LOCUS       NZ_AP026073            10984 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4333791
            Orig. end                         :: 4344775
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="15"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(423..436)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
     gene            508..1197
                     /locus_tag="HEK616_RS19320"
                     /old_locus_tag="HEK616_39410"
     CDS             508..1197
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092047.1"
                     /locus_tag="HEK616_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39410"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_261954194.1"
                     /transl_table=11
                     /translation="MPLEALRPSPLVEQAAQRLRGQITGGDWPVGTRLPGETTLAKTLG
                     VGRSTVREALRALAGAGLVQPRQGAGVFVIATEPTEDWPARLRRAAVGDVYEVRAMVEV
                     QAAQLAAVRRTDDDVAALRAALEGRRTAAGSGTAAFVDADIALHAAVVAAAHNPVLTDL
                     FTEFVPALRQGLIDLVELLDLRGRDPQPGDARHAALVDAVADGDGARASRILREEFDET
                     LGQLAGR"
     gene            complement(1239..3761)
                     /locus_tag="HEK616_RS19325"
                     /old_locus_tag="HEK616_39420"
     CDS             complement(1239..3761)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003992312.1"
                     /locus_tag="HEK616_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39420"
                     /product="MFS transporter"
                     /protein_id="WP_261954195.1"
                     /transl_table=11
                     /translation="MTQTTTDQLPGRGVGAGGAGAVGAAETPAVSGPAADGERRAGGAI
                     VPVLAFAGIVVAVMQTLLVPVIKDLPVLLDTAPSNATWVMTATLLAGAVATPIMGRLGD
                     LYGKRRMLLTSLAVMVVGSVVCGLTSDLLTMIVGRVLQGFAMGAIPLGIGIMRDALPRE
                     RLGSAMGLMSSSIGVGGGLALPAAALVAQHADWHSLFFGAAGLGVLSILLTLLLVPETP
                     VRAPGRFDVAGAVGLSAGLVALLLPITKGSDWGWSSPTTLGLFGIAVLVLVLWGVMELR
                     IADPLVDLRTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPESTGYGLGESMVVAG
                     LCVAPLGLTMMLVAPVYARIAARRGPKATLLLGMLVIAVGYGAGIGLMNAAWQTVIIAV
                     VVGAGIGLAYSSLPALIIGAVDASETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSQRM
                     GPATVPTMAGFRVSFLIATAAVVLGVALASFLPSQRNPPRPTLVAQSTDDDADHGAGQG
                     TGDNNNDDDNDDNDDDNDDNNNDRGIDQGADADPARGTGQGGDEGGSASDPRPGFRGRV
                     LGSHGGPVPGASVTLIDHQGRQAGVATAAADGRYALAAPAAGTYVLTAAAPGHAPYAAS
                     ATYRGAGATAETDLVLTAGGRLHGTLCGGPGGTPLAGGGIVVTDATGEVVARTASGAEG
                     RWEVTELPPGGYTLVLSAPGHQPQARAVEVSGGAGQERQDVRLRPAATVRGTVRGPGGR
                     PLADAAVTLLADGTVTGHTVTGPDGAFAFSGLCGSRYTLTAAGYPPHAAPISLTGGAEE
                     TLDLTLTQPSAADPQPSTADPRPSVTDG"
     misc_feature    3812..3828
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.12"
                     /tool="antismash"
     misc_feature    3864..3878
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     misc_feature    complement(3900..3916)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
     misc_feature    3925..3940
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    complement(3926..3939)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     misc_feature    complement(4029..4047)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.47"
                     /tool="antismash"
     gene            complement(4140..4826)
                     /locus_tag="HEK616_RS19330"
                     /old_locus_tag="HEK616_39430"
     CDS             complement(4140..4826)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608007.1"
                     /locus_tag="HEK616_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39430"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_261954196.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILESAAAVFDEQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAILQEQVVDIAVEPQPIKLQEFVDSGHVLAFRLRTDPIQRGAARLA
                     VEQGSNHLDRRQSMLAWSQFVEALFDEAKGRGEVLESVDVRDTAELFVGAFAGLQMMSQ
                     AFSNRADLSHRLTVFFQHTLPSIAVPAILAKLHLDPERGEKLNAELQQKAQSAAGTGTG
                     LDVVAS"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            5001..5984
                     /locus_tag="HEK616_RS19335"
                     /old_locus_tag="HEK616_39440"
     CDS             5001..5984
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="HEK616_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39440"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_261954197.1"
                     /sec_met_domain="AfsA (E-value: 4.6e-18, bitscore: 63.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMHVSGTAQPTTPQQLHHPRNPGDTLPPPSRTLVPREWVHRP
                     LLDDVLVTSWRRVDDAHFSLTAEWPHDHGYFTTPHGRHHPILTGETIRQAGLLICHTEL
                     GVPVGHHFILGDLVYTMDPEHLAVGDRPTSLTIDVTCSKLRMRAGTLVSGRFDMTIRKA
                     GRIVATGHSNVTVTSPGVYHRIRGDRLTARRGLGPLLPPVAPHLTGSATEHDVLLSPTD
                     RPDRWVLRVAPGHAAVVNPANDHIPGMVLLDAAQQAARARTAPGGFVPYAVGTEFRRYA
                     EHGVACEIEARRVPSALPGSTTVQVTGTQQDEPVFVTTLTALDARD"
     gene            6103..6867
                     /locus_tag="HEK616_RS19340"
                     /old_locus_tag="HEK616_39450"
     CDS             6103..6867
                     /NRPS_PKS="Domain: PKS_KR (7-172). E-value: 1.1e-07. Score:
                     23.9. Matches aSDomain:
                     nrpspksdomains_HEK616_RS19340_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="HEK616_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39450"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_261954198.1"
                     /transl_table=11
                     /translation="MGDLNGKTALVTGASRGIGRGIAQRLAQDGALVAVHYGSNDIAAK
                     ETVEHIREAGGQAFIIGAELGVPGDAEALFSAFDDGLAAHGARAGLDILVNNAAISLPG
                     HIDEVTPEEFDRILAVNTKAPFFVTQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVS
                     KGAVETLTLTLAKDLGERGITVNTIAPGFIETDMNAAMRATPEAHAALAAISVFNRLGR
                     PADVADVAAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        6124..6618
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS19340_PKS_KR.1"
                     /evalue="1.10E-07"
                     /label="HEK616_RS19340_PKS_KR.1"
                     /locus_tag="HEK616_RS19340"
                     /protein_end="172"
                     /protein_start="7"
                     /score="23.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGASRGIGRGIAQRLAQDGALVAVHYGSNDIAAKETVEHIR
                     EAGGQAFIIGAELGVPGDAEALFSAFDDGLAAHGARAGLDILVNNAAISLPGHIDEVTP
                     EEFDRILAVNTKAPFFVTQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVSKGAVETL
                     TL"
     CDS_motif       6130..6195
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HEK616_RS19340_0001"
                     /evalue="1.20E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="HEK616_RS19340"
                     /protein_end="31"
                     /protein_start="9"
                     /score="14.7"
                     /tool="antismash"
                     /translation="LVTGASRGIGRGIAQRLAQDGA"
     gene            complement(6888..7409)
                     /locus_tag="HEK616_RS19345"
                     /old_locus_tag="HEK616_39460"
     CDS             complement(6888..7409)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="HEK616_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39460"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_261957300.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGEESATAPGGTALVSLSLPGQKGGAVVQ
                     DGWVRDPAAFDGIARQLRAYFDGTLTRFDIEYADTAATRGTAFQRRVWQALEAVPYGTT
                     TTYGRVAEAIGAAPAAVRAVGTAIGRNPLLIVRPCHRVIAANGSLSGYAAGPERKRQLL
                     GLEGAAPRPL"
     gene            complement(7540..7767)
                     /locus_tag="HEK616_RS19350"
                     /old_locus_tag="HEK616_39470"
     CDS             complement(7540..7767)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="HEK616_RS19350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39470"
                     /product="hypothetical protein"
                     /protein_id="WP_030417114.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIRLDDPRLVEWRGGSLDDWPMPSA"
     gene            7890..9095
                     /locus_tag="HEK616_RS19355"
                     /old_locus_tag="HEK616_39480"
     CDS             7890..9095
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031840.1"
                     /locus_tag="HEK616_RS19355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39480"
                     /product="cytochrome P450 family protein"
                     /protein_id="WP_261954199.1"
                     /sec_met_domain="p450 (E-value: 5.1e-18, bitscore: 63.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRNASGADPDPLASPEYFRDPYPLLAAVRSRGPVQRIRTANGRT
                     TWLVTGWAEARAALADARLSKDTARYFAGRPSNRDLAPAVSRTLLATDPPDHGRLRKLA
                     LAAFTPAAVARLEPRIRVLAEELADALAERVGADLVEGFAVPLPIAVISELLGVPEPDR
                     AAVRRWSNELFAAAAPDTVDRASHALSGYMTRLIADRRGAPGDDVLSGLIAARDEGDRL
                     SEPELVSLAVLLVVAGHETTANLIGNGMVTLLRDDALRARLRADPGLLPAAVEEFLRHD
                     GPLTVATFRYATEPLVLGGATIAAGDVVQVAPGAANRDPARFDAPDSVRLGRAGGHLSF
                     GHGPHHCLGAALARVEARIAFEVLLTRWTDIRPAGAGPDAVEWRRTRLMRGPARLPVTF
                     VR"
     gene            complement(9189..10085)
                     /locus_tag="HEK616_RS19360"
                     /old_locus_tag="HEK616_39490"
     CDS             complement(9189..10085)
                     /EC_number="1.1.1.60"
                     /GO_function="GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092617.1"
                     /locus_tag="HEK616_RS19360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39490"
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="WP_261954200.1"
                     /transl_table=11
                     /translation="MSNLPKIAWIGLGIMGSPMAENLIKAGYAVTGYTLEQDKLERLAG
                     NGGTAATSIAEAVADADVVITMVPASPQVEAIAYGPDGILENARRGALLIDMSSITPQT
                     SIDLARAAGEKGIRVLDAPVSGGETGAIEAVLSIMVGGEQADFDAARPILEALGKTIVL
                     CGPHGSGQTVKAANQLIVAVNIQACAEAVVFLEKSGVDLTAALDVLNGGLAGSTVLTRK
                     KDNFKNRDFAPGFRIDLHHKDMGIVTDAARTVGAALPVGSVVAQLVAALRTQGDGGLDH
                     SALLRGVERLSGYPTEG"
     misc_feature    complement(10106..10115)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 17.75"
                     /tool="antismash"
     gene            complement(10140..10979)
                     /locus_tag="HEK616_RS19365"
                     /old_locus_tag="HEK616_39500"
     CDS             complement(10140..10979)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537603.1"
                     /locus_tag="HEK616_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_39500"
                     /product="TIM barrel protein"
                     /protein_id="WP_261954201.1"
                     /transl_table=11
                     /translation="MAFTDTRFNVNLSILFTELPLLERPAAARAAGFTAVELWWPWVDA
                     PVPEQAELDALRDALTDAGTRLVGLNFYAGQLPGPDRGALSIPGEESEKFRANVPVAIE
                     FAKSLGCTTFNALYGNRVDGVPEEEQDRLALENLAFAARAVAEVDGTLLIEALNAPESP
                     KCPIVSAPKAIETVDAVNAATGLDNARFLMDLYHLSMNGEDLDEVIDRYTAKTAHVQIA
                     DNPGRGAPGTGELDFDALLDRLKKAGYDGWIGLEYKPGDTPSASSFAWLPAPLRAAP"
ORIGIN
        1 atcggttcgg cgagcgcctg gttgccgtcg cgcaggtcga ccggcaccca cagcggggcc
       61 cgctcgatcc gggcggcggg ccaggcgcgg gtggtgaccg gtacctcgac gcgttggtgc
      121 gcggcccggt agcggtggga cggcatcgcg ctgtggcgct gcgggttcca cgccggcgcg
      181 tcggcgggca ccggaccggt gggcgtgcgg agggcgggag cgggggcgag tgcgagggag
      241 gtgccggtat cggtgcggcc ggtacgggag ttgctggtgt cgcggtcgaa gccggacatc
      301 tcgtggggga tctcgtggga catcgcgttc cttcaacagg agccggagaa agaccggcag
      361 caccgaaccc cgcggcgggg tgccggtcgc gtcaggcccc gccgcggcgg ccgagaagga
      421 gaccacgcag cgtcatgccg ggtacagtaa ccacacatca actcctcaga caacctgagt
      481 tgttcggaag gtgcgaggta ccacggcatg ccgctcgaag cgctccgccc cagccccctg
      541 gtcgaacagg ccgcccagcg gctgcggggc cagatcaccg ggggcgactg gccggtgggc
      601 acccggcttc ccggagagac caccctggcc aagaccctcg gcgtcggccg ctcgacggtg
      661 cgcgaggccc tacgggcgct ggccggtgcg ggactggtcc agccgcgcca gggcgccggg
      721 gtgttcgtga tcgcgaccga gcccaccgag gactggcccg cccggctgcg ccgcgcggcc
      781 gtcggtgacg tctacgaggt gcgcgccatg gtggaggtcc aggccgccca actggccgcc
      841 gtccggcgca ccgacgacga tgtcgccgcc ctgcgcgccg ccctggaggg ccgccgtacc
      901 gccgccgggt ccggcaccgc ggcgttcgtg gacgcggaca tcgccctgca cgcggccgtc
      961 gtcgcggccg cgcacaaccc cgtactgacc gatctcttca cggagttcgt gcccgccctg
     1021 cgccaggggt tgatcgacct cgtcgagctg ctggacctgc gcggccggga cccgcagccc
     1081 ggcgatgccc ggcacgcggc gctggtcgac gcggtcgcgg acggcgacgg cgcgagggcg
     1141 agccggatcc tgcgggagga attcgacgag acgctggggc agttggcggg gcggtgaagg
     1201 gaggggcgcg tacgggccgg acgtacgcgc ccctcggctc agccgtctgt gacggagggc
     1261 cgggggtcgg cggtggacgg ctggggatcg gccgctgacg gctgggtgag ggtcaggtcg
     1321 agggtctcct cggcgccgcc ggtgagggat atcggggcgg cgtgcggcgg gtagccggcc
     1381 gcggtgaggg tgtaccggct cccgcacaga ccggagaagg cgaacgcgcc gtccggtccc
     1441 gtgacggtgt gcccggtgac cgtgccgtcc gcgaggaggg tcaccgcggc gtcggccagc
     1501 gggcggccgc ccggcccgcg caccgtgccg cggacggtgg cggccggccg cagccggacg
     1561 tcctggcgct cctggccggc gccaccggac acctccacgg cacgggcctg cggctggtgg
     1621 ccgggggcgc tgaggaccag ggtgtagccg ccgggcggga gttcggtcac ctcccaccgc
     1681 ccctcggccc cggaggccgt acgggccacc acctcacccg tcgcgtcggt gacgacgatg
     1741 ccgccgccgg cgagcggggt gcccccgggg ccgccgcaca gcgtgccgtg gagccggcca
     1801 ccggcggtga ggaccagatc cgtctcggcg gtcgcgcccg caccgcggta ggtcgcggag
     1861 gcggcgtacg gggcgtgtcc gggcgcggcg gcggtcagga cgtacgtacc ggccgccggg
     1921 gcggcgagcg cataccgccc gtcggccgcg gcggtggcga caccggcctg ccgcccctgg
     1981 tggtcgatca gcgtgacgct cgccccgggg accggcccgc cgtgggaccc gaggacacgg
     2041 ccccggaatc cgggccgcgg gtcgctcgcg gacccaccct cgtcgccgcc ctggccggtt
     2101 ccgcgggcgg ggtcggcgtc ggcgccctgg tcgatcccgc ggtcgttgtt gttgtcgtcg
     2161 ttgtcgtcgt cgttgtcgtc gttgtcgtcg tcgttgttgt tgtcgcccgt gccctgaccg
     2221 gccccgtggt cggcgtcgtc gtcggtgctc tgcgcgacca gggtcggccg gggcggattg
     2281 cgctgcgacg gcaggaagga ggccagcgcc acgccgagga cgaccgcggc cgtggcgatc
     2341 aggaaggaga cccggaagcc ggccatcgtg gggaccgtgg ccgggcccat ccgctgggac
     2401 atgtgagcca gcaccatgcc gatcaccgcg ctggagaccg aggtgccgat cgaacgcatc
     2461 agggtgttca ggccgttggc cgcgccggtc tcggaggcgt cgacggcgcc gatgatcagc
     2521 gcgggcagcg aggagtacgc cagcccgatg ccggcgccga cgaccacggc gatgatgacg
     2581 gtctgccagg cggcgttcat caggccgatg cccgcgccgt atccgacggc gatgaccagc
     2641 atgcccagca gcagcgtcgc cttcgggccg cgccgggccg cgatccgcgc gtacaccggc
     2701 gcgaccagca tcatggtcag ccccaacggc gccacgcaca ggccggcgac caccatggac
     2761 tcgcccaggc cgtagccggt cgactcgggc agctgcagca gctgcggcag caccagcgag
     2821 atcgcgtaga aggccacccc gaccgtgatc gaggcgaggt tggtcagcag cacctcgcgg
     2881 cgggcggtgg tgcgcaggtc caccagcggg tcggcgatgc gcagttccat cacgccccac
     2941 agcaccagga ccagcaccgc gatcccgaac agcccgaggg tggtgggtga actccagccc
     3001 cagtcactgc ccttggtgat gggcagcagc agcgccacca gccccgcgga cagcccgacg
     3061 gcgcccgcca cgtcgaagcg gcccggggcg cggaccggcg tctcgggcac caggagcagg
     3121 gtgagcagga tggagagcac gccgagtccg gcggcgccga agaacaggct gtgccagtcg
     3181 gcgtgttggg cgacgagggc ggccgcgggc agcgcgagtc cgccgcccac gccgatcgag
     3241 gagctcatca gccccatggc cgagccgagc cgttcgcgcg gcagcgcgtc gcgcatgatg
     3301 ccgatgccga ggggtatcgc gcccatcgcg aagccctgca gcacccggcc gacgatcatc
     3361 gtgagcagat cgctggtgag cccgcagacc accgagccga cgaccatgac cgcgaggctg
     3421 gtcagcagca tccgccgctt gccgtagagg tcaccgagcc gccccatgat gggggtcgcg
     3481 acggcaccgg cgaggagagt ggccgtcatg acccaggtgg cgttgctcgg cgcggtgtcg
     3541 aggagcaccg gcaggtcctt gatgaccggt acgagcagcg tctgcatcac cgcgacgacg
     3601 atgccggcga aggcgaggac cgggacgatg gcaccgccgg ctctgcgctc tccgtccgcg
     3661 gccggtccgg acaccgcggg ggtctctgcg gcgcccacgg cacccgcgcc cccggcaccc
     3721 acgccccttc cggggagctg atccgtcgtc gtctgcgtca tgcgtgcggc ctccaggcgg
     3781 cggaaggact gtcgtcagaa gtcatgagga gtcatcaaga acggggcagg gcgccgaccc
     3841 ggcgggcgcc cttgatacgt gcatgacgaa cgtttccact cggcggacta ttccgcacgt
     3901 cgcgaggcat ccgcagcacc acacggtgat ctgcctcacg ccgcccaccg gaccgacgag
     3961 aaccagtgaa gcgcgcacca ctgacaatcc aacgcacgaa gaaggcgccg cggcctttcc
     4021 gcgacacctt cccgaggtct tcgcaatgcc tccggacctc gatcgatcaa caccgcgtgc
     4081 gcggtatgtt tccccgcccg aattccgcgt tttacgtgaa tcgtccggcg gcgcgatcct
     4141 cagctcgcga cgacatccag tccggtgccg gtccccgccg cggactgcgc cttctgctgc
     4201 agttcggcat tcagtttctc cccgcgctct gggtcgagat ggagcttggc gaggatggcg
     4261 ggcacggcga tgctgggcag cgtgtgctgg aagaaaacgg tcagccggtg ggagaggtcg
     4321 gcccggttgg aaaacgcctg cgacatcatc tgcaaaccgg cgaaggcacc gacgaagagc
     4381 tcggcggtgt cccgcacgtc aacgctctcc agcacctcgc cgcgcccctt cgcctcgtcg
     4441 aacaacgcct cgacgaactg gctccaggcg agcatcgact gcctgcggtc caggtgattg
     4501 gagccctgct cgacggccag ccgcgcggcc ccgcgctgaa tcgggtcggt acgcagtcgg
     4561 aaggccagca cgtgaccgga gtcgacgaat tcctgcagtt tgatcggctg cggctcgacg
     4621 gcgatgtcga ccacctgctc ctggaggatg gcgagcgcga gctgctcctt ggaagcgaaa
     4681 tggaaataca gggcgccctt cgtcacgccg gcgcgttcga ggacttcggc gatcgtcgcg
     4741 cggtcatagc cctgctcgtc gaacaccgcg gcggccgact ccaagatcac tcgccgggtc
     4801 ctgatcgcac ggtcctgttg tgccacagga cgcctcctgt ctctcgaaat ctgccatttc
     4861 cgccggtgtg gccaccacgt caagtcgccc cgccaccagc gtatttcccc cttccctcag
     4921 cccggcattg aaaataaaac cggatggtac gtatcttaac agcgatggac ctcatcccgt
     4981 agagacgcct gggggcagtc atgagcgaca cgatgcacgt cagcggcacc gctcagccca
     5041 cgaccccgca gcaactgcac cacccccgca accccggcga cacccttccg cccccctccc
     5101 gcaccctcgt cccccgggaa tgggtccatc gccccctcct cgacgacgtg ctggtcacct
     5161 cctggcggcg cgtggacgac gcgcacttct cgctgaccgc cgagtggccc cacgaccacg
     5221 ggtacttcac cactccccac ggccgtcatc atcccattct caccggcgag accatccgcc
     5281 aggcggggct gttgatctgc cacaccgaac tcggcgttcc ggtcggacac cacttcatcc
     5341 tcggcgacct cgtctacacc atggaccccg aacacctcgc cgtgggcgac cgacccacgt
     5401 ccctcacgat cgacgtcacc tgcagcaagc tgcggatgcg ggccggaacc ctggtgagcg
     5461 gccggttcga catgaccatc cgtaaggcgg gccggatcgt cgcgacgggc cactccaatg
     5521 tgaccgtcac ctcgcccggt gtctaccacc ggatccgcgg cgaccgcctc accgcccggc
     5581 gcggcctcgg ccccctgctg ccccccgtcg caccgcacct caccggcagc gccaccgagc
     5641 acgacgtcct gctctccccc accgaccggc ccgaccgctg ggtgctgcgc gtcgcccccg
     5701 gtcacgccgc cgtcgtcaac ccggcgaacg accacatccc cggaatggtg ctgctcgacg
     5761 ccgcccaaca ggccgcccgc gcccgcaccg cgcccggcgg cttcgtcccg tacgccgtcg
     5821 gcaccgaatt ccgccgctac gccgaacacg gcgtcgcctg tgagatcgag gcccggcgcg
     5881 tcccctcggc actgcccggc agcaccaccg tccaggtcac cggaacgcag caggacgagc
     5941 cggtcttcgt gaccaccctg acggcgctgg acgcccgcga ctgagcgaca cgctcacccg
     6001 ccacgtgctt cgcgcggcga agccggcacc gctgcaatcc cccgcatgcc acagtcatag
     6061 gcggccgcat gcccgcctca cgtacacgga aggccaacac tcatgggtga cctcaacggg
     6121 aagaccgcgc tggtgaccgg cgccagccgg ggcatcggcc ggggcatcgc ccagcgcctg
     6181 gcccaggacg gcgcgctggt cgccgtccac tacggcagca acgacatcgc cgccaaggag
     6241 accgtcgaac acatcagaga ggccggcggc caggccttca taatcggcgc ggaactgggg
     6301 gtcccgggcg acgccgaggc tctcttctcc gccttcgacg acgggctggc ggcgcacggc
     6361 gcacgggcgg gcctcgacat cctcgtcaac aacgccgcga tcagcctgcc gggacacatc
     6421 gacgaggtga cccccgagga gttcgaccgc atcctcgcgg tcaacaccaa ggcgccgttc
     6481 ttcgtcaccc agcacgggct gcgccggatg cgcgacggcg gacgcatcat caacatctcg
     6541 tcggcggtga ccactacggc cttcccgtcg accatcgcct acggcgtctc caagggcgcg
     6601 gtggagaccc tcaccctcac cctcgccaag gacctcggcg agcgcgggat cacggtgaac
     6661 accatcgccc ccggcttcat cgagacggac atgaacgccg cgatgcgggc gacgcccgag
     6721 gcgcatgccg cgctggcggc gatctcggtg ttcaaccggc tcggccgccc cgcggacgtc
     6781 gccgacgtcg ccgcgttcct cgcctccgac gactcccgct ggatcaccgg ccagcgcatc
     6841 gacgtcaccg gcgggtccat gctctgagcg cgcggcccgc gcccgggtca gagcgggcgc
     6901 ggcgcggcgc cctccaggcc gagcagctgc cgcttgcgtt ccggcccggc cgcgtagccc
     6961 gagagcgagc cgttcgcggc gatcacgcgg tggcagggcc gcacgatcag cagcgggttg
     7021 cggccgatcg cggtccccac cgcgcggacc gccgccggcg ccgccccgat cgcctcggcg
     7081 acccgcccgt aagtggtcgt ggtgccgtac ggaacggcct ccagggcctg ccagacacgc
     7141 cgctggaagg cggtgccacg ggtggccgcg gtgtcggcgt actcgatgtc gaagcgggtc
     7201 agcgtgccgt cgaagtaggc ccgcagctgc cgcgcgatcc cgtcgaacgc ggcggggtcg
     7261 cggacccagc cgtcctgtac gaccgcaccg cccttctgcc cgggcaggga cagcgagacg
     7321 agcgcggtac cgcccggtgc ggtggccgac tcctccccga ccagcaggag ttcgcccagc
     7381 gggctgtcga gggtggcgta gagcgtcatg agcgtcacgg aatccactgt gccgcaggtc
     7441 ggagcggtcc accggcggaa atcggacacc gagctgcgga cgtcgtgctg cgcggaccgc
     7501 gccgccgtgc cgggcggccg ggccgcggtg gccggtggct caggcggacg gcatcggcca
     7561 gtcgtccagc gagccgcccc gccattcgac cagccgcggg tcgtcgagcc ggatgtcctc
     7621 ggccgggccg gcgaccccgg cccggcggag gaattcggcc acgtcgccgc ggccgtgggc
     7681 caggccgacg atctgcccac gcacggtcac ccgtcgtcca ccggacggtg tcggcgggtg
     7741 cacgatgact ggcgggtgct cggtcatgcc tccagcgtgc gacgggcggc gcactcccgc
     7801 acgtcgaccg cctccagggg cggcaatcgt tgcccacgca aggactcccc gcaaagaatc
     7861 aaggactccc tacaaggaac ggtgttgccg tgcagagaaa cgcctcggga gcggatccgg
     7921 atcccctggc ctcgccggag tacttccggg acccgtaccc gctgctcgcc gccgtgcggt
     7981 cacgcggtcc cgtgcagcgg atcaggaccg cgaacggccg gacgacatgg ctggtgacgg
     8041 ggtgggcgga ggcgcgggcg gcgctggccg acgcccggct gtcgaaggac accgcccggt
     8101 acttcgccgg ccggccgagc aaccgcgacc tggcccccgc ggtcagccgc accctgctcg
     8161 cgaccgaccc gccggaccac ggccggctgc ggaaactggc cctggcggcc ttcaccccgg
     8221 cggccgtcgc acgcctggaa ccgcgcatcc gggtgctggc cgaggaactg gccgacgcgc
     8281 tggcggagcg tgtcggggcg gatctggtcg agggcttcgc ggtgccgctg ccgatcgccg
     8341 tgatcagcga gctgctgggg gtccccgagc cggaccgcgc ggcggtgcgg cgctggtcca
     8401 acgaactgtt cgccgcggcc gccccggaca ccgtggaccg tgcgtcacat gccctgagcg
     8461 gctatatgac ccggctgatc gcggaccggc gcggggcccc cggggacgat gtgctgagcg
     8521 gcctgatcgc cgcccgggac gagggcgatc ggctctccga acccgaactg gtctcgctgg
     8581 ccgtgctgtt ggtggtcgcc gggcacgaga cgacggccaa cctcatcggc aacggcatgg
     8641 tgaccctgct ccgcgacgac gccctgcgcg cccggctccg ggccgacccc gggttgctgc
     8701 ccgccgcggt cgaggaattc ctgcgccatg acggcccgct gaccgtcgcg accttccgct
     8761 acgccaccga accgctggtc ctcggcggcg ccacgatcgc cgcgggcgat gtcgtccagg
     8821 tcgcgcccgg cgccgccaac cgcgatccgg cacggttcga cgcaccggac tccgtacgac
     8881 tcggccgcgc gggcggacac ctctccttcg ggcacggccc gcaccactgc ctcggcgcgg
     8941 cactggcccg cgtcgaggcc cggatcgcgt tcgaggtgct gctgacgcgc tggaccgaca
     9001 tccgtcccgc gggcgcgggc ccggacgcgg tggaatggcg gcggacccgc ctgatgcgcg
     9061 gaccggcgcg cctgccggtg acgttcgtcc gctgagcccg gaagcacgag aaccggcgcc
     9121 gcgaccgcag tgcggcggtc gcggcgccgg ccgttcggga ccgtcgcggg acgtgcccgc
     9181 ggaccggctc agccttcggt ggggtatccg gagaggcgtt cgacgccgcg gaggagggcg
     9241 gagtggtcca ggccgccgtc gccctgggtg cgcagggcgg cgacgagttg ggcgacgacg
     9301 gagccgacgg gcagggcggc gccgacggtg cgggcggcgt cggtgacgat gcccatgtcc
     9361 ttgtggtgca ggtcgatgcg gaagccgggt gcgaagtcgc ggtttttgaa gttgtccttc
     9421 ttgcgtgtca ggacggtgga gccggccagg ccgccgttga ggacgtccag ggcggcggtc
     9481 aggtcgacgc cggacttctc caggaagacc acggcctcgg cgcaggcctg gatgttgacg
     9541 gcgacgatga gctggttggc ggccttgacg gtctgcccgg agccgtgcgg tccgcacagc
     9601 acgatggtct tgccgagggc ttcgaggatc gggcgggcgg cgtcgaagtc ggcctgctca
     9661 ccgccgacca tgatcgacag gacggcctcg atggcgccgg tctcgccgcc ggagacgggg
     9721 gcgtccagga cccggatgcc cttctcgccg gccgcgcggg ccaggtcgat ggaggtctgc
     9781 ggggtgatcg aggacatgtc gatcagcagc gcaccgcgcc gggcgttctc caggatgccg
     9841 tcggggccgt aggcgatggc ctcgacctgc ggggaggccg ggaccatggt gatcacgaca
     9901 tctgcgtcgg cgacggcctc ggcgatcgag gtggctgcgg tgccgccgtt gccggccagg
     9961 cgctcgagct tgtcctgctc gagggtgtag ccggtgaccg cgtaaccggc cttgatcagg
    10021 ttctcggcca tgggcgagcc catgatgccc aggccgatcc acgcgatctt gggcagattg
    10081 ctcatcgaaa taacctcttt cacactcaaa aatcagggga cagcgagcgg cggaccccgt
    10141 tatggggcgg cgcgcagcgg ggccggcagc caggcgaacg acgacgcact cggggtgtcg
    10201 ccgggcttgt actccaggcc gatccagccg tcgtagccgg ccttcttcag gcggtcgagc
    10261 agggcgtcga agtcgagttc gccggtgccg ggggcgccgc gcccggggtt gtcggcgatc
    10321 tgcacatgcg cggtcttggc ggtgtagcgg tcgatgacct cgtccaggtc ctcgccgttc
    10381 atcgacaggt ggtacaggtc catcaggaag cgggcgttgt ccaggccggt cgccgcgttc
    10441 accgcgtcca ccgtctcgat cgccttcggc gccgagacga tcgggcactt cggagactcg
    10501 ggcgcgttga gcgcctcgat gagcagcgtg ccgtcgacct cggccaccgc acgggcggcg
    10561 aaggcgaggt tctccagcgc gagccggtcc tgctcctcct ccgggacccc gtcgacgcgg
    10621 ttgccgtaca gggcgttgaa ggtcgtgcag cccagcgact tggcgaactc gatcgcgacg
    10681 ggcacgttcg cccggaactt ctccgactcc tcgcccggaa tggacagggc gccgcggtcc
    10741 gggccgggca gctggccggc gtagaagttc aggcccacca ggcgggtgcc ggcgtcggtc
    10801 agggcgtcgc gcagcgcgtc cagctccgcc tgctcgggga ccggggcgtc cacccacggc
    10861 caccacagct ccaccgccgt gaagcccgcc gcccgcgcgg ccgccggccg ctccagcaac
    10921 ggaagctcgg tgaacaggat cgacagattg acgttgaagc gcgtgtccgt gaaagccatg
    10981 aggc
//
