LOCUS       NZ_AP026073            10417 bp    DNA     linear   CON 31-DEC-2024
DEFINITION  Streptomyces nigrescens strain HEK616 chromosome.
ACCESSION   NZ_AP026073
VERSION     NZ_AP026073.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces nigrescens
  ORGANISM  Streptomyces nigrescens
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Complete genome sequence of Streptomyces nigrescens HEK616
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8253039)
  AUTHORS   Asamizu,S. and Onaka,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2022) Contact:Shumpei Asamizu The University of
            Tokyo, Graduate School of Agricultural and Life Sciences; Yayoi
            1-1-1, Bunkyo, Tokyo 113-8657, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP4 v. 4.0; Falcon v. 0.3; Canu v. 0.7; Arrow
            v. 2.3.3; Pilon v. 1.22
            Genome Coverage                   :: 262x
            Sequencing Technology             :: illumina Hiseq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024349765.1-RS_2024_12_31
            Annotation Date                   :: 12/31/2024 04:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,326
            CDSs (total)                      :: 8,236
            Genes (coding)                    :: 8,032
            CDSs (with protein)               :: 8,032
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 84 of 204
            Pseudo Genes (incomplete)         :: 166 of 204
            Pseudo Genes (internal stop)      :: 16 of 204
            Pseudo Genes (multiple problems)  :: 56 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:47:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1822673
            Orig. end                         :: 1833090
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP026073.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(96..809)
                     /locus_tag="HEK616_RS07815"
                     /old_locus_tag="HEK616_16060"
     CDS             complement(96..809)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091833.1"
                     /locus_tag="HEK616_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16060"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_261952173.1"
                     /transl_table=11
                     /translation="MTSPQGFLGFWETTGSAKTFTHPLDPALLDAYVPRRARVLDYGCG
                     YGRLTAELAGLGYRGVCGVDVSAALIARGRREHPELDLRRHAGLPLPFEAGSFDAALLF
                     AVLTCVPTDVDQEAITGELGRLVRPGGVLYVSDVPLQEDAFNQERYRRFAARYGTFGVF
                     ETPDGGVFRHHAPDRLRGLVRAAGFSVVAERSGSVGTLDGRRAERLQLIARRTADPAAA
                     AADRPEPGRSGPAPE"
     gene            889..1593
                     /locus_tag="HEK616_RS07820"
                     /old_locus_tag="HEK616_16070"
     CDS             889..1593
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="HEK616_RS07820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16070"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_261952174.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHRDEVEVVHRSFELDP
                     KAPPASDVPVLDMLAAKYGVSRAQAEQMEARVAAAAAQEGLGYRSDRIHGNTFDLHRLL
                     HLGAAHGRQEALLDAFYRANFAEARPLGDPAVLAELATGAGLPADEVARVLADPDAYAR
                     EVRADERAAAELGATGVPFFVIDRRYGISGGQPAEVFREALERAHADGVVEIVPAGDAE
                     VCGPDGCAVPGH"
     gene            1676..2746
                     /locus_tag="HEK616_RS07825"
                     /old_locus_tag="HEK616_16080"
     CDS             1676..2746
                     /NRPS_PKS="Domain: Aminotran_5 (16-350). E-value: 4.2e-20.
                     Score: 63.9. Matches aSDomain:
                     nrpspksdomains_HEK616_RS07825_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606338.1"
                     /locus_tag="HEK616_RS07825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16080"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_261952175.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-13, bitscore:
                     49.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHEPLGGAEFAPETTYLNSASSGLLPRRSADALRAAVEEATSY
                     GTMGRDYFAAPAAARAAFARLLGIPAERVAIGSSVAVQSAAVAASLPPGSEVLAPEGDF
                     SSLVNPLGTHPDLTLRTVPLEALADEVRPGTALVAFSAVQSLDGRIADLAAVREAAAAH
                     GALTYLDATQAAGWLPLAATDYDFLVCGAFKWLLCPRGTTFMAFGGDRGPDGTGPAWPA
                     APHAGWVAGEDIGESNYGLIRPAATARRYDEPHAHFAYVGAAASLGLIEELGVAAVHAH
                     NTALADQYRAGLAERGLTPRGAPGSAIVSAPGPADAQERLAEAGVRISVRGGLLRAAFH
                     LYNGTADVERLLELLK"
     aSDomain        1724..2725
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS07825_Aminotran_5.1"
                     /evalue="4.20E-20"
                     /label="HEK616_RS07825_Aminotran_5.1"
                     /locus_tag="HEK616_RS07825"
                     /protein_end="350"
                     /protein_start="16"
                     /score="63.9"
                     /tool="antismash"
                     /translation="TYLNSASSGLLPRRSADALRAAVEEATSYGTMGRDYFAAPAAARA
                     AFARLLGIPAERVAIGSSVAVQSAAVAASLPPGSEVLAPEGDFSSLVNPLGTHPDLTLR
                     TVPLEALADEVRPGTALVAFSAVQSLDGRIADLAAVREAAAAHGALTYLDATQAAGWLP
                     LAATDYDFLVCGAFKWLLCPRGTTFMAFGGDRGPDGTGPAWPAAPHAGWVAGEDIGESN
                     YGLIRPAATARRYDEPHAHFAYVGAAASLGLIEELGVAAVHAHNTALADQYRAGLAERG
                     LTPRGAPGSAIVSAPGPADAQERLAEAGVRISVRGGLLRAAFHLYNGTADVER"
     gene            2848..3915
                     /locus_tag="HEK616_RS07830"
                     /old_locus_tag="HEK616_16090"
     CDS             2848..3915
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045205.1"
                     /locus_tag="HEK616_RS07830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16090"
                     /product="alkene reductase"
                     /protein_id="WP_261952176.1"
                     /transl_table=11
                     /translation="MTTAFEPFELAGIPLANRIVMAPMTRNRATEEGVPTPSMVRYYTQ
                     RASAGLIITEAAQTEPVGRGYPLTPGLHTPEQLAGWRAVTDSVHAAGGRIFAQLWHGGR
                     IGHPVLLPDGLVPVGPSAVAAAGQGFTQEGPTDFVTPRELTGEEIAATIAGFATAARNA
                     VEAGFDGVEIHGANGYLIHQFLAPNANLRTDEWGGSDEARARFAIEVVRAVADAVGAHR
                     TGLRLSPGNPFNDIAEPAPETTYTTLIEAIAPLGLAYLHTCDVRDPDLLARLRKRFGGT
                     FLLNPATDGRPTGPEELGLVEEGEADLLAYGALFLANPDLPRRLALGGPFNTPDRASFY
                     GGDDTGYLDYPALEG"
     gene            complement(4005..4904)
                     /gene="thpD"
                     /locus_tag="HEK616_RS07835"
                     /old_locus_tag="HEK616_16100"
     CDS             complement(4005..4904)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="HEK616_RS07835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16100"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_261952177.1"
                     /sec_met_domain="PhyH (E-value: 2.7e-37, bitscore: 127.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTSEVITPRKDPVVWSQPGTAGPFAPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPTMRADPRSIVEPKSQSVRSVFEVHKI
                     SEVFAKLVADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHQHFVGCEGETPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHGIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFVVFNSVDNTAVEPFA
                     APVRRPDYIGARDFTPVR"
     gene            complement(5001..5417)
                     /locus_tag="HEK616_RS07840"
                     /old_locus_tag="HEK616_16110"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="HEK616_RS07840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16110"
                     /product="ectoine synthase"
                     /protein_id="WP_261952178.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-52, bitscore:
                     175.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTNDETGEKHLITPGVMYLLDGHEKHTMRIKQDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPDPEPETV"
     gene            complement(5472..6740)
                     /gene="ectB"
                     /locus_tag="HEK616_RS07845"
                     /old_locus_tag="HEK616_16120"
     CDS             complement(5472..6740)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.3e-74.
                     Score: 243.2. Matches aSDomain:
                     nrpspksdomains_HEK616_RS07845_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="HEK616_RS07845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16120"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_261952179.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-76, bitscore:
                     256.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRITDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGQTPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEAHLK
                     AIVEENPGAFAEYRGRGLVWGLECNDKPLAGKIAARAFELGLLIETSGPQSEVVKLLPA
                     LTTTPEELDEGLRILGRAVRDCA"
     aSDomain        complement(5646..6656)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HEK616_RS07845_Aminotran_3.1"
                     /evalue="1.30E-74"
                     /label="HEK616_RS07845_Aminotran_3.1"
                     /locus_tag="HEK616_RS07845"
                     /protein_end="365"
                     /protein_start="28"
                     /score="243.2"
                     /tool="antismash"
                     /translation="FDRAQGSRITDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGQTPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAALCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEIVEAHLKAIVEENPGAFAEYRGRGLVWGLECN"
     gene            complement(6869..7435)
                     /gene="ectA"
                     /locus_tag="HEK616_RS07850"
                     /old_locus_tag="HEK616_16130"
     CDS             complement(6869..7435)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059805.1"
                     /locus_tag="HEK616_RS07850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16130"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_261952180.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIGEFPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDAEGEPAAFLTGYIRPERPETLVVWQVAVDAAHRGR
                     GLAAALLDGVTTRAREELGIRFVETTITPDNAASNRLFASFGERHSVPVEREVLFDAGL
                     FPEQGHEPEVLHLIGPFAPPARPAP"
     misc_feature    complement(7435..7449)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.04"
                     /tool="antismash"
     misc_feature    7445..7460
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    complement(7591..7606)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7601..7616
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7793..7806)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7825..7838
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.2"
                     /tool="antismash"
     misc_feature    complement(7825..7839)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.1"
                     /tool="antismash"
     misc_feature    complement(7826..7839)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.84"
                     /tool="antismash"
     misc_feature    complement(7827..7840)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.94"
                     /tool="antismash"
     gene            8013..9104
                     /locus_tag="HEK616_RS07855"
                     /old_locus_tag="HEK616_16140"
     CDS             8013..9104
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="HEK616_RS07855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16140"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_261957179.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.2e-16, bitscore:
                     58.2, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWVVGGRVTYDRPTLARDAISV
                     QGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTDRDAGTLLIRDAGSPSDTRWVDDRA
                     DLPKIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LGACWPKGAVEAAIAEAHRLGARVTAHCFAEETLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPRLADAGAGKFPRWADHMRRLHDRRYDTVRAAHDAGVPVFAG
                     TDAGGSLAHGLVADEVVELTRAGLPARAALSATTWGAREWLGRPGLTEGAPADLVVYES
                     DPREDVRVLAAPRRVVLRGQIVG"
     gene            complement(9126..10121)
                     /locus_tag="HEK616_RS07860"
                     /old_locus_tag="HEK616_16150"
     CDS             complement(9126..10121)
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142648.1"
                     /locus_tag="HEK616_RS07860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HEK616_16150"
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_410011167.1"
                     /transl_table=11
                     /translation="MSIEQNSQHPDAQREQPAAAPPGELPASDRAARRAVTVFPLLVVA
                     AGAVGLLWPAHFTGWAPAVPWLLGVVMFTMGLTLTPPDFTAVARRPWAVGLGLVAHYVI
                     MPGLGWLIATVLDLPPQLAAGVILVGCAPSGTASNVVTFLARGDVALSVSVATVSTVLA
                     PLITPALTLLLAGQFLAVDAGGMVTDILKTVLLPVVAGLLVRLLAGRFVDRLLRALPWL
                     SAATVAVIVLVVVSGSAPAIKAAAVPVLLAVVLHNGLGLALGHAAGRLARLGGPASRAM
                     AFEVGMQNSGLAASLATAHFSPAAALPAAVFSVWHNVSGAVVAAWMARRR"
     misc_feature    complement(10292..10305)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 cgtcctctgg cacaaccacc gcgcccgcgc cttctacgac cggctcggct ggcgccccga
       61 cccggaccgc cgccccgcgc ccgacgccac ccacctcact ctggcgctgg acctgaccgg
      121 cccggctccg gacggtccgc agcggcggcc gccggatccg ccgtcctccg ggcgatgagc
      181 tgcagccgct cggcccgccg cccgtccagc gtccccaccg acccggaccg ctccgccacc
      241 accgagaagc ccgccgcccg caccagcccg cgcagccgat ccggggcgtg gtgccggaag
      301 accccgccgt cgggcgtctc gaacaccccg aacgtgccgt accgggccgc gaaccgccgg
      361 taccgctcct ggttgaacgc gtcctcctgc agcggcacat cgctcacgta cagcaccccg
      421 cccggccgca ccagccgccc cagctccccg gtgatcgcct cctggtccac gtctgtgggc
      481 acacaggtga gcaccgcgaa cagcagcgcc gcgtcgaagg acccggcttc gaacggcagc
      541 ggcagcccgg cgtgccgccg caggtccagc tccgggtgct cccgccgccc ccgcgcgatc
      601 agcgccgccg agacgtccac cccgcacacg ccccggtacc ccagccccgc cagctcggcg
      661 gtcagccgcc cgtagccgca gccgtagtcc agcacccgcg cccgacgcgg tacgtaggcg
      721 tccagcaggg ccggatcgag cgggtgggtg aaggtcttcg cggagccggt cgtctcccag
      781 aacccgagga atccctgtgg cgaggtcatg tccggacggt acccgcggaa tagcccggcg
      841 ccccccgcgg ttgcacgcag cacacgtccc gacgaggaaa gtcagcacat gcgcgtcgaa
      901 atctggtccg acatcgcctg cccctggtgc tacatcggca aggcccgctt cgaggccggg
      961 ctcgccgcgt tcgcgcaccg cgacgaggtc gaggtggtgc accgctcctt cgagctggac
     1021 ccgaaggcgc cgcccgcctc ggacgtcccg gtgctcgaca tgctcgccgc caagtacggc
     1081 gtcagccgcg cccaggccga gcagatggag gcccgggtcg ccgccgcggc cgcccaggag
     1141 ggcctcggct accgctccga ccgcatccac ggcaacacct tcgacctgca ccgcctgctg
     1201 cacctcggcg cggcgcacgg ccgccaggag gcgctgctcg acgccttcta ccgcgccaac
     1261 ttcgccgagg cccggccgct cggcgacccc gccgtcctcg ccgagctcgc caccggggcc
     1321 ggactgcccg ccgacgaggt cgcccgggtg ctcgccgacc ccgacgcgta cgcccgggag
     1381 gtgcgcgccg acgagcgcgc ggcggccgag ctcggcgcca ccggcgtccc gttcttcgtc
     1441 atcgaccgcc gctacggcat atcgggcggc cagcccgccg aggtcttccg ggaggccctg
     1501 gagcgggcgc acgccgacgg tgtggtggag atcgtgccgg ccggcgacgc ggaggtctgc
     1561 gggccggacg gctgcgcggt gcccggccac tgaccgccgg ccggcggccg accgggcgcg
     1621 accacggacc ggaacaccgt ggacggagcg tgccccgccg agcacagtgg gggacatgca
     1681 gcgacatgag ccactcggcg gggccgaatt cgcgcccgag acgacgtatc tgaactccgc
     1741 ctccagcgga ctgcttccac ggcgcagcgc cgacgccctg cgcgcggccg tcgaggaagc
     1801 cacctcctac ggcacgatgg gccgcgacta cttcgccgcg ccggccgccg cccgggccgc
     1861 cttcgcccgg ctgctgggca tccccgccga gcgcgtcgcc atcggcagct cggtcgcggt
     1921 gcagtccgcg gccgtcgccg cctcgctccc gcccggcagc gaagtcctcg cccccgaggg
     1981 cgacttcagc tccctggtca acccgctcgg cacccacccc gacctcaccc tgcgcaccgt
     2041 tccgctggag gcactggccg acgaggtccg ccccggcacg gccctggtcg ccttcagcgc
     2101 cgtgcagtcg ctcgacggcc ggatcgccga cctcgcggcg gtgcgcgagg ccgcggcggc
     2161 ccacggggcg ctcacctacc tcgacgccac ccaggccgcc ggctggctgc cgctggccgc
     2221 caccgactac gacttcctgg tctgcggcgc gttcaaatgg ctgctctgcc cgcgcggcac
     2281 gaccttcatg gccttcggcg gcgaccgcgg cccggacggc accggccccg cctggccggc
     2341 ggcgccgcac gccggctggg tggccggcga ggacatcggc gagtccaact acggcctgat
     2401 ccggccggcc gccaccgccc gccgctacga cgaaccgcac gcccacttcg cgtacgtcgg
     2461 cgccgcggcg tccctgggcc tcatcgagga gctgggcgtg gccgccgtcc acgcccacaa
     2521 caccgcgctc gccgaccagt accgcgccgg gctggccgaa cgcggcctga ccccgcgcgg
     2581 cgcgcccggc tcggcgatcg tctccgcccc cggaccggcc gacgcgcagg agcggctggc
     2641 cgaggccggc gtgcggatct cggtgcgcgg cggcctgctg cgcgcggcct tccacctcta
     2701 caacgggacg gccgacgtgg aacggctgct ggagctgctg aaatagccgg ccggtgacgg
     2761 gaatgccggg cgggcccggg gcgttggaag gtgacgaacc gtcatgggca atgtccctgc
     2821 cgtcgcaccg tagttgagag gccctcagtg accaccgcat tcgagccctt cgagctcgcc
     2881 ggcattccgc tcgccaaccg catcgtgatg gcgcccatga cgagaaaccg cgccaccgag
     2941 gagggcgtcc ccaccccgtc gatggtccgc tactacaccc agcgggcctc ggccggcctc
     3001 atcatcaccg aggccgcgca gaccgaaccg gtcggccgcg gctacccgtt gacccccggc
     3061 ctgcacaccc ccgagcagct cgccggctgg cgcgcggtca ccgactccgt gcacgccgcg
     3121 ggcggcagga tcttcgccca gctgtggcac ggcggccgca tcggccaccc ggtgctgctg
     3181 ccggacggcc tggtgccggt cgggccctcc gcggtcgccg cggccggcca gggcttcacc
     3241 caggagggcc cgacggactt cgtcaccccg cgcgagctga ccggcgagga gatagccgcg
     3301 acgatcgccg gattcgccac ggcggcccgc aacgccgtcg aggccgggtt cgacggcgtg
     3361 gagatccacg gcgccaacgg ctatctgatc caccagttcc tggcgcccaa cgccaacctc
     3421 cgcaccgacg agtggggcgg ctccgacgag gcccgggccc gcttcgccat cgaggtcgtc
     3481 cgggccgtcg cagacgccgt cggcgcgcac cgcaccggac tgcggctctc gcccggcaac
     3541 cccttcaacg acatcgccga gcccgccccc gagaccacct acaccacgct gatcgaggcg
     3601 atcgccccgc tgggcctggc ctatctgcac acctgcgacg tccgcgaccc ggacctgctg
     3661 gcccggctgc gcaagcgctt cggcggcacg ttcctgctca accccgccac cgacggccgg
     3721 cccaccggcc ccgaggagct cgggctcgtc gaggagggcg aggccgatct gctcgcctac
     3781 ggcgcgctgt tcctcgccaa ccccgacctg ccccgccggc tcgccctcgg cggccccttc
     3841 aacacccccg accgggcgag cttctacggc ggcgacgaca ccggctacct cgactacccg
     3901 gcgctggagg gctgacggag gaccgaacca cagcgcggcc gccgcacaga ccctgctgtg
     3961 cggcggccgc gccaccggta cttccgccgg tctacctcat acggctatct caccggggtg
     4021 aagtcccgcg cgccgatgta gtccgggcgc cggaccgggg ccgcgaaggg ctccacggcc
     4081 gtgttgtcca cgctgttgaa gacgacgaag acgttgctgc gcgggtacgg cgtgatgttg
     4141 tcgccggagc cgtgcatggc gttgcagtcg aaccacgtcg ccgagccggc cttgccggtg
     4201 aagaggcgga tgccgtgctt gtccgccatc ttcgtcagca cctcgtccga cgggatgccc
     4261 gcgtcctgca tctgcagcga ccgcttgtag ttgtccttcg gcgtctcgcc ctcgcacccc
     4321 acgaagtgct ggtgcgaacc gggcatgatc atcaggccgc cgttggtgtc gtagttctcc
     4381 gtcagcgcga tcgagaccga cacggtgcgc atgttcggca gcccgtcctc ggcgtgccag
     4441 gtctcgaaat ccgagtgcca gtagaagccg gaggcaccga agccgggctt cacgttgatc
     4501 cgcgactggt ggacgtagac gtccgagccg aggatctggc gggcacggcc gaccacacgc
     4561 gggtcggcga ccagcttggc gaacacctcg ctgatcttgt gcacctcgaa caccgaccgg
     4621 acgctctgcg acttcggctc gacgatggag cgcgggtcgg cgcgcatcgt cgggtcgagc
     4681 accagccggt ccagttcggc gcggtacacc gcgacttcct ccgcggtgag cagttccccg
     4741 atggcgaaga agccgtcgcg ttcgaagtcg ctcagctcgg acggcgcgaa cgggccggcc
     4801 gttccgggct gcgaccacac caccgggtcc ttccgcggag tgatcacctc ggaggtgccg
     4861 cgggtcgggt acaggtcggc ggtgcgctcg ggtgcggtgg tcatggtgtt gccttcctct
     4921 cctctcgtac ggaatctggc atacgggcgc gcgttgcgta ccttcctggc tctgccggac
     4981 ggggcaggcg tcaggtcgcc tcagaccgtc tcgggctcgg ggtccggttc ggtgagaagc
     5041 gggtagaccc cgttctcgtc gtggtcctcg cggccggtga ccggcgggtt gaagacacac
     5101 aggcagcgga agtcctgctt gatccgcatg gtgtgcttct cgtgcccgtc gagcaggtac
     5161 atcacgccgg gcgtgatgag gtgcttctcg ccggtctcgt cgttggtcag ctcggcctcg
     5221 ccctcgacgc agacgacggc ctcgatgtgg ttggcgtacc acatcgacgt ctcggtgccg
     5281 gcgtacagga cggtctcgtg cagcgagaag ccgacccgct ccttggcgag cacgatgcgc
     5341 ttgctctccc aggtgccgga cttggacttg acgtggcggt cggtgccttc gatgtccttg
     5401 aaggagcgga cgatcacggt gcgggctgcc tttctgtcgt atctgtggtg cggtgcggtg
     5461 cggaacgccg gtcaggcgca gtcgcggacc gcgcggccga ggatgcgcag accctcgtcc
     5521 agttcctcgg gtgtggtggt cagggccggc agcagcttga cgacctcgct ctgcggaccg
     5581 gaggtctcga tcagcaggcc cagttcgaag gcgcgcgcgg cgatcttccc cgcgagcggc
     5641 ttgtcgttgc actccaggcc ccacaccaga ccgcggccgc ggtactcggc gaaggcgccg
     5701 gggttctcct cgacgatcgc cttcaggtgc gcttcgacga tctcaccgcg ggccagggtc
     5761 tgcttctcca tctgcccgtc ggcccagtag gtgtcgagcg ccgcggcggc cgtgacgaac
     5821 gccgggttgt tgccgcggaa ggtgccgttg tgctcgcccg gctcccagat atccagctcc
     5881 ggcttgaaca gggtgagcgc gagcggcagg ccgtagccgc tgatggactt cgagaccgtg
     5941 acgatgtccg gcacgatgcc cgcctcctcg aaggagaaga aggcacccgt acggccgcag
     6001 cccatctgga tgtcgtcgac gatcagcagc atgtcctggc gctcgcacag ggcggccagg
     6061 gcgcgcagcc actcggcgcg ggcgacgttg atgccgccct cgccctgcac ggtctccacg
     6121 atcaccgcgg ccggcttgtt caggccggag ccctggtcct ccagcagccg ctcgaaccac
     6181 aggaagtccg gggtctggcc gtcgaggtag ttgtcgaacg gcatcggggt gccgtgcacc
     6241 agcgggatgc cggcgccggc ccgcttcatg gagttgccgg tgaccgcgag ggcgcccagc
     6301 gacatgccgt ggaaggcgtt ggtgaaggac acgatcgact cgcggccctt gaccttacgg
     6361 gccagcttca gcgcggcctc gaccgagttg gcgcccgtcg gacccgggaa catgaccttg
     6421 tagggcaggt cgcgcggccg caggatgatg ttctggaacg actccaggaa cgcccgcttg
     6481 gccgtggtgg acatgtccag gccgtgggtg atgccgtccc gctcgatgta gtcgatcagg
     6541 gcgcgtttga ggaccgggtt gttgtgcccg tagttgagcg acccggcgcc ggcgaagaag
     6601 tcgaggtagg tgtggccgtc ctcgtcggtg atgcggctgc cctgcgcgcg gtcgaagacg
     6661 gtgggccagc cacggcagta gctgcgcacc tccgactcca cggtctcgaa gacgctcaga
     6721 tcgggctggg tgatggtcac agcatgctcc tgggagatga gtgcgagagg cggaagaagt
     6781 ctgttggggg gctgggcggc gccggcggtt cgagggggtg ccggtgccgc acggccgtcg
     6841 cggcggcgct gccgcgcggc cctgggggct agggcgcggg ccgggccggg ggcgcgaacg
     6901 ggccgatgag gtgcagcacc tccggctcgt ggccctgctc ggggaacagc ccggcgtcga
     6961 agagcacctc gcgctcgacc ggcacggagt gccgctcgcc gaaggacgcg aacagccggt
     7021 tggaggcggc gttgtcgggg gtgatggtgg tttcgacgaa acggatgccc agctcttccc
     7081 gggcacgggt ggtcaccccg tccaggaggg cggccgccag cccccggccc cggtgcgccg
     7141 catcgacggc gacctgccag acgacgaggg tctcggggcg ttcgggacgg atgtagccgg
     7201 tcaggaaggc ggccggctcg ccctccgcgt cgcgcgcgac gacggaggtg gcggcgaagt
     7261 cccgacacca caggaggtag ctgtacgagg agttcaggtc cagcgccttg gagtcgcggg
     7321 cgatgcgcca gatcgcggct ccgtcctcca cgcggggagt gtcgagcttg aagccctccg
     7381 gaaattctcc gatatcgcta cgggcacctg catggtctgc ttgtgcggcg gtcatggaaa
     7441 ttaaatttac ccagggaaaa aggaaattgc atcgcgggca ggggttacgt cgggccgtct
     7501 gatgtgttat cgcgcggggg cgcccgggcg cgcgacgtcg aggcgggttg cccccatttg
     7561 accgggaagt acggggcgaa acggacgggc tgtggagtcg gtcacaggtc cgtaacgccc
     7621 gcgagatccg tccgaattac gggcttcgac gctagcggaa tcttggcgtt tgagctgcgg
     7681 gaaagcgggc agaagaagac gggaagctgt actcgaagaa attgggaatt cgccaccgga
     7741 gaccctggcg caaggccccg gaaaagctgt gcggcacatc catgagaagc accgcaagaa
     7801 catgcggcaa gaacgcgcgc ggggcttttt cggaaacatt gcggcaataa acgcccacac
     7861 agcgtcggcg ccgtgccggc gagcgtcccg gaggggccgt ccgcaccgcg gtcaccggcc
     7921 cgtcggcccc cgtccccggc cgcccgtccg gcgctgtgac cgccgtctcc ggcgagggcg
     7981 ccggcgccgc ctagagtgcc ggtatgacgt ccatgagtga aggtgcggtg ctgcacctca
     8041 aggggagggt cctcgtcgga ccggacgacg tgcgcgacga gctgtgggtg gtcggcgggc
     8101 gggtgaccta cgaccggccg accctggcac gcgatgccat cagcgtgcag ggctgggcgc
     8161 tgcccggcct ggtcgacgcg cactgccacg tggggctgga cgcgcacggc ccggtggacg
     8221 cccccaccag cgagaagcag gcgctcaccg accgtgacgc cggcaccctg ctgatccggg
     8281 acgccggatc gccctccgac acccgctggg tcgacgaccg cgcggacctg ccgaagatca
     8341 tccgggccgg ccggcacatc gcccgcacca agcggtacat ccgcaactac gcccatgaga
     8401 tcgagcccga ggacctggtc gcctacgtcg cccaggaggc ccgccgcggc gacggctggg
     8461 tcaagctcgt cggcgactgg atcgaccgct ccaccgggga cctgggggcc tgctggccca
     8521 agggcgcggt cgaggcggcc atcgcggagg cgcaccgcct gggcgcccgg gtgaccgcgc
     8581 actgcttcgc cgaggagacc ctcgccccgc tcgtcgaggc cggcatcgac tgcatcgagc
     8641 acgccaccgg gctcaccgag gagaccatcc cgctgttcgc cgagcgcggg gtcgccatcg
     8701 tccccaccct cgtcaacatc gccaccttcc cgcgcctggc ggacgccggg gccgggaagt
     8761 tcccgcgctg ggcggaccac atgcgccggc tgcacgaccg ccgctacgac accgtccggg
     8821 cggcccacga cgccggggtg cccgtcttcg ccggcaccga cgcgggcggc tcgctggcgc
     8881 acggcctggt cgccgacgag gtcgtcgagc tgacccgggc cgggctgccg gcccgcgccg
     8941 cgctctcggc caccacctgg ggcgcccgcg agtggctcgg ccgcccgggc ctcaccgagg
     9001 gcgcgccggc ggacctggtc gtctacgaga gcgatccgcg cgaggacgtc cgggtgctgg
     9061 cggcgccgcg ccgggtggtg ctgcgcgggc agatcgtggg ctgaggcacc ccctcgggcc
     9121 gggagctacc tccggcgggc catccaggcc gccaccaccg cgcccgagac gttgtgccac
     9181 accgagaaga ccgccgcggg cagcgccgcg gccgggctga agtgggcggt ggccagcgag
     9241 gcggccagtc cggagttctg catgccgacc tcgaaggcca tcgcccggct cgccggcccg
     9301 cccagccggg ccaggcggcc cgcggcgtgc ccgagcgcca gcccgaggcc gttgtgcagc
     9361 accacggcca gcagcaccgg caccgccgcc gccttgatcg ccggcgcgct gccggagacc
     9421 acgaccagga cgatcaccgc gacggtcgcc gcggacagcc agggcagcgc ccgcagcaga
     9481 cggtccacga accggcccgc gagcagccgg accagcagac cggccacgac cggcagcagc
     9541 accgtcttca ggatgtcggt caccatgccc ccggcgtcca cggccaggaa ctgcccggcg
     9601 agcagcagcg tcagcgccgg cgtgatcagc ggcgcgagca ccgtcgagac cgtcgccacc
     9661 gacacggaca gcgcgacgtc gccgcgggcc aggaacgtca cgacgttgga cgcggtcccg
     9721 ctgggcgcgc agccgacgag gatcaccccg gccgcgagct gcggcggcag gtccagcacg
     9781 gtcgcgatca gccagccgag ccccggcatg atcacgtagt gggcgaccag gcccagcccc
     9841 accgcccagg gccgccgggc gaccgccgtg aagtccggcg gggtcagcgt cagccccatc
     9901 gtgaacatca ccacgccgag cagccacggc accgcgggcg cccagccggt gaagtgcgcc
     9961 ggccacagca gtccgaccgc ccccgcggcg accaccagca gcgggaagac ggtgaccgct
    10021 cggcgggcgg cccggtcgga ggcgggcagc tcgcccggag gcgccgcggc cggttgttcc
    10081 cgctgtgcgt ccgggtgctg gctgttctgc tcgatcgaca cggcggcacc ctatccgggg
    10141 tcttcgtgta caggacaacg agttccggat cctggacgcg gccacgatcc gggcacggcc
    10201 gggcgcgggc cggtcaaccg ggatcacccg gggggtgggg cggttgtggc gagggaggtg
    10261 cgggatgtcg cgggaatcga acccacgggc ggaaaccacc ctttgggatg aactgacctc
    10321 acgtggctgc ccgttcaccc tcggtgccga cacgatgtgc gggtcgatgt cgccggcccg
    10381 cgtgatgtcc cccattggcg cggccggcgg ctccatt
//
