgacgcga
     5821 tccgcaccgt cctgaaggag gcccgccatg tccaggcggc ttgagcccgt acgggagccg
     5881 ggcaccggct cccgctccgc cgtggccccc accgcctcgg cccccgccat ccccgccacc
     5941 ggcaccgacg cggcgcggca gcggctggcg ctggcgcaga ccgcgctgct ctccgcgctg
     6001 gtcgccggca ccccggcgcc cgagggcttc gaccgcaccc ggctgggcgt gcagagccgg
     6061 gcgctgaccg ccaagcggtc cgcggtggtc gccaaggtcg cgcccgaact acccgagatc
     6121 ctcggcgacg actaccgccc ggcgttcgcc ggctatgccc gcaaccggcc gatgcgcggc
     6181 ggctaccgcc aggacgcact ggacttcgcg gcgcacctgc tggcccaggg ccgccccgcg
     6241 gaccccgccg cccggcagcg gctgacccgc tggtggcggg accgcgcggg ccccaccccg
     6301 ccgtccgccg ggcccgcggc ccggctcgcc cgcgccgtcc acgccgtccg ccaggcgctg
     6361 aagggaaggt ggcggtgatg tccttcacgc aagcggtgta cgtgggcctc ggtgcctcct
     6421 cgctggtact gctgctcggc acggtcctgg tgcggcgcgg caccccgtcc gtccgcaggg
     6481 gcgaggcgca ggcccgcaac gtgtgggaga tggcgttcct cgccggcggc cccgggcggg
     6541 tggtggacgc cgcgctcgcc gggatgtgcg aggacggccg gctggcggtg ggcggcccgg
     6601 gcgtggtgac cgtccgccag gccaccgcac agcacgcggt cgagtccgcc gtcctggacg
     6661 cggtcgcgcg gacgcccggc ggggcgctgg ccgcggtgcg cgccgaggcg atgcgcagcc
     6721 ccgcggtcca gggcgtcggc gaccggctgg cggcccgcgg gctgatgcgc catccgcggc
     6781 ggggccaggg ctggcgccag ttcgcgggcg cccagcgggc cgtgtgcgtg gcgctgttcg
     6841 tcctcggggt gctgctctcg atcgcggacg ccggccctga cgacggtgtc gacgaggggt
     6901 tcggcccacc ggtggtgatc acgatcatgc ccgcgctgat cggcggtctg atcgtggcct
     6961 cgctgtgccg gagggtcttc cggaaccgcg tcaccaaggc gggcgccctc gcggtgcgct
     7021 ccgcgcgggc cacccacgcc cagggcggca cgggcgacgc atggcaggcc gccgggctgg
     7081 tggtggcgct cggcggtacg gcgctgatag cggacgaact gctgcgccag cagttcgagg
     7141 aggcccagcg ggccaccgca ctggccaccg ccggcggctc gtcgtcgtcc ggcagcgcgt
     7201 cgtccggcta tgactccggg ggcggcgcgg gcggcgacag ctccttctgg tgcggctcgt
     7261 ccgacggcgg ctccggttcg tcctgcggct ccggttcgtc ctgcggcggc tcgtcctgcg
     7321 gctccggttc gtcgtgcggc ggcggttcga gttgcggggg cgggtcctcg tgcggcggcg
     7381 gctcgtcctg cggcgggggc tccggctgcg gaagcagctg agcgacggcg gcgcaggggg
     7441 cgcctccccg atcggaccgg ctccgggagc cgaccacggc cggcaggccc tacggccggc
     7501 aggctcctgc gcccgggaag gcccctacgc ccgaagctgt ccggtttgcg ggccgcaccg
     7561 gccctgatca gggacgggag cacgtgatgt ggtccacaca aggggaaaat taatggccat
     7621 tgtcatggac cttcccgtag aaaatggtcc atgtggctcc tcttcctcct cgtcgccgcg
     7681 gtcgcggcgg tgttctcctg tgcgcgggtg tgccgggccg cggtcgccgc ggcccggacc
     7741 ggacggccgg cccggccgcc ggcccccgct ccggccccgg acccggccgg tgacgacggg
     7801 cacgcgcccg gactgaccct ctacgagatg gcctacctgt ccggcgggcc gcagcggctc
     7861 gccgacgtgg tgctggtgct gatggcccag aagcggcggc tgctgctcgc ccacaccggc
     7921 tgggccaccg tcgtcgagcc ggtcgcgcag gacccggtcg agcgggcgac actcagcgcg
     7981 ataggcccgg acggacagcg cccgataccc gcggtgcggg aggccctggt ggccgacgag
     8041 gcggtgcggg cggtggccga caagctggtg gccgacgggc tggcgctgcc ggccgcggcc
     8101 cgccccggca tccgcagcgg gatccgccag gtgtgggccg ccgccctcct ggtcgtactg
     8161 accgccgccg cggcctactg gatggcgccg cccggcacgg acaccgcgct gctcctgggg
     8221 tggttctcgc tgccgctggt gctctgcctc gggaccctgg ccgtggcccg cttcgagctc
     8281 cacccgtacc gggactgggc caccgacgag ggcgaacagc gccttccggg gaaggccgat
     8341 ccgcccggct cacggctccc ctccgcccct ctggcacccc tcacctccct catctccctc
     8401 gccctgcacg gccccagcgc cctcaccgat cccgcccttc gcgccgcgct ccactcttcc
     8461 ggggcatagc gtccgacacc ggtgcccggt gctttacatc gccaatcacc gccccaacta
     8521 tcccttttgt atgcacacac gaagggacgg ccagtgagag cacttgcgtt gtacggcacc
     8581 atcgggtcgc tgatcgtgac cgccctcgcc actgccccgg cgggcggcac cacgcaggcg
     8641 gccggggcac cgcccgcgcc cgtcgcgcac cgcatcgact tcggccggtg cgcgccggtg
     8701 gaacacctgc cgtcgtccgt cgagtgcggc aagctcaccg tccccctgga ctacgcgcgc
     8761 cccgacggca agaagatcca gctcacggtc agccgggcgc gcgccaccgc gcaccgcgag
     8821 cggcagggct cgctggtgtt caacccgggc ggccccggcg cctccagcct ggagttcccg
     8881 ctctacggcg cactgcccaa atggagcagg ctggcccggg cctacgactt cgtcggctac
     8941 gcgccgcgcg gggtgggccg ttcggcgccg ctgtcgtgcc aggaccccaa ggaattcgcc
     9001 aaggcgccca ccgacagccg gatgtacccc gacgcggcgt tcaagcggaa gaagcgggcg
     9061 caggccatgg cgtacgcccg cggctgcgcc cggcgggccg gcgcggacct gccgttctac
     9121 aactcggtca acaacgcccg ggacctggac atgctgcggg ccgccctcgg cgagaagaag
     9181 ctgaccttca tcggcgcctc gtacgggacg tacttcggcg cggtctacgc gacgctctac
     9241 cccggccacg tgcggcggat gatcttcgac agcgtggtca acccggagcc gcggcagatc
     9301 tggtaccgca acaacctcga ccaggacatc gccttcgagc gccgctgggg cgactggctc
     9361 ggctgggtcg ccaagcacga cgaccactac cacctcggca cgaccaagaa ggccgtgcag
     9421 caggcgtacg acacggtcgc cgggaagctg cgccgccacc cggtcgacgg caagatcggc
     9481 gccggcgagc tgcagtccgc cttcctcaag accggctaca acgacggctt ctggccgctg
     9541 cgcgccgagg cgctcgcgag ctatctgcac ggtgacccca agccgctgat cgcccaggcc
     9601 ctgccccagc agaccggcgc caaggacgcg gagaacgcca acgcggtcta cacggccgtg
     9661 gagtgcaacg acgcgccttg gccgcggtcc tggcggacct gggaccggga caacaccgac
     9721 gtcgccgaga tcgcaccgtt cgagacctgg gacaacgcct ggatgaacct gccgtgcgcg
     9781 ttctggccgg aacggacgca gtccgcggcg gccggggtcg aggcggcggc ccgccgggtg
     9841 gggcagggct cgggccagga gctggcggac gccgccggcc acgccgccgc caccgagctg
     9901 gacggtgcgc tgcacggccg gcgcctcccg ctcgacgtcc acacggaacc cggtgcgctg
     9961 cccccggtgc tgctgctggc ggcggagcgg gacgcggcga cgccgtaccg gggcgcgctg
    10021 gagctgcagc gccggctgcc cggctcgtcg ctggtcaccg agaacggcgc gggcacgcac
    10081 ggcatcggct tcggcgagaa cgactgcgtg aaccgctacg ccgaggcgta tctgctgcac
    10141 gggaagatcc cggcgcacaa ggtgtactgc gcgccgcgcg cggagccggt gcccggcgag
    10201 cagacgaccc gggcccagca cgcgcgcccg gggaagtagc cgcgcacgac cgacgagcgg
    10261 gccgccccgg ggcaggggcg gcccgctcgt ccgtgccggg cggtcaggcc agcccggcga
    10321 ccagctcggc gaccgccttg cgacggccgg tgtagaacgg gacctcctcg cggacgtgca
    10381 tccgcgcctc ggagccgcgc agatgacgca tcaggtcgac gatgcggtag agctcgtcgg
    10441 cctcgaaggc gagcacccac tcgtagtcac cgagcgagaa cgacggcacg gtgttggcgc
    10501 ggacgtccgg gaagccgcgg gccatcttgc cgtggtcggc gagcatccgg cggcggtcct
    10561 cgtcggggag gaggtaccac tcgtaggagc gcacgaaggg gtagacgctc acataggcgc
    10621 gcggccgctc gtcggccagg aaggccggga tgtgcgactt gttgaactcg gcggggcggt
    10681 gcagcgccat gttggaccac accgggtcga gcgcgcggcc gagccgggtg cggcggaaga
    10741 ggttgtaggc gtcctggagg gcgtccgagc tctccgagtg ccaccagatc atgacgtcgg
    10801 cgtcggcgcg caggccggag acgtcgtagg tgccgcggac caccacgtcc ttggcggcga
    10861 gctgcgcgaa cagctcctcg acctcgtcgg cgtagccggt gcggtcctcg gggaggacgt
    10921 cgcgcagctt gaacaccgac cagagggtgt agcggatgac ctcgttcagg tccttggcct
    10981 tcttgccggc gttcggtgcc ttgtcgggct ccgcagggtt ctgcggtgcg gaagcggggg
    11041 tggaagcgtc tgtcatgtcg tccattctca ctcgccgggg cggccggccg gccgcagggt
    11101 gccgaggatg tcctcggcgg ccttgcgggc cgaggcgata caggccggga tgccgacgcc
    11161 gtcgtagagc gcaccgcaca cccgcaggcc cggcagccgc ccgacggcct cccggatgcg
    11221 ggccacccgc tccagatggc cgaccgggta ctggggcagg ccgccctccc aacgggtgat
    11281 ccgggcggcg accggcgcgg cggtcagccc gatcgcctcg cgcaggtcgg cgcgggccag
    11341 ggcggccagc tcggcgtcct cgcgcgccag gtcggcct
//
