LOCUS       NZ_JANAVN010000033     10372 bp    DNA     linear   CON 26-JUL-2024
DEFINITION  Paenibacillus sp. Lou8.1 NODE_33_length_10372_cov_148.123944, whole
            genome shotgun sequence.
ACCESSION   NZ_JANAVN010000033
VERSION     NZ_JANAVN010000033.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. Lou8.1
  ORGANISM  Paenibacillus sp. Lou8.1
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Gonzalez-Pimentel,J.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2022) Agroquimica, Microbiologia Ambiental y
            Conservacion de Suelos, Instituto de Recursos Naturales y
            Agrobiologia, Av. Reina Mercedes 10, Sevilla 41012, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: SPAdes v. 3.14.1
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024213565.1-RS_2024_07_26
            Annotation Date                   :: 07/26/2024 02:53:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,255
            Genes (coding)                    :: 5,159
            CDSs (with protein)               :: 5,159
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 74 of 96
            Pseudo Genes (internal stop)      :: 9 of 96
            Pseudo Genes (multiple problems)  :: 25 of 96
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:00:54
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10372
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JANAVN010000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..4503)
                     /locus_tag="NLX78_RS26270"
                     /old_locus_tag="NLX78_26255"
     CDS             complement(<1..4503)
                     /NRPS_PKS="Domain: Condensation_Starter (8-297). E-value:
                     1.9e-75. Score: 246.0. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26270_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (464-898). E-value:
                     6.2e-113. Score: 369.4. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26270_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1005-1072). E-value: 2.2e-24.
                     Score: 77.4. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26270_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (1083-1391). E-value:
                     5.3e-151. Score: 495.9. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26270_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1395-1500). E-value: 2.3e-35.
                     Score: 113.7. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26270_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310169.1"
                     /locus_tag="NLX78_RS26270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NLX78_26255"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_254351914.1"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-121, bitscore:
                     393.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5e-15, bitscore:
                     44.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2e-80, bitscore:
                     258.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQQDVQLFSLSQPQQRIWYTELLYPNRNTSTIIATVKIRGTVRI
                     DALQQAMNRVIAQNDSFRIKITAQDGIPYQYVEPFTAESIETLQVTPEEAKEWLKCHNP
                     QPFSLLDSQLYRFVILQLGAEETWFNFKMHHIISDGVSMNQAINEITGHYMDIVHGTGQ
                     NTPTKGNPYLDFIQVEQSYEQSDRYQKDKTYWTEKFADLPETTGLKSYNPLTLSTAARR
                     ESFILEHELYRGVTAFCEEHKISIFTFFLAALYIYLHKVTGEQDLSVGTLYANRTSKKE
                     KDTIGMFVSTVATRLLVEPESELLTFLQGVGKEQASILRHQRYPYNKIIQDLREQQSSL
                     DIQRLFGASIQYRTLSFSRFDEAIQQVDNDFCGDTVNDFDIHMVEMLDDDRLVLYLDYR
                     TELFSEQEIARIIRQFLSVAEHMVQGPHKRIQELSLISEEERAQIVDVFNATAVEYPHE
                     AGIHELFEEQAERTPNQTAVVFGNARLTYGELNVKANRLAHTLRGHGVTAEQTVGIVAE
                     RSTDMIVGMLAILKAGGAYVPIDPDYPAERVRYLLEDSGAKLLLVQRAEQQPEEYTGIV
                     LDLSDTKVYGAENDQSLNTVHWEQNEAYHADYTDYTNLKNEILKESSSSVVDSTAGEQE
                     RKFGNRLAYLMYTSGTTGQPKGVMVEHRNVVRLVKSTNYAQLDADTRILQTGAVVFDAS
                     TFEIWGALLNGGQLVLVSQDVILDAPKLKEAVRNHGITTMWLTAPLFNQLSQQDLELFE
                     GVQELLVGGDVLSVPHINRVLEAHPSLRIINGYGPTENTTFSTTHAIASVQLEAVPIGR
                     PIHNSTAYVVDRSMQLQPVGAWGELIVGGDGVARGYRNRPDLTAEKFVDSPFRSGERCY
                     RTGDLVRWNEDGTLEYKGRIDAQVKIRGYRIELGEVEAQLLKLEAVQEAVVIAREDEQG
                     QKQLCAYVVTLADIAASELRSALSQELPGYMVPSYFVQLEQLPLTPNGKVDRRALPQPE
                     GSVDSGEDYVAARNEVERTLVSVWQSVLGIEHIGVLDNFFHLGGDSIKAIQVSSRLLQN
                     GYKLEMRELFQYPTIAELNGRVQRVTRISDQGEVSGNVALTPIQRWFIEQQPSKPEHYN
                     QALMLHREDRLDETALHQALQRIVEHHDALRIVFRQNADGGYEAWNRGVQEGELYSLEV
                     VDFTNESDFAHLLENKASEIQSSIRLDEGPLVKLGLFRCPDGDHLLVAIHHLVVDGVSW
                     RILLEDLATGYEQSLRGESVRLPNKTDSFRLWADQLSTYANSSALEKERAYWQQLEQAA
                     ATQAALPKDYTCKSEVKPQLRDDRTLTVAWTIEETEQFLKQAHRAYNTEANDLLLTALG
                     TAIQEWAGIRQVLVNLEGHGREAILPDMDITRTIGWFTSQFPVVLDMGEDRNVGRRVKS
                     VKEGLRRLPHKGIGYGILRYLSAPREGEAAFVAEPEISFNYLGQFDQDLKHNAFGMSPY
                     SIGASISDTLTKRYTLDINGMITDGALELTISYSRKTFMK"
     source          1..10372
                     /collection_date="2019"
                     /db_xref="taxon:2962041"
                     /geo_loc_name="Portugal: Grandola, Mine of Lousal"
                     /isolation_source="Mine wall"
                     /mol_type="genomic DNA"
                     /organism="Paenibacillus sp. Lou8.1"
                     /strain="Lou8.1"
                     /submitter_seqid="NODE_33_length_10372_cov_148.123944"
     region          1..10372
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10372
                     /SMILES="NC(C(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10372
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:4503](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4503)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(4..318)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26270_TIGR01720.1"
                     /evalue="2.30E-35"
                     /label="NLX78_RS26270_TIGR01720.1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1500"
                     /protein_start="1395"
                     /score="113.7"
                     /tool="antismash"
                     /translation="VGRRVKSVKEGLRRLPHKGIGYGILRYLSAPREGEAAFVAEPEIS
                     FNYLGQFDQDLKHNAFGMSPYSIGASISDTLTKRYTLDINGMITDGALELTISYSRKTF
                     M"
     aSModule        complement(4..4479)
                     /complete
                     /domains="nrpspksdomains_NLX78_RS26270_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_NLX78_RS26270_AMP-binding.1"
                     /domains="nrpspksdomains_NLX78_RS26270_PCP.1"
                     /domains="nrpspksdomains_NLX78_RS26270_Epimerization.1"
                     /domains="nrpspksdomains_NLX78_RS26270_TIGR01720.1"
                     /locus_tags="NLX78_RS26270"
                     /monomer_pairings="Val -> D-Val"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(166..195)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0014"
                     /evalue="5.00E-03"
                     /label="NRPS-E7"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1446"
                     /protein_start="1436"
                     /score="9.5"
                     /tool="antismash"
                     /translation="PEISFNYLGQ"
     CDS_motif       complement(235..297)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0013"
                     /evalue="1.70E-10"
                     /label="NRPS-E6"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1423"
                     /protein_start="1402"
                     /score="33.0"
                     /tool="antismash"
                     /translation="VKEGLRRLPHKGIGYGILRYL"
     aSDomain        complement(331..1254)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26270_Epimerization.1"
                     /evalue="5.30E-151"
                     /label="NLX78_RS26270_Epimerization.1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1391"
                     /protein_start="1083"
                     /score="495.9"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GNVALTPIQRWFIEQQPSKPEHYNQALMLHREDRLDETALHQALQ
                     RIVEHHDALRIVFRQNADGGYEAWNRGVQEGELYSLEVVDFTNESDFAHLLENKASEIQ
                     SSIRLDEGPLVKLGLFRCPDGDHLLVAIHHLVVDGVSWRILLEDLATGYEQSLRGESVR
                     LPNKTDSFRLWADQLSTYANSSALEKERAYWQQLEQAAATQAALPKDYTCKSEVKPQLR
                     DDRTLTVAWTIEETEQFLKQAHRAYNTEANDLLLTALGTAIQEWAGIRQVLVNLEGHGR
                     EAILPDMDITRTIGWFTSQFPVVLDMG"
     CDS_motif       complement(340..390)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0012"
                     /evalue="1.30E-07"
                     /label="NRPS-E5"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1388"
                     /protein_start="1371"
                     /score="23.7"
                     /tool="antismash"
                     /translation="DITRTIGWFTSQFPVVL"
     CDS_motif       complement(808..873)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0011"
                     /evalue="9.90E-12"
                     /label="NRPS-E2"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1232"
                     /protein_start="1210"
                     /score="37.0"
                     /tool="antismash"
                     /translation="LLVAIHHLVVDGVSWRILLEDL"
     CDS_motif       complement(1078..1161)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0010"
                     /evalue="1.40E-07"
                     /label="C2_LCL_024-062"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1142"
                     /protein_start="1114"
                     /score="23.9"
                     /tool="antismash"
                     /translation="EDRLDETALHQALQRIVEHHDALRIVFR"
     CDS_motif       complement(1213..1242)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0009"
                     /evalue="1.70E-03"
                     /label="NRPS-E1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1097"
                     /protein_start="1087"
                     /score="11.5"
                     /tool="antismash"
                     /translation="LTPIQRWFIE"
     aSDomain        complement(1288..1488)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26270_PCP.1"
                     /evalue="2.20E-24"
                     /label="NLX78_RS26270_PCP.1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="1072"
                     /protein_start="1005"
                     /score="77.4"
                     /tool="antismash"
                     /translation="ERTLVSVWQSVLGIEHIGVLDNFFHLGGDSIKAIQVSSRLLQNGY
                     KLEMRELFQYPTIAELNGRVQR"
     CDS_motif       complement(1780..1845)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0008"
                     /evalue="6.80E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="908"
                     /protein_start="886"
                     /score="34.2"
                     /tool="antismash"
                     /translation="YKGRIDAQVKIRGYRIELGEVE"
     aSDomain        complement(1810..3111)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26270_AMP-binding.1"
                     /evalue="6.20E-113"
                     /label="NLX78_RS26270_AMP-binding.1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="898"
                     /protein_start="464"
                     /score="369.4"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAERTPNQTAVVFGNARLTYGELNVKANRLAHTLRGHGVTAE
                     QTVGIVAERSTDMIVGMLAILKAGGAYVPIDPDYPAERVRYLLEDSGAKLLLVQRAEQQ
                     PEEYTGIVLDLSDTKVYGAENDQSLNTVHWEQNEAYHADYTDYTNLKNEILKESSSSVV
                     DSTAGEQERKFGNRLAYLMYTSGTTGQPKGVMVEHRNVVRLVKSTNYAQLDADTRILQT
                     GAVVFDASTFEIWGALLNGGQLVLVSQDVILDAPKLKEAVRNHGITTMWLTAPLFNQLS
                     QQDLELFEGVQELLVGGDVLSVPHINRVLEAHPSLRIINGYGPTENTTFSTTHAIASVQ
                     LEAVPIGRPIHNSTAYVVDRSMQLQPVGAWGELIVGGDGVARGYRNRPDLTAEKFVDSP
                     FRSGERCYRTGDLVRWNEDGTLEYKGRIDAQVKIR"
     CDS_motif       complement(1927..2016)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0007"
                     /evalue="2.70E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="859"
                     /protein_start="829"
                     /score="51.6"
                     /tool="antismash"
                     /translation="PVGAWGELIVGGDGVARGYRNRPDLTAEKF"
     CDS_motif       complement(2518..2577)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0006"
                     /evalue="1.70E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="662"
                     /protein_start="642"
                     /score="32.7"
                     /tool="antismash"
                     /translation="AYLMYTSGTTGQPKGVMVEH"
     CDS_motif       complement(2884..2925)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0005"
                     /evalue="3.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="540"
                     /protein_start="526"
                     /score="20.0"
                     /tool="antismash"
                     /translation="LAILKAGGAYVPID"
     CDS_motif       complement(3448..3567)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0004"
                     /evalue="1.50E-08"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="352"
                     /protein_start="312"
                     /score="27.2"
                     /tool="antismash"
                     /translation="KEQASILRHQRYPYNKIIQDLREQQSSLDIQRLFGASIQY"
     aSDomain        complement(3613..4479)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26270_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.90E-75"
                     /label="NLX78_RS26270_Condensation_Starter.1"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="297"
                     /protein_start="8"
                     /score="246.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="FSLSQPQQRIWYTELLYPNRNTSTIIATVKIRGTVRIDALQQAMN
                     RVIAQNDSFRIKITAQDGIPYQYVEPFTAESIETLQVTPEEAKEWLKCHNPQPFSLLDS
                     QLYRFVILQLGAEETWFNFKMHHIISDGVSMNQAINEITGHYMDIVHGTGQNTPTKGNP
                     YLDFIQVEQSYEQSDRYQKDKTYWTEKFADLPETTGLKSYNPLTLSTAARRESFILEHE
                     LYRGVTAFCEEHKISIFTFFLAALYIYLHKVTGEQDLSVGTLYANRTSKKEKDTIGMFV
                     STVATRLL"
     CDS_motif       complement(3619..3708)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0003"
                     /evalue="1.90E-07"
                     /label="C5_LCL_267-296"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="295"
                     /protein_start="265"
                     /score="23.6"
                     /tool="antismash"
                     /translation="DLSVGTLYANRTSKKEKDTIGMFVSTVATR"
     CDS_motif       complement(4042..4107)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0002"
                     /evalue="1.20E-05"
                     /label="C3_LCL_132-143"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="154"
                     /protein_start="132"
                     /score="18.0"
                     /tool="antismash"
                     /translation="MHHIISDGVSMNQAINEITGHY"
     CDS_motif       complement(4306..4398)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26270_0001"
                     /evalue="1.50E-07"
                     /label="C2_starter_024-063"
                     /locus_tag="NLX78_RS26270"
                     /protein_end="66"
                     /protein_start="35"
                     /score="24.1"
                     /tool="antismash"
                     /translation="TVKIRGTVRIDALQQAMNRVIAQNDSFRIKI"
     gene            complement(4553..6418)
                     /locus_tag="NLX78_RS26275"
                     /old_locus_tag="NLX78_26260"
     CDS             complement(4553..6418)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430351.1"
                     /locus_tag="NLX78_RS26275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NLX78_26260"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_254351915.1"
                     /transl_table=11
                     /translation="MTFKQGFYEFKQLMSLMKPHRKWYFTGLVGNSLTNASITILLSFV
                     VHYLLNFAVNGDVQELYRAVYLVSGTFLALSLISPLCSYMYKRCVKLAIANVRTKLFGH
                     IVKLSYEKAEKHHSGDLVSRMTNDVQTLEQAYTVHIRSIILEISLFLGSLVILMTLDWR
                     FGIMAVLLGLASAWINAHFAKKMRKTSEELQGRMSRFTERLSDLLSGLQTVKLFGLRTK
                     VGGLCNVASEDINRISIRQGRHMGLQDMCNFVIEFITLGGVLVAGLILVSQKQMELGVL
                     GQIVQLQTGISTVFLELGAAVTLLQNSLAGLARIREILDESKEEEQFPAAKKKGPLTRL
                     IDPETAALSARESGEFYGDVETLRSDHAIEQTPAALELHDITFGYQPDHPTLQRLNLVV
                     PEGQVTALVGPSGGGKSTVMKLLLGFYPPDEGVLRVRGRLAEEFPLDEVRDLMAYVPQE
                     ATLFHGTVADNIRFGSPTATDEEVEAAAKTAYASGFIAELPEGYDTIVGERGANLSGGQ
                     RQRIAIARALLKNAPILLLDEATSALDAESEYEVQQGLNELMKDRTTLVIAHRLSTIER
                     ADTICVLADGELKEQGTHDQLLAEGGHYAELYELQFRKHPEEQTA"
     gene            complement(6393..8288)
                     /locus_tag="NLX78_RS26280"
                     /old_locus_tag="NLX78_26265"
     CDS             complement(6393..8288)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017428488.1"
                     /locus_tag="NLX78_RS26280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NLX78_26265"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_254351916.1"
                     /transl_table=11
                     /translation="MFRLNSAFRRLARYVRPYNLWVFITVIASLCLAAIDIVLGKAIEQ
                     MAQGTFSSTQNMIYLVVGMTLIGMPCKFVMRYASARFSVSALRDLRGDLVHKFCDLPVS
                     SVERKFTGDLVSRLTNNTAVLQNFFIQQFANLFYLPLVFTASLILLLLTSWKLILASLA
                     LMPIGMIITSLMSKPIEKYSADLQEKIAHLNAVGQDAIGGIPIVKAFNMQAVLLKKYMV
                     IMEEVVKRGLRLEKRYAAITPVGVMMLATPIILVIVYGGYLIQHGELNAGGIVLFLYLI
                     SFILQPIAMLPTIISQMKEAGGAAKHLFEVLDWQNERQDGEMTLVDQKRENEAPHLKDD
                     ANGEANRMSEGRAESDDDVDSVAVVSFEHLTFGYDEGNTVLKDISFQAGQGDMVAIVGA
                     SGGGKSTIFKLLCGFNEPGEHAGTIQLFGKSLTDWNLTSLRSHISMVSQEPSLFPATIA
                     ENIGYGRLAATREEIVEAAKAAHAHDFIVQLPEGYETLLSERGGGLSGGQKQRISIARA
                     LLKNAPLLLLDEPTSALDSQSEAQVQNAMNAVMQNRTVLVIAHRLSTVRQANRIIVLDQ
                     GSIVESGSHEELLGRDGVYRKLYLKNFEEEQHTAENGNSLPEERGERRDLQAGVL"
     misc_feature    complement(8378..8391)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: medium, score: 18.02"
                     /tool="antismash"
     gene            complement(8504..9328)
                     /locus_tag="NLX78_RS26285"
                     /old_locus_tag="NLX78_26270"
     CDS             complement(8504..9328)
                     /NRPS_PKS="Domain: Thioesterase (20-244). E-value: 1.6e-56.
                     Score: 184.2. Matches aSDomain:
                     nrpspksdomains_NLX78_RS26285_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016818477.1"
                     /locus_tag="NLX78_RS26285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NLX78_26270"
                     /product="thioesterase II family protein"
                     /protein_id="WP_254351917.1"
                     /sec_met_domain="Thioesterase (E-value: 1.2e-56, bitscore:
                     180.5, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 6.8e-11, bitscore:
                     31.3, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTRSQSWFAKTEVQPTAKLQLFCFPYAGGGAYIFNSWKSRFAPD
                     ITVLPIQLPGRESRSTEAPMDTLQDIVQSLVPAMAPYMHKPFAFFGHSMGALIAFETAR
                     QLYSKTGKLPVHIIISGKSAPHLPYSKKRLHDLADDSFTEELRLMQGTPEEVLQNAELM
                     QIIMPRLRADFKVCETYVYQPGNPLVCPMTVLGGMKDFEVSTESLHAWQQHTTSPMDVQ
                     MFEGNHFFIHEHEQEVMSAVENILSTSSPMAHAAAAHTAYNTSTLQPRFRQS"
     aSDomain        complement(8597..9268)
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NLX78_RS26285_Thioesterase.1"
                     /evalue="1.60E-56"
                     /label="NLX78_RS26285_Thioesterase.1"
                     /locus_tag="NLX78_RS26285"
                     /protein_end="244"
                     /protein_start="20"
                     /score="184.2"
                     /tool="antismash"
                     /translation="QLFCFPYAGGGAYIFNSWKSRFAPDITVLPIQLPGRESRSTEAPM
                     DTLQDIVQSLVPAMAPYMHKPFAFFGHSMGALIAFETARQLYSKTGKLPVHIIISGKSA
                     PHLPYSKKRLHDLADDSFTEELRLMQGTPEEVLQNAELMQIIMPRLRADFKVCETYVYQ
                     PGNPLVCPMTVLGGMKDFEVSTESLHAWQQHTTSPMDVQMFEGNHFFIHEHEQEVMSAV
                     EN"
     CDS_motif       complement(9011..9079)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_NLX78_RS26285_0001"
                     /evalue="7.40E-09"
                     /label="NRPS-te1"
                     /locus_tag="NLX78_RS26285"
                     /protein_end="106"
                     /protein_start="83"
                     /score="28.0"
                     /tool="antismash"
                     /translation="HKPFAFFGHSMGALIAFETARQL"
     misc_feature    complement(9679..9696)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.87"
                     /tool="antismash"
     misc_feature    complement(9690..9707)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: strong, score: 21.55"
                     /tool="antismash"
     misc_feature    complement(10037..10046)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     rRNA            complement(10287..>10372)
                     /db_xref="RFAM:RF00001"
                     /gene="rrf"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="NLX78_RS26290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="NLX78_26275"
                     /product="5S ribosomal RNA"
     gene            complement(10287..>10372)
                     /gene="rrf"
                     /locus_tag="NLX78_RS26290"
                     /old_locus_tag="NLX78_26275"
ORIGIN
        1 tttcatgaat gttttcctgc tgtagctgat cgtcagttcc agtgcaccgt ccgtgatcat
       61 gccgttgata tccagagtat accgcttggt caacgtatca ctaatagaag ctccgatgga
      121 ataaggagac atgccaaatg cattatgttt caaatcctga tcgaattgtc cgagatagtt
      181 aaagctgatt tccggctcgg cgacaaaagc ggcttcccct tccctaggcg cagaaagata
      241 tctcaaaatc ccgtatccaa ttcctttatg cggcaaacga cgcagccctt ccttgacgct
      301 cttgacacgc cgtcccacat tccgatcctc gcccatatcg agcacaaccg ggaattggct
      361 ggtaaaccaa ccgatcgtac gcgtaatatc catatccggc aaaatcgctt cccgaccgtg
      421 accctccaga tttacgagaa cctgacggat gcctgcccac tcctgaattg cggtgcccag
      481 agctgtcagc aataggtcgt tcgcttccgt attgtaggca cgatgagctt gtttcaagaa
      541 ttgttccgtt tcttctatcg tccacgctac ggtcagtgtg cggtcatctc gtagctgagg
      601 cttgacctcg gatttgcaag tgtaatcctt gggcaaagct gcttgtgtag ctgccgcttg
      661 ctccagctgt tgccagtacg cacgctcctt ctccaaagcc gaactgttcg catatgttga
      721 cagctggtct gcccacagac ggaaggaatc cgttttgttg ggtaaacgga cagactcgcc
      781 ccgtaaagat tgctcatagc cggttgccaa atcttccagc aaaatacgcc aagacacgcc
      841 gtcgaccacc aaatggtgga tcgctaccaa caaatggtct ccatccgggc aacggaacaa
      901 acccagtttc accaaagggc cttcatctag acggatactg ctttgaattt cgctggcttt
      961 gttctccagc aaatgagcaa agtcagactc atttgtaaaa tcaaccactt ccaggctgta
     1021 cagttctcct tcttgcacgc cacggttcca tgcttcatac ccaccgtccg cattttggcg
     1081 gaatacgatg cgcaaagcat catggtgttc gacaatgcgt tgcagtgcct gatgaagcgc
     1141 cgtttcgtcc aaacgatcct cacggtgcag catcagagcc tgattatagt gctcaggctt
     1201 tgatggctgc tgttcaatga accagcgttg aatcggtgtc aatgccacgt ttccactgac
     1261 ttctccttga tcggaaatgc gggtcacccg ctgcacccgt ccattcagct cagcaatcgt
     1321 cggatactgg aacagctctc tcatttcgag cttgtaacca ttttgcagca gtcttgaaga
     1381 cacctggatg gccttaatcg agtcgccccc cagatggaaa aagttatcca gcacaccaat
     1441 atgctcgata ccaagtacag attgccacac ggacactagc gtccgttcta cctcattacg
     1501 ggctgctacg taatcctcac cggaatccac gctgccctcc ggttgcggca acgcccgacg
     1561 atccactttg ccatttggtg tcaacggcaa ttgctccagc tgcacaaaat atgacggtac
     1621 catgtaaccc ggcaattctt ggctcaaggc gcttcgcagc tcactagccg cgatatccgc
     1681 aagagtgact acatatgcac agagctgctt ttgcccctgc tcatcctcac gtgcaatgac
     1741 gacggcttct tgcaccgctt ccagcttcaa cagctgcgct tccacctcac ccagctcgat
     1801 ccggtagccc cgaattttca cctgtgcgtc gatccgtcct ttgtattcca gcgtcccgtc
     1861 ctcattccag cgcaccaaat cgcctgtgcg gtagcagcgc tctccgctgc ggaacggact
     1921 atctacgaac ttttcagccg tcaaatccgg acggttccgg tagccacgtg ccaccccgtc
     1981 gccgccgacg atcaactccc cccatgcccc gacaggctga agctgcatgg aacggtctac
     2041 gacatacgct gtggagttgt ggatcggacg cccaatcggc accgcttcca attgcacgct
     2101 ggcaatcgca tgagttgtag aaaaagttgt attttctgtc ggcccatagc cgttaatgat
     2161 ccgcagggac ggatgagcct ccagcacccg gttaatgtgc ggtacggaca gcacatcacc
     2221 accgaccagc agttcttgta ccccctcgaa cagctccaag tcctgctgag atagctggtt
     2281 aaagagcggt gcagtcagcc acattgttgt gataccatgg ttgcgcacgg cttccttgag
     2341 ctttggagcg tccaaaatga catcctgact taccagcacg agctgtccac catttagtag
     2401 cgcaccccaa atttcgaagg tggacgcgtc aaagacaacg gcgccagttt gcaaaatgcg
     2461 tgtatcagca tctaattgcg catagtttgt acttttcacc aaacgcacaa cattgcgatg
     2521 ttctaccata acccctttgg gctgtccggt tgtgcccgaa gtgtacatca aataagctag
     2581 tcggtttcca aatttacgtt cttgttctcc agcggtgctg tctacgacag acgaggatga
     2641 ttcctttaaa atctcatttt tcagatttgt ataatcggta taatccgcat gataagcctc
     2701 gttttgctcc caatgtacgg tattcaagct ttggtcattc tctgccccgt acaccttagt
     2761 atcgctcaga tccagtacga ttccggtgta ttcctccggt tgctgttctg cgcgctgcac
     2821 cagcagcaac ttcgcccccg aatcttccaa caggtaacgt acacgttcag ccggataatc
     2881 aggatcaatc ggtacatatg cgccgcccgc tttgagaatc gccaacatgc cgacgatcat
     2941 atccgtggaa cgctcagcaa cgatacctac agtttgttcg gcggtaacac cgtgtccgcg
     3001 caacgtgtga gccagtcgat ttgcttttac attcagttcg ccataagtta aacgggcatt
     3061 tccgaacaca actgctgtct gattcggtgt acgttcagcc tgttcctcaa acaattcatg
     3121 aatgcctgct tcgtgtggat attccaccgc tgtagcattg aatacgtcga cgatttgcgc
     3181 ccgttcttcc tcactgatca gcgacaattc ctgtatccgc ttgtgtgggc cctgcaccat
     3241 atgctccgcc acactcaaaa attgtcgaat aattcgagca atttcctgtt cactaaacag
     3301 ttcggtacga taatctaggt acagcacgag tctatcatca tccagcattt caaccatgtg
     3361 aatatcgaag tcgttcaccg tatctccgca gaaatcgtta tcaacctgct gaatcgcttc
     3421 atcaaaccgg gagaaactca aggtacggta ctgaatggaa gcaccaaaca gacgctgaat
     3481 gtccagactg ctttgctgtt cacgcaaatc ctgaataatt ttgttgtacg ggtaacgctg
     3541 atgccgcaaa atggatgcct gctccttacc tacaccctgc aggaaggtca gcagttcgga
     3601 ttctggttcg accaacaggc gcgtagccac agtgctgacg aacataccga tggtatcctt
     3661 ttccttctta gacgtacggt tggcatagag tgtccccacg cttaagtcct gctctcccgt
     3721 cactttatgc aaataaatat acagagccgc caggaagaac gtaaaaatac taattttatg
     3781 ttcctcgcaa aaagccgtaa caccacgata caactcatgt tccagtataa agctctcccg
     3841 gcgtgcagct gtgctcagtg tcagcgggtt atatgatttc aaacctgtag tctctggcaa
     3901 atcagcaaac ttttccgtcc agtacgtttt gtccttttga taacggtctg attgctcata
     3961 gctttgctct acctggataa aatccagata gggattgccc ttggtcggcg tattttgacc
     4021 tgtaccatgc acaatatcca tataatgtcc ggtgatttca ttaatggcct gattcatgga
     4081 cacgccgtct gagataatat gatgcatttt aaaattaaac caggtttctt ctgccccgag
     4141 ctgtaagata acaaagcggt atagctgcga atccaacaat gagaacggct gtggattatg
     4201 gcactttaac cattcctttg cttcttccgg ggtaacttgg agcgtttcta tactttccgc
     4261 cgtaaacggc tctacatatt gatacggaat cccatcctga gcagtaattt tgatacgaaa
     4321 agaatcattt tgggcaatta cccggttcat tgcttgctgc aaggcatcta tacgaacagt
     4381 tcccctgatt tttactgttg caataattgt tgatgtgttc cgattaggat acagaagctc
     4441 cgtataccaa atccgttgct gtggctggct tagcgaaaac aattgaacat cttgctgtgt
     4501 catccgtctc tctcctcgca aaatagatat tggtaaaagg aaattcgcat atttatgcgg
     4561 tttgttcctc gggatgcttt cgaaattgaa gctcatacag ctctgcataa tgaccgccct
     4621 cggcaaggag ctgatcatgg gtaccctgct ctttcagctc gccatcggca aggacacaga
     4681 tcgtatccgc ccgttcaatc gtagacaatc gatgggcaat aactaatgtc gtacgatcct
     4741 tcatcagctc atttaagccc tgttggactt catattccga ttctgcatcc aaagccgagg
     4801 ttgcttcatc caacagcaaa atcggtgcat ttttgagcaa agcacgagca attgcgatcc
     4861 gctgtctttg accgccagac aaattggctc cgcgttcacc gacaatcgtg tcgtacccct
     4921 ctggcagctc ggctatgaag ccgctggcat aggctgtttt ggccgcagcc tctacttcct
     4981 catccgtggc agtcggtgag ccaaaacgga tattatccgc tactgtaccg tggaaaagcg
     5041 tagcctcctg tggtacatag gccatcaaat ccctgacctc atccaacgga aattcttctg
     5101 ccaagcgacc acgaacccgc agtacacctt cgtccggtgg gtaaaatccc aacaacagct
     5161 tcatgactgt gcttttgccg ccgccactcg ggccgacaag cgcggttacc tgtccttctg
     5221 gcacaaccaa gtttaacctt tgcagcgttg gatggtcagg ctgatagcca aaggtgatgt
     5281 cgtgcagctc cagcgcagcc ggagtctgtt caatggcatg gtctgaacgc aaggtctcaa
     5341 catcgccata gaattcccca gattcacggg ctgataaagc tgccgtctcg ggatcgataa
     5401 gcctagtcag cggtcccttc tttttagccg cggggaactg ctcttcttcc ttcgactcat
     5461 ccagaatttc gcggatacgc gccaagcctg ccaaggagtt ttgtaacagt gttacagccg
     5521 cccctagctc caaaaacacc gttgaaatac cggtttgcag ctgcacaatc tgtcctagta
     5581 ctcccagttc catttgcttc tgcgaaacca gaattaatcc ggcgacaagc acaccgccca
     5641 gcgtaatgaa ctcaatgaca aaattgcaca tatcctgcaa gcccatatga cgtccctgcc
     5701 ggatgctgat gcggtttatg tcctcgcttg caacattaca aagtccgcct acctttgtcc
     5761 tcaacccaaa caacttcacc gtctgcaaac cggaaagcaa atcactcaat cgttccgtga
     5821 acctgctcat ccgtccctgt aactcttcac tcgtctttcg cattttcttg gcgaaatgag
     5881 cattgatcca ggctgaagcc agcccaagta aaaccgccat aatcccaaat cgccaatcca
     5941 gcgtcatcaa aatgaccaac gagcccaaga aaagcgaaat ttccagtata atcgagcgga
     6001 tatgaacggt gtaagcctgc tccagcgtct gtacatcgtt cgtcatgcgg gatacaagat
     6061 cacccgaatg gtgtttttcg gctttttcgt acgatagctt gacaatgtga ccaaatagtt
     6121 tcgttcgtac attggcaatc gccagcttta cacaccgttt atacatatag ctgcacaggg
     6181 gggaaataag acttaatgcc aaaaatgtac cactgaccaa atagaccgct cggtatagct
     6241 cctgtacatc gccattaacc gcgaaattca gcaaataatg caccacaaaa gaaagtaaaa
     6301 tcgtgatgct agcattggtc agactattgc caaccagccc ggtaaaatac cattttcggt
     6361 gcggcttcat cagggacatc aactgcttaa attcataaaa cccctgcttg aaggtcacga
     6421 cgctcacccc tttcctcagg caacgaatta ccgttttccg ccgtatgctg ttcttcttca
     6481 aaattcttca aatacagctt tcggtacaca ccatcacgac ctagtaattc ctcatgagaa
     6541 cccgattcca caatgctccc ctgatcgagt acaataatgc ggttcgcctg acgtacggtt
     6601 gacagtcgat gagcaatgac cagtaccgtc cggttttgca ttaccgcatt catcgcattc
     6661 tgcacctgag cctccgactg actatcgagt gctgaagtcg gctcgtccaa tagaagcagc
     6721 ggagcatttt tcagtaatgc tcttgctatg gaaatacgct gtttttgacc gccagacaat
     6781 ccgcctcccc gctcgctcaa cagggtttca tagccctcgg gaagttgaac aataaaatca
     6841 tgcgcatgtg ccgctttcgc cgcctcgaca atttcttccc gagtcgccgc taaacgtcca
     6901 tatccgatat tttctgcaat ggtggccgga aaaagcgacg gctcctgaga taccatcgaa
     6961 atatgactgc gcaggcttgt taaattccaa tctgtcaaac tctttccaaa tagttgaatg
     7021 gttccagcat gctctccagg ttcattaaat ccgcaaagca gcttgaaaat cgtgctttta
     7081 cctccaccgc tggcaccgac tatcgccacc atatcccctt gccctgcctg gaagctgata
     7141 tccttcagca ctgtgttccc ttcatcgtag ccaaaggtca aatgctcaaa actgactaca
     7201 gcaacactat caacgtcatc atcactttct gctctgcctt cactcattcg gttggcttcc
     7261 ccatttgcat cgtctttcaa atgaggcgcc tcattctctc gcttctgatc taccaaggtc
     7321 atttcgccat cctgacgttc attctgccaa tccagtacct caaacaaatg ctttgcggct
     7381 ccacccgctt ccttcatttg cgaaatgatc gtcggcagca tggcaatcgg ctgcaatatg
     7441 aacgaaatca ggtataaaaa aagtacaatg cctccagcat tcagctctcc atgttgtatc
     7501 aggtatcctc cgtatacaat gaccaaaata atcggtgttg ctagcatcat cacacctacc
     7561 ggagtaatgg ctgcatagcg cttttctagc cgtaagccgc gcttgacgac ctcctccata
     7621 atcaccatat acttcttcag cagcacagct tgcatattga atgccttgac gatagggatt
     7681 cctccgattg catcttgccc cacagcgttc aaatgagcaa ttttctcctg aagatccgca
     7741 gaatattttt caatcggctt gctcattagt gatgttatga tcattcctat tggcatgagc
     7801 gcaagacttg ccaatatcag tttccagctt gtcagtaaaa gcaatatgag tgatgccgta
     7861 aacacaagcg gtaaataaaa cagattggca aactgctgga taaaaaagtt ctgtaatact
     7921 gccgtattat tggttagacg ggataccaaa tctccggtaa atttcctttc cacgctggaa
     7981 actggtaaat cgcaaaactt atgtacgaga tcgcctctca agtcgcgcag agcactaaca
     8041 ctaaatcgcg ctgaagcgta cctcatcaca aacttacagg gcattccaat tagcgtcata
     8101 ccaacaacca gatagatcat gttctgggta gaggagaatg tcccctgtgc catttgctca
     8161 atggccttac ccaaaacaat gtcaatagct gccaaacaca aggaggcgat caccgttatg
     8221 aaaacccata gattatacgg ccttacataa cgtgcgagtc tccgaaaagc agaatttaat
     8281 cgaaacaagg gcatccctct tttcgttgcc cacactttat cagtgcgaac tttttatatt
     8341 ggctgtggac gtcgttgaga taaactctgg gtttggatgc atgagtatcc gtgcagaata
     8401 aaatgcgatt tggaactatg ctggaatttg catcttggtg tgggttggtt gttgattgtt
     8461 gctgagatgg taataacgaa gtgtatcgtg atgtgtggga cattcaactc tgtctgaatc
     8521 gtggttgtag ggtgcttgtg ttgtaagctg tatgagctgc cgccgcgtgg gccatcggcg
     8581 aggatgtaga aagaatgttc tcgactgcgc tcataacctc ttgttcgtgt tcatggatga
     8641 aaaagtgatt accttcgaac atctgaacat ccataggtga tgtcgtatgt tgttgccagg
     8701 catggagtga ctctgtgcta acctcaaaat ccttcattcc gcctaaaacg gtcatcgggc
     8761 ataccagtgg atttcccggc tgataaacat aagtttcaca taccttaaaa tctgcacgca
     8821 gacgaggcat aataatttgc atcaattctg cattttgcag cacctcttca ggggttccct
     8881 gcattaaacg aagctcttcc gtgaaagaat cgtctgctaa atcatggagc cgttttttgg
     8941 aatatggaag atgtggcgcg gatttaccag atatgatgat atgcacaggt agctttccag
     9001 ttttgctgta taactgccgt gctgtttcga atgcaatgag cgcccccata ctgtgcccaa
     9061 aaaaagcgaa cggcttatgc atgtatggag ccatagcggg aaccaacgat tgaacgatgt
     9121 cttgaagagt atccattgga gcttctgttg aacggctttc acgtcctgga agttgtatag
     9181 gcagcacggt aatgtcaggc gcaaatctgg acttccaaga gttaaatatg taagcaccac
     9241 caccggcgta ggggaagcaa aatagttgga gcttcgcagt cggttggacc tctgttttcg
     9301 cgaaccaaga ttgcgatcga gtcgtcatat gaattcctcc atcctctatg catttcataa
     9361 ttttacagta aatccttata tctctttttt tcttccaaca ctttctaact gtttttaaat
     9421 atacaaactt taaaaccact tggcatggga caaaagggct aaattgatag agctcataaa
     9481 aataaaataa aatccatatt atgttatttg tttccaaaat tcaaaagcaa caatttcact
     9541 ttttgtatta gttatctgtc aatcatgatc tgttaattta gtttaaaacg aaattttagg
     9601 ttttcacgaa ttttgtaaaa ctaaactcat tatgatttac attgaagctc caccccctta
     9661 tcctacaaca attttatctt tacatgccat taacataaat gtaacaaatt caacctgata
     9721 tcatccgaca aattccgcct ttttccgcaa ctctctttaa cctcctaaat aatggattca
     9781 atttgtatca agctcacata agccaaaact aataactctt taattttgta ataaatattg
     9841 ttatttcata gtcctaaagg accctatttt gcatattttt ttctgactct tcgtctaaaa
     9901 aattcagttc ggattgagtt tttctctccc attgctatga attttctttt caaactctaa
     9961 tagaaacgtt tctatctcaa ttcaaaatta cctgttctct agaaatttat agccttactt
    10021 ttaatctttc accccctcaa aaaacagtaa cggcaaaaca gcaaggctcc ctgttgttgg
    10081 agcttgctgc attataaatt ttattatgtt tgacttcact atcggtcgtt atgcagagcg
    10141 ttgtaagctt tattgtagtt atcccccaat cttattaatt tagtgggatg gagtatatgt
    10201 atgggtttct tgtataattt ccaaaacgcg aaagccttat ggtatacaaa aagagcacag
    10261 cccattaagg ctgtgctctt ttatttgctt ggcggcgtcc tactctccca ggaccctgcg
    10321 gtccaagtac catcggcgct ggagggctta acggtcgtgt tcgggatggg ta
//
