LOCUS       NZ_AP026073            10213 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2839352
            Orig. end                         :: 2849565
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10213
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10213
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10213
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5213](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5213
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            775..1683
                     /locus_tag="HEK616_RS12370"
                     /old_locus_tag="HEK616_25130"
     CDS             775..1683
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="HEK616_RS12370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25130"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_261952964.1"
                     /transl_table=11
                     /translation="MIEARRLHILRAVADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERSARGVRLTPAGDILLGHANAVLAQLERAESELAAYGSGEAGTVTVASFATGIG
                     LVVAPALTALAGCAPGIRVRVQDAEGDASLRMVLDRQVDVAVAVEYRGAPRADDPRLTR
                     VPLYAEPFDAVLPPGHRLAEADRVAVADLAGDPWIGPYPGNPCHDVVVLACEYAGFEPR
                     LEHSSDDFRAVVALASAAAGVALVPRSALRDMDLTGVVVRPLDDVAPTRRVFAAVRRGA
                     EDHPLFRPVLTALREAAAAQG"
     misc_feature    794..810
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            1784..3181
                     /locus_tag="HEK616_RS12375"
                     /old_locus_tag="HEK616_25140"
     CDS             1784..3181
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /GO_process="GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091737.1"
                     /locus_tag="HEK616_RS12375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25140"
                     /product="chloride channel protein"
                     /protein_id="WP_261952965.1"
                     /transl_table=11
                     /translation="MSVGPTGQSGPTGPGGPTGPDGTPPAAAPPDPYAPVRTRDYPKLL
                     LTAAVIGFPVCAIAFGFLTVVHALEPLLYDDLPRALGFAGTPLWWPLPVLALGGLLTGL
                     AIRHLPGNGGHEPADGLALGGPPGLSALPGVALASLASLAFGAVLGPEAPLIALGGGLA
                     AGAARLTGWGKDARAVAMVGSAGSFAAISALLGSPLLGAFLLMEVSGLAGPMLGVVLVP
                     GLLASGIGALVFTGLGDWTGLGTYSLALHDVPRAAGPTVPEFGWALVIGVAAACAGTGV
                     RRLARWLKARVIRHRVPATVLMGLVVGALAVGFSAATGKPAQELLFSGQSALGTLFHDS
                     AGYSMAALLLLLLGKGLAYCVSLSAFRGGPIFPALFLGAAGGVVLSHLPGLSLTPAFAM
                     GVGALAVAMLRLPMTSVLMATLLMGRSGLTVMPLVIVAVVVGYVVTLRLTPAPAAPGRT
                     APAPSAA"
     misc_feature    1811..1825
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            complement(3194..4222)
                     /locus_tag="HEK616_RS12380"
                     /old_locus_tag="HEK616_25150"
     CDS             complement(3194..4222)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606563.1"
                     /locus_tag="HEK616_RS12380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25150"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_261952966.1"
                     /transl_table=11
                     /translation="MEPVDALERIAFLLERQGAATYRVQAFRGAAAALGALSDDALRER
                     ARQGTLVRLKGLGPKTTRVVEEALAGVRPTYLARLEDEAGAPLVEGEQGQRLRRALRGD
                     CHLHSDWSDGGSPVDVMARAARDLGHEWCVLTDHSPRLTVARGLSAERLREQLELVAEV
                     NERLAPFRLLTGIECDILDDGSLDQEPELLERLDVVVASVHSKLRMDRAPMTRRMVAAV
                     RNPLVDVLGHCTGRLVTGRGRPESEFDADEVFAACAEHRTAVEINCRPERLDPPRRLLR
                     RALAAGTLFSIDTDAHAPGQLDWQIYGCARAEECGVPAERVINTWTERQLATWTATGRT
                     PR"
     misc_feature    complement(4512..4529)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.44"
                     /tool="antismash"
     gene            5001..5213
                     /locus_tag="HEK616_RS12385"
                     /old_locus_tag="HEK616_25160"
     CDS             5001..5213
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849411.1"
                     /locus_tag="HEK616_RS12385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25160"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_261952967.1"
                     /sec_met_domain="strepbact (E-value: 1.9e-23, bitscore:
                     81.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREIQTQEIADSELDNVAGGLVGNLAGTATSTLDGATGLNVGGTL
                     GTVTGTVEGLTGVNTGQVTGLVAGL"
     misc_feature    5382..5396
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.04"
                     /tool="antismash"
     gene            5414..6220
                     /locus_tag="HEK616_RS12390"
                     /old_locus_tag="HEK616_25170"
     CDS             5414..6220
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="HEK616_RS12390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25170"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_261952968.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEEIDLPVRFARPQGLLVLIVTVLCLLAAAVW
                     AVTGTVSSTLTAPGILTHGQGSYVLQSPVAGQVTAVLAQEGQRVSAGAPVLKVRTPRGT
                     AVVRTIAAGRLTTMVATIGSVVTTGADVASVERVAHTGDPLTATLYVPAENGASVPVGA
                     SVDLTVQSVPNQQYGVLRGRVKAVGRTAQTRQRIGAYLGSSQLGEQFSKAGPPVAVLVQ
                     LDRAPRNRSGYAWSTSDGPPYTVDSMTLASGAVHLAAQHPIDWLLP"
     misc_feature    complement(5437..5449)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            6217..8499
                     /locus_tag="HEK616_RS12395"
                     /old_locus_tag="HEK616_25180"
     CDS             6217..8499
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606566.1"
                     /locus_tag="HEK616_RS12395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_25180"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_261952969.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.1e-38, bitscore:
                     129.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTASQDSAPPQDRLPPAGRGRHRPEAASGARRRRATPPPAPKKVK
                     AVRTPTVLQMEAVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAARSY
                     GLQAKGMQMETAALAEVQAPAILFWEFNHYVVYDGMGRRMGRRGVHINDPDKGRRFVPT
                     EDFDTSFTGVALVFEPSESFRKGGRKPGVLGAVPARMRGTTGTLLAALLASLLLVAVGA
                     AVPALSRTYIDMFLIGKQTSLLGPLFASMAAMVALTAVLTGLQQANLLRGRIISSTLTS
                     ARFLRHLLRLPVTFFSQRSPADLVQRLQSNDAVAETLARDLAAAGVDGIVVVLYAVLLW
                     TYDPQLTVIGVGIALLNVVAMRIVIRLRATHTAKLRADTARLTNTAYTGLQLIETMKAT
                     GGENGYFRRWAGQHAATLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHI
                     SIGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADSLYTRPGSDGD
                     TRRLKGHVTLENITFGYSPLDKPLLTGFSLAVGPGRQVALVGGSGSGKSTVSRLISGLY
                     SPWEGTIRIDGQRLEDLSRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDEAVITA
                     LKDAALYDDVIARRPDGIHSRVDQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSAL
                     DAQTEQIIMDNLRRRGCACVVIAHRLSTVRDSDEIVVLDHGVVVERGRHEDLVAAGGAY
                     AELVKEH"
     misc_feature    9416..9430
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
ORIGIN
        1 ccaccgaggg cggcgcgggc cgcaagctga tcgtcaccga cggcgtcttc tccatggacg
       61 gctacgtggc gccgctgcgc gagatctgcg acctggccga cgcctacgac gcgatggtga
      121 tggtcgacga ctcgcacgcg gtcggcttcg tcggcgccgg cggccgcggc acccccgagc
      181 tccacggggt catggaccgc gtcgacatca tcaccggcac cctcggcaag gcgctcggcg
      241 gcgcctccgg cggctacgtc gcggcccgcg ccgagatcgt cgccctgctg cgccagcgct
      301 cgcggccgta cctcttctcc aactcgctcg ccccggtgat cgccgcggcc tcgctcaagg
      361 tcctcgacct gctggagtcc gccggtgacc tgcgcgagcg gctgcacgcc aacaccgcgc
      421 tgttccgctc ccggatgacc gaggagggct tcgacgtcct ccccggcgac cacgccatcg
      481 cccccgtcat gatcggcgac gcgtcccggg cggcccggct ggcggagctg ctgctggagc
      541 gcggggtgta cgtgatcggc ttctcgtacc cggtcgtgcc gcagggccag gcccgcatcc
      601 gcgtccagct gtccgccgcg cactccaccg aggacgtgca ccgtgcggtg gacgcattcg
      661 tagcggcgcg agccgcgatg aatgcctagg cgcgagccgc gatgaacgcc gaggcggggg
      721 ccgccacggg ggagtgaccc gcccggtccg tccgcgtcct gggacactgg ggacatgatc
      781 gaagcacgcc ggctgcacat cctccgtgcg gtggccgacc accgcacggt caccgccgcg
      841 gccgccgcgc tctacctcac gccctcggcg gtgtcccagc aactggccgc cctggaacag
      901 gagaccggcc accgcctggt ggagcgcagc gcccgcggtg tccggctgac gcccgcgggc
      961 gacatcctgc tcggccacgc caacgccgtc ctcgcccagc tggaacgcgc cgagtccgag
     1021 ctcgccgcgt acggctcggg cgaggccggc acggtcaccg tcgcgtcctt cgccaccggc
     1081 atcggcctgg tcgtcgcgcc cgcgctgacc gcgctggccg gctgcgcgcc cggcatacgg
     1141 gtccgcgtcc aggacgccga gggcgacgcc agcctgcgga tggtcctcga ccggcaggtc
     1201 gacgtcgcgg tggccgtcga ataccggggc gcgccccgcg cggacgatcc ccggctgacc
     1261 cgggtcccgc tctacgccga acccttcgac gcggtcctcc cgcccggcca ccgcctcgcc
     1321 gaggccgacc gggtcgccgt cgccgacctc gccggcgacc cctggatcgg cccctacccc
     1381 ggcaacccct gccacgacgt ggtggtcctg gcctgcgagt acgccggctt cgagccccgg
     1441 ctggaacact cctccgacga cttccgggcg gtggtcgcgc tcgcctcggc cgcggccggg
     1501 gtggcgctgg tgccccgctc ggcgctgcgc gacatggacc tgaccggagt cgtcgtccgc
     1561 cccctcgacg acgtcgcgcc cacccgccgg gtcttcgccg cggtacgccg cggggccgag
     1621 gaccacccgc tgttccgccc ggtcctgacc gcgctccggg aggcggcggc cgcgcagggc
     1681 tgaccggcgt tcgggtgctt cgggctgata cggacggcgt ccttccgggc cgcccgcccc
     1741 gcccgtggca ggctcgtccc ggttgcggcg aggaggcgag gcgatgtcgg tcggccccac
     1801 cggccagagc ggtcccaccg gccctggcgg ccccaccggc ccggacggca caccgcccgc
     1861 cgccgcgccc cccgacccct acgcgccggt gcgcacccgc gactacccga agctgctgct
     1921 caccgcggcg gtgatcgggt tcccggtctg tgcgatcgcg ttcggcttcc tcaccgtcgt
     1981 gcacgccctg gaaccgctgc tctacgacga cctgccgcgg gcgctgggct tcgccggcac
     2041 gccgctgtgg tggccgctgc cggtgctcgc cctcggcggg ctgctgaccg ggctcgccat
     2101 ccgccacctg cccgggaacg gcggccacga accggccgac gggctggcgc tcggcggccc
     2161 gcccgggctg tccgcactgc ccggcgtcgc gctcgcgtcc ctggcctcgc tcgccttcgg
     2221 cgcggtgctc ggtccggagg cgccgctgat cgccctgggc ggcgggctgg ccgcgggcgc
     2281 ggcccggctg accgggtggg gcaaggacgc ccgggcggtc gccatggtgg gatcggccgg
     2341 cagcttcgcc gcgatcagcg cactgctcgg gtccccgctg ctgggcgcgt tcctgctgat
     2401 ggaggtctcc ggcctggccg gcccgatgct cggagtggtg ctggtgccgg gcctgctcgc
     2461 gtcgggcatc ggggcgctgg tcttcaccgg gctgggcgac tggaccgggc tcggcacgta
     2521 ctccctcgcg ctgcacgacg tcccccgggc cgccgggccc accgtcccgg agttcggctg
     2581 ggccctggtc atcggggtgg ccgccgcgtg cgccggcacc ggggtccggc ggctggcgcg
     2641 gtggctcaag gcgcgggtga tccgccaccg ggtgccggcc acggtgctga tgggtctggt
     2701 ggtgggcgcg ctggcggtgg gcttctccgc ggccaccggc aagcccgccc aagagctgct
     2761 cttctcgggg cagtcggcgc tggggacgct gttccacgac agcgccggat attccatggc
     2821 ggcgctgctg ctgctcctcc tgggcaaggg cctggcgtac tgcgtgtcgc tgagcgcctt
     2881 ccggggcggg ccgatcttcc cggcgctgtt cctcggcgcg gccggcgggg tggtgctgtc
     2941 gcatctgccc gggctgtcgc tcaccccggc gttcgcgatg ggcgtcggag cgctggcggt
     3001 ggccatgctc cggctcccga tgacgtcggt gctgatggcg acgctgctga tgggccgcag
     3061 cggactcacg gtgatgccgc tggtgatcgt cgccgtcgtg gtcggctacg tggtgacgct
     3121 gcggctcacc ccggcacccg ccgcgcccgg ccgcaccgcc ccggcgccgt cggcggcctg
     3181 accgggcgtc cgttcagcgc ggggtgcggc cggtggccgt ccaggtggcc aactggcgct
     3241 ccgtccaggt gttgatgacc cgctccgcag gtacgccgca ctcctcggcg cgggcgcagc
     3301 cgtagatctg ccagtccagc tggcccgggg cgtgcgcgtc ggtgtcgatg gagaacagcg
     3361 tcccggccgc cagcgcccgg cgcagcagcc ggcgcggggg atcgaggcgc tcggggcggc
     3421 agttgatctc caccgcggtg cggtgctccg cgcaggccgc gaacacctcg tcggcgtcga
     3481 actccgactc cgggcggccc cggccggtca ccagccgccc ggtgcagtgc ccgaggacgt
     3541 ccaccagggg attgcgcacc gcggccacca tccgccgggt catcggcgcc ctgtccatcc
     3601 ggagcttgga gtgcaccgag gccaccacca cgtcgagccg ctccagcagc tccggttcct
     3661 ggtcgaggga gccgtcgtcg aggatgtcgc actcgatccc ggtcagcagg cggaacggcg
     3721 ccagccgctc gttcacctcc gccaccagct ccagctgttc ccgcagccgt tccgcggaca
     3781 gcccgcgggc gacggtgagc cggggggagt ggtcggtcag cacgcaccac tcgtgcccca
     3841 ggtcgcgggc ggcccgggcc atcacgtcga ccgggctgcc gccgtccgac cagtcggagt
     3901 gcagatggca gtcgccgcgc agggcccgcc gcagccgctg cccctgctcc ccctcgacca
     3961 gtggggcgcc ggcctcgtcc tccagccgtg ccagataggt gggccgcacg cccgccagcg
     4021 cctcttcgac cacccgggtg gtcttgggac ccagcccctt gaggcggacc agcgtgccct
     4081 gccgcgcccg ctcgcgcagc gcgtcgtccg acagcgcgcc gagcgcggcc gcggccccgc
     4141 ggaacgcctg tacgcggtag gtcgccgcgc cctgccgctc cagcaggaac gcgatccgct
     4201 ccagcgcgtc gacaggctcc acgtacggct ccttccgggt gcgccggtcc ccgcgggtcc
     4261 ctctcaccgt gccgcaaccg gacaccggct gccaccgggc ccgtcggccg ggccgacgcc
     4321 cgcgggcggt accggcggga aatcgcccat tcccgccgag tcggacggtg tgccgttatg
     4381 gggttatgtc cgttctcggg gcggtcgatc cgattccatg ggaatgccgt acggatagtg
     4441 ccgaagaatc cgtgcaaggc gttccatggg ctcgatacca ccccccgtaa ctccgggtaa
     4501 atcgggcgcc gttaaagctt gcgcaacatt cggcggaggg ttccccgggg gctcggcgga
     4561 gttctgcggg cgcccgtcga tcggtgctgt aaaaaccgcg tgtcttcaag gaatctgcac
     4621 accgattgcg cagccggcac accccctccc gctgtccgtc acggcgctga cctgcacgta
     4681 agcggtttga tgcgggaatg tcccgttcgc ctgacgggtc ttcgacaaca cgagcgaacg
     4741 caaaccgcag aggttgtgaa gaagctgcca ccaattcatg gacgtgcgtc cacgtcaggt
     4801 aattgagagg ttcgaagcgg tccggatcgg ccgtagtccg attagcagca caacccacct
     4861 gcatgctttg atccttcgca ctgcggaagt cgtgcagagg ttctcgattt cgagggcccg
     4921 ggcgaccgct gccgcggccg gaccgccctg cggacggctc ggtcgcagct atttgagata
     4981 tgcatccgaa aggaatcatc atgcgcgaga ttcagaccca ggaaatcgcc gacagcgagc
     5041 tggacaacgt ggccggtggc ctcgtcggca acctggccgg caccgccacc tccaccctcg
     5101 acggcgccac gggtctcaac gtcggcggca ccctcggcac cgtcaccggc accgtcgagg
     5161 gcctgaccgg cgtcaacacc ggccaggtca cgggcctcgt cgccggtctc taaagaccaa
     5221 cggcaggtcg gcgtcccctc cttgccgaca tgccggccgg atgccccgga accgccaggc
     5281 cttcgccggg ttccggggca cccggctgcc gagccccgga cccgacaccg tacgctcggc
     5341 cccaccgcgg cggccggaac gggcgccggg tcctggagtc ctgacagtcc tttgcagttg
     5401 agggaagagt gtcgtgcagt tccgccaaca ggccctttcc aagctgcaat cgcccgagga
     5461 aatcgatctg ccggtgcgct tcgcccggcc gcagggactg ctcgtcctga tcgtcacggt
     5521 cctctgcctg ctcgccgccg ccgtatgggc ggtgaccggg accgtctcct ccaccctcac
     5581 cgcacccggg atcctcaccc acggccaggg cagctacgtc ctgcagagcc ccgtcgcggg
     5641 acaggtcacc gccgtcctcg cccaagaagg ccagcgggtg tccgcgggcg ccccggtcct
     5701 caaggtccgc accccccggg gcaccgccgt cgtccgcacg atcgccgccg gccggctgac
     5761 cacgatggtc gccaccatcg gctcggtcgt caccaccggc gcggacgtgg cctccgtcga
     5821 acgcgtggca cacaccggcg accccctgac ggcgacgctg tacgtaccgg ccgagaacgg
     5881 cgcctcggtc cccgtcggcg cctccgtcga cctcaccgtc cagtccgtgc cgaaccagca
     5941 gtacggcgtg ctgcgcggcc gggtcaaggc cgtcggccgg accgcccaga cccgccagcg
     6001 gatcggcgcc tacctgggca gcagccagct cggcgagcag ttctccaaag ccggcccgcc
     6061 ggtcgccgtc ctggtacagc tcgaccgcgc cccccggaac aggtccggct acgcctggtc
     6121 cacctccgac ggcccgccct acaccgtcga ctccatgacg ctggccagcg gcgccgtcca
     6181 cctcgccgcg cagcacccga tcgattggct gctgccgtga ccgcatcgca ggactccgca
     6241 cccccgcagg accggctgcc gccggccggc cgcggccggc accgccccga agcggcgtcc
     6301 ggtgcccggc gccggcgcgc caccccgccc ccggcaccca agaaggtcaa ggcggtccgc
     6361 acccccaccg tcctccagat ggaggcggtg gaatgcggcg ccgcctccct ggcgatggtg
     6421 ctcgcccact acggacggca cgtccccctg gaggagctgc ggatcgcctg cggcgtctcc
     6481 cgcgacggct cccgcgccag caacctgctc aaggccgccc gcagttacgg cctgcaggcc
     6541 aagggcatgc agatggagac cgccgcgctc gccgaggtcc aggccccggc catcctcttc
     6601 tgggagttca accactacgt cgtctacgac ggcatgggcc gccggatggg ccgccgcggc
     6661 gtgcacatca acgaccccga caagggccgc cgcttcgtgc ccacggagga cttcgacacc
     6721 agcttcaccg gcgtcgccct ggtcttcgaa ccgtccgagt ccttccgcaa gggcggccgc
     6781 aagcccggcg tcctcggcgc ggtgcccgcc cggatgcgcg gcaccaccgg caccctgctc
     6841 gccgcgctcc tcgccagcct gctgctggtc gcggtcggcg ccgcggtgcc cgcgctcagc
     6901 cggacgtaca tcgacatgtt cctgatcggc aagcagacct cgctgctggg gccgctcttc
     6961 gcgtcgatgg ccgcgatggt cgcgctcacc gccgtcctca ccgggctgca acaggccaac
     7021 ctgctgcgcg gccggatcat ctcctccacc ctcaccagcg cccgcttcct gcgccatctg
     7081 ctccggctcc cggtcacctt cttctcccag cgcagcccgg ccgacctggt ccagcgcctg
     7141 cagtccaacg acgcggtcgc cgaaaccctc gcccgggacc tcgccgccgc cggcgtggac
     7201 ggcatcgtcg tcgtcctcta cgccgtgctg ctgtggacct acgaccccca gctcaccgtc
     7261 atcggcgtcg gcatcgccct gctcaacgtc gtcgccatgc ggatcgtgat ccggctgcgc
     7321 gccacccaca ccgccaaact ccgcgccgac accgcacggc tcaccaacac cgcctacacc
     7381 ggcctgcagc tcatcgagac catgaaggcc accggcgggg agaacggcta cttccgccgc
     7441 tgggccggcc agcacgccgc caccctggag gaacagcagc ggctcggcgt ccccagcgcc
     7501 tggctggccg tcgtcgcgcc caccctcgcc accctcaaca gcgccctgat cctctggatc
     7561 ggcggactgc gggccgtcga gggccacatc tccatcggtc tgctggtcgc cttccaggcg
     7621 ctggtcaccc gcttcaccgc gcccatcacc cggctcaacg gcgtcgccgg ccgcatccag
     7681 gacttcgccg ccgacgtggc ccgcctcaag gacgtcgagt ccttccccgc cgacagcctc
     7741 tacacccggc cgggctcgga cggcgacacc cgccggctca agggccacgt gacgctggag
     7801 aacatcacct tcggctacag cccgctggac aaaccgctgc tcaccggctt ctcactggcc
     7861 gtcggcccgg gccgccaggt cgccctggtc ggcggctccg gcagcggcaa gtccaccgtc
     7921 tcccggctga tatccggcct ctacagcccc tgggaaggca ccatccgcat cgacggacag
     7981 cgcctggagg acctctcccg cagcgcgctc gccgcctccg tctccttcgt cgaccaggac
     8041 gtcttcctct tcgagggaac ggtccgggac aacgtggccc tgtgggaccc ctccatcccc
     8101 gacgaggcgg tcatcaccgc cctcaaggac gccgccctct acgacgacgt catcgcccgc
     8161 cgccccgacg gcatccacag ccgggtcgac caggacggcc gcaacttctc cggcggccag
     8221 cggcagcgcc tggagatcgc ccgcgccctg gtccggcgcc ccagcatcct cgtcctcgac
     8281 gaggtcacca gcgccctcga cgcgcagacc gaacagatca tcatggacaa cctgcggcgg
     8341 cgcggctgcg cctgcgtcgt catcgcccac cggctcagca ccgtccgcga cagcgacgag
     8401 atcgtcgtcc tcgaccacgg cgtggtcgtc gaacgcggcc ggcacgagga cctggtcgcc
     8461 gccggaggcg cgtacgccga gctggtcaag gagcactgag gtgtcagccc tgccccccat
     8521 cccgcacgcg ggatccggcg agaccgacgc ggtgacgcag gccctgggcg gcctcggcgc
     8581 gccggtcgac tgcgccggac tgcgcagcgt ccccctcgaa gggccgcacg tgctgtggct
     8641 ggtcaccgcc ggcgcgctcg acctgttcgc ggtcgacgcc gcggcggagg gccactggca
     8701 cttcctgggc cggctcgaac ccggcgccct gctcctcggc ccggtcgacg gcccccagca
     8761 caccctgctc ggccgcccct cgcaggactg cgcggtgcgc cggatcccgc tgcgcgagct
     8821 gccccgttgg gactacggcg ggtacggcga gcagaccgac accggtccgc agcccctgta
     8881 cgccgcgccc ggcggctacg gccaccccgc ggcgtacggc ccgctggagc acgccttcgc
     8941 gctgggcacc gcccgcagcc tcggcgtgct cttcgaggcc ccgctggacg gccggcccgg
     9001 cgacgaggcg gtggccgacg acgacgtcct ctggatgccg gtcccgcccg gcagcgtcca
     9061 gtacggcgcc tcctacagcg cggaggcggc cgccgacctg ctcgtcgacc ccgaactgtg
     9121 gcagcggatg gtcaaccagc agtaccggct gctgtccgcc gtcgaccgct ggatcgagca
     9181 gctggagcgc gcccacgagg accgtacggc ggccggcatc aaggccggtg aggccgtccg
     9241 cgagcgcgcc gaccaggcgc tgctggcctc catcggccgg caggaccgcg ccgggcgggg
     9301 cggcgccggg acggaccggg gcgacgacac caccttcgcg gtctgccgca cggtcgccga
     9361 ggccgccggg atcaccctca ccgagccccc caggggcggc gccgtcaacg accgcatcac
     9421 ccccgtcgaa cggatcgcgg tcagctcccg gatccgcacc cgcgcggtcc ggctcaccgg
     9481 cacctggtgg cggaccgaca ccggccccct ggtcggccac cgcgccaagt ccggcgcccc
     9541 cgtcgcactg ctgtggcgcc gcggccgcta cgaggcggtc aaccccgcct ccggcctgcg
     9601 gatgcggatc ggcaaggaca acgccgacga gttcgaggcc cgcgccgtca tgttctaccg
     9661 cccgctgccc gagcggccga tgaacatgtg gcggctgatg cgcttcagcc tgcgcggcac
     9721 ccgcaccgac gtccgcaacc tcgccctgag cggactggtg acggtcgccc tcggcgcgct
     9781 cgtcccgatc gcgaccggca aggtcctcgg ggtgtacgtc ccggccgccg acaagagcct
     9841 gatcgtgcag gtcgcactgg ccgtcatcgt cagcagcgtg gtcgccgccg ccttcatgct
     9901 gctgcagagc ctcaccgtgc tccgcatgga gggccgaata gagagcgccc tgcaaccggc
     9961 cgtctgggac cggctgctgc ggctgccgac caagttcttc accgaacgct ccaccggcga
    10021 actggccagc gccgcgatgg gcatcagcgc catccggcgg gtgctgtccg gcatcggccc
    10081 ggtggccgtg cagtccacga ccgtcggcgc gatgaacctc gtcctgctcc ttgtctacag
    10141 cgtgccgctg gcgctcgccg cgctcggcat gctggtcgtc atcggggcgg tcttcctcat
    10201 catgggcctg tgg
//
